PI4KIIIalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PI4KIIIalpha
Entrez Gene ID	31247
Full Name	Phosphatidylinositol 4-kinase III alpha
Synonyms	CG10260,Dmel\CG10260,EG:BACR7C10.2,PI4K,PI4KIII[[alpha]],PI4KinaseIIIalpha,Pi4KIIIalpha,Stt4,ZW-2,l(1)17-59,l(1)3Ah,l(1)VA722,l(1)zw2,stt4,zw-2,zw2
General protein information	Preferred Names Phosphatidylinositol 4-kinase III alpha Names CG10260 gene product from transcript CG10260-RD CG10260-PC CG10260-PD PI4KIIIalpha-PC PI4KIIIalpha-PD lethal (1) 3Ah phosphatidylinositol 4-kinase III alpha phosphatidylinositol 4-kinase IIIalpha zeste-white 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3A9-3B1\|1-1.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272262.2	NP_001259191.1	phosphatidylinositol 4-kinase III alpha, isoform D
NM_130658.3	NP_570014.3	phosphatidylinositol 4-kinase III alpha, isoform C

Pathways from BioSystems

Homologies

	PI4KA	XP_001086542.2
Gallus gallus (chicken)	PI4KA	XP_004945537.1
Bos taurus (cattle)	PI4KA	NP_777002.1
Drosophila melanogaster (fruit fly)	PI4KIIIalpha	NP_570014.3
Caenorhabditis elegans (roundworm)	CELE_Y75B8A.24	NP_499596.2
Oryza sativa (rice)	Os03g0711200	NP_001051054.1
Xenopus tropicalis (tropical clawed frog)	pi4ka	NP_001238804.1
Homo sapiens (human)	PI4KA	NP_477352.3
Pan troglodytes (chimpanzee)	PI4KA	XP_525639.3
Mus musculus (house mouse)	Pi4ka	NP_001001983.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010801	XP_318507.4
Canis lupus familiaris (dog)	PI4KA	XP_543569.5
Rattus norvegicus (Norway rat)	Pi4ka	NP_071637.1
Danio rerio (zebrafish)	pi4kaa	NP_001030144.1
Arabidopsis thaliana (thale cress)	ATPI4K ALPHA	NP_175359.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study suggests the RBO-PI4KIIIalpha complex as a novel therapeutic target, and PI4KIIIalpha inhibitors as new drug candidates.
Title: Downregulation of RBO-PI4KIII? Facilitates A?

PI4KIIIalpha is a vital regulator of a functionally distinct pool of PtdIns4P that is essential for PtdIns(4,5)P2-dependent processes in Drosophila development.
Title: PI4KIII? is required for cortical integrity and cell polarity during Drosophila oogenesis.

Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling.
Title: Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling.

The following PI4KIIIalpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PI4KIIIalpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00619	NM_130658.3 Latest version!	Drosophila melanogaster phosphatidylinositol 4-kinase III alpha, transcript variant C (PI4KIIIalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa02771	NM_001272262.2 Latest version!	Drosophila melanogaster phosphatidylinositol 4-kinase III alpha, transcript variant D (PI4KIIIalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PI4KIIIalpha ( NM_130658.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_570014.3 Drosophila melanogaster phosphatidylinositol 4-kinase III alpha, transcript variant C (PI4KIIIalpha), mRNA.

