pi4ka cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pi4ka
Entrez Gene ID	100127709
Full Name	phosphatidylinositol 4-kinase, catalytic, alpha
General protein information	Preferred Names phosphatidylinositol 4-kinase, catalytic, alpha Names phosphatidylinositol 4-kinase alpha
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012970537.2	XP_012825991.1	phosphatidylinositol 4-kinase alpha isoform X1
XM_012970578.2	XP_012826032.1	phosphatidylinositol 4-kinase alpha isoform X2
XM_031905856.1	XP_031761716.1	phosphatidylinositol 4-kinase alpha isoform X1
NM_001251875.2	NP_001238804.1	phosphatidylinositol 4-kinase alpha
XM_031905885.1	XP_031761745.1	phosphatidylinositol 4-kinase alpha isoform X2

Pathways from BioSystems

Homologies

	PI4KA	XP_001086542.2
Gallus gallus (chicken)	PI4KA	XP_004945537.1
Bos taurus (cattle)	PI4KA	NP_777002.1
Drosophila melanogaster (fruit fly)	PI4KIIIalpha	NP_570014.3
Caenorhabditis elegans (roundworm)	CELE_Y75B8A.24	NP_499596.2
Oryza sativa (rice)	Os03g0711200	NP_001051054.1
Xenopus tropicalis (tropical clawed frog)	pi4ka	NP_001238804.1
Homo sapiens (human)	PI4KA	NP_477352.3
Pan troglodytes (chimpanzee)	PI4KA	XP_525639.3
Mus musculus (house mouse)	Pi4ka	NP_001001983.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010801	XP_318507.4
Canis lupus familiaris (dog)	PI4KA	XP_543569.5
Rattus norvegicus (Norway rat)	Pi4ka	NP_071637.1
Danio rerio (zebrafish)	pi4kaa	NP_001030144.1
Arabidopsis thaliana (thale cress)	ATPI4K ALPHA	NP_175359.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pi4ka gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pi4ka cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa31026	XM_012970537.2 Latest version!	Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa31027	XM_012970578.2 Latest version!	Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa47456	XM_031905856.1 Latest version!	Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa47457	XM_031905885.1 Latest version!	Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa20413	NM_001251875.2 Latest version!	Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pi4ka ( XM_012970537.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825991.1 Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31026
Clone ID Related Accession (Same CDS sequence)	XM_012970537.2
Accession Version	XM_012970537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphatidylinositol 4-kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCTA TCGTCACTTT TGTATGTGAA TCACATGACA ACTGTAGCGG TGTGACGTCG 
GCTGACGGCT CCGGGAGTAA CATGGCGGCC AAGGGGGCTT GCAGCGGGTC GCGGGGGTTT 
TATTTTAACA CTGTGCTGTC GCTGGCCCGT TCCCTGGCAG TGCAGAGGCC TGCTGCATTT 
GAGAAGGTGC AGAAGCTGCT GTGCATGTGC CCTGTCGATT ATCATGGGGT TTTTCAGCTG 
GATGAAAGGC GAAGAGATGC ACTTATTGCT TTAGGAATAT TTCTGGTGGA GTCAGATCTT 
CAGCACAAAG AGACTGTAGT TCCATACCTT TTGAGACTCT TGAGGGGACT TCCAAAGGTG 
CAGTGGATAG AGGAAAGCAA TTCTCGTAAG GGAAAAGGGA CACTTCCAGT GGCAGAAACC 
TTCAGCTTCT GTTTAGTTAC ACTACTGTCG GATGTTGCGC ACAGGGATCT GTCCTTCAGA 
GAGCAGATCT TAGAAGCTGT TTTGGATGTG ATGCAGTGTC TGCTGGGAAT GTGCCAGTCA 
CCCCAAATTC AGGATAAAGA ATACTTGTGT AAATATGCAG TTCCATGCTT GATTGGAATC 
TCACGGGCCT TTGGACGATA CAGCAACACT GAAGAGTCTC TTTTATCCAA GCTGTTCCCC 
AAAGTTTCCC CACAGTCACT AAAGGCGCCA GAGGAATTAG AAGGAATACG TAGACGTTCC 
TTTAATGACT TCCGCTCCAT CCTGCCAAGC TCACTGCTTA CAGTGTGCCA GGGGGATTCT 
CTAAAGCGGA AAGCCAGCAG TGTTTCAAGT GGCTCACAGG TTAGCCCTGA TCGCATTGTT 
CCTCCTCCAG GCTCACCTGC CGGCTCTACT ATTCCTTACT TTGAAGGGTC ATATTTGCCA 
GATGGCAGTG CTGTGGATCC TGACTATTAC CTTACCACCA TCAGCTCTAG TTTCTCAGTC 
TCACCTCTCT TCACTGGTGT CAACAACAAG GAGTTTGATA TGCCCCAAGA TACTTTGCGT 
CAGCTACTCC ATATGGTAAA ACAGATTGTG GCAGACCCAT TCCTGAGTTC TTTGGATATG 
CTCATCTCTG AAGTTACAGA AGCCAATCCT AGTGTGGACC TATATTATAA AACATTCAGT 
GATCCCCTCT ACGTGGCAGT GTTTAAGATG CTCAGAGATA CACTTTATAA TGTTAAAGAT 
CCCCATACTT CATTTGTGAA GGAGATTCAT GACTTTGTGC TTGAACAGTT TAACAGCAGC 
CAGGGTGAGC TGCAGAAAAT TCTACATGAT ATGGATCGAC TGAATAATGA ACTCAGCCCC 
TTGAAATTAC GTTGTCAGGC AAATGCAGCA TGTGTGGACC TTATGGTGTG GGCTGTCAAA 
GATGAACAAG GTGCAGAAAA TCTTTGCATC AAACTATCTG AAAAGCTGCA GTCCAAGACA 
TCTAGCAAAG TGATTATAGC CCATATGCCA CTTCTAATAT GCTGTTTACA GGGGCTAGGG 
CGTCTTTGTG AGCGGTTTCC TGTAGTTGTC CATTCTGTGA CGCCTTCGCT TCGAGATTTT 
CTTGTGATCC CATCTCCGGT GCTGGTGAAG CTTTATAAAT ACCATAGCCA ATATCAGACA 
GGTGCTAATG ATATAAAGAT CAGTGTAACC AATGAGCATT CCGAATCCAC TATGAACATT 
ATGTCTGGCA AGAAAAGCCA GGCATCTATG TATGAACAGC TGCGGGATAT TGCCATAGAT 
AACATCTGCA GGTGTTTAAA AGCTGGGTTA ACCATAGATC CTGTCATTGT TGAAGCTTTT 
CTTGCCAGTT TATCAAATCG CTTGTATATA TCTCAGGAGA ATGAAAAGGA TGCTCATTTG 
ATACCTGATC ATACAATCCG TGCGTTGGGG CACATTGCTG TTGCTCTAAG GGACACCCCC 
AAAGTGATGC AACCCATCCT GCAGATCCTT CAGCAGAAGT TCTGCCAGCC CCCTTCACAG 
CTGGATGTGC TCATTATAGA CCAACTAGGG TGCATGGTGA TCACTGGCAA TCAATATATC 
TATCAAGAAG TGTGGAACCT TTTCCAACAG ATTAGTGTGA AGGCCAGTTC AGTGGTATAC 
TCTGCCACCA AGGACTATAA AGACCATGGC TACAGACACT GCTCCTTGGC TGTGATTAAC 
GCCCTGGCAA ATATTGCAGC CAACATCCAG GGAGAGCACC AGATAGATGA TTTGCTGATG 
AATTTGTTGG AGCTTTTTGT GCAGCTAGGA CTAGAAGGAA AGAGAGCCAG TGAGAGGGCT 
AGCGAAAAGG GGCCTGCATT GAAGGCCTCC AGCAGTGCAG GAAATCTGGG AGTGCTGATA 
CCCGTAATTG CTGTGCTCAC CCGAAGGCTT CCACCAATAA AAGAGGCCAA GCCACGACTT 
CAGAAACTCT TCAGAGATTT TTGGTTGTAC TCTGTGCTGA TGGGTTTTGC AGTGGAAGGA 
TCAGGTCTCT GGCCTGAGGA ATGGTATGAA GGTGTCTGTG AAATTGCCAC TAAATCTCCT 
TTGCTGACGT TCCCCAGCAA AGAGCCTCTG CGCTCTGTAC TGCAGTACAA CTCTGCCATG 
AAAAATGACA CTGTAACGCC AGCAGAACTT AATGAGCTGA GAAGCACAAT TATCAACCTT 
CTTGATCCTG CTCCGGAAGT GTCTGCACTC ATCAACAAAC TGGACTTTGC CATGTCTACC 
TACCTGCTCT CTGTGTACCG CCTGGAGCAC ATGAGGGTTC TGCATGCCAC TGATAAGGAT 
CGATTTCAAG TTATGTTTTT TTACTTCGAG GATAAAGCTA TCCAAAAGGA TAAATCAGGT 
ATGATGCAGT GTGTTATTGC AGTAGCAGAC AAAGTGTTTG AAGCTTTTCT GAACGTGATG 
GCTGATAAGC CTAAAACGAA AGAGAACGAG GAAGAATTGG AGCGCCATGC ACAATTCCTG 
CTGGTAAACT TTAATCACAT CCACAAGCGC ATTCGACGGG TCGCTGACAA ATACTTGTCA 
GGGCTAGTGG ACAAATTTCC CCATTTGCTG TGGAGTGGTA CTGTGCTGAA AACCATGCTG 
GACATACTGC AGACTCTTTC CTTGTCTCTT AGTGCAGACA TTCACAAAGA CCAGCCTTAT 
TATGACATTC CAGATGCACC TTATAGAATA ACCGTTCCCG ATACCTATGA GGCCAGAGAG 
AGCATTGTCA AAGACTTTGC AGCTCGATGT GGCATGATTC TTCAGGAAGC AATGAAATGG 
GCTCCTTCAG TGACCAAGTC CCACCTGCAG GAATATCTAA ACAAGCACCA GAACTGGATG 
TCTGGCCTTT CCCAGCACAC TGGTCTTGCC ATGGCTACTG AAAGCATACT GCATTTTGCT 
GGGTACAACA GGCAGAATAC TACCTTGGGG TCTCCAGCTT CCTCTGCCCA GGTGACACAA 
CTAACAGAAA GGCCAGCCTG TGTCAAGAAG GATTATTCAA ACTTCATGGC ATCTCTCAAC 
CTGCGCAACC GTTATGCTGG AGAGGTTGCA GGCATGATTG AGTTTTCTGA TGCCACTGGC 
CGAACTTCTG ACTTAAATAG GCTCATGCTG AAGCACCTGG GCGATGCCTT GGACTGCGGC 
CAACCAGAGT CTTATACACA GGTCATGTTC AAACTTACTG CGCTGCTCAT TAGCAGCAAA 
GAGTGTGATG CTCAGCTTCT GCACCACCTG TGCTGGGCAC CCCTGCGGAT GTTCACTGAT 
CATGGTATGG AGACCGCTAT TGCCTGCTGG GACTGGTTGC TTGCAGCTAA AAATGGCATT 
GAAGTACCGT TTATGCGTGA AATGGCTGGT GCTTGGCAAA TGACTGTAGA GCAAAAGATT 
GGACTTTTCT CAGCAGAAAA GAAGGAGGCA GATCCTCTTG CTGCATCTGA ACAGAGCCAA 
CCACGTCCAT GTGCACCACA AGTGAACCCA CATTATATTT GGATTGATTT TCTCATGCAA 
AGGTTTGAGA TTGCCAAATA CTGCAGCTCT GATCAGGTGG AAATCTTCTC CAGCCTTCTG 
CAGCGCTCCC TGTCCCTAAA CATTGGTGGT TGTAAAGGAA GTCTTAACCG ACATGTGGCA 
GCTATTGGCC CTCGATTTAA GTTGCTGACT CTCGGGCTGT CCCTGTTACA TGCAGATGTG 
GTCCCAAATG CGACAATCCG GAATGTCCTT AGAGAAAAGA TCTACTCTAC AGCATTTGAT 
TATTTTAGTT GCCCTCCTAA GTTCCCGACT CAGACGCAGA AGCTGCTAAG GGAGGATATC 
AGCATCATGA TCAAGTTCTG GACAGCCATG TTTTCTGATA AAAAATATCT CACTGCCAAT 
CAGCTAGTAC CCCCAGATAA TCAAGACCCT TCTGTAAACA GCTTGTCTGT GATGTCAGAT 
GTGAACCGCA GCAATTTGGA TGTGACTGTT GGCTCCCGAC AGCAAACAGC TCAGGGCTGG 
ATCAATACAT ACCCTCTGTC CAGTGGAATG TCCACCATCT CTAAAAAATC TGGTTTAGCA 
AAGAAAACAA ACCGTGGGAC TCAGCTGCAC AAGTACTATA TGAAGAGGCG GACTCTGTTG 
CTGTCACTTT TGGCCACTGA GATTGAACGC CTGACCACAT GGTACAACCC CTTGTCCTCC 
CCTGAGTTGG AACTGGATCA GACAGGTGAA AACAGTGTGG CTAACTGGAG ATCTAAGTAC 
ATTAGTTTGA GTGAGAAGCA GTGGAAGGAC AATGTGAACT TAGCATGGAG CATCTCCCCT 
CACTTGGCTT TGCAATTACC CACCAGGTTC AAGAACACTG AGGCTATTGG GGGAGAAGTG 
ACCCGCCTGG TTAGATTGGA CCCAGGAGCA GTGAGTGACA CTCCAGAAGC AATAAAGTAC 
TTGGTAACAT GGCACACAAT AGATGCTGAT GCTCCTGAAC TTAGTCACAT ACTCTGCTGG 
ACACCGACTG ACCCACCTAC AGGACTCTCC TACTTCTCTA GCATGTACCC ACCACATCCA 
CTTACTGCGC AATATGGCGT GAAAGTGCTG CGATCATTCC CACCGGATGC AATCTTGTTC 
TACATTCCGC AGATAGTTCA AGCCTTGAGA TATGATAAGA TGGGTTACGT TCGAGAATAC 
ATTCTATGGG CAGCTGCCAA ATCACAGCTT TTGGCTCATC AGTTTATTTG GAACATGAAG 
ACAAATATTT TCCTTGATGA GGAAGGGCAC CAAAAAGACC CTGATATTGG AGAACTATTG 
GAGCAGCTGG TTGAAGAAAT TACAGGATCT CTGTCTGGGC CAGCCAAGGA TTTCTACCAG 
CGAGAATTTG ACTTTTTCAA TAAGATCACA AATGTCTCTG CCATCATTAA GCCATTTCCA 
AAAGGAGAAG AACGGAAAAA GGCCTGTCTG AACTCTCTTT CAGAAGTAAA AGTGCAGCCA 
GGTTGTTACC TGCCCAGTAA TCCAGAAGCC ATTGTGTTGG ACATTGATTA CACATCTGGG 
ACCCCTTTGC AGAGTGCTGC GAAGGCTCCT TATTTGGCCA AATTTAAGGT GAAGCGATGT 
GGAGTAAGTG AACTGGAGAA GGAAGGTCTC AGCTGCCGTT CAGATTCTAT TGATGAGAGT 
GAGGAGGAAG GTGGAGATGG GAAGGTTTGC TGGCAAGCGG CTATCTTTAA GGTCGGAGAT 
GACTGCAGAC AGGTGAGCAG AATATCTTTT CTGCGTTACT GTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825991.1
CDS	8..5695
Translation	MLPIVTFVCESHDNCSGVTSADGSGSNMAAKGACSGSRGFYFNTVLSLARSLAVQRPAAFEKVQKLLCMCPVDYHGVFQLDERRRDALIALGIFLVESDLQHKETVVPYLLRLLRGLPKVQWIEESNSRKGKGTLPVAETFSFCLVTLLSDVAHRDLSFREQILEAVLDVMQCLLGMCQSPQIQDKEYLCKYAVPCLIGISRAFGRYSNTEESLLSKLFPKVSPQSLKAPEELEGIRRRSFNDFRSILPSSLLTVCQGDSLKRKASSVSSGSQVSPDRIVPPPGSPAGSTIPYFEGSYLPDGSAVDPDYYLTTISSSFSVSPLFTGVNNKEFDMPQDTLRQLLHMVKQIVADPFLSSLDMLISEVTEANPSVDLYYKTFSDPLYVAVFKMLRDTLYNVKDPHTSFVKEIHDFVLEQFNSSQGELQKILHDMDRLNNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHMPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYQTGANDIKISVTNEHSESTMNIMSGKKSQASMYEQLRDIAIDNICRCLKAGLTIDPVIVEAFLASLSNRLYISQENEKDAHLIPDHTIRALGHIAVALRDTPKVMQPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQGEHQIDDLLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELNELRSTIINLLDPAPEVSALINKLDFAMSTYLLSVYRLEHMRVLHATDKDRFQVMFFYFEDKAIQKDKSGMMQCVIAVADKVFEAFLNVMADKPKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPSVTKSHLQEYLNKHQNWMSGLSQHTGLAMATESILHFAGYNRQNTTLGSPASSAQVTQLTERPACVKKDYSNFMASLNLRNRYAGEVAGMIEFSDATGRTSDLNRLMLKHLGDALDCGQPESYTQVMFKLTALLISSKECDAQLLHHLCWAPLRMFTDHGMETAIACWDWLLAAKNGIEVPFMREMAGAWQMTVEQKIGLFSAEKKEADPLAASEQSQPRPCAPQVNPHYIWIDFLMQRFEIAKYCSSDQVEIFSSLLQRSLSLNIGGCKGSLNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQTQKLLREDISIMIKFWTAMFSDKKYLTANQLVPPDNQDPSVNSLSVMSDVNRSNLDVTVGSRQQTAQGWINTYPLSSGMSTISKKSGLAKKTNRGTQLHKYYMKRRTLLLSLLATEIERLTTWYNPLSSPELELDQTGENSVANWRSKYISLSEKQWKDNVNLAWSISPHLALQLPTRFKNTEAIGGEVTRLVRLDPGAVSDTPEAIKYLVTWHTIDADAPELSHILCWTPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPFPKGEERKKACLNSLSEVKVQPGCYLPSNPEAIVLDIDYTSGTPLQSAAKAPYLAKFKVKRCGVSELEKEGLSCRSDSIDESEEEGGDGKVCWQAAIFKVGDDCRQVSRISFLRYCK

Target ORF information:

RefSeq Version	XM_012970537.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970537.2

ORF Insert Sequence:

