GUCY2D cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GUCY2D
Entrez Gene ID	3000
Full Name	guanylate cyclase 2D, retinal
Synonyms	CACD1,CORD5,CORD6,CYGD,GUC1A4,GUC2D,LCA,LCA1,RCD2,RETGC-1,ROS-GC1,ROSGC,retGC
General protein information	Preferred Names guanylate cyclase 2D, retinal Names retinal guanylyl cyclase 1 ROS-GC cone rod dystrophy 6 guanylate cyclase 2D, membrane (retina-specific) retinal guanylate cyclase 1 rod outer segment membrane guanylate cyclase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Summary	This gene encodes a retina-specific guanylate cyclase, which is a member of the membrane guanylyl cyclase family. Like other membrane guanylyl cyclases, this enzyme has a hydrophobic amino-terminal signal sequence followed by a large extracellular domain, a single membrane spanning domain, a kinase homology domain, and a guanylyl cyclase catalytic domain. In contrast to other membrane guanylyl cyclases, this enzyme is not activated by natriuretic peptides. Mutations in this gene result in Leber congenital amaurosis and cone-rod dystrophy-6 diseases. [provided by RefSeq, Dec 2008].
Disorder MIM:	600179
Disorder Html:	Leber congenital amaurosis 1, 204000 (3); Cone-rod dystrophy 6, 601777 (3); ?Central areolar choroidal dystrophy 1, 215500 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000180.4	NP_000171.1	retinal guanylyl cyclase 1
XM_011523816.1	XP_011522118.1	retinal guanylyl cyclase 1 isoform X1
NM_000180.3	NP_000171.1	retinal guanylyl cyclase 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gucy2e	NP_077356.1
Xenopus tropicalis (tropical clawed frog)	gucy2d	XP_002942678.2
	GUCY2D	XP_001111670.1
Danio rerio (zebrafish)	gc3	NP_571941.1
Bos taurus (cattle)	GUCY2D	NP_776973.2
Homo sapiens (human)	GUCY2D	NP_000171.1
Pan troglodytes (chimpanzee)	GUCY2D	XP_003315414.1
Canis lupus familiaris (dog)	GUCY2D	NP_001003207.1
Mus musculus (house mouse)	Gucy2e	NP_032218.2

Gene Ontology

The following GUCY2D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GUCY2D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17822	NM_000180.4 Latest version!	Homo sapiens guanylate cyclase 2D, retinal (GUCY2D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu17822	XM_011523816.1 Latest version!	Homo sapiens guanylate cyclase 2D, retinal (GUCY2D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
View Old Accession Versions >>
OHu17822	NM_000180.3	Homo sapiens guanylate cyclase 2D, retinal (GUCY2D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GUCY2D ( NM_000180.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000171.1 Homo sapiens guanylate cyclase 2D, retinal (GUCY2D), mRNA.