Price & Availability↑

CloneID	OFa00619
Clone ID Related Accession (Same CDS sequence)	NM_130658.3
Accession Version	NM_130658.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatidylinositol 4-kinase III alpha, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_130658.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGA CCGCCTCGGA TAAATACACG TACCAGCGGA CCGTTCTCTG CCTGGCCCGC 
GTTCTGGCGG GCATCCAGCC CACTCCCTGG GATAAGGTGC AGACCCTGTT CCGGTATTGT 
CCGCAGGAGA ATGCTGCCGG AGTGTTTTGC CTGGACACGC GGGCCCAGGA TGCCGTAATT 
GCGCTGGGCA TTTATTTTCT GGAGGGCGGC TGCCAGCACG AGGGCCAGAT TGTGCCCTAT 
CTGCTGCGTC TGGCCAAGTG TCTGCCGAAG GCGGTCTGGA TTGACGATGC CCGGAGCAAC 
AAAGTGGAAC GCGTTCGCAT TCCGTCGGCG GAAAAGTTCA GCTTCTGCTT GAACACCCTG 
CTGTCCGACA TAGCGGCCAA GTGTCCGGAT TCGCGCGAGG AGATCATCCT GAATCAGGTG 
GAAACGCTGA GCGCGCTGGC CAACATAGTC AAGTCGAGCA GGGACAGCAG CTCCGCACCG 
CCGCCCATCA TCCTGTGCAA GGCCACGGTG CCGCTGCTCT TCGGCTTGGC GCGATCGATG 
GGTCGCTATG CCAGCAACGA TCCACCGCTG CTGTGCCGCA TTTTTCCGCC CGAGCTGCTG 
CCCATCCAGA AGGGCGGCGG CCGCGACGGC ACCGGCTCGA GCAGCAGTGC CAGCGGCACC 
TGCGGCGGCT CCTTCAGCAG CAGCGAACGC CTGGCGGCCA CGCATCACTT CCGACCCATC 
ATACCGCGCT CCATGTCCGG CAGTCTGGCG CAGGCCCAGA ACGCAAGCTA CGACGATGGA 
AGGCAGCGGT GCGCGGGCGG CAAGCCCAGC AAGCCATCGT TGCACAGCTA CTTTTCGGTG 
CCGTACGATC CGCGGACGCA CTTCTTCACG CGCTACGGCT CCAGTTTCAA CCAATTCCCG 
AACATGCGCG TCTGCGAATC GCCCACGAAA GGCGGCCCAC GACCACTGTA TCGTGTGCCT 
CCGTTTCCCA TCCAGCATCT ACAGACCATA TTCGCGGTGT CCAAGAAGCT GCTCACCAAA 
GACACCCTCG AACATCTCGA CGAGCAGGCA AGCGATATAT TTTCGCTGCA CCAAATCAAG 
GGCTATTGCT ACAAGAGCTT CTCGGAAACG TTGAACCTGG TCCTGGTGAC CCTGCTCAGG 
GAACTGCTGC AGCATCAGGT TGACCTGCCC ACGCCGTTCA CCAAGGATGT GCAGGAGTTC 
GTCAAGCGGC TCTTTCTCAA CGGCCAAACG GAGCTGCAGA ATAAACAGCA GGATCAGGAA 
CGCGAGCGGC GCGAGGAGAA CGGCATCGCG GTGGTCAACA AGTACAAGGT CAATGTGATG 
GCCAATGCGG CGTGTGTCGA TCTCCTGGTT TGGGCCATTC GCGACGAAAC GGAGGCGGAC 
AAGCTGTGCG GTCGACTGTC GCAGAAACTG AACCTGGAGC TCAGTCACAA GATCGTGATG 
GATCACATGC CGCTGCTGAT GGTTTGCCTG GAGGGACTGG GCAAGCTGGC CCACAAATTT 
CCCAACATTG CCGGCACCTC GATCTCGTAT CTGCGCGACT TCCTTGTGGC GCCCAGTCCC 
ATTCTGGGCA AGCTTCACGA CCATGCCATG CAATCGCTGG CCCAGCAGAA GAAGGAGAAG 
GAACTAACGC CCTTCAAGAT CGCGGTCCAG CACTCGGACT CGCGGACGGC GGTCGTCATC 
TACGGGGATA ATCAAAAGCC GCCCGGCAGC GGAACCGGTC GCAGTGGTCA TGCCGCATTC 
GAGTCACTGC GCGATGCGGC CATTGAGAAT CTATCGATCG CCCTGCGCGC TGCCCACACC 
CTCGATCAGT TCTGTGTGCC AGCTCTGGTG GCCAACGTCT CCAATCGACT CTTCACAGCC 
GAAAAGTCTG GCAGTGAATC GCAAGGCGAG TGCCACAAAT CCAACCTGGT CAGCTTGAAC 
ATCATCGTGA TGCTGGGCCA TGTGGCCGTG GCGCTCAAGG ATACCTCGAA GACGACACAG 
AATATCCTGC AGTTCTTTAT CCAGCGCTTC TGCAAGGTGC CATCGGAGCA GAACGCTCTG 
ATTGTGGATC AGCTTGGCTG CATGATAATC TCGCAGTGTG AAACGCATGT CTTCGATGAG 
ATCATGAAGA TGTTCTCGCG CGTCACCGTG CAATCGGCAT CGTTGGCGTA CACCTCGGAT 
CCGGAGCACA GGAAGCAATT CCATCACGTC TCGGATGCGG TGGTCAATGC ATTGGGCAAT 
ATTGCGGCCA ACATTCAAGG GGATGCCGAG ATGCTGGAGC TGCTGGGCAA GCTGCTGGAA 
CTGTTCGTCC AGATCGGTTT GGATGGCGAG CGCAGCTACG ACAATACACC GGGCGCCCAG 
AAGGCCAGCT CGCGTGCTGG CAATCTGGGC ATGCTGATAC CGGTGATTGC GGTACTGGTG 
CGCCGCCTGC CGCCGATCAA GAATCCCCGC CAGCGACTGC ACAAGCTATT CAAGGACTTC 
TGGGCCTACT GTGTCGTAAT GGGCTTCACC AACGCCCGCC TTTGGCCAGC GGACTGGTAC 
CAGGGTGTGC AGCAGATAGC GGCCAAATCG CCGCTACTCA TCTCGCAGAC AGCCCACAAA 
TCGGATATGC GGGAGCTCAA CTATACGCTG GCCATTAAGA GCGATTCGGT GAACGAGCTG 
CGTAGCCAGA TTCTGGTGCT TCTCGAGCAC TCCTCGGACA ACGTGGCGAC GGCGATCAAT 
AAGCTCTCGT TTGCGCAGTG CACGTACCTG TTAAGCGTTT ACTGGCTGGA GATGCTGCGC 
GTGGAGAATG CGGATGAGCC GAGTCTGGAG CCGATCATGA GCTACCTTTG CGACACGGCG 
CTGCAGAGGG ACAAGACGGG GATCTGGCAG TGCGTCAAGT GCGTTGCCGA CCAGGTCTTC 
GAGAAGTTCC GCAATGTTCT ATACGCCCAC GATGAGATTC GCGAAAAGGT GCTGGAGTCG 
CAAGCCACGC TGCTGCTGGT CTACTTCAAT CACATCCACA AACCCATCCA GATGGTGGCC 
GATCAGTACC TCTCCTTTCT GGTGGATCGC TTTCCCCACT TGCTTTGGAA TCGCCGCGTG 
CTGTGGTGCA TGCTGGACAT CCTCCAGCTG CTGGCATACT CACTCAGCCT GGATCCCAAC 
GAGGAGACGC CCACGTTGCG TGTAGTCTCG ACGCCCTACA CACTGCAGCT GATGGACTCG 
CTGCCGGCGC GAGAGCTGCG CTTGAAGGAC TTCGCCGATC GTTGCCAGGG CATTGTGAAT 
GAAGCCATGA AATGGGCGCC GCGATCCACA CGCTCGCACC TACAGGAGTA TCCCAATCAG 
ATTCCCACGC CCGTGCTGGC TCACCACAGC GGATTGGCGC TGGCATTCGA CTCGGTGGTG 
AGCAGTAGTG CCCAGCACAC GGGCACCATG AGCAAGCGGC CGAGCTGTGT CAACTCCGAC 
ACGCCCAGAT TCGTATCGGT TTTGTGCCTG AGAAGCAAAT ACGCCGGTGA GATCAGTGGC 
TTGCTGTCCG TACTTAGCGA GAAGGATAAG GCCGGCTTGG CGGACCGACT GGTCAGCGAT 
GTGTGGGAGG CGTGTGCCGA GAAGAGCGAC GCCCGCCATC GTGGCGCTCT ATGGCGTGCC 
ACCGCCTATC TGATCATATG CTCGGAGATC AGCAGGAAAC TGCTGCATGC GGTGGCCAGT 
TCGCAGCTGG AGCTGTTCAC CGAATCGGCG ATGGAGACCG CCGTTGAGTG CTGGCAGTGG 
GTGCTGACTG CGCGCCAGGA TCTGGAGCTG TGCTTCATCC AGGAGATGGT CAGCGCCTGG 
CAGACGACAT TCGAGAAGCG AATGGGCTTG TTTGCGTGGG AAACGGAGGT CACCCATCCA 
CTGGCCGCCT ACGAAGGCTG CAAGCTGGTC AGCAAGCCCA TACTGATCGC GCCCCATTTG 
ATTTGGCTGC AGCTGCTCTC CGAAATGGTG GACACAGCCA AGTATTGCAA CAGGGACAAG 
GTGGAGATGT TCTGCCTCCT GTTGCACCGA TGTCTTCCCG TTCTCAAGAG TAGCAAACAG 
AACCGTCAGG TGTCCACCGT TGGCTGCCGC TTCAAGCTGC TGCAGTGCGG TCTGTCGCTG 
