PI4KA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PI4KA
Entrez Gene ID	282309
Full Name	phosphatidylinositol 4-kinase alpha
Synonyms	PIK4CA
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010814204.2	XP_010812506.1	phosphatidylinositol 4-kinase alpha isoform X1
XM_010814204.3	XP_010812506.2	phosphatidylinositol 4-kinase alpha isoform X1
NM_174577.2	NP_777002.1	phosphatidylinositol 4-kinase alpha

Pathways from BioSystems

Homologies

	PI4KA	XP_001086542.2
Gallus gallus (chicken)	PI4KA	XP_004945537.1
Bos taurus (cattle)	PI4KA	NP_777002.1
Drosophila melanogaster (fruit fly)	PI4KIIIalpha	NP_570014.3
Caenorhabditis elegans (roundworm)	CELE_Y75B8A.24	NP_499596.2
Oryza sativa (rice)	Os03g0711200	NP_001051054.1
Xenopus tropicalis (tropical clawed frog)	pi4ka	NP_001238804.1
Homo sapiens (human)	PI4KA	NP_477352.3
Pan troglodytes (chimpanzee)	PI4KA	XP_525639.3
Mus musculus (house mouse)	Pi4ka	NP_001001983.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010801	XP_318507.4
Canis lupus familiaris (dog)	PI4KA	XP_543569.5
Rattus norvegicus (Norway rat)	Pi4ka	NP_071637.1
Danio rerio (zebrafish)	pi4kaa	NP_001030144.1
Arabidopsis thaliana (thale cress)	ATPI4K ALPHA	NP_175359.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Isolation and molecular cloning of wortmannin-sensitive bovine type III phosphatidylinositol 4-kinases.
Balla T, Downing GJ, Jaffe H, Kim S, Z?lyomi A, Catt KJ
The Journal of biological chemistry272(29)18358-66(1997 Jul)

Identification of a 200 kDa polypeptide as type 3 phosphatidylinositol 4-kinase from bovine brain by partial protein and cDNA sequencing.
Gehrmann T, Vereb G, Schmidt M, Klix D, Meyer HE, Vars?nyi M, Heilmeyer LM Jr
Biochimica et biophysica acta1311(1)53-63(1996 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PI4KA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PI4KA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa224155	XM_010814204.3 Latest version!	Bos taurus phosphatidylinositol 4-kinase alpha (PI4KA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa41319	NM_174577.2 Latest version!	Bos taurus phosphatidylinositol 4-kinase alpha (PI4KA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa45423	XM_010814204.2 Latest version!	Bos taurus phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PI4KA ( XM_010814204.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812506.2 Bos taurus phosphatidylinositol 4-kinase alpha (PI4KA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa224155
Clone ID Related Accession (Same CDS sequence)	XM_010814204.3
Accession Version	XM_010814204.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphatidylinositol 4-kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814204.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGCGGCCCG GGGCGGCGGC GGCGGCGGCG GCGGCGGCAG CTTCGGCTCG 
GGCGCCGGCG GTGGCGCCTC CCGGGGTTTC TACTTCAACA CCGTCTTATC GCTGGCCCGC 
TCCCTGGCTG TGCAGAGACC GGCATCTCTG GAGAAGGTCC AAAAGCTGCT TTGTATGTGC 
CCAGTAGATT TCCATGGGAT CTTCCAGCTG GATGAGAGGC GGAGAGACGC AGTGATCGCA 
TTGGGCATCT TTCTCATTGA ATCTGACCTA CAGCACAAAG ACTCTGTGGT TCCTTACCTT 
CTTCGACTTC TTAAAGGTCT TCCGAAAGTG TATTGGGTAG AAGAGAGCAC AGCTCGGAAG 
GGCAGAGGTA CCCTCCCGGT GGCCGAGAGC TTCAGCTTCT GCTTGGTCAC TCTGCTGTCT 
GACGTGGCTT ACAGGGACCC TTCCCTGAGG GGTGAGATCC TAGAGGCGCT GCTGCAGGTC 
TTGCACGTGC TCCTGGGGAT GTGCCAGGCC CTGGAGATTC AAGAGAAAGA GTACCTCTGC 
AAGCACGCGG TCCCGTGCCT GCTGGGAATC GTGCGCGCCT TTGGGCGGCA CAGCAGCTCG 
GAGGACTCGA TCCTGTCCAG GCTGTTCCCC AGAGCTCCTG CGCACTCCCC TCGTGTCCCC 
GAGGAGCTGG AAGGTGTGCG CAGACGGTCC TTCAACGACT TCCGCTCCAT CCTCCCCAGC 
AGCCTGCTCA CTGTGTGCCA GGAGGGCAGC CTGAAGAGGA AGGCCAGCAG CGCATCCAGC 
GTCGCACAGG TCAGCCCTGA GCGTGGGGCA CCCCCTCCCA GCCCCCCTGG AGGATCTGCC 
TTTCACTGCC TCGAAGCCTC TTACTCACCC AATGGGAGCA CCTCGGAATC CGACTACTAC 
TTCTCTGCCG TCAGCTCCAG CTTCTCCGTC TCTCCTCTCT TCAACGGCGT CACCTACAAA 
GAGTTCAGCA TTCCCCTCGA GATGCTCCGG GAACTCTTAA ACCTGGTGAA GAAGATCGTG 
GAGGAGCCTG TTCTCAAGTC TCTGGACGCA GTGGTCACGG GTGTGATCGA GGCCAGCCCC 
AGTGCCGACC TCTGCTACGG CGCCTTCAGC GACCCCCTGT ACGTGGCTGT GCTCAGGATG 
CTGCGGGACA CCCTGTACTA CATGAGAGAC CTGCCCACCT CCTTTGTGAA GGAGGTGCAC 
GACTTCGTGC TGGAGCAGTT CAACAGCAGC CAGGGGGAGC TCCAGAAGAT CCTGCACGAT 
GCCGACCGGG CCCACAGCGA GCTGAGCCCC CTCAAGCTGC GCTGCCAGGC CAGTGCCGCC 
TGCGTGGACC TCATGGTCTG GGCAGTGAAG GACGAGCAGG GTGCAGAGAA TCTGTGCGTC 
AAGCTATCTG AGAAGCTGCA GTCCAAGACG TCCAGCAAGG TCATCATCGC GCACCTGCCA 
CTGCTCATCT GCTGTCTGCA GGGCCTGGGC CGCCTGTGCG AGCGGTTCCC GGTGGTGGTG 
CACTCGGTGA CACCCTCCCT GCGGGACTTC CTGGTGGTCC CATCCCCCGT GCTGGTGAAG 
CTGTACAAGT GCCACAGCCA GTACCACACA GTTGCCGGCA ATGACATAAA AATCAGCGTA 
ACCAATGAGC ATGCCGAGTC GACCCTAAAT GTGGCATCGG GGAAGAAGAG CCAGCCGTCC 
ATGTATGAGC AGCTTCGGGA CATTGCCATC GACAATGTCT GCAGGTGCCT GAAGGCGGGC 
CTGACCGTGG ACCCAGTGAT CGTGGAGGCC TTCCTGGCCA GCCTCTCCAA CCGGCTGTAC 
ATCTCCCAGG AGAGCGATAA GGATGCACAC CTGATTCCTG ACCACACCAT CCGAGCCTTG 
GGACACATCG CGGTGGCCCT GAGGGACACC CCAAAGGTCA TGGAGCCCAT TCTGCAGATC 
CTGCAGCAGA AATTCTGCCA GCCCCCCTCC CCGCTTGACG TGCTCATCAT CGACCAGCTG 
GGCTGCCTGG TCATCACCGG AAACCAATAT ATTTACCAGG AGGTGTGGAA CCTCTTTCAG 
CAGATCAGCG TGAAGGCCAG CTCGGTCGTG TACTCGGCCA CCAAGGACTA CAAGGACCAT 
GGCTACAGGC ACTGCTCGCT GGCGGTGATC AACGCCCTGG CCAACATCGC GGCCAACATC 
CAGGATGAGC ACCTCGTGGA CGAGCTGCTC ATGAACCTGC TGGAGCTGTT TGTGCAGCTG 
GGGCTGGAGG GCAAGCGGGC CAGTGAGCGG GCCAGCGAGA AGGGGCCCGC CCTCAAGGCC 
TCCAGCAGTG CGGGGAACCT GGGCGTGCTC ATCCCTGTGA TCGCAGTGCT CACCCGGCGG 
CTGCCGCCCA TCAAGGAGCC GAAGCCGAGG CTGCAGAAGC TCTTCCGGGA CTTCTGGCTG 
TACTCGGTGC TGATGGGGTT CGCCGTGGAG GGCTCAGGAC TCTGGCCCGA GGAGTGGTAC 
GAGGGGGTCT GTGAGATCGC CACCAAGTCC CCGCTGCTCA CCTTTCCCAG CAAAGAGCCT 
CTGCGGTCCG TCCTCCAGTA CAACTCGGCC ATGAAGAACG ACACGGTTAC GCCTGCCGAG 
CTGAGTGAGC TGCGCAGCAC CATCGTCAAC CTTCTGGACC CGGCCCCCGA GGTGTCCGCC 
CTCATCGGCA AGCTGGACTT CGCCATGTCC ACCTACCTGC TGTCCGTCTA CCGGCTGGAG 
TACATGAGGG TCCTGCGCTC CAGCGACCCC GCCCGCTTCC AGGTGATGTT CTGCTACTTC 
GAGGACAAGG CCATCCAGAA GGACAAGTCC GGGATGATGC AGTGTGTGAT CGCCGTCGCC 
GACAAGGTGT TCGACGCCTT CCTGAACATG ATGGCGGAGA AAGCCAAGAC CAAGGAGAAC 
GAGGAGGAGC TGGAGCGGCA CGCGCAGTTC CTGCTGGTCA GCTTCAACCA CGTCCACAAG 
CGCATCCGCA GGGTCGCCGA CAAGTACCTG TCTGGGCTGG TGGACAAGTT CCCCCACTTG 
CTCTGGAGCG GGACGGTGCT GAAGACCATG CTGGACATCC TGCAGACCCT GTCCCTGTCG 
CTGAGTGCTG ACATCCACAA GGACCAGCCA TACTACGACA TTCCCGACGT GCCCTACCGC 
ATCACCGTCC CGGACACCTA CGAGGCCCGA GAGAGCATCG TCAAAGACTT CGCCGCGCGC 
TGCGGGATGA TCCTTCAGGA GGCCATGAAG TGGGCGCCCA CGGTCACCAA GTCCCACCTG 