ATGTTGCCTA TCGTCACTTT TGTATGTGAA TCACATGACA ACTGTAGCGG TGTGACGTCG 
GCTGACGGCT CCGGGAGTAA CATGGCGGCC AAGGGGGCTT GCAGCGGGTC GCGGGGGTTT 
TATTTTAACA CTGTGCTGTC GCTGGCCCGT TCCCTGGCAG TGCAGAGGCC TGCTGCATTT 
GAGAAGGTGC AGAAGCTGCT GTGCATGTGC CCTGTCGATT ATCATGGGGT TTTTCAGCTG 
GATGAAAGGC GAAGAGATGC ACTTATTGCT TTAGGAATAT TTCTGGTGGA GTCAGATCTT 
CAGCACAAAG AGACTGTAGT TCCATACCTT TTGAGACTCT TGAGGGGACT TCCAAAGGTG 
CAGTGGATAG AGGAAAGCAA TTCTCGTAAG GGAAAAGGGA CACTTCCAGT GGCAGAAACC 
TTCAGCTTCT GTTTAGTTAC ACTACTGTCG GATGTTGCGC ACAGGGATCT GTCCTTCAGA 
GAGCAGATCT TAGAAGCTGT TTTGGATGTG ATGCAGTGTC TGCTGGGAAT GTGCCAGTCA 
CCCCAAATTC AGGATAAAGA ATACTTGTGT AAATATGCAG TTCCATGCTT GATTGGAATC 
TCACGGGCCT TTGGACGATA CAGCAACACT GAAGAGTCTC TTTTATCCAA GCTGTTCCCC 
AAAGTTTCCC CACAGTCACT AAAGGCGCCA GAGGAATTAG AAGGAATACG TAGACGTTCC 
TTTAATGACT TCCGCTCCAT CCTGCCAAGC TCACTGCTTA CAGTGTGCCA GGGGGATTCT 
CTAAAGCGGA AAGCCAGCAG TGTTTCAAGT GGCTCACAGG TTAGCCCTGA TCGCATTGTT 
CCTCCTCCAG GCTCACCTGC CGGCTCTACT ATTCCTTACT TTGAAGGGTC ATATTTGCCA 
GATGGCAGTG CTGTGGATCC TGACTATTAC CTTACCACCA TCAGCTCTAG TTTCTCAGTC 
TCACCTCTCT TCACTGGTGT CAACAACAAG GAGTTTGATA TGCCCCAAGA TACTTTGCGT 
CAGCTACTCC ATATGGTAAA ACAGATTGTG GCAGACCCAT TCCTGAGTTC TTTGGATATG 
CTCATCTCTG AAGTTACAGA AGCCAATCCT AGTGTGGACC TATATTATAA AACATTCAGT 
GATCCCCTCT ACGTGGCAGT GTTTAAGATG CTCAGAGATA CACTTTATAA TGTTAAAGAT 
CCCCATACTT CATTTGTGAA GGAGATTCAT GACTTTGTGC TTGAACAGTT TAACAGCAGC 
CAGGGTGAGC TGCAGAAAAT TCTACATGAT ATGGATCGAC TGAATAATGA ACTCAGCCCC 
TTGAAATTAC GTTGTCAGGC AAATGCAGCA TGTGTGGACC TTATGGTGTG GGCTGTCAAA 
GATGAACAAG GTGCAGAAAA TCTTTGCATC AAACTATCTG AAAAGCTGCA GTCCAAGACA 
TCTAGCAAAG TGATTATAGC CCATATGCCA CTTCTAATAT GCTGTTTACA GGGGCTAGGG 
CGTCTTTGTG AGCGGTTTCC TGTAGTTGTC CATTCTGTGA CGCCTTCGCT TCGAGATTTT 
CTTGTGATCC CATCTCCGGT GCTGGTGAAG CTTTATAAAT ACCATAGCCA ATATCAGACA 
GGTGCTAATG ATATAAAGAT CAGTGTAACC AATGAGCATT CCGAATCCAC TATGAACATT 
ATGTCTGGCA AGAAAAGCCA GGCATCTATG TATGAACAGC TGCGGGATAT TGCCATAGAT 
AACATCTGCA GGTGTTTAAA AGCTGGGTTA ACCATAGATC CTGTCATTGT TGAAGCTTTT 
CTTGCCAGTT TATCAAATCG CTTGTATATA TCTCAGGAGA ATGAAAAGGA TGCTCATTTG 
ATACCTGATC ATACAATCCG TGCGTTGGGG CACATTGCTG TTGCTCTAAG GGACACCCCC 
AAAGTGATGC AACCCATCCT GCAGATCCTT CAGCAGAAGT TCTGCCAGCC CCCTTCACAG 
CTGGATGTGC TCATTATAGA CCAACTAGGG TGCATGGTGA TCACTGGCAA TCAATATATC 
TATCAAGAAG TGTGGAACCT TTTCCAACAG ATTAGTGTGA AGGCCAGTTC AGTGGTATAC 
TCTGCCACCA AGGACTATAA AGACCATGGC TACAGACACT GCTCCTTGGC TGTGATTAAC 
GCCCTGGCAA ATATTGCAGC CAACATCCAG GGAGAGCACC AGATAGATGA TTTGCTGATG 
AATTTGTTGG AGCTTTTTGT GCAGCTAGGA CTAGAAGGAA AGAGAGCCAG TGAGAGGGCT 
AGCGAAAAGG GGCCTGCATT GAAGGCCTCC AGCAGTGCAG GAAATCTGGG AGTGCTGATA 
CCCGTAATTG CTGTGCTCAC CCGAAGGCTT CCACCAATAA AAGAGGCCAA GCCACGACTT 
CAGAAACTCT TCAGAGATTT TTGGTTGTAC TCTGTGCTGA TGGGTTTTGC AGTGGAAGGA 
TCAGGTCTCT GGCCTGAGGA ATGGTATGAA GGTGTCTGTG AAATTGCCAC TAAATCTCCT 
TTGCTGACGT TCCCCAGCAA AGAGCCTCTG CGCTCTGTAC TGCAGTACAA CTCTGCCATG 
AAAAATGACA CTGTAACGCC AGCAGAACTT AATGAGCTGA GAAGCACAAT TATCAACCTT 
CTTGATCCTG CTCCGGAAGT GTCTGCACTC ATCAACAAAC TGGACTTTGC CATGTCTACC 
TACCTGCTCT CTGTGTACCG CCTGGAGCAC ATGAGGGTTC TGCATGCCAC TGATAAGGAT 
CGATTTCAAG TTATGTTTTT TTACTTCGAG GATAAAGCTA TCCAAAAGGA TAAATCAGGT 
ATGATGCAGT GTGTTATTGC AGTAGCAGAC AAAGTGTTTG AAGCTTTTCT GAACGTGATG 
GCTGATAAGC CTAAAACGAA AGAGAACGAG GAAGAATTGG AGCGCCATGC ACAATTCCTG 
CTGGTAAACT TTAATCACAT CCACAAGCGC ATTCGACGGG TCGCTGACAA ATACTTGTCA 
GGGCTAGTGG ACAAATTTCC CCATTTGCTG TGGAGTGGTA CTGTGCTGAA AACCATGCTG 
GACATACTGC AGACTCTTTC CTTGTCTCTT AGTGCAGACA TTCACAAAGA CCAGCCTTAT 
TATGACATTC CAGATGCACC TTATAGAATA ACCGTTCCCG ATACCTATGA GGCCAGAGAG 
AGCATTGTCA AAGACTTTGC AGCTCGATGT GGCATGATTC TTCAGGAAGC AATGAAATGG 
GCTCCTTCAG TGACCAAGTC CCACCTGCAG GAATATCTAA ACAAGCACCA GAACTGGATG 
TCTGGCCTTT CCCAGCACAC TGGTCTTGCC ATGGCTACTG AAAGCATACT GCATTTTGCT 
GGGTACAACA GGCAGAATAC TACCTTGGGG TCTCCAGCTT CCTCTGCCCA GGTGACACAA 
CTAACAGAAA GGCCAGCCTG TGTCAAGAAG GATTATTCAA ACTTCATGGC ATCTCTCAAC 
CTGCGCAACC GTTATGCTGG AGAGGTTGCA GGCATGATTG AGTTTTCTGA TGCCACTGGC 
CGAACTTCTG ACTTAAATAG GCTCATGCTG AAGCACCTGG GCGATGCCTT GGACTGCGGC 
CAACCAGAGT CTTATACACA GGTCATGTTC AAACTTACTG CGCTGCTCAT TAGCAGCAAA 
GAGTGTGATG CTCAGCTTCT GCACCACCTG TGCTGGGCAC CCCTGCGGAT GTTCACTGAT 
CATGGTATGG AGACCGCTAT TGCCTGCTGG GACTGGTTGC TTGCAGCTAA AAATGGCATT 
GAAGTACCGT TTATGCGTGA AATGGCTGGT GCTTGGCAAA TGACTGTAGA GCAAAAGATT 
GGACTTTTCT CAGCAGAAAA GAAGGAGGCA GATCCTCTTG CTGCATCTGA ACAGAGCCAA 
CCACGTCCAT GTGCACCACA AGTGAACCCA CATTATATTT GGATTGATTT TCTCATGCAA 
AGGTTTGAGA TTGCCAAATA CTGCAGCTCT GATCAGGTGG AAATCTTCTC CAGCCTTCTG 
CAGCGCTCCC TGTCCCTAAA CATTGGTGGT TGTAAAGGAA GTCTTAACCG ACATGTGGCA 
GCTATTGGCC CTCGATTTAA GTTGCTGACT CTCGGGCTGT CCCTGTTACA TGCAGATGTG 
GTCCCAAATG CGACAATCCG GAATGTCCTT AGAGAAAAGA TCTACTCTAC AGCATTTGAT 
TATTTTAGTT GCCCTCCTAA GTTCCCGACT CAGACGCAGA AGCTGCTAAG GGAGGATATC 
AGCATCATGA TCAAGTTCTG GACAGCCATG TTTTCTGATA AAAAATATCT CACTGCCAAT 
CAGCTAGTAC CCCCAGATAA TCAAGACCCT TCTGTAAACA GCTTGTCTGT GATGTCAGAT 
GTGAACCGCA GCAATTTGGA TGTGACTGTT GGCTCCCGAC AGCAAACAGC TCAGGGCTGG 
ATCAATACAT ACCCTCTGTC CAGTGGAATG TCCACCATCT CTAAAAAATC TGGTTTAGCA 
AAGAAAACAA ACCGTGGGAC TCAGCTGCAC AAGTACTATA TGAAGAGGCG GACTCTGTTG 
CTGTCACTTT TGGCCACTGA GATTGAACGC CTGACCACAT GGTACAACCC CTTGTCCTCC 
CCTGAGTTGG AACTGGATCA GACAGGTGAA AACAGTGTGG CTAACTGGAG ATCTAAGTAC 
ATTAGTTTGA GTGAGAAGCA GTGGAAGGAC AATGTGAACT TAGCATGGAG CATCTCCCCT 
CACTTGGCTT TGCAATTACC CACCAGGTTC AAGAACACTG AGGCTATTGG GGGAGAAGTG 
ACCCGCCTGG TTAGATTGGA CCCAGGAGCA GTGAGTGACA CTCCAGAAGC AATAAAGTAC 
TTGGTAACAT GGCACACAAT AGATGCTGAT GCTCCTGAAC TTAGTCACAT ACTCTGCTGG 
ACACCGACTG ACCCACCTAC AGGACTCTCC TACTTCTCTA GCATGTACCC ACCACATCCA 
CTTACTGCGC AATATGGCGT GAAAGTGCTG CGATCATTCC CACCGGATGC AATCTTGTTC 
TACATTCCGC AGATAGTTCA AGCCTTGAGA TATGATAAGA TGGGTTACGT TCGAGAATAC 
ATTCTATGGG CAGCTGCCAA ATCACAGCTT TTGGCTCATC AGTTTATTTG GAACATGAAG 
ACAAATATTT TCCTTGATGA GGAAGGGCAC CAAAAAGACC CTGATATTGG AGAACTATTG 
GAGCAGCTGG TTGAAGAAAT TACAGGATCT CTGTCTGGGC CAGCCAAGGA TTTCTACCAG 
CGAGAATTTG ACTTTTTCAA TAAGATCACA AATGTCTCTG CCATCATTAA GCCATTTCCA 
AAAGGAGAAG AACGGAAAAA GGCCTGTCTG AACTCTCTTT CAGAAGTAAA AGTGCAGCCA 
GGTTGTTACC TGCCCAGTAA TCCAGAAGCC ATTGTGTTGG ACATTGATTA CACATCTGGG 
ACCCCTTTGC AGAGTGCTGC GAAGGCTCCT TATTTGGCCA AATTTAAGGT GAAGCGATGT 
GGAGTAAGTG AACTGGAGAA GGAAGGTCTC AGCTGCCGTT CAGATTCTAT TGATGAGAGT 
GAGGAGGAAG GTGGAGATGG GAAGGTTTGC TGGCAAGCGG CTATCTTTAA GGTCGGAGAT 
GACTGCAGAC AGGTGAGCAG AATATCTTTT CTGCGTTACT GTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pi4ka ( XM_012970578.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826032.1 Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X2, mRNA.