CTGCAGGGCA ACACCATTCC GAAGTCGCTC TCCAGAAATA TCCTCCGCGA GCGGATATAC 
TCCAACGCAC TGGACTACTT TTGCGGACCA CCAACGTGCC CCAATCAGAG CAGGGAGCAG 
CTACTGGAGG ACATAATGAT ATTGCTGAAG TTCTGGCAGA CGATGCGCAG TGAAAAGAAG 
CATCTGGTGA CGTCGGAAGT GGGTGACTAT GACTTGACAA ATGCCTCCGT TAGCTCCACG 
CAAATGCTGG CCGTGAGAAA TAATCCGGAA ACGGCATCCC TGATCAGCGG CGGTGGGCTA 
GTCAACGATT ACACGCGCTC CATGAGTGCC AGTGGCAATG CGGTGGGCAT GGGCATGGGT 
GTAGCGGGCG GCGGAAGCAG CAGTGGCTGG TACAACACCA TTCCGCACTC CACATCCACA 
CTGTCGAAGC GATCGAATCG CTCCAAGCGG CTGCAGTATC AGAAGGATTC CTACGACAAG 
GACTACATGA AGAAGCGCAA TCTGATCCTT GAGCTGCTGG CCGTCGAGCT CGAGTTCCTC 
ATTACCTGGT ACAATCCCAA TAGTCTGCCG GATCTTATAG TGCCGGGCGA GGAACAGATC 
ACGGAATGGC GCAACCGGCC GTACAAGTCG ACCGTTTGGC GGGACTATGC GCGTCTCGCC 
TGGTGCTATA ACCCGGCACT GGCTGTCTTC CTACCGCAGC GGATCAAGAA CGCGGAGATC 
ATTGATGAGG AGGTGTCGCG GCTGGTCTGC TCCGATCCCA TAGCGGTGTG CCACATTCCC 
GAGGCGCTCA AATACCTGTG CACCACCAAG AACCTGCTGC AGGAGAGCCC GGACCTGGTG 
TACATACTCT CGTGGTCGCC AGTGACGCCC ATCCAGGCCC TGGCCTACTT CTCACGGCAG 
TATCCATCGC ATCCGCTAAC AGCTCAGTAT GCCGTAAAGA CCCTCTCCTC GTATCCGGCG 
GAATCGGTGC TGCCGTACAT CCCGCAACTG GTCCAAGCCC TTCGGCACGA CACCATGGGC 
TATGTGGTGG AGTTCATTAA GAATATATCG CGACGGTCGC AGATAGTGGC ACACCAATTG 
ATCTGGAACA TGCAGACGAA CATGTACATG GACGAGGATC AACAGCACAA GGATCCCAAT 
CTGTACGAGG CACTCGATCA GCTATCGCAA AGTATTATCG CATCGTTCTC GGGCGCTGCC 
AAGCGGTTCT ACGAGCGGGA ATTCGACTTC TTCGGCAAGA TAACGGCCGT ATCCGGCGAA 
ATTCGCTCGT TTGCCAAGGG CATTGAGCGG AAGAACGCAT GCCTGGCGGC GCTTAGCAGG 
ATCAAGGTGC AGGGCGGCTG TTATCTGCCC TCCAATCCGG AGGCCATGGT CCTCGATATC 
GACTACAGCA GCGGCACGCC GATGCAAAGT GCGGCCAAGG CGCCGTATTT GGCACGATTC 
CGTGTGTATC GTTGCGGCAT CACGGAGCTG GAGACGCGCG CCATGGAGGT GTCCAATAAT 
CCGAACTCCC AGGAGGATGC CAAGATGACG CTGGGCGTGG AGTCGTGGCA GGCGGCCATT 
TTCAAGGTGG GCGACGATGT GCGCCAGGAC ATGCTGGCGC TCCAGGTGAT CACCATCTTC 
AAGAACATCT TCCAGCAGGT GGGACTCGAT CTGTTCCTGT TCCCCTACCG CGTTGTGGCC 
ACCGCTCCAG GCTGCGGCGT CATCGAGTGC GTGCCGAATG CCAAGTCGAG GGATCAACTC 
GGCCGACAGA CGGACAGTGG ACTCTCCGAG TATTTCCAGC ATCAGTACGG CGACGAGAGC 
TCCAAGGAGT TCCAGGCGGC GCGCGCCAAC TTTGTCAAGT CCATGGCAGC CTACTCGTTG 
ATCGGCTATC TGCTCCAGAT TAAGGATCGC CACAATGGTA ACATCATGAT CGACAAGGAT 
GGCCACATCA TACACATAGA TTTTGGCTTT ATGTTCGAGT CGTCGCCAGG TGGTAATATC 
GGCTTCGAGC CGGACATGAA GCTGACCGAC GAAATGGTCA TGATCATGGG CGGCAAGATG 
GACTCGCCGG CCTTCAAGTG GTTCTGCGAG CTGTGCGTGC AGGCCTTCCT CGCCGTTCGT 
CCGTATCAGG ATGCAATTGT CTCGCTGGTA TCCCTGATGC TGGACACTGG ACTGCCCTGC 
TTCCGCGGCC AGACGATCAA TCTGCTGAAG CAGCGCTTCG TGGCCACCAA GAACAACAAG 
GAGGCGGCCG CCCACATGCT GGCTGTCATC CGCAATTCGT ACCAGAACTT CCGCACGCGT 
ACCTACGACA TGATCCAGTA CTACCAGAAC CAGATACCCT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570014.3
CDS	215..6619
Translation	MTMTASDKYTYQRTVLCLARVLAGIQPTPWDKVQTLFRYCPQENAAGVFCLDTRAQDAVIALGIYFLEGGCQHEGQIVPYLLRLAKCLPKAVWIDDARSNKVERVRIPSAEKFSFCLNTLLSDIAAKCPDSREEIILNQVETLSALANIVKSSRDSSSAPPPIILCKATVPLLFGLARSMGRYASNDPPLLCRIFPPELLPIQKGGGRDGTGSSSSASGTCGGSFSSSERLAATHHFRPIIPRSMSGSLAQAQNASYDDGRQRCAGGKPSKPSLHSYFSVPYDPRTHFFTRYGSSFNQFPNMRVCESPTKGGPRPLYRVPPFPIQHLQTIFAVSKKLLTKDTLEHLDEQASDIFSLHQIKGYCYKSFSETLNLVLVTLLRELLQHQVDLPTPFTKDVQEFVKRLFLNGQTELQNKQQDQERERREENGIAVVNKYKVNVMANAACVDLLVWAIRDETEADKLCGRLSQKLNLELSHKIVMDHMPLLMVCLEGLGKLAHKFPNIAGTSISYLRDFLVAPSPILGKLHDHAMQSLAQQKKEKELTPFKIAVQHSDSRTAVVIYGDNQKPPGSGTGRSGHAAFESLRDAAIENLSIALRAAHTLDQFCVPALVANVSNRLFTAEKSGSESQGECHKSNLVSLNIIVMLGHVAVALKDTSKTTQNILQFFIQRFCKVPSEQNALIVDQLGCMIISQCETHVFDEIMKMFSRVTVQSASLAYTSDPEHRKQFHHVSDAVVNALGNIAANIQGDAEMLELLGKLLELFVQIGLDGERSYDNTPGAQKASSRAGNLGMLIPVIAVLVRRLPPIKNPRQRLHKLFKDFWAYCVVMGFTNARLWPADWYQGVQQIAAKSPLLISQTAHKSDMRELNYTLAIKSDSVNELRSQILVLLEHSSDNVATAINKLSFAQCTYLLSVYWLEMLRVENADEPSLEPIMSYLCDTALQRDKTGIWQCVKCVADQVFEKFRNVLYAHDEIREKVLESQATLLLVYFNHIHKPIQMVADQYLSFLVDRFPHLLWNRRVLWCMLDILQLLAYSLSLDPNEETPTLRVVSTPYTLQLMDSLPARELRLKDFADRCQGIVNEAMKWAPRSTRSHLQEYPNQIPTPVLAHHSGLALAFDSVVSSSAQHTGTMSKRPSCVNSDTPRFVSVLCLRSKYAGEISGLLSVLSEKDKAGLADRLVSDVWEACAEKSDARHRGALWRATAYLIICSEISRKLLHAVASSQLELFTESAMETAVECWQWVLTARQDLELCFIQEMVSAWQTTFEKRMGLFAWETEVTHPLAAYEGCKLVSKPILIAPHLIWLQLLSEMVDTAKYCNRDKVEMFCLLLHRCLPVLKSSKQNRQVSTVGCRFKLLQCGLSLLQGNTIPKSLSRNILRERIYSNALDYFCGPPTCPNQSREQLLEDIMILLKFWQTMRSEKKHLVTSEVGDYDLTNASVSSTQMLAVRNNPETASLISGGGLVNDYTRSMSASGNAVGMGMGVAGGGSSSGWYNTIPHSTSTLSKRSNRSKRLQYQKDSYDKDYMKKRNLILELLAVELEFLITWYNPNSLPDLIVPGEEQITEWRNRPYKSTVWRDYARLAWCYNPALAVFLPQRIKNAEIIDEEVSRLVCSDPIAVCHIPEALKYLCTTKNLLQESPDLVYILSWSPVTPIQALAYFSRQYPSHPLTAQYAVKTLSSYPAESVLPYIPQLVQALRHDTMGYVVEFIKNISRRSQIVAHQLIWNMQTNMYMDEDQQHKDPNLYEALDQLSQSIIASFSGAAKRFYEREFDFFGKITAVSGEIRSFAKGIERKNACLAALSRIKVQGGCYLPSNPEAMVLDIDYSSGTPMQSAAKAPYLARFRVYRCGITELETRAMEVSNNPNSQEDAKMTLGVESWQAAIFKVGDDVRQDMLALQVITIFKNIFQQVGLDLFLFPYRVVATAPGCGVIECVPNAKSRDQLGRQTDSGLSEYFQHQYGDESSKEFQAARANFVKSMAAYSLIGYLLQIKDRHNGNIMIDKDGHIIHIDFGFMFESSPGGNIGFEPDMKLTDEMVMIMGGKMDSPAFKWFCELCVQAFLAVRPYQDAIVSLVSLMLDTGLPCFRGQTINLLKQRFVATKNNKEAAAHMLAVIRNSYQNFRTRTYDMIQYYQNQIPY

