GUCY2D cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCY2D
Entrez Gene ID	282245
Full Name	guanylate cyclase 2D, retinal
General protein information	Preferred Names guanylate cyclase 2D, retinal Names retinal guanylyl cyclase 1 GC-E RETGC-1 ROS-GC guanylate cyclase 2D, membrane (retina-specific) guanylate cyclase E rod outer segment membrane guanylate cyclase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010815985.2	XP_010814287.1	retinal guanylyl cyclase 1 isoform X3
XM_015458669.1	XP_015314155.1	retinal guanylyl cyclase 1 isoform X4
NM_174548.3	NP_776973.2	retinal guanylyl cyclase 1 precursor
XM_015458668.1	XP_015314154.1	retinal guanylyl cyclase 1 isoform X1
XM_010815983.2	XP_010814285.1	retinal guanylyl cyclase 1 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gucy2e	NP_077356.1
Xenopus tropicalis (tropical clawed frog)	gucy2d	XP_002942678.2
	GUCY2D	XP_001111670.1
Danio rerio (zebrafish)	gc3	NP_571941.1
Bos taurus (cattle)	GUCY2D	NP_776973.2
Homo sapiens (human)	GUCY2D	NP_000171.1
Pan troglodytes (chimpanzee)	GUCY2D	XP_003315414.1
Canis lupus familiaris (dog)	GUCY2D	NP_001003207.1
Mus musculus (house mouse)	Gucy2e	NP_032218.2

Gene Ontology
Bibliography

Related articles in PubMed

Bicarbonate Modulates Photoreceptor Guanylate Cyclase (ROS-GC) Catalytic Activity.
Duda T, Wen XH, Isayama T, Sharma RK, Makino CL
The Journal of biological chemistry290(17)11052-60(2015 Apr)

Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm.
Jankowska A, Burczy?ska B, Duda T, Warchol JB
Fertility and sterility93(3)904-12(2010 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The calcium-sensor guanylate cyclase activating protein type 2 specific site in rod outer segment membrane guanylate cyclase type 1.
Duda T, Fik-Rymarkiewicz E, Venkataraman V, Krishnan R, Koch KW, Sharma RK
Biochemistry44(19)7336-45(2005 May)

Guanylyl cyclase-activating proteins (GCAPs) are Ca2+/Mg2+ sensors: implications for photoreceptor guanylyl cyclase (RetGC) regulation in mammalian photoreceptors.
Peshenko IV, Dizhoor AM
The Journal of biological chemistry279(17)16903-6(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The findings define a new regulatory mechanism of the ROS-GC system that affects visual transduction and is likely to affect the course of retinal diseases caused by cGMP toxicity.
Title: Bicarbonate Modulates Photoreceptor Guanylate Cyclase (ROS-GC) Catalytic Activity.

The coexpression of ROS-GC1 and its activators in spermatozoa suggests that the Ca(2+)-modulated ROS-GC1 transduction system may be a part of the fertilization machinery
Title: Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm.

The Tyr965-Asn981 region of rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) houses both the structural and functional components that mediate guanylate cyclase activating protein type 2 (GCAP2) regulation.
Title: The calcium-sensor guanylate cyclase activating protein type 2 specific site in rod outer segment membrane guanylate cyclase type 1.

Mg(2+) binding to GCAPs is critical for setting the actual dynamic range of RetGC regulation by GCAPs at physiological levels of free Ca(2+)
Title: Guanylyl cyclase-activating proteins (GCAPs) are Ca2+/Mg2+ sensors: implications for photoreceptor guanylyl cyclase (RetGC) regulation in mammalian photoreceptors.

The following GUCY2D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCY2D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44479	NM_174548.3 Latest version!	Bos taurus guanylate cyclase 2D, retinal (GUCY2D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa45409	XM_010815983.2 Latest version!	Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa45410	XM_010815985.2 Latest version!	Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa93839	XM_015458668.1 Latest version!	Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa93840	XM_015458669.1 Latest version!	Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCY2D ( NM_174548.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776973.2 Bos taurus guanylate cyclase 2D, retinal (GUCY2D), mRNA.

Price & Availability↑

CloneID	OBa44479
Clone ID Related Accession (Same CDS sequence)	NM_174548.3
Accession Version	NM_174548.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	retinal guanylyl cyclase 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02048811.1. On Mar 30, 2010 this sequence version replaced NM_174548.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: U77095.1, L37089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145447 [ECO:0000348] ##Evidence-Data-END##

ATGACCGCCT GCACCTTCCT CGCGGGTGGG CTTCGGGACC CCGGACTCTG CGCCCCCACG 
CGGTGGTCTC CGTCTCCGCC CGGCCTTCCC CCGATCCCGC CCCGGCCCCG GCTTCGGCTG 
AGGCCACCGC TGCTGCTGCT GTTGCTCCTG CCGCGCTCCG TCCTCTCGGC CGTGTTCACT 
GTGGGGGTGC TGGGTCCCTG GGCCTGCGAC CCCATCTTCG CGCGTGCCCG CCCGGACCTG 
GCCGCCCGCC TGGCCGCCTC CCGCCTGAAC CACGCGGCTG CCCTGGAGGG CGGCCCCCGC 
TTCGAGGTCG CGCTGCTGCC CGAGCCGTGC CGGACGCCGG GCTCGCTGGG GGCCGTGTCC 
TCGGCGCTCA CCCGCGTCTC GGGTCTAGTT GGGCCGGTGA ACCCCGCAGC TTGCCGGCCG 
GCGGAATTAC TAGCCCAGGA GGCGGGGGTC GCTCTGGTCC CCTGGGGCTG TCCCGGGACG 
CGGGCAGCGG GCACCACGGC TCCGGTGGTG ACGCCCGCGG CCGATGCCCT CTACGCCCTC 
CTGCGCGCTT TCCGCTGGGC GCACGTGGCC TTGGTCACCG CCCCCCAGGA CCTGTGGGTG 
GAGGCGGGAC ACGCACTTTC CACGGCGCTC AGGGCCAGGG GCCTGCCCGT GGCCCTGGTG 
ACCTCCATGG AGCCCTCCGA CCTGTCTGGA GCCCGGGAGG CCTTGAGGAG GGTCCAAGAT 
GGGCCCAGAG TCAGAGCAGT GATCATGGTA ATGCACTCGG TCTTGCTGGG CGGCGAGGAG 
CAGCGCTGCC TGCTGGAGGC TGCAGAGGAA CTGGGCCTGG CCGACGGTTC CCTGGTCTTC 
CTGCCCTTCG ACACTCTCCA CTACGCCTTG TCTCCAGGCC CTGACGCCCT GGCGGTGCTC 
GCCAACAGCT CACAGCTTCG CAAGGCCCAC GATGCAGTGC TCACCCTCAC CCGTCACTGT 
CCCCTGGGAG GCAGCGTGCG GGACAGCCTG CGCAGGGCCC AAGAGCACCG GGAGCTGCCC 
CTGGACCTCA ATCTGCAGCA GGTATCCCCT CTCTTCGGCA CCATCTATGA CTCAGTCTTC 
TTGTTGGCTG GGGGCGTGGC GCGAGCACGG GTGGCCGCAG GTGGCGGCTG GGTGTCCGGA 
GCAGCGGTGG CCCGCCATAT CCGGGATGCC CGGGTCCCTG GCTTCTGTGG GGCCCTGGGA 
GGAGCTGAGG AGCCCTCGTT TGTGCTACTG GACACGGATG CCACTGGGGA CCAGCTGTTC 
GCTACATACG TTCTGGACCC TACGCAGGGC TTCTTCCACT CCGCTGGAAC CCCAGTGCAT 
TTTCCTAAAG GTGGACGGGG GCCTGGGCCT GACCCCTCGT GTTGGTTCGA CCCAGACACC 
ATCTGCAACG GAGGAGTGGA ACCCAGCGTC GTCTTTATTG GCTTCCTCCT GGTGGTTGGG 
ATGGGGCTGG CTGGGGCCTT CCTGGCCCAT TATTGTAGGC ACCGGCTGCT TCACATCCAA 
ATGGTCTCTG GCCCCAACAA GATCATCCTG ACTCTGGATG ACATCACCTT CCTCCACCCG 
CATGGGGGCA ACTCTCGAAA GGTGGCCCAG GGGAGTCGAA CCAGTCTGGC TGCCCGCAGC 
ATATCAGATG TTCGCAGCAT CCACAGCCAG CTCCCGGATT ACACCAACAT TGGCCTCTAT 
GAGGGAGACT GGGTTTGGCT GAAGAAATTC CCAGGAGATC GGCATATAGC TATCCGCCCA 
GCAACCAAGA TGGCCTTCTC CAAGATCCGG GAACTCCGGC ATGAAAACGT GGCCCTCTAC 
CTGGGCCTCT TCCTGGCGGG AGGAGCTGGC GGCCCCGCGG CCCCCGGGGA AGGCGTGCTG 
GCTGTGGTCT CGGAGCACTG CGCCCGCGGC TCCCTCCAAG ACCTCCTCGC CCAGAGAGAC 
ATAAAGCTGG ACTGGATGTT CAAGTCTTCC CTCCTCCTGG ACCTCATCAA GGGAATAAGG 
TATCTGCACC ATCGAGGGGT GGCCCATGGG CGGCTGAAGT CACGGAACTG CGTGGTGGAC 
GGGAGGTTCG TGCTTAAAGT CACCGACCAC GGTCATGGGC GACTGCTGGA GGCGCAGAGG 
GTGTTACCTG AGCCACCGAG CGCGGAGGAC CAGCTATGGA CAGCCCCGGA GCTGCTTCGA 
GACCCGGTCC TGGAGCGCCG GGGAACGCTG GCCGGCGACG TCTTCAGTCT GGGCATCATT 
ATGCAGGAGG TCGTGTGCCG CAGCGCCCCC TACGCCATGC TGGAGCTCAC TCCCGAGGAA 
GTGGTGAAGA GGGTACAGAG CCCCCCTCCA CTGTGTCGGC CCTCCGTGTC CATAGACCAG 
GCGCCCATGG AGTGCATCCA GCTGATGAAA CAGTGCTGGG CAGAGCAGCC AGAACTTCGG 
CCCTCCATGG ATCGCACCTT TGAACTGTTC AAGAGCATCA ACAAGGGCCG GAAGATGAAC 
ATCATCGACT CTATGCTGCG GATGCTGGAA CAGTACTCCA GTAACCTGGA GGACCTGATC 
CGGGAGCGCA CAGAGGAGCT GGAGCTGGAA AAGCAGAAGA CAGACCGGCT GCTCACACAG 
ATGCTGCCTC CGTCTGTGGC AGAGGCGCTG AAGATGGGGA CACCTGTGGA GCCCGAGTAT 
TTCGAAGAGG TGACACTGTA CTTCAGTGAC ATTGTGGGTT TCACCACCAT CTCAGCCATG 
AGTGAGCCCA TCGAAGTGGT AGACCTGCTC AACGACCTCT ACACACTCTT TGATGCCATC 
ATCGGTTCCC ATGATGTCTA CAAGGTGGAG ACAATTGGGG ATGCCTACAT GGTGGCCTCC 
GGCCTGCCCC AGAGGAACGG GCACCGGCAC GCGGCAGAGA TCGCCAATAT GGCTCTGGAC 
ATCCTCAGCG CCGTGGGCAC CTTCCGCATG CGCCACATGC CGGAAGTGCC CGTGCGCATC 
CGCATTGGCC TGCACTCGGG CCCGTGCGTG GCGGGCGTGG TGGGCCTCAC CATGCCCCGG 
TACTGCCTGT TTGGGGACAC AGTCAACACC GCTTCGCGCA TGGAGTCCAC CGGGCTGCCT 
TACCGCATCC ACGTGAACAG GAGCACCGTG CAGATCCTTT CCGCCCTAAA CGAGGGCTTC 
CTGACTGAGG TGCGGGGCCG CACGGAGCTG AAGGGCAAGG GCGCAGAGGA AACCTACTGG 
CTGGTGGGCA GACGCGGCTT CAACAAACCC ATCCCCAAAC CACCGGACCT GCAACCCGGG 
GCCAGCAACC ACGGCATCAG CCTGCACGAG ATCCCCCCTG ATCGGCGCCA GAAGCTAGAG 
AAGGCGAGGC CAGGCCAGTT TTCTGGGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776973.2
CDS	118..3450
Translation	MTACTFLAGGLRDPGLCAPTRWSPSPPGLPPIPPRPRLRLRPPLLLLLLLPRSVLSAVFTVGVLGPWACDPIFARARPDLAARLAASRLNHAAALEGGPRFEVALLPEPCRTPGSLGAVSSALTRVSGLVGPVNPAACRPAELLAQEAGVALVPWGCPGTRAAGTTAPVVTPAADALYALLRAFRWAHVALVTAPQDLWVEAGHALSTALRARGLPVALVTSMEPSDLSGAREALRRVQDGPRVRAVIMVMHSVLLGGEEQRCLLEAAEELGLADGSLVFLPFDTLHYALSPGPDALAVLANSSQLRKAHDAVLTLTRHCPLGGSVRDSLRRAQEHRELPLDLNLQQVSPLFGTIYDSVFLLAGGVARARVAAGGGWVSGAAVARHIRDARVPGFCGALGGAEEPSFVLLDTDATGDQLFATYVLDPTQGFFHSAGTPVHFPKGGRGPGPDPSCWFDPDTICNGGVEPSVVFIGFLLVVGMGLAGAFLAHYCRHRLLHIQMVSGPNKIILTLDDITFLHPHGGNSRKVAQGSRTSLAARSISDVRSIHSQLPDYTNIGLYEGDWVWLKKFPGDRHIAIRPATKMAFSKIRELRHENVALYLGLFLAGGAGGPAAPGEGVLAVVSEHCARGSLQDLLAQRDIKLDWMFKSSLLLDLIKGIRYLHHRGVAHGRLKSRNCVVDGRFVLKVTDHGHGRLLEAQRVLPEPPSAEDQLWTAPELLRDPVLERRGTLAGDVFSLGIIMQEVVCRSAPYAMLELTPEEVVKRVQSPPPLCRPSVSIDQAPMECIQLMKQCWAEQPELRPSMDRTFELFKSINKGRKMNIIDSMLRMLEQYSSNLEDLIRERTEELELEKQKTDRLLTQMLPPSVAEALKMGTPVEPEYFEEVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPQRNGHRHAAEIANMALDILSAVGTFRMRHMPEVPVRIRIGLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVNRSTVQILSALNEGFLTEVRGRTELKGKGAEETYWLVGRRGFNKPIPKPPDLQPGASNHGISLHEIPPDRRQKLEKARPGQFSGK