CAGGAGTACC TGAGCAAGCA CCAGAACTGG GTATCGGGGC TGTCGCAGCA CACGGGGCTG 
GCCATGGCCA CCGAGAGCGT CCTGCACTAC GCCGGCTACA ACAAGCAGAG CACGAGCCTC 
GGGGCGACAC AGCTCACAGA ACGGCCAGCC TGTGTCAAGA AGGACTACTC CAACTTCATG 
GCTTCCCTGA ACCTGCGCAA CCGCTATGCG GGCGAGGTGT ACGGGATGAT CCGGTTCTCG 
GACGCCACAG GCCACACATC GGACCTGAAC AAGATGATGG TCCAGGAGCT GAAGGCTGCG 
CTGGGCGCCG GCGACGCTCA GCAGTACACG CAGGCCATGT TCAAGCTGAC GGCCATGCTC 
ATCAGCAGCA GAGACTGTGA CCCGCAGCTC CTCCACCACC TGTGCTGGGG CCCCCTGCAG 
ATGTTCAACG AGCACGGCAT GGAGACCGCC CTGGCCTGCT GGGAGTGGCT GCTGGCCGGC 
AAGAACGGGG TGGAAGTGCC GTTCATGCGG GAGATGGCGG GGGCCTGGCA GATGACCGTG 
GAGCAGAAGT TCGGCCTGTT TTCTGCGGAG ATGAAGGAAG CAGACCCCTT GGCCGCATCA 
GAAGCCAGTC AGCCCAAACC GTGTGCCCCC GAGGTGACCC CTCACTACAT CTGGATCGAC 
TTCCTGGTAC AGCGGTTTGA GATTGCCAAG TACTGCAGTT CCGACCAGGT GGAGATCTTC 
TGCAGCCTCC TGCAGCGCTC CCTGTCCCTG AGCATCGGGG GCACCGCGGG CAGCATGAAC 
CGGCATGTGG CGGCCATCGG GCCCCGCTTC AAGCTGCTGA CCCTCGGCCT GTCCCTGCTG 
CACGCGGACG TGCTGCCCAA CGCCACCATC CGCAACGTGC TCCGCGAGAA GATCTACTCC 
ACCGCCTTTG ACTACTTCAG CTGCCCGCCG AGGTTCCCGA CCCAGGGGGA GAAGAGGCTG 
CGTGAGGACA TCAGCGTGAT GATCAAGTTC TGGACCGCAA TGTTCTCAGA CAAGAAGTAC 
CTGACCGCCA GCCAGCTCGT TCCCCCAGAC AACCAGGACA CCCGGAGCAA CCTGGACATC 
GCGGTCGGCT CTCGCCAGCA GGCCACGCAG GGCTGGATCA ACACGTACCC GCTGTCCAGC 
GGCATGTCCA CCATCTCCAA GAAATCGGGC ATGTCCAAGA AGACCAACCG GGGCTCCCAG 
CTGCACAAGT ATTACATGAA GCGCCGGACG CTGCTGCTGT CACTGCTGGC CACCGAGATC 
GAGCGCCTCA TCACCTGGTA CAACCCGCTG TCCGCCCCGG AGCTGGAGCT GGACCAGGCG 
GGGGAGAGCA GCGTGGCCAA CTGGAGGTCC AAGTACATCA GCCTGAGCGA GAAGCAGTGG 
AAGGACAACG TGAACCTCGC CTGGAGCATC TCGCCCCACC TGGCCGTGCA GCTGCCAGCC 
AGGTTCAAGA ACACGGAGGC TATCGGCAAC GAGGTGACAC GTCTGGTGCG CTTGGACCCA 
GGAGCCGTTA GTGACGTCCC GGAAGCCATC AAGTTCCTGG TCACCTGGCA CACCATCGAC 
GCCGACGCCC CCGAGCTCAG CCACGTGCTG TGCTGGGCGC CCGCGGACCC ACCCACAGGC 
CTGTCCTACT TCTCCAGCAT GTACCCGCCG CACCCCCTCA CGGCGCAGTA CGGGGTCAAG 
GTCCTGAGGT CCTTTCCGCC GGACGCCATC CTGTTCTACA TTCCCCAGAT TGTGCAGGCG 
CTCAGGTATG ACAAGATGGG CTACGTGCGG GAGTACATCC TGTGGGCCGC CTCCCAGTCC 
CAGCTACTGG CACACCAGTT CATCTGGAAC ATGAAGACCA ACATCTACGT GGACGAAGAA 
GGCCACCAGA AGGACCCCGA CATCGGTGAC CTCCTGGAGC AGCTGGTGGA GGAGATCACA 
GGCTCCCTCT CCGGCCCGGC CAAGGACTTC TACCAGCGGG AGTTCGACTT TTTCAACAAG 
ATCACCAACG TGTCGGCCAT CATCAAGCCC TACCCCAAAG GCGACGAGAG GAAGAAGGCC 
TGCCTGTCGG CCTTGTCTGA AGTGAAGGTG CAGCCTGGCT GCTACCTGCC CAGCAACCCC 
GAGGCCATCG TGCTGGACAT CGACTACAAG TCTGGGACGC CCATGCAGAG TGCCGCCAAG 
GCCCCGTATC TGGCGAAGTT CAAGGTGAAA CGTTGCGGGG TTAGTGAGCT TGAGAAGGAA 
GGCCTGCGGT GCCGCTCAGA CCCCGAGGAG GAGGGCAGCA TGCAGGAGGC CGACGGCCAG 
AAGATCTCCT GGCAGGCGGC CATCTTCAAA GTGGGGGACG ACTGCCGGCA GGACATGCTG 
GCCCTGCAGA TCATCGACCT CTTCAAAAAC ATCTTCCAGC TGGTCGGCCT GGACCTCTTC 
GTCTTCCCGT ACCGGGTGGT GGCCACCGCC CCCGGGTGCG GCGTCATCGA GTGCATCCCC 
GACTGCACCT CCCGGGACCA GCTGGGCCGC CAGACCGACT TTGGCATGTA CGACTACTTC 
ACGCGCCAGT ACGGGGACGA GTCCACACTG GCCTTCCAGC AGGCGCGCTA CAACTTCATC 
CGCAGCATGG CCGCCTACAG CCTCCTCCTG TTCCTGCTGC AGATCAAGGA CCGGCACAAC 
GGCAACATCA TGCTGGATAA GAAGGGCCAC CTCATCCACA TCGATTTCGG CTTCATGTTC 
GAAAGCTCCC CGGGCGGCAA CCTCGGCTGG GAGCCGGACA TCAAGCTGAC GGACGAGATG 
GTGATGATCA TGGGGGGCAA GATGGAGGCC ACGCCCTTCA AGTGGTTCAT GGAGATGTGT 
GTCCGTGGCT ACCTGGCTGT GCGGCCCTAC ATGGACGCTG TGGTCTCTCT GGTCACTCTC 
ATGCTGGACA CGGGCCTGCC CTGCTTCCGT GGCCAGACAA TCAAGCTCCT CAAGCACAGG 
TTCAGCCCCA ACATGACGGA GCGAGAGGCC GCGAACTTCA TCCTGAAGGT CATTCAGAAC 
TGCTTCCTCA GCAACAGGAG CCGGACCTAC GACATGATCC AGTACTATCA GAACGACATC 
CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812506.2
CDS	1..6309
Translation	MAAAAARGGGGGGGGGSFGSGAGGGASRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDSVVPYLLRLLKGLPKVYWVEESTARKGRGTLPVAESFSFCLVTLLSDVAYRDPSLRGEILEALLQVLHVLLGMCQALEIQEKEYLCKHAVPCLLGIVRAFGRHSSSEDSILSRLFPRAPAHSPRVPEELEGVRRRSFNDFRSILPSSLLTVCQEGSLKRKASSASSVAQVSPERGAPPPSPPGGSAFHCLEASYSPNGSTSESDYYFSAVSSSFSVSPLFNGVTYKEFSIPLEMLRELLNLVKKIVEEPVLKSLDAVVTGVIEASPSADLCYGAFSDPLYVAVLRMLRDTLYYMRDLPTSFVKEVHDFVLEQFNSSQGELQKILHDADRAHSELSPLKLRCQASAACVDLMVWAVKDEQGAENLCVKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVVPSPVLVKLYKCHSQYHTVAGNDIKISVTNEHAESTLNVASGKKSQPSMYEQLRDIAIDNVCRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEPKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIVNLLDPAPEVSALIGKLDFAMSTYLLSVYRLEYMRVLRSSDPARFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFLLVSFNHVHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDVPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLSKHQNWVSGLSQHTGLAMATESVLHYAGYNKQSTSLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVYGMIRFSDATGHTSDLNKMMVQELKAALGAGDAQQYTQAMFKLTAMLISSRDCDPQLLHHLCWGPLQMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWQMTVEQKFGLFSAEMKEADPLAASEASQPKPCAPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFCSLLQRSLSLSIGGTAGSMNRHVAAIGPRFKLLTLGLSLLHADVLPNATIRNVLREKIYSTAFDYFSCPPRFPTQGEKRLREDISVMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDIAVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGESSVANWRSKYISLSEKQWKDNVNLAWSISPHLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPADPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAASQSQLLAHQFIWNMKTNIYVDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDPEEEGSMQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHLIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	XM_010814204.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphatidylinositol 4-kinase alpha (PI4KA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814204.3

ORF Insert Sequence:

ATGGCGGCGG CCGCGGCCCG GGGCGGCGGC GGCGGCGGCG GCGGCGGCAG CTTCGGCTCG 
GGCGCCGGCG GTGGCGCCTC CCGGGGTTTC TACTTCAACA CCGTCTTATC GCTGGCCCGC 
TCCCTGGCTG TGCAGAGACC GGCATCTCTG GAGAAGGTCC AAAAGCTGCT TTGTATGTGC 
CCAGTAGATT TCCATGGGAT CTTCCAGCTG GATGAGAGGC GGAGAGACGC AGTGATCGCA 
TTGGGCATCT TTCTCATTGA ATCTGACCTA CAGCACAAAG ACTCTGTGGT TCCTTACCTT 
CTTCGACTTC TTAAAGGTCT TCCGAAAGTG TATTGGGTAG AAGAGAGCAC AGCTCGGAAG 
GGCAGAGGTA CCCTCCCGGT GGCCGAGAGC TTCAGCTTCT GCTTGGTCAC TCTGCTGTCT 
GACGTGGCTT ACAGGGACCC TTCCCTGAGG GGTGAGATCC TAGAGGCGCT GCTGCAGGTC 
TTGCACGTGC TCCTGGGGAT GTGCCAGGCC CTGGAGATTC AAGAGAAAGA GTACCTCTGC 
AAGCACGCGG TCCCGTGCCT GCTGGGAATC GTGCGCGCCT TTGGGCGGCA CAGCAGCTCG 
GAGGACTCGA TCCTGTCCAG GCTGTTCCCC AGAGCTCCTG CGCACTCCCC TCGTGTCCCC 
GAGGAGCTGG AAGGTGTGCG CAGACGGTCC TTCAACGACT TCCGCTCCAT CCTCCCCAGC 
AGCCTGCTCA CTGTGTGCCA GGAGGGCAGC CTGAAGAGGA AGGCCAGCAG CGCATCCAGC 
