TLR2 cDNA ORF clone, Bos taurus(cattle)

The following TLR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa01524 NM_174197.2
Latest version!
Bos taurus toll like receptor 2 (TLR2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27590 XM_005217447.3
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27590 XM_015475329.1
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27590 XM_005217446.3
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27590 XM_015475330.1
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa224140 XM_024977002.1
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa224141 XM_015475328.2
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa224142 XM_005217446.4
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa224142 XM_005217447.4
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa224142 XM_015475329.2
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa224143 XM_024977003.1
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa93094 XM_015475328.1
Latest version!
Bos taurus toll like receptor 2 (TLR2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa01524
    Clone ID Related Accession (Same CDS sequence) NM_174197.2
    Accession Version NM_174197.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-03
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF368419.1. On Jun 3, 2003 this sequence version replaced NM_174197.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF368419.1, AY634629.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145442, SAMN04335740 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGATA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TCACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCATGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776622.1
    CDS197..2551
    Translation

    Target ORF information:

    RefSeq Version NM_174197.2
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174197.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGATA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TCACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCATGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27590
    Clone ID Related Accession (Same CDS sequence) XM_015475330.1 , XM_005217446.3 , XM_015475329.1 , XM_005217447.3
    Accession Version XM_015475330.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104445.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TTACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCACGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330816.1
    CDS38..2392
    Translation

    Target ORF information:

    RefSeq Version XM_015475330.1
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475330.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TTACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCACGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27590
    Clone ID Related Accession (Same CDS sequence) XM_015475330.1 , XM_005217446.3 , XM_015475329.1 , XM_005217447.3
    Accession Version XM_005217446.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104445.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TTACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCACGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217503.1
    CDS228..2582
    Translation

    Target ORF information:

    RefSeq Version XM_005217446.3
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217446.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TTACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCACGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27590
    Clone ID Related Accession (Same CDS sequence) XM_015475330.1 , XM_005217446.3 , XM_015475329.1 , XM_005217447.3
    Accession Version XM_015475329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104445.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TTACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCACGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330815.1
    CDS229..2583
    Translation

    Target ORF information:

    RefSeq Version XM_015475329.1
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TTACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCACGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27590
    Clone ID Related Accession (Same CDS sequence) XM_015475330.1 , XM_005217446.3 , XM_015475329.1 , XM_005217447.3
    Accession Version XM_005217447.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104445.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217447.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TTACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCACGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217504.1
    CDS205..2559
    Translation

    Target ORF information:

    RefSeq Version XM_005217447.3
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217447.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TTACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCACGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224140
    Clone ID Related Accession (Same CDS sequence) XM_024977002.1
    Accession Version XM_024977002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTGTCTA AGCCCCAGGC GGCCAGCACA GCCCAGGCCA CCTTGTGGGG AAGAGGTTCC 
    GCAGAGCCAG CAGACACTGC CTTTGAGCCC CGCCATCCCA AGAGCGCCCC AAAGTCACTC
    ACCCAAGGCA GCAGCGCCTG CGCCAACGGG CTCCCACATC CTGGGGGAGC GAGATGCAGC
    TCATACCCCC GTGGAGATCT GACTCCTCCA ACACAGTCAA ATCACTGGAC AATGCCACGT
    GCTTTGTGGA CAGCATGGGT CTGGGCTGTC ATCATCCTGT CCACGGAAGG AGCCTCTGAC
    CAGGCTTCTT CTCTGTCTTG TGACCCAACT GGTGTCTGCG ATGGCCATTC CAGATCTTTA
    AACTCCATCC CCTCTGGTCT CACGGCAGGT GTGAAAAGCC TTGACCTGTC CAACAATGAG
    ATCACCTATG TCGGCAACAG AGACCTGCAG AGGTGTGTGA ACCTGAAGAC TCTGAGGCTG
    GGGGCCAATG AAATTCACAC AGTGGAGGAA GATTCTTTTT TTCACCTGAG GAATCTTGAA
    TATTTGGACT TATCCTATAA TCGCTTATCT AACTTATCAT CCTCCTGGTT CAGGTCCCTT
    TATGTCTTGA AATTCTTAAA CTTACTGGGA AATTTATACA AAACACTTGG GGAAACATCT
    CTTTTTTCTC ATCTCCCAAA TCTGCGGACC CTAAAAGTAG GAAATAGTAA CAGCTTCACT
    GAGATTCATG AAAAGGATTT CACTGGACTG ACTTTTCTTG AGGAGCTTGA GATCAGTGCT
    CAAAATCTGC AGATATATGT GCCAAAGAGT TTAAAGTCAA TCCAGAACAT TAGCCATCTG
    ATTCTACATC TGAAGCAGCC TATTTTACTC GTGGACATTC TTGTAGATAT TGTAAGTTCC
    TTAGATTGTT TTGAACTGAG AGATACTAAT TTGCACACTT TCCATTTTTC AGAAGCATCC
    ATCAGTGAAA TGAGTACATC GGTTAAAAAG CTTATATTTA GAAATGTGCA ATTCACCGAT
    GAAAGTTTTG TTGAAGTTGT CAAACTGTTT AACTATGTTT CTGGGATCTT AGAAGTAGAG
    TTTGATGACT GTACCCATGA TGGAATTGGC GATTTTAGAG CACTGAGTTT GGACAGAATT
    AGACACCTAG GTAATGTGGA GACGTTAACA ATACGGAAGT TGCATATTCC ACAGTTTTTC
    TTATTTCATG ATCTGAGTAG TATATATCCA CTCACAGGCA GAGTTAAAAG AGTCACAATA
    GAAAACAGTA AGGTTTTTCT GGTTCCTTGT TTACTTTCAC AACATTTAAA ATCATTAGAA
    TATTTGGATC TCAGTGAAAA CTTAATGTCT GAAGAAACCT TGAAAAACTC AGCCTGTAAG
    GATGCCTGGC CCTTCCTTCA AACCTTGGTT TTAAGGCAGA ATCGTTTGAA ATCACTAGAA
    AAAACGGGAG AACTTTTGCT TACTCTGGAA AATCTGAATA ACCTTGATAT CAGTAAGAAT
    AATTTTCTTT CAATGCCTGA AACTTGTCAG TGGCCAGGAA AAATGAAACA GCTGAACTTA
    TCCAGCACAA GGATACACAG TTTAACCCAG TGCCTTCCCC AGACCCTGGA AATTTTAGAT
    GTTAGCAATA ACAATCTCGA TTCATTTTCT TTGATTTTGC CACAACTCAA AGAACTTTAT
    ATTTCCAGAA ATAAGTTGAA GACTCTACCA GATGCCTCCT TCTTACCCGT GTTATCAGTT
    ATGAGAATTA GCAGAAATAT AATAAATACT TTCTCAAAGG AACAACTTGA TTCTTTTCAG
    CAACTGAAGA CGTTGGAGGC CGGTGGCAAC AACTTCATTT GCTCCTGTGA CTTCCTGTCC
    TTCACACAGG GACAGCAGGC ACTGGGCCGT GTCCTGGTCG ACTGGCCCGA TGACTACCGC
    TGTGACTCTC CCTCCCATGT GCGGGGCCAG CGGGTGCAGG ACGCCCGGCT CTCCCTTTCT
    GAATGCCACA GGGCGGCCGT GGTGTCCGCA GCGTGCTGTG CCCTCTTCCT GTTGCTCCTG
    CTCACGGGGG TGCTGTGTCA CCGTTTCCAC GGACTGTGGT ACATGAAGAT GATGTGGGCG
    TGGCTGCAGG CCAAGAGGAA GCCCAGGAAG GCTCCCCGCA GGGACATCTG CTACGACGCC
    TTCGTGTCCT ACAGCGAGCG GGATTCCTAC TGGGTGGAGA ACCTCATGGT CCAGGAGCTG
    GAGCACTTCA ACCCTCCCTT TAAGCTGTGT CTTCATAAGC GAGACTTCAT TCCTGGCAAG
    TGGATTATCG ACAACATCAT TGACTCCATT GAAAAGAGCC ACAAAACCAT CTTTGTGCTT
    TCGGAGAACT TTGTGAAGAG CGAGTGGTGC AAGTATGAGC TGGACTTCTC CCATTTCCGT
    CTCTTTGATG AGAACAATGA TGCTGCCATT CTGATTCTGC TGGAGCCCAT TGACAAGAAG
    GCCATTCCCC AGCGCTTCTG TAAGCTGCGG AAGATCATGA ACACCAAGAC CTACCTGGAG
    TGGCCCGTGG ATGAGACTCA GCAGGAAGGG TTTTGGTTAA ATTTGAGAGC TGCAATAAGG
    TCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832770.1
    CDS104..2689
    Translation