Target ORF information:

RefSeq Version	NM_130658.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatidylinositol 4-kinase III alpha, transcript variant C (PI4KIIIalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130658.3

ORF Insert Sequence:

ATGACGATGA CCGCCTCGGA TAAATACACG TACCAGCGGA CCGTTCTCTG CCTGGCCCGC 
GTTCTGGCGG GCATCCAGCC CACTCCCTGG GATAAGGTGC AGACCCTGTT CCGGTATTGT 
CCGCAGGAGA ATGCTGCCGG AGTGTTTTGC CTGGACACGC GGGCCCAGGA TGCCGTAATT 
GCGCTGGGCA TTTATTTTCT GGAGGGCGGC TGCCAGCACG AGGGCCAGAT TGTGCCCTAT 
CTGCTGCGTC TGGCCAAGTG TCTGCCGAAG GCGGTCTGGA TTGACGATGC CCGGAGCAAC 
AAAGTGGAAC GCGTTCGCAT TCCGTCGGCG GAAAAGTTCA GCTTCTGCTT GAACACCCTG 
CTGTCCGACA TAGCGGCCAA GTGTCCGGAT TCGCGCGAGG AGATCATCCT GAATCAGGTG 
GAAACGCTGA GCGCGCTGGC CAACATAGTC AAGTCGAGCA GGGACAGCAG CTCCGCACCG 
CCGCCCATCA TCCTGTGCAA GGCCACGGTG CCGCTGCTCT TCGGCTTGGC GCGATCGATG 
GGTCGCTATG CCAGCAACGA TCCACCGCTG CTGTGCCGCA TTTTTCCGCC CGAGCTGCTG 
CCCATCCAGA AGGGCGGCGG CCGCGACGGC ACCGGCTCGA GCAGCAGTGC CAGCGGCACC 
TGCGGCGGCT CCTTCAGCAG CAGCGAACGC CTGGCGGCCA CGCATCACTT CCGACCCATC 
ATACCGCGCT CCATGTCCGG CAGTCTGGCG CAGGCCCAGA ACGCAAGCTA CGACGATGGA 
AGGCAGCGGT GCGCGGGCGG CAAGCCCAGC AAGCCATCGT TGCACAGCTA CTTTTCGGTG 
CCGTACGATC CGCGGACGCA CTTCTTCACG CGCTACGGCT CCAGTTTCAA CCAATTCCCG 
AACATGCGCG TCTGCGAATC GCCCACGAAA GGCGGCCCAC GACCACTGTA TCGTGTGCCT 
CCGTTTCCCA TCCAGCATCT ACAGACCATA TTCGCGGTGT CCAAGAAGCT GCTCACCAAA 
GACACCCTCG AACATCTCGA CGAGCAGGCA AGCGATATAT TTTCGCTGCA CCAAATCAAG 
GGCTATTGCT ACAAGAGCTT CTCGGAAACG TTGAACCTGG TCCTGGTGAC CCTGCTCAGG 
GAACTGCTGC AGCATCAGGT TGACCTGCCC ACGCCGTTCA CCAAGGATGT GCAGGAGTTC 
GTCAAGCGGC TCTTTCTCAA CGGCCAAACG GAGCTGCAGA ATAAACAGCA GGATCAGGAA 
CGCGAGCGGC GCGAGGAGAA CGGCATCGCG GTGGTCAACA AGTACAAGGT CAATGTGATG 
GCCAATGCGG CGTGTGTCGA TCTCCTGGTT TGGGCCATTC GCGACGAAAC GGAGGCGGAC 
AAGCTGTGCG GTCGACTGTC GCAGAAACTG AACCTGGAGC TCAGTCACAA GATCGTGATG 
GATCACATGC CGCTGCTGAT GGTTTGCCTG GAGGGACTGG GCAAGCTGGC CCACAAATTT 
CCCAACATTG CCGGCACCTC GATCTCGTAT CTGCGCGACT TCCTTGTGGC GCCCAGTCCC 
ATTCTGGGCA AGCTTCACGA CCATGCCATG CAATCGCTGG CCCAGCAGAA GAAGGAGAAG 
GAACTAACGC CCTTCAAGAT CGCGGTCCAG CACTCGGACT CGCGGACGGC GGTCGTCATC 
TACGGGGATA ATCAAAAGCC GCCCGGCAGC GGAACCGGTC GCAGTGGTCA TGCCGCATTC 
GAGTCACTGC GCGATGCGGC CATTGAGAAT CTATCGATCG CCCTGCGCGC TGCCCACACC 
CTCGATCAGT TCTGTGTGCC AGCTCTGGTG GCCAACGTCT CCAATCGACT CTTCACAGCC 
GAAAAGTCTG GCAGTGAATC GCAAGGCGAG TGCCACAAAT CCAACCTGGT CAGCTTGAAC 
ATCATCGTGA TGCTGGGCCA TGTGGCCGTG GCGCTCAAGG ATACCTCGAA GACGACACAG 
AATATCCTGC AGTTCTTTAT CCAGCGCTTC TGCAAGGTGC CATCGGAGCA GAACGCTCTG 
ATTGTGGATC AGCTTGGCTG CATGATAATC TCGCAGTGTG AAACGCATGT CTTCGATGAG 
ATCATGAAGA TGTTCTCGCG CGTCACCGTG CAATCGGCAT CGTTGGCGTA CACCTCGGAT 
CCGGAGCACA GGAAGCAATT CCATCACGTC TCGGATGCGG TGGTCAATGC ATTGGGCAAT 
ATTGCGGCCA ACATTCAAGG GGATGCCGAG ATGCTGGAGC TGCTGGGCAA GCTGCTGGAA 
CTGTTCGTCC AGATCGGTTT GGATGGCGAG CGCAGCTACG ACAATACACC GGGCGCCCAG 
AAGGCCAGCT CGCGTGCTGG CAATCTGGGC ATGCTGATAC CGGTGATTGC GGTACTGGTG 
CGCCGCCTGC CGCCGATCAA GAATCCCCGC CAGCGACTGC ACAAGCTATT CAAGGACTTC 
TGGGCCTACT GTGTCGTAAT GGGCTTCACC AACGCCCGCC TTTGGCCAGC GGACTGGTAC 
CAGGGTGTGC AGCAGATAGC GGCCAAATCG CCGCTACTCA TCTCGCAGAC AGCCCACAAA 
TCGGATATGC GGGAGCTCAA CTATACGCTG GCCATTAAGA GCGATTCGGT GAACGAGCTG 
CGTAGCCAGA TTCTGGTGCT TCTCGAGCAC TCCTCGGACA ACGTGGCGAC GGCGATCAAT 
AAGCTCTCGT TTGCGCAGTG CACGTACCTG TTAAGCGTTT ACTGGCTGGA GATGCTGCGC 
GTGGAGAATG CGGATGAGCC GAGTCTGGAG CCGATCATGA GCTACCTTTG CGACACGGCG 
CTGCAGAGGG ACAAGACGGG GATCTGGCAG TGCGTCAAGT GCGTTGCCGA CCAGGTCTTC 
GAGAAGTTCC GCAATGTTCT ATACGCCCAC GATGAGATTC GCGAAAAGGT GCTGGAGTCG 
CAAGCCACGC TGCTGCTGGT CTACTTCAAT CACATCCACA AACCCATCCA GATGGTGGCC 
GATCAGTACC TCTCCTTTCT GGTGGATCGC TTTCCCCACT TGCTTTGGAA TCGCCGCGTG 
CTGTGGTGCA TGCTGGACAT CCTCCAGCTG CTGGCATACT CACTCAGCCT GGATCCCAAC 
GAGGAGACGC CCACGTTGCG TGTAGTCTCG ACGCCCTACA CACTGCAGCT GATGGACTCG 