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CloneID	OXa31027
Clone ID Related Accession (Same CDS sequence)	XM_012970578.2
Accession Version	XM_012970578.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphatidylinositol 4-kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCTA TCGTCACTTT TGTATGTGAA TCACATGACA ACTGTAGCGG TGTGACGTCG 
GCTGACGGCT CCGGGAGTAA CATGGCGGCC AAGGGGGCTT GCAGCGGGTC GCGGGGGTTT 
TATTTTAACA CTGTGCTGTC GCTGGCCCGT TCCCTGGCAG TGCAGAGGCC TGCTGCATTT 
GAGAAGGTGC AGAAGCTGCT GTGCATGTGC CCTGTCGATT ATCATGGGGT TTTTCAGCTG 
GATGAAAGGC GAAGAGATGC ACTTATTGCT TTAGGAATAT TTCTGGTGGA GTCAGATCTT 
CAGCACAAAG AGACTGTAGT TCCATACCTT TTGAGACTCT TGAGGGGACT TCCAAAGGTG 
CAGTGGATAG AGGAAAGCAA TTCTCGTAAG GGAAAAGGGA CACTTCCAGT GGCAGAAACC 
TTCAGCTTCT GTTTAGTTAC ACTACTGTCG GATGTTGCGC ACAGGGATCT GTCCTTCAGA 
GAGCAGATCT TAGAAGCTGT TTTGGATGTG ATGCAGTGTC TGCTGGGAAT GTGCCAGTCA 
CCCCAAATTC AGGATAAAGA ATACTTGTGT AAATATGCAG TTCCATGCTT GATTGGAATC 
TCACGGGCCT TTGGACGATA CAGCAACACT GAAGAGTCTC TTTTATCCAA GCTGTTCCCC 
AAAGTTTCCC CACAGTCACT AAAGGCGCCA GAGGAATTAG AAGGAATACG TAGACGTTCC 
TTTAATGACT TCCGCTCCAT CCTGCCAAGC TCACTGCTTA CAGTGTGCCA GGGGGATTCT 
CTAAAGCGGA AAGCCAGCAG TGTTTCAAGT GGCTCACAGG TTAGCCCTGA TCGCATTGTT 
CCTCCTCCAG GCTCACCTGC CGGCTCTACT ATTCCTTACT TTGAAGGGTC ATATTTGCCA 
GATGGCAGTG CTGTGGATCC TGACTATTAC CTTACCACCA TCAGCTCTAG TTTCTCAGTC 
TCACCTCTCT TCACTGGTGT CAACAACAAG GAGTTTGATA TGCCCCAAGA TACTTTGCGT 
CAGCTACTCC ATATGGTAAA ACAGATTGTG GCAGACCCAT TCCTGAGTTC TTTGGATATG 
CTCATCTCTG AAGTTACAGA AGCCAATCCT AGTGTGGACC TATATTATAA AACATTCAGT 
GATCCCCTCT ACGTGGCAGT GTTTAAGATG CTCAGAGATA CACTTTATAA TGTTAAAGAT 
CCCCATACTT CATTTGTGAA GGAGATTCAT GACTTTGTGC TTGAACAGTT TAACAGCAGC 
CAGGGTGAGC TGCAGAAAAT TCTACATGAT ATGGATCGAC TGAATAATGA ACTCAGCCCC 
TTGAAATTAC GTTGTCAGGC AAATGCAGCA TGTGTGGACC TTATGGTGTG GGCTGTCAAA 
GATGAACAAG GTGCAGAAAA TCTTTGCATC AAACTATCTG AAAAGCTGCA GTCCAAGACA 
TCTAGCAAAG TGATTATAGC CCATATGCCA CTTCTAATAT GCTGTTTACA GGGGCTAGGG 
CGTCTTTGTG AGCGGTTTCC TGTAGTTGTC CATTCTGTGA CGCCTTCGCT TCGAGATTTT 
CTTGTGATCC CATCTCCGGT GCTGGTGAAG CTTTATAAAT ACCATAGCCA ATATCAGACA 
GGTGCTAATG ATATAAAGAT CAGTGTAACC AATGAGCATT CCGAATCCAC TATGAACATT 
ATGTCTGGCA AGAAAAGCCA GGCATCTATG TATGAACAGC TGCGGGATAT TGCCATAGAT 
AACATCTGCA GGTGTTTAAA AGCTGGGTTA ACCATAGATC CTGTCATTGT TGAAGCTTTT 
CTTGCCAGTT TATCAAATCG CTTGTATATA TCTCAGGAGA ATGAAAAGGA TGCTCATTTG 
ATACCTGATC ATACAATCCG TGCGTTGGGG CACATTGCTG TTGCTCTAAG GGACACCCCC 
AAAGTGATGC AACCCATCCT GCAGATCCTT CAGCAGAAGT TCTGCCAGCC CCCTTCACAG 
CTGGATGTGC TCATTATAGA CCAACTAGGG TGCATGGTGA TCACTGGCAA TCAATATATC 
TATCAAGAAG TGTGGAACCT TTTCCAACAG ATTAGTGTGA AGGCCAGTTC AGTGGTATAC 
TCTGCCACCA AGGACTATAA AGACCATGGC TACAGACACT GCTCCTTGGC TGTGATTAAC 
GCCCTGGCAA ATATTGCAGC CAACATCCAG GGAGAGCACC AGATAGATGA TTTGCTGATG 
AATTTGTTGG AGCTTTTTGT GCAGCTAGGA CTAGAAGGAA AGAGAGCCAG TGAGAGGGCT 
AGCGAAAAGG GGCCTGCATT GAAGGCCTCC AGCAGTGCAG GAAATCTGGG AGTGCTGATA 
CCCGTAATTG CTGTGCTCAC CCGAAGGCTT CCACCAATAA AAGAGGCCAA GCCACGACTT 
CAGAAACTCT TCAGAGATTT TTGGTTGTAC TCTGTGCTGA TGGGTTTTGC AGTGGAAGGA 
TCAGGTCTCT GGCCTGAGGA ATGGTATGAA GGTGTCTGTG AAATTGCCAC TAAATCTCCT 
TTGCTGACGT TCCCCAGCAA AGAGCCTCTG CGCTCTGTAC TGCAGTACAA CTCTGCCATG 
AAAAATGACA CTGTAACGCC AGCAGAACTT AATGAGCTGA GAAGCACAAT TATCAACCTT 
CTTGATCCTG CTCCGGAAGT GTCTGCACTC ATCAACAAAC TGGACTTTGC CATGTCTACC 
TACCTGCTCT CTGTGTACCG CCTGGAGCAC ATGAGGGTTC TGCATGCCAC TGATAAGGAT 
CGATTTCAAG TTATGTTTTT TTACTTCGAG GATAAAGCTA TCCAAAAGGA TAAATCAGGT 
ATGATGCAGT GTGTTATTGC AGTAGCAGAC AAAGTGTTTG AAGCTTTTCT GAACGTGATG 
GCTGATAAGC CTAAAACGAA AGAGAACGAG GAAGAATTGG AGCGCCATGC ACAATTCCTG 
CTGGTAAACT TTAATCACAT CCACAAGCGC ATTCGACGGG TCGCTGACAA ATACTTGTCA 
GGGCTAGTGG ACAAATTTCC CCATTTGCTG TGGAGTGGTA CTGTGCTGAA AACCATGCTG 
GACATACTGC AGACTCTTTC CTTGTCTCTT AGTGCAGACA TTCACAAAGA CCAGCCTTAT 
TATGACATTC CAGATGCACC TTATAGAATA ACCGTTCCCG ATACCTATGA GGCCAGAGAG 
AGCATTGTCA AAGACTTTGC AGCTCGATGT GGCATGATTC TTCAGGAAGC AATGAAATGG 
GCTCCTTCAG TGACCAAGTC CCACCTGCAG GAATATCTAA ACAAGCACCA GAACTGGATG 
TCTGGCCTTT CCCAGCACAC TGGTCTTGCC ATGGCTACTG AAAGCATACT GCATTTTGCT 
GGGTACAACA GGCAGAATAC TACCTTGGGG GTGACACAAC TAACAGAAAG GCCAGCCTGT 
GTCAAGAAGG ATTATTCAAA CTTCATGGCA TCTCTCAACC TGCGCAACCG TTATGCTGGA 
GAGGTTGCAG GCATGATTGA GTTTTCTGAT GCCACTGGCC GAACTTCTGA CTTAAATAGG 
CTCATGCTGA AGCACCTGGG CGATGCCTTG GACTGCGGCC AACCAGAGTC TTATACACAG 
GTCATGTTCA AACTTACTGC GCTGCTCATT AGCAGCAAAG AGTGTGATGC TCAGCTTCTG 
CACCACCTGT GCTGGGCACC CCTGCGGATG TTCACTGATC ATGGTATGGA GACCGCTATT 
GCCTGCTGGG ACTGGTTGCT TGCAGCTAAA AATGGCATTG AAGTACCGTT TATGCGTGAA 
ATGGCTGGTG CTTGGCAAAT GACTGTAGAG CAAAAGATTG GACTTTTCTC AGCAGAAAAG 
AAGGAGGCAG ATCCTCTTGC TGCATCTGAA CAGAGCCAAC CACGTCCATG TGCACCACAA 
GTGAACCCAC ATTATATTTG GATTGATTTT CTCATGCAAA GGTTTGAGAT TGCCAAATAC 
TGCAGCTCTG ATCAGGTGGA AATCTTCTCC AGCCTTCTGC AGCGCTCCCT GTCCCTAAAC 
ATTGGTGGTT GTAAAGGAAG TCTTAACCGA CATGTGGCAG CTATTGGCCC TCGATTTAAG 
TTGCTGACTC TCGGGCTGTC CCTGTTACAT GCAGATGTGG TCCCAAATGC GACAATCCGG 
AATGTCCTTA GAGAAAAGAT CTACTCTACA GCATTTGATT ATTTTAGTTG CCCTCCTAAG 
TTCCCGACTC AGACGCAGAA GCTGCTAAGG GAGGATATCA GCATCATGAT CAAGTTCTGG 
ACAGCCATGT TTTCTGATAA AAAATATCTC ACTGCCAATC AGCTAGTACC CCCAGATAAT 
CAAGACCCTT CTGTAAACAG CTTGTCTGTG ATGTCAGATG TGAACCGCAG CAATTTGGAT 
GTGACTGTTG GCTCCCGACA GCAAACAGCT CAGGGCTGGA TCAATACATA CCCTCTGTCC 
AGTGGAATGT CCACCATCTC TAAAAAATCT GGTTTAGCAA AGAAAACAAA CCGTGGGACT 
CAGCTGCACA AGTACTATAT GAAGAGGCGG ACTCTGTTGC TGTCACTTTT GGCCACTGAG 
ATTGAACGCC TGACCACATG GTACAACCCC TTGTCCTCCC CTGAGTTGGA ACTGGATCAG 
ACAGGTGAAA ACAGTGTGGC TAACTGGAGA TCTAAGTACA TTAGTTTGAG TGAGAAGCAG 
TGGAAGGACA ATGTGAACTT AGCATGGAGC ATCTCCCCTC ACTTGGCTTT GCAATTACCC 
ACCAGGTTCA AGAACACTGA GGCTATTGGG GGAGAAGTGA CCCGCCTGGT TAGATTGGAC 
CCAGGAGCAG TGAGTGACAC TCCAGAAGCA ATAAAGTACT TGGTAACATG GCACACAATA 
GATGCTGATG CTCCTGAACT TAGTCACATA CTCTGCTGGA CACCGACTGA CCCACCTACA 
GGACTCTCCT ACTTCTCTAG CATGTACCCA CCACATCCAC TTACTGCGCA ATATGGCGTG 
AAAGTGCTGC GATCATTCCC ACCGGATGCA ATCTTGTTCT ACATTCCGCA GATAGTTCAA 
GCCTTGAGAT ATGATAAGAT GGGTTACGTT CGAGAATACA TTCTATGGGC AGCTGCCAAA 
TCACAGCTTT TGGCTCATCA GTTTATTTGG AACATGAAGA CAAATATTTT CCTTGATGAG 
GAAGGGCACC AAAAAGACCC TGATATTGGA GAACTATTGG AGCAGCTGGT TGAAGAAATT 
ACAGGATCTC TGTCTGGGCC AGCCAAGGAT TTCTACCAGC GAGAATTTGA CTTTTTCAAT 
AAGATCACAA ATGTCTCTGC CATCATTAAG CCATTTCCAA AAGGAGAAGA ACGGAAAAAG 
GCCTGTCTGA ACTCTCTTTC AGAAGTAAAA GTGCAGCCAG GTTGTTACCT GCCCAGTAAT 
CCAGAAGCCA TTGTGTTGGA CATTGATTAC ACATCTGGGA CCCCTTTGCA GAGTGCTGCG 
AAGGCTCCTT ATTTGGCCAA ATTTAAGGTG AAGCGATGTG GAGTAAGTGA ACTGGAGAAG 
GAAGGTCTCA GCTGCCGTTC AGATTCTATT GATGAGAGTG AGGAGGAAGG TGGAGATGGG 
AAGGTTTGCT GGCAAGCGGC TATCTTTAAG GTCGGAGATG ACTGCAGACA GGTGAGCAGA 
ATATCTTTTC TGCGTTACTG TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826032.1
CDS	8..5674
Translation	MLPIVTFVCESHDNCSGVTSADGSGSNMAAKGACSGSRGFYFNTVLSLARSLAVQRPAAFEKVQKLLCMCPVDYHGVFQLDERRRDALIALGIFLVESDLQHKETVVPYLLRLLRGLPKVQWIEESNSRKGKGTLPVAETFSFCLVTLLSDVAHRDLSFREQILEAVLDVMQCLLGMCQSPQIQDKEYLCKYAVPCLIGISRAFGRYSNTEESLLSKLFPKVSPQSLKAPEELEGIRRRSFNDFRSILPSSLLTVCQGDSLKRKASSVSSGSQVSPDRIVPPPGSPAGSTIPYFEGSYLPDGSAVDPDYYLTTISSSFSVSPLFTGVNNKEFDMPQDTLRQLLHMVKQIVADPFLSSLDMLISEVTEANPSVDLYYKTFSDPLYVAVFKMLRDTLYNVKDPHTSFVKEIHDFVLEQFNSSQGELQKILHDMDRLNNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHMPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYQTGANDIKISVTNEHSESTMNIMSGKKSQASMYEQLRDIAIDNICRCLKAGLTIDPVIVEAFLASLSNRLYISQENEKDAHLIPDHTIRALGHIAVALRDTPKVMQPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQGEHQIDDLLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELNELRSTIINLLDPAPEVSALINKLDFAMSTYLLSVYRLEHMRVLHATDKDRFQVMFFYFEDKAIQKDKSGMMQCVIAVADKVFEAFLNVMADKPKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPSVTKSHLQEYLNKHQNWMSGLSQHTGLAMATESILHFAGYNRQNTTLGVTQLTERPACVKKDYSNFMASLNLRNRYAGEVAGMIEFSDATGRTSDLNRLMLKHLGDALDCGQPESYTQVMFKLTALLISSKECDAQLLHHLCWAPLRMFTDHGMETAIACWDWLLAAKNGIEVPFMREMAGAWQMTVEQKIGLFSAEKKEADPLAASEQSQPRPCAPQVNPHYIWIDFLMQRFEIAKYCSSDQVEIFSSLLQRSLSLNIGGCKGSLNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQTQKLLREDISIMIKFWTAMFSDKKYLTANQLVPPDNQDPSVNSLSVMSDVNRSNLDVTVGSRQQTAQGWINTYPLSSGMSTISKKSGLAKKTNRGTQLHKYYMKRRTLLLSLLATEIERLTTWYNPLSSPELELDQTGENSVANWRSKYISLSEKQWKDNVNLAWSISPHLALQLPTRFKNTEAIGGEVTRLVRLDPGAVSDTPEAIKYLVTWHTIDADAPELSHILCWTPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPFPKGEERKKACLNSLSEVKVQPGCYLPSNPEAIVLDIDYTSGTPLQSAAKAPYLAKFKVKRCGVSELEKEGLSCRSDSIDESEEEGGDGKVCWQAAIFKVGDDCRQVSRISFLRYCK

Target ORF information:

RefSeq Version	XM_012970578.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970578.2

ORF Insert Sequence:

ATGTTGCCTA TCGTCACTTT TGTATGTGAA TCACATGACA ACTGTAGCGG TGTGACGTCG 
GCTGACGGCT CCGGGAGTAA CATGGCGGCC AAGGGGGCTT GCAGCGGGTC GCGGGGGTTT 
TATTTTAACA CTGTGCTGTC GCTGGCCCGT TCCCTGGCAG TGCAGAGGCC TGCTGCATTT 
GAGAAGGTGC AGAAGCTGCT GTGCATGTGC CCTGTCGATT ATCATGGGGT TTTTCAGCTG 
GATGAAAGGC GAAGAGATGC ACTTATTGCT TTAGGAATAT TTCTGGTGGA GTCAGATCTT 
CAGCACAAAG AGACTGTAGT TCCATACCTT TTGAGACTCT TGAGGGGACT TCCAAAGGTG 
CAGTGGATAG AGGAAAGCAA TTCTCGTAAG GGAAAAGGGA CACTTCCAGT GGCAGAAACC 
TTCAGCTTCT GTTTAGTTAC ACTACTGTCG GATGTTGCGC ACAGGGATCT GTCCTTCAGA 
GAGCAGATCT TAGAAGCTGT TTTGGATGTG ATGCAGTGTC TGCTGGGAAT GTGCCAGTCA 
CCCCAAATTC AGGATAAAGA ATACTTGTGT AAATATGCAG TTCCATGCTT GATTGGAATC 
TCACGGGCCT TTGGACGATA CAGCAACACT GAAGAGTCTC TTTTATCCAA GCTGTTCCCC 
AAAGTTTCCC CACAGTCACT AAAGGCGCCA GAGGAATTAG AAGGAATACG TAGACGTTCC 
TTTAATGACT TCCGCTCCAT CCTGCCAAGC TCACTGCTTA CAGTGTGCCA GGGGGATTCT 
CTAAAGCGGA AAGCCAGCAG TGTTTCAAGT GGCTCACAGG TTAGCCCTGA TCGCATTGTT 
CCTCCTCCAG GCTCACCTGC CGGCTCTACT ATTCCTTACT TTGAAGGGTC ATATTTGCCA 
GATGGCAGTG CTGTGGATCC TGACTATTAC CTTACCACCA TCAGCTCTAG TTTCTCAGTC 
TCACCTCTCT TCACTGGTGT CAACAACAAG GAGTTTGATA TGCCCCAAGA TACTTTGCGT 
CAGCTACTCC ATATGGTAAA ACAGATTGTG GCAGACCCAT TCCTGAGTTC TTTGGATATG 
CTCATCTCTG AAGTTACAGA AGCCAATCCT AGTGTGGACC TATATTATAA AACATTCAGT 
GATCCCCTCT ACGTGGCAGT GTTTAAGATG CTCAGAGATA CACTTTATAA TGTTAAAGAT 
CCCCATACTT CATTTGTGAA GGAGATTCAT GACTTTGTGC TTGAACAGTT TAACAGCAGC 
CAGGGTGAGC TGCAGAAAAT TCTACATGAT ATGGATCGAC TGAATAATGA ACTCAGCCCC 
TTGAAATTAC GTTGTCAGGC AAATGCAGCA TGTGTGGACC TTATGGTGTG GGCTGTCAAA 
GATGAACAAG GTGCAGAAAA TCTTTGCATC AAACTATCTG AAAAGCTGCA GTCCAAGACA 
TCTAGCAAAG TGATTATAGC CCATATGCCA CTTCTAATAT GCTGTTTACA GGGGCTAGGG 
CGTCTTTGTG AGCGGTTTCC TGTAGTTGTC CATTCTGTGA CGCCTTCGCT TCGAGATTTT 
CTTGTGATCC CATCTCCGGT GCTGGTGAAG CTTTATAAAT ACCATAGCCA ATATCAGACA 
GGTGCTAATG ATATAAAGAT CAGTGTAACC AATGAGCATT CCGAATCCAC TATGAACATT 
ATGTCTGGCA AGAAAAGCCA GGCATCTATG TATGAACAGC TGCGGGATAT TGCCATAGAT 
AACATCTGCA GGTGTTTAAA AGCTGGGTTA ACCATAGATC CTGTCATTGT TGAAGCTTTT 
CTTGCCAGTT TATCAAATCG CTTGTATATA TCTCAGGAGA ATGAAAAGGA TGCTCATTTG 
ATACCTGATC ATACAATCCG TGCGTTGGGG CACATTGCTG TTGCTCTAAG GGACACCCCC 
AAAGTGATGC AACCCATCCT GCAGATCCTT CAGCAGAAGT TCTGCCAGCC CCCTTCACAG 
CTGGATGTGC TCATTATAGA CCAACTAGGG TGCATGGTGA TCACTGGCAA TCAATATATC 
TATCAAGAAG TGTGGAACCT TTTCCAACAG ATTAGTGTGA AGGCCAGTTC AGTGGTATAC 
TCTGCCACCA AGGACTATAA AGACCATGGC TACAGACACT GCTCCTTGGC TGTGATTAAC 
GCCCTGGCAA ATATTGCAGC CAACATCCAG GGAGAGCACC AGATAGATGA TTTGCTGATG 
AATTTGTTGG AGCTTTTTGT GCAGCTAGGA CTAGAAGGAA AGAGAGCCAG TGAGAGGGCT 
AGCGAAAAGG GGCCTGCATT GAAGGCCTCC AGCAGTGCAG GAAATCTGGG AGTGCTGATA 
CCCGTAATTG CTGTGCTCAC CCGAAGGCTT CCACCAATAA AAGAGGCCAA GCCACGACTT 
CAGAAACTCT TCAGAGATTT TTGGTTGTAC TCTGTGCTGA TGGGTTTTGC AGTGGAAGGA 
TCAGGTCTCT GGCCTGAGGA ATGGTATGAA GGTGTCTGTG AAATTGCCAC TAAATCTCCT 
TTGCTGACGT TCCCCAGCAA AGAGCCTCTG CGCTCTGTAC TGCAGTACAA CTCTGCCATG 
AAAAATGACA CTGTAACGCC AGCAGAACTT AATGAGCTGA GAAGCACAAT TATCAACCTT 
CTTGATCCTG CTCCGGAAGT GTCTGCACTC ATCAACAAAC TGGACTTTGC CATGTCTACC 
TACCTGCTCT CTGTGTACCG CCTGGAGCAC ATGAGGGTTC TGCATGCCAC TGATAAGGAT 
CGATTTCAAG TTATGTTTTT TTACTTCGAG GATAAAGCTA TCCAAAAGGA TAAATCAGGT 
ATGATGCAGT GTGTTATTGC AGTAGCAGAC AAAGTGTTTG AAGCTTTTCT GAACGTGATG 
GCTGATAAGC CTAAAACGAA AGAGAACGAG GAAGAATTGG AGCGCCATGC ACAATTCCTG 
CTGGTAAACT TTAATCACAT CCACAAGCGC ATTCGACGGG TCGCTGACAA ATACTTGTCA 
GGGCTAGTGG ACAAATTTCC CCATTTGCTG TGGAGTGGTA CTGTGCTGAA AACCATGCTG 
GACATACTGC AGACTCTTTC CTTGTCTCTT AGTGCAGACA TTCACAAAGA CCAGCCTTAT 
TATGACATTC CAGATGCACC TTATAGAATA ACCGTTCCCG ATACCTATGA GGCCAGAGAG 
AGCATTGTCA AAGACTTTGC AGCTCGATGT GGCATGATTC TTCAGGAAGC AATGAAATGG 
GCTCCTTCAG TGACCAAGTC CCACCTGCAG GAATATCTAA ACAAGCACCA GAACTGGATG 
TCTGGCCTTT CCCAGCACAC TGGTCTTGCC ATGGCTACTG AAAGCATACT GCATTTTGCT 
GGGTACAACA GGCAGAATAC TACCTTGGGG GTGACACAAC TAACAGAAAG GCCAGCCTGT 
GTCAAGAAGG ATTATTCAAA CTTCATGGCA TCTCTCAACC TGCGCAACCG TTATGCTGGA 
GAGGTTGCAG GCATGATTGA GTTTTCTGAT GCCACTGGCC GAACTTCTGA CTTAAATAGG 
CTCATGCTGA AGCACCTGGG CGATGCCTTG GACTGCGGCC AACCAGAGTC TTATACACAG 
GTCATGTTCA AACTTACTGC GCTGCTCATT AGCAGCAAAG AGTGTGATGC TCAGCTTCTG 
CACCACCTGT GCTGGGCACC CCTGCGGATG TTCACTGATC ATGGTATGGA GACCGCTATT 
GCCTGCTGGG ACTGGTTGCT TGCAGCTAAA AATGGCATTG AAGTACCGTT TATGCGTGAA 
ATGGCTGGTG CTTGGCAAAT GACTGTAGAG CAAAAGATTG GACTTTTCTC AGCAGAAAAG 
AAGGAGGCAG ATCCTCTTGC TGCATCTGAA CAGAGCCAAC CACGTCCATG TGCACCACAA 
GTGAACCCAC ATTATATTTG GATTGATTTT CTCATGCAAA GGTTTGAGAT TGCCAAATAC 
TGCAGCTCTG ATCAGGTGGA AATCTTCTCC AGCCTTCTGC AGCGCTCCCT GTCCCTAAAC 
ATTGGTGGTT GTAAAGGAAG TCTTAACCGA CATGTGGCAG CTATTGGCCC TCGATTTAAG 
TTGCTGACTC TCGGGCTGTC CCTGTTACAT GCAGATGTGG TCCCAAATGC GACAATCCGG 
AATGTCCTTA GAGAAAAGAT CTACTCTACA GCATTTGATT ATTTTAGTTG CCCTCCTAAG 
TTCCCGACTC AGACGCAGAA GCTGCTAAGG GAGGATATCA GCATCATGAT CAAGTTCTGG 
ACAGCCATGT TTTCTGATAA AAAATATCTC ACTGCCAATC AGCTAGTACC CCCAGATAAT 
CAAGACCCTT CTGTAAACAG CTTGTCTGTG ATGTCAGATG TGAACCGCAG CAATTTGGAT 
GTGACTGTTG GCTCCCGACA GCAAACAGCT CAGGGCTGGA TCAATACATA CCCTCTGTCC 
AGTGGAATGT CCACCATCTC TAAAAAATCT GGTTTAGCAA AGAAAACAAA CCGTGGGACT 
CAGCTGCACA AGTACTATAT GAAGAGGCGG ACTCTGTTGC TGTCACTTTT GGCCACTGAG 
ATTGAACGCC TGACCACATG GTACAACCCC TTGTCCTCCC CTGAGTTGGA ACTGGATCAG 
ACAGGTGAAA ACAGTGTGGC TAACTGGAGA TCTAAGTACA TTAGTTTGAG TGAGAAGCAG 
TGGAAGGACA ATGTGAACTT AGCATGGAGC ATCTCCCCTC ACTTGGCTTT GCAATTACCC 
ACCAGGTTCA AGAACACTGA GGCTATTGGG GGAGAAGTGA CCCGCCTGGT TAGATTGGAC 
CCAGGAGCAG TGAGTGACAC TCCAGAAGCA ATAAAGTACT TGGTAACATG GCACACAATA 
GATGCTGATG CTCCTGAACT TAGTCACATA CTCTGCTGGA CACCGACTGA CCCACCTACA 
GGACTCTCCT ACTTCTCTAG CATGTACCCA CCACATCCAC TTACTGCGCA ATATGGCGTG 
AAAGTGCTGC GATCATTCCC ACCGGATGCA ATCTTGTTCT ACATTCCGCA GATAGTTCAA 
GCCTTGAGAT ATGATAAGAT GGGTTACGTT CGAGAATACA TTCTATGGGC AGCTGCCAAA 
TCACAGCTTT TGGCTCATCA GTTTATTTGG AACATGAAGA CAAATATTTT CCTTGATGAG 
GAAGGGCACC AAAAAGACCC TGATATTGGA GAACTATTGG AGCAGCTGGT TGAAGAAATT 
ACAGGATCTC TGTCTGGGCC AGCCAAGGAT TTCTACCAGC GAGAATTTGA CTTTTTCAAT 
AAGATCACAA ATGTCTCTGC CATCATTAAG CCATTTCCAA AAGGAGAAGA ACGGAAAAAG 
GCCTGTCTGA ACTCTCTTTC AGAAGTAAAA GTGCAGCCAG GTTGTTACCT GCCCAGTAAT 
CCAGAAGCCA TTGTGTTGGA CATTGATTAC ACATCTGGGA CCCCTTTGCA GAGTGCTGCG 
AAGGCTCCTT ATTTGGCCAA ATTTAAGGTG AAGCGATGTG GAGTAAGTGA ACTGGAGAAG 
GAAGGTCTCA GCTGCCGTTC AGATTCTATT GATGAGAGTG AGGAGGAAGG TGGAGATGGG 
AAGGTTTGCT GGCAAGCGGC TATCTTTAAG GTCGGAGATG ACTGCAGACA GGTGAGCAGA 
ATATCTTTTC TGCGTTACTG TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pi4ka ( XM_031905856.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761716.1 Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X1, mRNA.