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CloneID	OHu17822
Clone ID Related Accession (Same CDS sequence)	NM_000180.4 , XM_011523816.1 , NM_000180.3
Accession Version	NM_000180.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	retinal guanylyl cyclase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104581.22. This sequence is a reference standard in the RefSeqGene project. On Aug 1, 2019 this sequence version replaced NM_000180.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M92432.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCGCCT GCGCCCGCCG AGCGGGTGGG CTTCCGGACC CCGGGCTCTG CGGTCCCGCG 
TGGTGGGCTC CGTCCCTGCC CCGCCTCCCC CGGGCCCTGC CCCGGCTCCC GCTCCTGCTG 
CTCCTGCTTC TGCTGCAGCC CCCCGCCCTC TCCGCCGTGT TCACGGTGGG GGTCCTGGGC 
CCCTGGGCTT GCGACCCCAT CTTCTCTCGG GCTCGCCCGG ACCTGGCCGC CCGCCTGGCC 
GCCGCCCGCC TGAACCGCGA CCCCGGCCTG GCAGGCGGTC CCCGCTTCGA GGTAGCGCTG 
CTGCCCGAGC CTTGCCGGAC GCCGGGCTCG CTGGGGGCCG TGTCCTCCGC GCTGGCCCGC 
GTGTCGGGCC TCGTGGGTCC GGTGAACCCT GCGGCCTGCC GGCCAGCCGA GCTGCTCGCC 
GAAGAAGCCG GGATCGCGCT GGTGCCCTGG GGCTGCCCCT GGACGCAGGC GGAGGGCACC 
ACGGCCCCTG CCGTGACCCC CGCCGCGGAT GCCCTCTACG CCCTGCTTCG CGCATTCGGC 
TGGGCGCGCG TGGCCCTGGT CACCGCCCCC CAGGACCTGT GGGTGGAGGC GGGACGCTCA 
CTGTCCACGG CACTCAGGGC CCGGGGCCTG CCTGTCGCCT CCGTGACTTC CATGGAGCCC 
TTGGACCTGT CTGGAGCCCG GGAGGCCCTG AGGAAGGTTC GGGACGGGCC CAGGGTCACA 
GCAGTGATCA TGGTGATGCA CTCGGTGCTG CTGGGTGGCG AGGAGCAGCG CTACCTCCTG 
GAGGCCGCAG AGGAGCTGGG CCTGACCGAT GGCTCCCTGG TCTTCCTGCC CTTCGACACG 
ATCCACTACG CCTTGTCCCC AGGCCCGGAG GCCTTGGCCG CACTCGCCAA CAGCTCCCAG 
CTTCGCAGGG CCCACGATGC CGTGCTCACC CTCACGCGCC ACTGTCCCTC TGAAGGCAGC 
GTGCTGGACA GCCTGCGCAG GGCTCAAGAG CGCCGCGAGC TGCCCTCTGA CCTCAATCTG 
CAGCAGGTCT CCCCACTCTT TGGCACCATC TATGACGCGG TCTTCTTGCT GGCAAGGGGC 
GTGGCAGAAG CGCGGGCTGC CGCAGGTGGC AGATGGGTGT CCGGAGCAGC TGTGGCCCGC 
CACATCCGGG ATGCGCAGGT CCCTGGCTTC TGCGGGGACC TAGGAGGAGA CGAGGAGCCC 
CCATTCGTGC TGCTAGACAC GGACGCGGCG GGAGACCGGC TTTTTGCCAC ATACATGCTG 
GATCCTGCCC GGGGCTCCTT CCTCTCCGCC GGTACCCGGA TGCACTTCCC GCGTGGGGGA 
TCAGCACCCG GACCTGACCC CTCGTGCTGG TTCGATCCAA ACAACATCTG CGGTGGAGGA 
CTGGAGCCGG GCCTCGTCTT TCTTGGCTTC CTCCTGGTGG TTGGGATGGG GCTGGCTGGG 
GCCTTCCTGG CCCATTATGT GAGGCACCGG CTACTTCACA TGCAAATGGT CTCCGGCCCC 
AACAAGATCA TCCTGACCGT GGACGACATC ACCTTTCTCC ACCCACATGG GGGCACCTCT 
CGAAAGGTGG CCCAGGGGAG TCGATCAAGT CTGGGTGCCC GCAGCATGTC AGACATTCGC 
AGCGGCCCCA GCCAACACTT GGACAGCCCC AACATTGGTG TCTATGAGGG AGACAGGGTT 
TGGCTGAAGA AATTCCCAGG GGATCAGCAC ATAGCTATCC GCCCAGCAAC CAAGACGGCC 
TTCTCCAAGC TCCAGGAGCT CCGGCATGAG AACGTGGCCC TCTACCTGGG GCTTTTCCTG 
GCTCGGGGAG CAGAAGGCCC TGCGGCCCTC TGGGAGGGCA ACCTGGCTGT GGTCTCAGAG 
CACTGCACGC GGGGCTCTCT TCAGGACCTC CTCGCTCAGA GAGAAATAAA GCTGGACTGG 
ATGTTCAAGT CCTCCCTCCT GCTGGACCTT ATCAAGGGAA TAAGGTATCT GCACCATCGA 
GGCGTGGCTC ATGGGCGGCT GAAGTCACGG AACTGCATAG TGGATGGCAG ATTCGTACTC 
