PPP1R16B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP1R16B
Entrez Gene ID	282091
Full Name	protein phosphatase 1 regulatory subunit 16B
Synonyms	TIMAP
General protein information	Preferred Names protein phosphatase 1 regulatory subunit 16B Names protein phosphatase 1 regulatory inhibitor subunit 16B CAAX box protein TIMAP TGF-beta-inhibited membrane-associated protein bTIMAP protein phosphatase 1, regulatory (inhibitor) subunit 16B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005214496.4	XP_005214553.1	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X1
XM_005214496.3	XP_005214553.1	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X1
NM_174824.2	NP_777249.1	protein phosphatase 1 regulatory inhibitor subunit 16B

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPP1R16B	NP_056383.1
Gallus gallus (chicken)	PPP1R16B	NP_001026022.1
Xenopus tropicalis (tropical clawed frog)	ppp1r16b	NP_001123752.1
Mus musculus (house mouse)	Ppp1r16b	NP_694729.1
Pan troglodytes (chimpanzee)	PPP1R16B	XP_003317035.1
Rattus norvegicus (Norway rat)	Ppp1r16b	NP_001178001.1
Danio rerio (zebrafish)	LOC799771	XP_001340092.5
Canis lupus familiaris (dog)	PPP1R16B	XP_003433312.1
Bos taurus (cattle)	PPP1R16B	NP_777249.1

Gene Ontology
Bibliography

Related articles in PubMed

TIMAP-protein phosphatase 1-complex controls endothelin-1 production via ECE-1 dephosphorylation.
Boratk? A, Ver?b Z, Petrovski G, Csortos C
The international journal of biochemistry & cell biology7311-18(2016 Apr)

Multi-directional function of the protein phosphatase 1 regulatory subunit TIMAP.
Shopik MJ, Li L, Luu HA, Obeidat M, Holmes CF, Ballermann BJ
Biochemical and biophysical research communications435(4)567-73(2013 Jun)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Phosphorylation of TIMAP by glycogen synthase kinase-3beta activates its associated protein phosphatase 1.
Li L, Kozlowski K, Wegner B, Rashid T, Yeung T, Holmes C, Ballermann BJ
The Journal of biological chemistry282(35)25960-9(2007 Aug)

TIMAP, a novel CAAX box protein regulated by TGF-beta1 and expressed in endothelial cells.
Cao W, Mattagajasingh SN, Xu H, Kim K, Fierlbeck W, Deng J, Lowenstein CJ, Ballermann BJ
American journal of physiology. Cell physiology283(1)C327-37(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TIMAP can modify protein phosphorylation independent of its function as a PP1c regulatory subunit, namely by masking phosphorylation sites of binding partners like PP1c and LAMR1.
Title: Multi-directional function of the protein phosphatase 1 regulatory subunit TIMAP.

TIMAP is a target for PKA-primed GSK-3beta-mediated phosphorylation. This phosphorylation controls TIMAP association and activity of PP1c
Title: Phosphorylation of TIMAP by glycogen synthase kinase-3beta activates its associated protein phosphatase 1.