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CloneID	OXa47456
Clone ID Related Accession (Same CDS sequence)	XM_031905856.1
Accession Version	XM_031905856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphatidylinositol 4-kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA AGGGGGCTTG CAGCGGGTCG CGGGGGTTTT ATTTTAACAC TGTGCTGTCG 
CTGGCCCGTT CCCTGGCAGT GCAGAGGCCT GCTGCATTTG AGAAGGTGCA GAAGCTGCTG 
TGCATGTGCC CTGTCGATTA TCATGGGGTT TTTCAGCTGG ATGAAAGGCG AAGAGATGCA 
CTTATTGCTT TAGGAATATT TCTGGTGGAG TCAGATCTTC AGCACAAAGA GACTGTAGTT 
CCATACCTTT TGAGACTCTT GAGGGGACTT CCAAAGGTGC AGTGGATAGA GGAAAGCAAT 
TCTCGTAAGG GAAAAGGGAC GCTTCCAGTG GCAGAAACCT TCAGCTTCTG TTTAGTTACA 
CTACTGTCGG ATGTTGCGCA CAGGGATCTG TCCTTCAGAG AGCAGATCTT AGAAGCTGTT 
TTGGATGTGA TGCAGTGTCT GCTGGGAATG TGCCAGTCAC CCCAAATTCA GGATAAAGAA 
TACTTGTGTA AATATGCAGT TCCATGCTTG ATTGGAATCT CACGGGCCTT TGGACGATAC 
AGCAACACTG AAGAGTCTCT TTTATCCAAG CTGTTCCCCA AAGTTTCCCC ACAGTCACTA 
AAGGCGCCAG AGGAATTAGA AGGAATACGT AGACGTTCCT TTAATGACTT CCGCTCCATC 
CTGCCAAGCT CACTGCTTAC AGTGTGCCAG GGGGATTCTC TAAAGCGGAA AGCCAGCAGT 
GTTTCAAGTG GCTCACAGGT TAGCCCTGAT CGCATTGTTC CTCCTCCAGG CTCACCTGCC 
GGCTCTACTA TTCCTTACTT TGAAGGGTCA TATTTGCCAG ATGGCAGTGC TGTGGATCCT 
GACTATTACC TTACCACCAT CAGCTCTAGT TTCTCAGTCT CACCTCTCTT CACTGGTGTC 
AACAACAAGG AGTTTGATAT GCCCCAAGAT ACTTTGCGTC AGCTACTCCA TATGGTGAAA 
CAGATTGTGG CAGACCCATT CCTGAGTTCT TTGGATATGC TCATCTCTGA AGTTACAGAA 
GCCAATCCTA GTGTGGACCT ATATTATAAA ACATTCAGTG ATCCCCTCTA CGTGGCAGTG 
TTTAAGATGC TCAGAGATAC ACTTTATAAT GTTAAAGATC CCCATACTTC ATTTGTGAAG 
GAGATTCATG ACTTTGTGCT TGAACAGTTT AACAGCAGCC AGGGTGAGCT GCAGAAAATT 
CTACATGATA TGGATCGACT GAATAATGAA CTCAGCCCCT TGAAATTACG TTGTCAGGCA 
AATGCAGCAT GTGTGGACCT TATGGTGTGG GCTGTCAAAG ATGAACAAGG TGCAGAAAAT 
CTTTGCATCA AACTATCTGA AAAGCTGCAG TCCAAGACAT CTAGCAAAGT GATTATAGCC 
CATATGCCAC TTCTAATATG CTGTTTACAG GGGCTAGGGC GTCTTTGTGA GCGGTTTCCT 
GTAGTTGTCC ATTCTGTGAC GCCTTCGCTT CGAGATTTTC TTGTTATCCC ATCTCCGGTG 
CTGGTGAAGC TTTATAAATA CCATAGCCAA TATCAGACAG GTGCTAATGA TATAAAGATC 
AGTGTAACCA ATGAGCATTC CGAATCCACT ATGAACATTA TGTCTGGCAA GAAAAGCCAG 
GCATCTATGT ATGAACAGCT ACGGGATATT GCCATAGATA ACATCTGCAG GTGTTTAAAA 
GCTGGGTTAA CCATAGATCC TGTCATTGTT GAAGCTTTTC TTGCCAGTTT ATCAAATCGC 
TTGTATATAT CTCAGGAGAA TGAAAAGGAT GCTCATTTGA TACCTGATCA TACAATCCGT 
GCGTTGGGGC ACATTGCTGT TGCTCTAAGG GACACCCCCA AAGTGATGCA ACCCATCCTG 
CAGATCCTTC AGCAGAAGTT CTGCCAGCCC CCTTCACAGC TGGATGTGCT CATTATAGAC 
CAACTAGGGT GCATGGTGAT CACTGGCAAT CAATATATCT ATCAAGAAGT GTGGAACCTT 
TTCCAGCAGA TTAGTGTGAA GGCCAGTTCA GTGGTATACT CTGCCACCAA GGACTATAAA 
GACCATGGCT ACAGACACTG CTCCTTGGCT GTGATTAACG CCCTGGCAAA TATTGCAGCC 
AACATCCAGG GAGAGCACCA GATAGATGAT TTGCTGATGA ATTTGTTGGA GCTTTTTGTG 
CAGCTAGGAC TAGAAGGAAA GAGAGCCAGT GAGAGGGCTA GCGAAAAGGG GCCTGCATTG 
AAGGCCTCCA GCAGTGCAGG AAATCTGGGA GTGCTGATAC CCGTAATTGC TGTGCTCACC 
CGAAGGCTTC CACCAATAAA AGAGGCCAAG CCACGACTTC AGAAACTCTT CAGAGATTTT 
TGGTTGTACT CTGTGCTGAT GGGTTTTGCA GTGGAAGGAT CAGGTCTCTG GCCTGAGGAA 
TGGTATGAAG GTGTCTGTGA AATTGCCACT AAATCTCCTT TGCTGACGTT CCCCAGCAAA 
GAGCCTCTGC GCTCTGTACT GCAGTACAAC TCTGCCATGA AAAATGACAC TGTAACGCCA 
GCAGAACTTA ATGAGCTGAG AAGCACAATT ATCAACCTTC TTGATCCTGC TCCGGAAGTG 
TCTGCACTCA TCAACAAACT GGACTTTGCC ATGTCTACCT ACCTGCTCTC TGTGTACCGC 
CTGGAGCACA TGAGGGTTCT GCATGCCACT GATAAGGATC GATTTCAAGT TATGTTTTTT 
TACTTCGAGG ATAAAGCTAT CCAAAAGGAT AAATCAGGTA TGATGCAGTG TGTTATTGCA 
GTAGCAGACA AAGTGTTTGA AGCTTTTCTG AACGTGATGG CTGATAAGCC TAAAACAAAG 
GAGAACGAGG AAGAATTGGA GCGCCATGCA CAATTCCTGC TGGTAAACTT TAATCACATC 
CACAAGCGCA TTCGACGGGT CGCTGACAAA TACTTGTCAG GGCTAGTGGA CAAATTTCCC 
CATTTGCTGT GGAGTGGTAC TGTGCTGAAA ACCATGCTGG ACATACTGCA GACTCTTTCC 
TTGTCTCTTA GTGCAGACAT TCACAAAGAC CAGCCTTATT ATGACATTCC AGATGCACCT 
TATAGAATAA CCGTTCCCGA TACCTATGAG GCCAGAGAGA GCATTGTCAA AGACTTTGCA 
GCTCGATGTG GCATGATTCT TCAGGAAGCA ATGAAATGGG CTCCTTCAGT GACCAAGTCC 
CACCTGCAGG AATATCTAAA CAAGCACCAG AACTGGATGT CTGGCCTTTC CCAGCACACT 
GGTCTTGCCA TGGCTACTGA AAGCATACTG CATTTTGCTG GGTACAACAG GCAGAATACT 
ACCTTGGGGT CTCCAGCTTC CTCTGCCCAG GTGACACAAC TAACAGAAAG GCCAGCCTGT 
GTCAAGAAGG ATTATTCAAA CTTCATGGCA TCTCTCAACC TGCGCAACCG TTATGCTGGA 
GAGGTTGCAG GCATGATTGA GTTTTCTGAT GCCACTGGCC GAACTTCTGA CTTAAATAGG 
CTCATGCTGA AGCACCTGGG CGATGCCTTG GACTGCGGCC AACCAGAGTC TTATACACAG 
GTCATGTTCA AACTTACTGC GCTGCTCATT AGCAGCAAAG AGTGTGATGC TCAGCTTCTG 
CACCACCTGT GCTGGGCACC CCTGCGGATG TTCACTGATC ATGGTATGGA GACCGCTATT 
GCCTGCTGGG ACTGGTTGCT TGCAGCTAAA AATGGCATTG AAGTACCGTT TATGCGTGAA 
ATGGCTGGTG CTTGGCAAAT GACTGTAGAG CAAAAGATTG GACTTTTCTC AGCAGAAAAG 
AAGGAGGCAG ATCCTCTTGC TGCATCTGAA CAGAGCCAAC CACGTCCATG TGCACCACAA 
GTGAACCCAC ATTATATTTG GATTGATTTT CTCATGCAAA GGTTTGAGAT TGCCAAATAC 
TGCAGCTCTG ATCAGGTGGA AATCTTCTCC AGCCTTCTGC AGCGCTCCCT GTCCCTAAAC 
ATTGGTGGTT GTAAAGGAAG TCTTAACCGA CATGTGGCAG CTATTGGCCC TCGATTTAAG 
TTGCTGACTC TCGGGCTGTC CCTGTTACAT GCAGACGTGG TCCCAAATGC GACAATCCGG 
AATGTCCTTA GAGAAAAGAT CTACTCTACA GCATTTGATT ATTTTAGTTG CCCTCCTAAG 
TTCCCGACTC AGACGCAGAA GCTGCTAAGG GAGGATATCA GCATCATGAT CAAGTTCTGG 
ACAGCCATGT TTTCTGATAA AAAATATCTC ACTGCCAATC AGCTAGTACC CCCAGATAAT 
CAAGACCCTT CTGTAAACAG CTTGTCTGTG ATGTCAGATG TGAACCGCAG CAATTTGGAT 
GTGACTGTTG GCTCCCGACA GCAAACAGCT CAGGGCTGGA TCAATACATA CCCTCTGTCC 
AGTGGAATGT CCACCATCTC TAAAAAATCT GGTTTAGCAA AGAAAACAAA CCGTGGGACT 
CAGCTGCACA AGTACTATAT GAAGAGGCGG ACTCTGTTGC TGTCACTTTT GGCCACTGAG 
ATTGAACGCC TGACCACATG GTACAACCCC TTGTCCTCCC CTGAGTTGGA ACTGGATCAG 
ACAGGTGAAA ACAGTGTGGC TAACTGGAGA TCTAAGTACA TTAGTTTGAG TGAGAAGCAG 
TGGAAGGACA ATGTGAACTT AGCATGGAGC ATCTCCCCTC ACTTGGCTTT GCAATTACCC 
ACCAGGTTCA AGAACACTGA GGCTATTGGG GGAGAAGTGA CCCGCCTGGT TAGATTGGAC 
CCAGGAGCAG TGAGTGACAC TCCAGAAGCA ATAAAGTACT TGGTAACATG GCACACAATA 
GATGCTGATG CTCCTGAACT TAGTCACATA CTCTGCTGGA CACCGACTGA CCCACCTACA 
GGACTCTCCT ACTTCTCTAG CATGTACCCA CCACATCCAC TTACTGCGCA ATATGGCGTG 
AAAGTGCTGC GATCATTCCC ACCGGATGCA ATCTTGTTCT ACATTCCGCA GATAGTTCAA 
GCCTTGAGAT ATGATAAGAT GGGTTACGTT CGAGAATACA TTCTATGGGC AGCTGCCAAA 
TCACAGCTTT TGGCTCATCA GTTTATTTGG AACATGAAGA CAAATATTTT CCTTGATGAG 
GAAGGGCACC AAAAAGACCC TGATATTGGA GAACTATTGG AGCAGCTGGT TGAAGAAATT 
ACAGGATCTC TGTCTGGGCC AGCCAAGGAT TTCTACCAGC GAGAATTTGA CTTTTTCAAT 
AAGATCACAA ATGTCTCTGC CATCATTAAG CCATTTCCAA AAGGAGAAGA ACGGAAAAAG 
GCCTGTCTGA ACTCTCTTTC AGAAGTAAAA GTGCAGCCAG GTTGTTACCT GCCCAGTAAT 
CCAGAAGCCA TTGTGTTGGA CATTGATTAC ACATCTGGGA CCCCTTTGCA GAGTGCTGCG 
AAGGCTCCTT ATTTGGCCAA ATTTAAGGTG AAGCGATGTG GAGTAAGTGA ACTGGAGAAG 
GAAGGTCTCA GCTGCCGTTC AGATTCTATT GATGAGAGTG AGGAGGAAGG TGGAGATGGG 
AAGGTTTGCT GGCAAGCGGC TATCTTTAAG GTCGGAGATG ACTGCAGACA GGATATGCTG 
GCTCTGCAGA TCATTGGTCT CTTTAAGAAC ATTTTCCAGC TGGTGGGACT TGATCTCTTT 
GTTTTCCCAT ACAGAGTGGT AGCAACAGCT CCAGGGTGCG GAGTTATTGA ATGTATACCA 
GACTGCACCT CGCGGGACCA GCTCGGTCGC CAAACAGATT TTGGAATGTA TGACTATTTT 
ACTCGGCAGT ATGGGGATGA ATCAGCTCTT GCTTTCCAAA AGGCTCGATA TAACTTTATC 
CGAAGTATGG CTGCCTACAG CCTCCTGCTG TTCCTGCTCC AGATTAAAGA TCGACATAAT 
GGAAATATCA TGTTGGATAA AAAGGGGCAC ATTATCCACA TTGATTTTGG TTTCATGTTT 
GAAAGCTCCC CAGGGGGCAA CCTGGGATGG GAGCCAGATA TAAAGCTTAC TGATGAGATG 
GTGATGATTA TGGGGGGCAA GATGGAAGCT ACACCTTTCA AATGGTTCAT GGAGATGTGC 
GTGCGTGGCT ACTTAGCTGT AAGGCCGTAC ATGGATGCTG TGGTGTCCCT AGTCACTCTT 
ATGCTGGACA CAGGATTGCC ATGTTTCCGT GGACAAACCA TTAAACTACT GAAGCAGAGG 
TTCAGCCCCA ACTCAACAGA GAGGGAAGCA GCCAATTTCA TCTTAAAGGT CATTCAAAAT 
TGCTTCCTCA GCAACAGGAG CCGAACCTAC GATATGATCC AGTATTATCA GAATGACATC 
CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761716.1
CDS	21..6329
Translation	MAAKGACSGSRGFYFNTVLSLARSLAVQRPAAFEKVQKLLCMCPVDYHGVFQLDERRRDALIALGIFLVESDLQHKETVVPYLLRLLRGLPKVQWIEESNSRKGKGTLPVAETFSFCLVTLLSDVAHRDLSFREQILEAVLDVMQCLLGMCQSPQIQDKEYLCKYAVPCLIGISRAFGRYSNTEESLLSKLFPKVSPQSLKAPEELEGIRRRSFNDFRSILPSSLLTVCQGDSLKRKASSVSSGSQVSPDRIVPPPGSPAGSTIPYFEGSYLPDGSAVDPDYYLTTISSSFSVSPLFTGVNNKEFDMPQDTLRQLLHMVKQIVADPFLSSLDMLISEVTEANPSVDLYYKTFSDPLYVAVFKMLRDTLYNVKDPHTSFVKEIHDFVLEQFNSSQGELQKILHDMDRLNNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHMPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYQTGANDIKISVTNEHSESTMNIMSGKKSQASMYEQLRDIAIDNICRCLKAGLTIDPVIVEAFLASLSNRLYISQENEKDAHLIPDHTIRALGHIAVALRDTPKVMQPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQGEHQIDDLLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELNELRSTIINLLDPAPEVSALINKLDFAMSTYLLSVYRLEHMRVLHATDKDRFQVMFFYFEDKAIQKDKSGMMQCVIAVADKVFEAFLNVMADKPKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPSVTKSHLQEYLNKHQNWMSGLSQHTGLAMATESILHFAGYNRQNTTLGSPASSAQVTQLTERPACVKKDYSNFMASLNLRNRYAGEVAGMIEFSDATGRTSDLNRLMLKHLGDALDCGQPESYTQVMFKLTALLISSKECDAQLLHHLCWAPLRMFTDHGMETAIACWDWLLAAKNGIEVPFMREMAGAWQMTVEQKIGLFSAEKKEADPLAASEQSQPRPCAPQVNPHYIWIDFLMQRFEIAKYCSSDQVEIFSSLLQRSLSLNIGGCKGSLNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQTQKLLREDISIMIKFWTAMFSDKKYLTANQLVPPDNQDPSVNSLSVMSDVNRSNLDVTVGSRQQTAQGWINTYPLSSGMSTISKKSGLAKKTNRGTQLHKYYMKRRTLLLSLLATEIERLTTWYNPLSSPELELDQTGENSVANWRSKYISLSEKQWKDNVNLAWSISPHLALQLPTRFKNTEAIGGEVTRLVRLDPGAVSDTPEAIKYLVTWHTIDADAPELSHILCWTPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPFPKGEERKKACLNSLSEVKVQPGCYLPSNPEAIVLDIDYTSGTPLQSAAKAPYLAKFKVKRCGVSELEKEGLSCRSDSIDESEEEGGDGKVCWQAAIFKVGDDCRQDMLALQIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESALAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKQRFSPNSTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	XM_031905856.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905856.1

ORF Insert Sequence:

ATGGCGGCCA AGGGGGCTTG CAGCGGGTCG CGGGGGTTTT ATTTTAACAC TGTGCTGTCG 
CTGGCCCGTT CCCTGGCAGT GCAGAGGCCT GCTGCATTTG AGAAGGTGCA GAAGCTGCTG 
TGCATGTGCC CTGTCGATTA TCATGGGGTT TTTCAGCTGG ATGAAAGGCG AAGAGATGCA 
CTTATTGCTT TAGGAATATT TCTGGTGGAG TCAGATCTTC AGCACAAAGA GACTGTAGTT 
CCATACCTTT TGAGACTCTT GAGGGGACTT CCAAAGGTGC AGTGGATAGA GGAAAGCAAT 
TCTCGTAAGG GAAAAGGGAC GCTTCCAGTG GCAGAAACCT TCAGCTTCTG TTTAGTTACA 
CTACTGTCGG ATGTTGCGCA CAGGGATCTG TCCTTCAGAG AGCAGATCTT AGAAGCTGTT 
TTGGATGTGA TGCAGTGTCT GCTGGGAATG TGCCAGTCAC CCCAAATTCA GGATAAAGAA 
TACTTGTGTA AATATGCAGT TCCATGCTTG ATTGGAATCT CACGGGCCTT TGGACGATAC 
AGCAACACTG AAGAGTCTCT TTTATCCAAG CTGTTCCCCA AAGTTTCCCC ACAGTCACTA 
AAGGCGCCAG AGGAATTAGA AGGAATACGT AGACGTTCCT TTAATGACTT CCGCTCCATC 
CTGCCAAGCT CACTGCTTAC AGTGTGCCAG GGGGATTCTC TAAAGCGGAA AGCCAGCAGT 
GTTTCAAGTG GCTCACAGGT TAGCCCTGAT CGCATTGTTC CTCCTCCAGG CTCACCTGCC 
GGCTCTACTA TTCCTTACTT TGAAGGGTCA TATTTGCCAG ATGGCAGTGC TGTGGATCCT 
GACTATTACC TTACCACCAT CAGCTCTAGT TTCTCAGTCT CACCTCTCTT CACTGGTGTC 
AACAACAAGG AGTTTGATAT GCCCCAAGAT ACTTTGCGTC AGCTACTCCA TATGGTGAAA 
CAGATTGTGG CAGACCCATT CCTGAGTTCT TTGGATATGC TCATCTCTGA AGTTACAGAA 
GCCAATCCTA GTGTGGACCT ATATTATAAA ACATTCAGTG ATCCCCTCTA CGTGGCAGTG 
TTTAAGATGC TCAGAGATAC ACTTTATAAT GTTAAAGATC CCCATACTTC ATTTGTGAAG 
GAGATTCATG ACTTTGTGCT TGAACAGTTT AACAGCAGCC AGGGTGAGCT GCAGAAAATT 
CTACATGATA TGGATCGACT GAATAATGAA CTCAGCCCCT TGAAATTACG TTGTCAGGCA 
AATGCAGCAT GTGTGGACCT TATGGTGTGG GCTGTCAAAG ATGAACAAGG TGCAGAAAAT 
CTTTGCATCA AACTATCTGA AAAGCTGCAG TCCAAGACAT CTAGCAAAGT GATTATAGCC 
CATATGCCAC TTCTAATATG CTGTTTACAG GGGCTAGGGC GTCTTTGTGA GCGGTTTCCT 
GTAGTTGTCC ATTCTGTGAC GCCTTCGCTT CGAGATTTTC TTGTTATCCC ATCTCCGGTG 
CTGGTGAAGC TTTATAAATA CCATAGCCAA TATCAGACAG GTGCTAATGA TATAAAGATC 
AGTGTAACCA ATGAGCATTC CGAATCCACT ATGAACATTA TGTCTGGCAA GAAAAGCCAG 
GCATCTATGT ATGAACAGCT ACGGGATATT GCCATAGATA ACATCTGCAG GTGTTTAAAA 
GCTGGGTTAA CCATAGATCC TGTCATTGTT GAAGCTTTTC TTGCCAGTTT ATCAAATCGC 
TTGTATATAT CTCAGGAGAA TGAAAAGGAT GCTCATTTGA TACCTGATCA TACAATCCGT 
GCGTTGGGGC ACATTGCTGT TGCTCTAAGG GACACCCCCA AAGTGATGCA ACCCATCCTG 
CAGATCCTTC AGCAGAAGTT CTGCCAGCCC CCTTCACAGC TGGATGTGCT CATTATAGAC 
CAACTAGGGT GCATGGTGAT CACTGGCAAT CAATATATCT ATCAAGAAGT GTGGAACCTT 
TTCCAGCAGA TTAGTGTGAA GGCCAGTTCA GTGGTATACT CTGCCACCAA GGACTATAAA 
GACCATGGCT ACAGACACTG CTCCTTGGCT GTGATTAACG CCCTGGCAAA TATTGCAGCC 
AACATCCAGG GAGAGCACCA GATAGATGAT TTGCTGATGA ATTTGTTGGA GCTTTTTGTG 
CAGCTAGGAC TAGAAGGAAA GAGAGCCAGT GAGAGGGCTA GCGAAAAGGG GCCTGCATTG 
AAGGCCTCCA GCAGTGCAGG AAATCTGGGA GTGCTGATAC CCGTAATTGC TGTGCTCACC 
CGAAGGCTTC CACCAATAAA AGAGGCCAAG CCACGACTTC AGAAACTCTT CAGAGATTTT 
TGGTTGTACT CTGTGCTGAT GGGTTTTGCA GTGGAAGGAT CAGGTCTCTG GCCTGAGGAA 
TGGTATGAAG GTGTCTGTGA AATTGCCACT AAATCTCCTT TGCTGACGTT CCCCAGCAAA 
GAGCCTCTGC GCTCTGTACT GCAGTACAAC TCTGCCATGA AAAATGACAC TGTAACGCCA 
GCAGAACTTA ATGAGCTGAG AAGCACAATT ATCAACCTTC TTGATCCTGC TCCGGAAGTG 
TCTGCACTCA TCAACAAACT GGACTTTGCC ATGTCTACCT ACCTGCTCTC TGTGTACCGC 
CTGGAGCACA TGAGGGTTCT GCATGCCACT GATAAGGATC GATTTCAAGT TATGTTTTTT 
TACTTCGAGG ATAAAGCTAT CCAAAAGGAT AAATCAGGTA TGATGCAGTG TGTTATTGCA 
GTAGCAGACA AAGTGTTTGA AGCTTTTCTG AACGTGATGG CTGATAAGCC TAAAACAAAG 
GAGAACGAGG AAGAATTGGA GCGCCATGCA CAATTCCTGC TGGTAAACTT TAATCACATC 
CACAAGCGCA TTCGACGGGT CGCTGACAAA TACTTGTCAG GGCTAGTGGA CAAATTTCCC 
CATTTGCTGT GGAGTGGTAC TGTGCTGAAA ACCATGCTGG ACATACTGCA GACTCTTTCC 
TTGTCTCTTA GTGCAGACAT TCACAAAGAC CAGCCTTATT ATGACATTCC AGATGCACCT 
TATAGAATAA CCGTTCCCGA TACCTATGAG GCCAGAGAGA GCATTGTCAA AGACTTTGCA 
GCTCGATGTG GCATGATTCT TCAGGAAGCA ATGAAATGGG CTCCTTCAGT GACCAAGTCC 
CACCTGCAGG AATATCTAAA CAAGCACCAG AACTGGATGT CTGGCCTTTC CCAGCACACT 
GGTCTTGCCA TGGCTACTGA AAGCATACTG CATTTTGCTG GGTACAACAG GCAGAATACT 
ACCTTGGGGT CTCCAGCTTC CTCTGCCCAG GTGACACAAC TAACAGAAAG GCCAGCCTGT 
GTCAAGAAGG ATTATTCAAA CTTCATGGCA TCTCTCAACC TGCGCAACCG TTATGCTGGA 
GAGGTTGCAG GCATGATTGA GTTTTCTGAT GCCACTGGCC GAACTTCTGA CTTAAATAGG 
CTCATGCTGA AGCACCTGGG CGATGCCTTG GACTGCGGCC AACCAGAGTC TTATACACAG 
GTCATGTTCA AACTTACTGC GCTGCTCATT AGCAGCAAAG AGTGTGATGC TCAGCTTCTG 
CACCACCTGT GCTGGGCACC CCTGCGGATG TTCACTGATC ATGGTATGGA GACCGCTATT 
GCCTGCTGGG ACTGGTTGCT TGCAGCTAAA AATGGCATTG AAGTACCGTT TATGCGTGAA 
ATGGCTGGTG CTTGGCAAAT GACTGTAGAG CAAAAGATTG GACTTTTCTC AGCAGAAAAG 
AAGGAGGCAG ATCCTCTTGC TGCATCTGAA CAGAGCCAAC CACGTCCATG TGCACCACAA 
GTGAACCCAC ATTATATTTG GATTGATTTT CTCATGCAAA GGTTTGAGAT TGCCAAATAC 
TGCAGCTCTG ATCAGGTGGA AATCTTCTCC AGCCTTCTGC AGCGCTCCCT GTCCCTAAAC 
ATTGGTGGTT GTAAAGGAAG TCTTAACCGA CATGTGGCAG CTATTGGCCC TCGATTTAAG 
TTGCTGACTC TCGGGCTGTC CCTGTTACAT GCAGACGTGG TCCCAAATGC GACAATCCGG 
AATGTCCTTA GAGAAAAGAT CTACTCTACA GCATTTGATT ATTTTAGTTG CCCTCCTAAG 
TTCCCGACTC AGACGCAGAA GCTGCTAAGG GAGGATATCA GCATCATGAT CAAGTTCTGG 
ACAGCCATGT TTTCTGATAA AAAATATCTC ACTGCCAATC AGCTAGTACC CCCAGATAAT 
CAAGACCCTT CTGTAAACAG CTTGTCTGTG ATGTCAGATG TGAACCGCAG CAATTTGGAT 
GTGACTGTTG GCTCCCGACA GCAAACAGCT CAGGGCTGGA TCAATACATA CCCTCTGTCC 
AGTGGAATGT CCACCATCTC TAAAAAATCT GGTTTAGCAA AGAAAACAAA CCGTGGGACT 
CAGCTGCACA AGTACTATAT GAAGAGGCGG ACTCTGTTGC TGTCACTTTT GGCCACTGAG 
ATTGAACGCC TGACCACATG GTACAACCCC TTGTCCTCCC CTGAGTTGGA ACTGGATCAG 
ACAGGTGAAA ACAGTGTGGC TAACTGGAGA TCTAAGTACA TTAGTTTGAG TGAGAAGCAG 
TGGAAGGACA ATGTGAACTT AGCATGGAGC ATCTCCCCTC ACTTGGCTTT GCAATTACCC 
ACCAGGTTCA AGAACACTGA GGCTATTGGG GGAGAAGTGA CCCGCCTGGT TAGATTGGAC 
CCAGGAGCAG TGAGTGACAC TCCAGAAGCA ATAAAGTACT TGGTAACATG GCACACAATA 
GATGCTGATG CTCCTGAACT TAGTCACATA CTCTGCTGGA CACCGACTGA CCCACCTACA 
GGACTCTCCT ACTTCTCTAG CATGTACCCA CCACATCCAC TTACTGCGCA ATATGGCGTG 
AAAGTGCTGC GATCATTCCC ACCGGATGCA ATCTTGTTCT ACATTCCGCA GATAGTTCAA 
GCCTTGAGAT ATGATAAGAT GGGTTACGTT CGAGAATACA TTCTATGGGC AGCTGCCAAA 
TCACAGCTTT TGGCTCATCA GTTTATTTGG AACATGAAGA CAAATATTTT CCTTGATGAG 
GAAGGGCACC AAAAAGACCC TGATATTGGA GAACTATTGG AGCAGCTGGT TGAAGAAATT 
ACAGGATCTC TGTCTGGGCC AGCCAAGGAT TTCTACCAGC GAGAATTTGA CTTTTTCAAT 
AAGATCACAA ATGTCTCTGC CATCATTAAG CCATTTCCAA AAGGAGAAGA ACGGAAAAAG 
GCCTGTCTGA ACTCTCTTTC AGAAGTAAAA GTGCAGCCAG GTTGTTACCT GCCCAGTAAT 
CCAGAAGCCA TTGTGTTGGA CATTGATTAC ACATCTGGGA CCCCTTTGCA GAGTGCTGCG 
AAGGCTCCTT ATTTGGCCAA ATTTAAGGTG AAGCGATGTG GAGTAAGTGA ACTGGAGAAG 
GAAGGTCTCA GCTGCCGTTC AGATTCTATT GATGAGAGTG AGGAGGAAGG TGGAGATGGG 
AAGGTTTGCT GGCAAGCGGC TATCTTTAAG GTCGGAGATG ACTGCAGACA GGATATGCTG 
GCTCTGCAGA TCATTGGTCT CTTTAAGAAC ATTTTCCAGC TGGTGGGACT TGATCTCTTT 
GTTTTCCCAT ACAGAGTGGT AGCAACAGCT CCAGGGTGCG GAGTTATTGA ATGTATACCA 
GACTGCACCT CGCGGGACCA GCTCGGTCGC CAAACAGATT TTGGAATGTA TGACTATTTT 
ACTCGGCAGT ATGGGGATGA ATCAGCTCTT GCTTTCCAAA AGGCTCGATA TAACTTTATC 
CGAAGTATGG CTGCCTACAG CCTCCTGCTG TTCCTGCTCC AGATTAAAGA TCGACATAAT 
GGAAATATCA TGTTGGATAA AAAGGGGCAC ATTATCCACA TTGATTTTGG TTTCATGTTT 
GAAAGCTCCC CAGGGGGCAA CCTGGGATGG GAGCCAGATA TAAAGCTTAC TGATGAGATG 
GTGATGATTA TGGGGGGCAA GATGGAAGCT ACACCTTTCA AATGGTTCAT GGAGATGTGC 
GTGCGTGGCT ACTTAGCTGT AAGGCCGTAC ATGGATGCTG TGGTGTCCCT AGTCACTCTT 
ATGCTGGACA CAGGATTGCC ATGTTTCCGT GGACAAACCA TTAAACTACT GAAGCAGAGG 
TTCAGCCCCA ACTCAACAGA GAGGGAAGCA GCCAATTTCA TCTTAAAGGT CATTCAAAAT 
TGCTTCCTCA GCAACAGGAG CCGAACCTAC GATATGATCC AGTATTATCA GAATGACATC 
CCCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pi4ka ( XM_031905885.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761745.1 Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X2, mRNA.