CTGCCGGCGC GAGAGCTGCG CTTGAAGGAC TTCGCCGATC GTTGCCAGGG CATTGTGAAT 
GAAGCCATGA AATGGGCGCC GCGATCCACA CGCTCGCACC TACAGGAGTA TCCCAATCAG 
ATTCCCACGC CCGTGCTGGC TCACCACAGC GGATTGGCGC TGGCATTCGA CTCGGTGGTG 
AGCAGTAGTG CCCAGCACAC GGGCACCATG AGCAAGCGGC CGAGCTGTGT CAACTCCGAC 
ACGCCCAGAT TCGTATCGGT TTTGTGCCTG AGAAGCAAAT ACGCCGGTGA GATCAGTGGC 
TTGCTGTCCG TACTTAGCGA GAAGGATAAG GCCGGCTTGG CGGACCGACT GGTCAGCGAT 
GTGTGGGAGG CGTGTGCCGA GAAGAGCGAC GCCCGCCATC GTGGCGCTCT ATGGCGTGCC 
ACCGCCTATC TGATCATATG CTCGGAGATC AGCAGGAAAC TGCTGCATGC GGTGGCCAGT 
TCGCAGCTGG AGCTGTTCAC CGAATCGGCG ATGGAGACCG CCGTTGAGTG CTGGCAGTGG 
GTGCTGACTG CGCGCCAGGA TCTGGAGCTG TGCTTCATCC AGGAGATGGT CAGCGCCTGG 
CAGACGACAT TCGAGAAGCG AATGGGCTTG TTTGCGTGGG AAACGGAGGT CACCCATCCA 
CTGGCCGCCT ACGAAGGCTG CAAGCTGGTC AGCAAGCCCA TACTGATCGC GCCCCATTTG 
ATTTGGCTGC AGCTGCTCTC CGAAATGGTG GACACAGCCA AGTATTGCAA CAGGGACAAG 
GTGGAGATGT TCTGCCTCCT GTTGCACCGA TGTCTTCCCG TTCTCAAGAG TAGCAAACAG 
AACCGTCAGG TGTCCACCGT TGGCTGCCGC TTCAAGCTGC TGCAGTGCGG TCTGTCGCTG 
CTGCAGGGCA ACACCATTCC GAAGTCGCTC TCCAGAAATA TCCTCCGCGA GCGGATATAC 
TCCAACGCAC TGGACTACTT TTGCGGACCA CCAACGTGCC CCAATCAGAG CAGGGAGCAG 
CTACTGGAGG ACATAATGAT ATTGCTGAAG TTCTGGCAGA CGATGCGCAG TGAAAAGAAG 
CATCTGGTGA CGTCGGAAGT GGGTGACTAT GACTTGACAA ATGCCTCCGT TAGCTCCACG 
CAAATGCTGG CCGTGAGAAA TAATCCGGAA ACGGCATCCC TGATCAGCGG CGGTGGGCTA 
GTCAACGATT ACACGCGCTC CATGAGTGCC AGTGGCAATG CGGTGGGCAT GGGCATGGGT 
GTAGCGGGCG GCGGAAGCAG CAGTGGCTGG TACAACACCA TTCCGCACTC CACATCCACA 
CTGTCGAAGC GATCGAATCG CTCCAAGCGG CTGCAGTATC AGAAGGATTC CTACGACAAG 
GACTACATGA AGAAGCGCAA TCTGATCCTT GAGCTGCTGG CCGTCGAGCT CGAGTTCCTC 
ATTACCTGGT ACAATCCCAA TAGTCTGCCG GATCTTATAG TGCCGGGCGA GGAACAGATC 
ACGGAATGGC GCAACCGGCC GTACAAGTCG ACCGTTTGGC GGGACTATGC GCGTCTCGCC 
TGGTGCTATA ACCCGGCACT GGCTGTCTTC CTACCGCAGC GGATCAAGAA CGCGGAGATC 
ATTGATGAGG AGGTGTCGCG GCTGGTCTGC TCCGATCCCA TAGCGGTGTG CCACATTCCC 
GAGGCGCTCA AATACCTGTG CACCACCAAG AACCTGCTGC AGGAGAGCCC GGACCTGGTG 
TACATACTCT CGTGGTCGCC AGTGACGCCC ATCCAGGCCC TGGCCTACTT CTCACGGCAG 
TATCCATCGC ATCCGCTAAC AGCTCAGTAT GCCGTAAAGA CCCTCTCCTC GTATCCGGCG 
GAATCGGTGC TGCCGTACAT CCCGCAACTG GTCCAAGCCC TTCGGCACGA CACCATGGGC 
TATGTGGTGG AGTTCATTAA GAATATATCG CGACGGTCGC AGATAGTGGC ACACCAATTG 
ATCTGGAACA TGCAGACGAA CATGTACATG GACGAGGATC AACAGCACAA GGATCCCAAT 
CTGTACGAGG CACTCGATCA GCTATCGCAA AGTATTATCG CATCGTTCTC GGGCGCTGCC 
AAGCGGTTCT ACGAGCGGGA ATTCGACTTC TTCGGCAAGA TAACGGCCGT ATCCGGCGAA 
ATTCGCTCGT TTGCCAAGGG CATTGAGCGG AAGAACGCAT GCCTGGCGGC GCTTAGCAGG 
ATCAAGGTGC AGGGCGGCTG TTATCTGCCC TCCAATCCGG AGGCCATGGT CCTCGATATC 
GACTACAGCA GCGGCACGCC GATGCAAAGT GCGGCCAAGG CGCCGTATTT GGCACGATTC 
CGTGTGTATC GTTGCGGCAT CACGGAGCTG GAGACGCGCG CCATGGAGGT GTCCAATAAT 
CCGAACTCCC AGGAGGATGC CAAGATGACG CTGGGCGTGG AGTCGTGGCA GGCGGCCATT 
TTCAAGGTGG GCGACGATGT GCGCCAGGAC ATGCTGGCGC TCCAGGTGAT CACCATCTTC 
AAGAACATCT TCCAGCAGGT GGGACTCGAT CTGTTCCTGT TCCCCTACCG CGTTGTGGCC 
ACCGCTCCAG GCTGCGGCGT CATCGAGTGC GTGCCGAATG CCAAGTCGAG GGATCAACTC 
GGCCGACAGA CGGACAGTGG ACTCTCCGAG TATTTCCAGC ATCAGTACGG CGACGAGAGC 
TCCAAGGAGT TCCAGGCGGC GCGCGCCAAC TTTGTCAAGT CCATGGCAGC CTACTCGTTG 
ATCGGCTATC TGCTCCAGAT TAAGGATCGC CACAATGGTA ACATCATGAT CGACAAGGAT 
GGCCACATCA TACACATAGA TTTTGGCTTT ATGTTCGAGT CGTCGCCAGG TGGTAATATC 
GGCTTCGAGC CGGACATGAA GCTGACCGAC GAAATGGTCA TGATCATGGG CGGCAAGATG 
GACTCGCCGG CCTTCAAGTG GTTCTGCGAG CTGTGCGTGC AGGCCTTCCT CGCCGTTCGT 
CCGTATCAGG ATGCAATTGT CTCGCTGGTA TCCCTGATGC TGGACACTGG ACTGCCCTGC 
TTCCGCGGCC AGACGATCAA TCTGCTGAAG CAGCGCTTCG TGGCCACCAA GAACAACAAG 
GAGGCGGCCG CCCACATGCT GGCTGTCATC CGCAATTCGT ACCAGAACTT CCGCACGCGT 
ACCTACGACA TGATCCAGTA CTACCAGAAC CAGATACCCT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI4KIIIalpha ( NM_001272262.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259191.1 Drosophila melanogaster phosphatidylinositol 4-kinase III alpha, transcript variant D (PI4KIIIalpha), mRNA.