Target ORF information:

RefSeq Version	NM_174548.3
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase 2D, retinal (GUCY2D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174548.3

ORF Insert Sequence:

ATGACCGCCT GCACCTTCCT CGCGGGTGGG CTTCGGGACC CCGGACTCTG CGCCCCCACG 
CGGTGGTCTC CGTCTCCGCC CGGCCTTCCC CCGATCCCGC CCCGGCCCCG GCTTCGGCTG 
AGGCCACCGC TGCTGCTGCT GTTGCTCCTG CCGCGCTCCG TCCTCTCGGC CGTGTTCACT 
GTGGGGGTGC TGGGTCCCTG GGCCTGCGAC CCCATCTTCG CGCGTGCCCG CCCGGACCTG 
GCCGCCCGCC TGGCCGCCTC CCGCCTGAAC CACGCGGCTG CCCTGGAGGG CGGCCCCCGC 
TTCGAGGTCG CGCTGCTGCC CGAGCCGTGC CGGACGCCGG GCTCGCTGGG GGCCGTGTCC 
TCGGCGCTCA CCCGCGTCTC GGGTCTAGTT GGGCCGGTGA ACCCCGCAGC TTGCCGGCCG 
GCGGAATTAC TAGCCCAGGA GGCGGGGGTC GCTCTGGTCC CCTGGGGCTG TCCCGGGACG 
CGGGCAGCGG GCACCACGGC TCCGGTGGTG ACGCCCGCGG CCGATGCCCT CTACGCCCTC 
CTGCGCGCTT TCCGCTGGGC GCACGTGGCC TTGGTCACCG CCCCCCAGGA CCTGTGGGTG 
GAGGCGGGAC ACGCACTTTC CACGGCGCTC AGGGCCAGGG GCCTGCCCGT GGCCCTGGTG 
ACCTCCATGG AGCCCTCCGA CCTGTCTGGA GCCCGGGAGG CCTTGAGGAG GGTCCAAGAT 
GGGCCCAGAG TCAGAGCAGT GATCATGGTA ATGCACTCGG TCTTGCTGGG CGGCGAGGAG 
CAGCGCTGCC TGCTGGAGGC TGCAGAGGAA CTGGGCCTGG CCGACGGTTC CCTGGTCTTC 
CTGCCCTTCG ACACTCTCCA CTACGCCTTG TCTCCAGGCC CTGACGCCCT GGCGGTGCTC 
GCCAACAGCT CACAGCTTCG CAAGGCCCAC GATGCAGTGC TCACCCTCAC CCGTCACTGT 
CCCCTGGGAG GCAGCGTGCG GGACAGCCTG CGCAGGGCCC AAGAGCACCG GGAGCTGCCC 
CTGGACCTCA ATCTGCAGCA GGTATCCCCT CTCTTCGGCA CCATCTATGA CTCAGTCTTC 
TTGTTGGCTG GGGGCGTGGC GCGAGCACGG GTGGCCGCAG GTGGCGGCTG GGTGTCCGGA 
GCAGCGGTGG CCCGCCATAT CCGGGATGCC CGGGTCCCTG GCTTCTGTGG GGCCCTGGGA 
GGAGCTGAGG AGCCCTCGTT TGTGCTACTG GACACGGATG CCACTGGGGA CCAGCTGTTC 
GCTACATACG TTCTGGACCC TACGCAGGGC TTCTTCCACT CCGCTGGAAC CCCAGTGCAT 
TTTCCTAAAG GTGGACGGGG GCCTGGGCCT GACCCCTCGT GTTGGTTCGA CCCAGACACC 
ATCTGCAACG GAGGAGTGGA ACCCAGCGTC GTCTTTATTG GCTTCCTCCT GGTGGTTGGG 
ATGGGGCTGG CTGGGGCCTT CCTGGCCCAT TATTGTAGGC ACCGGCTGCT TCACATCCAA 
ATGGTCTCTG GCCCCAACAA GATCATCCTG ACTCTGGATG ACATCACCTT CCTCCACCCG 
CATGGGGGCA ACTCTCGAAA GGTGGCCCAG GGGAGTCGAA CCAGTCTGGC TGCCCGCAGC 
ATATCAGATG TTCGCAGCAT CCACAGCCAG CTCCCGGATT ACACCAACAT TGGCCTCTAT 
GAGGGAGACT GGGTTTGGCT GAAGAAATTC CCAGGAGATC GGCATATAGC TATCCGCCCA 
GCAACCAAGA TGGCCTTCTC CAAGATCCGG GAACTCCGGC ATGAAAACGT GGCCCTCTAC 
CTGGGCCTCT TCCTGGCGGG AGGAGCTGGC GGCCCCGCGG CCCCCGGGGA AGGCGTGCTG 
GCTGTGGTCT CGGAGCACTG CGCCCGCGGC TCCCTCCAAG ACCTCCTCGC CCAGAGAGAC 
ATAAAGCTGG ACTGGATGTT CAAGTCTTCC CTCCTCCTGG ACCTCATCAA GGGAATAAGG 
TATCTGCACC ATCGAGGGGT GGCCCATGGG CGGCTGAAGT CACGGAACTG CGTGGTGGAC 
GGGAGGTTCG TGCTTAAAGT CACCGACCAC GGTCATGGGC GACTGCTGGA GGCGCAGAGG 
GTGTTACCTG AGCCACCGAG CGCGGAGGAC CAGCTATGGA CAGCCCCGGA GCTGCTTCGA 
GACCCGGTCC TGGAGCGCCG GGGAACGCTG GCCGGCGACG TCTTCAGTCT GGGCATCATT 
ATGCAGGAGG TCGTGTGCCG CAGCGCCCCC TACGCCATGC TGGAGCTCAC TCCCGAGGAA 
GTGGTGAAGA GGGTACAGAG CCCCCCTCCA CTGTGTCGGC CCTCCGTGTC CATAGACCAG 
GCGCCCATGG AGTGCATCCA GCTGATGAAA CAGTGCTGGG CAGAGCAGCC AGAACTTCGG 
CCCTCCATGG ATCGCACCTT TGAACTGTTC AAGAGCATCA ACAAGGGCCG GAAGATGAAC 
ATCATCGACT CTATGCTGCG GATGCTGGAA CAGTACTCCA GTAACCTGGA GGACCTGATC 
CGGGAGCGCA CAGAGGAGCT GGAGCTGGAA AAGCAGAAGA CAGACCGGCT GCTCACACAG 
ATGCTGCCTC CGTCTGTGGC AGAGGCGCTG AAGATGGGGA CACCTGTGGA GCCCGAGTAT 
TTCGAAGAGG TGACACTGTA CTTCAGTGAC ATTGTGGGTT TCACCACCAT CTCAGCCATG 
AGTGAGCCCA TCGAAGTGGT AGACCTGCTC AACGACCTCT ACACACTCTT TGATGCCATC 
ATCGGTTCCC ATGATGTCTA CAAGGTGGAG ACAATTGGGG ATGCCTACAT GGTGGCCTCC 
GGCCTGCCCC AGAGGAACGG GCACCGGCAC GCGGCAGAGA TCGCCAATAT GGCTCTGGAC 
ATCCTCAGCG CCGTGGGCAC CTTCCGCATG CGCCACATGC CGGAAGTGCC CGTGCGCATC 
CGCATTGGCC TGCACTCGGG CCCGTGCGTG GCGGGCGTGG TGGGCCTCAC CATGCCCCGG 
TACTGCCTGT TTGGGGACAC AGTCAACACC GCTTCGCGCA TGGAGTCCAC CGGGCTGCCT 
TACCGCATCC ACGTGAACAG GAGCACCGTG CAGATCCTTT CCGCCCTAAA CGAGGGCTTC 
CTGACTGAGG TGCGGGGCCG CACGGAGCTG AAGGGCAAGG GCGCAGAGGA AACCTACTGG 
CTGGTGGGCA GACGCGGCTT CAACAAACCC ATCCCCAAAC CACCGGACCT GCAACCCGGG 
GCCAGCAACC ACGGCATCAG CCTGCACGAG ATCCCCCCTG ATCGGCGCCA GAAGCTAGAG 
AAGGCGAGGC CAGGCCAGTT TTCTGGGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY2D ( XM_010815983.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814285.1 Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45409
Clone ID Related Accession (Same CDS sequence)	XM_010815983.2
Accession Version	XM_010815983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	retinal guanylyl cyclase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACTCG GTTCGTGCTC GCAGAAGCCG GCAATGACCG CCTGCACCTT CCTCGCGGGT 
GGGCTTCGGG ACCCCGGACT CTGCGCCCCC ACGCGGTGGT CTCCGTCTCC GCCCGGCCTT 
CCCCCGATCC CGCCCCGGCC CCGGCTTCGG CTGAGGCCAC CGCTGCTGCT GCTGTTGCTC 
CTGCCGCGCT CCGTCCTCTC GGCCGTGTTC ACTGTGGGGG TGCTGGGTCC CTGGGCCTGC 
GACCCCATCT TCGCGCGTGC CCGCCCGGAC CTGGCCGCCC GCCTGGCCGC CTCCCGCCTG 