    Target ORF information:

    RefSeq Version XM_024977002.1
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGTGTCTA AGCCCCAGGC GGCCAGCACA GCCCAGGCCA CCTTGTGGGG AAGAGGTTCC 
    GCAGAGCCAG CAGACACTGC CTTTGAGCCC CGCCATCCCA AGAGCGCCCC AAAGTCACTC
    ACCCAAGGCA GCAGCGCCTG CGCCAACGGG CTCCCACATC CTGGGGGAGC GAGATGCAGC
    TCATACCCCC GTGGAGATCT GACTCCTCCA ACACAGTCAA ATCACTGGAC AATGCCACGT
    GCTTTGTGGA CAGCATGGGT CTGGGCTGTC ATCATCCTGT CCACGGAAGG AGCCTCTGAC
    CAGGCTTCTT CTCTGTCTTG TGACCCAACT GGTGTCTGCG ATGGCCATTC CAGATCTTTA
    AACTCCATCC CCTCTGGTCT CACGGCAGGT GTGAAAAGCC TTGACCTGTC CAACAATGAG
    ATCACCTATG TCGGCAACAG AGACCTGCAG AGGTGTGTGA ACCTGAAGAC TCTGAGGCTG
    GGGGCCAATG AAATTCACAC AGTGGAGGAA GATTCTTTTT TTCACCTGAG GAATCTTGAA
    TATTTGGACT TATCCTATAA TCGCTTATCT AACTTATCAT CCTCCTGGTT CAGGTCCCTT
    TATGTCTTGA AATTCTTAAA CTTACTGGGA AATTTATACA AAACACTTGG GGAAACATCT
    CTTTTTTCTC ATCTCCCAAA TCTGCGGACC CTAAAAGTAG GAAATAGTAA CAGCTTCACT
    GAGATTCATG AAAAGGATTT CACTGGACTG ACTTTTCTTG AGGAGCTTGA GATCAGTGCT
    CAAAATCTGC AGATATATGT GCCAAAGAGT TTAAAGTCAA TCCAGAACAT TAGCCATCTG
    ATTCTACATC TGAAGCAGCC TATTTTACTC GTGGACATTC TTGTAGATAT TGTAAGTTCC
    TTAGATTGTT TTGAACTGAG AGATACTAAT TTGCACACTT TCCATTTTTC AGAAGCATCC
    ATCAGTGAAA TGAGTACATC GGTTAAAAAG CTTATATTTA GAAATGTGCA ATTCACCGAT
    GAAAGTTTTG TTGAAGTTGT CAAACTGTTT AACTATGTTT CTGGGATCTT AGAAGTAGAG
    TTTGATGACT GTACCCATGA TGGAATTGGC GATTTTAGAG CACTGAGTTT GGACAGAATT
    AGACACCTAG GTAATGTGGA GACGTTAACA ATACGGAAGT TGCATATTCC ACAGTTTTTC
    TTATTTCATG ATCTGAGTAG TATATATCCA CTCACAGGCA GAGTTAAAAG AGTCACAATA
    GAAAACAGTA AGGTTTTTCT GGTTCCTTGT TTACTTTCAC AACATTTAAA ATCATTAGAA
    TATTTGGATC TCAGTGAAAA CTTAATGTCT GAAGAAACCT TGAAAAACTC AGCCTGTAAG
    GATGCCTGGC CCTTCCTTCA AACCTTGGTT TTAAGGCAGA ATCGTTTGAA ATCACTAGAA
    AAAACGGGAG AACTTTTGCT TACTCTGGAA AATCTGAATA ACCTTGATAT CAGTAAGAAT
    AATTTTCTTT CAATGCCTGA AACTTGTCAG TGGCCAGGAA AAATGAAACA GCTGAACTTA
    TCCAGCACAA GGATACACAG TTTAACCCAG TGCCTTCCCC AGACCCTGGA AATTTTAGAT
    GTTAGCAATA ACAATCTCGA TTCATTTTCT TTGATTTTGC CACAACTCAA AGAACTTTAT
    ATTTCCAGAA ATAAGTTGAA GACTCTACCA GATGCCTCCT TCTTACCCGT GTTATCAGTT
    ATGAGAATTA GCAGAAATAT AATAAATACT TTCTCAAAGG AACAACTTGA TTCTTTTCAG
    CAACTGAAGA CGTTGGAGGC CGGTGGCAAC AACTTCATTT GCTCCTGTGA CTTCCTGTCC
    TTCACACAGG GACAGCAGGC ACTGGGCCGT GTCCTGGTCG ACTGGCCCGA TGACTACCGC
    TGTGACTCTC CCTCCCATGT GCGGGGCCAG CGGGTGCAGG ACGCCCGGCT CTCCCTTTCT
    GAATGCCACA GGGCGGCCGT GGTGTCCGCA GCGTGCTGTG CCCTCTTCCT GTTGCTCCTG
    CTCACGGGGG TGCTGTGTCA CCGTTTCCAC GGACTGTGGT ACATGAAGAT GATGTGGGCG
    TGGCTGCAGG CCAAGAGGAA GCCCAGGAAG GCTCCCCGCA GGGACATCTG CTACGACGCC
    TTCGTGTCCT ACAGCGAGCG GGATTCCTAC TGGGTGGAGA ACCTCATGGT CCAGGAGCTG
    GAGCACTTCA ACCCTCCCTT TAAGCTGTGT CTTCATAAGC GAGACTTCAT TCCTGGCAAG
    TGGATTATCG ACAACATCAT TGACTCCATT GAAAAGAGCC ACAAAACCAT CTTTGTGCTT
    TCGGAGAACT TTGTGAAGAG CGAGTGGTGC AAGTATGAGC TGGACTTCTC CCATTTCCGT
    CTCTTTGATG AGAACAATGA TGCTGCCATT CTGATTCTGC TGGAGCCCAT TGACAAGAAG
    GCCATTCCCC AGCGCTTCTG TAAGCTGCGG AAGATCATGA ACACCAAGAC CTACCTGGAG
    TGGCCCGTGG ATGAGACTCA GCAGGAAGGG TTTTGGTTAA ATTTGAGAGC TGCAATAAGG
    TCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224141