AAGATCACTG ACCACGGCCA CGGGAGACTG CTGGAAGCAC AGAAGGTGCT ACCGGAGCCT 
CCCAGAGCGG AGGACCAGCT GTGGACAGCC CCGGAGCTGC TTAGGGACCC AGCCCTGGAG 
CGCCGGGGAA CGCTGGCCGG CGACGTCTTT AGCTTGGCCA TCATCATGCA AGAAGTAGTG 
TGCCGCAGTG CCCCTTATGC CATGCTGGAG CTCACTCCCG AGGAAGTGGT GCAGAGGGTG 
CGGAGCCCCC CTCCACTGTG TCGGCCCTTG GTGTCCATGG ACCAGGCACC TGTCGAGTGT 
ATCCTCCTGA TGAAGCAGTG CTGGGCAGAG CAGCCGGAAC TTCGGCCCTC CATGGACCAC 
ACCTTCGACC TGTTCAAGAA CATCAACAAG GGCCGGAAGA CGAACATCAT TGACTCGATG 
CTTCGGATGC TGGAGCAGTA CTCTAGTAAC CTGGAGGATC TGATCCGGGA GCGCACGGAG 
GAGCTGGAGC TGGAAAAGCA GAAGACAGAC CGGCTGCTTA CACAGATGCT GCCTCCGTCT 
GTGGCTGAGG CCTTGAAGAC GGGGACACCA GTGGAGCCCG AGTACTTTGA GCAAGTGACA 
CTGTACTTTA GTGACATTGT GGGCTTCACC ACCATCTCTG CCATGAGTGA GCCCATTGAG 
GTTGTGGACC TGCTCAACGA TCTCTACACA CTCTTTGATG CCATCATTGG TTCCCACGAT 
GTCTACAAGG TGGAGACAAT AGGGGACGCC TATATGGTGG CCTCGGGGCT GCCCCAGCGG 
AATGGGCAGC GACACGCGGC AGAGATCGCC AACATGTCAC TGGACATCCT CAGTGCCGTG 
GGCACTTTCC GCATGCGCCA TATGCCTGAG GTTCCCGTGC GCATCCGCAT AGGCCTGCAC 
TCGGGTCCAT GCGTGGCAGG CGTGGTGGGC CTCACCATGC CGCGGTACTG CCTGTTTGGG 
GACACGGTCA ACACCGCCTC GCGCATGGAG TCCACCGGGC TGCCTTACCG CATCCACGTG 
AACTTGAGCA CTGTGGGGAT TCTCCGTGCT CTGGACTCGG GCTACCAGGT GGAGCTGCGA 
GGCCGCACGG AGCTGAAGGG CAAGGGCGCC GAGGACACTT TCTGGCTAGT GGGCAGACGC 
GGCTTCAACA AGCCCATCCC CAAACCGCCT GACCTGCAAC CGGGGTCCAG CAACCACGGC 
ATCAGCCTGC AGGAGATCCC ACCCGAGCGG CGACGGAAGC TGGAGAAGGC GCGGCCGGGC 
CAGTTCTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000171.1
CDS	130..3441
Translation	MTACARRAGGLPDPGLCGPAWWAPSLPRLPRALPRLPLLLLLLLLQPPALSAVFTVGVLGPWACDPIFSRARPDLAARLAAARLNRDPGLAGGPRFEVALLPEPCRTPGSLGAVSSALARVSGLVGPVNPAACRPAELLAEEAGIALVPWGCPWTQAEGTTAPAVTPAADALYALLRAFGWARVALVTAPQDLWVEAGRSLSTALRARGLPVASVTSMEPLDLSGAREALRKVRDGPRVTAVIMVMHSVLLGGEEQRYLLEAAEELGLTDGSLVFLPFDTIHYALSPGPEALAALANSSQLRRAHDAVLTLTRHCPSEGSVLDSLRRAQERRELPSDLNLQQVSPLFGTIYDAVFLLARGVAEARAAAGGRWVSGAAVARHIRDAQVPGFCGDLGGDEEPPFVLLDTDAAGDRLFATYMLDPARGSFLSAGTRMHFPRGGSAPGPDPSCWFDPNNICGGGLEPGLVFLGFLLVVGMGLAGAFLAHYVRHRLLHMQMVSGPNKIILTVDDITFLHPHGGTSRKVAQGSRSSLGARSMSDIRSGPSQHLDSPNIGVYEGDRVWLKKFPGDQHIAIRPATKTAFSKLQELRHENVALYLGLFLARGAEGPAALWEGNLAVVSEHCTRGSLQDLLAQREIKLDWMFKSSLLLDLIKGIRYLHHRGVAHGRLKSRNCIVDGRFVLKITDHGHGRLLEAQKVLPEPPRAEDQLWTAPELLRDPALERRGTLAGDVFSLAIIMQEVVCRSAPYAMLELTPEEVVQRVRSPPPLCRPLVSMDQAPVECILLMKQCWAEQPELRPSMDHTFDLFKNINKGRKTNIIDSMLRMLEQYSSNLEDLIRERTEELELEKQKTDRLLTQMLPPSVAEALKTGTPVEPEYFEQVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPQRNGQRHAAEIANMSLDILSAVGTFRMRHMPEVPVRIRIGLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVNLSTVGILRALDSGYQVELRGRTELKGKGAEDTFWLVGRRGFNKPIPKPPDLQPGSSNHGISLQEIPPERRRKLEKARPGQFS

Target ORF information:

RefSeq Version	NM_000180.4
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 2D, retinal (GUCY2D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000180.4

ORF Insert Sequence:

ATGACCGCCT GCGCCCGCCG AGCGGGTGGG CTTCCGGACC CCGGGCTCTG CGGTCCCGCG 
TGGTGGGCTC CGTCCCTGCC CCGCCTCCCC CGGGCCCTGC CCCGGCTCCC GCTCCTGCTG 
CTCCTGCTTC TGCTGCAGCC CCCCGCCCTC TCCGCCGTGT TCACGGTGGG GGTCCTGGGC 
CCCTGGGCTT GCGACCCCAT CTTCTCTCGG GCTCGCCCGG ACCTGGCCGC CCGCCTGGCC 
GCCGCCCGCC TGAACCGCGA CCCCGGCCTG GCAGGCGGTC CCCGCTTCGA GGTAGCGCTG 
CTGCCCGAGC CTTGCCGGAC GCCGGGCTCG CTGGGGGCCG TGTCCTCCGC GCTGGCCCGC 
GTGTCGGGCC TCGTGGGTCC GGTGAACCCT GCGGCCTGCC GGCCAGCCGA GCTGCTCGCC 
GAAGAAGCCG GGATCGCGCT GGTGCCCTGG GGCTGCCCCT GGACGCAGGC GGAGGGCACC 
ACGGCCCCTG CCGTGACCCC CGCCGCGGAT GCCCTCTACG CCCTGCTTCG CGCATTCGGC 
TGGGCGCGCG TGGCCCTGGT CACCGCCCCC CAGGACCTGT GGGTGGAGGC GGGACGCTCA 
CTGTCCACGG CACTCAGGGC CCGGGGCCTG CCTGTCGCCT CCGTGACTTC CATGGAGCCC 
TTGGACCTGT CTGGAGCCCG GGAGGCCCTG AGGAAGGTTC GGGACGGGCC CAGGGTCACA 
GCAGTGATCA TGGTGATGCA CTCGGTGCTG CTGGGTGGCG AGGAGCAGCG CTACCTCCTG 
GAGGCCGCAG AGGAGCTGGG CCTGACCGAT GGCTCCCTGG TCTTCCTGCC CTTCGACACG 
ATCCACTACG CCTTGTCCCC AGGCCCGGAG GCCTTGGCCG CACTCGCCAA CAGCTCCCAG 
CTTCGCAGGG CCCACGATGC CGTGCTCACC CTCACGCGCC ACTGTCCCTC TGAAGGCAGC 
GTGCTGGACA GCCTGCGCAG GGCTCAAGAG CGCCGCGAGC TGCCCTCTGA CCTCAATCTG 
CAGCAGGTCT CCCCACTCTT TGGCACCATC TATGACGCGG TCTTCTTGCT GGCAAGGGGC 
GTGGCAGAAG CGCGGGCTGC CGCAGGTGGC AGATGGGTGT CCGGAGCAGC TGTGGCCCGC 
CACATCCGGG ATGCGCAGGT CCCTGGCTTC TGCGGGGACC TAGGAGGAGA CGAGGAGCCC 
CCATTCGTGC TGCTAGACAC GGACGCGGCG GGAGACCGGC TTTTTGCCAC ATACATGCTG 
GATCCTGCCC GGGGCTCCTT CCTCTCCGCC GGTACCCGGA TGCACTTCCC GCGTGGGGGA 
TCAGCACCCG GACCTGACCC CTCGTGCTGG TTCGATCCAA ACAACATCTG CGGTGGAGGA 
CTGGAGCCGG GCCTCGTCTT TCTTGGCTTC CTCCTGGTGG TTGGGATGGG GCTGGCTGGG 
GCCTTCCTGG CCCATTATGT GAGGCACCGG CTACTTCACA TGCAAATGGT CTCCGGCCCC 
AACAAGATCA TCCTGACCGT GGACGACATC ACCTTTCTCC ACCCACATGG GGGCACCTCT 
CGAAAGGTGG CCCAGGGGAG TCGATCAAGT CTGGGTGCCC GCAGCATGTC AGACATTCGC 
AGCGGCCCCA GCCAACACTT GGACAGCCCC AACATTGGTG TCTATGAGGG AGACAGGGTT 
TGGCTGAAGA AATTCCCAGG GGATCAGCAC ATAGCTATCC GCCCAGCAAC CAAGACGGCC 
TTCTCCAAGC TCCAGGAGCT CCGGCATGAG AACGTGGCCC TCTACCTGGG GCTTTTCCTG 
GCTCGGGGAG CAGAAGGCCC TGCGGCCCTC TGGGAGGGCA ACCTGGCTGT GGTCTCAGAG 
CACTGCACGC GGGGCTCTCT TCAGGACCTC CTCGCTCAGA GAGAAATAAA GCTGGACTGG 
ATGTTCAAGT CCTCCCTCCT GCTGGACCTT ATCAAGGGAA TAAGGTATCT GCACCATCGA 
GGCGTGGCTC ATGGGCGGCT GAAGTCACGG AACTGCATAG TGGATGGCAG ATTCGTACTC 
AAGATCACTG ACCACGGCCA CGGGAGACTG CTGGAAGCAC AGAAGGTGCT ACCGGAGCCT 
CCCAGAGCGG AGGACCAGCT GTGGACAGCC CCGGAGCTGC TTAGGGACCC AGCCCTGGAG 
CGCCGGGGAA CGCTGGCCGG CGACGTCTTT AGCTTGGCCA TCATCATGCA AGAAGTAGTG 
TGCCGCAGTG CCCCTTATGC CATGCTGGAG CTCACTCCCG AGGAAGTGGT GCAGAGGGTG 
CGGAGCCCCC CTCCACTGTG TCGGCCCTTG GTGTCCATGG ACCAGGCACC TGTCGAGTGT 
ATCCTCCTGA TGAAGCAGTG CTGGGCAGAG CAGCCGGAAC TTCGGCCCTC CATGGACCAC 
ACCTTCGACC TGTTCAAGAA CATCAACAAG GGCCGGAAGA CGAACATCAT TGACTCGATG 
CTTCGGATGC TGGAGCAGTA CTCTAGTAAC CTGGAGGATC TGATCCGGGA GCGCACGGAG 
GAGCTGGAGC TGGAAAAGCA GAAGACAGAC CGGCTGCTTA CACAGATGCT GCCTCCGTCT 
GTGGCTGAGG CCTTGAAGAC GGGGACACCA GTGGAGCCCG AGTACTTTGA GCAAGTGACA 
CTGTACTTTA GTGACATTGT GGGCTTCACC ACCATCTCTG CCATGAGTGA GCCCATTGAG 
GTTGTGGACC TGCTCAACGA TCTCTACACA CTCTTTGATG CCATCATTGG TTCCCACGAT 
GTCTACAAGG TGGAGACAAT AGGGGACGCC TATATGGTGG CCTCGGGGCT GCCCCAGCGG 
AATGGGCAGC GACACGCGGC AGAGATCGCC AACATGTCAC TGGACATCCT CAGTGCCGTG 
GGCACTTTCC GCATGCGCCA TATGCCTGAG GTTCCCGTGC GCATCCGCAT AGGCCTGCAC 
TCGGGTCCAT GCGTGGCAGG CGTGGTGGGC CTCACCATGC CGCGGTACTG CCTGTTTGGG 
GACACGGTCA ACACCGCCTC GCGCATGGAG TCCACCGGGC TGCCTTACCG CATCCACGTG 
AACTTGAGCA CTGTGGGGAT TCTCCGTGCT CTGGACTCGG GCTACCAGGT GGAGCTGCGA 
GGCCGCACGG AGCTGAAGGG CAAGGGCGCC GAGGACACTT TCTGGCTAGT GGGCAGACGC 
GGCTTCAACA AGCCCATCCC CAAACCGCCT GACCTGCAAC CGGGGTCCAG CAACCACGGC 
ATCAGCCTGC AGGAGATCCC ACCCGAGCGG CGACGGAAGC TGGAGAAGGC GCGGCCGGGC 
CAGTTCTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY2D ( NM_000180.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000171.1 Homo sapiens guanylate cyclase 2D, retinal (GUCY2D), mRNA.