AACCACGCGG CTGCCCTGGA GGGCGGCCCC CGCTTCGAGG TCGCGCTGCT GCCCGAGCCG 
TGCCGGACGC CGGGCTCGCT GGGGGCCGTG TCCTCGGCGC TCACCCGCGT CTCGGGTCTA 
GTTGGGCCGG TGAACCCCGC AGCTTGCCGG CCGGCGGAAT TACTAGCCCA GGAGGCGGGG 
GTCGCTCTGG TCCCCTGGGG CTGTCCCGGG ACGCGGGCAG CGGGCACCAC GGCTCCGGTG 
GTGACGCCCG CGGCCGATGC CCTCTACGCC CTCCTGCGCG CTTTCCGCTG GGCGCACGTG 
GCCTTGGTCA CCGCCCCCCA GGACCTGTGG GTGGAGGCGG GACACGCACT TTCCACGGCG 
CTCAGGGCCA GGGGCCTGCC CGTGGCCCTG GTGACCTCCA TGGAGCCCTC CGACCTGTCT 
GGAGCCCGGG AGGCCTTGAG GAGGGTCCAA GATGGGCCCA GAGTCAGAGT GATCATGGTA 
ATGCACTCGG TCTTGCTGGG CGGCGAGGAG CAGCGCTGCC TGCTGGAGGC TGCAGAGGAA 
CTGGGCCTGG CCGACGGTTC CCTGGTCTTC CTGCCCTTCG ACACTCTCCA CTACGCCTTG 
TCTCCAGGCC CTGACGCCCT GGCGGTGCTC GCCAACAGCT CACAGCTTCG CAAGGCCCAC 
GATGCAGTGC TCACCCTCAC CCGTCACTGT CCCCTGGGAG GCAGCGTGCG GGACAGCCTG 
CGCAGGGCCC AAGAGCACCG GGAGCTGCCC CTGGACCTCA ATCTGCAGCA GGTATCCCCT 
CTCTTCGGCA CCATCTATGA CTCAGTCTTC TTGTTGGCTG GGGGCGTGGC GCGAGCACGG 
GTGGCCGCAG GTGGCGGCTG GGTGTCCGGA GCAGCGGTGG CCCGCCATAT CCGGGATGCC 
CGGGTCCCTG GCTTCTGTGG GGCCCTGGGA GGAGCTGAGG AGCCCTCGTT TGTGCTACTG 
GACACGGATG CCACTGGGGA CCAGCTGTTC GCTACATACG TTCTGGACCC TACGCAGGGC 
TTCTTCCACT CCGCTGGAAC CCCAGTGCAT TTTCCTAAAG GTGGACGGGG GCCTGGGCCT 
GACCCCTCGT GTTGGTTCGA CCCAGACACC ATCTGCAACG GAGGAGTGGA ACCCAGCGTC 
GTCTTTATTG GCTTCCTCCT GGTGGTTGGG ATGGGGCTGG CTGGGGCCTT CCTGGCCCAT 
TATTGTAGGC ACCGGCTGCT TCACATCCAA ATGGTCTCTG GCCCCAACAA GATCATCCTG 
ACTCTGGATG ACATCACCTT CCTCCACCCG CATGGGGGCA ACTCTCGAAA GGTGGCCCAG 
GGGAGTCGAA CCAGTCTGGC TGCCCGCAGC ATATCAGATG TTCGCAGCAT CCACAGCCAG 
CTCCCGGATT ACACCAACAT TGGCCTCTAT GAGGGAGACT GGGTTTGGCT GAAGAAATTC 
CCAGGAGATC GGCATATAGC TATCCGCCCA GCAACCAAGA TGGCCTTCTC CAAGATCCGG 
GAACTCCGGC ATGAAAACGT GGCCCTCTAC CTGGGCCTCT TCCTGGCGGG AGGAGCTGGC 
GGCCCCGCGG CCCCCGGGGA AGGCGTGCTG GCTGTGGTCT CGGAGCACTG CGCCCGCGGC 
TCCCTCCAAG ACCTCCTCGC CCAGAGAGAC ATAAAGCTGG ACTGGATGTT CAAGTCTTCC 
CTCCTCCTGG ACCTCATCAA GGGAATAAGG TATCTGCACC ATCGAGGGGT GGCCCATGGG 
CGGCTGAAGT CACGGAACTG CGTGGTGGAC GGGAGGTTCG TGCTTAAAGT CACCGACCAC 
GGTCATGGGC GACTGCTGGA GGCGCAGAGG GTGTTACCTG AGCCACCGAG CGCGGAGGAC 
CAGCTATGGA CAGCCCCGGA GCTGCTTCGA GACCCGGTCC TGGAGCGCCG GGGAACGCTG 
GCCGGCGACG TCTTCAGTCT GGGCATCATT ATGCAGGAGG TCGTGTGCCG CAGCGCCCCC 
TACGCCATGC TGGAGCTCAC TCCCGAGGAA GTGGTGAAGA GGGTACAGAG CCCCCCTCCA 
CTGTGTCGGC CCTCCGTGTC CATAGACCAG GCGCCCATGG AGTGCATCCA GCTGATGAAA 
CAGTGCTGGG CAGAGCAGCC AGAACTTCGG CCCTCCATGG ATCGCACCTT TGAACTGTTC 
AAGAGCATCA ACAAGGGCCG GAAGATGAAC ATCATCGACT CTATGCTGCG GATGCTGGAA 
CAGTACTCCA GTAACCTGGA GGACCTGATC CGGGAGCGCA CAGAGGAGCT GGAGCTGGAA 
AAGCAGAAGA CAGACCGGCT GCTCACACAG ATGCTGCCTC CGTCTGTGGC AGAGGCGCTG 
AAGATGGGGA CACCTGTGGA GCCCGAGTAT TTCGAAGAGG TGACACTGTA CTTCAGTGAC 
ATTGTGGGTT TCACCACCAT CTCAGCCATG AGTGAGCCCA TCGAAGTGGT AGACCTGCTC 
AACGACCTCT ACACACTCTT TGATGCCATC ATCGGTTCCC ATGATGTCTA CAAGGTGGAG 
ACAATTGGGG ATGCCTACAT GGTGGCCTCC GGCCTGCCCC AGAGGAACGG GCACCGGCAC 
GCGGCAGAGA TCGCCAATAT GGCTCTGGAC ATCCTCAGCG CCGTGGGCAC CTTCCGCATG 
CGCCACATGC CGGAAGTGCC CGTGCGCATC CGCATTGGCC TGCACTCGGG CCCGTGCGTG 
GCGGGCGTGG TGGGCCTCAC CATGCCCCGG TACTGCCTGT TTGGGGACAC AGTCAACACC 
GCTTCGCGCA TGGAGTCCAC CGGGCTGCCT TACCGCATCC ACGTGAACAG GAGCACCGTG 
CAGATCCTTT CCGCCCTAAA CGAGGGCTTC CTGACTGAGG TGCGGGGCCG CACGGAGCTG 
AAGGGCAAGG GCGCAGAGGA AACCTACTGG CTGGTGGGCA GACGCGGCTT CAACAAACCC 
ATCCCCAAAC CACCGGACCT GCAACCCGGG GCCAGCAACC ACGGCATCAG CCTGCACGAG 
ATCCCCCCTG ATCGGCGCCA GAAGCTAGAG AAGGCGAGGC CAGGCCAGTT TTCTGGGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814285.1
CDS	1123..4485
Translation	MLLGSCSQKPAMTACTFLAGGLRDPGLCAPTRWSPSPPGLPPIPPRPRLRLRPPLLLLLLLPRSVLSAVFTVGVLGPWACDPIFARARPDLAARLAASRLNHAAALEGGPRFEVALLPEPCRTPGSLGAVSSALTRVSGLVGPVNPAACRPAELLAQEAGVALVPWGCPGTRAAGTTAPVVTPAADALYALLRAFRWAHVALVTAPQDLWVEAGHALSTALRARGLPVALVTSMEPSDLSGAREALRRVQDGPRVRVIMVMHSVLLGGEEQRCLLEAAEELGLADGSLVFLPFDTLHYALSPGPDALAVLANSSQLRKAHDAVLTLTRHCPLGGSVRDSLRRAQEHRELPLDLNLQQVSPLFGTIYDSVFLLAGGVARARVAAGGGWVSGAAVARHIRDARVPGFCGALGGAEEPSFVLLDTDATGDQLFATYVLDPTQGFFHSAGTPVHFPKGGRGPGPDPSCWFDPDTICNGGVEPSVVFIGFLLVVGMGLAGAFLAHYCRHRLLHIQMVSGPNKIILTLDDITFLHPHGGNSRKVAQGSRTSLAARSISDVRSIHSQLPDYTNIGLYEGDWVWLKKFPGDRHIAIRPATKMAFSKIRELRHENVALYLGLFLAGGAGGPAAPGEGVLAVVSEHCARGSLQDLLAQRDIKLDWMFKSSLLLDLIKGIRYLHHRGVAHGRLKSRNCVVDGRFVLKVTDHGHGRLLEAQRVLPEPPSAEDQLWTAPELLRDPVLERRGTLAGDVFSLGIIMQEVVCRSAPYAMLELTPEEVVKRVQSPPPLCRPSVSIDQAPMECIQLMKQCWAEQPELRPSMDRTFELFKSINKGRKMNIIDSMLRMLEQYSSNLEDLIRERTEELELEKQKTDRLLTQMLPPSVAEALKMGTPVEPEYFEEVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPQRNGHRHAAEIANMALDILSAVGTFRMRHMPEVPVRIRIGLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVNRSTVQILSALNEGFLTEVRGRTELKGKGAEETYWLVGRRGFNKPIPKPPDLQPGASNHGISLHEIPPDRRQKLEKARPGQFSGK