GTCGCACAGG TCAGCCCTGA GCGTGGGGCA CCCCCTCCCA GCCCCCCTGG AGGATCTGCC 
TTTCACTGCC TCGAAGCCTC TTACTCACCC AATGGGAGCA CCTCGGAATC CGACTACTAC 
TTCTCTGCCG TCAGCTCCAG CTTCTCCGTC TCTCCTCTCT TCAACGGCGT CACCTACAAA 
GAGTTCAGCA TTCCCCTCGA GATGCTCCGG GAACTCTTAA ACCTGGTGAA GAAGATCGTG 
GAGGAGCCTG TTCTCAAGTC TCTGGACGCA GTGGTCACGG GTGTGATCGA GGCCAGCCCC 
AGTGCCGACC TCTGCTACGG CGCCTTCAGC GACCCCCTGT ACGTGGCTGT GCTCAGGATG 
CTGCGGGACA CCCTGTACTA CATGAGAGAC CTGCCCACCT CCTTTGTGAA GGAGGTGCAC 
GACTTCGTGC TGGAGCAGTT CAACAGCAGC CAGGGGGAGC TCCAGAAGAT CCTGCACGAT 
GCCGACCGGG CCCACAGCGA GCTGAGCCCC CTCAAGCTGC GCTGCCAGGC CAGTGCCGCC 
TGCGTGGACC TCATGGTCTG GGCAGTGAAG GACGAGCAGG GTGCAGAGAA TCTGTGCGTC 
AAGCTATCTG AGAAGCTGCA GTCCAAGACG TCCAGCAAGG TCATCATCGC GCACCTGCCA 
CTGCTCATCT GCTGTCTGCA GGGCCTGGGC CGCCTGTGCG AGCGGTTCCC GGTGGTGGTG 
CACTCGGTGA CACCCTCCCT GCGGGACTTC CTGGTGGTCC CATCCCCCGT GCTGGTGAAG 
CTGTACAAGT GCCACAGCCA GTACCACACA GTTGCCGGCA ATGACATAAA AATCAGCGTA 
ACCAATGAGC ATGCCGAGTC GACCCTAAAT GTGGCATCGG GGAAGAAGAG CCAGCCGTCC 
ATGTATGAGC AGCTTCGGGA CATTGCCATC GACAATGTCT GCAGGTGCCT GAAGGCGGGC 
CTGACCGTGG ACCCAGTGAT CGTGGAGGCC TTCCTGGCCA GCCTCTCCAA CCGGCTGTAC 
ATCTCCCAGG AGAGCGATAA GGATGCACAC CTGATTCCTG ACCACACCAT CCGAGCCTTG 
GGACACATCG CGGTGGCCCT GAGGGACACC CCAAAGGTCA TGGAGCCCAT TCTGCAGATC 
CTGCAGCAGA AATTCTGCCA GCCCCCCTCC CCGCTTGACG TGCTCATCAT CGACCAGCTG 
GGCTGCCTGG TCATCACCGG AAACCAATAT ATTTACCAGG AGGTGTGGAA CCTCTTTCAG 
CAGATCAGCG TGAAGGCCAG CTCGGTCGTG TACTCGGCCA CCAAGGACTA CAAGGACCAT 
GGCTACAGGC ACTGCTCGCT GGCGGTGATC AACGCCCTGG CCAACATCGC GGCCAACATC 
CAGGATGAGC ACCTCGTGGA CGAGCTGCTC ATGAACCTGC TGGAGCTGTT TGTGCAGCTG 
GGGCTGGAGG GCAAGCGGGC CAGTGAGCGG GCCAGCGAGA AGGGGCCCGC CCTCAAGGCC 
TCCAGCAGTG CGGGGAACCT GGGCGTGCTC ATCCCTGTGA TCGCAGTGCT CACCCGGCGG 
CTGCCGCCCA TCAAGGAGCC GAAGCCGAGG CTGCAGAAGC TCTTCCGGGA CTTCTGGCTG 
TACTCGGTGC TGATGGGGTT CGCCGTGGAG GGCTCAGGAC TCTGGCCCGA GGAGTGGTAC 
GAGGGGGTCT GTGAGATCGC CACCAAGTCC CCGCTGCTCA CCTTTCCCAG CAAAGAGCCT 
CTGCGGTCCG TCCTCCAGTA CAACTCGGCC ATGAAGAACG ACACGGTTAC GCCTGCCGAG 
CTGAGTGAGC TGCGCAGCAC CATCGTCAAC CTTCTGGACC CGGCCCCCGA GGTGTCCGCC 
CTCATCGGCA AGCTGGACTT CGCCATGTCC ACCTACCTGC TGTCCGTCTA CCGGCTGGAG 
TACATGAGGG TCCTGCGCTC CAGCGACCCC GCCCGCTTCC AGGTGATGTT CTGCTACTTC 
GAGGACAAGG CCATCCAGAA GGACAAGTCC GGGATGATGC AGTGTGTGAT CGCCGTCGCC 
GACAAGGTGT TCGACGCCTT CCTGAACATG ATGGCGGAGA AAGCCAAGAC CAAGGAGAAC 
GAGGAGGAGC TGGAGCGGCA CGCGCAGTTC CTGCTGGTCA GCTTCAACCA CGTCCACAAG 
CGCATCCGCA GGGTCGCCGA CAAGTACCTG TCTGGGCTGG TGGACAAGTT CCCCCACTTG 
CTCTGGAGCG GGACGGTGCT GAAGACCATG CTGGACATCC TGCAGACCCT GTCCCTGTCG 
CTGAGTGCTG ACATCCACAA GGACCAGCCA TACTACGACA TTCCCGACGT GCCCTACCGC 
ATCACCGTCC CGGACACCTA CGAGGCCCGA GAGAGCATCG TCAAAGACTT CGCCGCGCGC 
TGCGGGATGA TCCTTCAGGA GGCCATGAAG TGGGCGCCCA CGGTCACCAA GTCCCACCTG 
CAGGAGTACC TGAGCAAGCA CCAGAACTGG GTATCGGGGC TGTCGCAGCA CACGGGGCTG 
GCCATGGCCA CCGAGAGCGT CCTGCACTAC GCCGGCTACA ACAAGCAGAG CACGAGCCTC 
GGGGCGACAC AGCTCACAGA ACGGCCAGCC TGTGTCAAGA AGGACTACTC CAACTTCATG 
GCTTCCCTGA ACCTGCGCAA CCGCTATGCG GGCGAGGTGT ACGGGATGAT CCGGTTCTCG 
GACGCCACAG GCCACACATC GGACCTGAAC AAGATGATGG TCCAGGAGCT GAAGGCTGCG 
CTGGGCGCCG GCGACGCTCA GCAGTACACG CAGGCCATGT TCAAGCTGAC GGCCATGCTC 
ATCAGCAGCA GAGACTGTGA CCCGCAGCTC CTCCACCACC TGTGCTGGGG CCCCCTGCAG 
ATGTTCAACG AGCACGGCAT GGAGACCGCC CTGGCCTGCT GGGAGTGGCT GCTGGCCGGC 
AAGAACGGGG TGGAAGTGCC GTTCATGCGG GAGATGGCGG GGGCCTGGCA GATGACCGTG 
GAGCAGAAGT TCGGCCTGTT TTCTGCGGAG ATGAAGGAAG CAGACCCCTT GGCCGCATCA 
GAAGCCAGTC AGCCCAAACC GTGTGCCCCC GAGGTGACCC CTCACTACAT CTGGATCGAC 
TTCCTGGTAC AGCGGTTTGA GATTGCCAAG TACTGCAGTT CCGACCAGGT GGAGATCTTC 
TGCAGCCTCC TGCAGCGCTC CCTGTCCCTG AGCATCGGGG GCACCGCGGG CAGCATGAAC 
CGGCATGTGG CGGCCATCGG GCCCCGCTTC AAGCTGCTGA CCCTCGGCCT GTCCCTGCTG 
CACGCGGACG TGCTGCCCAA CGCCACCATC CGCAACGTGC TCCGCGAGAA GATCTACTCC 
ACCGCCTTTG ACTACTTCAG CTGCCCGCCG AGGTTCCCGA CCCAGGGGGA GAAGAGGCTG 
CGTGAGGACA TCAGCGTGAT GATCAAGTTC TGGACCGCAA TGTTCTCAGA CAAGAAGTAC 
CTGACCGCCA GCCAGCTCGT TCCCCCAGAC AACCAGGACA CCCGGAGCAA CCTGGACATC 
GCGGTCGGCT CTCGCCAGCA GGCCACGCAG GGCTGGATCA ACACGTACCC GCTGTCCAGC 
GGCATGTCCA CCATCTCCAA GAAATCGGGC ATGTCCAAGA AGACCAACCG GGGCTCCCAG 
CTGCACAAGT ATTACATGAA GCGCCGGACG CTGCTGCTGT CACTGCTGGC CACCGAGATC 
GAGCGCCTCA TCACCTGGTA CAACCCGCTG TCCGCCCCGG AGCTGGAGCT GGACCAGGCG 
GGGGAGAGCA GCGTGGCCAA CTGGAGGTCC AAGTACATCA GCCTGAGCGA GAAGCAGTGG 
AAGGACAACG TGAACCTCGC CTGGAGCATC TCGCCCCACC TGGCCGTGCA GCTGCCAGCC 
AGGTTCAAGA ACACGGAGGC TATCGGCAAC GAGGTGACAC GTCTGGTGCG CTTGGACCCA 
GGAGCCGTTA GTGACGTCCC GGAAGCCATC AAGTTCCTGG TCACCTGGCA CACCATCGAC 
GCCGACGCCC CCGAGCTCAG CCACGTGCTG TGCTGGGCGC CCGCGGACCC ACCCACAGGC 
CTGTCCTACT TCTCCAGCAT GTACCCGCCG CACCCCCTCA CGGCGCAGTA CGGGGTCAAG 
GTCCTGAGGT CCTTTCCGCC GGACGCCATC CTGTTCTACA TTCCCCAGAT TGTGCAGGCG 
CTCAGGTATG ACAAGATGGG CTACGTGCGG GAGTACATCC TGTGGGCCGC CTCCCAGTCC 
CAGCTACTGG CACACCAGTT CATCTGGAAC ATGAAGACCA ACATCTACGT GGACGAAGAA 
GGCCACCAGA AGGACCCCGA CATCGGTGAC CTCCTGGAGC AGCTGGTGGA GGAGATCACA 
GGCTCCCTCT CCGGCCCGGC CAAGGACTTC TACCAGCGGG AGTTCGACTT TTTCAACAAG 
ATCACCAACG TGTCGGCCAT CATCAAGCCC TACCCCAAAG GCGACGAGAG GAAGAAGGCC 
TGCCTGTCGG CCTTGTCTGA AGTGAAGGTG CAGCCTGGCT GCTACCTGCC CAGCAACCCC 
GAGGCCATCG TGCTGGACAT CGACTACAAG TCTGGGACGC CCATGCAGAG TGCCGCCAAG 
GCCCCGTATC TGGCGAAGTT CAAGGTGAAA CGTTGCGGGG TTAGTGAGCT TGAGAAGGAA 
GGCCTGCGGT GCCGCTCAGA CCCCGAGGAG GAGGGCAGCA TGCAGGAGGC CGACGGCCAG 
AAGATCTCCT GGCAGGCGGC CATCTTCAAA GTGGGGGACG ACTGCCGGCA GGACATGCTG 
GCCCTGCAGA TCATCGACCT CTTCAAAAAC ATCTTCCAGC TGGTCGGCCT GGACCTCTTC 
GTCTTCCCGT ACCGGGTGGT GGCCACCGCC CCCGGGTGCG GCGTCATCGA GTGCATCCCC 
GACTGCACCT CCCGGGACCA GCTGGGCCGC CAGACCGACT TTGGCATGTA CGACTACTTC 
ACGCGCCAGT ACGGGGACGA GTCCACACTG GCCTTCCAGC AGGCGCGCTA CAACTTCATC 
CGCAGCATGG CCGCCTACAG CCTCCTCCTG TTCCTGCTGC AGATCAAGGA CCGGCACAAC 
GGCAACATCA TGCTGGATAA GAAGGGCCAC CTCATCCACA TCGATTTCGG CTTCATGTTC 
GAAAGCTCCC CGGGCGGCAA CCTCGGCTGG GAGCCGGACA TCAAGCTGAC GGACGAGATG 
GTGATGATCA TGGGGGGCAA GATGGAGGCC ACGCCCTTCA AGTGGTTCAT GGAGATGTGT 
GTCCGTGGCT ACCTGGCTGT GCGGCCCTAC ATGGACGCTG TGGTCTCTCT GGTCACTCTC 
ATGCTGGACA CGGGCCTGCC CTGCTTCCGT GGCCAGACAA TCAAGCTCCT CAAGCACAGG 
TTCAGCCCCA ACATGACGGA GCGAGAGGCC GCGAACTTCA TCCTGAAGGT CATTCAGAAC 
TGCTTCCTCA GCAACAGGAG CCGGACCTAC GACATGATCC AGTACTATCA GAACGACATC 
CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI4KA ( NM_174577.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777002.1 Bos taurus phosphatidylinositol 4-kinase alpha (PI4KA), mRNA.

