PPP1R16B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP1R16B
Entrez Gene ID	26051
Full Name	protein phosphatase 1 regulatory subunit 16B
Synonyms	ANKRD4,TIMAP
General protein information	Preferred Names protein phosphatase 1 regulatory subunit 16B Names protein phosphatase 1 regulatory inhibitor subunit 16B CAAX box protein TIMAP TGF-beta-inhibited membrane-associated protein ankyrin repeat domain protein 4 ankyrin repeat domain-containing protein 4 hTIMAP protein phosphatase 1, regulatory (inhibitor) subunit 16B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q11.23
Summary	The protein encoded by this gene is membrane-associated and contains five ankyrin repeats, a protein phosphatase-1-interacting domain, and a carboxy-terminal CAAX box domain. Synthesis of the encoded protein is inhibited by transforming growth factor beta-1. The protein may bind to the membrane through its CAAX box domain and may act as a signaling molecule through interaction with protein phosphatase-1. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015].
Disorder MIM:	613275

mRNA and Protein(s)

mRNA	Protein	Name
XM_011528768.3	XP_011527070.1	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X1
XM_011528769.2	XP_011527071.1	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X2
XM_011528768.2	XP_011527070.1	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X1
NM_001172735.3	NP_001166206.1	protein phosphatase 1 regulatory inhibitor subunit 16B isoform 2
NM_015568.4	NP_056383.1	protein phosphatase 1 regulatory inhibitor subunit 16B isoform 1 precursor
XM_017027785.1	XP_016883274.1	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X2
NM_001172735.2	NP_001166206.1	protein phosphatase 1 regulatory inhibitor subunit 16B isoform 2
NM_015568.3	NP_056383.1	protein phosphatase 1 regulatory inhibitor subunit 16B isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPP1R16B	NP_056383.1
Gallus gallus (chicken)	PPP1R16B	NP_001026022.1
Xenopus tropicalis (tropical clawed frog)	ppp1r16b	NP_001123752.1
Mus musculus (house mouse)	Ppp1r16b	NP_694729.1
Pan troglodytes (chimpanzee)	PPP1R16B	XP_003317035.1
Rattus norvegicus (Norway rat)	Ppp1r16b	NP_001178001.1
Danio rerio (zebrafish)	LOC799771	XP_001340092.5
Canis lupus familiaris (dog)	PPP1R16B	XP_003433312.1
Bos taurus (cattle)	PPP1R16B	NP_777249.1

Gene Ontology
Bibliography

Related articles in PubMed

TIMAP, the versatile protein phosphatase 1 regulator in endothelial cells.
Boratk? A, Csortos C
IUBMB life69(12)918-928(2017 Dec)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Yadav L, Tamene F, G??s H, van Drogen A, Katainen R, Aebersold R, Gstaiger M, Varjosalo M
Cell systems4(4)430-444.e5(2017 Apr)

PKC mediated phosphorylation of TIMAP regulates PP1c activity and endothelial barrier function.
Boratk? A, Csortos C
Biochimica et biophysica acta1864(2)431-439(2017 02)

Regulation of merlin by protein phosphatase 1-TIMAP and EBP50 in endothelial cells.
Boratk? A, P?ter M, Csortos C
The international journal of biochemistry & cell biology8210-17(2017 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

phosphorylation of TIMAP on Ser331 by PKC represents a new mechanism of endothelial barrier regulation, through the inhibition of phospho-ERM dephosphorylation
Title: PKC mediated phosphorylation of TIMAP regulates PP1c activity and endothelial barrier function.

NF2 localizes in nucleus when Ser518 is not phosphorylated, while phosphorylated form is present in cytoplasm and plasma membrane. Data suggest that binding of NF2 to TIMAP and EBP50 is critical in nuclear localization of NF2. (NF2 = neurofibromin 2; TIMAP = TGF-beta-inhibited membrane-associated protein; EBP50 = Ezrin-Radixin-Mosein binding phosphoprotein 50)
Title: Regulation of merlin by protein phosphatase 1-TIMAP and EBP50 in endothelial cells.

Results indicate that PKC-phosphorylated ECE-1 is a TIMAP-PP1c substrate and this phosphatase complex has an important role in endothelin-1 production of EC through the regulation of ECE-1 activity.
Title: TIMAP-protein phosphatase 1-complex controls endothelin-1 production via ECE-1 dephosphorylation.

ROCK phosphorylated eEF1A1 is a novel substrate for TIMAP-PP1 underlining the complex regulatory role of TIMAP in the endothelium.
Title: Elongation factor-1A1 is a novel substrate of the protein phosphatase 1-TIMAP complex.

TIMAP promotes angiogenesis by suppressing PTEN-mediated Akt inhibition in human glomerular endothelial cells.
Title: TIMAP promotes angiogenesis by suppressing PTEN-mediated Akt inhibition in human glomerular endothelial cells.

The following PPP1R16B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R16B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31169	NM_015568.4 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu66754	XM_011528769.2 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu66754	XM_017027785.1 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu32242	NM_001172735.3 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu66753	XM_011528768.3 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu66753	XM_011528768.2	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu32242	NM_001172735.2	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu31169	NM_015568.3	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP1R16B ( NM_015568.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056383.1 Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31169
Clone ID Related Accession (Same CDS sequence)	NM_015568.4 , NM_015568.3
Accession Version	NM_015568.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY125679.1, AB020630.2, AB177855.1 and BC152467.1. On Nov 22, 2018 this sequence version replaced NM_015568.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152467.1, AB020630.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000299824.6/ ENSP00000299824.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAGTC ACGTGGACCT GCTGACGGAG CTGCAGCTGC TGGAGAAGGT GCCCACGCTG 
GAGCGGCTGC GGGCTGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAATG GGCACAGTAC 
GAGCAGGACT TGCAGCACCG CAAGCGAAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGC 
CGCAAGAAAG TGTCCTTCGA GGCCAGCGTG GCCCTGCTGG AGGCCTCGCT GAGGAACGAC 
GCCGAGGAAG TACGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAATGAGGAC 
GGACTCACAG CCCTACACCA GTGCTGCATC GACAACTTTG AGGAAATTGT GAAGCTGCTC 
CTCTCCCATG GTGCCAATGT GAACGCCAAG GACAACGAGC TGTGGACACC TCTCCATGCT 
GCAGCCACCT GCGGCCACAT CAACCTGGTG AAGATCCTCG TTCAGTATGG GGCCGACTTG 
CTTGCTGTCA ACTCGGATGG GAACATGCCA TATGACCTCT GCGAGGATGA ACCCACCCTG 
GATGTCATCG AGACCTGCAT GGCATACCAG GGCATCACCC AAGAGAAAAT CAACGAGATG 
CGGGTGGCTC CTGAGCAGCA GATGATTGCG GACATCCACT GCATGATCGC AGCGGGCCAG 
GACCTGGACT GGATAGATGC CCAGGGTGCC ACACTGCTGC ACATAGCTGG AGCCAATGGA 
TACCTGCGGG CAGCTGAGCT CCTCCTGGAC CATGGAGTGC GTGTGGATGT GAAGGACTGG 
GATGGCTGGG AGCCCCTGCA TGCAGCTGCC TTCTGGGGAC AGATGCAGAT GGCAGAGCTA 
TTGGTGTCCC ATGGAGCTAG TCTCAGTGCA AGGACATCCA TGGATGAGAT GCCAATAGAC 
CTGTGCGAGG AGGAAGAGTT CAAGGTCCTG CTGCTGGAGC TAAAACACAA GCATGATGTG 
ATCATGAAGT CACAGCTGAG GCACAAGTCA TCCTTGAGCC GGAGGACCTC CAGCGCAGGC 
AGCCGTGGGA AGGTGGTGCG GCGAGCCAGC CTGTCGGACA GGACCAACCT GTATAGGAAG 
GAGTATGAGG GAGAGGCCAT CCTGTGGCAG CGGAGTGCAG CTGAGGATCA GCGGACCTCC 
ACCTACAACG GGGACATCAG GGAGACCAGG ACAGACCAAG AGAATAAGGA CCCTAACCCC 
AGGCTGGAGA AGCCCGTGCT ACTCTCCGAA TTTCCTACCA AGATCCCACG AGGTGAACTG 
GACATGCCTG TTGAGAATGG CCTCCGGGCT CCGGTCAGTG CCTACCAGTA TGCGCTGGCC 
AACGGGGATG TCTGGAAGGT GCATGAGGTG CCTGACTACA GCATGGCCTA TGGCAACCCT 
GGCGTGGCCG ACGCCACCCC GCCCTGGAGC AGCTACAAGG AACAGAGCCC TCAGACGCTT 
CTGGAGCTGA AGCGGCAGCG GGCTGCAGCC AAGCTGCTCA GCCACCCCTT CCTTAGCACA 
CACCTGGGCA GCAGCATGGC CAGGACGGGC GAGAGTAGCA GTGAAGGCAA GGCCCCCTTG 
ATCGGAGGCA GAACTTCACC GTACAGCAGC AATGGGACCT CGGTATATTA CACGGTCACC 
AGCGGAGATC CCCCACTCTT AAAGTTCAAG GCCCCCATAG AGGAGATGGA GGAGAAGGTG 
CATGGCTGTT GCCGTATCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056383.1
CDS	198..1901
Translation	MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDLQHRKRKHERKRSTGGRRKKVSFEASVALLEASLRNDAEEVRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRVAPEQQMIADIHCMIAAGQDLDWIDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEVPDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMARTGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	NM_015568.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015568.4