Target ORF information:

RefSeq Version	XM_010815983.2
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815983.2

ORF Insert Sequence:

ATGCTACTCG GTTCGTGCTC GCAGAAGCCG GCAATGACCG CCTGCACCTT CCTCGCGGGT 
GGGCTTCGGG ACCCCGGACT CTGCGCCCCC ACGCGGTGGT CTCCGTCTCC GCCCGGCCTT 
CCCCCGATCC CGCCCCGGCC CCGGCTTCGG CTGAGGCCAC CGCTGCTGCT GCTGTTGCTC 
CTGCCGCGCT CCGTCCTCTC GGCCGTGTTC ACTGTGGGGG TGCTGGGTCC CTGGGCCTGC 
GACCCCATCT TCGCGCGTGC CCGCCCGGAC CTGGCCGCCC GCCTGGCCGC CTCCCGCCTG 
AACCACGCGG CTGCCCTGGA GGGCGGCCCC CGCTTCGAGG TCGCGCTGCT GCCCGAGCCG 
TGCCGGACGC CGGGCTCGCT GGGGGCCGTG TCCTCGGCGC TCACCCGCGT CTCGGGTCTA 
GTTGGGCCGG TGAACCCCGC AGCTTGCCGG CCGGCGGAAT TACTAGCCCA GGAGGCGGGG 
GTCGCTCTGG TCCCCTGGGG CTGTCCCGGG ACGCGGGCAG CGGGCACCAC GGCTCCGGTG 
GTGACGCCCG CGGCCGATGC CCTCTACGCC CTCCTGCGCG CTTTCCGCTG GGCGCACGTG 
GCCTTGGTCA CCGCCCCCCA GGACCTGTGG GTGGAGGCGG GACACGCACT TTCCACGGCG 
CTCAGGGCCA GGGGCCTGCC CGTGGCCCTG GTGACCTCCA TGGAGCCCTC CGACCTGTCT 
GGAGCCCGGG AGGCCTTGAG GAGGGTCCAA GATGGGCCCA GAGTCAGAGT GATCATGGTA 
ATGCACTCGG TCTTGCTGGG CGGCGAGGAG CAGCGCTGCC TGCTGGAGGC TGCAGAGGAA 
CTGGGCCTGG CCGACGGTTC CCTGGTCTTC CTGCCCTTCG ACACTCTCCA CTACGCCTTG 
TCTCCAGGCC CTGACGCCCT GGCGGTGCTC GCCAACAGCT CACAGCTTCG CAAGGCCCAC 
GATGCAGTGC TCACCCTCAC CCGTCACTGT CCCCTGGGAG GCAGCGTGCG GGACAGCCTG 
CGCAGGGCCC AAGAGCACCG GGAGCTGCCC CTGGACCTCA ATCTGCAGCA GGTATCCCCT 
CTCTTCGGCA CCATCTATGA CTCAGTCTTC TTGTTGGCTG GGGGCGTGGC GCGAGCACGG 
GTGGCCGCAG GTGGCGGCTG GGTGTCCGGA GCAGCGGTGG CCCGCCATAT CCGGGATGCC 
CGGGTCCCTG GCTTCTGTGG GGCCCTGGGA GGAGCTGAGG AGCCCTCGTT TGTGCTACTG 
GACACGGATG CCACTGGGGA CCAGCTGTTC GCTACATACG TTCTGGACCC TACGCAGGGC 
TTCTTCCACT CCGCTGGAAC CCCAGTGCAT TTTCCTAAAG GTGGACGGGG GCCTGGGCCT 
GACCCCTCGT GTTGGTTCGA CCCAGACACC ATCTGCAACG GAGGAGTGGA ACCCAGCGTC 
GTCTTTATTG GCTTCCTCCT GGTGGTTGGG ATGGGGCTGG CTGGGGCCTT CCTGGCCCAT 
TATTGTAGGC ACCGGCTGCT TCACATCCAA ATGGTCTCTG GCCCCAACAA GATCATCCTG 
ACTCTGGATG ACATCACCTT CCTCCACCCG CATGGGGGCA ACTCTCGAAA GGTGGCCCAG 
GGGAGTCGAA CCAGTCTGGC TGCCCGCAGC ATATCAGATG TTCGCAGCAT CCACAGCCAG 
CTCCCGGATT ACACCAACAT TGGCCTCTAT GAGGGAGACT GGGTTTGGCT GAAGAAATTC 
CCAGGAGATC GGCATATAGC TATCCGCCCA GCAACCAAGA TGGCCTTCTC CAAGATCCGG 
GAACTCCGGC ATGAAAACGT GGCCCTCTAC CTGGGCCTCT TCCTGGCGGG AGGAGCTGGC 
GGCCCCGCGG CCCCCGGGGA AGGCGTGCTG GCTGTGGTCT CGGAGCACTG CGCCCGCGGC 
TCCCTCCAAG ACCTCCTCGC CCAGAGAGAC ATAAAGCTGG ACTGGATGTT CAAGTCTTCC 
CTCCTCCTGG ACCTCATCAA GGGAATAAGG TATCTGCACC ATCGAGGGGT GGCCCATGGG 
CGGCTGAAGT CACGGAACTG CGTGGTGGAC GGGAGGTTCG TGCTTAAAGT CACCGACCAC 
GGTCATGGGC GACTGCTGGA GGCGCAGAGG GTGTTACCTG AGCCACCGAG CGCGGAGGAC 
CAGCTATGGA CAGCCCCGGA GCTGCTTCGA GACCCGGTCC TGGAGCGCCG GGGAACGCTG 
GCCGGCGACG TCTTCAGTCT GGGCATCATT ATGCAGGAGG TCGTGTGCCG CAGCGCCCCC 
TACGCCATGC TGGAGCTCAC TCCCGAGGAA GTGGTGAAGA GGGTACAGAG CCCCCCTCCA 
CTGTGTCGGC CCTCCGTGTC CATAGACCAG GCGCCCATGG AGTGCATCCA GCTGATGAAA 
CAGTGCTGGG CAGAGCAGCC AGAACTTCGG CCCTCCATGG ATCGCACCTT TGAACTGTTC 
AAGAGCATCA ACAAGGGCCG GAAGATGAAC ATCATCGACT CTATGCTGCG GATGCTGGAA 
CAGTACTCCA GTAACCTGGA GGACCTGATC CGGGAGCGCA CAGAGGAGCT GGAGCTGGAA 
AAGCAGAAGA CAGACCGGCT GCTCACACAG ATGCTGCCTC CGTCTGTGGC AGAGGCGCTG 
AAGATGGGGA CACCTGTGGA GCCCGAGTAT TTCGAAGAGG TGACACTGTA CTTCAGTGAC 
ATTGTGGGTT TCACCACCAT CTCAGCCATG AGTGAGCCCA TCGAAGTGGT AGACCTGCTC 
AACGACCTCT ACACACTCTT TGATGCCATC ATCGGTTCCC ATGATGTCTA CAAGGTGGAG 
ACAATTGGGG ATGCCTACAT GGTGGCCTCC GGCCTGCCCC AGAGGAACGG GCACCGGCAC 
GCGGCAGAGA TCGCCAATAT GGCTCTGGAC ATCCTCAGCG CCGTGGGCAC CTTCCGCATG 
CGCCACATGC CGGAAGTGCC CGTGCGCATC CGCATTGGCC TGCACTCGGG CCCGTGCGTG 
GCGGGCGTGG TGGGCCTCAC CATGCCCCGG TACTGCCTGT TTGGGGACAC AGTCAACACC 
GCTTCGCGCA TGGAGTCCAC CGGGCTGCCT TACCGCATCC ACGTGAACAG GAGCACCGTG 
CAGATCCTTT CCGCCCTAAA CGAGGGCTTC CTGACTGAGG TGCGGGGCCG CACGGAGCTG 
AAGGGCAAGG GCGCAGAGGA AACCTACTGG CTGGTGGGCA GACGCGGCTT CAACAAACCC 
ATCCCCAAAC CACCGGACCT GCAACCCGGG GCCAGCAACC ACGGCATCAG CCTGCACGAG 
ATCCCCCCTG ATCGGCGCCA GAAGCTAGAG AAGGCGAGGC CAGGCCAGTT TTCTGGGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY2D ( XM_010815985.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814287.1 Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X3, mRNA.

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CloneID	OBa45410
Clone ID Related Accession (Same CDS sequence)	XM_010815985.2
Accession Version	XM_010815985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	retinal guanylyl cyclase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACTCG GTTCGTGCTC GCAGAAGCCG GCAATGACCG CCTGCACCTT CCTCGCGGGT 
GGGCTTCGGG ACCCCGGACT CTGCGCCCCC ACGCGGTGGT CTCCGTCTCC GCCCGGCCTT 
CCCCCGATCC CGCCCCGGCC CCGGCTTCGG CTGAGGCCAC CGCTGCTGCT GCTGTTGCTC 
CTGCCGCGCT CCGTCCTCTC GGCCGTGTTC ACTGTGGGGG TGCTGGGTCC CTGGGCCTGC 
GACCCCATCT TCGCGCGTGC CCGCCCGGAC CTGGCCGCCC GCCTGGCCGC CTCCCGCCTG 
AACCACGCGG CTGCCCTGGA GGGCGGCCCC CGCTTCGAGG TCGCGCTGCT GCCCGAGCCG 
TGCCGGACGC CGGGCTCGCT GGGGGCCGTG TCCTCGGCGC TCACCCGCGT CTCGGGTCTA 
GTTGGGCCGG TGAACCCCGC AGCTTGCCGG CCGGCGGAAT TACTAGCCCA GGAGGCGGGG 
GTCGCTCTGG TCCCCTGGGG CTGTCCCGGG ACGCGGGCAG CGGGCACCAC GGCTCCGGTG 
GTGACGCCCG CGGCCGATGC CCTCTACGCC CTCCTGCGCG CTTTCCGCTG GGCGCACGTG 
GCCTTGGTCA CCGCCCCCCA GGACCTGTGG GTGGAGGCGG GACACGCACT TTCCACGGCG 
CTCAGGGCCA GGGGCCTGCC CGTGGCCCTG GTGACCTCCA TGGAGCCCTC CGACCTGTCT 
GGAGCCCGGG AGGCCTTGAG GAGGGTCCAA GATGGGCCCA GAGTCAGAGG CCCTGACGCC 
CTGGCGGTGC TCGCCAACAG CTCACAGCTT CGCAAGGCCC ACGATGCAGT GCTCACCCTC 
ACCCGTCACT GTCCCCTGGG AGGCAGCGTG CGGGACAGCC TGCGCAGGGC CCAAGAGCAC 
CGGGAGCTGC CCCTGGACCT CAATCTGCAG CAGGTATCCC CTCTCTTCGG CACCATCTAT 
GACTCAGTCT TCTTGTTGGC TGGGGGCGTG GCGCGAGCAC GGGTGGCCGC AGGTGGCGGC 
TGGGTGTCCG GAGCAGCGGT GGCCCGCCAT ATCCGGGATG CCCGGGTCCC TGGCTTCTGT 
GGGGCCCTGG GAGGAGCTGA GGAGCCCTCG TTTGTGCTAC TGGACACGGA TGCCACTGGG 
GACCAGCTGT TCGCTACATA CGTTCTGGAC CCTACGCAGG GCTTCTTCCA CTCCGCTGGA 
ACCCCAGTGC ATTTTCCTAA AGGTGGACGG GGGCCTGGGC CTGACCCCTC GTGTTGGTTC 
GACCCAGACA CCATCTGCAA CGGAGGAGTG GAACCCAGCG TCGTCTTTAT TGGCTTCCTC 
CTGGTGGTTG GGATGGGGCT GGCTGGGGCC TTCCTGGCCC ATTATTGTAG GCACCGGCTG 
CTTCACATCC AAATGGTCTC TGGCCCCAAC AAGATCATCC TGACTCTGGA TGACATCACC 
TTCCTCCACC CGCATGGGGG CAACTCTCGA AAGGTGGCCC AGGGGAGTCG AACCAGTCTG 
GCTGCCCGCA GCATATCAGA TGTTCGCAGC ATCCACAGCC AGCTCCCGGA TTACACCAAC 
ATTGGCCTCT ATGAGGGAGA CTGGGTTTGG CTGAAGAAAT TCCCAGGAGA TCGGCATATA 
GCTATCCGCC CAGCAACCAA GATGGCCTTC TCCAAGATCC GGGAACTCCG GCATGAAAAC 
GTGGCCCTCT ACCTGGGCCT CTTCCTGGCG GGAGGAGCTG GCGGCCCCGC GGCCCCCGGG 
GAAGGCGTGC TGGCTGTGGT CTCGGAGCAC TGCGCCCGCG GCTCCCTCCA AGACCTCCTC 
GCCCAGAGAG ACATAAAGCT GGACTGGATG TTCAAGTCTT CCCTCCTCCT GGACCTCATC 
AAGGGAATAA GGTATCTGCA CCATCGAGGG GTGGCCCATG GGCGGCTGAA GTCACGGAAC 
TGCGTGGTGG ACGGGAGGTT CGTGCTTAAA GTCACCGACC ACGGTCATGG GCGACTGCTG 
GAGGCGCAGA GGGTGTTACC TGAGCCACCG AGCGCGGAGG ACCAGCTATG GACAGCCCCG 
GAGCTGCTTC GAGACCCGGT CCTGGAGCGC CGGGGAACGC TGGCCGGCGA CGTCTTCAGT 
CTGGGCATCA TTATGCAGGA GGTCGTGTGC CGCAGCGCCC CCTACGCCAT GCTGGAGCTC 
ACTCCCGAGG AAGTGGTGAA GAGGGTACAG AGCCCCCCTC CACTGTGTCG GCCCTCCGTG 
TCCATAGACC AGGCGCCCAT GGAGTGCATC CAGCTGATGA AACAGTGCTG GGCAGAGCAG 
CCAGAACTTC GGCCCTCCAT GGATCGCACC TTTGAACTGT TCAAGAGCAT CAACAAGGGC 
CGGAAGATGA ACATCATCGA CTCTATGCTG CGGATGCTGG AACAGTACTC CAGTAACCTG 
GAGGACCTGA TCCGGGAGCG CACAGAGGAG CTGGAGCTGG AAAAGCAGAA GACAGACCGG 
CTGCTCACAC AGATGCTGCC TCCGTCTGTG GCAGAGGCGC TGAAGATGGG GACACCTGTG 
GAGCCCGAGT ATTTCGAAGA GGTGACACTG TACTTCAGTG ACATTGTGGG TTTCACCACC 
ATCTCAGCCA TGAGTGAGCC CATCGAAGTG GTAGACCTGC TCAACGACCT CTACACACTC 
TTTGATGCCA TCATCGGTTC CCATGATGTC TACAAGGTGG AGACAATTGG GGATGCCTAC 
ATGGTGGCCT CCGGCCTGCC CCAGAGGAAC GGGCACCGGC ACGCGGCAGA GATCGCCAAT 
ATGGCTCTGG ACATCCTCAG CGCCGTGGGC ACCTTCCGCA TGCGCCACAT GCCGGAAGTG 
CCCGTGCGCA TCCGCATTGG CCTGCACTCG GGCCCGTGCG TGGCGGGCGT GGTGGGCCTC 
ACCATGCCCC GGTACTGCCT GTTTGGGGAC ACAGTCAACA CCGCTTCGCG CATGGAGTCC 
ACCGGGCTGC CTTACCGCAT CCACGTGAAC AGGAGCACCG TGCAGATCCT TTCCGCCCTA 
AACGAGGGCT TCCTGACTGA GGTGCGGGGC CGCACGGAGC TGAAGGGCAA GGGCGCAGAG 
GAAACCTACT GGCTGGTGGG CAGACGCGGC TTCAACAAAC CCATCCCCAA ACCACCGGAC 
CTGCAACCCG GGGCCAGCAA CCACGGCATC AGCCTGCACG AGATCCCCCC TGATCGGCGC 
CAGAAGCTAG AGAAGGCGAG GCCAGGCCAG TTTTCTGGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814287.1
CDS	1123..4347
Translation	MLLGSCSQKPAMTACTFLAGGLRDPGLCAPTRWSPSPPGLPPIPPRPRLRLRPPLLLLLLLPRSVLSAVFTVGVLGPWACDPIFARARPDLAARLAASRLNHAAALEGGPRFEVALLPEPCRTPGSLGAVSSALTRVSGLVGPVNPAACRPAELLAQEAGVALVPWGCPGTRAAGTTAPVVTPAADALYALLRAFRWAHVALVTAPQDLWVEAGHALSTALRARGLPVALVTSMEPSDLSGAREALRRVQDGPRVRGPDALAVLANSSQLRKAHDAVLTLTRHCPLGGSVRDSLRRAQEHRELPLDLNLQQVSPLFGTIYDSVFLLAGGVARARVAAGGGWVSGAAVARHIRDARVPGFCGALGGAEEPSFVLLDTDATGDQLFATYVLDPTQGFFHSAGTPVHFPKGGRGPGPDPSCWFDPDTICNGGVEPSVVFIGFLLVVGMGLAGAFLAHYCRHRLLHIQMVSGPNKIILTLDDITFLHPHGGNSRKVAQGSRTSLAARSISDVRSIHSQLPDYTNIGLYEGDWVWLKKFPGDRHIAIRPATKMAFSKIRELRHENVALYLGLFLAGGAGGPAAPGEGVLAVVSEHCARGSLQDLLAQRDIKLDWMFKSSLLLDLIKGIRYLHHRGVAHGRLKSRNCVVDGRFVLKVTDHGHGRLLEAQRVLPEPPSAEDQLWTAPELLRDPVLERRGTLAGDVFSLGIIMQEVVCRSAPYAMLELTPEEVVKRVQSPPPLCRPSVSIDQAPMECIQLMKQCWAEQPELRPSMDRTFELFKSINKGRKMNIIDSMLRMLEQYSSNLEDLIRERTEELELEKQKTDRLLTQMLPPSVAEALKMGTPVEPEYFEEVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPQRNGHRHAAEIANMALDILSAVGTFRMRHMPEVPVRIRIGLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVNRSTVQILSALNEGFLTEVRGRTELKGKGAEETYWLVGRRGFNKPIPKPPDLQPGASNHGISLHEIPPDRRQKLEKARPGQFSGK