The following PPP1R16B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R16B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa25285	XM_005214496.4 Latest version!	Bos taurus protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa43944	NM_174824.2 Latest version!	Bos taurus protein phosphatase 1 regulatory subunit 16B (PPP1R16B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OBa25285	XM_005214496.3	Bos taurus protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP1R16B ( XM_005214496.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214553.1 Bos taurus protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa25285
Clone ID Related Accession (Same CDS sequence)	XM_005214496.4 , XM_005214496.3
Accession Version	XM_005214496.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005214496.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC ACGTGGACCT GCTGACCGAA CTGCAGCTGC TGGAGAAGGT GCCCACGTTG 
GAGCGGCTGC GCGCAGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAGTG GGCGCAGTAT 
GAGCAGGACC TGCAGCATCG CAAGCGCAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGG 
CGAAAGAAGG TGTCCTTCGA GGCCAGTGTG GCCCTCCTGG AGGCCTCGCT TAGGAACGAC 
GCTGAAGAAG TGCGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAACGAAGAT 
GGACTCACCG CCCTGCACCA GTGCTGCATC GACAACTTTG AGGAAATTGT CAAGCTGCTC 
CTTTCGCACG GTGCCAACGT GAACGCCAAG GACAACGAGC TGTGGACGCC CCTGCACGCC 
GCGGCCACCT GCGGCCACAT TAACCTGGTG AAAATCCTCG TTCAGTATGG AGCCGACTTG 
CTCGCTGTCA ACTCCGATGG GAACATGCCC TATGACCTCT GTGAGGATGA GCCCACCCTG 
GATGTTATCG AGACGTGCAT GGCGTACCAG GGCATCACCC AGGAGAAAAT CAACGAGATG 
CGGGCAGCTC CCGAGCAGCA GATGATTTCA GACATTCACT GTATGATCGC AGCTGGCCAG 
GACCTTGATT GGGTGGATGC CCAGGGGGCC ACACTGCTGC ACATAGCTGG AGCCAACGGA 
TACCTGCGGG CAGCCGAGCT CCTCCTGGAT CATGGTGTAC GCGTGGATGT GAAGGACTGG 
GATGGCTGGG AGCCCCTGCA CGCCGCTGCC TTCTGGGGAC AGATGCAGAT GGCAGAGCTC 
TTGGTGTCCC ATGGGGCCAG TCTCAGTGCT AGGACGTCCA TGGACGAGAT GCCAATTGAC 
CTGTGTGAAG AGGAGGAGTT CAAAGTCCTG CTGTTGGAGC TGAAACACAA GCACGACGTG 
ATCATGAAGT CGCAGCTGAG GCATAAGTCA TCTTTGAGCA GGAGGACGTC CAGCGCGGGC 
AGCCGAGGGA AGGTGGTACG TCGAGCCAGC CTGTCCGACA GGACCAACCT GTACAGGAAG 
GAGTATGAGG GAGAGGCTAT ACTCTGGCAG CAGCGGAGTG CATCCGAGGA TCAGCGGAAC 
TCCACCTACA ACGGGGACAT CAGGGAGACC AGGACAGACC AAGAGAATAA GGACCCGAAC 
CCCAGGCTGG AGAAGCCCGT GCTGCTCTCC GAGTTTCCCA CCAAGATCCC ACACAGCGAC 
ATGGACATGC CTGTTGAGAA CGGCCTCCGG GCTCCAGTCA GCACCTACCA GTATGCGCTG 
TGTAACGGGG ACGTCTGGAA AGTGCACGAG GTGCCCGACT ACAGCATGGC CTATGGCAAC 
CCTGGCGTGG CCGATGCCAC CCCATCCTGG AGTGGCTACA AGGAACAGAG CCCCCAGACG 
CTTCTGGAGC TGAAGAGGCA GCGGGCTGCG GCCAAGCTGC TCAGCCACCC CTTCCTGAGC 
ACCCACCTGG GCAGTGGCGT GTCCAGGACA GGCGAGGGCA GCAGCGAAGG CAAGGCCCCC 
TTGATCGGAG GCAGAACTTC TCCGTACAGT AGCAACGGGA CCTCGGTGTA TTACACGGTC 
ACCAGTGGAG ACCCCCCACT CCTGAAGTTC AAGGCCCCCA TAGAGGAGAT GGAGGAGAAG 
GTCCATGGCT GCTGCCGCAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214553.1
CDS	252..1958
Translation	MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDLQHRKRKHERKRSTGGRRKKVSFEASVALLEASLRNDAEEVRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRAAPEQQMISDIHCMIAAGQDLDWVDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQQRSASEDQRNSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPHSDMDMPVENGLRAPVSTYQYALCNGDVWKVHEVPDYSMAYGNPGVADATPSWSGYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSGVSRTGEGSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	XM_005214496.4
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214496.4

ORF Insert Sequence:

ATGGCCAGCC ACGTGGACCT GCTGACCGAA CTGCAGCTGC TGGAGAAGGT GCCCACGTTG 
GAGCGGCTGC GCGCAGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAGTG GGCGCAGTAT 
GAGCAGGACC TGCAGCATCG CAAGCGCAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGG 
CGAAAGAAGG TGTCCTTCGA GGCCAGTGTG GCCCTCCTGG AGGCCTCGCT TAGGAACGAC 
GCTGAAGAAG TGCGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAACGAAGAT 
GGACTCACCG CCCTGCACCA GTGCTGCATC GACAACTTTG AGGAAATTGT CAAGCTGCTC 
CTTTCGCACG GTGCCAACGT GAACGCCAAG GACAACGAGC TGTGGACGCC CCTGCACGCC 
GCGGCCACCT GCGGCCACAT TAACCTGGTG AAAATCCTCG TTCAGTATGG AGCCGACTTG 
CTCGCTGTCA ACTCCGATGG GAACATGCCC TATGACCTCT GTGAGGATGA GCCCACCCTG 
GATGTTATCG AGACGTGCAT GGCGTACCAG GGCATCACCC AGGAGAAAAT CAACGAGATG 
CGGGCAGCTC CCGAGCAGCA GATGATTTCA GACATTCACT GTATGATCGC AGCTGGCCAG 
GACCTTGATT GGGTGGATGC CCAGGGGGCC ACACTGCTGC ACATAGCTGG AGCCAACGGA 
TACCTGCGGG CAGCCGAGCT CCTCCTGGAT CATGGTGTAC GCGTGGATGT GAAGGACTGG 
GATGGCTGGG AGCCCCTGCA CGCCGCTGCC TTCTGGGGAC AGATGCAGAT GGCAGAGCTC 
TTGGTGTCCC ATGGGGCCAG TCTCAGTGCT AGGACGTCCA TGGACGAGAT GCCAATTGAC 
CTGTGTGAAG AGGAGGAGTT CAAAGTCCTG CTGTTGGAGC TGAAACACAA GCACGACGTG 
ATCATGAAGT CGCAGCTGAG GCATAAGTCA TCTTTGAGCA GGAGGACGTC CAGCGCGGGC 
AGCCGAGGGA AGGTGGTACG TCGAGCCAGC CTGTCCGACA GGACCAACCT GTACAGGAAG 
GAGTATGAGG GAGAGGCTAT ACTCTGGCAG CAGCGGAGTG CATCCGAGGA TCAGCGGAAC 
TCCACCTACA ACGGGGACAT CAGGGAGACC AGGACAGACC AAGAGAATAA GGACCCGAAC 
CCCAGGCTGG AGAAGCCCGT GCTGCTCTCC GAGTTTCCCA CCAAGATCCC ACACAGCGAC 
ATGGACATGC CTGTTGAGAA CGGCCTCCGG GCTCCAGTCA GCACCTACCA GTATGCGCTG 
TGTAACGGGG ACGTCTGGAA AGTGCACGAG GTGCCCGACT ACAGCATGGC CTATGGCAAC 
CCTGGCGTGG CCGATGCCAC CCCATCCTGG AGTGGCTACA AGGAACAGAG CCCCCAGACG 
CTTCTGGAGC TGAAGAGGCA GCGGGCTGCG GCCAAGCTGC TCAGCCACCC CTTCCTGAGC 
ACCCACCTGG GCAGTGGCGT GTCCAGGACA GGCGAGGGCA GCAGCGAAGG CAAGGCCCCC 
TTGATCGGAG GCAGAACTTC TCCGTACAGT AGCAACGGGA CCTCGGTGTA TTACACGGTC 
ACCAGTGGAG ACCCCCCACT CCTGAAGTTC AAGGCCCCCA TAGAGGAGAT GGAGGAGAAG 
GTCCATGGCT GCTGCCGCAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R16B ( XM_005214496.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214553.1 Bos taurus protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa25285
Clone ID Related Accession (Same CDS sequence)	XM_005214496.4 , XM_005214496.3
Accession Version	XM_005214496.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC ACGTGGACCT GCTGACCGAA CTGCAGCTGC TGGAGAAGGT GCCCACGTTG 
GAGCGGCTGC GCGCAGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAGTG GGCGCAGTAT 