ORF Insert Sequence:

ATGGCCAGTC ACGTGGACCT GCTGACGGAG CTGCAGCTGC TGGAGAAGGT GCCCACGCTG 
GAGCGGCTGC GGGCTGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAATG GGCACAGTAC 
GAGCAGGACT TGCAGCACCG CAAGCGAAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGC 
CGCAAGAAAG TGTCCTTCGA GGCCAGCGTG GCCCTGCTGG AGGCCTCGCT GAGGAACGAC 
GCCGAGGAAG TACGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAATGAGGAC 
GGACTCACAG CCCTACACCA GTGCTGCATC GACAACTTTG AGGAAATTGT GAAGCTGCTC 
CTCTCCCATG GTGCCAATGT GAACGCCAAG GACAACGAGC TGTGGACACC TCTCCATGCT 
GCAGCCACCT GCGGCCACAT CAACCTGGTG AAGATCCTCG TTCAGTATGG GGCCGACTTG 
CTTGCTGTCA ACTCGGATGG GAACATGCCA TATGACCTCT GCGAGGATGA ACCCACCCTG 
GATGTCATCG AGACCTGCAT GGCATACCAG GGCATCACCC AAGAGAAAAT CAACGAGATG 
CGGGTGGCTC CTGAGCAGCA GATGATTGCG GACATCCACT GCATGATCGC AGCGGGCCAG 
GACCTGGACT GGATAGATGC CCAGGGTGCC ACACTGCTGC ACATAGCTGG AGCCAATGGA 
TACCTGCGGG CAGCTGAGCT CCTCCTGGAC CATGGAGTGC GTGTGGATGT GAAGGACTGG 
GATGGCTGGG AGCCCCTGCA TGCAGCTGCC TTCTGGGGAC AGATGCAGAT GGCAGAGCTA 
TTGGTGTCCC ATGGAGCTAG TCTCAGTGCA AGGACATCCA TGGATGAGAT GCCAATAGAC 
CTGTGCGAGG AGGAAGAGTT CAAGGTCCTG CTGCTGGAGC TAAAACACAA GCATGATGTG 
ATCATGAAGT CACAGCTGAG GCACAAGTCA TCCTTGAGCC GGAGGACCTC CAGCGCAGGC 
AGCCGTGGGA AGGTGGTGCG GCGAGCCAGC CTGTCGGACA GGACCAACCT GTATAGGAAG 
GAGTATGAGG GAGAGGCCAT CCTGTGGCAG CGGAGTGCAG CTGAGGATCA GCGGACCTCC 
ACCTACAACG GGGACATCAG GGAGACCAGG ACAGACCAAG AGAATAAGGA CCCTAACCCC 
AGGCTGGAGA AGCCCGTGCT ACTCTCCGAA TTTCCTACCA AGATCCCACG AGGTGAACTG 
GACATGCCTG TTGAGAATGG CCTCCGGGCT CCGGTCAGTG CCTACCAGTA TGCGCTGGCC 
AACGGGGATG TCTGGAAGGT GCATGAGGTG CCTGACTACA GCATGGCCTA TGGCAACCCT 
GGCGTGGCCG ACGCCACCCC GCCCTGGAGC AGCTACAAGG AACAGAGCCC TCAGACGCTT 
CTGGAGCTGA AGCGGCAGCG GGCTGCAGCC AAGCTGCTCA GCCACCCCTT CCTTAGCACA 
CACCTGGGCA GCAGCATGGC CAGGACGGGC GAGAGTAGCA GTGAAGGCAA GGCCCCCTTG 
ATCGGAGGCA GAACTTCACC GTACAGCAGC AATGGGACCT CGGTATATTA CACGGTCACC 
AGCGGAGATC CCCCACTCTT AAAGTTCAAG GCCCCCATAG AGGAGATGGA GGAGAAGGTG 
CATGGCTGTT GCCGTATCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R16B ( NM_015568.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056383.1 Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31169
Clone ID Related Accession (Same CDS sequence)	NM_015568.4 , NM_015568.3
Accession Version	NM_015568.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY125679.1, AB020630.2, AB177855.1 and BC152467.1. On Sep 29, 2015 this sequence version replaced NM_015568.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152467.1, AB020630.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGTC ACGTGGACCT GCTGACGGAG CTGCAGCTGC TGGAGAAGGT GCCCACGCTG 
GAGCGGCTGC GGGCTGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAATG GGCACAGTAC 
GAGCAGGACT TGCAGCACCG CAAGCGAAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGC 
CGCAAGAAAG TGTCCTTCGA GGCCAGCGTG GCCCTGCTGG AGGCCTCGCT GAGGAACGAC 
GCCGAGGAAG TACGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAATGAGGAC 
GGACTCACAG CCCTACACCA GTGCTGCATC GACAACTTTG AGGAAATTGT GAAGCTGCTC 
CTCTCCCATG GTGCCAATGT GAACGCCAAG GACAACGAGC TGTGGACACC TCTCCATGCT 
GCAGCCACCT GCGGCCACAT CAACCTGGTG AAGATCCTCG TTCAGTATGG GGCCGACTTG 
CTTGCTGTCA ACTCGGATGG GAACATGCCA TATGACCTCT GCGAGGATGA ACCCACCCTG 
GATGTCATCG AGACCTGCAT GGCATACCAG GGCATCACCC AAGAGAAAAT CAACGAGATG 
CGGGTGGCTC CTGAGCAGCA GATGATTGCG GACATCCACT GCATGATCGC AGCGGGCCAG 
GACCTGGACT GGATAGATGC CCAGGGTGCC ACACTGCTGC ACATAGCTGG AGCCAATGGA 
TACCTGCGGG CAGCTGAGCT CCTCCTGGAC CATGGAGTGC GTGTGGATGT GAAGGACTGG 
GATGGCTGGG AGCCCCTGCA TGCAGCTGCC TTCTGGGGAC AGATGCAGAT GGCAGAGCTA 
TTGGTGTCCC ATGGAGCTAG TCTCAGTGCA AGGACATCCA TGGATGAGAT GCCAATAGAC 
CTGTGCGAGG AGGAAGAGTT CAAGGTCCTG CTGCTGGAGC TAAAACACAA GCATGATGTG 
ATCATGAAGT CACAGCTGAG GCACAAGTCA TCCTTGAGCC GGAGGACCTC CAGCGCAGGC 
AGCCGTGGGA AGGTGGTGCG GCGAGCCAGC CTGTCGGACA GGACCAACCT GTATAGGAAG 
GAGTATGAGG GAGAGGCCAT CCTGTGGCAG CGGAGTGCAG CTGAGGATCA GCGGACCTCC 
ACCTACAACG GGGACATCAG GGAGACCAGG ACAGACCAAG AGAATAAGGA CCCTAACCCC 
AGGCTGGAGA AGCCCGTGCT ACTCTCCGAA TTTCCTACCA AGATCCCACG AGGTGAACTG 
GACATGCCTG TTGAGAATGG CCTCCGGGCT CCGGTCAGTG CCTACCAGTA TGCGCTGGCC 
AACGGGGATG TCTGGAAGGT GCATGAGGTG CCTGACTACA GCATGGCCTA TGGCAACCCT 
GGCGTGGCCG ACGCCACCCC GCCCTGGAGC AGCTACAAGG AACAGAGCCC TCAGACGCTT 
CTGGAGCTGA AGCGGCAGCG GGCTGCAGCC AAGCTGCTCA GCCACCCCTT CCTTAGCACA 
CACCTGGGCA GCAGCATGGC CAGGACGGGC GAGAGTAGCA GTGAAGGCAA GGCCCCCTTG 
ATCGGAGGCA GAACTTCACC GTACAGCAGC AATGGGACCT CGGTATATTA CACGGTCACC 
AGCGGAGATC CCCCACTCTT AAAGTTCAAG GCCCCCATAG AGGAGATGGA GGAGAAGGTG 
CATGGCTGTT GCCGTATCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056383.1
CDS	202..1905
Translation	MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDLQHRKRKHERKRSTGGRRKKVSFEASVALLEASLRNDAEEVRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRVAPEQQMIADIHCMIAAGQDLDWIDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEVPDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMARTGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	NM_015568.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015568.3