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CloneID	OBa41319
Clone ID Related Accession (Same CDS sequence)	NM_174577.2
Accession Version	NM_174577.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Bos taurus(cattle)
Product	phosphatidylinositol 4-kinase alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U88532.1. On or before Mar 29, 2005 this sequence version replaced XM_581314.1, XM_615864.1, NM_174577.1. ##Evidence-Data-START## Transcript exon combination :: U88532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTGCCCAG TAGATTTCCA TGGGATCTTC CAGCTGGATG AGAGGCGGAG AGACGCAGTG 
ATCGCATTGG GCATCTTTCT CATTGAATCT GACCTACAGC ACAAAGACTC TGTGGTTCCT 
TACCTTCTTC GACTTCTTAA AGGTCTTCCG AAAGTGTATT GGGTAGAAGA GAGCACAGCT 
CGGAAGGGCA GAGGTACCCT CCCGGTGGCC GAGAGCTTGA GCTTCTGCTT GGTCACTCTG 
CTGTCTGACG TGGCTTACAG GGACCCTTCC CTGAGGGGTG AGATCCTAGA GGCGCTGCTG 
CAGGTCTTGC ACGTGCTCCT GGGGATGTGC CAGGCCCTGG AGATTCAAGA GAAAGAGTAC 
CTCTGCAAGC ACGCGGTCCC GTGCCTGCTG GGAATCGTGC GCGCCTTTGG GCGGCACAGC 
AGCTCGGAGG ACTCGCTCCT GTCCAGGCTG TTCCCCAGAG CTCCTGCGCA CTCCCCTCGT 
GTCCCCGAGG AGCTGGAAGG TGTGCGCAGA CGGTCCTTCA ACGACTTCCG CTCCATCCTC 
CCCAGCAGCC TGCTCACTGT GTGCCAGGAG GGCAGCCTGA AGAGGAAGGC CAGCAGCGCA 
TCCAGCGTCG CACAGGTCAG CCCTGAGCGT GGGGCACCCC CTCCCAGCCC CCCTGGAGGA 
TCTGCCTTTC ACTGCCTCGA AGCCTCTTAC TCACCCAATG GGAGCACCTC GGAATCCGAC 
TACTACTTCT CTGCCGTCAG CTCCAGCCTC TCCGTCTCTC CTCTCTTCAA CGGCGTCACC 
TACAAAGAGT TCAGCATTCC CCTCGAGATG CTCCGGGAAC TCTTAAACCT GGTGAAGAAG 
ATCGTGGAGG AGCCTGTGCT CAAGTCTCTG GACGCAGTGG TCACGGGTGT TATCGAGGCC 
AGCCCCAGTG CCGACCTCTG CCACGGCGCC TTCAGCGACC CCCTGTACGT GGCTGTGCTC 
AGGATGCTGC GGGACACCCT GTACTACATG AGAGACCTGC CCACCTCCTT TGTGAAGGAG 
GTGCACGACT TCGTGCTGGA GCAGTTCAAC AGCAGCCAGG GGGAGCTCCA GAAGATCCTG 
CACGATGCCG ACCGGGCCCA CAGCGAGCTG AGCCCCCTCA AGCTGCGCTG CCAGGCCAGT 
GCCGCCTGCG TGGACCTCAT GGTCTGGGCA GTGAAGGACG AGCAGGGTGC AGAGAATCTG 
TGCGTCAAGC TATCTGAGAA GCTGCAGTCC AAGACGTCCA GCAAGGTCAT CATCGCGCAG 
GTGCCACTGC TCATCTGCTG TCTGCAGGGC CTGGGCCGCC TGTGCGAGCG GTTCCCGGTG 
GTGGTGCACT CGGTGACACC CTCCCTGCGG GACTTCCTGG TGGTCCCATC CCCCGTGCTG 
GTGAAGCTGT ACAAGTGCCA CAGCCAGTAC CACACAGTTG CCGGCAATGA CATAAAAATC 
AGCGTAACCA ATGAGCATGC CGAGTCGACC CTAAATGTGG CATCGGGGAA GAAGAGCCAG 
CCGTCCATGT ATGAGCAGCT TCGGGACATT GCCATCGACA ATGTCTGCAG GTGCCTGAAG 
GCGGGCCTGA CCGAGGACCC AGTGATCGTG GAGGCCTTCC TGGCCAGCCT CTCCAACCGG 
CTGTACATCT CCCAGGAGAG CGATAAGGAT GCACACCTGA TTCCTGACCA CACCATCCGA 
GCCTTGGGAC ACATCGCGGT GGCCCTGAGG GACACCCCAA AGGTCATGGA GCCCATTCTG 
CAGATCCTGC AGCAGAAATT CTGCCAGCCC CCCTCCCCGC TTGACGTGCT CATCATCGAC 
CAGCTGGGCT GCCTGGTCAT CACCGGAAAC CAATATATTT ACCAGGAGGT GTGGAACCTC 
TTTCAGCAGA TCAGCGTGAA GGCCAGCTCG GTTGTGTACT CGGCCACCAA GGACTACAAG 
GACCATGGCT ACAGGCACTG CTCGCTGGCG GTGATCAACG CCCTGGCCAA CATCGCGGCC 
AACATCCAGG ATGAGCACCT CGTGGACGAG CTGCTCATGA ACCTGCTGGA GCTGTTTGTG 
CAGCTGGGGC TGGAGGGGAA GCGGGCCAGT GAGCGGGCCA GCGAGAAGGG GCCCGCCCTC 
AAGGCCTCCA GCAGTGCGGG GAACCTGGGC GTGCTCATCC CTGTGATCGC AGTGCTCACC 
CGGCGGCTGC CGCCCATCGA GGAGCCGAAG CCGAGGCTGC AGAAGCTCTT CCGGGACTTC 
TGGCTGTACT CGGTGCTGAT GGGGTTCGCC GTGGAGGGCT CAGGACTCTG GCCCGAGGAG 
TGGTACGAGG GGGTCTGTGA GATCGCCACC AAGTCCCCGC TGCTCACCTT TCCCAGCAAA 
GAGCCTCTGC GGTCCGTCCT CCAGTACAAC TCGGCCATGA AGAACGACAC GGTTACGCCT 
GCCGAGCTGA GTGAGCTGCG CAGCACCATC GTCAACCTTC TGGACCCGGC CCCCGAGGTG 
TCCGCCCTCA TCGGCAAGCT GGACTTCGCC ATGTCCACCT ACCTGCTGTC CGTCTACCGG 
CTGGAGTACA TGAGGGTCCT GCGCTCCAGC GACCCCGCCC GCTTCCAGGT GATGTTGTGC 
TACTTCGAGG ACAAGGCCAT CCAGAAGGAC AAGTCCGGGA TGATGCAGTG TGTGATCGCC 
GTCGCCGACA AGGTGTTCGA CGCCTTCCTG AACATGATGG CGGAGAAAGC CAAGACCAAG 
GAGAACGAGG AGGAGCTGGA GCGCACGCAG TTCCTGCTGG TCAGCTTCAA CCACGTCCAC 
AAGCGCATCC GCAGGGTCGC CGACAAGTAC CTGTCTGGGC TGGTGGACAA GTTCCCCCAC 
TTGCTCTGGA GCGGGACGGT GCTGAAGACC ATGCTGGACA TCCTGCAGAC CCTGTCCCTG 
TCGCTGAGTG CTGACATCCA CAAGGACCAG CCATACTACG ACATTCCCGA CGTGCCCTAC 
CGCATCACCG TCCCGGACAC CTACGAGGCC CGAGAGAGCA TCGTCAAAGA CTTCGCCGCG 
CGCTGCGGGA TGATCCTTCA GGAGGCCATG AAGTGGGCGC CCACGGTCAC CAAGTCCCAC 
CTGCAGGAGT ACCTGAGCAA GCACCAGAAC TGGGTATCGG GGCTGTCGCA GCACACGGGG 
CTGGCCATGG CCACCGAGAG CGTCCTGCAC TACTCCGGCT ACAACAAGCA GAGCACGAGC 
CTCGGGGCGA CACAGCTCAC AGAACGGCCA GCCTGTGTCA AGAAGGACTA CTCCAACTTC 
ATGGCTTCCC TGAACCTGCG CAACCGCTAT GCGGCCGAGG TGTACGGGAT GATCCGGTTC 
TCGGACGCCA CAGGCCACAC ATCGGACCTG AACAAGATGA TGGTCCAGGA GCTGAAGGCT 
GCGCTGGGCG CCGGCGACGC TCAGCAGTAC ACGCAGGCCA TGTTCAAGCT GACGGCCATG 
CTCATCAGCA GCAGAGACTG TGACCCGCAG CTCCTCCACC ACCTGTGCTG GGGCCCCCTG 
CAGATGTTCA ACGAGCACGG CATGGAGACC GCCCTGGCCT GCTGGGAGTG GCTGCTGGCC 
GGCAAGAACG GGGTGGAAGT GCCGTTCATG CGGGAGATGG CGGGGGCCTG GCAGATGACC 
GTGGAGCAGA AGTTCGGCCT GTTTTCTGCG GAGATGAAGG AAGCAGACCC CTTGGCCGCA 
TCAGAAGCCA GTCAGCCCAA ACCGTGTGCC CCCGAGGTGA CCCCTCACTA CATCTGGATC 
GACTTCCTGG TACAGCGGTT TGAGATTGCC AAGTACTGCA GTTCCGACCA GGTGGAGATC 
TTCTGCAGCC TCCTGCAGCG CTCCCTGTCC CTGAGCATCG GGGGCACCGC GGGCAGCATG 
AACCGGCATG TGGCGGCCAT CGGGCCCCGC TTCAAGCTGC TGACCCTCGG CCTGTCCCTG 
CTGCACGCGG ACGTGCTGCC CAACGCCACC ATCCGCAACG TGCTCCGCGA GAAGATCTAC 
TCCACCGCCT TTGACTACTT CAGCTGCCCG CCGAGGTTCC CGACCCAGGG GGAGAAGAGG 
CTGCGTGAGG ACATCAGCGT GATGATCAAG TTCTGGACCG CAATGTTCTC AGACAAGAAG 
TACCTGACCG CCAGCCAGCT CGTTCCCCCA GACAACCAGG ACACCCGGAG CAACCTGGAC 
ATCGCGGTCG GCTCTCGCCA GCAGGCCACG CAGGGCTGGA TCAACACGTA CCCGCTGTCC 
AGCGGCATGT CCACCATCTC CAAGAAATCG GGCATGTCCA AGAAGACCAA CCGGGGCTCC 
CAGCTGCACA AGTATTACAT GAAGCGCCGG ACGCTGCTGC TGTCACTGCT GGCCACCGAG 
ATCGAGCGCC TCATCACCTG GTACAACCCG CTGTCCGCCC CGGAGCTGGA GCTGGACCAG 
GCGGGGGAGA GCAGCGTGGC CAACTGGAGG TCCAAGTACA TCAGCCTGAG CGAGAAGCAG 
TGGAAGGACA ACGTGAACCT CGCCTGGAGC ATCTCGCCCC ACCTGGCCGT GCAGCTGCCA 
GCCAGGTTCA AGAACACGGA GGCTATCGGC AACGAGGTGA CACGTCTGGT GCGCTTGGAC 
CCAGGAGCCG TTAGTGACGT CCCGGAAGCC ATCAAGTTCC TGGTCACCTG GCACACCATC 
GACGCCGACG CCCCCGAGCT CAGCCACGTG CTGTGCTGGG CGCCCGCGGA CCCACCCACA 
GGCCTGTCCT ACTTCTCCAG CATGTCCCCG CCGCACCCCC TCACGGCGCA GTACGGGGTC 
AAGGTCCTGA GGTCCTTTCC GCCGGACGCC ATCCTGTTCT ACATTCCCCA GATTGTGCAG 
GCGCTCAGGT ATGACAAGAT GGGCTACGTG CGGGAGTACA TCCTGTGGGC CGCCTCCCAG 
TCCCAGCTAC TGGCACACCA GTTCATCTGG AACATGAAGA CCAACATCTA CGTGGACGAA 
GAAGGCCACC AGAAGGACCC CGACATCGGT GACCTCCTGG AGCAGCTGGT GGAGGAGATC 
ACAGGCTCCC TCTCCGGCCC GGCCAAGGAC TTCTACCAGC GGGAGTTCGA CTTTTTCAAC 
AAGATCACCA ACGTGTCGGC CATCATCAAG CCCTACCCCA AAGGCGACGA GAGGAAGAAG 
GCCTGCCTGT CGGCCTTGTC TGAAGTGAAG GTGCAGCCTG GCTGCTACCT GCCCAGCAAC 
CCCGAGGCCA TCGTGCTGGA CATCGACTAC AAGTCTGGGA CGCCCATGCA GAGTGCCGCC 
AAGGCCCCGT ATCTGGCGAA GTTCAAGGTG AAACGTTGCG GGGTTAGTGA GCTTGAGAAG 
GAAGGCCTGC GGTGCCGCTC AGACCCCGAG GAGGAGGGCA GCATGCAGGA GGCCGACGGC 
CAGAAGATCT CCTGGCAGGC GGCCATCTTC AAAGTGGGGG ACGACTGCCG GCAGGACATG 
CTGGCCCTGC AGATCATCGA CCTCTTCAAA AACATCTTCC AGCTGGTCGG CCTGGACCTC 
TTCGTCTTCC CGTACCGGGT GGTGGCCACC GCCCCCGGGT GCGGCGTCAT CGAGTGCATC 