GAGCAGGACC TGCAGCATCG CAAGCGCAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGG 
CGAAAGAAGG TGTCCTTCGA GGCCAGTGTG GCCCTCCTGG AGGCCTCGCT TAGGAACGAC 
GCTGAAGAAG TGCGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAACGAAGAT 
GGACTCACCG CCCTGCACCA GTGCTGCATC GACAACTTTG AGGAAATTGT CAAGCTGCTC 
CTTTCGCACG GTGCCAACGT GAACGCCAAG GACAACGAGC TGTGGACGCC CCTGCACGCC 
GCGGCCACCT GCGGCCACAT TAACCTGGTG AAAATCCTCG TTCAGTATGG AGCCGACTTG 
CTCGCTGTCA ACTCCGATGG GAACATGCCC TATGACCTCT GTGAGGATGA GCCCACCCTG 
GATGTTATCG AGACGTGCAT GGCGTACCAG GGCATCACCC AGGAGAAAAT CAACGAGATG 
CGGGCAGCTC CCGAGCAGCA GATGATTTCA GACATTCACT GTATGATCGC AGCTGGCCAG 
GACCTTGATT GGGTGGATGC CCAGGGGGCC ACACTGCTGC ACATAGCTGG AGCCAACGGA 
TACCTGCGGG CAGCCGAGCT CCTCCTGGAT CATGGTGTAC GCGTGGATGT GAAGGACTGG 
GATGGCTGGG AGCCCCTGCA CGCCGCTGCC TTCTGGGGAC AGATGCAGAT GGCAGAGCTC 
TTGGTGTCCC ATGGGGCCAG TCTCAGTGCT AGGACGTCCA TGGACGAGAT GCCAATTGAC 
CTGTGTGAAG AGGAGGAGTT CAAAGTCCTG CTGTTGGAGC TGAAACACAA GCACGACGTG 
ATCATGAAGT CGCAGCTGAG GCATAAGTCA TCTTTGAGCA GGAGGACGTC CAGCGCGGGC 
AGCCGAGGGA AGGTGGTACG TCGAGCCAGC CTGTCCGACA GGACCAACCT GTACAGGAAG 
GAGTATGAGG GAGAGGCTAT ACTCTGGCAG CAGCGGAGTG CATCCGAGGA TCAGCGGAAC 
TCCACCTACA ACGGGGACAT CAGGGAGACC AGGACAGACC AAGAGAATAA GGACCCGAAC 
CCCAGGCTGG AGAAGCCCGT GCTGCTCTCC GAGTTTCCCA CCAAGATCCC ACACAGCGAC 
ATGGACATGC CTGTTGAGAA CGGCCTCCGG GCTCCAGTCA GCACCTACCA GTATGCGCTG 
TGTAACGGGG ACGTCTGGAA AGTGCACGAG GTGCCCGACT ACAGCATGGC CTATGGCAAC 
CCTGGCGTGG CCGATGCCAC CCCATCCTGG AGTGGCTACA AGGAACAGAG CCCCCAGACG 
CTTCTGGAGC TGAAGAGGCA GCGGGCTGCG GCCAAGCTGC TCAGCCACCC CTTCCTGAGC 
ACCCACCTGG GCAGTGGCGT GTCCAGGACA GGCGAGGGCA GCAGCGAAGG CAAGGCCCCC 
TTGATCGGAG GCAGAACTTC TCCGTACAGT AGCAACGGGA CCTCGGTGTA TTACACGGTC 
ACCAGTGGAG ACCCCCCACT CCTGAAGTTC AAGGCCCCCA TAGAGGAGAT GGAGGAGAAG 
GTCCATGGCT GCTGCCGCAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214553.1
CDS	229..1935
Translation	MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDLQHRKRKHERKRSTGGRRKKVSFEASVALLEASLRNDAEEVRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRAAPEQQMISDIHCMIAAGQDLDWVDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQQRSASEDQRNSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPHSDMDMPVENGLRAPVSTYQYALCNGDVWKVHEVPDYSMAYGNPGVADATPSWSGYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSGVSRTGEGSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	XM_005214496.3
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214496.3