ORF Insert Sequence:

ATGGCCAGTC ACGTGGACCT GCTGACGGAG CTGCAGCTGC TGGAGAAGGT GCCCACGCTG 
GAGCGGCTGC GGGCTGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAATG GGCACAGTAC 
GAGCAGGACT TGCAGCACCG CAAGCGAAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGC 
CGCAAGAAAG TGTCCTTCGA GGCCAGCGTG GCCCTGCTGG AGGCCTCGCT GAGGAACGAC 
GCCGAGGAAG TACGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAATGAGGAC 
GGACTCACAG CCCTACACCA GTGCTGCATC GACAACTTTG AGGAAATTGT GAAGCTGCTC 
CTCTCCCATG GTGCCAATGT GAACGCCAAG GACAACGAGC TGTGGACACC TCTCCATGCT 
GCAGCCACCT GCGGCCACAT CAACCTGGTG AAGATCCTCG TTCAGTATGG GGCCGACTTG 
CTTGCTGTCA ACTCGGATGG GAACATGCCA TATGACCTCT GCGAGGATGA ACCCACCCTG 
GATGTCATCG AGACCTGCAT GGCATACCAG GGCATCACCC AAGAGAAAAT CAACGAGATG 
CGGGTGGCTC CTGAGCAGCA GATGATTGCG GACATCCACT GCATGATCGC AGCGGGCCAG 
GACCTGGACT GGATAGATGC CCAGGGTGCC ACACTGCTGC ACATAGCTGG AGCCAATGGA 
TACCTGCGGG CAGCTGAGCT CCTCCTGGAC CATGGAGTGC GTGTGGATGT GAAGGACTGG 
GATGGCTGGG AGCCCCTGCA TGCAGCTGCC TTCTGGGGAC AGATGCAGAT GGCAGAGCTA 
TTGGTGTCCC ATGGAGCTAG TCTCAGTGCA AGGACATCCA TGGATGAGAT GCCAATAGAC 
CTGTGCGAGG AGGAAGAGTT CAAGGTCCTG CTGCTGGAGC TAAAACACAA GCATGATGTG 
ATCATGAAGT CACAGCTGAG GCACAAGTCA TCCTTGAGCC GGAGGACCTC CAGCGCAGGC 
AGCCGTGGGA AGGTGGTGCG GCGAGCCAGC CTGTCGGACA GGACCAACCT GTATAGGAAG 
GAGTATGAGG GAGAGGCCAT CCTGTGGCAG CGGAGTGCAG CTGAGGATCA GCGGACCTCC 
ACCTACAACG GGGACATCAG GGAGACCAGG ACAGACCAAG AGAATAAGGA CCCTAACCCC 
AGGCTGGAGA AGCCCGTGCT ACTCTCCGAA TTTCCTACCA AGATCCCACG AGGTGAACTG 
GACATGCCTG TTGAGAATGG CCTCCGGGCT CCGGTCAGTG CCTACCAGTA TGCGCTGGCC 
AACGGGGATG TCTGGAAGGT GCATGAGGTG CCTGACTACA GCATGGCCTA TGGCAACCCT 
GGCGTGGCCG ACGCCACCCC GCCCTGGAGC AGCTACAAGG AACAGAGCCC TCAGACGCTT 
CTGGAGCTGA AGCGGCAGCG GGCTGCAGCC AAGCTGCTCA GCCACCCCTT CCTTAGCACA 
CACCTGGGCA GCAGCATGGC CAGGACGGGC GAGAGTAGCA GTGAAGGCAA GGCCCCCTTG 
ATCGGAGGCA GAACTTCACC GTACAGCAGC AATGGGACCT CGGTATATTA CACGGTCACC 
AGCGGAGATC CCCCACTCTT AAAGTTCAAG GCCCCCATAG AGGAGATGGA GGAGAAGGTG 
CATGGCTGTT GCCGTATCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R16B ( XM_011528769.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527071.1 Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X3, mRNA.

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CloneID	OHu66754
Clone ID Related Accession (Same CDS sequence)	XM_011528769.2 , XM_017027785.1
Accession Version	XM_011528769.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTGG CTCCTGAGCA GCAGATGATT GCGGACATCC ACTGCATGAT CGCAGCGGGC 
CAGGACCTGG ACTGGATAGA TGCCCAGGGT GCCACACTGC TGCACATAGC TGGAGCCAAT 
GGATACCTGC GGGCAGCTGA GCTCCTCCTG GACCATGGAG TGCGTGTGGA TGTGAAGGAC 
TGGGATGGCT GGGAGCCCCT GCATGCAGCT GCCTTCTGGG GACAGATGCA GATGGCAGAG 
CTATTGGTGT CCCATGGAGC TAGTCTCAGT GCAAGGACAT CCATGGATGA GATGCCAATA 
GACCTGTGCG AGGAGGAAGA GTTCAAGGTC CTGCTGCTGG AGCTAAAACA CAAGCATGAT 
GTGATCATGA AGTCACAGCT GAGGCACAAG TCATCCTTGA GCCGGAGGAC CTCCAGCGCA 
GGCAGCCGTG GGAAGGTGGT GCGGCGAGCC AGCCTGTCGG ACAGGACCAA CCTGTATAGG 
AAGGAGTATG AGGGAGAGGC CATCCTGTGG CAGCGGAGTG CAGCTGAGGA TCAGCGGACC 
TCCACCTACA ACGGGGACAT CAGGGAGACC AGGACAGACC AAGAGAATAA GGACCCTAAC 
CCCAGGCTGG AGAAGCCCGT GCTACTCTCC GAATTTCCTA CCAAGATCCC ACGAGGTGAA 
CTGGACATGC CTGTTGAGAA TGGCCTCCGG GCTCCGGTCA GTGCCTACCA GTATGCGCTG 
GCCAACGGGG ATGTCTGGAA GGTGCATGAG GTGCCTGACT ACAGCATGGC CTATGGCAAC 
CCTGGCGTGG CCGACGCCAC CCCGCCCTGG AGCAGCTACA AGGAACAGAG CCCTCAGACG 
CTTCTGGAGC TGAAGCGGCA GCGGGCTGCA GCCAAGCTGC TCAGCCACCC CTTCCTTAGC 
ACACACCTGG GCAGCAGCAT GGCCAGGACG GGCGAGAGTA GCAGTGAAGG CAAGGCCCCC 
TTGATCGGAG GCAGAACTTC ACCGTACAGC AGCAATGGGA CCTCGGTATA TTACACGGTC 
ACCAGCGGAG ATCCCCCACT CTTAAAGTTC AAGGCCCCCA TAGAGGAGAT GGAGGAGAAG 
GTGCATGGCT GTTGCCGTAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527071.1
CDS	380..1486
Translation	MRVAPEQQMIADIHCMIAAGQDLDWIDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEVPDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMARTGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	XM_011528769.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528769.2

ORF Insert Sequence:

ATGCGGGTGG CTCCTGAGCA GCAGATGATT GCGGACATCC ACTGCATGAT CGCAGCGGGC 
CAGGACCTGG ACTGGATAGA TGCCCAGGGT GCCACACTGC TGCACATAGC TGGAGCCAAT 
GGATACCTGC GGGCAGCTGA GCTCCTCCTG GACCATGGAG TGCGTGTGGA TGTGAAGGAC 
TGGGATGGCT GGGAGCCCCT GCATGCAGCT GCCTTCTGGG GACAGATGCA GATGGCAGAG 
CTATTGGTGT CCCATGGAGC TAGTCTCAGT GCAAGGACAT CCATGGATGA GATGCCAATA 
GACCTGTGCG AGGAGGAAGA GTTCAAGGTC CTGCTGCTGG AGCTAAAACA CAAGCATGAT 
GTGATCATGA AGTCACAGCT GAGGCACAAG TCATCCTTGA GCCGGAGGAC CTCCAGCGCA 
GGCAGCCGTG GGAAGGTGGT GCGGCGAGCC AGCCTGTCGG ACAGGACCAA CCTGTATAGG 
AAGGAGTATG AGGGAGAGGC CATCCTGTGG CAGCGGAGTG CAGCTGAGGA TCAGCGGACC 
TCCACCTACA ACGGGGACAT CAGGGAGACC AGGACAGACC AAGAGAATAA GGACCCTAAC 
CCCAGGCTGG AGAAGCCCGT GCTACTCTCC GAATTTCCTA CCAAGATCCC ACGAGGTGAA 
CTGGACATGC CTGTTGAGAA TGGCCTCCGG GCTCCGGTCA GTGCCTACCA GTATGCGCTG 
GCCAACGGGG ATGTCTGGAA GGTGCATGAG GTGCCTGACT ACAGCATGGC CTATGGCAAC 
CCTGGCGTGG CCGACGCCAC CCCGCCCTGG AGCAGCTACA AGGAACAGAG CCCTCAGACG 
CTTCTGGAGC TGAAGCGGCA GCGGGCTGCA GCCAAGCTGC TCAGCCACCC CTTCCTTAGC 
ACACACCTGG GCAGCAGCAT GGCCAGGACG GGCGAGAGTA GCAGTGAAGG CAAGGCCCCC 
TTGATCGGAG GCAGAACTTC ACCGTACAGC AGCAATGGGA CCTCGGTATA TTACACGGTC 
ACCAGCGGAG ATCCCCCACT CTTAAAGTTC AAGGCCCCCA TAGAGGAGAT GGAGGAGAAG 
GTGCATGGCT GTTGCCGTAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R16B ( XM_017027785.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883274.1 Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X2, mRNA.

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CloneID	OHu66754
Clone ID Related Accession (Same CDS sequence)	XM_011528769.2 , XM_017027785.1
Accession Version	XM_017027785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTGG CTCCTGAGCA GCAGATGATT GCGGACATCC ACTGCATGAT CGCAGCGGGC 
CAGGACCTGG ACTGGATAGA TGCCCAGGGT GCCACACTGC TGCACATAGC TGGAGCCAAT 
GGATACCTGC GGGCAGCTGA GCTCCTCCTG GACCATGGAG TGCGTGTGGA TGTGAAGGAC 
TGGGATGGCT GGGAGCCCCT GCATGCAGCT GCCTTCTGGG GACAGATGCA GATGGCAGAG 
CTATTGGTGT CCCATGGAGC TAGTCTCAGT GCAAGGACAT CCATGGATGA GATGCCAATA 
GACCTGTGCG AGGAGGAAGA GTTCAAGGTC CTGCTGCTGG AGCTAAAACA CAAGCATGAT 
GTGATCATGA AGTCACAGCT GAGGCACAAG TCATCCTTGA GCCGGAGGAC CTCCAGCGCA 
GGCAGCCGTG GGAAGGTGGT GCGGCGAGCC AGCCTGTCGG ACAGGACCAA CCTGTATAGG 
AAGGAGTATG AGGGAGAGGC CATCCTGTGG CAGCGGAGTG CAGCTGAGGA TCAGCGGACC 
TCCACCTACA ACGGGGACAT CAGGGAGACC AGGACAGACC AAGAGAATAA GGACCCTAAC 
CCCAGGCTGG AGAAGCCCGT GCTACTCTCC GAATTTCCTA CCAAGATCCC ACGAGGTGAA 
CTGGACATGC CTGTTGAGAA TGGCCTCCGG GCTCCGGTCA GTGCCTACCA GTATGCGCTG 
GCCAACGGGG ATGTCTGGAA GGTGCATGAG GTGCCTGACT ACAGCATGGC CTATGGCAAC 
CCTGGCGTGG CCGACGCCAC CCCGCCCTGG AGCAGCTACA AGGAACAGAG CCCTCAGACG 
CTTCTGGAGC TGAAGCGGCA GCGGGCTGCA GCCAAGCTGC TCAGCCACCC CTTCCTTAGC 
ACACACCTGG GCAGCAGCAT GGCCAGGACG GGCGAGAGTA GCAGTGAAGG CAAGGCCCCC 
TTGATCGGAG GCAGAACTTC ACCGTACAGC AGCAATGGGA CCTCGGTATA TTACACGGTC 
ACCAGCGGAG ATCCCCCACT CTTAAAGTTC AAGGCCCCCA TAGAGGAGAT GGAGGAGAAG 
GTGCATGGCT GTTGCCGTAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883274.1
CDS	694..1800
Translation	MRVAPEQQMIADIHCMIAAGQDLDWIDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEVPDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMARTGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	XM_017027785.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027785.1

ORF Insert Sequence:

ATGCGGGTGG CTCCTGAGCA GCAGATGATT GCGGACATCC ACTGCATGAT CGCAGCGGGC 
CAGGACCTGG ACTGGATAGA TGCCCAGGGT GCCACACTGC TGCACATAGC TGGAGCCAAT 
GGATACCTGC GGGCAGCTGA GCTCCTCCTG GACCATGGAG TGCGTGTGGA TGTGAAGGAC 
TGGGATGGCT GGGAGCCCCT GCATGCAGCT GCCTTCTGGG GACAGATGCA GATGGCAGAG 
CTATTGGTGT CCCATGGAGC TAGTCTCAGT GCAAGGACAT CCATGGATGA GATGCCAATA 
GACCTGTGCG AGGAGGAAGA GTTCAAGGTC CTGCTGCTGG AGCTAAAACA CAAGCATGAT 
GTGATCATGA AGTCACAGCT GAGGCACAAG TCATCCTTGA GCCGGAGGAC CTCCAGCGCA 
GGCAGCCGTG GGAAGGTGGT GCGGCGAGCC AGCCTGTCGG ACAGGACCAA CCTGTATAGG 
AAGGAGTATG AGGGAGAGGC CATCCTGTGG CAGCGGAGTG CAGCTGAGGA TCAGCGGACC 
TCCACCTACA ACGGGGACAT CAGGGAGACC AGGACAGACC AAGAGAATAA GGACCCTAAC 
CCCAGGCTGG AGAAGCCCGT GCTACTCTCC GAATTTCCTA CCAAGATCCC ACGAGGTGAA 
CTGGACATGC CTGTTGAGAA TGGCCTCCGG GCTCCGGTCA GTGCCTACCA GTATGCGCTG 
GCCAACGGGG ATGTCTGGAA GGTGCATGAG GTGCCTGACT ACAGCATGGC CTATGGCAAC 
CCTGGCGTGG CCGACGCCAC CCCGCCCTGG AGCAGCTACA AGGAACAGAG CCCTCAGACG 
CTTCTGGAGC TGAAGCGGCA GCGGGCTGCA GCCAAGCTGC TCAGCCACCC CTTCCTTAGC 
ACACACCTGG GCAGCAGCAT GGCCAGGACG GGCGAGAGTA GCAGTGAAGG CAAGGCCCCC 
TTGATCGGAG GCAGAACTTC ACCGTACAGC AGCAATGGGA CCTCGGTATA TTACACGGTC 
ACCAGCGGAG ATCCCCCACT CTTAAAGTTC AAGGCCCCCA TAGAGGAGAT GGAGGAGAAG 
GTGCATGGCT GTTGCCGTAT CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R16B ( NM_001172735.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166206.1 Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu32242
Clone ID Related Accession (Same CDS sequence)	NM_001172735.3 , NM_001172735.2
Accession Version	NM_001172735.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY125679.1, BC152467.1 and AB177855.1. On May 31, 2019 this sequence version replaced NM_001172735.2. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. This isoform (2) may undergo similar processing as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB177855.1, SRR1803614.233875.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2157511, SAMEA2159764 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGTC ACGTGGACCT GCTGACGGAG CTGCAGCTGC TGGAGAAGGT GCCCACGCTG 
GAGCGGCTGC GGGCTGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAATG GGCACAGTAC 
GAGCAGGACT TGCAGCACCG CAAGCGAAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGC 
CGCAAGAAAG TGTCCTTCGA GGCCAGCGTG GCCCTGCTGG AGGCCTCGCT GAGGAACGAC 
GCCGAGGAAG TACGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAATGAGGAC 
GGACTCACAG CCCTACACCA GTGCTGCATC GACAACTTTG AGGAAATTGT GAAGCTGCTC 
CTCTCCCATG GTGCCAATGT GAACGCCAAG GACAACGAGC TGTGGACACC TCTCCATGCT 
GCAGCCACCT GCGGCCACAT CAACCTGGTG AAGATCCTCG TTCAGTATGG GGCCGACTTG 
CTTGCTGTCA ACTCGGATGG GAACATGCCA TATGACCTCT GCGAGGATGA ACCCACCCTG 
GATGTCATCG AGACCTGCAT GGCATACCAG GGCATCACCC AAGAGAAAAT CAACGAGATG 
CGGGTGGCTC CTGAGCAGCA GATGATTGCG GACATCCACT GCATGATCGC AGCGGGCCAG 
GACCTGGACT GGATAGATGC CCAGGGTGCC ACACTGATGC AGATGGCAGA GCTATTGGTG 
TCCCATGGAG CTAGTCTCAG TGCAAGGACA TCCATGGATG AGATGCCAAT AGACCTGTGC 
GAGGAGGAAG AGTTCAAGGT CCTGCTGCTG GAGCTAAAAC ACAAGCATGA TGTGATCATG 
AAGTCACAGC TGAGGCACAA GTCATCCTTG AGCCGGAGGA CCTCCAGCGC AGGCAGCCGT 
GGGAAGGTGG TGCGGCGAGC CAGCCTGTCG GACAGGACCA ACCTGTATAG GAAGGAGTAT 
GAGGGAGAGG CCATCCTGTG GCAGCGGAGT GCAGCTGAGG ATCAGCGGAC CTCCACCTAC 
AACGGGGACA TCAGGGAGAC CAGGACAGAC CAAGAGAATA AGGACCCTAA CCCCAGGCTG 
GAGAAGCCCG TGCTACTCTC CGAATTTCCT ACCAAGATCC CACGAGGTGA ACTGGACATG 
CCTGTTGAGA ATGGCCTCCG GGCTCCGGTC AGTGCCTACC AGTATGCGCT GGCCAACGGG 
GATGTCTGGA AGGTGCATGA GGTGCCTGAC TACAGCATGG CCTATGGCAA CCCTGGCGTG 
GCCGACGCCA CCCCGCCCTG GAGCAGCTAC AAGGAACAGA GCCCTCAGAC GCTTCTGGAG 
CTGAAGCGGC AGCGGGCTGC AGCCAAGCTG CTCAGCCACC CCTTCCTTAG CACACACCTG 
GGCAGCAGCA TGGCCAGGAC GGGCGAGAGT AGCAGTGAAG GCAAGGCCCC CTTGATCGGA 
GGCAGAACTT CACCGTACAG CAGCAATGGG ACCTCGGTAT ATTACACGGT CACCAGCGGA 
GATCCCCCAC TCTTAAAGTT CAAGGCCCCC ATAGAGGAGA TGGAGGAGAA GGTGCATGGC 
TGTTGCCGTA TCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166206.1
CDS	198..1775
Translation	MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDLQHRKRKHERKRSTGGRRKKVSFEASVALLEASLRNDAEEVRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRVAPEQQMIADIHCMIAAGQDLDWIDAQGATLMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEVPDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMARTGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	NM_001172735.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172735.3

ORF Insert Sequence:

ATGGCCAGTC ACGTGGACCT GCTGACGGAG CTGCAGCTGC TGGAGAAGGT GCCCACGCTG 
GAGCGGCTGC GGGCTGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAATG GGCACAGTAC 
GAGCAGGACT TGCAGCACCG CAAGCGAAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGC 
CGCAAGAAAG TGTCCTTCGA GGCCAGCGTG GCCCTGCTGG AGGCCTCGCT GAGGAACGAC 
GCCGAGGAAG TACGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAATGAGGAC 
GGACTCACAG CCCTACACCA GTGCTGCATC GACAACTTTG AGGAAATTGT GAAGCTGCTC 
CTCTCCCATG GTGCCAATGT GAACGCCAAG GACAACGAGC TGTGGACACC TCTCCATGCT 
GCAGCCACCT GCGGCCACAT CAACCTGGTG AAGATCCTCG TTCAGTATGG GGCCGACTTG 
CTTGCTGTCA ACTCGGATGG GAACATGCCA TATGACCTCT GCGAGGATGA ACCCACCCTG 
GATGTCATCG AGACCTGCAT GGCATACCAG GGCATCACCC AAGAGAAAAT CAACGAGATG 
CGGGTGGCTC CTGAGCAGCA GATGATTGCG GACATCCACT GCATGATCGC AGCGGGCCAG 
GACCTGGACT GGATAGATGC CCAGGGTGCC ACACTGATGC AGATGGCAGA GCTATTGGTG 
TCCCATGGAG CTAGTCTCAG TGCAAGGACA TCCATGGATG AGATGCCAAT AGACCTGTGC 
GAGGAGGAAG AGTTCAAGGT CCTGCTGCTG GAGCTAAAAC ACAAGCATGA TGTGATCATG 
AAGTCACAGC TGAGGCACAA GTCATCCTTG AGCCGGAGGA CCTCCAGCGC AGGCAGCCGT 
GGGAAGGTGG TGCGGCGAGC CAGCCTGTCG GACAGGACCA ACCTGTATAG GAAGGAGTAT 
GAGGGAGAGG CCATCCTGTG GCAGCGGAGT GCAGCTGAGG ATCAGCGGAC CTCCACCTAC 
AACGGGGACA TCAGGGAGAC CAGGACAGAC CAAGAGAATA AGGACCCTAA CCCCAGGCTG 
GAGAAGCCCG TGCTACTCTC CGAATTTCCT ACCAAGATCC CACGAGGTGA ACTGGACATG 
CCTGTTGAGA ATGGCCTCCG GGCTCCGGTC AGTGCCTACC AGTATGCGCT GGCCAACGGG 
GATGTCTGGA AGGTGCATGA GGTGCCTGAC TACAGCATGG CCTATGGCAA CCCTGGCGTG 
GCCGACGCCA CCCCGCCCTG GAGCAGCTAC AAGGAACAGA GCCCTCAGAC GCTTCTGGAG 
CTGAAGCGGC AGCGGGCTGC AGCCAAGCTG CTCAGCCACC CCTTCCTTAG CACACACCTG 
GGCAGCAGCA TGGCCAGGAC GGGCGAGAGT AGCAGTGAAG GCAAGGCCCC CTTGATCGGA 
GGCAGAACTT CACCGTACAG CAGCAATGGG ACCTCGGTAT ATTACACGGT CACCAGCGGA 
GATCCCCCAC TCTTAAAGTT CAAGGCCCCC ATAGAGGAGA TGGAGGAGAA GGTGCATGGC 
TGTTGCCGTA TCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R16B ( NM_001172735.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166206.1 Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu32242
Clone ID Related Accession (Same CDS sequence)	NM_001172735.3 , NM_001172735.2
Accession Version	NM_001172735.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY125679.1, BC152467.1 and AB177855.1. On Sep 29, 2015 this sequence version replaced NM_001172735.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. This isoform (2) may undergo similar processing as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2157511, SAMEA2159764 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGTC ACGTGGACCT GCTGACGGAG CTGCAGCTGC TGGAGAAGGT GCCCACGCTG 
GAGCGGCTGC GGGCTGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAATG GGCACAGTAC 
GAGCAGGACT TGCAGCACCG CAAGCGAAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGC 
CGCAAGAAAG TGTCCTTCGA GGCCAGCGTG GCCCTGCTGG AGGCCTCGCT GAGGAACGAC 
GCCGAGGAAG TACGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAATGAGGAC 
GGACTCACAG CCCTACACCA GTGCTGCATC GACAACTTTG AGGAAATTGT GAAGCTGCTC 
CTCTCCCATG GTGCCAATGT GAACGCCAAG GACAACGAGC TGTGGACACC TCTCCATGCT 
GCAGCCACCT GCGGCCACAT CAACCTGGTG AAGATCCTCG TTCAGTATGG GGCCGACTTG 
CTTGCTGTCA ACTCGGATGG GAACATGCCA TATGACCTCT GCGAGGATGA ACCCACCCTG 
GATGTCATCG AGACCTGCAT GGCATACCAG GGCATCACCC AAGAGAAAAT CAACGAGATG 
CGGGTGGCTC CTGAGCAGCA GATGATTGCG GACATCCACT GCATGATCGC AGCGGGCCAG 
GACCTGGACT GGATAGATGC CCAGGGTGCC ACACTGATGC AGATGGCAGA GCTATTGGTG 
TCCCATGGAG CTAGTCTCAG TGCAAGGACA TCCATGGATG AGATGCCAAT AGACCTGTGC 
GAGGAGGAAG AGTTCAAGGT CCTGCTGCTG GAGCTAAAAC ACAAGCATGA TGTGATCATG 
AAGTCACAGC TGAGGCACAA GTCATCCTTG AGCCGGAGGA CCTCCAGCGC AGGCAGCCGT 
GGGAAGGTGG TGCGGCGAGC CAGCCTGTCG GACAGGACCA ACCTGTATAG GAAGGAGTAT 
GAGGGAGAGG CCATCCTGTG GCAGCGGAGT GCAGCTGAGG ATCAGCGGAC CTCCACCTAC 
AACGGGGACA TCAGGGAGAC CAGGACAGAC CAAGAGAATA AGGACCCTAA CCCCAGGCTG 
GAGAAGCCCG TGCTACTCTC CGAATTTCCT ACCAAGATCC CACGAGGTGA ACTGGACATG 
CCTGTTGAGA ATGGCCTCCG GGCTCCGGTC AGTGCCTACC AGTATGCGCT GGCCAACGGG 
GATGTCTGGA AGGTGCATGA GGTGCCTGAC TACAGCATGG CCTATGGCAA CCCTGGCGTG 
GCCGACGCCA CCCCGCCCTG GAGCAGCTAC AAGGAACAGA GCCCTCAGAC GCTTCTGGAG 
CTGAAGCGGC AGCGGGCTGC AGCCAAGCTG CTCAGCCACC CCTTCCTTAG CACACACCTG 
GGCAGCAGCA TGGCCAGGAC GGGCGAGAGT AGCAGTGAAG GCAAGGCCCC CTTGATCGGA 
GGCAGAACTT CACCGTACAG CAGCAATGGG ACCTCGGTAT ATTACACGGT CACCAGCGGA 
GATCCCCCAC TCTTAAAGTT CAAGGCCCCC ATAGAGGAGA TGGAGGAGAA GGTGCATGGC 
TGTTGCCGTA TCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166206.1
CDS	202..1779
Translation	MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDLQHRKRKHERKRSTGGRRKKVSFEASVALLEASLRNDAEEVRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRVAPEQQMIADIHCMIAAGQDLDWIDAQGATLMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEVPDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMARTGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	NM_001172735.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172735.2