CCCGACTGCA CCTCCCGGGA CCAGCTGGGC CGCCAGACCG ACTTTGGCAT GTACGACTAC 
TTCACGCGCC AGTACGGGGA CGAGTCCACA CTGGCCTTCC AGCAGGCGCG CTACAACTTC 
ATCCGCAGCA TGGCCGCCTA CAGCCTCCTC CTGTTCCTGC TGCAGATCAA GGACCGGCAC 
AACGGCAACA TCATGCTGGA TAAGAAGGGC CACCTCATCC ACATCGATTT CGGCTTCATG 
TTCGAAAGCT CCCCGGGCGG CAACCTCGGC TGGGAGCCGG ACATCAAGCT GACGGACGAG 
ATGGTGATGA TCATGGGGGG CAAGATGGAG GCCACGCCCT TCAAGTGGTT CATGGAGATG 
TGTGTCCGTG GCTACCTGGC TGTGCGGCCC TACATGGACG CTGTGGTCTC TCTGGTCACT 
CTCATGCTGG ACACGGGCCT GCCCTGCTTC CGTGGCCAGA CAATCAAGCT CCTCAAGCAC 
AGGTTCAGCC CCAACATGAC GGAGCGAGAG GCCGCGAACT TCATCCTGAA GGTCATTCAG 
AACTGCTTCC TCAGCAACAG GAGCCGGACC TACGACATGA TCCAGTACTA TCAGAACGAC 
ATCCCCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777002.1
CDS	60..6191
Translation	MCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDSVVPYLLRLLKGLPKVYWVEESTARKGRGTLPVAESLSFCLVTLLSDVAYRDPSLRGEILEALLQVLHVLLGMCQALEIQEKEYLCKHAVPCLLGIVRAFGRHSSSEDSLLSRLFPRAPAHSPRVPEELEGVRRRSFNDFRSILPSSLLTVCQEGSLKRKASSASSVAQVSPERGAPPPSPPGGSAFHCLEASYSPNGSTSESDYYFSAVSSSLSVSPLFNGVTYKEFSIPLEMLRELLNLVKKIVEEPVLKSLDAVVTGVIEASPSADLCHGAFSDPLYVAVLRMLRDTLYYMRDLPTSFVKEVHDFVLEQFNSSQGELQKILHDADRAHSELSPLKLRCQASAACVDLMVWAVKDEQGAENLCVKLSEKLQSKTSSKVIIAQVPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVVPSPVLVKLYKCHSQYHTVAGNDIKISVTNEHAESTLNVASGKKSQPSMYEQLRDIAIDNVCRCLKAGLTEDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIEEPKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIVNLLDPAPEVSALIGKLDFAMSTYLLSVYRLEYMRVLRSSDPARFQVMLCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERTQFLLVSFNHVHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDVPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLSKHQNWVSGLSQHTGLAMATESVLHYSGYNKQSTSLGATQLTERPACVKKDYSNFMASLNLRNRYAAEVYGMIRFSDATGHTSDLNKMMVQELKAALGAGDAQQYTQAMFKLTAMLISSRDCDPQLLHHLCWGPLQMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWQMTVEQKFGLFSAEMKEADPLAASEASQPKPCAPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFCSLLQRSLSLSIGGTAGSMNRHVAAIGPRFKLLTLGLSLLHADVLPNATIRNVLREKIYSTAFDYFSCPPRFPTQGEKRLREDISVMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDIAVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGESSVANWRSKYISLSEKQWKDNVNLAWSISPHLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPADPPTGLSYFSSMSPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAASQSQLLAHQFIWNMKTNIYVDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDPEEEGSMQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHLIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	NM_174577.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphatidylinositol 4-kinase alpha (PI4KA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174577.2

ORF Insert Sequence:

ATGTGCCCAG TAGATTTCCA TGGGATCTTC CAGCTGGATG AGAGGCGGAG AGACGCAGTG 
ATCGCATTGG GCATCTTTCT CATTGAATCT GACCTACAGC ACAAAGACTC TGTGGTTCCT 
TACCTTCTTC GACTTCTTAA AGGTCTTCCG AAAGTGTATT GGGTAGAAGA GAGCACAGCT 
CGGAAGGGCA GAGGTACCCT CCCGGTGGCC GAGAGCTTGA GCTTCTGCTT GGTCACTCTG 
CTGTCTGACG TGGCTTACAG GGACCCTTCC CTGAGGGGTG AGATCCTAGA GGCGCTGCTG 
CAGGTCTTGC ACGTGCTCCT GGGGATGTGC CAGGCCCTGG AGATTCAAGA GAAAGAGTAC 
CTCTGCAAGC ACGCGGTCCC GTGCCTGCTG GGAATCGTGC GCGCCTTTGG GCGGCACAGC 
AGCTCGGAGG ACTCGCTCCT GTCCAGGCTG TTCCCCAGAG CTCCTGCGCA CTCCCCTCGT 
GTCCCCGAGG AGCTGGAAGG TGTGCGCAGA CGGTCCTTCA ACGACTTCCG CTCCATCCTC 
CCCAGCAGCC TGCTCACTGT GTGCCAGGAG GGCAGCCTGA AGAGGAAGGC CAGCAGCGCA 
TCCAGCGTCG CACAGGTCAG CCCTGAGCGT GGGGCACCCC CTCCCAGCCC CCCTGGAGGA 
TCTGCCTTTC ACTGCCTCGA AGCCTCTTAC TCACCCAATG GGAGCACCTC GGAATCCGAC 
TACTACTTCT CTGCCGTCAG CTCCAGCCTC TCCGTCTCTC CTCTCTTCAA CGGCGTCACC 
TACAAAGAGT TCAGCATTCC CCTCGAGATG CTCCGGGAAC TCTTAAACCT GGTGAAGAAG 
ATCGTGGAGG AGCCTGTGCT CAAGTCTCTG GACGCAGTGG TCACGGGTGT TATCGAGGCC 
AGCCCCAGTG CCGACCTCTG CCACGGCGCC TTCAGCGACC CCCTGTACGT GGCTGTGCTC 
AGGATGCTGC GGGACACCCT GTACTACATG AGAGACCTGC CCACCTCCTT TGTGAAGGAG 
GTGCACGACT TCGTGCTGGA GCAGTTCAAC AGCAGCCAGG GGGAGCTCCA GAAGATCCTG 
CACGATGCCG ACCGGGCCCA CAGCGAGCTG AGCCCCCTCA AGCTGCGCTG CCAGGCCAGT 
GCCGCCTGCG TGGACCTCAT GGTCTGGGCA GTGAAGGACG AGCAGGGTGC AGAGAATCTG 
TGCGTCAAGC TATCTGAGAA GCTGCAGTCC AAGACGTCCA GCAAGGTCAT CATCGCGCAG 
GTGCCACTGC TCATCTGCTG TCTGCAGGGC CTGGGCCGCC TGTGCGAGCG GTTCCCGGTG 
GTGGTGCACT CGGTGACACC CTCCCTGCGG GACTTCCTGG TGGTCCCATC CCCCGTGCTG 
GTGAAGCTGT ACAAGTGCCA CAGCCAGTAC CACACAGTTG CCGGCAATGA CATAAAAATC 
AGCGTAACCA ATGAGCATGC CGAGTCGACC CTAAATGTGG CATCGGGGAA GAAGAGCCAG 
CCGTCCATGT ATGAGCAGCT TCGGGACATT GCCATCGACA ATGTCTGCAG GTGCCTGAAG 
GCGGGCCTGA CCGAGGACCC AGTGATCGTG GAGGCCTTCC TGGCCAGCCT CTCCAACCGG 
CTGTACATCT CCCAGGAGAG CGATAAGGAT GCACACCTGA TTCCTGACCA CACCATCCGA 
GCCTTGGGAC ACATCGCGGT GGCCCTGAGG GACACCCCAA AGGTCATGGA GCCCATTCTG 
CAGATCCTGC AGCAGAAATT CTGCCAGCCC CCCTCCCCGC TTGACGTGCT CATCATCGAC 
CAGCTGGGCT GCCTGGTCAT CACCGGAAAC CAATATATTT ACCAGGAGGT GTGGAACCTC 
TTTCAGCAGA TCAGCGTGAA GGCCAGCTCG GTTGTGTACT CGGCCACCAA GGACTACAAG 
GACCATGGCT ACAGGCACTG CTCGCTGGCG GTGATCAACG CCCTGGCCAA CATCGCGGCC 
AACATCCAGG ATGAGCACCT CGTGGACGAG CTGCTCATGA ACCTGCTGGA GCTGTTTGTG 
CAGCTGGGGC TGGAGGGGAA GCGGGCCAGT GAGCGGGCCA GCGAGAAGGG GCCCGCCCTC 
AAGGCCTCCA GCAGTGCGGG GAACCTGGGC GTGCTCATCC CTGTGATCGC AGTGCTCACC 
CGGCGGCTGC CGCCCATCGA GGAGCCGAAG CCGAGGCTGC AGAAGCTCTT CCGGGACTTC 
TGGCTGTACT CGGTGCTGAT GGGGTTCGCC GTGGAGGGCT CAGGACTCTG GCCCGAGGAG 
TGGTACGAGG GGGTCTGTGA GATCGCCACC AAGTCCCCGC TGCTCACCTT TCCCAGCAAA 
GAGCCTCTGC GGTCCGTCCT CCAGTACAAC TCGGCCATGA AGAACGACAC GGTTACGCCT 
GCCGAGCTGA GTGAGCTGCG CAGCACCATC GTCAACCTTC TGGACCCGGC CCCCGAGGTG 
TCCGCCCTCA TCGGCAAGCT GGACTTCGCC ATGTCCACCT ACCTGCTGTC CGTCTACCGG 
CTGGAGTACA TGAGGGTCCT GCGCTCCAGC GACCCCGCCC GCTTCCAGGT GATGTTGTGC 
TACTTCGAGG ACAAGGCCAT CCAGAAGGAC AAGTCCGGGA TGATGCAGTG TGTGATCGCC 
GTCGCCGACA AGGTGTTCGA CGCCTTCCTG AACATGATGG CGGAGAAAGC CAAGACCAAG 
GAGAACGAGG AGGAGCTGGA GCGCACGCAG TTCCTGCTGG TCAGCTTCAA CCACGTCCAC 
AAGCGCATCC GCAGGGTCGC CGACAAGTAC CTGTCTGGGC TGGTGGACAA GTTCCCCCAC 
TTGCTCTGGA GCGGGACGGT GCTGAAGACC ATGCTGGACA TCCTGCAGAC CCTGTCCCTG 
TCGCTGAGTG CTGACATCCA CAAGGACCAG CCATACTACG ACATTCCCGA CGTGCCCTAC 
CGCATCACCG TCCCGGACAC CTACGAGGCC CGAGAGAGCA TCGTCAAAGA CTTCGCCGCG 
CGCTGCGGGA TGATCCTTCA GGAGGCCATG AAGTGGGCGC CCACGGTCAC CAAGTCCCAC 
CTGCAGGAGT ACCTGAGCAA GCACCAGAAC TGGGTATCGG GGCTGTCGCA GCACACGGGG 
CTGGCCATGG CCACCGAGAG CGTCCTGCAC TACTCCGGCT ACAACAAGCA GAGCACGAGC 
CTCGGGGCGA CACAGCTCAC AGAACGGCCA GCCTGTGTCA AGAAGGACTA CTCCAACTTC 
ATGGCTTCCC TGAACCTGCG CAACCGCTAT GCGGCCGAGG TGTACGGGAT GATCCGGTTC 
TCGGACGCCA CAGGCCACAC ATCGGACCTG AACAAGATGA TGGTCCAGGA GCTGAAGGCT 
GCGCTGGGCG CCGGCGACGC TCAGCAGTAC ACGCAGGCCA TGTTCAAGCT GACGGCCATG 
CTCATCAGCA GCAGAGACTG TGACCCGCAG CTCCTCCACC ACCTGTGCTG GGGCCCCCTG 
CAGATGTTCA ACGAGCACGG CATGGAGACC GCCCTGGCCT GCTGGGAGTG GCTGCTGGCC 
GGCAAGAACG GGGTGGAAGT GCCGTTCATG CGGGAGATGG CGGGGGCCTG GCAGATGACC 