ORF Insert Sequence:

ATGGCCAGCC ACGTGGACCT GCTGACCGAA CTGCAGCTGC TGGAGAAGGT GCCCACGTTG 
GAGCGGCTGC GCGCAGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAGTG GGCGCAGTAT 
GAGCAGGACC TGCAGCATCG CAAGCGCAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGG 
CGAAAGAAGG TGTCCTTCGA GGCCAGTGTG GCCCTCCTGG AGGCCTCGCT TAGGAACGAC 
GCTGAAGAAG TGCGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAACGAAGAT 
GGACTCACCG CCCTGCACCA GTGCTGCATC GACAACTTTG AGGAAATTGT CAAGCTGCTC 
CTTTCGCACG GTGCCAACGT GAACGCCAAG GACAACGAGC TGTGGACGCC CCTGCACGCC 
GCGGCCACCT GCGGCCACAT TAACCTGGTG AAAATCCTCG TTCAGTATGG AGCCGACTTG 
CTCGCTGTCA ACTCCGATGG GAACATGCCC TATGACCTCT GTGAGGATGA GCCCACCCTG 
GATGTTATCG AGACGTGCAT GGCGTACCAG GGCATCACCC AGGAGAAAAT CAACGAGATG 
CGGGCAGCTC CCGAGCAGCA GATGATTTCA GACATTCACT GTATGATCGC AGCTGGCCAG 
GACCTTGATT GGGTGGATGC CCAGGGGGCC ACACTGCTGC ACATAGCTGG AGCCAACGGA 
TACCTGCGGG CAGCCGAGCT CCTCCTGGAT CATGGTGTAC GCGTGGATGT GAAGGACTGG 
GATGGCTGGG AGCCCCTGCA CGCCGCTGCC TTCTGGGGAC AGATGCAGAT GGCAGAGCTC 
TTGGTGTCCC ATGGGGCCAG TCTCAGTGCT AGGACGTCCA TGGACGAGAT GCCAATTGAC 
CTGTGTGAAG AGGAGGAGTT CAAAGTCCTG CTGTTGGAGC TGAAACACAA GCACGACGTG 
ATCATGAAGT CGCAGCTGAG GCATAAGTCA TCTTTGAGCA GGAGGACGTC CAGCGCGGGC 
AGCCGAGGGA AGGTGGTACG TCGAGCCAGC CTGTCCGACA GGACCAACCT GTACAGGAAG 
GAGTATGAGG GAGAGGCTAT ACTCTGGCAG CAGCGGAGTG CATCCGAGGA TCAGCGGAAC 
TCCACCTACA ACGGGGACAT CAGGGAGACC AGGACAGACC AAGAGAATAA GGACCCGAAC 
CCCAGGCTGG AGAAGCCCGT GCTGCTCTCC GAGTTTCCCA CCAAGATCCC ACACAGCGAC 
ATGGACATGC CTGTTGAGAA CGGCCTCCGG GCTCCAGTCA GCACCTACCA GTATGCGCTG 
TGTAACGGGG ACGTCTGGAA AGTGCACGAG GTGCCCGACT ACAGCATGGC CTATGGCAAC 
CCTGGCGTGG CCGATGCCAC CCCATCCTGG AGTGGCTACA AGGAACAGAG CCCCCAGACG 
CTTCTGGAGC TGAAGAGGCA GCGGGCTGCG GCCAAGCTGC TCAGCCACCC CTTCCTGAGC 
ACCCACCTGG GCAGTGGCGT GTCCAGGACA GGCGAGGGCA GCAGCGAAGG CAAGGCCCCC 
TTGATCGGAG GCAGAACTTC TCCGTACAGT AGCAACGGGA CCTCGGTGTA TTACACGGTC 
ACCAGTGGAG ACCCCCCACT CCTGAAGTTC AAGGCCCCCA TAGAGGAGAT GGAGGAGAAG 
GTCCATGGCT GCTGCCGCAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R16B ( NM_174824.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777249.1 Bos taurus protein phosphatase 1 regulatory subunit 16B (PPP1R16B), mRNA.