Price & Availability↑

CloneID	OHu17822
Clone ID Related Accession (Same CDS sequence)	NM_000180.4 , XM_011523816.1 , NM_000180.3
Accession Version	NM_000180.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	retinal guanylyl cyclase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104581.22, M92432.1 and BM931704.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2008 this sequence version replaced NM_000180.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M92432.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END##

ATGACCGCCT GCGCCCGCCG AGCGGGTGGG CTTCCGGACC CCGGGCTCTG CGGTCCCGCG 
TGGTGGGCTC CGTCCCTGCC CCGCCTCCCC CGGGCCCTGC CCCGGCTCCC GCTCCTGCTG 
CTCCTGCTTC TGCTGCAGCC CCCCGCCCTC TCCGCCGTGT TCACGGTGGG GGTCCTGGGC 
CCCTGGGCTT GCGACCCCAT CTTCTCTCGG GCTCGCCCGG ACCTGGCCGC CCGCCTGGCC 
GCCGCCCGCC TGAACCGCGA CCCCGGCCTG GCAGGCGGTC CCCGCTTCGA GGTAGCGCTG 
CTGCCCGAGC CTTGCCGGAC GCCGGGCTCG CTGGGGGCCG TGTCCTCCGC GCTGGCCCGC 
GTGTCGGGCC TCGTGGGTCC GGTGAACCCT GCGGCCTGCC GGCCAGCCGA GCTGCTCGCC 
GAAGAAGCCG GGATCGCGCT GGTGCCCTGG GGCTGCCCCT GGACGCAGGC GGAGGGCACC 
ACGGCCCCTG CCGTGACCCC CGCCGCGGAT GCCCTCTACG CCCTGCTTCG CGCATTCGGC 
TGGGCGCGCG TGGCCCTGGT CACCGCCCCC CAGGACCTGT GGGTGGAGGC GGGACGCTCA 
CTGTCCACGG CACTCAGGGC CCGGGGCCTG CCTGTCGCCT CCGTGACTTC CATGGAGCCC 
TTGGACCTGT CTGGAGCCCG GGAGGCCCTG AGGAAGGTTC GGGACGGGCC CAGGGTCACA 
GCAGTGATCA TGGTGATGCA CTCGGTGCTG CTGGGTGGCG AGGAGCAGCG CTACCTCCTG 
GAGGCCGCAG AGGAGCTGGG CCTGACCGAT GGCTCCCTGG TCTTCCTGCC CTTCGACACG 
ATCCACTACG CCTTGTCCCC AGGCCCGGAG GCCTTGGCCG CACTCGCCAA CAGCTCCCAG 
CTTCGCAGGG CCCACGATGC CGTGCTCACC CTCACGCGCC ACTGTCCCTC TGAAGGCAGC 
GTGCTGGACA GCCTGCGCAG GGCTCAAGAG CGCCGCGAGC TGCCCTCTGA CCTCAATCTG 
CAGCAGGTCT CCCCACTCTT TGGCACCATC TATGACGCGG TCTTCTTGCT GGCAAGGGGC 
GTGGCAGAAG CGCGGGCTGC CGCAGGTGGC AGATGGGTGT CCGGAGCAGC TGTGGCCCGC 
CACATCCGGG ATGCGCAGGT CCCTGGCTTC TGCGGGGACC TAGGAGGAGA CGAGGAGCCC 
CCATTCGTGC TGCTAGACAC GGACGCGGCG GGAGACCGGC TTTTTGCCAC ATACATGCTG 
GATCCTGCCC GGGGCTCCTT CCTCTCCGCC GGTACCCGGA TGCACTTCCC GCGTGGGGGA 
TCAGCACCCG GACCTGACCC CTCGTGCTGG TTCGATCCAA ACAACATCTG CGGTGGAGGA 
CTGGAGCCGG GCCTCGTCTT TCTTGGCTTC CTCCTGGTGG TTGGGATGGG GCTGGCTGGG 
GCCTTCCTGG CCCATTATGT GAGGCACCGG CTACTTCACA TGCAAATGGT CTCCGGCCCC 
AACAAGATCA TCCTGACCGT GGACGACATC ACCTTTCTCC ACCCACATGG GGGCACCTCT 
CGAAAGGTGG CCCAGGGGAG TCGATCAAGT CTGGGTGCCC GCAGCATGTC AGACATTCGC 
AGCGGCCCCA GCCAACACTT GGACAGCCCC AACATTGGTG TCTATGAGGG AGACAGGGTT 
TGGCTGAAGA AATTCCCAGG GGATCAGCAC ATAGCTATCC GCCCAGCAAC CAAGACGGCC 
TTCTCCAAGC TCCAGGAGCT CCGGCATGAG AACGTGGCCC TCTACCTGGG GCTTTTCCTG 
GCTCGGGGAG CAGAAGGCCC TGCGGCCCTC TGGGAGGGCA ACCTGGCTGT GGTCTCAGAG 
CACTGCACGC GGGGCTCTCT TCAGGACCTC CTCGCTCAGA GAGAAATAAA GCTGGACTGG 
ATGTTCAAGT CCTCCCTCCT GCTGGACCTT ATCAAGGGAA TAAGGTATCT GCACCATCGA 
GGCGTGGCTC ATGGGCGGCT GAAGTCACGG AACTGCATAG TGGATGGCAG ATTCGTACTC 
AAGATCACTG ACCACGGCCA CGGGAGACTG CTGGAAGCAC AGAAGGTGCT ACCGGAGCCT 
CCCAGAGCGG AGGACCAGCT GTGGACAGCC CCGGAGCTGC TTAGGGACCC AGCCCTGGAG 
CGCCGGGGAA CGCTGGCCGG CGACGTCTTT AGCTTGGCCA TCATCATGCA AGAAGTAGTG 
TGCCGCAGTG CCCCTTATGC CATGCTGGAG CTCACTCCCG AGGAAGTGGT GCAGAGGGTG 
CGGAGCCCCC CTCCACTGTG TCGGCCCTTG GTGTCCATGG ACCAGGCACC TGTCGAGTGT 
ATCCTCCTGA TGAAGCAGTG CTGGGCAGAG CAGCCGGAAC TTCGGCCCTC CATGGACCAC 
ACCTTCGACC TGTTCAAGAA CATCAACAAG GGCCGGAAGA CGAACATCAT TGACTCGATG 
CTTCGGATGC TGGAGCAGTA CTCTAGTAAC CTGGAGGATC TGATCCGGGA GCGCACGGAG 
GAGCTGGAGC TGGAAAAGCA GAAGACAGAC CGGCTGCTTA CACAGATGCT GCCTCCGTCT 
GTGGCTGAGG CCTTGAAGAC GGGGACACCA GTGGAGCCCG AGTACTTTGA GCAAGTGACA 
CTGTACTTTA GTGACATTGT GGGCTTCACC ACCATCTCTG CCATGAGTGA GCCCATTGAG 
GTTGTGGACC TGCTCAACGA TCTCTACACA CTCTTTGATG CCATCATTGG TTCCCACGAT 
GTCTACAAGG TGGAGACAAT AGGGGACGCC TATATGGTGG CCTCGGGGCT GCCCCAGCGG 
AATGGGCAGC GACACGCGGC AGAGATCGCC AACATGTCAC TGGACATCCT CAGTGCCGTG 
GGCACTTTCC GCATGCGCCA TATGCCTGAG GTTCCCGTGC GCATCCGCAT AGGCCTGCAC 
TCGGGTCCAT GCGTGGCAGG CGTGGTGGGC CTCACCATGC CGCGGTACTG CCTGTTTGGG 
GACACGGTCA ACACCGCCTC GCGCATGGAG TCCACCGGGC TGCCTTACCG CATCCACGTG 
AACTTGAGCA CTGTGGGGAT TCTCCGTGCT CTGGACTCGG GCTACCAGGT GGAGCTGCGA 
GGCCGCACGG AGCTGAAGGG CAAGGGCGCC GAGGACACTT TCTGGCTAGT GGGCAGACGC 
GGCTTCAACA AGCCCATCCC CAAACCGCCT GACCTGCAAC CGGGGTCCAG CAACCACGGC 
ATCAGCCTGC AGGAGATCCC ACCCGAGCGG CGACGGAAGC TGGAGAAGGC GCGGCCGGGC 
CAGTTCTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000171.1
CDS	75..3386
Translation	MTACARRAGGLPDPGLCGPAWWAPSLPRLPRALPRLPLLLLLLLLQPPALSAVFTVGVLGPWACDPIFSRARPDLAARLAAARLNRDPGLAGGPRFEVALLPEPCRTPGSLGAVSSALARVSGLVGPVNPAACRPAELLAEEAGIALVPWGCPWTQAEGTTAPAVTPAADALYALLRAFGWARVALVTAPQDLWVEAGRSLSTALRARGLPVASVTSMEPLDLSGAREALRKVRDGPRVTAVIMVMHSVLLGGEEQRYLLEAAEELGLTDGSLVFLPFDTIHYALSPGPEALAALANSSQLRRAHDAVLTLTRHCPSEGSVLDSLRRAQERRELPSDLNLQQVSPLFGTIYDAVFLLARGVAEARAAAGGRWVSGAAVARHIRDAQVPGFCGDLGGDEEPPFVLLDTDAAGDRLFATYMLDPARGSFLSAGTRMHFPRGGSAPGPDPSCWFDPNNICGGGLEPGLVFLGFLLVVGMGLAGAFLAHYVRHRLLHMQMVSGPNKIILTVDDITFLHPHGGTSRKVAQGSRSSLGARSMSDIRSGPSQHLDSPNIGVYEGDRVWLKKFPGDQHIAIRPATKTAFSKLQELRHENVALYLGLFLARGAEGPAALWEGNLAVVSEHCTRGSLQDLLAQREIKLDWMFKSSLLLDLIKGIRYLHHRGVAHGRLKSRNCIVDGRFVLKITDHGHGRLLEAQKVLPEPPRAEDQLWTAPELLRDPALERRGTLAGDVFSLAIIMQEVVCRSAPYAMLELTPEEVVQRVRSPPPLCRPLVSMDQAPVECILLMKQCWAEQPELRPSMDHTFDLFKNINKGRKTNIIDSMLRMLEQYSSNLEDLIRERTEELELEKQKTDRLLTQMLPPSVAEALKTGTPVEPEYFEQVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPQRNGQRHAAEIANMSLDILSAVGTFRMRHMPEVPVRIRIGLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVNLSTVGILRALDSGYQVELRGRTELKGKGAEDTFWLVGRRGFNKPIPKPPDLQPGSSNHGISLQEIPPERRRKLEKARPGQFS