ORF Insert Sequence:

ATGGCCAGTC ACGTGGACCT GCTGACGGAG CTGCAGCTGC TGGAGAAGGT GCCCACGCTG 
GAGCGGCTGC GGGCTGCCCA GAAGCGCCGG GCCCAGCAGC TGAAGAAATG GGCACAGTAC 
GAGCAGGACT TGCAGCACCG CAAGCGAAAG CATGAGCGGA AGCGCAGCAC GGGCGGCCGC 
CGCAAGAAAG TGTCCTTCGA GGCCAGCGTG GCCCTGCTGG AGGCCTCGCT GAGGAACGAC 
GCCGAGGAAG TACGCTACTT CCTGAAGAAT AAGGTCAGCC CTGATTTGTG CAATGAGGAC 
GGACTCACAG CCCTACACCA GTGCTGCATC GACAACTTTG AGGAAATTGT GAAGCTGCTC 
CTCTCCCATG GTGCCAATGT GAACGCCAAG GACAACGAGC TGTGGACACC TCTCCATGCT 
GCAGCCACCT GCGGCCACAT CAACCTGGTG AAGATCCTCG TTCAGTATGG GGCCGACTTG 
CTTGCTGTCA ACTCGGATGG GAACATGCCA TATGACCTCT GCGAGGATGA ACCCACCCTG 
GATGTCATCG AGACCTGCAT GGCATACCAG GGCATCACCC AAGAGAAAAT CAACGAGATG 
CGGGTGGCTC CTGAGCAGCA GATGATTGCG GACATCCACT GCATGATCGC AGCGGGCCAG 
GACCTGGACT GGATAGATGC CCAGGGTGCC ACACTGATGC AGATGGCAGA GCTATTGGTG 
TCCCATGGAG CTAGTCTCAG TGCAAGGACA TCCATGGATG AGATGCCAAT AGACCTGTGC 
GAGGAGGAAG AGTTCAAGGT CCTGCTGCTG GAGCTAAAAC ACAAGCATGA TGTGATCATG 
AAGTCACAGC TGAGGCACAA GTCATCCTTG AGCCGGAGGA CCTCCAGCGC AGGCAGCCGT 
GGGAAGGTGG TGCGGCGAGC CAGCCTGTCG GACAGGACCA ACCTGTATAG GAAGGAGTAT 
GAGGGAGAGG CCATCCTGTG GCAGCGGAGT GCAGCTGAGG ATCAGCGGAC CTCCACCTAC 
AACGGGGACA TCAGGGAGAC CAGGACAGAC CAAGAGAATA AGGACCCTAA CCCCAGGCTG 
GAGAAGCCCG TGCTACTCTC CGAATTTCCT ACCAAGATCC CACGAGGTGA ACTGGACATG 
CCTGTTGAGA ATGGCCTCCG GGCTCCGGTC AGTGCCTACC AGTATGCGCT GGCCAACGGG 
GATGTCTGGA AGGTGCATGA GGTGCCTGAC TACAGCATGG CCTATGGCAA CCCTGGCGTG 
GCCGACGCCA CCCCGCCCTG GAGCAGCTAC AAGGAACAGA GCCCTCAGAC GCTTCTGGAG 
CTGAAGCGGC AGCGGGCTGC AGCCAAGCTG CTCAGCCACC CCTTCCTTAG CACACACCTG 
GGCAGCAGCA TGGCCAGGAC GGGCGAGAGT AGCAGTGAAG GCAAGGCCCC CTTGATCGGA 
GGCAGAACTT CACCGTACAG CAGCAATGGG ACCTCGGTAT ATTACACGGT CACCAGCGGA 
GATCCCCCAC TCTTAAAGTT CAAGGCCCCC ATAGAGGAGA TGGAGGAGAA GGTGCATGGC 
TGTTGCCGTA TCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R16B ( XM_011528768.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527070.1 Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.