GTGGAGCAGA AGTTCGGCCT GTTTTCTGCG GAGATGAAGG AAGCAGACCC CTTGGCCGCA 
TCAGAAGCCA GTCAGCCCAA ACCGTGTGCC CCCGAGGTGA CCCCTCACTA CATCTGGATC 
GACTTCCTGG TACAGCGGTT TGAGATTGCC AAGTACTGCA GTTCCGACCA GGTGGAGATC 
TTCTGCAGCC TCCTGCAGCG CTCCCTGTCC CTGAGCATCG GGGGCACCGC GGGCAGCATG 
AACCGGCATG TGGCGGCCAT CGGGCCCCGC TTCAAGCTGC TGACCCTCGG CCTGTCCCTG 
CTGCACGCGG ACGTGCTGCC CAACGCCACC ATCCGCAACG TGCTCCGCGA GAAGATCTAC 
TCCACCGCCT TTGACTACTT CAGCTGCCCG CCGAGGTTCC CGACCCAGGG GGAGAAGAGG 
CTGCGTGAGG ACATCAGCGT GATGATCAAG TTCTGGACCG CAATGTTCTC AGACAAGAAG 
TACCTGACCG CCAGCCAGCT CGTTCCCCCA GACAACCAGG ACACCCGGAG CAACCTGGAC 
ATCGCGGTCG GCTCTCGCCA GCAGGCCACG CAGGGCTGGA TCAACACGTA CCCGCTGTCC 
AGCGGCATGT CCACCATCTC CAAGAAATCG GGCATGTCCA AGAAGACCAA CCGGGGCTCC 
CAGCTGCACA AGTATTACAT GAAGCGCCGG ACGCTGCTGC TGTCACTGCT GGCCACCGAG 
ATCGAGCGCC TCATCACCTG GTACAACCCG CTGTCCGCCC CGGAGCTGGA GCTGGACCAG 
GCGGGGGAGA GCAGCGTGGC CAACTGGAGG TCCAAGTACA TCAGCCTGAG CGAGAAGCAG 
TGGAAGGACA ACGTGAACCT CGCCTGGAGC ATCTCGCCCC ACCTGGCCGT GCAGCTGCCA 
GCCAGGTTCA AGAACACGGA GGCTATCGGC AACGAGGTGA CACGTCTGGT GCGCTTGGAC 
CCAGGAGCCG TTAGTGACGT CCCGGAAGCC ATCAAGTTCC TGGTCACCTG GCACACCATC 
GACGCCGACG CCCCCGAGCT CAGCCACGTG CTGTGCTGGG CGCCCGCGGA CCCACCCACA 
GGCCTGTCCT ACTTCTCCAG CATGTCCCCG CCGCACCCCC TCACGGCGCA GTACGGGGTC 
AAGGTCCTGA GGTCCTTTCC GCCGGACGCC ATCCTGTTCT ACATTCCCCA GATTGTGCAG 
GCGCTCAGGT ATGACAAGAT GGGCTACGTG CGGGAGTACA TCCTGTGGGC CGCCTCCCAG 
TCCCAGCTAC TGGCACACCA GTTCATCTGG AACATGAAGA CCAACATCTA CGTGGACGAA 
GAAGGCCACC AGAAGGACCC CGACATCGGT GACCTCCTGG AGCAGCTGGT GGAGGAGATC 
ACAGGCTCCC TCTCCGGCCC GGCCAAGGAC TTCTACCAGC GGGAGTTCGA CTTTTTCAAC 
AAGATCACCA ACGTGTCGGC CATCATCAAG CCCTACCCCA AAGGCGACGA GAGGAAGAAG 
GCCTGCCTGT CGGCCTTGTC TGAAGTGAAG GTGCAGCCTG GCTGCTACCT GCCCAGCAAC 
CCCGAGGCCA TCGTGCTGGA CATCGACTAC AAGTCTGGGA CGCCCATGCA GAGTGCCGCC 
AAGGCCCCGT ATCTGGCGAA GTTCAAGGTG AAACGTTGCG GGGTTAGTGA GCTTGAGAAG 
GAAGGCCTGC GGTGCCGCTC AGACCCCGAG GAGGAGGGCA GCATGCAGGA GGCCGACGGC 
CAGAAGATCT CCTGGCAGGC GGCCATCTTC AAAGTGGGGG ACGACTGCCG GCAGGACATG 
CTGGCCCTGC AGATCATCGA CCTCTTCAAA AACATCTTCC AGCTGGTCGG CCTGGACCTC 
TTCGTCTTCC CGTACCGGGT GGTGGCCACC GCCCCCGGGT GCGGCGTCAT CGAGTGCATC 
CCCGACTGCA CCTCCCGGGA CCAGCTGGGC CGCCAGACCG ACTTTGGCAT GTACGACTAC 
TTCACGCGCC AGTACGGGGA CGAGTCCACA CTGGCCTTCC AGCAGGCGCG CTACAACTTC 
ATCCGCAGCA TGGCCGCCTA CAGCCTCCTC CTGTTCCTGC TGCAGATCAA GGACCGGCAC 
AACGGCAACA TCATGCTGGA TAAGAAGGGC CACCTCATCC ACATCGATTT CGGCTTCATG 
TTCGAAAGCT CCCCGGGCGG CAACCTCGGC TGGGAGCCGG ACATCAAGCT GACGGACGAG 
ATGGTGATGA TCATGGGGGG CAAGATGGAG GCCACGCCCT TCAAGTGGTT CATGGAGATG 
TGTGTCCGTG GCTACCTGGC TGTGCGGCCC TACATGGACG CTGTGGTCTC TCTGGTCACT 
CTCATGCTGG ACACGGGCCT GCCCTGCTTC CGTGGCCAGA CAATCAAGCT CCTCAAGCAC 
AGGTTCAGCC CCAACATGAC GGAGCGAGAG GCCGCGAACT TCATCCTGAA GGTCATTCAG 
AACTGCTTCC TCAGCAACAG GAGCCGGACC TACGACATGA TCCAGTACTA TCAGAACGAC 
ATCCCCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI4KA ( XM_010814204.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812506.1 Bos taurus phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant X1, mRNA.

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CloneID	OBa45423
Clone ID Related Accession (Same CDS sequence)	XM_010814204.2
Accession Version	XM_010814204.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphatidylinositol 4-kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104461.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814204.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CGGCCGCGGC CCGGGGCGGC GGCGGCGGCG GCGGCGGCGG CAGCTTCGGC 
TCGGGCGCCG GCGGTGGCGC CTCCCGGGGT TTCTACTTCA ACACCGTCTT ATCGCTGGCC 
CGCTCCCTGG CTGTGCAGAG ACCGGCATCT CTGGAGAAGG TCCAAAAGCT GCTTTGTATG 
TGCCCAGTAG ATTTCCATGG GATCTTCCAG CTGGATGAGA GGCGGAGAGA CGCAGTGATC 
GCATTGGGCA TCTTTCTCAT TGAATCTGAC CTACAGCACA AAGACTCTGT GGTTCCTTAC 
CTTCTTCGAC TTCTTAAAGG TCTTCCGAAA GTGTATTGGG TAGAAGAGAG CACAGCTCGG 
AAGGGCAGAG GTACCCTCCC GGTGGCCGAG AGCTTCAGCT TCTGCTTGGT CACTCTGCTG 
TCTGACGTGG CTTACAGGGA CCCTTCCCTG AGGGGTGAGA TCCTAGAGGC GCTGCTGCAG 
GTCTTGCACG TGCTCCTGGG GATGTGCCAG GCCCTGGAGA TTCAAGAGAA AGAGTACCTC 
TGCAAGCACG CGGTCCCGTG CCTGCTGGGA ATCGTGCGCG CCTTTGGGCG GCACAGCAGC 
TCGGAGGACT CGATCCTGTC CAGGCTGTTC CCCAGAGCTC CTGCGCACTC CCCTCGTGTC 
CCCGAGGAGC TGGAAGGTGT GCGCAGACGG TCCTTCAACG ACTTCCGCTC CATCCTCCCC 
AGCAGCCTGC TCACTGTGTG CCAGGAGGGC AGCCTGAAGA GGAAGGCCAG CAGCGCATCC 
AGCGTCGCAC AGGTCAGCCC TGAGCGTGGG GCACCCCCTC CCAGCCCCCC TGGAGGATCT 
GCCTTTCACT GCCTCGAAGC CTCTTACTCA CCCAATGGGA GCACCTCGGA ATCCGACTAC 
TACTTCTCTG CCGTCAGCTC CAGCTTCTCC GTCTCTCCTC TCTTCAACGG CGTCACCTAC 
AAAGAGTTCA GCATTCCCCT CGAGATGCTC CGGGAACTCT TAAACCTGGT GAAGAAGATC 
GTGGAGGAGC CTGTTCTCAA GTCTCTGGAC GCAGTGGTCA CGGGTGTGAT CGAGGCCAGC 
CCCAGTGCCG ACCTCTGCTA CGGCGCCTTC AGCGACCCCC TGTACGTGGC TGTGCTCAGG 
ATGCTGCGGG ACACCCTGTA CTACATGAGA GACCTGCCCA CCTCCTTTGT GAAGGAGGTG 
CACGACTTCG TGCTGGAGCA GTTCAACAGC AGCCAGGGGG AGCTCCAGAA GATCCTGCAC 
GATGCCGACC GGGCCCACAG CGAGCTGAGC CCCCTCAAGC TGCGCTGCCA GGCCAGTGCC 
GCCTGCGTGG ACCTCATGGT CTGGGCAGTG AAGGACGAGC AGGGTGCAGA GAATCTGTGC 
GTCAAGCTAT CTGAGAAGCT GCAGTCCAAG ACGTCCAGCA AGGTCATCAT CGCGCACCTG 
CCACTGCTCA TCTGCTGTCT GCAGGGCCTG GGCCGCCTGT GCGAGCGGTT CCCGGTGGTG 
GTGCACTCGG TGACACCCTC CCTGCGGGAC TTCCTGGTGG TCCCATCCCC CGTGCTGGTG 
AAGCTGTACA AGTGCCACAG CCAGTACCAC ACAGTTGCCG GCAATGACAT AAAAATCAGC 
GTAACCAATG AGCATGCCGA GTCGACCCTA AATGTGGCAT CGGGGAAGAA GAGCCAGCCG 
TCCATGTATG AGCAGCTTCG GGACATTGCC ATCGACAATG TCTGCAGGTG CCTGAAGGCG 
GGCCTGACCG TGGACCCAGT GATCGTGGAG GCCTTCCTGG CCAGCCTCTC CAACCGGCTG 
TACATCTCCC AGGAGAGCGA TAAGGATGCA CACCTGATTC CTGACCACAC CATCCGAGCC 
TTGGGACACA TCGCGGTGGC CCTGAGGGAC ACCCCAAAGG TCATGGAGCC CATTCTGCAG 
ATCCTGCAGC AGAAATTCTG CCAGCCCCCC TCCCCGCTTG ACGTGCTCAT CATCGACCAG 
CTGGGCTGCC TGGTCATCAC CGGAAACCAA TATATTTACC AGGAGGTGTG GAACCTCTTT 
CAGCAGATCA GCGTGAAGGC CAGCTCGGTC GTGTACTCGG CCACCAAGGA CTACAAGGAC 
CATGGCTACA GGCACTGCTC GCTGGCGGTG ATCAACGCCC TGGCCAACAT CGCGGCCAAC 
ATCCAGGATG AGCACCTCGT GGACGAGCTG CTCATGAACC TGCTGGAGCT GTTTGTGCAG 
CTGGGGCTGG AGGGCAAGCG GGCCAGTGAG CGGGCCAGCG AGAAGGGGCC CGCCCTCAAG 
GCCTCCAGCA GTGCGGGGAA CCTGGGCGTG CTCATCCCTG TGATCGCAGT GCTCACCCGG 
CGGCTGCCGC CCATCAAGGA GCCGAAGCCG AGGCTGCAGA AGCTCTTCCG GGACTTCTGG 
CTGTACTCGG TGCTGATGGG GTTCGCCGTG GAGGGCTCAG GACTCTGGCC CGAGGAGTGG 
TACGAGGGGG TCTGTGAGAT CGCCACCAAG TCCCCGCTGC TCACCTTTCC CAGCAAAGAG 
CCTCTGCGGT CCGTCCTCCA GTACAACTCG GCCATGAAGA ACGACACGGT TACGCCTGCC 
GAGCTGAGTG AGCTGCGCAG CACCATCGTC AACCTTCTGG ACCCGGCCCC CGAGGTGTCC 
GCCCTCATCG GCAAGCTGGA CTTCGCCATG TCCACCTACC TGCTGTCCGT CTACCGGCTG 
GAGTACATGA GGGTCCTGCG CTCCAGCGAC CCCGCCCGCT TCCAGGTGAT GTTCTGCTAC 
TTCGAGGACA AGGCCATCCA GAAGGACAAG TCCGGGATGA TGCAGTGTGT GATCGCCGTC 
GCCGACAAGG TGTTCGACGC CTTCCTGAAC ATGATGGCGG AGAAAGCCAA GACCAAGGAG 
AACGAGGAGG AGCTGGAGCG GCACGCGCAG TTCCTGCTGG TCAGCTTCAA CCACGTCCAC 