    Clone ID Related Accession (Same CDS sequence) XM_015475328.2
    Accession Version XM_015475328.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCAGGAGG CCAAGTCAAA TCACTGGACA ATGCCACGTG CTTTGTGGAC AGCATGGGTC 
    TGGGCTGTCA TCATCCTGTC CACGGAAGGA GCCTCTGACC AGGCTTCTTC TCTGTCTTGT
    GACCCAACTG GTGTCTGCGA TGGCCATTCC AGATCTTTAA ACTCCATCCC CTCTGGTCTC
    ACGGCAGGTG TGAAAAGCCT TGACCTGTCC AACAATGAGA TCACCTATGT CGGCAACAGA
    GACCTGCAGA GGTGTGTGAA CCTGAAGACT CTGAGGCTGG GGGCCAATGA AATTCACACA
    GTGGAGGAAG ATTCTTTTTT TCACCTGAGG AATCTTGAAT ATTTGGACTT ATCCTATAAT
    CGCTTATCTA ACTTATCATC CTCCTGGTTC AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC
    TTACTGGGAA ATTTATACAA AACACTTGGG GAAACATCTC TTTTTTCTCA TCTCCCAAAT
    CTGCGGACCC TAAAAGTAGG AAATAGTAAC AGCTTCACTG AGATTCATGA AAAGGATTTC
    ACTGGACTGA CTTTTCTTGA GGAGCTTGAG ATCAGTGCTC AAAATCTGCA GATATATGTG
    CCAAAGAGTT TAAAGTCAAT CCAGAACATT AGCCATCTGA TTCTACATCT GAAGCAGCCT
    ATTTTACTCG TGGACATTCT TGTAGATATT GTAAGTTCCT TAGATTGTTT TGAACTGAGA
    GATACTAATT TGCACACTTT CCATTTTTCA GAAGCATCCA TCAGTGAAAT GAGTACATCG
    GTTAAAAAGC TTATATTTAG AAATGTGCAA TTCACCGATG AAAGTTTTGT TGAAGTTGTC
    AAACTGTTTA ACTATGTTTC TGGGATCTTA GAAGTAGAGT TTGATGACTG TACCCATGAT
    GGAATTGGCG ATTTTAGAGC ACTGAGTTTG GACAGAATTA GACACCTAGG TAATGTGGAG
    ACGTTAACAA TACGGAAGTT GCATATTCCA CAGTTTTTCT TATTTCATGA TCTGAGTAGT
    ATATATCCAC TCACAGGCAG AGTTAAAAGA GTCACAATAG AAAACAGTAA GGTTTTTCTG
    GTTCCTTGTT TACTTTCACA ACATTTAAAA TCATTAGAAT ATTTGGATCT CAGTGAAAAC
    TTAATGTCTG AAGAAACCTT GAAAAACTCA GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA
    ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA TCACTAGAAA AAACGGGAGA ACTTTTGCTT
    ACTCTGGAAA ATCTGAATAA CCTTGATATC AGTAAGAATA ATTTTCTTTC AATGCCTGAA
    ACTTGTCAGT GGCCAGGAAA AATGAAACAG CTGAACTTAT CCAGCACAAG GATACACAGT
    TTAACCCAGT GCCTTCCCCA GACCCTGGAA ATTTTAGATG TTAGCAATAA CAATCTCGAT
    TCATTTTCTT TGATTTTGCC ACAACTCAAA GAACTTTATA TTTCCAGAAA TAAGTTGAAG
    ACTCTACCAG ATGCCTCCTT CTTACCCGTG TTATCAGTTA TGAGAATTAG CAGAAATATA
    ATAAATACTT TCTCAAAGGA ACAACTTGAT TCTTTTCAGC AACTGAAGAC GTTGGAGGCC
    GGTGGCAACA ACTTCATTTG CTCCTGTGAC TTCCTGTCCT TCACACAGGG ACAGCAGGCA
    CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT GACTACCGCT GTGACTCTCC CTCCCATGTG
    CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC TCCCTTTCTG AATGCCACAG GGCGGCCGTG
    GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG TTGCTCCTGC TCACGGGGGT GCTGTGTCAC
    CGTTTCCACG GACTGTGGTA CATGAAGATG ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG
    CCCAGGAAGG CTCCCCGCAG GGACATCTGC TACGACGCCT TCGTGTCCTA CAGCGAGCGG
    GATTCCTACT GGGTGGAGAA CCTCATGGTC CAGGAGCTGG AGCACTTCAA CCCTCCCTTT
    AAGCTGTGTC TTCATAAGCG AGACTTCATT CCTGGCAAGT GGATTATCGA CAACATCATT
    GACTCCATTG AAAAGAGCCA CAAAACCATC TTTGTGCTTT CGGAGAACTT TGTGAAGAGC
    GAGTGGTGCA AGTATGAGCT GGACTTCTCC CATTTCCGTC TCTTTGATGA GAACAATGAT
    GCTGCCATTC TGATTCTGCT GGAGCCCATT GACAAGAAGG CCATTCCCCA GCGCTTCTGT
    AAGCTGCGGA AGATCATGAA CACCAAGACC TACCTGGAGT GGCCCGTGGA TGAGACTCAG
    CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330814.1
    CDS8..2392
    Translation

