B4GALT1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	B4GALT1
Entrez Gene ID	281781
Full Name	beta-1,4-galactosyltransferase 1
Synonyms	GGTB2
General protein information	Preferred Names beta-1,4-galactosyltransferase 1 Names beta-1,4-galactosyltransferase 1 UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1 UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1 b4Gal-T1 beta-1,4-GalTase 1 beta-galactosyltransferase 2 (glycoprotein) beta4Gal-T1 glycoprotein-4-beta-galactosyltransferase 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_177512.2	NP_803478.1	beta-1,4-galactosyltransferase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	B4GALT1	XP_003312085.1
Bos taurus (cattle)	B4GALT1	NP_803478.1
Rattus norvegicus (Norway rat)	B4galt1	NP_445739.1
Gallus gallus (chicken)	B4GALT1	NP_990533.1
Mus musculus (house mouse)	B4galt1	NP_071641.1
Danio rerio (zebrafish)	zgc:154116	NP_001070727.2
Xenopus tropicalis (tropical clawed frog)	b4galt1.2	NP_001016664.1
Homo sapiens (human)	B4GALT1	NP_001488.2
	B4GALT1	XP_001100938.1
Canis lupus familiaris (dog)	B4GALT1	XP_538701.3
Xenopus tropicalis (tropical clawed frog)	b4galt1.1	NP_001120432.1

Gene Ontology
Bibliography

Related articles in PubMed

Engineering ?1,4-galactosyltransferase I to reduce secretion and enhance N-glycan elongation in insect cells.
Geisler C, Mabashi-Asazuma H, Kuo CW, Khoo KH, Jarvis DL
Journal of biotechnology19352-65(2015 Jan)

Array-assisted characterization of a fucosyltransferase required for the biosynthesis of complex core modifications of nematode N-glycans.
Yan S, Serna S, Reichardt NC, Paschinger K, Wilson IB
The Journal of biological chemistry288(29)21015-28(2013 Jul)

Association of polymorphism of the ?(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins.
Shahbazkia HR, Aminlari M, Cravador A
Molecular biology reports39(6)6715-21(2012 Jun)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Mutation of arginine 228 to lysine enhances the glucosyltransferase activity of bovine beta-1,4-galactosyltransferase I.
Ramakrishnan B, Boeggeman E, Qasba PK
Biochemistry44(9)3202-10(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hypothesized that engineering B4GALT1 to block cleavage and secretion would enhance its retention and its function; to test this hypothesis, replaced CTS domains of bovine B4GALT1 with those from human FUT7, which is not cleaved and secreted.
Title: Engineering ?1,4-galactosyltransferase I to reduce secretion and enhance N-glycan elongation in insect cells.

the beta4galt1 gene is potentially associated with milk production traits in dairy cows and should be considered for further studies on genetics of the milk production traits.
Title: Association of polymorphism of the ?(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins.

Crystal structure of mutant Met344His beta4Gal-T1 defined at 2.3 angstrom resolution reveals that the N-acetylglucosamine residue at nonreducing end of chitobiose makes extensive hydrophobic interactions with highly conserved tyrosine-286 of beta4Gal-T1.
Title: Effect of the Met344His mutation on the conformational dynamics of bovine beta-1,4-galactosyltransferase: crystal structure of the Met344His mutant in complex with chitobiose.

The following B4GALT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B4GALT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41333	NM_177512.2 Latest version!	Bos taurus beta-1,4-galactosyltransferase 1 (B4GALT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B4GALT1 ( NM_177512.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_803478.1 Bos taurus beta-1,4-galactosyltransferase 1 (B4GALT1), mRNA.