AAGCGCATCC GCAGGGTCGC CGACAAGTAC CTGTCTGGGC TGGTGGACAA GTTCCCCCAC 
TTGCTCTGGA GCGGGACGGT GCTGAAGACC ATGCTGGACA TCCTGCAGAC CCTGTCCCTG 
TCGCTGAGTG CTGACATCCA CAAGGACCAG CCATACTACG ACATTCCCGA CGTGCCCTAC 
CGCATCACCG TCCCGGACAC CTACGAGGCC CGAGAGAGCA TCGTCAAAGA CTTCGCCGCG 
CGCTGCGGGA TGATCCTTCA GGAGGCCATG AAGTGGGCGC CCACGGTCAC CAAGTCCCAC 
CTGCAGGAGT ACCTGAGCAA GCACCAGAAC TGGGTATCGG GGCTGTCGCA GCACACGGGG 
CTGGCCATGG CCACCGAGAG CGTCCTGCAC TACGCCGGCT ACAACAAGCA GAGCACGAGC 
CTCGGGGCGA CACAGCTCAC AGAACGGCCA GCCTGTGTCA AGAAGGACTA CTCCAACTTC 
ATGGCTTCCC TGAACCTGCG CAACCGCTAT GCGGGCGAGG TGTACGGGAT GATCCGGTTC 
TCGGACGCCA CAGGCCACAC ATCGGACCTG AACAAGATGA TGGTCCAGGA GCTGAAGGCT 
GCGCTGGGCG CCGGCGACGC TCAGCAGTAC ACGCAGGCCA TGTTCAAGCT GACGGCCATG 
CTCATCAGCA GCAGAGACTG TGACCCGCAG CTCCTCCACC ACCTGTGCTG GGGCCCCCTG 
CAGATGTTCA ACGAGCACGG CATGGAGACC GCCCTGGCCT GCTGGGAGTG GCTGCTGGCC 
GGCAAGAACG GGGTGGAAGT GCCGTTCATG CGGGAGATGG CGGGGGCCTG GCAGATGACC 
GTGGAGCAGA AGTTCGGCCT GTTTTCTGCG GAGATGAAGG AAGCAGACCC CTTGGCCGCA 
TCAGAAGCCA GTCAGCCCAA ACCGTGTGCC CCCGAGGTGA CCCCTCACTA CATCTGGATC 
GACTTCCTGG TACAGCGGTT TGAGATTGCC AAGTACTGCA GTTCCGACCA GGTGGAGATC 
TTCTGCAGCC TCCTGCAGCG CTCCCTGTCC CTGAGCATCG GGGGCACCGC GGGCAGCATG 
AACCGGCATG TGGCGGCCAT CGGGCCCCGC TTCAAGCTGC TGACCCTCGG CCTGTCCCTG 
CTGCACGCGG ACGTGCTGCC CAACGCCACC ATCCGCAACG TGCTCCGCGA GAAGATCTAC 
TCCACCGCCT TTGACTACTT CAGCTGCCCG CCGAGGTTCC CGACCCAGGG GGAGAAGAGG 
CTGCGTGAGG ACATCAGCGT GATGATCAAG TTCTGGACCG CAATGTTCTC AGACAAGAAG 
TACCTGACCG CCAGCCAGCT CGTTCCCCCA GACAACCAGG ACACCCGGAG CAACCTGGAC 
ATCGCGGTCG GCTCTCGCCA GCAGGCCACG CAGGGCTGGA TCAACACGTA CCCGCTGTCC 
AGCGGCATGT CCACCATCTC CAAGAAATCG GGCATGTCCA AGAAGACCAA CCGGGGCTCC 
CAGCTGCACA AGTATTACAT GAAGCGCCGG ACGCTGCTGC TGTCACTGCT GGCCACCGAG 
ATCGAGCGCC TCATCACCTG GTACAACCCG CTGTCCGCCC CGGAGCTGGA GCTGGACCAG 
GCGGGGGAGA GCAGCGTGGC CAACTGGAGG TCCAAGTACA TCAGCCTGAG CGAGAAGCAG 
TGGAAGGACA ACGTGAACCT CGCCTGGAGC ATCTCGCCCC ACCTGGCCGT GCAGCTGCCA 
GCCAGGTTCA AGAACACGGA GGCTATCGGC AACGAGGTGA CACGTCTGGT GCGCTTGGAC 
CCAGGAGCCG TTAGTGACGT CCCGGAAGCC ATCAAGTTCC TGGTCACCTG GCACACCATC 
GACGCCGACG CCCCCGAGCT CAGCCACGTG CTGTGCTGGG CGCCCGCGGA CCCACCCACA 
GGCCTGTCCT ACTTCTCCAG CATGTACCCG CCGCACCCCC TCACGGCGCA GTACGGGGTC 
AAGGTCCTGA GGTCCTTTCC GCCGGACGCC ATCCTGTTCT ACATTCCCCA GATTGTGCAG 
GCGCTCAGGT ATGACAAGAT GGGCTACGTG CGGGAGTACA TCCTGTGGGC CGCCTCCCAG 
TCCCAGCTAC TGGCACACCA GTTCATCTGG AACATGAAGA CCAACATCTA CGTGGACGAA 
GAAGGCCACC AGAAGGACCC CGACATCGGT GACCTCCTGG AGCAGCTGGT GGAGGAGATC 
ACAGGCTCCC TCTCCGGCCC GGCCAAGGAC TTCTACCAGC GGGAGTTCGA CTTTTTCAAC 
AAGATCACCA ACGTGTCGGC CATCATCAAG CCCTACCCCA AAGGCGACGA GAGGAAGAAG 
GCCTGCCTGT CGGCCTTGTC TGAAGTGAAG GTGCAGCCTG GCTGCTACCT GCCCAGCAAC 
CCCGAGGCCA TCGTGCTGGA CATCGACTAC AAGTCTGGGA CGCCCATGCA GAGTGCCGCC 
AAGGCCCCGT ATCTGGCGAA GTTCAAGGTG AAACGTTGCG GGGTTAGTGA GCTTGAGAAG 
GAAGGCCTGC GGTGCCGCTC AGACCCCGAG GAGGAGGGCA GCATGCAGGA GGCCGACGGC 
CAGAAGATCT CCTGGCAGGC GGCCATCTTC AAAGTGGGGG ACGACTGCCG GCAGGACATG 
CTGGCCCTGC AGATCATCGA CCTCTTCAAA AACATCTTCC AGCTGGTCGG CCTGGACCTC 
TTCGTCTTCC CGTACCGGGT GGTGGCCACC GCCCCCGGGT GCGGCGTCAT CGAGTGCATC 
CCCGACTGCA CCTCCCGGGA CCAGCTGGGC CGCCAGACCG ACTTTGGCAT GTACGACTAC 
TTCACGCGCC AGTACGGGGA CGAGTCCACA CTGGCCTTCC AGCAGGCGCG CTACAACTTC 
ATCCGCAGCA TGGCCGCCTA CAGCCTCCTC CTGTTCCTGC TGCAGATCAA GGACCGGCAC 
AACGGCAACA TCATGCTGGA TAAGAAGGGC CACCTCATCC ACATCGATTT CGGCTTCATG 
TTCGAAAGCT CCCCGGGCGG CAACCTCGGC TGGGAGCCGG ACATCAAGCT GACGGACGAG 
ATGGTGATGA TCATGGGGGG CAAGATGGAG GCCACGCCCT TCAAGTGGTT CATGGAGATG 
TGTGTCCGTG GCTACCTGGC TGTGCGGCCC TACATGGACG CTGTGGTCTC TCTGGTCACT 
CTCATGCTGG ACACGGGCCT GCCCTGCTTC CGTGGCCAGA CAATCAAGCT CCTCAAGCAC 
AGGTTCAGCC CCAACATGAC GGAGCGAGAG GCCGCGAACT TCATCCTGAA GGTCATTCAG 
AACTGCTTCC TCAGCAACAG GAGCCGGACC TACGACATGA TCCAGTACTA TCAGAACGAC 
ATCCCCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812506.1
CDS	13..6324
Translation	MMAAAAARGGGGGGGGGSFGSGAGGGASRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDSVVPYLLRLLKGLPKVYWVEESTARKGRGTLPVAESFSFCLVTLLSDVAYRDPSLRGEILEALLQVLHVLLGMCQALEIQEKEYLCKHAVPCLLGIVRAFGRHSSSEDSILSRLFPRAPAHSPRVPEELEGVRRRSFNDFRSILPSSLLTVCQEGSLKRKASSASSVAQVSPERGAPPPSPPGGSAFHCLEASYSPNGSTSESDYYFSAVSSSFSVSPLFNGVTYKEFSIPLEMLRELLNLVKKIVEEPVLKSLDAVVTGVIEASPSADLCYGAFSDPLYVAVLRMLRDTLYYMRDLPTSFVKEVHDFVLEQFNSSQGELQKILHDADRAHSELSPLKLRCQASAACVDLMVWAVKDEQGAENLCVKLSEKLQSKTSSKVIIAHLPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVVPSPVLVKLYKCHSQYHTVAGNDIKISVTNEHAESTLNVASGKKSQPSMYEQLRDIAIDNVCRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEPKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIVNLLDPAPEVSALIGKLDFAMSTYLLSVYRLEYMRVLRSSDPARFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFLLVSFNHVHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDVPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLSKHQNWVSGLSQHTGLAMATESVLHYAGYNKQSTSLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVYGMIRFSDATGHTSDLNKMMVQELKAALGAGDAQQYTQAMFKLTAMLISSRDCDPQLLHHLCWGPLQMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWQMTVEQKFGLFSAEMKEADPLAASEASQPKPCAPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFCSLLQRSLSLSIGGTAGSMNRHVAAIGPRFKLLTLGLSLLHADVLPNATIRNVLREKIYSTAFDYFSCPPRFPTQGEKRLREDISVMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDIAVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGESSVANWRSKYISLSEKQWKDNVNLAWSISPHLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPADPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAASQSQLLAHQFIWNMKTNIYVDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDPEEEGSMQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHLIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	XM_010814204.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814204.2

ORF Insert Sequence:

ATGATGGCGG CGGCCGCGGC CCGGGGCGGC GGCGGCGGCG GCGGCGGCGG CAGCTTCGGC 
TCGGGCGCCG GCGGTGGCGC CTCCCGGGGT TTCTACTTCA ACACCGTCTT ATCGCTGGCC 
CGCTCCCTGG CTGTGCAGAG ACCGGCATCT CTGGAGAAGG TCCAAAAGCT GCTTTGTATG 
TGCCCAGTAG ATTTCCATGG GATCTTCCAG CTGGATGAGA GGCGGAGAGA CGCAGTGATC 
GCATTGGGCA TCTTTCTCAT TGAATCTGAC CTACAGCACA AAGACTCTGT GGTTCCTTAC 
CTTCTTCGAC TTCTTAAAGG TCTTCCGAAA GTGTATTGGG TAGAAGAGAG CACAGCTCGG 
AAGGGCAGAG GTACCCTCCC GGTGGCCGAG AGCTTCAGCT TCTGCTTGGT CACTCTGCTG 
TCTGACGTGG CTTACAGGGA CCCTTCCCTG AGGGGTGAGA TCCTAGAGGC GCTGCTGCAG 
GTCTTGCACG TGCTCCTGGG GATGTGCCAG GCCCTGGAGA TTCAAGAGAA AGAGTACCTC 
TGCAAGCACG CGGTCCCGTG CCTGCTGGGA ATCGTGCGCG CCTTTGGGCG GCACAGCAGC 
TCGGAGGACT CGATCCTGTC CAGGCTGTTC CCCAGAGCTC CTGCGCACTC CCCTCGTGTC 
CCCGAGGAGC TGGAAGGTGT GCGCAGACGG TCCTTCAACG ACTTCCGCTC CATCCTCCCC 
AGCAGCCTGC TCACTGTGTG CCAGGAGGGC AGCCTGAAGA GGAAGGCCAG CAGCGCATCC 
AGCGTCGCAC AGGTCAGCCC TGAGCGTGGG GCACCCCCTC CCAGCCCCCC TGGAGGATCT 
GCCTTTCACT GCCTCGAAGC CTCTTACTCA CCCAATGGGA GCACCTCGGA ATCCGACTAC 