    Target ORF information:

    RefSeq Version XM_015475328.2
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCAGGAGG CCAAGTCAAA TCACTGGACA ATGCCACGTG CTTTGTGGAC AGCATGGGTC 
    TGGGCTGTCA TCATCCTGTC CACGGAAGGA GCCTCTGACC AGGCTTCTTC TCTGTCTTGT
    GACCCAACTG GTGTCTGCGA TGGCCATTCC AGATCTTTAA ACTCCATCCC CTCTGGTCTC
    ACGGCAGGTG TGAAAAGCCT TGACCTGTCC AACAATGAGA TCACCTATGT CGGCAACAGA
    GACCTGCAGA GGTGTGTGAA CCTGAAGACT CTGAGGCTGG GGGCCAATGA AATTCACACA
    GTGGAGGAAG ATTCTTTTTT TCACCTGAGG AATCTTGAAT ATTTGGACTT ATCCTATAAT
    CGCTTATCTA ACTTATCATC CTCCTGGTTC AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC
    TTACTGGGAA ATTTATACAA AACACTTGGG GAAACATCTC TTTTTTCTCA TCTCCCAAAT
    CTGCGGACCC TAAAAGTAGG AAATAGTAAC AGCTTCACTG AGATTCATGA AAAGGATTTC
    ACTGGACTGA CTTTTCTTGA GGAGCTTGAG ATCAGTGCTC AAAATCTGCA GATATATGTG
    CCAAAGAGTT TAAAGTCAAT CCAGAACATT AGCCATCTGA TTCTACATCT GAAGCAGCCT
    ATTTTACTCG TGGACATTCT TGTAGATATT GTAAGTTCCT TAGATTGTTT TGAACTGAGA
    GATACTAATT TGCACACTTT CCATTTTTCA GAAGCATCCA TCAGTGAAAT GAGTACATCG
    GTTAAAAAGC TTATATTTAG AAATGTGCAA TTCACCGATG AAAGTTTTGT TGAAGTTGTC
    AAACTGTTTA ACTATGTTTC TGGGATCTTA GAAGTAGAGT TTGATGACTG TACCCATGAT
    GGAATTGGCG ATTTTAGAGC ACTGAGTTTG GACAGAATTA GACACCTAGG TAATGTGGAG
    ACGTTAACAA TACGGAAGTT GCATATTCCA CAGTTTTTCT TATTTCATGA TCTGAGTAGT
    ATATATCCAC TCACAGGCAG AGTTAAAAGA GTCACAATAG AAAACAGTAA GGTTTTTCTG
    GTTCCTTGTT TACTTTCACA ACATTTAAAA TCATTAGAAT ATTTGGATCT CAGTGAAAAC
    TTAATGTCTG AAGAAACCTT GAAAAACTCA GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA
    ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA TCACTAGAAA AAACGGGAGA ACTTTTGCTT
    ACTCTGGAAA ATCTGAATAA CCTTGATATC AGTAAGAATA ATTTTCTTTC AATGCCTGAA
    ACTTGTCAGT GGCCAGGAAA AATGAAACAG CTGAACTTAT CCAGCACAAG GATACACAGT
    TTAACCCAGT GCCTTCCCCA GACCCTGGAA ATTTTAGATG TTAGCAATAA CAATCTCGAT
    TCATTTTCTT TGATTTTGCC ACAACTCAAA GAACTTTATA TTTCCAGAAA TAAGTTGAAG
    ACTCTACCAG ATGCCTCCTT CTTACCCGTG TTATCAGTTA TGAGAATTAG CAGAAATATA
    ATAAATACTT TCTCAAAGGA ACAACTTGAT TCTTTTCAGC AACTGAAGAC GTTGGAGGCC
    GGTGGCAACA ACTTCATTTG CTCCTGTGAC TTCCTGTCCT TCACACAGGG ACAGCAGGCA
    CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT GACTACCGCT GTGACTCTCC CTCCCATGTG
    CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC TCCCTTTCTG AATGCCACAG GGCGGCCGTG
    GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG TTGCTCCTGC TCACGGGGGT GCTGTGTCAC
    CGTTTCCACG GACTGTGGTA CATGAAGATG ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG
    CCCAGGAAGG CTCCCCGCAG GGACATCTGC TACGACGCCT TCGTGTCCTA CAGCGAGCGG
    GATTCCTACT GGGTGGAGAA CCTCATGGTC CAGGAGCTGG AGCACTTCAA CCCTCCCTTT
    AAGCTGTGTC TTCATAAGCG AGACTTCATT CCTGGCAAGT GGATTATCGA CAACATCATT
    GACTCCATTG AAAAGAGCCA CAAAACCATC TTTGTGCTTT CGGAGAACTT TGTGAAGAGC
    GAGTGGTGCA AGTATGAGCT GGACTTCTCC CATTTCCGTC TCTTTGATGA GAACAATGAT
    GCTGCCATTC TGATTCTGCT GGAGCCCATT GACAAGAAGG CCATTCCCCA GCGCTTCTGT
    AAGCTGCGGA AGATCATGAA CACCAAGACC TACCTGGAGT GGCCCGTGGA TGAGACTCAG
    CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224142
    Clone ID Related Accession (Same CDS sequence) XM_005217446.4 , XM_015475329.2 , XM_005217447.4
    Accession Version XM_005217446.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217446.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TCACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCATGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217503.1
    CDS240..2594
    Translation