Price & Availability↑

CloneID	OBa41333
Clone ID Related Accession (Same CDS sequence)	NM_177512.2
Accession Version	NM_177512.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	beta-1,4-galactosyltransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X14558.1. On or before Mar 29, 2005 this sequence version replaced XM_612587.1, NM_177512.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X14558.1, BC120415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN05861888 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTTTC GGGAGCCGCT CCTGGGCGGC AGTGCCGCGA TGCCGGGCGC GTCCCTGCAG 
CGGGCCTGCC GCCTCCTCGT GGCTGTCTGC GCCCTGCATC TTGGCGTCAC CCTCGTCTAT 
TACCTGGCCG GCCGAGACCT AAGACGCCTG CCTCAGCTGG TCGGAGTCCA CCCACCGCTT 
CAGGGAAGCT CTCACGGCGC CGCCGCTATC GGGCAGCCCT CCGGGGAGCT CCGGCTGCGA 
GGGGTCGCAC CGCCGCCGCC TTTGCAGAAC TCTTCCAAGC CGCGCTCGCG GGCCCCCTCC 
AACCTAGACG CGTACTCTCA CCCCGGCCCT GGCCCGGGCC CGGGGAGCAA CTTGACTTCG 
GCCCCAGTGC CCTCCACCAC AACACGCTCG CTGACCGCAT GCCCTGAGGA GTCCCCGCTG 
CTCGTCGGCC CCATGCTGAT TGAGTTTAAC ATACCTGTGG ACCTGAAGCT TATCGAGCAG 
CAGAACCCGA AGGTGAAGTT GGGTGGTCGC TACACCCCCA TGGACTGCAT CTCTCCTCAC 
AAGGTGGCCA TCATCATTCT ATTCCGCAAC CGGCAGGAAC ACCTCAAGTA CTGGCTGTAT 
TACTTGCACC CAATGGTACA GCGTCAGCAG TTAGACTATG GCATCTATGT TATCAACCAG 
GCTGGAGAGT CCATGTTCAA CCGCGCAAAG CTCCTCAATG TTGGCTTTAA AGAGGCCTTG 
AAGGACTATG ACTACAACTG CTTTGTGTTT AGCGATGTGG ACCTCATCCC AATGAACGAC 
CATAACACCT ACAGGTGCTT TTCACAGCCA CGGCACATTT CTGTAGCAAT GGATAAGTTT 
GGATTTAGCC TACCTTACGT GCAGTATTTT GGAGGTGTCT CTGCTCTAAG TAAACAACAG 
TTTCTCAGCA TCAATGGATT TCCTAATAAC TACTGGGGCT GGGGAGGTGA AGATGATGAC 
ATTTATAACA GATTAGCTTT TAGAGGCATG TCTGTGTCTC GCCCAAATGC TGTGATCGGG 
AAGTGTCGGA TGATCCGCCA CTCGAGAGAC AAGAAAAATG AACCTAATCC TCAGAGGTTT 
GACCGAATTG CACATACAAA GGAGACAATG CTCTCTGATG GTTTGAACTC ACTCACCTAC 
ATGGTGTTAG AGGTCCAGAG GTACCCGTTG TATACCAAAA TCACAGTGGA CATCGGGACG 
CCGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_803478.1
CDS	184..1392
Translation	MKFREPLLGGSAAMPGASLQRACRLLVAVCALHLGVTLVYYLAGRDLRRLPQLVGVHPPLQGSSHGAAAIGQPSGELRLRGVAPPPPLQNSSKPRSRAPSNLDAYSHPGPGPGPGSNLTSAPVPSTTTRSLTACPEESPLLVGPMLIEFNIPVDLKLIEQQNPKVKLGGRYTPMDCISPHKVAIIILFRNRQEHLKYWLYYLHPMVQRQQLDYGIYVINQAGESMFNRAKLLNVGFKEALKDYDYNCFVFSDVDLIPMNDHNTYRCFSQPRHISVAMDKFGFSLPYVQYFGGVSALSKQQFLSINGFPNNYWGWGGEDDDIYNRLAFRGMSVSRPNAVIGKCRMIRHSRDKKNEPNPQRFDRIAHTKETMLSDGLNSLTYMVLEVQRYPLYTKITVDIGTPS

Target ORF information:

RefSeq Version	NM_177512.2
Organism	Bos taurus(cattle)
Definition	Bos taurus beta-1,4-galactosyltransferase 1 (B4GALT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177512.2