Target ORF information:

RefSeq Version	XM_010815985.2
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815985.2

ORF Insert Sequence:

ATGCTACTCG GTTCGTGCTC GCAGAAGCCG GCAATGACCG CCTGCACCTT CCTCGCGGGT 
GGGCTTCGGG ACCCCGGACT CTGCGCCCCC ACGCGGTGGT CTCCGTCTCC GCCCGGCCTT 
CCCCCGATCC CGCCCCGGCC CCGGCTTCGG CTGAGGCCAC CGCTGCTGCT GCTGTTGCTC 
CTGCCGCGCT CCGTCCTCTC GGCCGTGTTC ACTGTGGGGG TGCTGGGTCC CTGGGCCTGC 
GACCCCATCT TCGCGCGTGC CCGCCCGGAC CTGGCCGCCC GCCTGGCCGC CTCCCGCCTG 
AACCACGCGG CTGCCCTGGA GGGCGGCCCC CGCTTCGAGG TCGCGCTGCT GCCCGAGCCG 
TGCCGGACGC CGGGCTCGCT GGGGGCCGTG TCCTCGGCGC TCACCCGCGT CTCGGGTCTA 
GTTGGGCCGG TGAACCCCGC AGCTTGCCGG CCGGCGGAAT TACTAGCCCA GGAGGCGGGG 
GTCGCTCTGG TCCCCTGGGG CTGTCCCGGG ACGCGGGCAG CGGGCACCAC GGCTCCGGTG 
GTGACGCCCG CGGCCGATGC CCTCTACGCC CTCCTGCGCG CTTTCCGCTG GGCGCACGTG 
GCCTTGGTCA CCGCCCCCCA GGACCTGTGG GTGGAGGCGG GACACGCACT TTCCACGGCG 
CTCAGGGCCA GGGGCCTGCC CGTGGCCCTG GTGACCTCCA TGGAGCCCTC CGACCTGTCT 
GGAGCCCGGG AGGCCTTGAG GAGGGTCCAA GATGGGCCCA GAGTCAGAGG CCCTGACGCC 
CTGGCGGTGC TCGCCAACAG CTCACAGCTT CGCAAGGCCC ACGATGCAGT GCTCACCCTC 
ACCCGTCACT GTCCCCTGGG AGGCAGCGTG CGGGACAGCC TGCGCAGGGC CCAAGAGCAC 
CGGGAGCTGC CCCTGGACCT CAATCTGCAG CAGGTATCCC CTCTCTTCGG CACCATCTAT 
GACTCAGTCT TCTTGTTGGC TGGGGGCGTG GCGCGAGCAC GGGTGGCCGC AGGTGGCGGC 
TGGGTGTCCG GAGCAGCGGT GGCCCGCCAT ATCCGGGATG CCCGGGTCCC TGGCTTCTGT 
GGGGCCCTGG GAGGAGCTGA GGAGCCCTCG TTTGTGCTAC TGGACACGGA TGCCACTGGG 
GACCAGCTGT TCGCTACATA CGTTCTGGAC CCTACGCAGG GCTTCTTCCA CTCCGCTGGA 
ACCCCAGTGC ATTTTCCTAA AGGTGGACGG GGGCCTGGGC CTGACCCCTC GTGTTGGTTC 
GACCCAGACA CCATCTGCAA CGGAGGAGTG GAACCCAGCG TCGTCTTTAT TGGCTTCCTC 
CTGGTGGTTG GGATGGGGCT GGCTGGGGCC TTCCTGGCCC ATTATTGTAG GCACCGGCTG 
CTTCACATCC AAATGGTCTC TGGCCCCAAC AAGATCATCC TGACTCTGGA TGACATCACC 
TTCCTCCACC CGCATGGGGG CAACTCTCGA AAGGTGGCCC AGGGGAGTCG AACCAGTCTG 
GCTGCCCGCA GCATATCAGA TGTTCGCAGC ATCCACAGCC AGCTCCCGGA TTACACCAAC 
ATTGGCCTCT ATGAGGGAGA CTGGGTTTGG CTGAAGAAAT TCCCAGGAGA TCGGCATATA 
GCTATCCGCC CAGCAACCAA GATGGCCTTC TCCAAGATCC GGGAACTCCG GCATGAAAAC 
GTGGCCCTCT ACCTGGGCCT CTTCCTGGCG GGAGGAGCTG GCGGCCCCGC GGCCCCCGGG 
GAAGGCGTGC TGGCTGTGGT CTCGGAGCAC TGCGCCCGCG GCTCCCTCCA AGACCTCCTC 
GCCCAGAGAG ACATAAAGCT GGACTGGATG TTCAAGTCTT CCCTCCTCCT GGACCTCATC 
AAGGGAATAA GGTATCTGCA CCATCGAGGG GTGGCCCATG GGCGGCTGAA GTCACGGAAC 
TGCGTGGTGG ACGGGAGGTT CGTGCTTAAA GTCACCGACC ACGGTCATGG GCGACTGCTG 
GAGGCGCAGA GGGTGTTACC TGAGCCACCG AGCGCGGAGG ACCAGCTATG GACAGCCCCG 
GAGCTGCTTC GAGACCCGGT CCTGGAGCGC CGGGGAACGC TGGCCGGCGA CGTCTTCAGT 
CTGGGCATCA TTATGCAGGA GGTCGTGTGC CGCAGCGCCC CCTACGCCAT GCTGGAGCTC 
ACTCCCGAGG AAGTGGTGAA GAGGGTACAG AGCCCCCCTC CACTGTGTCG GCCCTCCGTG 
TCCATAGACC AGGCGCCCAT GGAGTGCATC CAGCTGATGA AACAGTGCTG GGCAGAGCAG 
CCAGAACTTC GGCCCTCCAT GGATCGCACC TTTGAACTGT TCAAGAGCAT CAACAAGGGC 
CGGAAGATGA ACATCATCGA CTCTATGCTG CGGATGCTGG AACAGTACTC CAGTAACCTG 
GAGGACCTGA TCCGGGAGCG CACAGAGGAG CTGGAGCTGG AAAAGCAGAA GACAGACCGG 
CTGCTCACAC AGATGCTGCC TCCGTCTGTG GCAGAGGCGC TGAAGATGGG GACACCTGTG 
GAGCCCGAGT ATTTCGAAGA GGTGACACTG TACTTCAGTG ACATTGTGGG TTTCACCACC 
ATCTCAGCCA TGAGTGAGCC CATCGAAGTG GTAGACCTGC TCAACGACCT CTACACACTC 
TTTGATGCCA TCATCGGTTC CCATGATGTC TACAAGGTGG AGACAATTGG GGATGCCTAC 
ATGGTGGCCT CCGGCCTGCC CCAGAGGAAC GGGCACCGGC ACGCGGCAGA GATCGCCAAT 
ATGGCTCTGG ACATCCTCAG CGCCGTGGGC ACCTTCCGCA TGCGCCACAT GCCGGAAGTG 
CCCGTGCGCA TCCGCATTGG CCTGCACTCG GGCCCGTGCG TGGCGGGCGT GGTGGGCCTC 
ACCATGCCCC GGTACTGCCT GTTTGGGGAC ACAGTCAACA CCGCTTCGCG CATGGAGTCC 
ACCGGGCTGC CTTACCGCAT CCACGTGAAC AGGAGCACCG TGCAGATCCT TTCCGCCCTA 
AACGAGGGCT TCCTGACTGA GGTGCGGGGC CGCACGGAGC TGAAGGGCAA GGGCGCAGAG 
GAAACCTACT GGCTGGTGGG CAGACGCGGC TTCAACAAAC CCATCCCCAA ACCACCGGAC 
CTGCAACCCG GGGCCAGCAA CCACGGCATC AGCCTGCACG AGATCCCCCC TGATCGGCGC 
CAGAAGCTAG AGAAGGCGAG GCCAGGCCAG TTTTCTGGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY2D ( XM_015458668.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314154.1 Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X1, mRNA.