    Target ORF information:

    RefSeq Version XM_005217446.4
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217446.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TCACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCATGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224142
    Clone ID Related Accession (Same CDS sequence) XM_005217446.4 , XM_015475329.2 , XM_005217447.4
    Accession Version XM_015475329.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TCACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCATGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330815.1
    CDS227..2581
    Translation

    Target ORF information:

    RefSeq Version XM_015475329.2
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475329.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TCACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCATGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224142
    Clone ID Related Accession (Same CDS sequence) XM_005217446.4 , XM_015475329.2 , XM_005217447.4
    Accession Version XM_005217447.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217447.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TCACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCATGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217504.1
    CDS875..3229
    Translation

    Target ORF information:

    RefSeq Version XM_005217447.4
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217447.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCCACGTG CTTTGTGGAC AGCATGGGTC TGGGCTGTCA TCATCCTGTC CACGGAAGGA 
    GCCTCTGACC AGGCTTCTTC TCTGTCTTGT GACCCAACTG GTGTCTGCGA TGGCCATTCC
    AGATCTTTAA ACTCCATCCC CTCTGGTCTC ACGGCAGGTG TGAAAAGCCT TGACCTGTCC
    AACAATGAGA TCACCTATGT CGGCAACAGA GACCTGCAGA GGTGTGTGAA CCTGAAGACT
    CTGAGGCTGG GGGCCAATGA AATTCACACA GTGGAGGAAG ATTCTTTTTT TCACCTGAGG
    AATCTTGAAT ATTTGGACTT ATCCTATAAT CGCTTATCTA ACTTATCATC CTCCTGGTTC
    AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC TTACTGGGAA ATTTATACAA AACACTTGGG
    GAAACATCTC TTTTTTCTCA TCTCCCAAAT CTGCGGACCC TAAAAGTAGG AAATAGTAAC
    AGCTTCACTG AGATTCATGA AAAGGATTTC ACTGGACTGA CTTTTCTTGA GGAGCTTGAG
    ATCAGTGCTC AAAATCTGCA GATATATGTG CCAAAGAGTT TAAAGTCAAT CCAGAACATT
    AGCCATCTGA TTCTACATCT GAAGCAGCCT ATTTTACTCG TGGACATTCT TGTAGATATT
    GTAAGTTCCT TAGATTGTTT TGAACTGAGA GATACTAATT TGCACACTTT CCATTTTTCA
    GAAGCATCCA TCAGTGAAAT GAGTACATCG GTTAAAAAGC TTATATTTAG AAATGTGCAA
    TTCACCGATG AAAGTTTTGT TGAAGTTGTC AAACTGTTTA ACTATGTTTC TGGGATCTTA
    GAAGTAGAGT TTGATGACTG TACCCATGAT GGAATTGGCG ATTTTAGAGC ACTGAGTTTG
    GACAGAATTA GACACCTAGG TAATGTGGAG ACGTTAACAA TACGGAAGTT GCATATTCCA
    CAGTTTTTCT TATTTCATGA TCTGAGTAGT ATATATCCAC TCACAGGCAG AGTTAAAAGA
    GTCACAATAG AAAACAGTAA GGTTTTTCTG GTTCCTTGTT TACTTTCACA ACATTTAAAA
    TCATTAGAAT ATTTGGATCT CAGTGAAAAC TTAATGTCTG AAGAAACCTT GAAAAACTCA
    GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA
    TCACTAGAAA AAACGGGAGA ACTTTTGCTT ACTCTGGAAA ATCTGAATAA CCTTGATATC
    AGTAAGAATA ATTTTCTTTC AATGCCTGAA ACTTGTCAGT GGCCAGGAAA AATGAAACAG
    CTGAACTTAT CCAGCACAAG GATACACAGT TTAACCCAGT GCCTTCCCCA GACCCTGGAA
    ATTTTAGATG TTAGCAATAA CAATCTCGAT TCATTTTCTT TGATTTTGCC ACAACTCAAA
    GAACTTTATA TTTCCAGAAA TAAGTTGAAG ACTCTACCAG ATGCCTCCTT CTTACCCGTG
    TTATCAGTTA TGAGAATTAG CAGAAATATA ATAAATACTT TCTCAAAGGA ACAACTTGAT