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CloneID	OFa02771
Clone ID Related Accession (Same CDS sequence)	NM_001272262.2
Accession Version	NM_001272262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatidylinositol 4-kinase III alpha, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272262.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGA CCGCCTCGGA TAAATACACG TACCAGCGGA CCGTTCTCTG CCTGGCCCGC 
GTTCTGGCGG GCATCCAGCC CACTCCCTGG GATAAGGTGC AGACCCTGTT CCGGTATTGT 
CCGCAGGAGA ATGCTGCCGG AGTGTTTTGC CTGGACACGC GGGCCCAGGA TGCCGTAATT 
GCGCTGGGCA TTTATTTTCT GGAGGGCGGC TGCCAGCACG AGGGCCAGAT TGTGCCCTAT 
CTGCTGCGTC TGGCCAAGTG TCTGCCGAAG GCGGTCTGGA TTGACGATGC CCGGAGCAAC 
AAAGTGGAAC GCGTTCGCAT TCCGTCGGCG GAAAAGTTCA GCTTCTGCTT GAACACCCTG 
CTGTCCGACA TAGCGGCCAA GTGTCCGGAT TCGCGCGAGG AGATCATCCT GAATCAGGTG 
GAAACGCTGA GCGCGCTGGC CAACATAGTC AAGTCGAGCA GGGACAGCAG CTCCGCACCG 
CCGCCCATCA TCCTGTGCAA GGCCACGGTG CCGCTGCTCT TCGGCTTGGC GCGATCGATG 
GGTCGCTATG CCAGCAACGA TCCACCGCTG CTGTGCCGCA TTTTTCCGCC CGAGCTGCTG 
CCCATCCAGA AGGGCGGCGG CCGCGACGGC ACCGGCTCGA GCAGCAGTGC CAGCGGCACC 
TGCGGCGGCT CCTTCAGCAG CAGCGAACGC CTGGCGGCCA CGCATCACTT CCGACCCATC 
ATACCGCGCT CCATGTCCGG CAGTCTGGCG CAGGCCCAGA ACGCAAGCTA CGACGATGGA 
AGGCAGCGGT GCGCGGGCGG CAAGCCCAGC AAGCCATCGT TGCACAGCTA CTTTTCGGTG 
CCGTACGATC CGCGGACGCA CTTCTTCACG CGCTACGGCT CCAGTTTCAA CCAATTCCCG 
AACATGCGCG TCTGCGAATC GCCCACGAAA GGCGGCCCAC GACCACTGTA TCGTGTGCCT 
CCGTTTCCCA TCCAGCATCT ACAGACCATA TTCGCGGTGT CCAAGAAGCT GCTCACCAAA 
GACACCCTCG AACATCTCGA CGAGCAGGCA AGCGATATAT TTTCGCTGCA CCAAATCAAG 
GGCTATTGCT ACAAGAGCTT CTCGGAAACG TTGAACCTGG TCCTGGTGAC CCTGCTCAGG 
GAACTGCTGC AGCATCAGGT TGACCTGCCC ACGCCGTTCA CCAAGGATGT GCAGGAGTTC 
GTCAAGCGGC TCTTTCTCAA CGGCCAAACG GAGCTGCAGA ATAAACAGCA GGATCAGGAA 
CGCGAGCGGC GCGAGGAGAA CGGCATCGCG GTGGTCAACA AGTACAAGGT CAATGTGATG 
GCCAATGCGG CGTGTGTCGA TCTCCTGGTT TGGGCCATTC GCGACGAAAC GGTTGACGTC 
GACTATAATG TGCCATCCCT GCAGAATGGC TTGCAATTTT TATTGAAGAA AGAGGCGGAC 
AAGCTGTGCG GTCGACTGTC GCAGAAACTG AACCTGGAGC TCAGTCACAA GATCGTGATG 
GATCACATGC CGCTGCTGAT GGTTTGCCTG GAGGGACTGG GCAAGCTGGC CCACAAATTT 
CCCAACATTG CCGGCACCTC GATCTCGTAT CTGCGCGACT TCCTTGTGGC GCCCAGTCCC 
ATTCTGGGCA AGCTTCACGA CCATGCCATG CAATCGCTGG CCCAGCAGAA GAAGGAGAAG 
GAACTAACGC CCTTCAAGAT CGCGGTCCAG CACTCGGACT CGCGGACGGC GGTCGTCATC 
TACGGGGATA ATCAAAAGCC GCCCGGCAGC GGAACCGGTC GCAGTGGTCA TGCCGCATTC 
GAGTCACTGC GCGATGCGGC CATTGAGAAT CTATCGATCG CCCTGCGCGC TGCCCACACC 
CTCGATCAGT TCTGTGTGCC AGCTCTGGTG GCCAACGTCT CCAATCGACT CTTCACAGCC 
GAAAAGTCTG GCAGTGAATC GCAAGGCGAG TGCCACAAAT CCAACCTGGT CAGCTTGAAC 
ATCATCGTGA TGCTGGGCCA TGTGGCCGTG GCGCTCAAGG ATACCTCGAA GACGACACAG 
AATATCCTGC AGTTCTTTAT CCAGCGCTTC TGCAAGGTGC CATCGGAGCA GAACGCTCTG 
ATTGTGGATC AGCTTGGCTG CATGATAATC TCGCAGTGTG AAACGCATGT CTTCGATGAG 
ATCATGAAGA TGTTCTCGCG CGTCACCGTG CAATCGGCAT CGTTGGCGTA CACCTCGGAT 
CCGGAGCACA GGAAGCAATT CCATCACGTC TCGGATGCGG TGGTCAATGC ATTGGGCAAT 
ATTGCGGCCA ACATTCAAGG GGATGCCGAG ATGCTGGAGC TGCTGGGCAA GCTGCTGGAA 
CTGTTCGTCC AGATCGGTTT GGATGGCGAG CGCAGCTACG ACAATACACC GGGCGCCCAG 
AAGGCCAGCT CGCGTGCTGG CAATCTGGGC ATGCTGATAC CGGTGATTGC GGTACTGGTG 
CGCCGCCTGC CGCCGATCAA GAATCCCCGC CAGCGACTGC ACAAGCTATT CAAGGACTTC 
TGGGCCTACT GTGTCGTAAT GGGCTTCACC AACGCCCGCC TTTGGCCAGC GGACTGGTAC 
CAGGGTGTGC AGCAGATAGC GGCCAAATCG CCGCTACTCA TCTCGCAGAC AGCCCACAAA 
TCGGATATGC GGGAGCTCAA CTATACGCTG GCCATTAAGA GCGATTCGGT GAACGAGCTG 
CGTAGCCAGA TTCTGGTGCT TCTCGAGCAC TCCTCGGACA ACGTGGCGAC GGCGATCAAT 
AAGCTCTCGT TTGCGCAGTG CACGTACCTG TTAAGCGTTT ACTGGCTGGA GATGCTGCGC 
GTGGAGAATG CGGATGAGCC GAGTCTGGAG CCGATCATGA GCTACCTTTG CGACACGGCG 
CTGCAGAGGG ACAAGACGGG GATCTGGCAG TGCGTCAAGT GCGTTGCCGA CCAGGTCTTC 
GAGAAGTTCC GCAATGTTCT ATACGCCCAC GATGAGATTC GCGAAAAGGT GCTGGAGTCG 
CAAGCCACGC TGCTGCTGGT CTACTTCAAT CACATCCACA AACCCATCCA GATGGTGGCC 
GATCAGTACC TCTCCTTTCT GGTGGATCGC TTTCCCCACT TGCTTTGGAA TCGCCGCGTG 
CTGTGGTGCA TGCTGGACAT CCTCCAGCTG CTGGCATACT CACTCAGCCT GGATCCCAAC 
GAGGAGACGC CCACGTTGCG TGTAGTCTCG ACGCCCTACA CACTGCAGCT GATGGACTCG 
CTGCCGGCGC GAGAGCTGCG CTTGAAGGAC TTCGCCGATC GTTGCCAGGG CATTGTGAAT 
GAAGCCATGA AATGGGCGCC GCGATCCACA CGCTCGCACC TACAGGAGTA TCCCAATCAG 
ATTCCCACGC CCGTGCTGGC TCACCACAGC GGATTGGCGC TGGCATTCGA CTCGGTGGTG 
AGCAGTAGTG CCCAGCACAC GGGCACCATG AGCAAGCGGC CGAGCTGTGT CAACTCCGAC 
ACGCCCAGAT TCGTATCGGT TTTGTGCCTG AGAAGCAAAT ACGCCGGTGA GATCAGTGGC 
TTGCTGTCCG TACTTAGCGA GAAGGATAAG GCCGGCTTGG CGGACCGACT GGTCAGCGAT 
GTGTGGGAGG CGTGTGCCGA GAAGAGCGAC GCCCGCCATC GTGGCGCTCT ATGGCGTGCC 
ACCGCCTATC TGATCATATG CTCGGAGATC AGCAGGAAAC TGCTGCATGC GGTGGCCAGT 
TCGCAGCTGG AGCTGTTCAC CGAATCGGCG ATGGAGACCG CCGTTGAGTG CTGGCAGTGG 
GTGCTGACTG CGCGCCAGGA TCTGGAGCTG TGCTTCATCC AGGAGATGGT CAGCGCCTGG 
CAGACGACAT TCGAGAAGCG AATGGGCTTG TTTGCGTGGG AAACGGAGGT CACCCATCCA 
CTGGCCGCCT ACGAAGGCTG CAAGCTGGTC AGCAAGCCCA TACTGATCGC GCCCCATTTG 
ATTTGGCTGC AGCTGCTCTC CGAAATGGTG GACACAGCCA AGTATTGCAA CAGGGACAAG 
GTGGAGATGT TCTGCCTCCT GTTGCACCGA TGTCTTCCCG TTCTCAAGAG TAGCAAACAG 