TACTTCTCTG CCGTCAGCTC CAGCTTCTCC GTCTCTCCTC TCTTCAACGG CGTCACCTAC 
AAAGAGTTCA GCATTCCCCT CGAGATGCTC CGGGAACTCT TAAACCTGGT GAAGAAGATC 
GTGGAGGAGC CTGTTCTCAA GTCTCTGGAC GCAGTGGTCA CGGGTGTGAT CGAGGCCAGC 
CCCAGTGCCG ACCTCTGCTA CGGCGCCTTC AGCGACCCCC TGTACGTGGC TGTGCTCAGG 
ATGCTGCGGG ACACCCTGTA CTACATGAGA GACCTGCCCA CCTCCTTTGT GAAGGAGGTG 
CACGACTTCG TGCTGGAGCA GTTCAACAGC AGCCAGGGGG AGCTCCAGAA GATCCTGCAC 
GATGCCGACC GGGCCCACAG CGAGCTGAGC CCCCTCAAGC TGCGCTGCCA GGCCAGTGCC 
GCCTGCGTGG ACCTCATGGT CTGGGCAGTG AAGGACGAGC AGGGTGCAGA GAATCTGTGC 
GTCAAGCTAT CTGAGAAGCT GCAGTCCAAG ACGTCCAGCA AGGTCATCAT CGCGCACCTG 
CCACTGCTCA TCTGCTGTCT GCAGGGCCTG GGCCGCCTGT GCGAGCGGTT CCCGGTGGTG 
GTGCACTCGG TGACACCCTC CCTGCGGGAC TTCCTGGTGG TCCCATCCCC CGTGCTGGTG 
AAGCTGTACA AGTGCCACAG CCAGTACCAC ACAGTTGCCG GCAATGACAT AAAAATCAGC 
GTAACCAATG AGCATGCCGA GTCGACCCTA AATGTGGCAT CGGGGAAGAA GAGCCAGCCG 
TCCATGTATG AGCAGCTTCG GGACATTGCC ATCGACAATG TCTGCAGGTG CCTGAAGGCG 
GGCCTGACCG TGGACCCAGT GATCGTGGAG GCCTTCCTGG CCAGCCTCTC CAACCGGCTG 
TACATCTCCC AGGAGAGCGA TAAGGATGCA CACCTGATTC CTGACCACAC CATCCGAGCC 
TTGGGACACA TCGCGGTGGC CCTGAGGGAC ACCCCAAAGG TCATGGAGCC CATTCTGCAG 
ATCCTGCAGC AGAAATTCTG CCAGCCCCCC TCCCCGCTTG ACGTGCTCAT CATCGACCAG 
CTGGGCTGCC TGGTCATCAC CGGAAACCAA TATATTTACC AGGAGGTGTG GAACCTCTTT 
CAGCAGATCA GCGTGAAGGC CAGCTCGGTC GTGTACTCGG CCACCAAGGA CTACAAGGAC 
CATGGCTACA GGCACTGCTC GCTGGCGGTG ATCAACGCCC TGGCCAACAT CGCGGCCAAC 
ATCCAGGATG AGCACCTCGT GGACGAGCTG CTCATGAACC TGCTGGAGCT GTTTGTGCAG 
CTGGGGCTGG AGGGCAAGCG GGCCAGTGAG CGGGCCAGCG AGAAGGGGCC CGCCCTCAAG 
GCCTCCAGCA GTGCGGGGAA CCTGGGCGTG CTCATCCCTG TGATCGCAGT GCTCACCCGG 
CGGCTGCCGC CCATCAAGGA GCCGAAGCCG AGGCTGCAGA AGCTCTTCCG GGACTTCTGG 
CTGTACTCGG TGCTGATGGG GTTCGCCGTG GAGGGCTCAG GACTCTGGCC CGAGGAGTGG 
TACGAGGGGG TCTGTGAGAT CGCCACCAAG TCCCCGCTGC TCACCTTTCC CAGCAAAGAG 
CCTCTGCGGT CCGTCCTCCA GTACAACTCG GCCATGAAGA ACGACACGGT TACGCCTGCC 
GAGCTGAGTG AGCTGCGCAG CACCATCGTC AACCTTCTGG ACCCGGCCCC CGAGGTGTCC 
GCCCTCATCG GCAAGCTGGA CTTCGCCATG TCCACCTACC TGCTGTCCGT CTACCGGCTG 
GAGTACATGA GGGTCCTGCG CTCCAGCGAC CCCGCCCGCT TCCAGGTGAT GTTCTGCTAC 
TTCGAGGACA AGGCCATCCA GAAGGACAAG TCCGGGATGA TGCAGTGTGT GATCGCCGTC 
GCCGACAAGG TGTTCGACGC CTTCCTGAAC ATGATGGCGG AGAAAGCCAA GACCAAGGAG 
AACGAGGAGG AGCTGGAGCG GCACGCGCAG TTCCTGCTGG TCAGCTTCAA CCACGTCCAC 
AAGCGCATCC GCAGGGTCGC CGACAAGTAC CTGTCTGGGC TGGTGGACAA GTTCCCCCAC 
TTGCTCTGGA GCGGGACGGT GCTGAAGACC ATGCTGGACA TCCTGCAGAC CCTGTCCCTG 
TCGCTGAGTG CTGACATCCA CAAGGACCAG CCATACTACG ACATTCCCGA CGTGCCCTAC 
CGCATCACCG TCCCGGACAC CTACGAGGCC CGAGAGAGCA TCGTCAAAGA CTTCGCCGCG 
CGCTGCGGGA TGATCCTTCA GGAGGCCATG AAGTGGGCGC CCACGGTCAC CAAGTCCCAC 
CTGCAGGAGT ACCTGAGCAA GCACCAGAAC TGGGTATCGG GGCTGTCGCA GCACACGGGG 
CTGGCCATGG CCACCGAGAG CGTCCTGCAC TACGCCGGCT ACAACAAGCA GAGCACGAGC 
CTCGGGGCGA CACAGCTCAC AGAACGGCCA GCCTGTGTCA AGAAGGACTA CTCCAACTTC 
ATGGCTTCCC TGAACCTGCG CAACCGCTAT GCGGGCGAGG TGTACGGGAT GATCCGGTTC 
TCGGACGCCA CAGGCCACAC ATCGGACCTG AACAAGATGA TGGTCCAGGA GCTGAAGGCT 
GCGCTGGGCG CCGGCGACGC TCAGCAGTAC ACGCAGGCCA TGTTCAAGCT GACGGCCATG 
CTCATCAGCA GCAGAGACTG TGACCCGCAG CTCCTCCACC ACCTGTGCTG GGGCCCCCTG 
CAGATGTTCA ACGAGCACGG CATGGAGACC GCCCTGGCCT GCTGGGAGTG GCTGCTGGCC 
GGCAAGAACG GGGTGGAAGT GCCGTTCATG CGGGAGATGG CGGGGGCCTG GCAGATGACC 
GTGGAGCAGA AGTTCGGCCT GTTTTCTGCG GAGATGAAGG AAGCAGACCC CTTGGCCGCA 
TCAGAAGCCA GTCAGCCCAA ACCGTGTGCC CCCGAGGTGA CCCCTCACTA CATCTGGATC 
GACTTCCTGG TACAGCGGTT TGAGATTGCC AAGTACTGCA GTTCCGACCA GGTGGAGATC 
TTCTGCAGCC TCCTGCAGCG CTCCCTGTCC CTGAGCATCG GGGGCACCGC GGGCAGCATG 
AACCGGCATG TGGCGGCCAT CGGGCCCCGC TTCAAGCTGC TGACCCTCGG CCTGTCCCTG 
CTGCACGCGG ACGTGCTGCC CAACGCCACC ATCCGCAACG TGCTCCGCGA GAAGATCTAC 
TCCACCGCCT TTGACTACTT CAGCTGCCCG CCGAGGTTCC CGACCCAGGG GGAGAAGAGG 
CTGCGTGAGG ACATCAGCGT GATGATCAAG TTCTGGACCG CAATGTTCTC AGACAAGAAG 
TACCTGACCG CCAGCCAGCT CGTTCCCCCA GACAACCAGG ACACCCGGAG CAACCTGGAC 
ATCGCGGTCG GCTCTCGCCA GCAGGCCACG CAGGGCTGGA TCAACACGTA CCCGCTGTCC 
AGCGGCATGT CCACCATCTC CAAGAAATCG GGCATGTCCA AGAAGACCAA CCGGGGCTCC 
CAGCTGCACA AGTATTACAT GAAGCGCCGG ACGCTGCTGC TGTCACTGCT GGCCACCGAG 
ATCGAGCGCC TCATCACCTG GTACAACCCG CTGTCCGCCC CGGAGCTGGA GCTGGACCAG 
GCGGGGGAGA GCAGCGTGGC CAACTGGAGG TCCAAGTACA TCAGCCTGAG CGAGAAGCAG 
TGGAAGGACA ACGTGAACCT CGCCTGGAGC ATCTCGCCCC ACCTGGCCGT GCAGCTGCCA 
GCCAGGTTCA AGAACACGGA GGCTATCGGC AACGAGGTGA CACGTCTGGT GCGCTTGGAC 
CCAGGAGCCG TTAGTGACGT CCCGGAAGCC ATCAAGTTCC TGGTCACCTG GCACACCATC 
GACGCCGACG CCCCCGAGCT CAGCCACGTG CTGTGCTGGG CGCCCGCGGA CCCACCCACA 
GGCCTGTCCT ACTTCTCCAG CATGTACCCG CCGCACCCCC TCACGGCGCA GTACGGGGTC 
AAGGTCCTGA GGTCCTTTCC GCCGGACGCC ATCCTGTTCT ACATTCCCCA GATTGTGCAG 
GCGCTCAGGT ATGACAAGAT GGGCTACGTG CGGGAGTACA TCCTGTGGGC CGCCTCCCAG 
TCCCAGCTAC TGGCACACCA GTTCATCTGG AACATGAAGA CCAACATCTA CGTGGACGAA 
GAAGGCCACC AGAAGGACCC CGACATCGGT GACCTCCTGG AGCAGCTGGT GGAGGAGATC 
ACAGGCTCCC TCTCCGGCCC GGCCAAGGAC TTCTACCAGC GGGAGTTCGA CTTTTTCAAC 
AAGATCACCA ACGTGTCGGC CATCATCAAG CCCTACCCCA AAGGCGACGA GAGGAAGAAG 
GCCTGCCTGT CGGCCTTGTC TGAAGTGAAG GTGCAGCCTG GCTGCTACCT GCCCAGCAAC 
CCCGAGGCCA TCGTGCTGGA CATCGACTAC AAGTCTGGGA CGCCCATGCA GAGTGCCGCC 
AAGGCCCCGT ATCTGGCGAA GTTCAAGGTG AAACGTTGCG GGGTTAGTGA GCTTGAGAAG 
GAAGGCCTGC GGTGCCGCTC AGACCCCGAG GAGGAGGGCA GCATGCAGGA GGCCGACGGC 
CAGAAGATCT CCTGGCAGGC GGCCATCTTC AAAGTGGGGG ACGACTGCCG GCAGGACATG 
CTGGCCCTGC AGATCATCGA CCTCTTCAAA AACATCTTCC AGCTGGTCGG CCTGGACCTC 
TTCGTCTTCC CGTACCGGGT GGTGGCCACC GCCCCCGGGT GCGGCGTCAT CGAGTGCATC 
CCCGACTGCA CCTCCCGGGA CCAGCTGGGC CGCCAGACCG ACTTTGGCAT GTACGACTAC 
TTCACGCGCC AGTACGGGGA CGAGTCCACA CTGGCCTTCC AGCAGGCGCG CTACAACTTC 
ATCCGCAGCA TGGCCGCCTA CAGCCTCCTC CTGTTCCTGC TGCAGATCAA GGACCGGCAC 
AACGGCAACA TCATGCTGGA TAAGAAGGGC CACCTCATCC ACATCGATTT CGGCTTCATG 
TTCGAAAGCT CCCCGGGCGG CAACCTCGGC TGGGAGCCGG ACATCAAGCT GACGGACGAG 
ATGGTGATGA TCATGGGGGG CAAGATGGAG GCCACGCCCT TCAAGTGGTT CATGGAGATG 
TGTGTCCGTG GCTACCTGGC TGTGCGGCCC TACATGGACG CTGTGGTCTC TCTGGTCACT 
CTCATGCTGG ACACGGGCCT GCCCTGCTTC CGTGGCCAGA CAATCAAGCT CCTCAAGCAC 
AGGTTCAGCC CCAACATGAC GGAGCGAGAG GCCGCGAACT TCATCCTGAA GGTCATTCAG 
AACTGCTTCC TCAGCAACAG GAGCCGGACC TACGACATGA TCCAGTACTA TCAGAACGAC 
ATCCCCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Isolation and molecular cloning of wortmannin-sensitive bovine type III phosphatidylinositol 4-kinases.
The Journal of biological chemistry272(29)18358-66(1997 Jul)
Balla T,Downing GJ,Jaffe H,Kim S,Z?lyomi A,Catt KJ

Identification of a 200 kDa polypeptide as type 3 phosphatidylinositol 4-kinase from bovine brain by partial protein and cDNA sequencing.
Biochimica et biophysica acta1311(1)53-63(1996 Mar)
Gehrmann T,Vereb G,Schmidt M,Klix D,Meyer HE,Vars?nyi M,Heilmeyer LM Jr

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PI4KA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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