    TCTTTTCAGC AACTGAAGAC GTTGGAGGCC GGTGGCAACA ACTTCATTTG CTCCTGTGAC
    TTCCTGTCCT TCACACAGGG ACAGCAGGCA CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT
    GACTACCGCT GTGACTCTCC CTCCCATGTG CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC
    TCCCTTTCTG AATGCCACAG GGCGGCCGTG GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG
    TTGCTCCTGC TCACGGGGGT GCTGTGTCAC CGTTTCCACG GACTGTGGTA CATGAAGATG
    ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG CCCAGGAAGG CTCCCCGCAG GGACATCTGC
    TACGACGCCT TCGTGTCCTA CAGCGAGCGG GATTCCTACT GGGTGGAGAA CCTCATGGTC
    CAGGAGCTGG AGCACTTCAA CCCTCCCTTT AAGCTGTGTC TTCATAAGCG AGACTTCATT
    CCTGGCAAGT GGATTATCGA CAACATCATT GACTCCATTG AAAAGAGCCA CAAAACCATC
    TTTGTGCTTT CGGAGAACTT TGTGAAGAGC GAGTGGTGCA AGTATGAGCT GGACTTCTCC
    CATTTCCGTC TCTTTGATGA GAACAATGAT GCTGCCATTC TGATTCTGCT GGAGCCCATT
    GACAAGAAGG CCATTCCCCA GCGCTTCTGT AAGCTGCGGA AGATCATGAA CACCAAGACC
    TACCTGGAGT GGCCCGTGGA TGAGACTCAG CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT
    GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224143
    Clone ID Related Accession (Same CDS sequence) XM_024977003.1
    Accession Version XM_024977003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGTGTCTA AGCCCCAGGC GGCCAGCACA GCCCAGGCCA CCTTGTGGGG AAGAGGTTCC 
    GCAGAGCCAG CAGACACTGC CTTTGAGCCC CGCCATCCCA AGAGCGCCCC AAAGTCACTC
    ACCCAAGGCA GCAGCGCCTG CGCCAACGGG CTCCCACATC CTGGGGGAGC GAGATGCAGC
    TCATACCCCC GTGGAGATCT GACTCCTCCA ACACAGTCAA ATCACTGGAC AATGCCACGT
    GCTTTGTGGA CAGCATGGGT CTGGGCTGTC ATCATCCTGT CCACGGAAGG AGCCTCTGAC
    CAGGCTTCTT CTCTGTCTTG TGACCCAACT GGTGTCTGCG ATGGCCATTC CAGATCTTTA
    AACTCCATCC CCTCTGGTCT CACGGCAGGT GTGAAAAGCC TTGACCTGTC CAACAATGAG
    ATCACCTATG TCGGCAACAG AGACCTGCAG AGGTGTGTGA ACCTGAAGAC TCTGAGGCTG
    GGGGCCAATG AAATTCACAC AGTGGAGGAA GATTCTTTTT TTCACCTGAG GAATCTTGAA
    TATTTGGACT TATCCTATAA TCGCTTATCT AACTTATCAT CCTCCTGGTT CAGGTCCCTT
    TATGTCTTGA AATTCTTAAA CTTACTGGGA AATTTATACA AAACACTTGG GGAAACATCT
    CTTTTTTCTC ATCTCCCAAA TCTGCGGACC CTAAAAGTAG GAAATAGTAA CAGCTTCACT
    GAGATTCATG AAAAGGATTT CACTGGACTG ACTTTTCTTG AGGAGCTTGA GATCAGTGCT
    CAAAATCTGC AGATATATGT GCCAAAGAGT TTAAAGTCAA TCCAGAACAT TAGCCATCTG
    ATTCTACATC TGAAGCAGCC TATTTTACTC GTGGACATTC TTGTAGATAT TCAACTGAAG
    ACGTTGGAGG CCGGTGGCAA CAACTTCATT TGCTCCTGTG ACTTCCTGTC CTTCACACAG
    GGACAGCAGG CACTGGGCCG TGTCCTGGTC GACTGGCCCG ATGACTACCG CTGTGACTCT
    CCCTCCCATG TGCGGGGCCA GCGGGTGCAG GACGCCCGGC TCTCCCTTTC TGAATGCCAC
    AGGGCGGCCG TGGTGTCCGC AGCGTGCTGT GCCCTCTTCC TGTTGCTCCT GCTCACGGGG
    GTGCTGTGTC ACCGTTTCCA CGGACTGTGG TACATGAAGA TGATGTGGGC GTGGCTGCAG
    GCCAAGAGGA AGCCCAGGAA GGCTCCCCGC AGGGACATCT GCTACGACGC CTTCGTGTCC
    TACAGCGAGC GGGATTCCTA CTGGGTGGAG AACCTCATGG TCCAGGAGCT GGAGCACTTC
    AACCCTCCCT TTAAGCTGTG TCTTCATAAG CGAGACTTCA TTCCTGGCAA GTGGATTATC
    GACAACATCA TTGACTCCAT TGAAAAGAGC CACAAAACCA TCTTTGTGCT TTCGGAGAAC
    TTTGTGAAGA GCGAGTGGTG CAAGTATGAG CTGGACTTCT CCCATTTCCG TCTCTTTGAT
    GAGAACAATG ATGCTGCCAT TCTGATTCTG CTGGAGCCCA TTGACAAGAA GGCCATTCCC
    CAGCGCTTCT GTAAGCTGCG GAAGATCATG AACACCAAGA CCTACCTGGA GTGGCCCGTG
    GATGAGACTC AGCAGGAAGG GTTTTGGTTA AATTTGAGAG CTGCAATAAG GTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832771.1
    CDS104..1780
    Translation