Target ORF information:

RefSeq Version	NM_000180.3
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 2D, retinal (GUCY2D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000180.3

ORF Insert Sequence:

ATGACCGCCT GCGCCCGCCG AGCGGGTGGG CTTCCGGACC CCGGGCTCTG CGGTCCCGCG 
TGGTGGGCTC CGTCCCTGCC CCGCCTCCCC CGGGCCCTGC CCCGGCTCCC GCTCCTGCTG 
CTCCTGCTTC TGCTGCAGCC CCCCGCCCTC TCCGCCGTGT TCACGGTGGG GGTCCTGGGC 
CCCTGGGCTT GCGACCCCAT CTTCTCTCGG GCTCGCCCGG ACCTGGCCGC CCGCCTGGCC 
GCCGCCCGCC TGAACCGCGA CCCCGGCCTG GCAGGCGGTC CCCGCTTCGA GGTAGCGCTG 
CTGCCCGAGC CTTGCCGGAC GCCGGGCTCG CTGGGGGCCG TGTCCTCCGC GCTGGCCCGC 
GTGTCGGGCC TCGTGGGTCC GGTGAACCCT GCGGCCTGCC GGCCAGCCGA GCTGCTCGCC 
GAAGAAGCCG GGATCGCGCT GGTGCCCTGG GGCTGCCCCT GGACGCAGGC GGAGGGCACC 
ACGGCCCCTG CCGTGACCCC CGCCGCGGAT GCCCTCTACG CCCTGCTTCG CGCATTCGGC 
TGGGCGCGCG TGGCCCTGGT CACCGCCCCC CAGGACCTGT GGGTGGAGGC GGGACGCTCA 
CTGTCCACGG CACTCAGGGC CCGGGGCCTG CCTGTCGCCT CCGTGACTTC CATGGAGCCC 
TTGGACCTGT CTGGAGCCCG GGAGGCCCTG AGGAAGGTTC GGGACGGGCC CAGGGTCACA 
GCAGTGATCA TGGTGATGCA CTCGGTGCTG CTGGGTGGCG AGGAGCAGCG CTACCTCCTG 
GAGGCCGCAG AGGAGCTGGG CCTGACCGAT GGCTCCCTGG TCTTCCTGCC CTTCGACACG 
ATCCACTACG CCTTGTCCCC AGGCCCGGAG GCCTTGGCCG CACTCGCCAA CAGCTCCCAG 
CTTCGCAGGG CCCACGATGC CGTGCTCACC CTCACGCGCC ACTGTCCCTC TGAAGGCAGC 
GTGCTGGACA GCCTGCGCAG GGCTCAAGAG CGCCGCGAGC TGCCCTCTGA CCTCAATCTG 
CAGCAGGTCT CCCCACTCTT TGGCACCATC TATGACGCGG TCTTCTTGCT GGCAAGGGGC 
GTGGCAGAAG CGCGGGCTGC CGCAGGTGGC AGATGGGTGT CCGGAGCAGC TGTGGCCCGC 
CACATCCGGG ATGCGCAGGT CCCTGGCTTC TGCGGGGACC TAGGAGGAGA CGAGGAGCCC 
CCATTCGTGC TGCTAGACAC GGACGCGGCG GGAGACCGGC TTTTTGCCAC ATACATGCTG 
GATCCTGCCC GGGGCTCCTT CCTCTCCGCC GGTACCCGGA TGCACTTCCC GCGTGGGGGA 
TCAGCACCCG GACCTGACCC CTCGTGCTGG TTCGATCCAA ACAACATCTG CGGTGGAGGA 
CTGGAGCCGG GCCTCGTCTT TCTTGGCTTC CTCCTGGTGG TTGGGATGGG GCTGGCTGGG 
GCCTTCCTGG CCCATTATGT GAGGCACCGG CTACTTCACA TGCAAATGGT CTCCGGCCCC 
AACAAGATCA TCCTGACCGT GGACGACATC ACCTTTCTCC ACCCACATGG GGGCACCTCT 
CGAAAGGTGG CCCAGGGGAG TCGATCAAGT CTGGGTGCCC GCAGCATGTC AGACATTCGC 
AGCGGCCCCA GCCAACACTT GGACAGCCCC AACATTGGTG TCTATGAGGG AGACAGGGTT 
TGGCTGAAGA AATTCCCAGG GGATCAGCAC ATAGCTATCC GCCCAGCAAC CAAGACGGCC 
TTCTCCAAGC TCCAGGAGCT CCGGCATGAG AACGTGGCCC TCTACCTGGG GCTTTTCCTG 
GCTCGGGGAG CAGAAGGCCC TGCGGCCCTC TGGGAGGGCA ACCTGGCTGT GGTCTCAGAG 
CACTGCACGC GGGGCTCTCT TCAGGACCTC CTCGCTCAGA GAGAAATAAA GCTGGACTGG 
ATGTTCAAGT CCTCCCTCCT GCTGGACCTT ATCAAGGGAA TAAGGTATCT GCACCATCGA 
GGCGTGGCTC ATGGGCGGCT GAAGTCACGG AACTGCATAG TGGATGGCAG ATTCGTACTC 
AAGATCACTG ACCACGGCCA CGGGAGACTG CTGGAAGCAC AGAAGGTGCT ACCGGAGCCT 
CCCAGAGCGG AGGACCAGCT GTGGACAGCC CCGGAGCTGC TTAGGGACCC AGCCCTGGAG 
CGCCGGGGAA CGCTGGCCGG CGACGTCTTT AGCTTGGCCA TCATCATGCA AGAAGTAGTG 
TGCCGCAGTG CCCCTTATGC CATGCTGGAG CTCACTCCCG AGGAAGTGGT GCAGAGGGTG 
CGGAGCCCCC CTCCACTGTG TCGGCCCTTG GTGTCCATGG ACCAGGCACC TGTCGAGTGT 
ATCCTCCTGA TGAAGCAGTG CTGGGCAGAG CAGCCGGAAC TTCGGCCCTC CATGGACCAC 
ACCTTCGACC TGTTCAAGAA CATCAACAAG GGCCGGAAGA CGAACATCAT TGACTCGATG 
CTTCGGATGC TGGAGCAGTA CTCTAGTAAC CTGGAGGATC TGATCCGGGA GCGCACGGAG 
GAGCTGGAGC TGGAAAAGCA GAAGACAGAC CGGCTGCTTA CACAGATGCT GCCTCCGTCT 
GTGGCTGAGG CCTTGAAGAC GGGGACACCA GTGGAGCCCG AGTACTTTGA GCAAGTGACA 
CTGTACTTTA GTGACATTGT GGGCTTCACC ACCATCTCTG CCATGAGTGA GCCCATTGAG 
GTTGTGGACC TGCTCAACGA TCTCTACACA CTCTTTGATG CCATCATTGG TTCCCACGAT 
GTCTACAAGG TGGAGACAAT AGGGGACGCC TATATGGTGG CCTCGGGGCT GCCCCAGCGG 
AATGGGCAGC GACACGCGGC AGAGATCGCC AACATGTCAC TGGACATCCT CAGTGCCGTG 
GGCACTTTCC GCATGCGCCA TATGCCTGAG GTTCCCGTGC GCATCCGCAT AGGCCTGCAC 
TCGGGTCCAT GCGTGGCAGG CGTGGTGGGC CTCACCATGC CGCGGTACTG CCTGTTTGGG 
GACACGGTCA ACACCGCCTC GCGCATGGAG TCCACCGGGC TGCCTTACCG CATCCACGTG 
AACTTGAGCA CTGTGGGGAT TCTCCGTGCT CTGGACTCGG GCTACCAGGT GGAGCTGCGA 
GGCCGCACGG AGCTGAAGGG CAAGGGCGCC GAGGACACTT TCTGGCTAGT GGGCAGACGC 
GGCTTCAACA AGCCCATCCC CAAACCGCCT GACCTGCAAC CGGGGTCCAG CAACCACGGC 
ATCAGCCTGC AGGAGATCCC ACCCGAGCGG CGACGGAAGC TGGAGAAGGC GCGGCCGGGC 
CAGTTCTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GUCY2D ( XM_011523816.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522118.1 Homo sapiens guanylate cyclase 2D, retinal (GUCY2D), transcript variant X1, mRNA.