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CloneID	OXa47457
Clone ID Related Accession (Same CDS sequence)	XM_031905885.1
Accession Version	XM_031905885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphatidylinositol 4-kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA AGGGGGCTTG CAGCGGGTCG CGGGGGTTTT ATTTTAACAC TGTGCTGTCG 
CTGGCCCGTT CCCTGGCAGT GCAGAGGCCT GCTGCATTTG AGAAGGTGCA GAAGCTGCTG 
TGCATGTGCC CTGTCGATTA TCATGGGGTT TTTCAGCTGG ATGAAAGGCG AAGAGATGCA 
CTTATTGCTT TAGGAATATT TCTGGTGGAG TCAGATCTTC AGCACAAAGA GACTGTAGTT 
CCATACCTTT TGAGACTCTT GAGGGGACTT CCAAAGGTGC AGTGGATAGA GGAAAGCAAT 
TCTCGTAAGG GAAAAGGGAC GCTTCCAGTG GCAGAAACCT TCAGCTTCTG TTTAGTTACA 
CTACTGTCGG ATGTTGCGCA CAGGGATCTG TCCTTCAGAG AGCAGATCTT AGAAGCTGTT 
TTGGATGTGA TGCAGTGTCT GCTGGGAATG TGCCAGTCAC CCCAAATTCA GGATAAAGAA 
TACTTGTGTA AATATGCAGT TCCATGCTTG ATTGGAATCT CACGGGCCTT TGGACGATAC 
AGCAACACTG AAGAGTCTCT TTTATCCAAG CTGTTCCCCA AAGTTTCCCC ACAGTCACTA 
AAGGCGCCAG AGGAATTAGA AGGAATACGT AGACGTTCCT TTAATGACTT CCGCTCCATC 
CTGCCAAGCT CACTGCTTAC AGTGTGCCAG GGGGATTCTC TAAAGCGGAA AGCCAGCAGT 
GTTTCAAGTG GCTCACAGGT TAGCCCTGAT CGCATTGTTC CTCCTCCAGG CTCACCTGCC 
GGCTCTACTA TTCCTTACTT TGAAGGGTCA TATTTGCCAG ATGGCAGTGC TGTGGATCCT 
GACTATTACC TTACCACCAT CAGCTCTAGT TTCTCAGTCT CACCTCTCTT CACTGGTGTC 
AACAACAAGG AGTTTGATAT GCCCCAAGAT ACTTTGCGTC AGCTACTCCA TATGGTGAAA 
CAGATTGTGG CAGACCCATT CCTGAGTTCT TTGGATATGC TCATCTCTGA AGTTACAGAA 
GCCAATCCTA GTGTGGACCT ATATTATAAA ACATTCAGTG ATCCCCTCTA CGTGGCAGTG 
TTTAAGATGC TCAGAGATAC ACTTTATAAT GTTAAAGATC CCCATACTTC ATTTGTGAAG 
GAGATTCATG ACTTTGTGCT TGAACAGTTT AACAGCAGCC AGGGTGAGCT GCAGAAAATT 
CTACATGATA TGGATCGACT GAATAATGAA CTCAGCCCCT TGAAATTACG TTGTCAGGCA 
AATGCAGCAT GTGTGGACCT TATGGTGTGG GCTGTCAAAG ATGAACAAGG TGCAGAAAAT 
CTTTGCATCA AACTATCTGA AAAGCTGCAG TCCAAGACAT CTAGCAAAGT GATTATAGCC 
CATATGCCAC TTCTAATATG CTGTTTACAG GGGCTAGGGC GTCTTTGTGA GCGGTTTCCT 
GTAGTTGTCC ATTCTGTGAC GCCTTCGCTT CGAGATTTTC TTGTTATCCC ATCTCCGGTG 
CTGGTGAAGC TTTATAAATA CCATAGCCAA TATCAGACAG GTGCTAATGA TATAAAGATC 
AGTGTAACCA ATGAGCATTC CGAATCCACT ATGAACATTA TGTCTGGCAA GAAAAGCCAG 
GCATCTATGT ATGAACAGCT ACGGGATATT GCCATAGATA ACATCTGCAG GTGTTTAAAA 
GCTGGGTTAA CCATAGATCC TGTCATTGTT GAAGCTTTTC TTGCCAGTTT ATCAAATCGC 
TTGTATATAT CTCAGGAGAA TGAAAAGGAT GCTCATTTGA TACCTGATCA TACAATCCGT 
GCGTTGGGGC ACATTGCTGT TGCTCTAAGG GACACCCCCA AAGTGATGCA ACCCATCCTG 
CAGATCCTTC AGCAGAAGTT CTGCCAGCCC CCTTCACAGC TGGATGTGCT CATTATAGAC 
CAACTAGGGT GCATGGTGAT CACTGGCAAT CAATATATCT ATCAAGAAGT GTGGAACCTT 
TTCCAGCAGA TTAGTGTGAA GGCCAGTTCA GTGGTATACT CTGCCACCAA GGACTATAAA 
GACCATGGCT ACAGACACTG CTCCTTGGCT GTGATTAACG CCCTGGCAAA TATTGCAGCC 
AACATCCAGG GAGAGCACCA GATAGATGAT TTGCTGATGA ATTTGTTGGA GCTTTTTGTG 
CAGCTAGGAC TAGAAGGAAA GAGAGCCAGT GAGAGGGCTA GCGAAAAGGG GCCTGCATTG 
AAGGCCTCCA GCAGTGCAGG AAATCTGGGA GTGCTGATAC CCGTAATTGC TGTGCTCACC 
CGAAGGCTTC CACCAATAAA AGAGGCCAAG CCACGACTTC AGAAACTCTT CAGAGATTTT 
TGGTTGTACT CTGTGCTGAT GGGTTTTGCA GTGGAAGGAT CAGGTCTCTG GCCTGAGGAA 
TGGTATGAAG GTGTCTGTGA AATTGCCACT AAATCTCCTT TGCTGACGTT CCCCAGCAAA 
GAGCCTCTGC GCTCTGTACT GCAGTACAAC TCTGCCATGA AAAATGACAC TGTAACGCCA 
GCAGAACTTA ATGAGCTGAG AAGCACAATT ATCAACCTTC TTGATCCTGC TCCGGAAGTG 
TCTGCACTCA TCAACAAACT GGACTTTGCC ATGTCTACCT ACCTGCTCTC TGTGTACCGC 
CTGGAGCACA TGAGGGTTCT GCATGCCACT GATAAGGATC GATTTCAAGT TATGTTTTTT 
TACTTCGAGG ATAAAGCTAT CCAAAAGGAT AAATCAGGTA TGATGCAGTG TGTTATTGCA 
GTAGCAGACA AAGTGTTTGA AGCTTTTCTG AACGTGATGG CTGATAAGCC TAAAACAAAG 
GAGAACGAGG AAGAATTGGA GCGCCATGCA CAATTCCTGC TGGTAAACTT TAATCACATC 
CACAAGCGCA TTCGACGGGT CGCTGACAAA TACTTGTCAG GGCTAGTGGA CAAATTTCCC 
CATTTGCTGT GGAGTGGTAC TGTGCTGAAA ACCATGCTGG ACATACTGCA GACTCTTTCC 
TTGTCTCTTA GTGCAGACAT TCACAAAGAC CAGCCTTATT ATGACATTCC AGATGCACCT 
TATAGAATAA CCGTTCCCGA TACCTATGAG GCCAGAGAGA GCATTGTCAA AGACTTTGCA 
GCTCGATGTG GCATGATTCT TCAGGAAGCA ATGAAATGGG CTCCTTCAGT GACCAAGTCC 
CACCTGCAGG AATATCTAAA CAAGCACCAG AACTGGATGT CTGGCCTTTC CCAGCACACT 
GGTCTTGCCA TGGCTACTGA AAGCATACTG CATTTTGCTG GGTACAACAG GCAGAATACT 
ACCTTGGGGG TGACACAACT AACAGAAAGG CCAGCCTGTG TCAAGAAGGA TTATTCAAAC 
TTCATGGCAT CTCTCAACCT GCGCAACCGT TATGCTGGAG AGGTTGCAGG CATGATTGAG 
TTTTCTGATG CCACTGGCCG AACTTCTGAC TTAAATAGGC TCATGCTGAA GCACCTGGGC 
GATGCCTTGG ACTGCGGCCA ACCAGAGTCT TATACACAGG TCATGTTCAA ACTTACTGCG 
CTGCTCATTA GCAGCAAAGA GTGTGATGCT CAGCTTCTGC ACCACCTGTG CTGGGCACCC 
CTGCGGATGT TCACTGATCA TGGTATGGAG ACCGCTATTG CCTGCTGGGA CTGGTTGCTT 
GCAGCTAAAA ATGGCATTGA AGTACCGTTT ATGCGTGAAA TGGCTGGTGC TTGGCAAATG 
ACTGTAGAGC AAAAGATTGG ACTTTTCTCA GCAGAAAAGA AGGAGGCAGA TCCTCTTGCT 
GCATCTGAAC AGAGCCAACC ACGTCCATGT GCACCACAAG TGAACCCACA TTATATTTGG 
ATTGATTTTC TCATGCAAAG GTTTGAGATT GCCAAATACT GCAGCTCTGA TCAGGTGGAA 
ATCTTCTCCA GCCTTCTGCA GCGCTCCCTG TCCCTAAACA TTGGTGGTTG TAAAGGAAGT 
CTTAACCGAC ATGTGGCAGC TATTGGCCCT CGATTTAAGT TGCTGACTCT CGGGCTGTCC 
CTGTTACATG CAGACGTGGT CCCAAATGCG ACAATCCGGA ATGTCCTTAG AGAAAAGATC 
TACTCTACAG CATTTGATTA TTTTAGTTGC CCTCCTAAGT TCCCGACTCA GACGCAGAAG 
CTGCTAAGGG AGGATATCAG CATCATGATC AAGTTCTGGA CAGCCATGTT TTCTGATAAA 
AAATATCTCA CTGCCAATCA GCTAGTACCC CCAGATAATC AAGACCCTTC TGTAAACAGC 
TTGTCTGTGA TGTCAGATGT GAACCGCAGC AATTTGGATG TGACTGTTGG CTCCCGACAG 
CAAACAGCTC AGGGCTGGAT CAATACATAC CCTCTGTCCA GTGGAATGTC CACCATCTCT 
AAAAAATCTG GTTTAGCAAA GAAAACAAAC CGTGGGACTC AGCTGCACAA GTACTATATG 
AAGAGGCGGA CTCTGTTGCT GTCACTTTTG GCCACTGAGA TTGAACGCCT GACCACATGG 
TACAACCCCT TGTCCTCCCC TGAGTTGGAA CTGGATCAGA CAGGTGAAAA CAGTGTGGCT 
AACTGGAGAT CTAAGTACAT TAGTTTGAGT GAGAAGCAGT GGAAGGACAA TGTGAACTTA 
GCATGGAGCA TCTCCCCTCA CTTGGCTTTG CAATTACCCA CCAGGTTCAA GAACACTGAG 
GCTATTGGGG GAGAAGTGAC CCGCCTGGTT AGATTGGACC CAGGAGCAGT GAGTGACACT 
CCAGAAGCAA TAAAGTACTT GGTAACATGG CACACAATAG ATGCTGATGC TCCTGAACTT 
AGTCACATAC TCTGCTGGAC ACCGACTGAC CCACCTACAG GACTCTCCTA CTTCTCTAGC 
ATGTACCCAC CACATCCACT TACTGCGCAA TATGGCGTGA AAGTGCTGCG ATCATTCCCA 
CCGGATGCAA TCTTGTTCTA CATTCCGCAG ATAGTTCAAG CCTTGAGATA TGATAAGATG 
GGTTACGTTC GAGAATACAT TCTATGGGCA GCTGCCAAAT CACAGCTTTT GGCTCATCAG 
TTTATTTGGA ACATGAAGAC AAATATTTTC CTTGATGAGG AAGGGCACCA AAAAGACCCT 
GATATTGGAG AACTATTGGA GCAGCTGGTT GAAGAAATTA CAGGATCTCT GTCTGGGCCA 
GCCAAGGATT TCTACCAGCG AGAATTTGAC TTTTTCAATA AGATCACAAA TGTCTCTGCC 
ATCATTAAGC CATTTCCAAA AGGAGAAGAA CGGAAAAAGG CCTGTCTGAA CTCTCTTTCA 
GAAGTAAAAG TGCAGCCAGG TTGTTACCTG CCCAGTAATC CAGAAGCCAT TGTGTTGGAC 
ATTGATTACA CATCTGGGAC CCCTTTGCAG AGTGCTGCGA AGGCTCCTTA TTTGGCCAAA 
TTTAAGGTGA AGCGATGTGG AGTAAGTGAA CTGGAGAAGG AAGGTCTCAG CTGCCGTTCA 
GATTCTATTG ATGAGAGTGA GGAGGAAGGT GGAGATGGGA AGGTTTGCTG GCAAGCGGCT 
ATCTTTAAGG TCGGAGATGA CTGCAGACAG GATATGCTGG CTCTGCAGAT CATTGGTCTC 
TTTAAGAACA TTTTCCAGCT GGTGGGACTT GATCTCTTTG TTTTCCCATA CAGAGTGGTA 
GCAACAGCTC CAGGGTGCGG AGTTATTGAA TGTATACCAG ACTGCACCTC GCGGGACCAG 
CTCGGTCGCC AAACAGATTT TGGAATGTAT GACTATTTTA CTCGGCAGTA TGGGGATGAA 
TCAGCTCTTG CTTTCCAAAA GGCTCGATAT AACTTTATCC GAAGTATGGC TGCCTACAGC 
CTCCTGCTGT TCCTGCTCCA GATTAAAGAT CGACATAATG GAAATATCAT GTTGGATAAA 
AAGGGGCACA TTATCCACAT TGATTTTGGT TTCATGTTTG AAAGCTCCCC AGGGGGCAAC 
CTGGGATGGG AGCCAGATAT AAAGCTTACT GATGAGATGG TGATGATTAT GGGGGGCAAG 
ATGGAAGCTA CACCTTTCAA ATGGTTCATG GAGATGTGCG TGCGTGGCTA CTTAGCTGTA 
AGGCCGTACA TGGATGCTGT GGTGTCCCTA GTCACTCTTA TGCTGGACAC AGGATTGCCA 
TGTTTCCGTG GACAAACCAT TAAACTACTG AAGCAGAGGT TCAGCCCCAA CTCAACAGAG 
AGGGAAGCAG CCAATTTCAT CTTAAAGGTC ATTCAAAATT GCTTCCTCAG CAACAGGAGC 
CGAACCTACG ATATGATCCA GTATTATCAG AATGACATCC CCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761745.1
CDS	21..6308
Translation	MAAKGACSGSRGFYFNTVLSLARSLAVQRPAAFEKVQKLLCMCPVDYHGVFQLDERRRDALIALGIFLVESDLQHKETVVPYLLRLLRGLPKVQWIEESNSRKGKGTLPVAETFSFCLVTLLSDVAHRDLSFREQILEAVLDVMQCLLGMCQSPQIQDKEYLCKYAVPCLIGISRAFGRYSNTEESLLSKLFPKVSPQSLKAPEELEGIRRRSFNDFRSILPSSLLTVCQGDSLKRKASSVSSGSQVSPDRIVPPPGSPAGSTIPYFEGSYLPDGSAVDPDYYLTTISSSFSVSPLFTGVNNKEFDMPQDTLRQLLHMVKQIVADPFLSSLDMLISEVTEANPSVDLYYKTFSDPLYVAVFKMLRDTLYNVKDPHTSFVKEIHDFVLEQFNSSQGELQKILHDMDRLNNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHMPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYQTGANDIKISVTNEHSESTMNIMSGKKSQASMYEQLRDIAIDNICRCLKAGLTIDPVIVEAFLASLSNRLYISQENEKDAHLIPDHTIRALGHIAVALRDTPKVMQPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQGEHQIDDLLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELNELRSTIINLLDPAPEVSALINKLDFAMSTYLLSVYRLEHMRVLHATDKDRFQVMFFYFEDKAIQKDKSGMMQCVIAVADKVFEAFLNVMADKPKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPSVTKSHLQEYLNKHQNWMSGLSQHTGLAMATESILHFAGYNRQNTTLGVTQLTERPACVKKDYSNFMASLNLRNRYAGEVAGMIEFSDATGRTSDLNRLMLKHLGDALDCGQPESYTQVMFKLTALLISSKECDAQLLHHLCWAPLRMFTDHGMETAIACWDWLLAAKNGIEVPFMREMAGAWQMTVEQKIGLFSAEKKEADPLAASEQSQPRPCAPQVNPHYIWIDFLMQRFEIAKYCSSDQVEIFSSLLQRSLSLNIGGCKGSLNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQTQKLLREDISIMIKFWTAMFSDKKYLTANQLVPPDNQDPSVNSLSVMSDVNRSNLDVTVGSRQQTAQGWINTYPLSSGMSTISKKSGLAKKTNRGTQLHKYYMKRRTLLLSLLATEIERLTTWYNPLSSPELELDQTGENSVANWRSKYISLSEKQWKDNVNLAWSISPHLALQLPTRFKNTEAIGGEVTRLVRLDPGAVSDTPEAIKYLVTWHTIDADAPELSHILCWTPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPFPKGEERKKACLNSLSEVKVQPGCYLPSNPEAIVLDIDYTSGTPLQSAAKAPYLAKFKVKRCGVSELEKEGLSCRSDSIDESEEEGGDGKVCWQAAIFKVGDDCRQDMLALQIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESALAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKQRFSPNSTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	XM_031905885.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905885.1

ORF Insert Sequence:

ATGGCGGCCA AGGGGGCTTG CAGCGGGTCG CGGGGGTTTT ATTTTAACAC TGTGCTGTCG 
CTGGCCCGTT CCCTGGCAGT GCAGAGGCCT GCTGCATTTG AGAAGGTGCA GAAGCTGCTG 
TGCATGTGCC CTGTCGATTA TCATGGGGTT TTTCAGCTGG ATGAAAGGCG AAGAGATGCA 
CTTATTGCTT TAGGAATATT TCTGGTGGAG TCAGATCTTC AGCACAAAGA GACTGTAGTT 
CCATACCTTT TGAGACTCTT GAGGGGACTT CCAAAGGTGC AGTGGATAGA GGAAAGCAAT 
TCTCGTAAGG GAAAAGGGAC GCTTCCAGTG GCAGAAACCT TCAGCTTCTG TTTAGTTACA 
CTACTGTCGG ATGTTGCGCA CAGGGATCTG TCCTTCAGAG AGCAGATCTT AGAAGCTGTT 
TTGGATGTGA TGCAGTGTCT GCTGGGAATG TGCCAGTCAC CCCAAATTCA GGATAAAGAA 
TACTTGTGTA AATATGCAGT TCCATGCTTG ATTGGAATCT CACGGGCCTT TGGACGATAC 
AGCAACACTG AAGAGTCTCT TTTATCCAAG CTGTTCCCCA AAGTTTCCCC ACAGTCACTA 
AAGGCGCCAG AGGAATTAGA AGGAATACGT AGACGTTCCT TTAATGACTT CCGCTCCATC 
CTGCCAAGCT CACTGCTTAC AGTGTGCCAG GGGGATTCTC TAAAGCGGAA AGCCAGCAGT 
GTTTCAAGTG GCTCACAGGT TAGCCCTGAT CGCATTGTTC CTCCTCCAGG CTCACCTGCC 
GGCTCTACTA TTCCTTACTT TGAAGGGTCA TATTTGCCAG ATGGCAGTGC TGTGGATCCT 
GACTATTACC TTACCACCAT CAGCTCTAGT TTCTCAGTCT CACCTCTCTT CACTGGTGTC 
AACAACAAGG AGTTTGATAT GCCCCAAGAT ACTTTGCGTC AGCTACTCCA TATGGTGAAA 
CAGATTGTGG CAGACCCATT CCTGAGTTCT TTGGATATGC TCATCTCTGA AGTTACAGAA 
GCCAATCCTA GTGTGGACCT ATATTATAAA ACATTCAGTG ATCCCCTCTA CGTGGCAGTG 
TTTAAGATGC TCAGAGATAC ACTTTATAAT GTTAAAGATC CCCATACTTC ATTTGTGAAG 
GAGATTCATG ACTTTGTGCT TGAACAGTTT AACAGCAGCC AGGGTGAGCT GCAGAAAATT 
CTACATGATA TGGATCGACT GAATAATGAA CTCAGCCCCT TGAAATTACG TTGTCAGGCA 
AATGCAGCAT GTGTGGACCT TATGGTGTGG GCTGTCAAAG ATGAACAAGG TGCAGAAAAT 
CTTTGCATCA AACTATCTGA AAAGCTGCAG TCCAAGACAT CTAGCAAAGT GATTATAGCC 
CATATGCCAC TTCTAATATG CTGTTTACAG GGGCTAGGGC GTCTTTGTGA GCGGTTTCCT 
GTAGTTGTCC ATTCTGTGAC GCCTTCGCTT CGAGATTTTC TTGTTATCCC ATCTCCGGTG 
CTGGTGAAGC TTTATAAATA CCATAGCCAA TATCAGACAG GTGCTAATGA TATAAAGATC 
AGTGTAACCA ATGAGCATTC CGAATCCACT ATGAACATTA TGTCTGGCAA GAAAAGCCAG 
GCATCTATGT ATGAACAGCT ACGGGATATT GCCATAGATA ACATCTGCAG GTGTTTAAAA 
GCTGGGTTAA CCATAGATCC TGTCATTGTT GAAGCTTTTC TTGCCAGTTT ATCAAATCGC 
TTGTATATAT CTCAGGAGAA TGAAAAGGAT GCTCATTTGA TACCTGATCA TACAATCCGT 
GCGTTGGGGC ACATTGCTGT TGCTCTAAGG GACACCCCCA AAGTGATGCA ACCCATCCTG 
CAGATCCTTC AGCAGAAGTT CTGCCAGCCC CCTTCACAGC TGGATGTGCT CATTATAGAC 
CAACTAGGGT GCATGGTGAT CACTGGCAAT CAATATATCT ATCAAGAAGT GTGGAACCTT 
TTCCAGCAGA TTAGTGTGAA GGCCAGTTCA GTGGTATACT CTGCCACCAA GGACTATAAA 
GACCATGGCT ACAGACACTG CTCCTTGGCT GTGATTAACG CCCTGGCAAA TATTGCAGCC 
AACATCCAGG GAGAGCACCA GATAGATGAT TTGCTGATGA ATTTGTTGGA GCTTTTTGTG 
CAGCTAGGAC TAGAAGGAAA GAGAGCCAGT GAGAGGGCTA GCGAAAAGGG GCCTGCATTG 
AAGGCCTCCA GCAGTGCAGG AAATCTGGGA GTGCTGATAC CCGTAATTGC TGTGCTCACC 
CGAAGGCTTC CACCAATAAA AGAGGCCAAG CCACGACTTC AGAAACTCTT CAGAGATTTT 
TGGTTGTACT CTGTGCTGAT GGGTTTTGCA GTGGAAGGAT CAGGTCTCTG GCCTGAGGAA 
TGGTATGAAG GTGTCTGTGA AATTGCCACT AAATCTCCTT TGCTGACGTT CCCCAGCAAA 
GAGCCTCTGC GCTCTGTACT GCAGTACAAC TCTGCCATGA AAAATGACAC TGTAACGCCA 
GCAGAACTTA ATGAGCTGAG AAGCACAATT ATCAACCTTC TTGATCCTGC TCCGGAAGTG 
TCTGCACTCA TCAACAAACT GGACTTTGCC ATGTCTACCT ACCTGCTCTC TGTGTACCGC 
CTGGAGCACA TGAGGGTTCT GCATGCCACT GATAAGGATC GATTTCAAGT TATGTTTTTT 
TACTTCGAGG ATAAAGCTAT CCAAAAGGAT AAATCAGGTA TGATGCAGTG TGTTATTGCA 
GTAGCAGACA AAGTGTTTGA AGCTTTTCTG AACGTGATGG CTGATAAGCC TAAAACAAAG 
GAGAACGAGG AAGAATTGGA GCGCCATGCA CAATTCCTGC TGGTAAACTT TAATCACATC 
CACAAGCGCA TTCGACGGGT CGCTGACAAA TACTTGTCAG GGCTAGTGGA CAAATTTCCC 
CATTTGCTGT GGAGTGGTAC TGTGCTGAAA ACCATGCTGG ACATACTGCA GACTCTTTCC 
TTGTCTCTTA GTGCAGACAT TCACAAAGAC CAGCCTTATT ATGACATTCC AGATGCACCT 
TATAGAATAA CCGTTCCCGA TACCTATGAG GCCAGAGAGA GCATTGTCAA AGACTTTGCA 
GCTCGATGTG GCATGATTCT TCAGGAAGCA ATGAAATGGG CTCCTTCAGT GACCAAGTCC 
CACCTGCAGG AATATCTAAA CAAGCACCAG AACTGGATGT CTGGCCTTTC CCAGCACACT 
GGTCTTGCCA TGGCTACTGA AAGCATACTG CATTTTGCTG GGTACAACAG GCAGAATACT 
ACCTTGGGGG TGACACAACT AACAGAAAGG CCAGCCTGTG TCAAGAAGGA TTATTCAAAC 
TTCATGGCAT CTCTCAACCT GCGCAACCGT TATGCTGGAG AGGTTGCAGG CATGATTGAG 
TTTTCTGATG CCACTGGCCG AACTTCTGAC TTAAATAGGC TCATGCTGAA GCACCTGGGC 
GATGCCTTGG ACTGCGGCCA ACCAGAGTCT TATACACAGG TCATGTTCAA ACTTACTGCG 
CTGCTCATTA GCAGCAAAGA GTGTGATGCT CAGCTTCTGC ACCACCTGTG CTGGGCACCC 
CTGCGGATGT TCACTGATCA TGGTATGGAG ACCGCTATTG CCTGCTGGGA CTGGTTGCTT 
GCAGCTAAAA ATGGCATTGA AGTACCGTTT ATGCGTGAAA TGGCTGGTGC TTGGCAAATG 
ACTGTAGAGC AAAAGATTGG ACTTTTCTCA GCAGAAAAGA AGGAGGCAGA TCCTCTTGCT 
GCATCTGAAC AGAGCCAACC ACGTCCATGT GCACCACAAG TGAACCCACA TTATATTTGG 
ATTGATTTTC TCATGCAAAG GTTTGAGATT GCCAAATACT GCAGCTCTGA TCAGGTGGAA 
ATCTTCTCCA GCCTTCTGCA GCGCTCCCTG TCCCTAAACA TTGGTGGTTG TAAAGGAAGT 
CTTAACCGAC ATGTGGCAGC TATTGGCCCT CGATTTAAGT TGCTGACTCT CGGGCTGTCC 
CTGTTACATG CAGACGTGGT CCCAAATGCG ACAATCCGGA ATGTCCTTAG AGAAAAGATC 
TACTCTACAG CATTTGATTA TTTTAGTTGC CCTCCTAAGT TCCCGACTCA GACGCAGAAG 
CTGCTAAGGG AGGATATCAG CATCATGATC AAGTTCTGGA CAGCCATGTT TTCTGATAAA 
AAATATCTCA CTGCCAATCA GCTAGTACCC CCAGATAATC AAGACCCTTC TGTAAACAGC 
TTGTCTGTGA TGTCAGATGT GAACCGCAGC AATTTGGATG TGACTGTTGG CTCCCGACAG 
CAAACAGCTC AGGGCTGGAT CAATACATAC CCTCTGTCCA GTGGAATGTC CACCATCTCT 
AAAAAATCTG GTTTAGCAAA GAAAACAAAC CGTGGGACTC AGCTGCACAA GTACTATATG 
AAGAGGCGGA CTCTGTTGCT GTCACTTTTG GCCACTGAGA TTGAACGCCT GACCACATGG 
TACAACCCCT TGTCCTCCCC TGAGTTGGAA CTGGATCAGA CAGGTGAAAA CAGTGTGGCT 
AACTGGAGAT CTAAGTACAT TAGTTTGAGT GAGAAGCAGT GGAAGGACAA TGTGAACTTA 
GCATGGAGCA TCTCCCCTCA CTTGGCTTTG CAATTACCCA CCAGGTTCAA GAACACTGAG 
GCTATTGGGG GAGAAGTGAC CCGCCTGGTT AGATTGGACC CAGGAGCAGT GAGTGACACT 
CCAGAAGCAA TAAAGTACTT GGTAACATGG CACACAATAG ATGCTGATGC TCCTGAACTT 
AGTCACATAC TCTGCTGGAC ACCGACTGAC CCACCTACAG GACTCTCCTA CTTCTCTAGC 
ATGTACCCAC CACATCCACT TACTGCGCAA TATGGCGTGA AAGTGCTGCG ATCATTCCCA 
CCGGATGCAA TCTTGTTCTA CATTCCGCAG ATAGTTCAAG CCTTGAGATA TGATAAGATG 
GGTTACGTTC GAGAATACAT TCTATGGGCA GCTGCCAAAT CACAGCTTTT GGCTCATCAG 
TTTATTTGGA ACATGAAGAC AAATATTTTC CTTGATGAGG AAGGGCACCA AAAAGACCCT 
GATATTGGAG AACTATTGGA GCAGCTGGTT GAAGAAATTA CAGGATCTCT GTCTGGGCCA 
GCCAAGGATT TCTACCAGCG AGAATTTGAC TTTTTCAATA AGATCACAAA TGTCTCTGCC 
ATCATTAAGC CATTTCCAAA AGGAGAAGAA CGGAAAAAGG CCTGTCTGAA CTCTCTTTCA 
GAAGTAAAAG TGCAGCCAGG TTGTTACCTG CCCAGTAATC CAGAAGCCAT TGTGTTGGAC 
ATTGATTACA CATCTGGGAC CCCTTTGCAG AGTGCTGCGA AGGCTCCTTA TTTGGCCAAA 
TTTAAGGTGA AGCGATGTGG AGTAAGTGAA CTGGAGAAGG AAGGTCTCAG CTGCCGTTCA 
GATTCTATTG ATGAGAGTGA GGAGGAAGGT GGAGATGGGA AGGTTTGCTG GCAAGCGGCT 
ATCTTTAAGG TCGGAGATGA CTGCAGACAG GATATGCTGG CTCTGCAGAT CATTGGTCTC 
TTTAAGAACA TTTTCCAGCT GGTGGGACTT GATCTCTTTG TTTTCCCATA CAGAGTGGTA 
GCAACAGCTC CAGGGTGCGG AGTTATTGAA TGTATACCAG ACTGCACCTC GCGGGACCAG 
CTCGGTCGCC AAACAGATTT TGGAATGTAT GACTATTTTA CTCGGCAGTA TGGGGATGAA 
TCAGCTCTTG CTTTCCAAAA GGCTCGATAT AACTTTATCC GAAGTATGGC TGCCTACAGC 
CTCCTGCTGT TCCTGCTCCA GATTAAAGAT CGACATAATG GAAATATCAT GTTGGATAAA 
AAGGGGCACA TTATCCACAT TGATTTTGGT TTCATGTTTG AAAGCTCCCC AGGGGGCAAC 
CTGGGATGGG AGCCAGATAT AAAGCTTACT GATGAGATGG TGATGATTAT GGGGGGCAAG 
ATGGAAGCTA CACCTTTCAA ATGGTTCATG GAGATGTGCG TGCGTGGCTA CTTAGCTGTA 
AGGCCGTACA TGGATGCTGT GGTGTCCCTA GTCACTCTTA TGCTGGACAC AGGATTGCCA 
TGTTTCCGTG GACAAACCAT TAAACTACTG AAGCAGAGGT TCAGCCCCAA CTCAACAGAG 
AGGGAAGCAG CCAATTTCAT CTTAAAGGTC ATTCAAAATT GCTTCCTCAG CAACAGGAGC 
CGAACCTACG ATATGATCCA GTATTATCAG AATGACATCC CCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pi4ka ( NM_001251875.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001238804.1 Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), mRNA.

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CloneID	OXa20413
Clone ID Related Accession (Same CDS sequence)	NM_001251875.2
Accession Version	NM_001251875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphatidylinositol 4-kinase alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03003899.1, CX809688.1, CX889604.1, CX825096.1, DT400274.1, CX971967.1 and BC154726.1. On Aug 9, 2013 this sequence version replaced NM_001251875.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2431823, SAMEA2431828 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCCCTG TCGATTATCA TGGGGTTTTT CAGCTGGATG AAAGGCGAAG AGATGCACTT 
ATTGCTTTAG GAATATTTCT GGTGGAGTCA GATCTTCAGC ACAAAGAGAC TGTAGTTCCA 
TACCTTTTGA GACTCTTGAG GGGACTTCCA AAGGTGCAGT GGATAGAGGA AAGCAATTCT 
CGTAAGGGAA AAGGGACACT TCCAGTGGCA GAAACCTTCA GCTTCTGTTT AGTTACACTA 
CTGTCGGATG TTGCGCACAG GGATCTGTCC TTCAGAGAGC AGATCTTAGA AGCTGTTTTG 
GATGTGATGC AGTGTCTGCT GGGAATGTGC CAGTCACCCC AAATTCAGGA TAAAGAATAC 
TTGTGTAAAT ATGCAGTTCC ATGCTTGATT GGAATCTCAC GGGCCTTTGG ACGATACAGC 
AACACTGAAG AGTCTCTTTT ATCCAAGCTG TTCCCCAAAG TTTCCCCACA GTCACTAAAG 
GCGCCAGAGG AATTAGAAGG AATACGTAGA CGTTCCTTTA ATGACTTCCG CTCCATCCTG 
CCAAGCTCAC TGCTTACAGT GTGCCAGGGG GATTCTCTAA AGCGGAAAGC CAGCAGTGTT 
TCAAGTGGCT CACAGGTTAG CCCTGATCGC ATTGTTCCTC CTCCAGGCTC ACCTGCCGGC 
TCTACTATTC CTTACTTTGA AGGGTCATAT TTGCCAGATG GCAGTGCTGT GGATCCTGAC 
TATTACCTTA CCACCATCAG CTCTAGTTTC TCAGTCTCAC CTCTCTTCAC TGGTGTCAAC 
AACAAGGAGT TTGATATGCC CCAAGATACT TTGCGTCAGC TACTCCATAT GGTGAAACAG 
ATTGTGGCAG ACCCATTCCT GAGTTCTTTG GATATGCTCA TCTCTGAAGT TACAGAAGCC 
AATCCTAGTG TGGACCTATA TTATAAAACA TTCAGTGATC CCCTCTACGT GGCAGTGTTT 
AAGATGCTCA GAGATACACT TTATAATGTT AAAGATCCCC ATACTTCATT TGTGAAGGAG 
ATTCATGACT TTGTGCTTGA ACAGTTTAAC AGCAGCCAGG GTGAGCTGCA GAAAATTCTA 
CATGATATGG ATCGACTGAA TAATGAACTC AGCCCCTTGA AATTACGTTG TCAGGCAAAT 
GCAGCATGTG TGGACCTTAT GGTGTGGGCT GTCAAAGATG AACAAGGTGC AGAAAATCTT 
TGCATCAAAC TATCTGAAAA GCTGCAGTCC AAGACATCTA GCAAAGTGAT TATAGCCCAT 
ATGCCACTTC TAATATGCTG TTTACAGGGG CTAGGGCGTC TTTGTGAGCG GTTTCCTGTA 
GTTGTCCATT CTGTGACGCC TTCGCTTCGA GATTTTCTTG TGATCCCATC TCCGGTGCTG 
GTGAAGCTTT ATAAATACCA TAGCCAATAT CAGACAGGTG CTAATGATAT AAAGATCAGT 
GTAACCAATG AGCATTCCGA ATCCACTATG AACATTATGT CTGGCAAGAA AAGCCAGGCA 
TCTATGTATG AACAGCTGCG GGATATTGCC ATAGATAACA TCTGCAGGTG TTTAAAAGCT 
GGGTTAACCA TAGATCCTGT CATTGTTGAA GCTTTTCTTG CCAGTTTATC AAATCGCTTG 
TATATATCTC AGGAGAATGA AAAGGATGCT CATTTGATAC CTGATCATAC AATCCGTGCG 
TTGGGGCACA TTGCTGTTGC TCTAAGGGAC ACCCCCAAAG TGATGCAACC CATCCTGCAG 
ATCCTTCAGC AGAAGTTCTG CCAGCCCCCT TCACAGCTGG ATGTGCTCAT TATAGACCAA 
CTAGGGTGCA TGGTGATCAC TGGCAATCAA TATATCTATC AAGAAGTGTG GAACCTTTTC 
CAACAGATTA GTGTGAAGGC CAGTTCAGTG GTATACTCTG CCACCAAGGA CTATAAAGAC 
CATGGCTACA GACACTGCTC CTTGGCTGTG ATTAACGCCC TGGCAAATAT TGCAGCCAAC 
ATCCAGGGAG AGCACCAGAT AGATGATTTG CTGATGAATT TGTTGGAGCT TTTTGTGCAG 
CTAGGACTAG AAGGAAAGAG AGCCAGTGAG AGGGCTAGCG AAAAGGGGCC TGCATTGAAG 
GCCTCCAGCA GTGCAGGAAA TCTGGGAGTG CTGATACCCG TAATTGCTGT GCTCACCCGA 
AGGCTTCCAC CAATAAAAGA GGCCAAGCCA CGACTTCAGA AACTCTTCAG AGATTTTTGG 
TTGTACTCTG TGCTGATGGG TTTTGCAGTG GAAGGATCAG GTCTCTGGCC TGAGGAATGG 
TATGAAGGTG TCTGTGAAAT TGCCACTAAA TCTCCTTTGC TGACGTTCCC CAGCAAAGAG 
CCTCTGCGCT CTGTACTGCA GTACAACTCT GCCATGAAAA ATGACACTGT AACGCCAGCA 
GAACTTAATG AGCTGAGAAG CACAATTATC AACCTTCTTG ATCCTGCTCC GGAAGTGTCT 
GCACTCATCA ACAAACTGGA CTTTGCCATG TCTACCTACC TGCTCTCTGT GTACCGCCTG 
GAGCACATGA GGGTTCTGCA TGCCACTGAT AAGGATCGAT TTCAAGTTAT GTTTTTTTAC 
TTCGAGGATA AAGCTATCCA AAAGGATAAA TCAGGTATGA TGCAGTGTGT TATTGCAGTA 
GCAGACAAAG TGTTTGAAGC TTTTCTGAAC GTGATGGCTG ATAAGCCTAA AACGAAAGAG 
AACGAGGAAG AATTGGAGCG CCATGCACAA TTCCTGCTGG TAAACTTTAA TCACATCCAC 
AAGCGCATTC GACGGGTCGC TGACAAATAC TTGTCAGGGC TAGTGGACAA ATTTCCCCAT 
TTGCTGTGGA GTGGTACTGT GCTGAAAACC ATGCTGGACA TACTGCAGAC TCTTTCCTTG 
TCTCTTAGTG CAGACATTCA CAAAGACCAG CCTTATTATG ACATTCCAGA TGCACCTTAT 
AGAATAACCG TTCCCGATAC CTATGAGGCC AGAGAGAGCA TTGTCAAAGA CTTTGCAGCT 
CGATGTGGCA TGATTCTTCA GGAAGCAATG AAATGGGCTC CTTCAGTGAC CAAGTCCCAC 
CTGCAGGAAT ATCTAAACAA GCACCAGAAC TGGATGTCTG GCCTTTCCCA GCACACTGGT 
CTTGCCATGG CTACTGAAAG CATACTGCAT TTTGCTGGGT ACAACAGGCA GAATACTACC 
TTGGGGTCTC CAGCTTCCTC TGCCCAGGTG ACACAACTAA CAGAAAGGCC AGCCTGTGTC 
AAGAAGGATT ATTCAAACTT CATGGCATCT CTCAACCTGC GCAACCGTTA TGCTGGAGAG 
GTTGCAGGCA TGATTGAGTT TTCTGATGCC ACTGGCCGAA CTTCTGACTT AAATAGGCTC 
ATGCTGAAGC ACCTGGGCGA TGCCTTGGAC TGCGGCCAAC CAGAGTCTTA TACACAGGTC 
ATGTTCAAAC TTACTGCGCT GCTCATTAGC AGCAAAGAGT GTGATGCTCA GCTTCTGCAC 
CACCTGTGCT GGGCACCCCT GCGGATGTTC ACTGATCATG GTATGGAGAC CGCTATTGCC 
TGCTGGGACT GGTTGCTTGC AGCTAAAAAT GGCATTGAAG TACCGTTTAT GCGTGAAATG 
GCTGGTGCTT GGCAAATGAC TGTAGAGCAA AAGATTGGAC TTTTCTCAGC AGAAAAGAAG 
GAGGCAGATC CTCTTGCTGC ATCTGAACAG AGCCAACCAC GTCCATGTGC ACCACAAGTG 
AACCCACATT ATATTTGGAT TGATTTTCTC ATGCAAAGGT TTGAGATTGC CAAATACTGC 
AGCTCTGATC AGGTGGAAAT CTTCTCCAGC CTTCTGCAGC GCTCCCTGTC CCTAAACATT 
GGTGGTTGTA AAGGAAGTCT TAACCGACAT GTGGCAGCTA TTGGCCCTCG ATTTAAGTTG 
CTGACTCTCG GGCTGTCCCT GTTACATGCA GACGTGGTCC CAAATGCGAC AATCCGGAAT 
GTCCTTAGAG AAAAGATCTA CTCTACAGCA TTTGATTATT TTAGTTGCCC TCCTAAGTTC 
CCGACTCAGA CGCAGAAGCT GCTAAGGGAG GATATCAGCA TCATGATCAA GTTCTGGACA 
GCCATGTTTT CTGATAAAAA ATATCTCACT GCCAATCAGC TAGTACCCCC AGATAATCAA 
GACCCTTCTG TAAACAGCTT GTCTGTGATG TCAGATGTGA ACCGCAGCAA TTTGGATGTG 
ACTGTTGGCT CCCGACAGCA AACAGCTCAG GGCTGGATCA ATACATACCC TCTGTCCAGT 
GGAATGTCCA CCATCTCTAA AAAATCTGGT TTAGCAAAGA AAACAAACCG TGGGACTCAG 
CTGCACAAGT ACTATATGAA GAGGCGGACT CTGTTGCTGT CACTTTTGGC CACTGAGATT 
GAACGCCTGA CCACATGGTA CAACCCCTTG TCCTCCCCTG AGTTGGAACT GGATCAGACA 
GGTGAAAACA GTGTGGCTAA CTGGAGATCT AAGTACATTA GTTTGAGTGA GAAGCAGTGG 
AAGGACAATG TGAACTTAGC ATGGAGCATC TCCCCTCACT TGGCTTTGCA ATTACCCACC 
AGGTTTAAGA ACACTGAGGC TATTGGGGGA GAAGTGACCC GCCTGGTTAG ATTGGACCCA 
GGAGCAGTGA GTGACACTCC AGAAGCAATA AAGTACTTGG TAACATGGCA CACAATAGAT 
GCTGATGCTC CTGAACTTAG TCACATACTC TGCTGGACAC CGACTGACCC ACCTACAGGA 
CTCTCCTACT TCTCTAGCAT GTACCCACCA CATCCACTTA CTGCGCAATA TGGCGTGAAA 
GTGCTGCGAT CATTCCCACC GGATGCAATC TTGTTCTACA TTCCGCAGAT AGTTCAAGCC 
TTGAGATATG ATAAGATGGG TTACGTTCGA GAATACATTC TATGGGCAGC TGCCAAATCA 
CAGCTTTTGG CTCATCAGTT TATTTGGAAC ATGAAGACAA ATATTTTCCT TGATGAGGAA 
GGGCACCAAA AAGACCCTGA TATTGGAGAA CTATTGGAGC AGCTGGTTGA AGAAATTACA 
GGATCTCTGT CTGGGCCAGC CAAGGATTTC TACCAGCGAG AATTTGACTT TTTCAATAAG 
ATCACAAATG TCTCTGCCAT CATTAAGCCA TTTCCAAAAG GAGAAGAACG GAAAAAGGCC 
TGTCTGAACT CTCTTTCAGA AGTAAAAGTG CAGCCAGGTT GTTACCTGCC CAGTAATCCA 
GAAGCCATTG TGTTGGACAT TGATTACACA TCTGGGACCC CTTTGCAGAG TGCTGCGAAG 
GCTCCTTATT TGGCCAAATT TAAGGTGAAG CGATGTGGAG TAAGTGAACT GGAGAAGGAA 
GGTCTCAGCT GCCGTTCAGA TTCTATTGAT GAGAGTGAGG AGGAAGGTGG AGATGGGAAG 
GTTTGCTGGC AAGCGGCTAT CTTTAAGGTC GGAGATGACT GCAGACAGGA TATGCTGGCT 
CTGCAGATCA TTGGTCTCTT TAAGAACATT TTCCAGCTGG TGGGACTTGA TCTCTTTGTT 
TTCCCATACA GAGTGGTAGC AACAGCTCCA GGGTGCGGAG TTATTGAATG TATACCAGAC 
TGCACCTCGC GGGACCAGCT CGGTCGCCAA ACAGATTTTG GAATGTATGA CTATTTTACT 
CGGCAGTATG GGGATGAATC AGCTCTTGCT TTCCAAAAGG CTCGATATAA CTTTATCCGA 
AGTATGGCTG CCTACAGCCT CCTGCTGTTC CTGCTCCAGA TTAAAGATCG ACATAATGGA 
AATATCATGT TGGATAAAAA GGGGCACATT ATCCACATTG ATTTTGGTTT CATGTTTGAA 
AGCTCCCCAG GGGGCAACCT GGGATGGGAG CCAGATATAA AGCTTACTGA TGAGATGGTG 
ATGATTATGG GGGGCAAGAT GGAAGCTACA CCTTTCAAAT GGTTCATGGA GATGTGCGTG 
CGTGGCTACT TAGCTGTAAG GCCGTACATG GATGCTGTGG TGTCCCTAGT CACTCTTATG 
CTGGACACAG GATTGCCATG TTTCCGTGGA CAAACCATTA AACTACTGAA GCAGAGGTTC 
AGCCCCAACT CAACAGAGAG GGAAGCAGCC AATTTCATCT TAAAGGTCAT TCAAAATTGC 
TTCCTCAGCA ACAGGAGCCG AACCTACGAT ATGATCCAGT ATTATCAGAA TGACATCCCC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238804.1
CDS	1..6186
Translation	MCPVDYHGVFQLDERRRDALIALGIFLVESDLQHKETVVPYLLRLLRGLPKVQWIEESNSRKGKGTLPVAETFSFCLVTLLSDVAHRDLSFREQILEAVLDVMQCLLGMCQSPQIQDKEYLCKYAVPCLIGISRAFGRYSNTEESLLSKLFPKVSPQSLKAPEELEGIRRRSFNDFRSILPSSLLTVCQGDSLKRKASSVSSGSQVSPDRIVPPPGSPAGSTIPYFEGSYLPDGSAVDPDYYLTTISSSFSVSPLFTGVNNKEFDMPQDTLRQLLHMVKQIVADPFLSSLDMLISEVTEANPSVDLYYKTFSDPLYVAVFKMLRDTLYNVKDPHTSFVKEIHDFVLEQFNSSQGELQKILHDMDRLNNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHMPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYQTGANDIKISVTNEHSESTMNIMSGKKSQASMYEQLRDIAIDNICRCLKAGLTIDPVIVEAFLASLSNRLYISQENEKDAHLIPDHTIRALGHIAVALRDTPKVMQPILQILQQKFCQPPSQLDVLIIDQLGCMVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQGEHQIDDLLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELNELRSTIINLLDPAPEVSALINKLDFAMSTYLLSVYRLEHMRVLHATDKDRFQVMFFYFEDKAIQKDKSGMMQCVIAVADKVFEAFLNVMADKPKTKENEEELERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPSVTKSHLQEYLNKHQNWMSGLSQHTGLAMATESILHFAGYNRQNTTLGSPASSAQVTQLTERPACVKKDYSNFMASLNLRNRYAGEVAGMIEFSDATGRTSDLNRLMLKHLGDALDCGQPESYTQVMFKLTALLISSKECDAQLLHHLCWAPLRMFTDHGMETAIACWDWLLAAKNGIEVPFMREMAGAWQMTVEQKIGLFSAEKKEADPLAASEQSQPRPCAPQVNPHYIWIDFLMQRFEIAKYCSSDQVEIFSSLLQRSLSLNIGGCKGSLNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQTQKLLREDISIMIKFWTAMFSDKKYLTANQLVPPDNQDPSVNSLSVMSDVNRSNLDVTVGSRQQTAQGWINTYPLSSGMSTISKKSGLAKKTNRGTQLHKYYMKRRTLLLSLLATEIERLTTWYNPLSSPELELDQTGENSVANWRSKYISLSEKQWKDNVNLAWSISPHLALQLPTRFKNTEAIGGEVTRLVRLDPGAVSDTPEAIKYLVTWHTIDADAPELSHILCWTPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPFPKGEERKKACLNSLSEVKVQPGCYLPSNPEAIVLDIDYTSGTPLQSAAKAPYLAKFKVKRCGVSELEKEGLSCRSDSIDESEEEGGDGKVCWQAAIFKVGDDCRQDMLALQIIGLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESALAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKQRFSPNSTEREAANFILKVIQNCFLSNRSRTYDMIQYYQNDIPY