    Target ORF information:

    RefSeq Version XM_024977003.1
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGTGTCTA AGCCCCAGGC GGCCAGCACA GCCCAGGCCA CCTTGTGGGG AAGAGGTTCC 
    GCAGAGCCAG CAGACACTGC CTTTGAGCCC CGCCATCCCA AGAGCGCCCC AAAGTCACTC
    ACCCAAGGCA GCAGCGCCTG CGCCAACGGG CTCCCACATC CTGGGGGAGC GAGATGCAGC
    TCATACCCCC GTGGAGATCT GACTCCTCCA ACACAGTCAA ATCACTGGAC AATGCCACGT
    GCTTTGTGGA CAGCATGGGT CTGGGCTGTC ATCATCCTGT CCACGGAAGG AGCCTCTGAC
    CAGGCTTCTT CTCTGTCTTG TGACCCAACT GGTGTCTGCG ATGGCCATTC CAGATCTTTA
    AACTCCATCC CCTCTGGTCT CACGGCAGGT GTGAAAAGCC TTGACCTGTC CAACAATGAG
    ATCACCTATG TCGGCAACAG AGACCTGCAG AGGTGTGTGA ACCTGAAGAC TCTGAGGCTG
    GGGGCCAATG AAATTCACAC AGTGGAGGAA GATTCTTTTT TTCACCTGAG GAATCTTGAA
    TATTTGGACT TATCCTATAA TCGCTTATCT AACTTATCAT CCTCCTGGTT CAGGTCCCTT
    TATGTCTTGA AATTCTTAAA CTTACTGGGA AATTTATACA AAACACTTGG GGAAACATCT
    CTTTTTTCTC ATCTCCCAAA TCTGCGGACC CTAAAAGTAG GAAATAGTAA CAGCTTCACT
    GAGATTCATG AAAAGGATTT CACTGGACTG ACTTTTCTTG AGGAGCTTGA GATCAGTGCT
    CAAAATCTGC AGATATATGT GCCAAAGAGT TTAAAGTCAA TCCAGAACAT TAGCCATCTG
    ATTCTACATC TGAAGCAGCC TATTTTACTC GTGGACATTC TTGTAGATAT TCAACTGAAG
    ACGTTGGAGG CCGGTGGCAA CAACTTCATT TGCTCCTGTG ACTTCCTGTC CTTCACACAG
    GGACAGCAGG CACTGGGCCG TGTCCTGGTC GACTGGCCCG ATGACTACCG CTGTGACTCT
    CCCTCCCATG TGCGGGGCCA GCGGGTGCAG GACGCCCGGC TCTCCCTTTC TGAATGCCAC
    AGGGCGGCCG TGGTGTCCGC AGCGTGCTGT GCCCTCTTCC TGTTGCTCCT GCTCACGGGG
    GTGCTGTGTC ACCGTTTCCA CGGACTGTGG TACATGAAGA TGATGTGGGC GTGGCTGCAG
    GCCAAGAGGA AGCCCAGGAA GGCTCCCCGC AGGGACATCT GCTACGACGC CTTCGTGTCC
    TACAGCGAGC GGGATTCCTA CTGGGTGGAG AACCTCATGG TCCAGGAGCT GGAGCACTTC
    AACCCTCCCT TTAAGCTGTG TCTTCATAAG CGAGACTTCA TTCCTGGCAA GTGGATTATC
    GACAACATCA TTGACTCCAT TGAAAAGAGC CACAAAACCA TCTTTGTGCT TTCGGAGAAC
    TTTGTGAAGA GCGAGTGGTG CAAGTATGAG CTGGACTTCT CCCATTTCCG TCTCTTTGAT
    GAGAACAATG ATGCTGCCAT TCTGATTCTG CTGGAGCCCA TTGACAAGAA GGCCATTCCC
    CAGCGCTTCT GTAAGCTGCG GAAGATCATG AACACCAAGA CCTACCTGGA GTGGCCCGTG
    GATGAGACTC AGCAGGAAGG GTTTTGGTTA AATTTGAGAG CTGCAATAAG GTCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93094
    Clone ID Related Accession (Same CDS sequence) XM_015475328.1
    Accession Version XM_015475328.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product toll-like receptor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104445.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCAGGAGG CCAAGTCAAA TCACTGGACA ATGCCACGTG CTTTGTGGAC AGCATGGGTC 
    TGGGCTGTCA TCATCCTGTC CACGGAAGGA GCCTCTGACC AGGCTTCTTC TCTGTCTTGT
    GACCCAACTG GTGTCTGCGA TGGCCATTCC AGATCTTTAA ACTCCATCCC CTCTGGTCTC
    ACGGCAGGTG TGAAAAGCCT TGACCTGTCC AACAATGAGA TCACCTATGT CGGCAACAGA
    GACCTGCAGA GGTGTGTGAA CCTGAAGACT CTGAGGCTGG GGGCCAATGA AATTCACACA
    GTGGAGGAAG ATTCTTTTTT TCACCTGAGG AATCTTGAAT ATTTGGACTT ATCCTATAAT
    CGCTTATCTA ACTTATCATC CTCCTGGTTC AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC
    TTACTGGGAA ATTTATACAA AACACTTGGG GAAACATCTC TTTTTTCTCA TCTCCCAAAT
    CTGCGGACCC TAAAAGTAGG AAATAGTAAC AGCTTCACTG AGATTCATGA AAAGGATTTC
    ACTGGACTGA CTTTTCTTGA GGAGCTTGAG ATCAGTGCTC AAAATCTGCA GATATATGTG
    CCAAAGAGTT TAAAGTCAAT CCAGAACATT AGCCATCTGA TTCTACATCT GAAGCAGCCT
    ATTTTACTCG TGGACATTCT TGTAGATATT GTAAGTTCCT TAGATTGTTT TGAACTGAGA
    GATACTAATT TGCACACTTT CCATTTTTCA GAAGCATCCA TCAGTGAAAT GAGTACATCG
    GTTAAAAAGC TTATATTTAG AAATGTGCAA TTCACCGATG AAAGTTTTGT TGAAGTTGTC
    AAACTGTTTA ACTATGTTTC TGGGATCTTA GAAGTAGAGT TTGATGACTG TACCCATGAT
    GGAATTGGCG ATTTTAGAGC ACTGAGTTTG GACAGAATTA GACACCTAGG TAATGTGGAG
    ACGTTAACAA TACGGAAGTT GCATATTCCA CAGTTTTTCT TATTTCATGA TCTGAGTAGT
    ATATATCCAC TCACAGGCAG AGTTAAAAGA GTCACAATAG AAAACAGTAA GGTTTTTCTG
    GTTCCTTGTT TACTTTCACA ACATTTAAAA TCATTAGAAT ATTTGGATCT CAGTGAAAAC
    TTAATGTCTG AAGAAACCTT GAAAAACTCA GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA
    ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA TCACTAGAAA AAACGGGAGA ACTTTTGCTT
    ACTCTGGAAA ATCTGAATAA CCTTGATATC AGTAAGAATA ATTTTCTTTC AATGCCTGAA
    ACTTGTCAGT GGCCAGGAAA AATGAAACAG CTGAACTTAT CCAGCACAAG GATACACAGT
    TTAACCCAGT GCCTTCCCCA GACCCTGGAA ATTTTAGATG TTAGCAATAA CAATCTCGAT
    TCATTTTCTT TGATTTTGCC ACAACTCAAA GAACTTTATA TTTCCAGAAA TAAGTTGAAG
    ACTCTACCAG ATGCCTCCTT CTTACCCGTG TTATCAGTTA TGAGAATTAG CAGAAATATA
    ATAAATACTT TCTCAAAGGA ACAACTTGAT TCTTTTCAGC AACTGAAGAC GTTGGAGGCC
    GGTGGCAACA ACTTCATTTG CTCCTGTGAC TTCCTGTCCT TTACACAGGG ACAGCAGGCA
    CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT GACTACCGCT GTGACTCTCC CTCCCACGTG
    CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC TCCCTTTCTG AATGCCACAG GGCGGCCGTG
    GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG TTGCTCCTGC TCACGGGGGT GCTGTGTCAC
    CGTTTCCACG GACTGTGGTA CATGAAGATG ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG
    CCCAGGAAGG CTCCCCGCAG GGACATCTGC TACGACGCCT TCGTGTCCTA CAGCGAGCGG
    GATTCCTACT GGGTGGAGAA CCTCATGGTC CAGGAGCTGG AGCACTTCAA CCCTCCCTTT
    AAGCTGTGTC TTCATAAGCG AGACTTCATT CCTGGCAAGT GGATTATCGA CAACATCATT
    GACTCCATTG AAAAGAGCCA CAAAACCATC TTTGTGCTTT CGGAGAACTT TGTGAAGAGC
    GAGTGGTGCA AGTATGAGCT GGACTTCTCC CATTTCCGTC TCTTTGATGA GAACAATGAT
    GCTGCCATTC TGATTCTGCT GGAGCCCATT GACAAGAAGG CCATTCCCCA GCGCTTCTGT
    AAGCTGCGGA AGATCATGAA CACCAAGACC TACCTGGAGT GGCCCGTGGA TGAGACTCAG
    CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330814.1
    CDS54..2438
    Translation