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CloneID	OHu17822
Clone ID Related Accession (Same CDS sequence)	NM_000180.4 , XM_011523816.1 , NM_000180.3
Accession Version	XM_011523816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	retinal guanylyl cyclase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGCCT GCGCCCGCCG AGCGGGTGGG CTTCCGGACC CCGGGCTCTG CGGTCCCGCG 
TGGTGGGCTC CGTCCCTGCC CCGCCTCCCC CGGGCCCTGC CCCGGCTCCC GCTCCTGCTG 
CTCCTGCTTC TGCTGCAGCC CCCCGCCCTC TCCGCCGTGT TCACGGTGGG GGTCCTGGGC 
CCCTGGGCTT GCGACCCCAT CTTCTCTCGG GCTCGCCCGG ACCTGGCCGC CCGCCTGGCC 
GCCGCCCGCC TGAACCGCGA CCCCGGCCTG GCAGGCGGTC CCCGCTTCGA GGTAGCGCTG 
CTGCCCGAGC CTTGCCGGAC GCCGGGCTCG CTGGGGGCCG TGTCCTCCGC GCTGGCCCGC 
GTGTCGGGCC TCGTGGGTCC GGTGAACCCT GCGGCCTGCC GGCCAGCCGA GCTGCTCGCC 
GAAGAAGCCG GGATCGCGCT GGTGCCCTGG GGCTGCCCCT GGACGCAGGC GGAGGGCACC 
ACGGCCCCTG CCGTGACCCC CGCCGCGGAT GCCCTCTACG CCCTGCTTCG CGCATTCGGC 
TGGGCGCGCG TGGCCCTGGT CACCGCCCCC CAGGACCTGT GGGTGGAGGC GGGACGCTCA 
CTGTCCACGG CACTCAGGGC CCGGGGCCTG CCTGTCGCCT CCGTGACTTC CATGGAGCCC 
TTGGACCTGT CTGGAGCCCG GGAGGCCCTG AGGAAGGTTC GGGACGGGCC CAGGGTCACA 
GCAGTGATCA TGGTGATGCA CTCGGTGCTG CTGGGTGGCG AGGAGCAGCG CTACCTCCTG 
GAGGCCGCAG AGGAGCTGGG CCTGACCGAT GGCTCCCTGG TCTTCCTGCC CTTCGACACG 
ATCCACTACG CCTTGTCCCC AGGCCCGGAG GCCTTGGCCG CACTCGCCAA CAGCTCCCAG 
CTTCGCAGGG CCCACGATGC CGTGCTCACC CTCACGCGCC ACTGTCCCTC TGAAGGCAGC 
GTGCTGGACA GCCTGCGCAG GGCTCAAGAG CGCCGCGAGC TGCCCTCTGA CCTCAATCTG 
CAGCAGGTCT CCCCACTCTT TGGCACCATC TATGACGCGG TCTTCTTGCT GGCAAGGGGC 
GTGGCAGAAG CGCGGGCTGC CGCAGGTGGC AGATGGGTGT CCGGAGCAGC TGTGGCCCGC 
CACATCCGGG ATGCGCAGGT CCCTGGCTTC TGCGGGGACC TAGGAGGAGA CGAGGAGCCC 
CCATTCGTGC TGCTAGACAC GGACGCGGCG GGAGACCGGC TTTTTGCCAC ATACATGCTG 
GATCCTGCCC GGGGCTCCTT CCTCTCCGCC GGTACCCGGA TGCACTTCCC GCGTGGGGGA 
TCAGCACCCG GACCTGACCC CTCGTGCTGG TTCGATCCAA ACAACATCTG CGGTGGAGGA 
CTGGAGCCGG GCCTCGTCTT TCTTGGCTTC CTCCTGGTGG TTGGGATGGG GCTGGCTGGG 
GCCTTCCTGG CCCATTATGT GAGGCACCGG CTACTTCACA TGCAAATGGT CTCCGGCCCC 
AACAAGATCA TCCTGACCGT GGACGACATC ACCTTTCTCC ACCCACATGG GGGCACCTCT 
CGAAAGGTGG CCCAGGGGAG TCGATCAAGT CTGGGTGCCC GCAGCATGTC AGACATTCGC 
AGCGGCCCCA GCCAACACTT GGACAGCCCC AACATTGGTG TCTATGAGGG AGACAGGGTT 
TGGCTGAAGA AATTCCCAGG GGATCAGCAC ATAGCTATCC GCCCAGCAAC CAAGACGGCC 
TTCTCCAAGC TCCAGGAGCT CCGGCATGAG AACGTGGCCC TCTACCTGGG GCTTTTCCTG 
GCTCGGGGAG CAGAAGGCCC TGCGGCCCTC TGGGAGGGCA ACCTGGCTGT GGTCTCAGAG 
CACTGCACGC GGGGCTCTCT TCAGGACCTC CTCGCTCAGA GAGAAATAAA GCTGGACTGG 
ATGTTCAAGT CCTCCCTCCT GCTGGACCTT ATCAAGGGAA TAAGGTATCT GCACCATCGA 
GGCGTGGCTC ATGGGCGGCT GAAGTCACGG AACTGCATAG TGGATGGCAG ATTCGTACTC 
AAGATCACTG ACCACGGCCA CGGGAGACTG CTGGAAGCAC AGAAGGTGCT ACCGGAGCCT 
CCCAGAGCGG AGGACCAGCT GTGGACAGCC CCGGAGCTGC TTAGGGACCC AGCCCTGGAG 
CGCCGGGGAA CGCTGGCCGG CGACGTCTTT AGCTTGGCCA TCATCATGCA AGAAGTAGTG 
TGCCGCAGTG CCCCTTATGC CATGCTGGAG CTCACTCCCG AGGAAGTGGT GCAGAGGGTG 
CGGAGCCCCC CTCCACTGTG TCGGCCCTTG GTGTCCATGG ACCAGGCACC TGTCGAGTGT 
ATCCTCCTGA TGAAGCAGTG CTGGGCAGAG CAGCCGGAAC TTCGGCCCTC CATGGACCAC 
ACCTTCGACC TGTTCAAGAA CATCAACAAG GGCCGGAAGA CGAACATCAT TGACTCGATG 
CTTCGGATGC TGGAGCAGTA CTCTAGTAAC CTGGAGGATC TGATCCGGGA GCGCACGGAG 
GAGCTGGAGC TGGAAAAGCA GAAGACAGAC CGGCTGCTTA CACAGATGCT GCCTCCGTCT 
GTGGCTGAGG CCTTGAAGAC GGGGACACCA GTGGAGCCCG AGTACTTTGA GCAAGTGACA 
CTGTACTTTA GTGACATTGT GGGCTTCACC ACCATCTCTG CCATGAGTGA GCCCATTGAG 
GTTGTGGACC TGCTCAACGA TCTCTACACA CTCTTTGATG CCATCATTGG TTCCCACGAT 
GTCTACAAGG TGGAGACAAT AGGGGACGCC TATATGGTGG CCTCGGGGCT GCCCCAGCGG 
AATGGGCAGC GACACGCGGC AGAGATCGCC AACATGTCAC TGGACATCCT CAGTGCCGTG 
GGCACTTTCC GCATGCGCCA TATGCCTGAG GTTCCCGTGC GCATCCGCAT AGGCCTGCAC 
TCGGGTCCAT GCGTGGCAGG CGTGGTGGGC CTCACCATGC CGCGGTACTG CCTGTTTGGG 
GACACGGTCA ACACCGCCTC GCGCATGGAG TCCACCGGGC TGCCTTACCG CATCCACGTG 
AACTTGAGCA CTGTGGGGAT TCTCCGTGCT CTGGACTCGG GCTACCAGGT GGAGCTGCGA 
GGCCGCACGG AGCTGAAGGG CAAGGGCGCC GAGGACACTT TCTGGCTAGT GGGCAGACGC 
GGCTTCAACA AGCCCATCCC CAAACCGCCT GACCTGCAAC CGGGGTCCAG CAACCACGGC 
ATCAGCCTGC AGGAGATCCC ACCCGAGCGG CGACGGAAGC TGGAGAAGGC GCGGCCGGGC 
CAGTTCTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522118.1
CDS	1..3312
Translation	MTACARRAGGLPDPGLCGPAWWAPSLPRLPRALPRLPLLLLLLLLQPPALSAVFTVGVLGPWACDPIFSRARPDLAARLAAARLNRDPGLAGGPRFEVALLPEPCRTPGSLGAVSSALARVSGLVGPVNPAACRPAELLAEEAGIALVPWGCPWTQAEGTTAPAVTPAADALYALLRAFGWARVALVTAPQDLWVEAGRSLSTALRARGLPVASVTSMEPLDLSGAREALRKVRDGPRVTAVIMVMHSVLLGGEEQRYLLEAAEELGLTDGSLVFLPFDTIHYALSPGPEALAALANSSQLRRAHDAVLTLTRHCPSEGSVLDSLRRAQERRELPSDLNLQQVSPLFGTIYDAVFLLARGVAEARAAAGGRWVSGAAVARHIRDAQVPGFCGDLGGDEEPPFVLLDTDAAGDRLFATYMLDPARGSFLSAGTRMHFPRGGSAPGPDPSCWFDPNNICGGGLEPGLVFLGFLLVVGMGLAGAFLAHYVRHRLLHMQMVSGPNKIILTVDDITFLHPHGGTSRKVAQGSRSSLGARSMSDIRSGPSQHLDSPNIGVYEGDRVWLKKFPGDQHIAIRPATKTAFSKLQELRHENVALYLGLFLARGAEGPAALWEGNLAVVSEHCTRGSLQDLLAQREIKLDWMFKSSLLLDLIKGIRYLHHRGVAHGRLKSRNCIVDGRFVLKITDHGHGRLLEAQKVLPEPPRAEDQLWTAPELLRDPALERRGTLAGDVFSLAIIMQEVVCRSAPYAMLELTPEEVVQRVRSPPPLCRPLVSMDQAPVECILLMKQCWAEQPELRPSMDHTFDLFKNINKGRKTNIIDSMLRMLEQYSSNLEDLIRERTEELELEKQKTDRLLTQMLPPSVAEALKTGTPVEPEYFEQVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPQRNGQRHAAEIANMSLDILSAVGTFRMRHMPEVPVRIRIGLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVNLSTVGILRALDSGYQVELRGRTELKGKGAEDTFWLVGRRGFNKPIPKPPDLQPGSSNHGISLQEIPPERRRKLEKARPGQFS