AACCGTCAGG TGTCCACCGT TGGCTGCCGC TTCAAGCTGC TGCAGTGCGG TCTGTCGCTG 
CTGCAGGGCA ACACCATTCC GAAGTCGCTC TCCAGAAATA TCCTCCGCGA GCGGATATAC 
TCCAACGCAC TGGACTACTT TTGCGGACCA CCAACGTGCC CCAATCAGAG CAGGGAGCAG 
CTACTGGAGG ACATAATGAT ATTGCTGAAG TTCTGGCAGA CGATGCGCAG TGAAAAGAAG 
CATCTGGTGA CGTCGGAAGT GGGTGACTAT GACTTGACAA ATGCCTCCGT TAGCTCCACG 
CAAATGCTGG CCGTGAGAAA TAATCCGGAA ACGGCATCCC TGATCAGCGG CGGTGGGCTA 
GTCAACGATT ACACGCGCTC CATGAGTGCC AGTGGCAATG CGGTGGGCAT GGGCATGGGT 
GTAGCGGGCG GCGGAAGCAG CAGTGGCTGG TACAACACCA TTCCGCACTC CACATCCACA 
CTGTCGAAGC GATCGAATCG CTCCAAGCGG CTGCAGTATC AGAAGGATTC CTACGACAAG 
GACTACATGA AGAAGCGCAA TCTGATCCTT GAGCTGCTGG CCGTCGAGCT CGAGTTCCTC 
ATTACCTGGT ACAATCCCAA TAGTCTGCCG GATCTTATAG TGCCGGGCGA GGAACAGATC 
ACGGAATGGC GCAACCGGCC GTACAAGTCG ACCGTTTGGC GGGACTATGC GCGTCTCGCC 
TGGTGCTATA ACCCGGCACT GGCTGTCTTC CTACCGCAGC GGATCAAGAA CGCGGAGATC 
ATTGATGAGG AGGTGTCGCG GCTGGTCTGC TCCGATCCCA TAGCGGTGTG CCACATTCCC 
GAGGCGCTCA AATACCTGTG CACCACCAAG AACCTGCTGC AGGAGAGCCC GGACCTGGTG 
TACATACTCT CGTGGTCGCC AGTGACGCCC ATCCAGGCCC TGGCCTACTT CTCACGGCAG 
TATCCATCGC ATCCGCTAAC AGCTCAGTAT GCCGTAAAGA CCCTCTCCTC GTATCCGGCG 
GAATCGGTGC TGCCGTACAT CCCGCAACTG GTCCAAGCCC TTCGGCACGA CACCATGGGC 
TATGTGGTGG AGTTCATTAA GAATATATCG CGACGGTCGC AGATAGTGGC ACACCAATTG 
ATCTGGAACA TGCAGACGAA CATGTACATG GACGAGGATC AACAGCACAA GGATCCCAAT 
CTGTACGAGG CACTCGATCA GCTATCGCAA AGTATTATCG CATCGTTCTC GGGCGCTGCC 
AAGCGGTTCT ACGAGCGGGA ATTCGACTTC TTCGGCAAGA TAACGGCCGT ATCCGGCGAA 
ATTCGCTCGT TTGCCAAGGG CATTGAGCGG AAGAACGCAT GCCTGGCGGC GCTTAGCAGG 
ATCAAGGTGC AGGGCGGCTG TTATCTGCCC TCCAATCCGG AGGCCATGGT CCTCGATATC 
GACTACAGCA GCGGCACGCC GATGCAAAGT GCGGCCAAGG CGCCGTATTT GGCACGATTC 
CGTGTGTATC GTTGCGGCAT CACGGAGCTG GAGACGCGCG CCATGGAGGT GTCCAATAAT 
CCGAACTCCC AGGAGGATGC CAAGATGACG CTGGGCGTGG AGTCGTGGCA GGCGGCCATT 
TTCAAGGTGG GCGACGATGT GCGCCAGGAC ATGCTGGCGC TCCAGGTGAT CACCATCTTC 
AAGAACATCT TCCAGCAGGT GGGACTCGAT CTGTTCCTGT TCCCCTACCG CGTTGTGGCC 
ACCGCTCCAG GCTGCGGCGT CATCGAGTGC GTGCCGAATG CCAAGTCGAG GGATCAACTC 
GGCCGACAGA CGGACAGTGG ACTCTCCGAG TATTTCCAGC ATCAGTACGG CGACGAGAGC 
TCCAAGGAGT TCCAGGCGGC GCGCGCCAAC TTTGTCAAGT CCATGGCAGC CTACTCGTTG 
ATCGGCTATC TGCTCCAGAT TAAGGATCGC CACAATGGTA ACATCATGAT CGACAAGGAT 
GGCCACATCA TACACATAGA TTTTGGCTTT ATGTTCGAGT CGTCGCCAGG TGGTAATATC 
GGCTTCGAGC CGGACATGAA GCTGACCGAC GAAATGGTCA TGATCATGGG CGGCAAGATG 
GACTCGCCGG CCTTCAAGTG GTTCTGCGAG CTGTGCGTGC AGGCCTTCCT CGCCGTTCGT 
CCGTATCAGG ATGCAATTGT CTCGCTGGTA TCCCTGATGC TGGACACTGG ACTGCCCTGC 
TTCCGCGGCC AGACGATCAA TCTGCTGAAG CAGCGCTTCG TGGCCACCAA GAACAACAAG 
GAGGCGGCCG CCCACATGCT GGCTGTCATC CGCAATTCGT ACCAGAACTT CCGCACGCGT 
ACCTACGACA TGATCCAGTA CTACCAGAAC CAGATACCCT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259191.1
CDS	215..6679
Translation	MTMTASDKYTYQRTVLCLARVLAGIQPTPWDKVQTLFRYCPQENAAGVFCLDTRAQDAVIALGIYFLEGGCQHEGQIVPYLLRLAKCLPKAVWIDDARSNKVERVRIPSAEKFSFCLNTLLSDIAAKCPDSREEIILNQVETLSALANIVKSSRDSSSAPPPIILCKATVPLLFGLARSMGRYASNDPPLLCRIFPPELLPIQKGGGRDGTGSSSSASGTCGGSFSSSERLAATHHFRPIIPRSMSGSLAQAQNASYDDGRQRCAGGKPSKPSLHSYFSVPYDPRTHFFTRYGSSFNQFPNMRVCESPTKGGPRPLYRVPPFPIQHLQTIFAVSKKLLTKDTLEHLDEQASDIFSLHQIKGYCYKSFSETLNLVLVTLLRELLQHQVDLPTPFTKDVQEFVKRLFLNGQTELQNKQQDQERERREENGIAVVNKYKVNVMANAACVDLLVWAIRDETVDVDYNVPSLQNGLQFLLKKEADKLCGRLSQKLNLELSHKIVMDHMPLLMVCLEGLGKLAHKFPNIAGTSISYLRDFLVAPSPILGKLHDHAMQSLAQQKKEKELTPFKIAVQHSDSRTAVVIYGDNQKPPGSGTGRSGHAAFESLRDAAIENLSIALRAAHTLDQFCVPALVANVSNRLFTAEKSGSESQGECHKSNLVSLNIIVMLGHVAVALKDTSKTTQNILQFFIQRFCKVPSEQNALIVDQLGCMIISQCETHVFDEIMKMFSRVTVQSASLAYTSDPEHRKQFHHVSDAVVNALGNIAANIQGDAEMLELLGKLLELFVQIGLDGERSYDNTPGAQKASSRAGNLGMLIPVIAVLVRRLPPIKNPRQRLHKLFKDFWAYCVVMGFTNARLWPADWYQGVQQIAAKSPLLISQTAHKSDMRELNYTLAIKSDSVNELRSQILVLLEHSSDNVATAINKLSFAQCTYLLSVYWLEMLRVENADEPSLEPIMSYLCDTALQRDKTGIWQCVKCVADQVFEKFRNVLYAHDEIREKVLESQATLLLVYFNHIHKPIQMVADQYLSFLVDRFPHLLWNRRVLWCMLDILQLLAYSLSLDPNEETPTLRVVSTPYTLQLMDSLPARELRLKDFADRCQGIVNEAMKWAPRSTRSHLQEYPNQIPTPVLAHHSGLALAFDSVVSSSAQHTGTMSKRPSCVNSDTPRFVSVLCLRSKYAGEISGLLSVLSEKDKAGLADRLVSDVWEACAEKSDARHRGALWRATAYLIICSEISRKLLHAVASSQLELFTESAMETAVECWQWVLTARQDLELCFIQEMVSAWQTTFEKRMGLFAWETEVTHPLAAYEGCKLVSKPILIAPHLIWLQLLSEMVDTAKYCNRDKVEMFCLLLHRCLPVLKSSKQNRQVSTVGCRFKLLQCGLSLLQGNTIPKSLSRNILRERIYSNALDYFCGPPTCPNQSREQLLEDIMILLKFWQTMRSEKKHLVTSEVGDYDLTNASVSSTQMLAVRNNPETASLISGGGLVNDYTRSMSASGNAVGMGMGVAGGGSSSGWYNTIPHSTSTLSKRSNRSKRLQYQKDSYDKDYMKKRNLILELLAVELEFLITWYNPNSLPDLIVPGEEQITEWRNRPYKSTVWRDYARLAWCYNPALAVFLPQRIKNAEIIDEEVSRLVCSDPIAVCHIPEALKYLCTTKNLLQESPDLVYILSWSPVTPIQALAYFSRQYPSHPLTAQYAVKTLSSYPAESVLPYIPQLVQALRHDTMGYVVEFIKNISRRSQIVAHQLIWNMQTNMYMDEDQQHKDPNLYEALDQLSQSIIASFSGAAKRFYEREFDFFGKITAVSGEIRSFAKGIERKNACLAALSRIKVQGGCYLPSNPEAMVLDIDYSSGTPMQSAAKAPYLARFRVYRCGITELETRAMEVSNNPNSQEDAKMTLGVESWQAAIFKVGDDVRQDMLALQVITIFKNIFQQVGLDLFLFPYRVVATAPGCGVIECVPNAKSRDQLGRQTDSGLSEYFQHQYGDESSKEFQAARANFVKSMAAYSLIGYLLQIKDRHNGNIMIDKDGHIIHIDFGFMFESSPGGNIGFEPDMKLTDEMVMIMGGKMDSPAFKWFCELCVQAFLAVRPYQDAIVSLVSLMLDTGLPCFRGQTINLLKQRFVATKNNKEAAAHMLAVIRNSYQNFRTRTYDMIQYYQNQIPY