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CloneID	OHu66753
Clone ID Related Accession (Same CDS sequence)	XM_011528768.3 , XM_011528768.2
Accession Version	XM_011528768.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011528768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTAA CCATCTCGAG GTCAGGAAGA TGCTCTGTGA GACGGAGTGG GTCAGATGCC 
AAAGAAAACA CAAGCTTGTC AGCGGTGATG TCTCCCTGCA AAGGCAGCCG CCACCAGCTT 
CCTGCCGAGA TGGGCCGGCT CAGCCTCACT TGCTACAGCT GGAGATGGAG AGGACCAGAT 
GGCCCTGTTT GGCCCAGATT CCAGCCAGAT TGCCAGGGAG GTGTAGGCGG AGCAGCGGGG 
TCGGCGCCAG GAGCAGGGAT ACTACGCTAC TTCCTGAAGA ATAAGGTCAG CCCTGATTTG 
TGCAATGAGG ACGGACTCAC AGCCCTACAC CAGTGCTGCA TCGACAACTT TGAGGAAATT 
GTGAAGCTGC TCCTCTCCCA TGGTGCCAAT GTGAACGCCA AGGACAACGA GCTGTGGACA 
CCTCTCCATG CTGCAGCCAC CTGCGGCCAC ATCAACCTGG TGAAGATCCT CGTTCAGTAT 
GGGGCCGACT TGCTTGCTGT CAACTCGGAT GGGAACATGC CATATGACCT CTGCGAGGAT 
GAACCCACCC TGGATGTCAT CGAGACCTGC ATGGCATACC AGGGCATCAC CCAAGAGAAA 
ATCAACGAGA TGCGGGTGGC TCCTGAGCAG CAGATGATTG CGGACATCCA CTGCATGATC 
GCAGCGGGCC AGGACCTGGA CTGGATAGAT GCCCAGGGTG CCACACTGCT GCACATAGCT 
GGAGCCAATG GATACCTGCG GGCAGCTGAG CTCCTCCTGG ACCATGGAGT GCGTGTGGAT 
GTGAAGGACT GGGATGGCTG GGAGCCCCTG CATGCAGCTG CCTTCTGGGG ACAGATGCAG 
ATGGCAGAGC TATTGGTGTC CCATGGAGCT AGTCTCAGTG CAAGGACATC CATGGATGAG 
ATGCCAATAG ACCTGTGCGA GGAGGAAGAG TTCAAGGTCC TGCTGCTGGA GCTAAAACAC 
AAGCATGATG TGATCATGAA GTCACAGCTG AGGCACAAGT CATCCTTGAG CCGGAGGACC 
TCCAGCGCAG GCAGCCGTGG GAAGGTGGTG CGGCGAGCCA GCCTGTCGGA CAGGACCAAC 
CTGTATAGGA AGGAGTATGA GGGAGAGGCC ATCCTGTGGC AGCGGAGTGC AGCTGAGGAT 
CAGCGGACCT CCACCTACAA CGGGGACATC AGGGAGACCA GGACAGACCA AGAGAATAAG 
GACCCTAACC CCAGGCTGGA GAAGCCCGTG CTACTCTCCG AATTTCCTAC CAAGATCCCA 
CGAGGTGAAC TGGACATGCC TGTTGAGAAT GGCCTCCGGG CTCCGGTCAG TGCCTACCAG 
TATGCGCTGG CCAACGGGGA TGTCTGGAAG GTGCATGAGG TGCCTGACTA CAGCATGGCC 
TATGGCAACC CTGGCGTGGC CGACGCCACC CCGCCCTGGA GCAGCTACAA GGAACAGAGC 
CCTCAGACGC TTCTGGAGCT GAAGCGGCAG CGGGCTGCAG CCAAGCTGCT CAGCCACCCC 
TTCCTTAGCA CACACCTGGG CAGCAGCATG GCCAGGACGG GCGAGAGTAG CAGTGAAGGC 
AAGGCCCCCT TGATCGGAGG CAGAACTTCA CCGTACAGCA GCAATGGGAC CTCGGTATAT 
TACACGGTCA CCAGCGGAGA TCCCCCACTC TTAAAGTTCA AGGCCCCCAT AGAGGAGATG 
GAGGAGAAGG TGCATGGCTG TTGCCGTATC TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527070.1
CDS	756..2471
Translation	MLLTISRSGRCSVRRSGSDAKENTSLSAVMSPCKGSRHQLPAEMGRLSLTCYSWRWRGPDGPVWPRFQPDCQGGVGGAAGSAPGAGILRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRVAPEQQMIADIHCMIAAGQDLDWIDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEVPDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMARTGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	XM_011528768.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528768.3

ORF Insert Sequence:

ATGTTGCTAA CCATCTCGAG GTCAGGAAGA TGCTCTGTGA GACGGAGTGG GTCAGATGCC 
AAAGAAAACA CAAGCTTGTC AGCGGTGATG TCTCCCTGCA AAGGCAGCCG CCACCAGCTT 
CCTGCCGAGA TGGGCCGGCT CAGCCTCACT TGCTACAGCT GGAGATGGAG AGGACCAGAT 
GGCCCTGTTT GGCCCAGATT CCAGCCAGAT TGCCAGGGAG GTGTAGGCGG AGCAGCGGGG 
TCGGCGCCAG GAGCAGGGAT ACTACGCTAC TTCCTGAAGA ATAAGGTCAG CCCTGATTTG 
TGCAATGAGG ACGGACTCAC AGCCCTACAC CAGTGCTGCA TCGACAACTT TGAGGAAATT 
GTGAAGCTGC TCCTCTCCCA TGGTGCCAAT GTGAACGCCA AGGACAACGA GCTGTGGACA 
CCTCTCCATG CTGCAGCCAC CTGCGGCCAC ATCAACCTGG TGAAGATCCT CGTTCAGTAT 
GGGGCCGACT TGCTTGCTGT CAACTCGGAT GGGAACATGC CATATGACCT CTGCGAGGAT 
GAACCCACCC TGGATGTCAT CGAGACCTGC ATGGCATACC AGGGCATCAC CCAAGAGAAA 
ATCAACGAGA TGCGGGTGGC TCCTGAGCAG CAGATGATTG CGGACATCCA CTGCATGATC 
GCAGCGGGCC AGGACCTGGA CTGGATAGAT GCCCAGGGTG CCACACTGCT GCACATAGCT 
GGAGCCAATG GATACCTGCG GGCAGCTGAG CTCCTCCTGG ACCATGGAGT GCGTGTGGAT 
GTGAAGGACT GGGATGGCTG GGAGCCCCTG CATGCAGCTG CCTTCTGGGG ACAGATGCAG 
ATGGCAGAGC TATTGGTGTC CCATGGAGCT AGTCTCAGTG CAAGGACATC CATGGATGAG 
ATGCCAATAG ACCTGTGCGA GGAGGAAGAG TTCAAGGTCC TGCTGCTGGA GCTAAAACAC 
AAGCATGATG TGATCATGAA GTCACAGCTG AGGCACAAGT CATCCTTGAG CCGGAGGACC 
TCCAGCGCAG GCAGCCGTGG GAAGGTGGTG CGGCGAGCCA GCCTGTCGGA CAGGACCAAC 
CTGTATAGGA AGGAGTATGA GGGAGAGGCC ATCCTGTGGC AGCGGAGTGC AGCTGAGGAT 
CAGCGGACCT CCACCTACAA CGGGGACATC AGGGAGACCA GGACAGACCA AGAGAATAAG 
GACCCTAACC CCAGGCTGGA GAAGCCCGTG CTACTCTCCG AATTTCCTAC CAAGATCCCA 
CGAGGTGAAC TGGACATGCC TGTTGAGAAT GGCCTCCGGG CTCCGGTCAG TGCCTACCAG 
TATGCGCTGG CCAACGGGGA TGTCTGGAAG GTGCATGAGG TGCCTGACTA CAGCATGGCC 
TATGGCAACC CTGGCGTGGC CGACGCCACC CCGCCCTGGA GCAGCTACAA GGAACAGAGC 
CCTCAGACGC TTCTGGAGCT GAAGCGGCAG CGGGCTGCAG CCAAGCTGCT CAGCCACCCC 
TTCCTTAGCA CACACCTGGG CAGCAGCATG GCCAGGACGG GCGAGAGTAG CAGTGAAGGC 
AAGGCCCCCT TGATCGGAGG CAGAACTTCA CCGTACAGCA GCAATGGGAC CTCGGTATAT 
TACACGGTCA CCAGCGGAGA TCCCCCACTC TTAAAGTTCA AGGCCCCCAT AGAGGAGATG 
GAGGAGAAGG TGCATGGCTG TTGCCGTATC TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R16B ( XM_011528768.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527070.1 Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu66753
Clone ID Related Accession (Same CDS sequence)	XM_011528768.3 , XM_011528768.2
Accession Version	XM_011528768.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory inhibitor subunit 16B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTAA CCATCTCGAG GTCAGGAAGA TGCTCTGTGA GACGGAGTGG GTCAGATGCC 
AAAGAAAACA CAAGCTTGTC AGCGGTGATG TCTCCCTGCA AAGGCAGCCG CCACCAGCTT 
CCTGCCGAGA TGGGCCGGCT CAGCCTCACT TGCTACAGCT GGAGATGGAG AGGACCAGAT 
GGCCCTGTTT GGCCCAGATT CCAGCCAGAT TGCCAGGGAG GTGTAGGCGG AGCAGCGGGG 
TCGGCGCCAG GAGCAGGGAT ACTACGCTAC TTCCTGAAGA ATAAGGTCAG CCCTGATTTG 
TGCAATGAGG ACGGACTCAC AGCCCTACAC CAGTGCTGCA TCGACAACTT TGAGGAAATT 
GTGAAGCTGC TCCTCTCCCA TGGTGCCAAT GTGAACGCCA AGGACAACGA GCTGTGGACA 
CCTCTCCATG CTGCAGCCAC CTGCGGCCAC ATCAACCTGG TGAAGATCCT CGTTCAGTAT 
GGGGCCGACT TGCTTGCTGT CAACTCGGAT GGGAACATGC CATATGACCT CTGCGAGGAT 
GAACCCACCC TGGATGTCAT CGAGACCTGC ATGGCATACC AGGGCATCAC CCAAGAGAAA 
ATCAACGAGA TGCGGGTGGC TCCTGAGCAG CAGATGATTG CGGACATCCA CTGCATGATC 
GCAGCGGGCC AGGACCTGGA CTGGATAGAT GCCCAGGGTG CCACACTGCT GCACATAGCT 
GGAGCCAATG GATACCTGCG GGCAGCTGAG CTCCTCCTGG ACCATGGAGT GCGTGTGGAT 
GTGAAGGACT GGGATGGCTG GGAGCCCCTG CATGCAGCTG CCTTCTGGGG ACAGATGCAG 
ATGGCAGAGC TATTGGTGTC CCATGGAGCT AGTCTCAGTG CAAGGACATC CATGGATGAG 
ATGCCAATAG ACCTGTGCGA GGAGGAAGAG TTCAAGGTCC TGCTGCTGGA GCTAAAACAC 
AAGCATGATG TGATCATGAA GTCACAGCTG AGGCACAAGT CATCCTTGAG CCGGAGGACC 
TCCAGCGCAG GCAGCCGTGG GAAGGTGGTG CGGCGAGCCA GCCTGTCGGA CAGGACCAAC 
CTGTATAGGA AGGAGTATGA GGGAGAGGCC ATCCTGTGGC AGCGGAGTGC AGCTGAGGAT 
CAGCGGACCT CCACCTACAA CGGGGACATC AGGGAGACCA GGACAGACCA AGAGAATAAG 
GACCCTAACC CCAGGCTGGA GAAGCCCGTG CTACTCTCCG AATTTCCTAC CAAGATCCCA 
CGAGGTGAAC TGGACATGCC TGTTGAGAAT GGCCTCCGGG CTCCGGTCAG TGCCTACCAG 
TATGCGCTGG CCAACGGGGA TGTCTGGAAG GTGCATGAGG TGCCTGACTA CAGCATGGCC 
TATGGCAACC CTGGCGTGGC CGACGCCACC CCGCCCTGGA GCAGCTACAA GGAACAGAGC 
CCTCAGACGC TTCTGGAGCT GAAGCGGCAG CGGGCTGCAG CCAAGCTGCT CAGCCACCCC 
TTCCTTAGCA CACACCTGGG CAGCAGCATG GCCAGGACGG GCGAGAGTAG CAGTGAAGGC 
AAGGCCCCCT TGATCGGAGG CAGAACTTCA CCGTACAGCA GCAATGGGAC CTCGGTATAT 
TACACGGTCA CCAGCGGAGA TCCCCCACTC TTAAAGTTCA AGGCCCCCAT AGAGGAGATG 
GAGGAGAAGG TGCATGGCTG TTGCCGTATC TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527070.1
CDS	753..2468
Translation	MLLTISRSGRCSVRRSGSDAKENTSLSAVMSPCKGSRHQLPAEMGRLSLTCYSWRWRGPDGPVWPRFQPDCQGGVGGAAGSAPGAGILRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHAAATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRVAPEQQMIADIHCMIAAGQDLDWIDAQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMQMAELLVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRASLSDRTNLYRKEYEGEAILWQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFPTKIPRGELDMPVENGLRAPVSAYQYALANGDVWKVHEVPDYSMAYGNPGVADATPPWSSYKEQSPQTLLELKRQRAAAKLLSHPFLSTHLGSSMARTGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPIEEMEEKVHGCCRIS

Target ORF information:

RefSeq Version	XM_011528768.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 16B (PPP1R16B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528768.2

ORF Insert Sequence:

ATGTTGCTAA CCATCTCGAG GTCAGGAAGA TGCTCTGTGA GACGGAGTGG GTCAGATGCC 
AAAGAAAACA CAAGCTTGTC AGCGGTGATG TCTCCCTGCA AAGGCAGCCG CCACCAGCTT 
CCTGCCGAGA TGGGCCGGCT CAGCCTCACT TGCTACAGCT GGAGATGGAG AGGACCAGAT 
GGCCCTGTTT GGCCCAGATT CCAGCCAGAT TGCCAGGGAG GTGTAGGCGG AGCAGCGGGG 
TCGGCGCCAG GAGCAGGGAT ACTACGCTAC TTCCTGAAGA ATAAGGTCAG CCCTGATTTG 
TGCAATGAGG ACGGACTCAC AGCCCTACAC CAGTGCTGCA TCGACAACTT TGAGGAAATT 
GTGAAGCTGC TCCTCTCCCA TGGTGCCAAT GTGAACGCCA AGGACAACGA GCTGTGGACA 
CCTCTCCATG CTGCAGCCAC CTGCGGCCAC ATCAACCTGG TGAAGATCCT CGTTCAGTAT 
GGGGCCGACT TGCTTGCTGT CAACTCGGAT GGGAACATGC CATATGACCT CTGCGAGGAT 
GAACCCACCC TGGATGTCAT CGAGACCTGC ATGGCATACC AGGGCATCAC CCAAGAGAAA 
ATCAACGAGA TGCGGGTGGC TCCTGAGCAG CAGATGATTG CGGACATCCA CTGCATGATC 
GCAGCGGGCC AGGACCTGGA CTGGATAGAT GCCCAGGGTG CCACACTGCT GCACATAGCT 
GGAGCCAATG GATACCTGCG GGCAGCTGAG CTCCTCCTGG ACCATGGAGT GCGTGTGGAT 
GTGAAGGACT GGGATGGCTG GGAGCCCCTG CATGCAGCTG CCTTCTGGGG ACAGATGCAG 
ATGGCAGAGC TATTGGTGTC CCATGGAGCT AGTCTCAGTG CAAGGACATC CATGGATGAG 
ATGCCAATAG ACCTGTGCGA GGAGGAAGAG TTCAAGGTCC TGCTGCTGGA GCTAAAACAC 
AAGCATGATG TGATCATGAA GTCACAGCTG AGGCACAAGT CATCCTTGAG CCGGAGGACC 
TCCAGCGCAG GCAGCCGTGG GAAGGTGGTG CGGCGAGCCA GCCTGTCGGA CAGGACCAAC 
CTGTATAGGA AGGAGTATGA GGGAGAGGCC ATCCTGTGGC AGCGGAGTGC AGCTGAGGAT 
CAGCGGACCT CCACCTACAA CGGGGACATC AGGGAGACCA GGACAGACCA AGAGAATAAG 
GACCCTAACC CCAGGCTGGA GAAGCCCGTG CTACTCTCCG AATTTCCTAC CAAGATCCCA 
CGAGGTGAAC TGGACATGCC TGTTGAGAAT GGCCTCCGGG CTCCGGTCAG TGCCTACCAG 
TATGCGCTGG CCAACGGGGA TGTCTGGAAG GTGCATGAGG TGCCTGACTA CAGCATGGCC 
TATGGCAACC CTGGCGTGGC CGACGCCACC CCGCCCTGGA GCAGCTACAA GGAACAGAGC 
CCTCAGACGC TTCTGGAGCT GAAGCGGCAG CGGGCTGCAG CCAAGCTGCT CAGCCACCCC 
TTCCTTAGCA CACACCTGGG CAGCAGCATG GCCAGGACGG GCGAGAGTAG CAGTGAAGGC 
AAGGCCCCCT TGATCGGAGG CAGAACTTCA CCGTACAGCA GCAATGGGAC CTCGGTATAT 
TACACGGTCA CCAGCGGAGA TCCCCCACTC TTAAAGTTCA AGGCCCCCAT AGAGGAGATG 
GAGGAGAAGG TGCATGGCTG TTGCCGTATC TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TIMAP, the versatile protein phosphatase 1 regulator in endothelial cells.
IUBMB life69(12)918-928(2017 Dec)
Boratk? A,Csortos C

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Cell systems4(4)430-444.e5(2017 Apr)
Yadav L,Tamene F,G??s H,van Drogen A,Katainen R,Aebersold R,Gstaiger M,Varjosalo M

PKC mediated phosphorylation of TIMAP regulates PP1c activity and endothelial barrier function.
Biochimica et biophysica acta1864(2)431-439(2017 02)
Boratk? A,Csortos C

Regulation of merlin by protein phosphatase 1-TIMAP and EBP50 in endothelial cells.
The international journal of biochemistry & cell biology8210-17(2017 01)
Boratk? A,P?ter M,Csortos C

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PPP1R16B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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