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CloneID	OBa93839
Clone ID Related Accession (Same CDS sequence)	XM_015458668.1
Accession Version	XM_015458668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	retinal guanylyl cyclase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACTCG GTTCGTGCTC GCAGAAGCCG GCAATGACCG CCTGCACCTT CCTCGCGGGT 
GGGCTTCGGG ACCCCGGACT CTGCGCCCCC ACGCGGTGGT CTCCGTCTCC GCCCGGCCTT 
CCCCCGATCC CGCCCCGGCC CCGGCTTCGG CTGAGGCCAC CGCTGCTGCT GCTGTTGCTC 
CTGCCGCGCT CCGTCCTCTC GGCCGTGTTC ACTGTGGGGG TGCTGGGTCC CTGGGCCTGC 
GACCCCATCT TCGCGCGTGC CCGCCCGGAC CTGGCCGCCC GCCTGGCCGC CTCCCGCCTG 
AACCACGCGG CTGCCCTGGA GGGCGGCCCC CGCTTCGAGG TCGCGCTGCT GCCCGAGCCG 
TGCCGGACGC CGGGCTCGCT GGGGGCCGTG TCCTCGGCGC TCACCCGCGT CTCGGGTCTA 
GTTGGGCCGG TGAACCCCGC AGCTTGCCGG CCGGCGGAAT TACTAGCCCA GGAGGCGGGG 
GTCGCTCTGG TCCCCTGGGG CTGTCCCGGG ACGCGGGCAG CGGGCACCAC GGCTCCGGTG 
GTGACGCCCG CGGCCGATGC CCTCTACGCC CTCCTGCGCG CTTTCCGCTG GGCGCACGTG 
GCCTTGGTCA CCGCCCCCCA GGACCTGTGG GTGGAGGCGG GACACGCACT TTCCACGGCG 
CTCAGGGCCA GGGGCCTGCC CGTGGCCCTG GTGACCTCCA TGGAGCCCTC CGACCTGTCT 
GGAGCCCGGG AGGCCTTGAG GAGGGTCCAA GATGGGCCCA GAGTCAGAGC AGTGATCATG 
GTAATGCACT CGGTCTTGCT GGGCGGCGAG GAGCAGCGCT GCCTGCTGGA GGCTGCAGAG 
GAACTGGGCC TGGCCGACGG TTCCCTGGTC TTCCTGCCCT TCGACACTCT CCACTACGCC 
TTGTCTCCAG GCCCTGACGC CCTGGCGGTG CTCGCCAACA GCTCACAGCT TCGCAAGGCC 
CACGATGCAG TGCTCACCCT CACCCGTCAC TGTCCCCTGG GAGGCAGCGT GCGGGACAGC 
CTGCGCAGGG CCCAAGAGCA CCGGGAGCTG CCCCTGGACC TCAATCTGCA GCAGGTATCC 
CCTCTCTTCG GCACCATCTA TGACTCAGTC TTCTTGTTGG CTGGGGGCGT GGCGCGAGCA 
CGGGTGGCCG CAGGTGGCGG CTGGGTGTCC GGAGCAGCGG TGGCCCGCCA TATCCGGGAT 
GCCCGGGTCC CTGGCTTCTG TGGGGCCCTG GGAGGAGCTG AGGAGCCCTC GTTTGTGCTA 
CTGGACACGG ATGCCACTGG GGACCAGCTG TTCGCTACAT ACGTTCTGGA CCCTACGCAG 
GGCTTCTTCC ACTCCGCTGG AACCCCAGTG CATTTTCCTA AAGGTGGACG GGGGCCTGGG 
CCTGACCCCT CGTGTTGGTT CGACCCAGAC ACCATCTGCA ACGGAGGAGT GGAACCCAGC 
GTCGTCTTTA TTGGCTTCCT CCTGGTGGTT GGGATGGGGC TGGCTGGGGC CTTCCTGGCC 
CATTATTGTA GGCACCGGCT GCTTCACATC CAAATGGTCT CTGGCCCCAA CAAGATCATC 
CTGACTCTGG ATGACATCAC CTTCCTCCAC CCGCATGGGG GCAACTCTCG AAAGGTGGCC 
CAGGGGAGTC GAACCAGTCT GGCTGCCCGC AGCATATCAG ATGTTCGCAG CATCCACAGC 
CAGCTCCCGG ATTACACCAA CATTGGCCTC TATGAGGGAG ACTGGGTTTG GCTGAAGAAA 
TTCCCAGGAG ATCGGCATAT AGCTATCCGC CCAGCAACCA AGATGGCCTT CTCCAAGATC 
CGGGAACTCC GGCATGAAAA CGTGGCCCTC TACCTGGGCC TCTTCCTGGC GGGAGGAGCT 
GGCGGCCCCG CGGCCCCCGG GGAAGGCGTG CTGGCTGTGG TCTCGGAGCA CTGCGCCCGC 
GGCTCCCTCC AAGACCTCCT CGCCCAGAGA GACATAAAGC TGGACTGGAT GTTCAAGTCT 
TCCCTCCTCC TGGACCTCAT CAAGGGAATA AGGTATCTGC ACCATCGAGG GGTGGCCCAT 
GGGCGGCTGA AGTCACGGAA CTGCGTGGTG GACGGGAGGT TCGTGCTTAA AGTCACCGAC 
CACGGTCATG GGCGACTGCT GGAGGCGCAG AGGGTGTTAC CTGAGCCACC GAGCGCGGAG 
GACCAGCTAT GGACAGCCCC GGAGCTGCTT CGAGACCCGG TCCTGGAGCG CCGGGGAACG 
CTGGCCGGCG ACGTCTTCAG TCTGGGCATC ATTATGCAGG AGGTCGTGTG CCGCAGCGCC 
CCCTACGCCA TGCTGGAGCT CACTCCCGAG GAAGTGGTGA AGAGGGTACA GAGCCCCCCT 
CCACTGTGTC GGCCCTCCGT GTCCATAGAC CAGGCGCCCA TGGAGTGCAT CCAGCTGATG 
AAACAGTGCT GGGCAGAGCA GCCAGAACTT CGGCCCTCCA TGGATCGCAC CTTTGAACTG 
TTCAAGAGCA TCAACAAGGG CCGGAAGATG AACATCATCG ACTCTATGCT GCGGATGCTG 
GAACAGTACT CCAGTAACCT GGAGGACCTG ATCCGGGAGC GCACAGAGGA GCTGGAGCTG 
GAAAAGCAGA AGACAGACCG GCTGCTCACA CAGATGCTGC CTCCGTCTGT GGCAGAGGCG 
CTGAAGATGG GGACACCTGT GGAGCCCGAG TATTTCGAAG AGGTGACACT GTACTTCAGT 
GACATTGTGG GTTTCACCAC CATCTCAGCC ATGAGTGAGC CCATCGAAGT GGTAGACCTG 
CTCAACGACC TCTACACACT CTTTGATGCC ATCATCGGTT CCCATGATGT CTACAAGGTG 
GAGACAATTG GGGATGCCTA CATGGTGGCC TCCGGCCTGC CCCAGAGGAA CGGGCACCGG 
CACGCGGCAG AGATCGCCAA TATGGCTCTG GACATCCTCA GCGCCGTGGG CACCTTCCGC 
ATGCGCCACA TGCCGGAAGT GCCCGTGCGC ATCCGCATTG GCCTGCACTC GGGCCCGTGC 
GTGGCGGGCG TGGTGGGCCT CACCATGCCC CGGTACTGCC TGTTTGGGGA CACAGTCAAC 
ACCGCTTCGC GCATGGAGTC CACCGGGCTG CCTTACCGCA TCCACGTGAA CAGGAGCACC 
GTGCAGATCC TTTCCGCCCT AAACGAGGGC TTCCTGACTG AGGTGCGGGG CCGCACGGAG 
CTGAAGGGCA AGGGCGCAGA GGAAACCTAC TGGCTGGTGG GCAGACGCGG CTTCAACAAA 
CCCATCCCCA AACCACCGGA CCTGCAACCC GGGGCCAGCA ACCACGGCAT CAGCCTGCAC 
GAGATCCCCC CTGATCGGCG CCAGAAGCTA GAGAAGGCGA GGCCAGGCCA GTTTTCTGGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314154.1
CDS	1123..4488
Translation	MLLGSCSQKPAMTACTFLAGGLRDPGLCAPTRWSPSPPGLPPIPPRPRLRLRPPLLLLLLLPRSVLSAVFTVGVLGPWACDPIFARARPDLAARLAASRLNHAAALEGGPRFEVALLPEPCRTPGSLGAVSSALTRVSGLVGPVNPAACRPAELLAQEAGVALVPWGCPGTRAAGTTAPVVTPAADALYALLRAFRWAHVALVTAPQDLWVEAGHALSTALRARGLPVALVTSMEPSDLSGAREALRRVQDGPRVRAVIMVMHSVLLGGEEQRCLLEAAEELGLADGSLVFLPFDTLHYALSPGPDALAVLANSSQLRKAHDAVLTLTRHCPLGGSVRDSLRRAQEHRELPLDLNLQQVSPLFGTIYDSVFLLAGGVARARVAAGGGWVSGAAVARHIRDARVPGFCGALGGAEEPSFVLLDTDATGDQLFATYVLDPTQGFFHSAGTPVHFPKGGRGPGPDPSCWFDPDTICNGGVEPSVVFIGFLLVVGMGLAGAFLAHYCRHRLLHIQMVSGPNKIILTLDDITFLHPHGGNSRKVAQGSRTSLAARSISDVRSIHSQLPDYTNIGLYEGDWVWLKKFPGDRHIAIRPATKMAFSKIRELRHENVALYLGLFLAGGAGGPAAPGEGVLAVVSEHCARGSLQDLLAQRDIKLDWMFKSSLLLDLIKGIRYLHHRGVAHGRLKSRNCVVDGRFVLKVTDHGHGRLLEAQRVLPEPPSAEDQLWTAPELLRDPVLERRGTLAGDVFSLGIIMQEVVCRSAPYAMLELTPEEVVKRVQSPPPLCRPSVSIDQAPMECIQLMKQCWAEQPELRPSMDRTFELFKSINKGRKMNIIDSMLRMLEQYSSNLEDLIRERTEELELEKQKTDRLLTQMLPPSVAEALKMGTPVEPEYFEEVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPQRNGHRHAAEIANMALDILSAVGTFRMRHMPEVPVRIRIGLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVNRSTVQILSALNEGFLTEVRGRTELKGKGAEETYWLVGRRGFNKPIPKPPDLQPGASNHGISLHEIPPDRRQKLEKARPGQFSGK

Target ORF information:

RefSeq Version	XM_015458668.1
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458668.1

ORF Insert Sequence:

ATGCTACTCG GTTCGTGCTC GCAGAAGCCG GCAATGACCG CCTGCACCTT CCTCGCGGGT 
GGGCTTCGGG ACCCCGGACT CTGCGCCCCC ACGCGGTGGT CTCCGTCTCC GCCCGGCCTT 
CCCCCGATCC CGCCCCGGCC CCGGCTTCGG CTGAGGCCAC CGCTGCTGCT GCTGTTGCTC 
CTGCCGCGCT CCGTCCTCTC GGCCGTGTTC ACTGTGGGGG TGCTGGGTCC CTGGGCCTGC 
GACCCCATCT TCGCGCGTGC CCGCCCGGAC CTGGCCGCCC GCCTGGCCGC CTCCCGCCTG 
AACCACGCGG CTGCCCTGGA GGGCGGCCCC CGCTTCGAGG TCGCGCTGCT GCCCGAGCCG 
TGCCGGACGC CGGGCTCGCT GGGGGCCGTG TCCTCGGCGC TCACCCGCGT CTCGGGTCTA 
GTTGGGCCGG TGAACCCCGC AGCTTGCCGG CCGGCGGAAT TACTAGCCCA GGAGGCGGGG 
GTCGCTCTGG TCCCCTGGGG CTGTCCCGGG ACGCGGGCAG CGGGCACCAC GGCTCCGGTG 
GTGACGCCCG CGGCCGATGC CCTCTACGCC CTCCTGCGCG CTTTCCGCTG GGCGCACGTG 
GCCTTGGTCA CCGCCCCCCA GGACCTGTGG GTGGAGGCGG GACACGCACT TTCCACGGCG 
CTCAGGGCCA GGGGCCTGCC CGTGGCCCTG GTGACCTCCA TGGAGCCCTC CGACCTGTCT 
GGAGCCCGGG AGGCCTTGAG GAGGGTCCAA GATGGGCCCA GAGTCAGAGC AGTGATCATG 
GTAATGCACT CGGTCTTGCT GGGCGGCGAG GAGCAGCGCT GCCTGCTGGA GGCTGCAGAG 
GAACTGGGCC TGGCCGACGG TTCCCTGGTC TTCCTGCCCT TCGACACTCT CCACTACGCC 
TTGTCTCCAG GCCCTGACGC CCTGGCGGTG CTCGCCAACA GCTCACAGCT TCGCAAGGCC 
CACGATGCAG TGCTCACCCT CACCCGTCAC TGTCCCCTGG GAGGCAGCGT GCGGGACAGC 
CTGCGCAGGG CCCAAGAGCA CCGGGAGCTG CCCCTGGACC TCAATCTGCA GCAGGTATCC 
CCTCTCTTCG GCACCATCTA TGACTCAGTC TTCTTGTTGG CTGGGGGCGT GGCGCGAGCA 
CGGGTGGCCG CAGGTGGCGG CTGGGTGTCC GGAGCAGCGG TGGCCCGCCA TATCCGGGAT 
GCCCGGGTCC CTGGCTTCTG TGGGGCCCTG GGAGGAGCTG AGGAGCCCTC GTTTGTGCTA 
CTGGACACGG ATGCCACTGG GGACCAGCTG TTCGCTACAT ACGTTCTGGA CCCTACGCAG 
GGCTTCTTCC ACTCCGCTGG AACCCCAGTG CATTTTCCTA AAGGTGGACG GGGGCCTGGG 
CCTGACCCCT CGTGTTGGTT CGACCCAGAC ACCATCTGCA ACGGAGGAGT GGAACCCAGC 
GTCGTCTTTA TTGGCTTCCT CCTGGTGGTT GGGATGGGGC TGGCTGGGGC CTTCCTGGCC 
CATTATTGTA GGCACCGGCT GCTTCACATC CAAATGGTCT CTGGCCCCAA CAAGATCATC 
CTGACTCTGG ATGACATCAC CTTCCTCCAC CCGCATGGGG GCAACTCTCG AAAGGTGGCC 
CAGGGGAGTC GAACCAGTCT GGCTGCCCGC AGCATATCAG ATGTTCGCAG CATCCACAGC 
CAGCTCCCGG ATTACACCAA CATTGGCCTC TATGAGGGAG ACTGGGTTTG GCTGAAGAAA 
TTCCCAGGAG ATCGGCATAT AGCTATCCGC CCAGCAACCA AGATGGCCTT CTCCAAGATC 
CGGGAACTCC GGCATGAAAA CGTGGCCCTC TACCTGGGCC TCTTCCTGGC GGGAGGAGCT 
GGCGGCCCCG CGGCCCCCGG GGAAGGCGTG CTGGCTGTGG TCTCGGAGCA CTGCGCCCGC 
GGCTCCCTCC AAGACCTCCT CGCCCAGAGA GACATAAAGC TGGACTGGAT GTTCAAGTCT 
TCCCTCCTCC TGGACCTCAT CAAGGGAATA AGGTATCTGC ACCATCGAGG GGTGGCCCAT 
GGGCGGCTGA AGTCACGGAA CTGCGTGGTG GACGGGAGGT TCGTGCTTAA AGTCACCGAC 
CACGGTCATG GGCGACTGCT GGAGGCGCAG AGGGTGTTAC CTGAGCCACC GAGCGCGGAG 
GACCAGCTAT GGACAGCCCC GGAGCTGCTT CGAGACCCGG TCCTGGAGCG CCGGGGAACG 
CTGGCCGGCG ACGTCTTCAG TCTGGGCATC ATTATGCAGG AGGTCGTGTG CCGCAGCGCC 
CCCTACGCCA TGCTGGAGCT CACTCCCGAG GAAGTGGTGA AGAGGGTACA GAGCCCCCCT 
CCACTGTGTC GGCCCTCCGT GTCCATAGAC CAGGCGCCCA TGGAGTGCAT CCAGCTGATG 
AAACAGTGCT GGGCAGAGCA GCCAGAACTT CGGCCCTCCA TGGATCGCAC CTTTGAACTG 
TTCAAGAGCA TCAACAAGGG CCGGAAGATG AACATCATCG ACTCTATGCT GCGGATGCTG 
GAACAGTACT CCAGTAACCT GGAGGACCTG ATCCGGGAGC GCACAGAGGA GCTGGAGCTG 
GAAAAGCAGA AGACAGACCG GCTGCTCACA CAGATGCTGC CTCCGTCTGT GGCAGAGGCG 
CTGAAGATGG GGACACCTGT GGAGCCCGAG TATTTCGAAG AGGTGACACT GTACTTCAGT 
GACATTGTGG GTTTCACCAC CATCTCAGCC ATGAGTGAGC CCATCGAAGT GGTAGACCTG 
CTCAACGACC TCTACACACT CTTTGATGCC ATCATCGGTT CCCATGATGT CTACAAGGTG 
GAGACAATTG GGGATGCCTA CATGGTGGCC TCCGGCCTGC CCCAGAGGAA CGGGCACCGG 
CACGCGGCAG AGATCGCCAA TATGGCTCTG GACATCCTCA GCGCCGTGGG CACCTTCCGC 
ATGCGCCACA TGCCGGAAGT GCCCGTGCGC ATCCGCATTG GCCTGCACTC GGGCCCGTGC 
GTGGCGGGCG TGGTGGGCCT CACCATGCCC CGGTACTGCC TGTTTGGGGA CACAGTCAAC 
ACCGCTTCGC GCATGGAGTC CACCGGGCTG CCTTACCGCA TCCACGTGAA CAGGAGCACC 
GTGCAGATCC TTTCCGCCCT AAACGAGGGC TTCCTGACTG AGGTGCGGGG CCGCACGGAG 
CTGAAGGGCA AGGGCGCAGA GGAAACCTAC TGGCTGGTGG GCAGACGCGG CTTCAACAAA 
CCCATCCCCA AACCACCGGA CCTGCAACCC GGGGCCAGCA ACCACGGCAT CAGCCTGCAC 
GAGATCCCCC CTGATCGGCG CCAGAAGCTA GAGAAGGCGA GGCCAGGCCA GTTTTCTGGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY2D ( XM_015458669.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314155.1 Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa93840
Clone ID Related Accession (Same CDS sequence)	XM_015458669.1
Accession Version	XM_015458669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	retinal guanylyl cyclase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACTCG GTTCGTGCTC GCAGAAGCCG GCAATGACCG CCTGCACCTT CCTCGCGGGT 
GGGCTTCGGG ACCCCGGACT CTGCGCCCCC ACGCGGTGGT CTCCGTCTCC GCCCGGCCTT 
CCCCCGATCC CGCCCCGGCC CCGGCTTCGG CTGAGGCCAC CGCTGCTGCT GCTGTTGCTC 
CTGCCGCGCT CCGTCCTCTC GGCCGTGTTC ACTGTGGGGG TGCTGGGTCC CTGGGCCTGC 
GACCCCATCT TCGCGCGTGC CCGCCCGGAC CTGGCCGCCC GCCTGGCCGC CTCCCGCCTG 
AACCACGCGG CTGCCCTGGA GGGCGGCCCC CGCTTCGAGG TCGCGCTGCT GCCCGAGCCG 
TGCCGGACGC CGGGCTCGCT GGGGGCCGTG TCCTCGGCGC TCACCCGCGT CTCGGGTCTA 
GTTGGGCCGG TGAACCCCGC AGCTTGCCGG CCGGCGGAAT TACTAGCCCA GGAGGCGGGG 
GTCGCTCTGG TCCCCTGGGG CTGTCCCGGG ACGCGGGCAG CGGGCACCAC GGCTCCGGTG 
GTGACGCCCG CGGCCGATGC CCTCTACGCC CTCCTGCGCG CTTTCCGCTG GGCGCACGTG 
GCCTTGGTCA CCGCCCCCCA GGACCTGTGG GTGGAGGCGG GACACGCACT TTCCACGGCG 
CTCAGGGCCA GGGGCCTGCC CGTGGCCCTG GTGACCTCCA TGGAGCCCTC CGACCTGTCT 
GGAGCCCGGG AGGCCTTGAG GAGGGTCCAA GATGGGCCCA GAGTCAGAGC AGTGATCATG 
GTAATGCACT CGGTCTTGCT GGGCGGCGAG GAGCAGCGCT GCCTGCTGGA GGCTGCAGAG 
GAACTGGGCC TGGCCGACGG TTCCCTGGTC TTCCTGCCCT TCGACACTCT CCACTACGCC 
TTGTCTCCAG GCCCTGACGC CCTGGCGGTG CTCGCCAACA GCTCACAGCT TCGCAAGGCC 
CACGATGCAG TGCTCACCCT CACCCGTCAC TGTCCCCTGG GAGGCAGCGT GCGGGACAGC 
CTGCGCAGGG CCCAAGAGCA CCGGGAGCTG CCCCTGGACC TCAATCTGCA GCAGGTATCC 
CCTCTCTTCG GCACCATCTA TGACTCAGTC TTCTTGTTGG CTGGGGGCGT GGCGCGAGCA 
CGGGTGGCCG CAGGTGGCGG CTGGGTGTCC GGAGCAGCGG TGGCCCGCCA TATCCGGGAT 
GCCCGGGTCC CTGGCTTCTG TGGGGCCCTG GGAGGAGCTG AGGAGCCCTC GTTTGTGCTA 
CTGGACACGG ATGCCACTGG GGACCAGCTG TTCGCTACAT ACGTTCTGGA CCCTACGCAG 
GGCTTCTTCC ACTCCGCTGG AACCCCAGTG CATTTTCCTA AAGGTGGACG GGGGCCTGGG 
CCTGACCCCT CGTGTTGGTT CGACCCAGAC ACCATCTGCA ACGGAGGAGT GGAACCCAGC 
GTCGTCTTTA TTGGCTTCCT CCTGGTGGTT GGGATGGGGC TGGCTGGGGC CTTCCTGGCC 
CATTATTGTA GGCACCGGCT GCTTCACATC CAAATGGTCT CTGGCCCCAA CAAGATCATC 
CTGACTCTGG ATGACATCAC CTTCCTCCAC CCGCATGGGG GCAACTCTCG AAAGGTGGCC 
CAGGGGAGTC GAACCAGTCT GGCTGCCCGC AGCATATCAG ATGTTCGCAG CATCCACAGC 
CAGCTCCCGG ATTACACCAA CATTGGCCTC TATGAGGGAG ACTGGGTTTG GCTGAAGAAA 
TTCCCAGGAG ATCGGCATAT AGCTATCCGC CCAGCAACCA AGATGGCCTT CTCCAAGATC 
CGGGAACTCC GGCATGAAAA CGTGGCCCTC TACCTGGGCC TCTTCCTGGC GGGAGGAGCT 
GGCGGCCCCG CGGCCCCCGG GGAAGGCGTG CTGGCTGTGG TCTCGGAGCA CTGCGCCCGC 
GGCTCCCTCC AAGACCTCCT CGCCCAGAGA GACATAAAGC TGGACTGGAT GTTCAAGTCT 
TCCCTCCTCC TGGACCTCAT CAAGGGAATA AGGTATCTGC ACCATCGAGG GGTGGCCCAT 
GGGCGGCTGA AGTCACGGAA CTGCGTGGTG GACGGGAGGT TCGTGCTTAA AGTCACCGAC 
CACGGTCATG GGCGACTGCT GGAGGCGCAG AGGGTGTTAC CTGAGCCACC GAGCGCGGAG 
GACCAGCTAT GGACAGCCCC GGAGCTGCTT CGAGACCCGG TCCTGGAGCG CCGGGGAACG 
CTGGCCGGCG ACGTCTTCAG TCTGGGCATC ATTATGCAGG AGGTCGTGTG CCGCAGCGCC 
CCCTACGCCA TGCTGGAGCT CACTCCCGAG GAAGTGGTGA AGAGGGTACA GAGCCCCCCT 
CCACTGTGTC GGCCCTCCGT GTCCATAGAC CAGGCGCCCA TGGAGTGCAT CCAGCTGATG 
AAACAGTGCT GGGCAGAGCA GCCAGAACTT CGGCCCTCCA TGGATCGCAC CTTTGAACTG 
TTCAAGAGCA TCAACAAGGG CCGGAAGATG AACATCATCG ACTCTATGCT GCGGATGCTG 
GAACAGTACT CCAGTAACCT GGAGGACCTG ATCCGGGAGC GCACAGAGGA GCTGGAGCTG 
GAAAAGCAGA AGACAGACCG GCTGCTCACA CAGATGCTGC CTCCGTCTGT GGCAGAGGCG 
CTGAAGATGG GGACACCTGT GGAGCCCGAG TATTTCGAAG AGGTGACACT GTACTTCAGT 
GACATTGTGG GTTTCACCAC CATCTCAGCC ATGAGTGAGC CCATCGAAGT GGTAGACCTG 
CTCAACGACC TCTACACACT CTTTGATGCC ATCATCGGTT CCCATGATGT CTACAAGGTG 
GAGACAATTG GGGATGCCTA CATGGTGGCC TCCGGCCTGC CCCAGAGGAA CGGGCACCGG 
CACGCGGCAG AGATCGCCAA TATGGCTCTG GACATCCTCA GCGCCGTGGG CACCTTCCGC 
ATGCGCCACA TGCCGGAAGT GCCCGTGCGC ATCCGCATTG GCCTGCACTC GGGCCCGTGC 
GTGGCGGGCG TGGTGGGCCT CACCATGCCC CGGTACTGCC TGTTTGGGGA CACAGTCAAC 
ACCGCTTCGC GCATGGAGTC CACCGGGCTG CGAGCACCGT GCAGATCCTT TCCGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314155.1
CDS	1123..4242
Translation	MLLGSCSQKPAMTACTFLAGGLRDPGLCAPTRWSPSPPGLPPIPPRPRLRLRPPLLLLLLLPRSVLSAVFTVGVLGPWACDPIFARARPDLAARLAASRLNHAAALEGGPRFEVALLPEPCRTPGSLGAVSSALTRVSGLVGPVNPAACRPAELLAQEAGVALVPWGCPGTRAAGTTAPVVTPAADALYALLRAFRWAHVALVTAPQDLWVEAGHALSTALRARGLPVALVTSMEPSDLSGAREALRRVQDGPRVRAVIMVMHSVLLGGEEQRCLLEAAEELGLADGSLVFLPFDTLHYALSPGPDALAVLANSSQLRKAHDAVLTLTRHCPLGGSVRDSLRRAQEHRELPLDLNLQQVSPLFGTIYDSVFLLAGGVARARVAAGGGWVSGAAVARHIRDARVPGFCGALGGAEEPSFVLLDTDATGDQLFATYVLDPTQGFFHSAGTPVHFPKGGRGPGPDPSCWFDPDTICNGGVEPSVVFIGFLLVVGMGLAGAFLAHYCRHRLLHIQMVSGPNKIILTLDDITFLHPHGGNSRKVAQGSRTSLAARSISDVRSIHSQLPDYTNIGLYEGDWVWLKKFPGDRHIAIRPATKMAFSKIRELRHENVALYLGLFLAGGAGGPAAPGEGVLAVVSEHCARGSLQDLLAQRDIKLDWMFKSSLLLDLIKGIRYLHHRGVAHGRLKSRNCVVDGRFVLKVTDHGHGRLLEAQRVLPEPPSAEDQLWTAPELLRDPVLERRGTLAGDVFSLGIIMQEVVCRSAPYAMLELTPEEVVKRVQSPPPLCRPSVSIDQAPMECIQLMKQCWAEQPELRPSMDRTFELFKSINKGRKMNIIDSMLRMLEQYSSNLEDLIRERTEELELEKQKTDRLLTQMLPPSVAEALKMGTPVEPEYFEEVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPQRNGHRHAAEIANMALDILSAVGTFRMRHMPEVPVRIRIGLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLRAPCRSFPP

Target ORF information:

RefSeq Version	XM_015458669.1
Organism	Bos taurus(cattle)
Definition	Bos taurus guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458669.1

ORF Insert Sequence:

ATGCTACTCG GTTCGTGCTC GCAGAAGCCG GCAATGACCG CCTGCACCTT CCTCGCGGGT 
GGGCTTCGGG ACCCCGGACT CTGCGCCCCC ACGCGGTGGT CTCCGTCTCC GCCCGGCCTT 
CCCCCGATCC CGCCCCGGCC CCGGCTTCGG CTGAGGCCAC CGCTGCTGCT GCTGTTGCTC 
CTGCCGCGCT CCGTCCTCTC GGCCGTGTTC ACTGTGGGGG TGCTGGGTCC CTGGGCCTGC 
GACCCCATCT TCGCGCGTGC CCGCCCGGAC CTGGCCGCCC GCCTGGCCGC CTCCCGCCTG 
AACCACGCGG CTGCCCTGGA GGGCGGCCCC CGCTTCGAGG TCGCGCTGCT GCCCGAGCCG 
TGCCGGACGC CGGGCTCGCT GGGGGCCGTG TCCTCGGCGC TCACCCGCGT CTCGGGTCTA 
GTTGGGCCGG TGAACCCCGC AGCTTGCCGG CCGGCGGAAT TACTAGCCCA GGAGGCGGGG 
GTCGCTCTGG TCCCCTGGGG CTGTCCCGGG ACGCGGGCAG CGGGCACCAC GGCTCCGGTG 
GTGACGCCCG CGGCCGATGC CCTCTACGCC CTCCTGCGCG CTTTCCGCTG GGCGCACGTG 
GCCTTGGTCA CCGCCCCCCA GGACCTGTGG GTGGAGGCGG GACACGCACT TTCCACGGCG 
CTCAGGGCCA GGGGCCTGCC CGTGGCCCTG GTGACCTCCA TGGAGCCCTC CGACCTGTCT 
GGAGCCCGGG AGGCCTTGAG GAGGGTCCAA GATGGGCCCA GAGTCAGAGC AGTGATCATG 
GTAATGCACT CGGTCTTGCT GGGCGGCGAG GAGCAGCGCT GCCTGCTGGA GGCTGCAGAG 
GAACTGGGCC TGGCCGACGG TTCCCTGGTC TTCCTGCCCT TCGACACTCT CCACTACGCC 
TTGTCTCCAG GCCCTGACGC CCTGGCGGTG CTCGCCAACA GCTCACAGCT TCGCAAGGCC 
CACGATGCAG TGCTCACCCT CACCCGTCAC TGTCCCCTGG GAGGCAGCGT GCGGGACAGC 
CTGCGCAGGG CCCAAGAGCA CCGGGAGCTG CCCCTGGACC TCAATCTGCA GCAGGTATCC 
CCTCTCTTCG GCACCATCTA TGACTCAGTC TTCTTGTTGG CTGGGGGCGT GGCGCGAGCA 
CGGGTGGCCG CAGGTGGCGG CTGGGTGTCC GGAGCAGCGG TGGCCCGCCA TATCCGGGAT 
GCCCGGGTCC CTGGCTTCTG TGGGGCCCTG GGAGGAGCTG AGGAGCCCTC GTTTGTGCTA 
CTGGACACGG ATGCCACTGG GGACCAGCTG TTCGCTACAT ACGTTCTGGA CCCTACGCAG 
GGCTTCTTCC ACTCCGCTGG AACCCCAGTG CATTTTCCTA AAGGTGGACG GGGGCCTGGG 
CCTGACCCCT CGTGTTGGTT CGACCCAGAC ACCATCTGCA ACGGAGGAGT GGAACCCAGC 
GTCGTCTTTA TTGGCTTCCT CCTGGTGGTT GGGATGGGGC TGGCTGGGGC CTTCCTGGCC 
CATTATTGTA GGCACCGGCT GCTTCACATC CAAATGGTCT CTGGCCCCAA CAAGATCATC 
CTGACTCTGG ATGACATCAC CTTCCTCCAC CCGCATGGGG GCAACTCTCG AAAGGTGGCC 
CAGGGGAGTC GAACCAGTCT GGCTGCCCGC AGCATATCAG ATGTTCGCAG CATCCACAGC 
CAGCTCCCGG ATTACACCAA CATTGGCCTC TATGAGGGAG ACTGGGTTTG GCTGAAGAAA 
TTCCCAGGAG ATCGGCATAT AGCTATCCGC CCAGCAACCA AGATGGCCTT CTCCAAGATC 
CGGGAACTCC GGCATGAAAA CGTGGCCCTC TACCTGGGCC TCTTCCTGGC GGGAGGAGCT 
GGCGGCCCCG CGGCCCCCGG GGAAGGCGTG CTGGCTGTGG TCTCGGAGCA CTGCGCCCGC 
GGCTCCCTCC AAGACCTCCT CGCCCAGAGA GACATAAAGC TGGACTGGAT GTTCAAGTCT 
TCCCTCCTCC TGGACCTCAT CAAGGGAATA AGGTATCTGC ACCATCGAGG GGTGGCCCAT 
GGGCGGCTGA AGTCACGGAA CTGCGTGGTG GACGGGAGGT TCGTGCTTAA AGTCACCGAC 
CACGGTCATG GGCGACTGCT GGAGGCGCAG AGGGTGTTAC CTGAGCCACC GAGCGCGGAG 
GACCAGCTAT GGACAGCCCC GGAGCTGCTT CGAGACCCGG TCCTGGAGCG CCGGGGAACG 
CTGGCCGGCG ACGTCTTCAG TCTGGGCATC ATTATGCAGG AGGTCGTGTG CCGCAGCGCC 
CCCTACGCCA TGCTGGAGCT CACTCCCGAG GAAGTGGTGA AGAGGGTACA GAGCCCCCCT 
CCACTGTGTC GGCCCTCCGT GTCCATAGAC CAGGCGCCCA TGGAGTGCAT CCAGCTGATG 
AAACAGTGCT GGGCAGAGCA GCCAGAACTT CGGCCCTCCA TGGATCGCAC CTTTGAACTG 
TTCAAGAGCA TCAACAAGGG CCGGAAGATG AACATCATCG ACTCTATGCT GCGGATGCTG 
GAACAGTACT CCAGTAACCT GGAGGACCTG ATCCGGGAGC GCACAGAGGA GCTGGAGCTG 
GAAAAGCAGA AGACAGACCG GCTGCTCACA CAGATGCTGC CTCCGTCTGT GGCAGAGGCG 
CTGAAGATGG GGACACCTGT GGAGCCCGAG TATTTCGAAG AGGTGACACT GTACTTCAGT 
GACATTGTGG GTTTCACCAC CATCTCAGCC ATGAGTGAGC CCATCGAAGT GGTAGACCTG 
CTCAACGACC TCTACACACT CTTTGATGCC ATCATCGGTT CCCATGATGT CTACAAGGTG 
GAGACAATTG GGGATGCCTA CATGGTGGCC TCCGGCCTGC CCCAGAGGAA CGGGCACCGG 
CACGCGGCAG AGATCGCCAA TATGGCTCTG GACATCCTCA GCGCCGTGGG CACCTTCCGC 
ATGCGCCACA TGCCGGAAGT GCCCGTGCGC ATCCGCATTG GCCTGCACTC GGGCCCGTGC 
GTGGCGGGCG TGGTGGGCCT CACCATGCCC CGGTACTGCC TGTTTGGGGA CACAGTCAAC 
ACCGCTTCGC GCATGGAGTC CACCGGGCTG CGAGCACCGT GCAGATCCTT TCCGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bicarbonate Modulates Photoreceptor Guanylate Cyclase (ROS-GC) Catalytic Activity.
The Journal of biological chemistry290(17)11052-60(2015 Apr)
Duda T,Wen XH,Isayama T,Sharma RK,Makino CL

Rod outer segment membrane guanylate cyclase type 1 (ROS-GC1) calcium-modulated transduction system in the sperm.
Fertility and sterility93(3)904-12(2010 Feb)
Jankowska A,Burczy?ska B,Duda T,Warchol JB

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The calcium-sensor guanylate cyclase activating protein type 2 specific site in rod outer segment membrane guanylate cyclase type 1.
Biochemistry44(19)7336-45(2005 May)
Duda T,Fik-Rymarkiewicz E,Venkataraman V,Krishnan R,Koch KW,Sharma RK

Guanylyl cyclase-activating proteins (GCAPs) are Ca2+/Mg2+ sensors: implications for photoreceptor guanylyl cyclase (RetGC) regulation in mammalian photoreceptors.
The Journal of biological chemistry279(17)16903-6(2004 Apr)
Peshenko IV,Dizhoor AM

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GUCY2D cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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