ORF Insert Sequence:

ATGAAGTTTC GGGAGCCGCT CCTGGGCGGC AGTGCCGCGA TGCCGGGCGC GTCCCTGCAG 
CGGGCCTGCC GCCTCCTCGT GGCTGTCTGC GCCCTGCATC TTGGCGTCAC CCTCGTCTAT 
TACCTGGCCG GCCGAGACCT AAGACGCCTG CCTCAGCTGG TCGGAGTCCA CCCACCGCTT 
CAGGGAAGCT CTCACGGCGC CGCCGCTATC GGGCAGCCCT CCGGGGAGCT CCGGCTGCGA 
GGGGTCGCAC CGCCGCCGCC TTTGCAGAAC TCTTCCAAGC CGCGCTCGCG GGCCCCCTCC 
AACCTAGACG CGTACTCTCA CCCCGGCCCT GGCCCGGGCC CGGGGAGCAA CTTGACTTCG 
GCCCCAGTGC CCTCCACCAC AACACGCTCG CTGACCGCAT GCCCTGAGGA GTCCCCGCTG 
CTCGTCGGCC CCATGCTGAT TGAGTTTAAC ATACCTGTGG ACCTGAAGCT TATCGAGCAG 
CAGAACCCGA AGGTGAAGTT GGGTGGTCGC TACACCCCCA TGGACTGCAT CTCTCCTCAC 
AAGGTGGCCA TCATCATTCT ATTCCGCAAC CGGCAGGAAC ACCTCAAGTA CTGGCTGTAT 
TACTTGCACC CAATGGTACA GCGTCAGCAG TTAGACTATG GCATCTATGT TATCAACCAG 
GCTGGAGAGT CCATGTTCAA CCGCGCAAAG CTCCTCAATG TTGGCTTTAA AGAGGCCTTG 
AAGGACTATG ACTACAACTG CTTTGTGTTT AGCGATGTGG ACCTCATCCC AATGAACGAC 
CATAACACCT ACAGGTGCTT TTCACAGCCA CGGCACATTT CTGTAGCAAT GGATAAGTTT 
GGATTTAGCC TACCTTACGT GCAGTATTTT GGAGGTGTCT CTGCTCTAAG TAAACAACAG 
TTTCTCAGCA TCAATGGATT TCCTAATAAC TACTGGGGCT GGGGAGGTGA AGATGATGAC 
ATTTATAACA GATTAGCTTT TAGAGGCATG TCTGTGTCTC GCCCAAATGC TGTGATCGGG 
AAGTGTCGGA TGATCCGCCA CTCGAGAGAC AAGAAAAATG AACCTAATCC TCAGAGGTTT 
GACCGAATTG CACATACAAA GGAGACAATG CTCTCTGATG GTTTGAACTC ACTCACCTAC 
ATGGTGTTAG AGGTCCAGAG GTACCCGTTG TATACCAAAA TCACAGTGGA CATCGGGACG 
CCGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Engineering ?1,4-galactosyltransferase I to reduce secretion and enhance N-glycan elongation in insect cells.
Journal of biotechnology19352-65(2015 Jan)
Geisler C,Mabashi-Asazuma H,Kuo CW,Khoo KH,Jarvis DL

Array-assisted characterization of a fucosyltransferase required for the biosynthesis of complex core modifications of nematode N-glycans.
The Journal of biological chemistry288(29)21015-28(2013 Jul)
Yan S,Serna S,Reichardt NC,Paschinger K,Wilson IB

Association of polymorphism of the ?(1, 4)-galactosyltransferase-I gene with milk production traits in Holsteins.
Molecular biology reports39(6)6715-21(2012 Jun)
Shahbazkia HR,Aminlari M,Cravador A

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Mutation of arginine 228 to lysine enhances the glucosyltransferase activity of bovine beta-1,4-galactosyltransferase I.
Biochemistry44(9)3202-10(2005 Mar)
Ramakrishnan B,Boeggeman E,Qasba PK

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B4GALT1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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