Target ORF information:

RefSeq Version	NM_001272262.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatidylinositol 4-kinase III alpha, transcript variant D (PI4KIIIalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272262.2

ORF Insert Sequence:

ATGACGATGA CCGCCTCGGA TAAATACACG TACCAGCGGA CCGTTCTCTG CCTGGCCCGC 
GTTCTGGCGG GCATCCAGCC CACTCCCTGG GATAAGGTGC AGACCCTGTT CCGGTATTGT 
CCGCAGGAGA ATGCTGCCGG AGTGTTTTGC CTGGACACGC GGGCCCAGGA TGCCGTAATT 
GCGCTGGGCA TTTATTTTCT GGAGGGCGGC TGCCAGCACG AGGGCCAGAT TGTGCCCTAT 
CTGCTGCGTC TGGCCAAGTG TCTGCCGAAG GCGGTCTGGA TTGACGATGC CCGGAGCAAC 
AAAGTGGAAC GCGTTCGCAT TCCGTCGGCG GAAAAGTTCA GCTTCTGCTT GAACACCCTG 
CTGTCCGACA TAGCGGCCAA GTGTCCGGAT TCGCGCGAGG AGATCATCCT GAATCAGGTG 
GAAACGCTGA GCGCGCTGGC CAACATAGTC AAGTCGAGCA GGGACAGCAG CTCCGCACCG 
CCGCCCATCA TCCTGTGCAA GGCCACGGTG CCGCTGCTCT TCGGCTTGGC GCGATCGATG 
GGTCGCTATG CCAGCAACGA TCCACCGCTG CTGTGCCGCA TTTTTCCGCC CGAGCTGCTG 
CCCATCCAGA AGGGCGGCGG CCGCGACGGC ACCGGCTCGA GCAGCAGTGC CAGCGGCACC 
TGCGGCGGCT CCTTCAGCAG CAGCGAACGC CTGGCGGCCA CGCATCACTT CCGACCCATC 
ATACCGCGCT CCATGTCCGG CAGTCTGGCG CAGGCCCAGA ACGCAAGCTA CGACGATGGA 
AGGCAGCGGT GCGCGGGCGG CAAGCCCAGC AAGCCATCGT TGCACAGCTA CTTTTCGGTG 
CCGTACGATC CGCGGACGCA CTTCTTCACG CGCTACGGCT CCAGTTTCAA CCAATTCCCG 
AACATGCGCG TCTGCGAATC GCCCACGAAA GGCGGCCCAC GACCACTGTA TCGTGTGCCT 
CCGTTTCCCA TCCAGCATCT ACAGACCATA TTCGCGGTGT CCAAGAAGCT GCTCACCAAA 
GACACCCTCG AACATCTCGA CGAGCAGGCA AGCGATATAT TTTCGCTGCA CCAAATCAAG 
GGCTATTGCT ACAAGAGCTT CTCGGAAACG TTGAACCTGG TCCTGGTGAC CCTGCTCAGG 
GAACTGCTGC AGCATCAGGT TGACCTGCCC ACGCCGTTCA CCAAGGATGT GCAGGAGTTC 
GTCAAGCGGC TCTTTCTCAA CGGCCAAACG GAGCTGCAGA ATAAACAGCA GGATCAGGAA 
CGCGAGCGGC GCGAGGAGAA CGGCATCGCG GTGGTCAACA AGTACAAGGT CAATGTGATG 
GCCAATGCGG CGTGTGTCGA TCTCCTGGTT TGGGCCATTC GCGACGAAAC GGTTGACGTC 
GACTATAATG TGCCATCCCT GCAGAATGGC TTGCAATTTT TATTGAAGAA AGAGGCGGAC 
AAGCTGTGCG GTCGACTGTC GCAGAAACTG AACCTGGAGC TCAGTCACAA GATCGTGATG 
GATCACATGC CGCTGCTGAT GGTTTGCCTG GAGGGACTGG GCAAGCTGGC CCACAAATTT 
CCCAACATTG CCGGCACCTC GATCTCGTAT CTGCGCGACT TCCTTGTGGC GCCCAGTCCC 
ATTCTGGGCA AGCTTCACGA CCATGCCATG CAATCGCTGG CCCAGCAGAA GAAGGAGAAG 
GAACTAACGC CCTTCAAGAT CGCGGTCCAG CACTCGGACT CGCGGACGGC GGTCGTCATC 
TACGGGGATA ATCAAAAGCC GCCCGGCAGC GGAACCGGTC GCAGTGGTCA TGCCGCATTC 
GAGTCACTGC GCGATGCGGC CATTGAGAAT CTATCGATCG CCCTGCGCGC TGCCCACACC 
CTCGATCAGT TCTGTGTGCC AGCTCTGGTG GCCAACGTCT CCAATCGACT CTTCACAGCC 
GAAAAGTCTG GCAGTGAATC GCAAGGCGAG TGCCACAAAT CCAACCTGGT CAGCTTGAAC 
ATCATCGTGA TGCTGGGCCA TGTGGCCGTG GCGCTCAAGG ATACCTCGAA GACGACACAG 
AATATCCTGC AGTTCTTTAT CCAGCGCTTC TGCAAGGTGC CATCGGAGCA GAACGCTCTG 
ATTGTGGATC AGCTTGGCTG CATGATAATC TCGCAGTGTG AAACGCATGT CTTCGATGAG 
ATCATGAAGA TGTTCTCGCG CGTCACCGTG CAATCGGCAT CGTTGGCGTA CACCTCGGAT 
CCGGAGCACA GGAAGCAATT CCATCACGTC TCGGATGCGG TGGTCAATGC ATTGGGCAAT 
ATTGCGGCCA ACATTCAAGG GGATGCCGAG ATGCTGGAGC TGCTGGGCAA GCTGCTGGAA 
CTGTTCGTCC AGATCGGTTT GGATGGCGAG CGCAGCTACG ACAATACACC GGGCGCCCAG 
AAGGCCAGCT CGCGTGCTGG CAATCTGGGC ATGCTGATAC CGGTGATTGC GGTACTGGTG 
CGCCGCCTGC CGCCGATCAA GAATCCCCGC CAGCGACTGC ACAAGCTATT CAAGGACTTC 
TGGGCCTACT GTGTCGTAAT GGGCTTCACC AACGCCCGCC TTTGGCCAGC GGACTGGTAC 
CAGGGTGTGC AGCAGATAGC GGCCAAATCG CCGCTACTCA TCTCGCAGAC AGCCCACAAA 
TCGGATATGC GGGAGCTCAA CTATACGCTG GCCATTAAGA GCGATTCGGT GAACGAGCTG 
CGTAGCCAGA TTCTGGTGCT TCTCGAGCAC TCCTCGGACA ACGTGGCGAC GGCGATCAAT 
AAGCTCTCGT TTGCGCAGTG CACGTACCTG TTAAGCGTTT ACTGGCTGGA GATGCTGCGC 
GTGGAGAATG CGGATGAGCC GAGTCTGGAG CCGATCATGA GCTACCTTTG CGACACGGCG 
CTGCAGAGGG ACAAGACGGG GATCTGGCAG TGCGTCAAGT GCGTTGCCGA CCAGGTCTTC 
GAGAAGTTCC GCAATGTTCT ATACGCCCAC GATGAGATTC GCGAAAAGGT GCTGGAGTCG 
CAAGCCACGC TGCTGCTGGT CTACTTCAAT CACATCCACA AACCCATCCA GATGGTGGCC 
GATCAGTACC TCTCCTTTCT GGTGGATCGC TTTCCCCACT TGCTTTGGAA TCGCCGCGTG 
CTGTGGTGCA TGCTGGACAT CCTCCAGCTG CTGGCATACT CACTCAGCCT GGATCCCAAC 
GAGGAGACGC CCACGTTGCG TGTAGTCTCG ACGCCCTACA CACTGCAGCT GATGGACTCG 
CTGCCGGCGC GAGAGCTGCG CTTGAAGGAC TTCGCCGATC GTTGCCAGGG CATTGTGAAT 
GAAGCCATGA AATGGGCGCC GCGATCCACA CGCTCGCACC TACAGGAGTA TCCCAATCAG 
ATTCCCACGC CCGTGCTGGC TCACCACAGC GGATTGGCGC TGGCATTCGA CTCGGTGGTG 
AGCAGTAGTG CCCAGCACAC GGGCACCATG AGCAAGCGGC CGAGCTGTGT CAACTCCGAC 
ACGCCCAGAT TCGTATCGGT TTTGTGCCTG AGAAGCAAAT ACGCCGGTGA GATCAGTGGC 
TTGCTGTCCG TACTTAGCGA GAAGGATAAG GCCGGCTTGG CGGACCGACT GGTCAGCGAT 
GTGTGGGAGG CGTGTGCCGA GAAGAGCGAC GCCCGCCATC GTGGCGCTCT ATGGCGTGCC 
ACCGCCTATC TGATCATATG CTCGGAGATC AGCAGGAAAC TGCTGCATGC GGTGGCCAGT 
TCGCAGCTGG AGCTGTTCAC CGAATCGGCG ATGGAGACCG CCGTTGAGTG CTGGCAGTGG 
GTGCTGACTG CGCGCCAGGA TCTGGAGCTG TGCTTCATCC AGGAGATGGT CAGCGCCTGG 
CAGACGACAT TCGAGAAGCG AATGGGCTTG TTTGCGTGGG AAACGGAGGT CACCCATCCA 
CTGGCCGCCT ACGAAGGCTG CAAGCTGGTC AGCAAGCCCA TACTGATCGC GCCCCATTTG 
ATTTGGCTGC AGCTGCTCTC CGAAATGGTG GACACAGCCA AGTATTGCAA CAGGGACAAG 
GTGGAGATGT TCTGCCTCCT GTTGCACCGA TGTCTTCCCG TTCTCAAGAG TAGCAAACAG 
AACCGTCAGG TGTCCACCGT TGGCTGCCGC TTCAAGCTGC TGCAGTGCGG TCTGTCGCTG 
CTGCAGGGCA ACACCATTCC GAAGTCGCTC TCCAGAAATA TCCTCCGCGA GCGGATATAC 
TCCAACGCAC TGGACTACTT TTGCGGACCA CCAACGTGCC CCAATCAGAG CAGGGAGCAG 
CTACTGGAGG ACATAATGAT ATTGCTGAAG TTCTGGCAGA CGATGCGCAG TGAAAAGAAG 
CATCTGGTGA CGTCGGAAGT GGGTGACTAT GACTTGACAA ATGCCTCCGT TAGCTCCACG 
CAAATGCTGG CCGTGAGAAA TAATCCGGAA ACGGCATCCC TGATCAGCGG CGGTGGGCTA 
GTCAACGATT ACACGCGCTC CATGAGTGCC AGTGGCAATG CGGTGGGCAT GGGCATGGGT 
GTAGCGGGCG GCGGAAGCAG CAGTGGCTGG TACAACACCA TTCCGCACTC CACATCCACA 
CTGTCGAAGC GATCGAATCG CTCCAAGCGG CTGCAGTATC AGAAGGATTC CTACGACAAG 
GACTACATGA AGAAGCGCAA TCTGATCCTT GAGCTGCTGG CCGTCGAGCT CGAGTTCCTC 
ATTACCTGGT ACAATCCCAA TAGTCTGCCG GATCTTATAG TGCCGGGCGA GGAACAGATC 
ACGGAATGGC GCAACCGGCC GTACAAGTCG ACCGTTTGGC GGGACTATGC GCGTCTCGCC 
TGGTGCTATA ACCCGGCACT GGCTGTCTTC CTACCGCAGC GGATCAAGAA CGCGGAGATC 
ATTGATGAGG AGGTGTCGCG GCTGGTCTGC TCCGATCCCA TAGCGGTGTG CCACATTCCC 
GAGGCGCTCA AATACCTGTG CACCACCAAG AACCTGCTGC AGGAGAGCCC GGACCTGGTG 
TACATACTCT CGTGGTCGCC AGTGACGCCC ATCCAGGCCC TGGCCTACTT CTCACGGCAG 
TATCCATCGC ATCCGCTAAC AGCTCAGTAT GCCGTAAAGA CCCTCTCCTC GTATCCGGCG 
GAATCGGTGC TGCCGTACAT CCCGCAACTG GTCCAAGCCC TTCGGCACGA CACCATGGGC 
TATGTGGTGG AGTTCATTAA GAATATATCG CGACGGTCGC AGATAGTGGC ACACCAATTG 
ATCTGGAACA TGCAGACGAA CATGTACATG GACGAGGATC AACAGCACAA GGATCCCAAT 
CTGTACGAGG CACTCGATCA GCTATCGCAA AGTATTATCG CATCGTTCTC GGGCGCTGCC 
AAGCGGTTCT ACGAGCGGGA ATTCGACTTC TTCGGCAAGA TAACGGCCGT ATCCGGCGAA 
ATTCGCTCGT TTGCCAAGGG CATTGAGCGG AAGAACGCAT GCCTGGCGGC GCTTAGCAGG 
ATCAAGGTGC AGGGCGGCTG TTATCTGCCC TCCAATCCGG AGGCCATGGT CCTCGATATC 
GACTACAGCA GCGGCACGCC GATGCAAAGT GCGGCCAAGG CGCCGTATTT GGCACGATTC 
CGTGTGTATC GTTGCGGCAT CACGGAGCTG GAGACGCGCG CCATGGAGGT GTCCAATAAT 
CCGAACTCCC AGGAGGATGC CAAGATGACG CTGGGCGTGG AGTCGTGGCA GGCGGCCATT 
TTCAAGGTGG GCGACGATGT GCGCCAGGAC ATGCTGGCGC TCCAGGTGAT CACCATCTTC 
AAGAACATCT TCCAGCAGGT GGGACTCGAT CTGTTCCTGT TCCCCTACCG CGTTGTGGCC 
ACCGCTCCAG GCTGCGGCGT CATCGAGTGC GTGCCGAATG CCAAGTCGAG GGATCAACTC 
GGCCGACAGA CGGACAGTGG ACTCTCCGAG TATTTCCAGC ATCAGTACGG CGACGAGAGC 
TCCAAGGAGT TCCAGGCGGC GCGCGCCAAC TTTGTCAAGT CCATGGCAGC CTACTCGTTG 
ATCGGCTATC TGCTCCAGAT TAAGGATCGC CACAATGGTA ACATCATGAT CGACAAGGAT 
GGCCACATCA TACACATAGA TTTTGGCTTT ATGTTCGAGT CGTCGCCAGG TGGTAATATC 
GGCTTCGAGC CGGACATGAA GCTGACCGAC GAAATGGTCA TGATCATGGG CGGCAAGATG 
GACTCGCCGG CCTTCAAGTG GTTCTGCGAG CTGTGCGTGC AGGCCTTCCT CGCCGTTCGT 
CCGTATCAGG ATGCAATTGT CTCGCTGGTA TCCCTGATGC TGGACACTGG ACTGCCCTGC 
TTCCGCGGCC AGACGATCAA TCTGCTGAAG CAGCGCTTCG TGGCCACCAA GAACAACAAG 
GAGGCGGCCG CCCACATGCT GGCTGTCATC CGCAATTCGT ACCAGAACTT CCGCACGCGT 
ACCTACGACA TGATCCAGTA CTACCAGAAC CAGATACCCT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PI4KIIIalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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