Price & Availability↑

CloneID	OBa43944
Clone ID Related Accession (Same CDS sequence)	NM_174824.2
Accession Version	NM_174824.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Bos taurus(cattle)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF362909.1. On Jun 3, 2003 this sequence version replaced NM_174824.1. ##Evidence-Data-START## Transcript exon combination :: AF362909.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAGCC ACGTGGACCT GCTGACCGAA CTGCAGCTGC TGGAGAAGGT GCCCACGTTG 
GAGCGGCTGC GCGCAGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAGTG GGCGCAGTAT 
GAGCAGGACC TGCAGCATCG CAAGCGCAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGG 
CGAAAGAAGG TGTCCTTCGA GGCCAGTGTG GCCCTCCTGG AGGCCTCGCT TAGGAACGAC 
GCTGAAGAAG TGCGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAACGAAGAT 
GGACTCACCG CCCTGCACCA GTGCTGCATC GACAACTTTG AGGAAATTGT CAAGCTGCTC 
CTTTCGCACG GTGCCAACGT GAACGCCAAG GACAACGAGC TGTGGACGCC CCTGCACGCC 
GCGGCCACCT GCGGCCACAT TAACCTGGTG AAAATCCTCG TTCAGTATGG AGCCGACTTG 
CTCGCTGTCA ACTCCGATGG GAACATGCCC TATGACCTCT GTGAGGATGA GCCCACCCTG 
GATGTTATCG AGACGTGCAT GGCGTACCAG GGCATCACCC AGGAGAAAAT CAACGAGATG 
CGGGCAGCTC CCGAGCAGCA GATGATTTCA GACATTCATT GTATGATCGC AGCTGGCCAG 
GACCTTGACT GGGTGGATGC CCAGGGGGCC ACACTGCTGC ACATAGCTGG AGCCAACGGA 
TACCTGCGGG CAGCCGAGCT CCTCCTGGAT CATGGTGTAC GCGTGGATGT GAAGGACTGG 
GATGGCTGGG AGCCCCTGCA CGCCGCTGCC TTCTGGGGAC AGATGCAGAT GGCAGAGCTG 
TTGGTGTCCC ATGGGGCCAG TCTCAGTGCT AGGACGTCCA TGGACGAGAT GCCAATTGAC 
CTGTGTGAAG AGGAGGAGTT CAAAGTCCTG CTGTTGGAGC TGAAACACAA GCACGACGTG 
ATCATGAAGT CGCAGCTGAG GCATAAGTCA TCTTTGAGCA GGAGGACGTC CAGCGCGGGC 
AGCCGAGGGA AGGTGGTACG TCGAGCCAGC CTGTCCGACA GGACCAACCT GTACAGGAAG 
GAGTATGAGG GAGAGGCTAT ACTCTGGCAG CAGCGGAGTG CATCCGAGGA TCAGCGGAAC 
TCCACCTACA ACGGGGACAT CAGGGAGACC AGGACAGACC AAGAGAATAA GGACCCGAAC 
CCCAGGCTGG AGAAGCCCGT GCTGCTCTCC GAGTTTCCCA CCAAGATCCC ACACAGCGAC 
ATGGACATGC CTGTTGAGAA CGGCCTCCGG GCTCCAGTCA GCACCTACCA GTATGCGCTG 
TGTAACGGGG ACGTCTGGAA AGTGCACGAG GTGCCCGACT ACAGCATGGC CTATGGCAAC 
CCTGGCGTGG CCGATGCCAC CCCATCCTGG AGTGGCTACA AGGAACAGAG CCCCCAGACG 
CTTCTGGAGC TGAAGAGGCA GCGGGCTGCG GCCAAGCTGC TCAGCCACCC CTTCCTGAGC 
ACCCACCTGG GCAGTGGCGT GTCCAGGACG GGCGAGGGCA GCAGCGAAGG CAAGGCCCCC 
TTGATCGGAG GCAGAACTTC TCCGTACAGT AGCAATGGGA CCTCGGTGTA TTACACGGTC 
ACCAGTGGAG ACCCCCCACT CCTGAAGTTC AAGGCCCCCA TAGAGGAGAT GGAGGAGAAG 
GTCCATGGCT GCTGCCGCAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777249.1
CDS	186..1892
Translation	MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDLQHRKRKHERKRSTGGRRKKVSFEASVALLEASLRNDAEEVRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRAAPEQQMISDIHCMIAAGQDLDWVDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQQRSASEDQRNSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPHSDMDMPVENGLRAPVSTYQYALCNGDVWKVHEVPDYSMAYGNPGVADATPSWSGYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSGVSRTGEGSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	NM_174824.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase 1 regulatory subunit 16B (PPP1R16B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174824.2

ORF Insert Sequence:

ATGGCCAGCC ACGTGGACCT GCTGACCGAA CTGCAGCTGC TGGAGAAGGT GCCCACGTTG 
GAGCGGCTGC GCGCAGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAGTG GGCGCAGTAT 
GAGCAGGACC TGCAGCATCG CAAGCGCAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGG 
CGAAAGAAGG TGTCCTTCGA GGCCAGTGTG GCCCTCCTGG AGGCCTCGCT TAGGAACGAC 
GCTGAAGAAG TGCGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAACGAAGAT 
GGACTCACCG CCCTGCACCA GTGCTGCATC GACAACTTTG AGGAAATTGT CAAGCTGCTC 
CTTTCGCACG GTGCCAACGT GAACGCCAAG GACAACGAGC TGTGGACGCC CCTGCACGCC 
GCGGCCACCT GCGGCCACAT TAACCTGGTG AAAATCCTCG TTCAGTATGG AGCCGACTTG 
CTCGCTGTCA ACTCCGATGG GAACATGCCC TATGACCTCT GTGAGGATGA GCCCACCCTG 
GATGTTATCG AGACGTGCAT GGCGTACCAG GGCATCACCC AGGAGAAAAT CAACGAGATG 
CGGGCAGCTC CCGAGCAGCA GATGATTTCA GACATTCATT GTATGATCGC AGCTGGCCAG 
GACCTTGACT GGGTGGATGC CCAGGGGGCC ACACTGCTGC ACATAGCTGG AGCCAACGGA 
TACCTGCGGG CAGCCGAGCT CCTCCTGGAT CATGGTGTAC GCGTGGATGT GAAGGACTGG 
GATGGCTGGG AGCCCCTGCA CGCCGCTGCC TTCTGGGGAC AGATGCAGAT GGCAGAGCTG 
TTGGTGTCCC ATGGGGCCAG TCTCAGTGCT AGGACGTCCA TGGACGAGAT GCCAATTGAC 
CTGTGTGAAG AGGAGGAGTT CAAAGTCCTG CTGTTGGAGC TGAAACACAA GCACGACGTG 
ATCATGAAGT CGCAGCTGAG GCATAAGTCA TCTTTGAGCA GGAGGACGTC CAGCGCGGGC 
AGCCGAGGGA AGGTGGTACG TCGAGCCAGC CTGTCCGACA GGACCAACCT GTACAGGAAG 
GAGTATGAGG GAGAGGCTAT ACTCTGGCAG CAGCGGAGTG CATCCGAGGA TCAGCGGAAC 
TCCACCTACA ACGGGGACAT CAGGGAGACC AGGACAGACC AAGAGAATAA GGACCCGAAC 
CCCAGGCTGG AGAAGCCCGT GCTGCTCTCC GAGTTTCCCA CCAAGATCCC ACACAGCGAC 
ATGGACATGC CTGTTGAGAA CGGCCTCCGG GCTCCAGTCA GCACCTACCA GTATGCGCTG 
TGTAACGGGG ACGTCTGGAA AGTGCACGAG GTGCCCGACT ACAGCATGGC CTATGGCAAC 
CCTGGCGTGG CCGATGCCAC CCCATCCTGG AGTGGCTACA AGGAACAGAG CCCCCAGACG 
CTTCTGGAGC TGAAGAGGCA GCGGGCTGCG GCCAAGCTGC TCAGCCACCC CTTCCTGAGC 
ACCCACCTGG GCAGTGGCGT GTCCAGGACG GGCGAGGGCA GCAGCGAAGG CAAGGCCCCC 
TTGATCGGAG GCAGAACTTC TCCGTACAGT AGCAATGGGA CCTCGGTGTA TTACACGGTC 
ACCAGTGGAG ACCCCCCACT CCTGAAGTTC AAGGCCCCCA TAGAGGAGAT GGAGGAGAAG 
GTCCATGGCT GCTGCCGCAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TIMAP-protein phosphatase 1-complex controls endothelin-1 production via ECE-1 dephosphorylation.
The international journal of biochemistry & cell biology7311-18(2016 Apr)
Boratk? A,Ver?b Z,Petrovski G,Csortos C

Multi-directional function of the protein phosphatase 1 regulatory subunit TIMAP.
Biochemical and biophysical research communications435(4)567-73(2013 Jun)
Shopik MJ,Li L,Luu HA,Obeidat M,Holmes CF,Ballermann BJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Phosphorylation of TIMAP by glycogen synthase kinase-3beta activates its associated protein phosphatase 1.
The Journal of biological chemistry282(35)25960-9(2007 Aug)
Li L,Kozlowski K,Wegner B,Rashid T,Yeung T,Holmes C,Ballermann BJ

TIMAP, a novel CAAX box protein regulated by TGF-beta1 and expressed in endothelial cells.
American journal of physiology. Cell physiology283(1)C327-37(2002 Jul)
Cao W,Mattagajasingh SN,Xu H,Kim K,Fierlbeck W,Deng J,Lowenstein CJ,Ballermann BJ

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PPP1R16B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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