Target ORF information:

RefSeq Version	XM_011523816.1
Organism	Homo sapiens(human)
Definition	Homo sapiens guanylate cyclase 2D, retinal (GUCY2D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523816.1

ORF Insert Sequence:

ATGACCGCCT GCGCCCGCCG AGCGGGTGGG CTTCCGGACC CCGGGCTCTG CGGTCCCGCG 
TGGTGGGCTC CGTCCCTGCC CCGCCTCCCC CGGGCCCTGC CCCGGCTCCC GCTCCTGCTG 
CTCCTGCTTC TGCTGCAGCC CCCCGCCCTC TCCGCCGTGT TCACGGTGGG GGTCCTGGGC 
CCCTGGGCTT GCGACCCCAT CTTCTCTCGG GCTCGCCCGG ACCTGGCCGC CCGCCTGGCC 
GCCGCCCGCC TGAACCGCGA CCCCGGCCTG GCAGGCGGTC CCCGCTTCGA GGTAGCGCTG 
CTGCCCGAGC CTTGCCGGAC GCCGGGCTCG CTGGGGGCCG TGTCCTCCGC GCTGGCCCGC 
GTGTCGGGCC TCGTGGGTCC GGTGAACCCT GCGGCCTGCC GGCCAGCCGA GCTGCTCGCC 
GAAGAAGCCG GGATCGCGCT GGTGCCCTGG GGCTGCCCCT GGACGCAGGC GGAGGGCACC 
ACGGCCCCTG CCGTGACCCC CGCCGCGGAT GCCCTCTACG CCCTGCTTCG CGCATTCGGC 
TGGGCGCGCG TGGCCCTGGT CACCGCCCCC CAGGACCTGT GGGTGGAGGC GGGACGCTCA 
CTGTCCACGG CACTCAGGGC CCGGGGCCTG CCTGTCGCCT CCGTGACTTC CATGGAGCCC 
TTGGACCTGT CTGGAGCCCG GGAGGCCCTG AGGAAGGTTC GGGACGGGCC CAGGGTCACA 
GCAGTGATCA TGGTGATGCA CTCGGTGCTG CTGGGTGGCG AGGAGCAGCG CTACCTCCTG 
GAGGCCGCAG AGGAGCTGGG CCTGACCGAT GGCTCCCTGG TCTTCCTGCC CTTCGACACG 
ATCCACTACG CCTTGTCCCC AGGCCCGGAG GCCTTGGCCG CACTCGCCAA CAGCTCCCAG 
CTTCGCAGGG CCCACGATGC CGTGCTCACC CTCACGCGCC ACTGTCCCTC TGAAGGCAGC 
GTGCTGGACA GCCTGCGCAG GGCTCAAGAG CGCCGCGAGC TGCCCTCTGA CCTCAATCTG 
CAGCAGGTCT CCCCACTCTT TGGCACCATC TATGACGCGG TCTTCTTGCT GGCAAGGGGC 
GTGGCAGAAG CGCGGGCTGC CGCAGGTGGC AGATGGGTGT CCGGAGCAGC TGTGGCCCGC 
CACATCCGGG ATGCGCAGGT CCCTGGCTTC TGCGGGGACC TAGGAGGAGA CGAGGAGCCC 
CCATTCGTGC TGCTAGACAC GGACGCGGCG GGAGACCGGC TTTTTGCCAC ATACATGCTG 
GATCCTGCCC GGGGCTCCTT CCTCTCCGCC GGTACCCGGA TGCACTTCCC GCGTGGGGGA 
TCAGCACCCG GACCTGACCC CTCGTGCTGG TTCGATCCAA ACAACATCTG CGGTGGAGGA 
CTGGAGCCGG GCCTCGTCTT TCTTGGCTTC CTCCTGGTGG TTGGGATGGG GCTGGCTGGG 
GCCTTCCTGG CCCATTATGT GAGGCACCGG CTACTTCACA TGCAAATGGT CTCCGGCCCC 
AACAAGATCA TCCTGACCGT GGACGACATC ACCTTTCTCC ACCCACATGG GGGCACCTCT 
CGAAAGGTGG CCCAGGGGAG TCGATCAAGT CTGGGTGCCC GCAGCATGTC AGACATTCGC 
AGCGGCCCCA GCCAACACTT GGACAGCCCC AACATTGGTG TCTATGAGGG AGACAGGGTT 
TGGCTGAAGA AATTCCCAGG GGATCAGCAC ATAGCTATCC GCCCAGCAAC CAAGACGGCC 
TTCTCCAAGC TCCAGGAGCT CCGGCATGAG AACGTGGCCC TCTACCTGGG GCTTTTCCTG 
GCTCGGGGAG CAGAAGGCCC TGCGGCCCTC TGGGAGGGCA ACCTGGCTGT GGTCTCAGAG 
CACTGCACGC GGGGCTCTCT TCAGGACCTC CTCGCTCAGA GAGAAATAAA GCTGGACTGG 
ATGTTCAAGT CCTCCCTCCT GCTGGACCTT ATCAAGGGAA TAAGGTATCT GCACCATCGA 
GGCGTGGCTC ATGGGCGGCT GAAGTCACGG AACTGCATAG TGGATGGCAG ATTCGTACTC 
AAGATCACTG ACCACGGCCA CGGGAGACTG CTGGAAGCAC AGAAGGTGCT ACCGGAGCCT 
CCCAGAGCGG AGGACCAGCT GTGGACAGCC CCGGAGCTGC TTAGGGACCC AGCCCTGGAG 
CGCCGGGGAA CGCTGGCCGG CGACGTCTTT AGCTTGGCCA TCATCATGCA AGAAGTAGTG 
TGCCGCAGTG CCCCTTATGC CATGCTGGAG CTCACTCCCG AGGAAGTGGT GCAGAGGGTG 
CGGAGCCCCC CTCCACTGTG TCGGCCCTTG GTGTCCATGG ACCAGGCACC TGTCGAGTGT 
ATCCTCCTGA TGAAGCAGTG CTGGGCAGAG CAGCCGGAAC TTCGGCCCTC CATGGACCAC 
ACCTTCGACC TGTTCAAGAA CATCAACAAG GGCCGGAAGA CGAACATCAT TGACTCGATG 
CTTCGGATGC TGGAGCAGTA CTCTAGTAAC CTGGAGGATC TGATCCGGGA GCGCACGGAG 
GAGCTGGAGC TGGAAAAGCA GAAGACAGAC CGGCTGCTTA CACAGATGCT GCCTCCGTCT 
GTGGCTGAGG CCTTGAAGAC GGGGACACCA GTGGAGCCCG AGTACTTTGA GCAAGTGACA 
CTGTACTTTA GTGACATTGT GGGCTTCACC ACCATCTCTG CCATGAGTGA GCCCATTGAG 
GTTGTGGACC TGCTCAACGA TCTCTACACA CTCTTTGATG CCATCATTGG TTCCCACGAT 
GTCTACAAGG TGGAGACAAT AGGGGACGCC TATATGGTGG CCTCGGGGCT GCCCCAGCGG 
AATGGGCAGC GACACGCGGC AGAGATCGCC AACATGTCAC TGGACATCCT CAGTGCCGTG 
GGCACTTTCC GCATGCGCCA TATGCCTGAG GTTCCCGTGC GCATCCGCAT AGGCCTGCAC 
TCGGGTCCAT GCGTGGCAGG CGTGGTGGGC CTCACCATGC CGCGGTACTG CCTGTTTGGG 
GACACGGTCA ACACCGCCTC GCGCATGGAG TCCACCGGGC TGCCTTACCG CATCCACGTG 
AACTTGAGCA CTGTGGGGAT TCTCCGTGCT CTGGACTCGG GCTACCAGGT GGAGCTGCGA 
GGCCGCACGG AGCTGAAGGG CAAGGGCGCC GAGGACACTT TCTGGCTAGT GGGCAGACGC 
GGCTTCAACA AGCCCATCCC CAAACCGCCT GACCTGCAAC CGGGGTCCAG CAACCACGGC 
ATCAGCCTGC AGGAGATCCC ACCCGAGCGG CGACGGAAGC TGGAGAAGGC GCGGCCGGGC 
CAGTTCTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GUCY2D cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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