Target ORF information:

RefSeq Version	NM_001251875.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphatidylinositol 4-kinase, catalytic, alpha (pi4ka), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251875.2

ORF Insert Sequence:

ATGTGCCCTG TCGATTATCA TGGGGTTTTT CAGCTGGATG AAAGGCGAAG AGATGCACTT 
ATTGCTTTAG GAATATTTCT GGTGGAGTCA GATCTTCAGC ACAAAGAGAC TGTAGTTCCA 
TACCTTTTGA GACTCTTGAG GGGACTTCCA AAGGTGCAGT GGATAGAGGA AAGCAATTCT 
CGTAAGGGAA AAGGGACACT TCCAGTGGCA GAAACCTTCA GCTTCTGTTT AGTTACACTA 
CTGTCGGATG TTGCGCACAG GGATCTGTCC TTCAGAGAGC AGATCTTAGA AGCTGTTTTG 
GATGTGATGC AGTGTCTGCT GGGAATGTGC CAGTCACCCC AAATTCAGGA TAAAGAATAC 
TTGTGTAAAT ATGCAGTTCC ATGCTTGATT GGAATCTCAC GGGCCTTTGG ACGATACAGC 
AACACTGAAG AGTCTCTTTT ATCCAAGCTG TTCCCCAAAG TTTCCCCACA GTCACTAAAG 
GCGCCAGAGG AATTAGAAGG AATACGTAGA CGTTCCTTTA ATGACTTCCG CTCCATCCTG 
CCAAGCTCAC TGCTTACAGT GTGCCAGGGG GATTCTCTAA AGCGGAAAGC CAGCAGTGTT 
TCAAGTGGCT CACAGGTTAG CCCTGATCGC ATTGTTCCTC CTCCAGGCTC ACCTGCCGGC 
TCTACTATTC CTTACTTTGA AGGGTCATAT TTGCCAGATG GCAGTGCTGT GGATCCTGAC 
TATTACCTTA CCACCATCAG CTCTAGTTTC TCAGTCTCAC CTCTCTTCAC TGGTGTCAAC 
AACAAGGAGT TTGATATGCC CCAAGATACT TTGCGTCAGC TACTCCATAT GGTGAAACAG 
ATTGTGGCAG ACCCATTCCT GAGTTCTTTG GATATGCTCA TCTCTGAAGT TACAGAAGCC 
AATCCTAGTG TGGACCTATA TTATAAAACA TTCAGTGATC CCCTCTACGT GGCAGTGTTT 
AAGATGCTCA GAGATACACT TTATAATGTT AAAGATCCCC ATACTTCATT TGTGAAGGAG 
ATTCATGACT TTGTGCTTGA ACAGTTTAAC AGCAGCCAGG GTGAGCTGCA GAAAATTCTA 
CATGATATGG ATCGACTGAA TAATGAACTC AGCCCCTTGA AATTACGTTG TCAGGCAAAT 
GCAGCATGTG TGGACCTTAT GGTGTGGGCT GTCAAAGATG AACAAGGTGC AGAAAATCTT 
TGCATCAAAC TATCTGAAAA GCTGCAGTCC AAGACATCTA GCAAAGTGAT TATAGCCCAT 
ATGCCACTTC TAATATGCTG TTTACAGGGG CTAGGGCGTC TTTGTGAGCG GTTTCCTGTA 
GTTGTCCATT CTGTGACGCC TTCGCTTCGA GATTTTCTTG TGATCCCATC TCCGGTGCTG 
GTGAAGCTTT ATAAATACCA TAGCCAATAT CAGACAGGTG CTAATGATAT AAAGATCAGT 
GTAACCAATG AGCATTCCGA ATCCACTATG AACATTATGT CTGGCAAGAA AAGCCAGGCA 
TCTATGTATG AACAGCTGCG GGATATTGCC ATAGATAACA TCTGCAGGTG TTTAAAAGCT 
GGGTTAACCA TAGATCCTGT CATTGTTGAA GCTTTTCTTG CCAGTTTATC AAATCGCTTG 
TATATATCTC AGGAGAATGA AAAGGATGCT CATTTGATAC CTGATCATAC AATCCGTGCG 
TTGGGGCACA TTGCTGTTGC TCTAAGGGAC ACCCCCAAAG TGATGCAACC CATCCTGCAG 
ATCCTTCAGC AGAAGTTCTG CCAGCCCCCT TCACAGCTGG ATGTGCTCAT TATAGACCAA 
CTAGGGTGCA TGGTGATCAC TGGCAATCAA TATATCTATC AAGAAGTGTG GAACCTTTTC 
CAACAGATTA GTGTGAAGGC CAGTTCAGTG GTATACTCTG CCACCAAGGA CTATAAAGAC 
CATGGCTACA GACACTGCTC CTTGGCTGTG ATTAACGCCC TGGCAAATAT TGCAGCCAAC 
ATCCAGGGAG AGCACCAGAT AGATGATTTG CTGATGAATT TGTTGGAGCT TTTTGTGCAG 
CTAGGACTAG AAGGAAAGAG AGCCAGTGAG AGGGCTAGCG AAAAGGGGCC TGCATTGAAG 
GCCTCCAGCA GTGCAGGAAA TCTGGGAGTG CTGATACCCG TAATTGCTGT GCTCACCCGA 
AGGCTTCCAC CAATAAAAGA GGCCAAGCCA CGACTTCAGA AACTCTTCAG AGATTTTTGG 
TTGTACTCTG TGCTGATGGG TTTTGCAGTG GAAGGATCAG GTCTCTGGCC TGAGGAATGG 
TATGAAGGTG TCTGTGAAAT TGCCACTAAA TCTCCTTTGC TGACGTTCCC CAGCAAAGAG 
CCTCTGCGCT CTGTACTGCA GTACAACTCT GCCATGAAAA ATGACACTGT AACGCCAGCA 
GAACTTAATG AGCTGAGAAG CACAATTATC AACCTTCTTG ATCCTGCTCC GGAAGTGTCT 
GCACTCATCA ACAAACTGGA CTTTGCCATG TCTACCTACC TGCTCTCTGT GTACCGCCTG 
GAGCACATGA GGGTTCTGCA TGCCACTGAT AAGGATCGAT TTCAAGTTAT GTTTTTTTAC 
TTCGAGGATA AAGCTATCCA AAAGGATAAA TCAGGTATGA TGCAGTGTGT TATTGCAGTA 
GCAGACAAAG TGTTTGAAGC TTTTCTGAAC GTGATGGCTG ATAAGCCTAA AACGAAAGAG 
AACGAGGAAG AATTGGAGCG CCATGCACAA TTCCTGCTGG TAAACTTTAA TCACATCCAC 
AAGCGCATTC GACGGGTCGC TGACAAATAC TTGTCAGGGC TAGTGGACAA ATTTCCCCAT 
TTGCTGTGGA GTGGTACTGT GCTGAAAACC ATGCTGGACA TACTGCAGAC TCTTTCCTTG 
TCTCTTAGTG CAGACATTCA CAAAGACCAG CCTTATTATG ACATTCCAGA TGCACCTTAT 
AGAATAACCG TTCCCGATAC CTATGAGGCC AGAGAGAGCA TTGTCAAAGA CTTTGCAGCT 
CGATGTGGCA TGATTCTTCA GGAAGCAATG AAATGGGCTC CTTCAGTGAC CAAGTCCCAC 
CTGCAGGAAT ATCTAAACAA GCACCAGAAC TGGATGTCTG GCCTTTCCCA GCACACTGGT 
CTTGCCATGG CTACTGAAAG CATACTGCAT TTTGCTGGGT ACAACAGGCA GAATACTACC 
TTGGGGTCTC CAGCTTCCTC TGCCCAGGTG ACACAACTAA CAGAAAGGCC AGCCTGTGTC 
AAGAAGGATT ATTCAAACTT CATGGCATCT CTCAACCTGC GCAACCGTTA TGCTGGAGAG 
GTTGCAGGCA TGATTGAGTT TTCTGATGCC ACTGGCCGAA CTTCTGACTT AAATAGGCTC 
ATGCTGAAGC ACCTGGGCGA TGCCTTGGAC TGCGGCCAAC CAGAGTCTTA TACACAGGTC 
ATGTTCAAAC TTACTGCGCT GCTCATTAGC AGCAAAGAGT GTGATGCTCA GCTTCTGCAC 
CACCTGTGCT GGGCACCCCT GCGGATGTTC ACTGATCATG GTATGGAGAC CGCTATTGCC 
TGCTGGGACT GGTTGCTTGC AGCTAAAAAT GGCATTGAAG TACCGTTTAT GCGTGAAATG 
GCTGGTGCTT GGCAAATGAC TGTAGAGCAA AAGATTGGAC TTTTCTCAGC AGAAAAGAAG 
GAGGCAGATC CTCTTGCTGC ATCTGAACAG AGCCAACCAC GTCCATGTGC ACCACAAGTG 
AACCCACATT ATATTTGGAT TGATTTTCTC ATGCAAAGGT TTGAGATTGC CAAATACTGC 
AGCTCTGATC AGGTGGAAAT CTTCTCCAGC CTTCTGCAGC GCTCCCTGTC CCTAAACATT 
GGTGGTTGTA AAGGAAGTCT TAACCGACAT GTGGCAGCTA TTGGCCCTCG ATTTAAGTTG 
CTGACTCTCG GGCTGTCCCT GTTACATGCA GACGTGGTCC CAAATGCGAC AATCCGGAAT 
GTCCTTAGAG AAAAGATCTA CTCTACAGCA TTTGATTATT TTAGTTGCCC TCCTAAGTTC 
CCGACTCAGA CGCAGAAGCT GCTAAGGGAG GATATCAGCA TCATGATCAA GTTCTGGACA 
GCCATGTTTT CTGATAAAAA ATATCTCACT GCCAATCAGC TAGTACCCCC AGATAATCAA 
GACCCTTCTG TAAACAGCTT GTCTGTGATG TCAGATGTGA ACCGCAGCAA TTTGGATGTG 
ACTGTTGGCT CCCGACAGCA AACAGCTCAG GGCTGGATCA ATACATACCC TCTGTCCAGT 
GGAATGTCCA CCATCTCTAA AAAATCTGGT TTAGCAAAGA AAACAAACCG TGGGACTCAG 
CTGCACAAGT ACTATATGAA GAGGCGGACT CTGTTGCTGT CACTTTTGGC CACTGAGATT 
GAACGCCTGA CCACATGGTA CAACCCCTTG TCCTCCCCTG AGTTGGAACT GGATCAGACA 
GGTGAAAACA GTGTGGCTAA CTGGAGATCT AAGTACATTA GTTTGAGTGA GAAGCAGTGG 
AAGGACAATG TGAACTTAGC ATGGAGCATC TCCCCTCACT TGGCTTTGCA ATTACCCACC 
AGGTTTAAGA ACACTGAGGC TATTGGGGGA GAAGTGACCC GCCTGGTTAG ATTGGACCCA 
GGAGCAGTGA GTGACACTCC AGAAGCAATA AAGTACTTGG TAACATGGCA CACAATAGAT 
GCTGATGCTC CTGAACTTAG TCACATACTC TGCTGGACAC CGACTGACCC ACCTACAGGA 
CTCTCCTACT TCTCTAGCAT GTACCCACCA CATCCACTTA CTGCGCAATA TGGCGTGAAA 
GTGCTGCGAT CATTCCCACC GGATGCAATC TTGTTCTACA TTCCGCAGAT AGTTCAAGCC 
TTGAGATATG ATAAGATGGG TTACGTTCGA GAATACATTC TATGGGCAGC TGCCAAATCA 
CAGCTTTTGG CTCATCAGTT TATTTGGAAC ATGAAGACAA ATATTTTCCT TGATGAGGAA 
GGGCACCAAA AAGACCCTGA TATTGGAGAA CTATTGGAGC AGCTGGTTGA AGAAATTACA 
GGATCTCTGT CTGGGCCAGC CAAGGATTTC TACCAGCGAG AATTTGACTT TTTCAATAAG 
ATCACAAATG TCTCTGCCAT CATTAAGCCA TTTCCAAAAG GAGAAGAACG GAAAAAGGCC 
TGTCTGAACT CTCTTTCAGA AGTAAAAGTG CAGCCAGGTT GTTACCTGCC CAGTAATCCA 
GAAGCCATTG TGTTGGACAT TGATTACACA TCTGGGACCC CTTTGCAGAG TGCTGCGAAG 
GCTCCTTATT TGGCCAAATT TAAGGTGAAG CGATGTGGAG TAAGTGAACT GGAGAAGGAA 
GGTCTCAGCT GCCGTTCAGA TTCTATTGAT GAGAGTGAGG AGGAAGGTGG AGATGGGAAG 
GTTTGCTGGC AAGCGGCTAT CTTTAAGGTC GGAGATGACT GCAGACAGGA TATGCTGGCT 
CTGCAGATCA TTGGTCTCTT TAAGAACATT TTCCAGCTGG TGGGACTTGA TCTCTTTGTT 
TTCCCATACA GAGTGGTAGC AACAGCTCCA GGGTGCGGAG TTATTGAATG TATACCAGAC 
TGCACCTCGC GGGACCAGCT CGGTCGCCAA ACAGATTTTG GAATGTATGA CTATTTTACT 
CGGCAGTATG GGGATGAATC AGCTCTTGCT TTCCAAAAGG CTCGATATAA CTTTATCCGA 
AGTATGGCTG CCTACAGCCT CCTGCTGTTC CTGCTCCAGA TTAAAGATCG ACATAATGGA 
AATATCATGT TGGATAAAAA GGGGCACATT ATCCACATTG ATTTTGGTTT CATGTTTGAA 
AGCTCCCCAG GGGGCAACCT GGGATGGGAG CCAGATATAA AGCTTACTGA TGAGATGGTG 
ATGATTATGG GGGGCAAGAT GGAAGCTACA CCTTTCAAAT GGTTCATGGA GATGTGCGTG 
CGTGGCTACT TAGCTGTAAG GCCGTACATG GATGCTGTGG TGTCCCTAGT CACTCTTATG 
CTGGACACAG GATTGCCATG TTTCCGTGGA CAAACCATTA AACTACTGAA GCAGAGGTTC 
AGCCCCAACT CAACAGAGAG GGAAGCAGCC AATTTCATCT TAAAGGTCAT TCAAAATTGC 
TTCCTCAGCA ACAGGAGCCG AACCTACGAT ATGATCCAGT ATTATCAGAA TGACATCCCC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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pi4ka cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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