    Target ORF information:

    RefSeq Version XM_015475328.1
    Organism Bos taurus(cattle)
    Definition Bos taurus toll like receptor 2 (TLR2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCAGGAGG CCAAGTCAAA TCACTGGACA ATGCCACGTG CTTTGTGGAC AGCATGGGTC 
    TGGGCTGTCA TCATCCTGTC CACGGAAGGA GCCTCTGACC AGGCTTCTTC TCTGTCTTGT
    GACCCAACTG GTGTCTGCGA TGGCCATTCC AGATCTTTAA ACTCCATCCC CTCTGGTCTC
    ACGGCAGGTG TGAAAAGCCT TGACCTGTCC AACAATGAGA TCACCTATGT CGGCAACAGA
    GACCTGCAGA GGTGTGTGAA CCTGAAGACT CTGAGGCTGG GGGCCAATGA AATTCACACA
    GTGGAGGAAG ATTCTTTTTT TCACCTGAGG AATCTTGAAT ATTTGGACTT ATCCTATAAT
    CGCTTATCTA ACTTATCATC CTCCTGGTTC AGGTCCCTTT ATGTCTTGAA ATTCTTAAAC
    TTACTGGGAA ATTTATACAA AACACTTGGG GAAACATCTC TTTTTTCTCA TCTCCCAAAT
    CTGCGGACCC TAAAAGTAGG AAATAGTAAC AGCTTCACTG AGATTCATGA AAAGGATTTC
    ACTGGACTGA CTTTTCTTGA GGAGCTTGAG ATCAGTGCTC AAAATCTGCA GATATATGTG
    CCAAAGAGTT TAAAGTCAAT CCAGAACATT AGCCATCTGA TTCTACATCT GAAGCAGCCT
    ATTTTACTCG TGGACATTCT TGTAGATATT GTAAGTTCCT TAGATTGTTT TGAACTGAGA
    GATACTAATT TGCACACTTT CCATTTTTCA GAAGCATCCA TCAGTGAAAT GAGTACATCG
    GTTAAAAAGC TTATATTTAG AAATGTGCAA TTCACCGATG AAAGTTTTGT TGAAGTTGTC
    AAACTGTTTA ACTATGTTTC TGGGATCTTA GAAGTAGAGT TTGATGACTG TACCCATGAT
    GGAATTGGCG ATTTTAGAGC ACTGAGTTTG GACAGAATTA GACACCTAGG TAATGTGGAG
    ACGTTAACAA TACGGAAGTT GCATATTCCA CAGTTTTTCT TATTTCATGA TCTGAGTAGT
    ATATATCCAC TCACAGGCAG AGTTAAAAGA GTCACAATAG AAAACAGTAA GGTTTTTCTG
    GTTCCTTGTT TACTTTCACA ACATTTAAAA TCATTAGAAT ATTTGGATCT CAGTGAAAAC
    TTAATGTCTG AAGAAACCTT GAAAAACTCA GCCTGTAAGG ATGCCTGGCC CTTCCTTCAA
    ACCTTGGTTT TAAGGCAGAA TCGTTTGAAA TCACTAGAAA AAACGGGAGA ACTTTTGCTT
    ACTCTGGAAA ATCTGAATAA CCTTGATATC AGTAAGAATA ATTTTCTTTC AATGCCTGAA
    ACTTGTCAGT GGCCAGGAAA AATGAAACAG CTGAACTTAT CCAGCACAAG GATACACAGT
    TTAACCCAGT GCCTTCCCCA GACCCTGGAA ATTTTAGATG TTAGCAATAA CAATCTCGAT
    TCATTTTCTT TGATTTTGCC ACAACTCAAA GAACTTTATA TTTCCAGAAA TAAGTTGAAG
    ACTCTACCAG ATGCCTCCTT CTTACCCGTG TTATCAGTTA TGAGAATTAG CAGAAATATA
    ATAAATACTT TCTCAAAGGA ACAACTTGAT TCTTTTCAGC AACTGAAGAC GTTGGAGGCC
    GGTGGCAACA ACTTCATTTG CTCCTGTGAC TTCCTGTCCT TTACACAGGG ACAGCAGGCA
    CTGGGCCGTG TCCTGGTCGA CTGGCCCGAT GACTACCGCT GTGACTCTCC CTCCCACGTG
    CGGGGCCAGC GGGTGCAGGA CGCCCGGCTC TCCCTTTCTG AATGCCACAG GGCGGCCGTG
    GTGTCCGCAG CGTGCTGTGC CCTCTTCCTG TTGCTCCTGC TCACGGGGGT GCTGTGTCAC
    CGTTTCCACG GACTGTGGTA CATGAAGATG ATGTGGGCGT GGCTGCAGGC CAAGAGGAAG
    CCCAGGAAGG CTCCCCGCAG GGACATCTGC TACGACGCCT TCGTGTCCTA CAGCGAGCGG
    GATTCCTACT GGGTGGAGAA CCTCATGGTC CAGGAGCTGG AGCACTTCAA CCCTCCCTTT
    AAGCTGTGTC TTCATAAGCG AGACTTCATT CCTGGCAAGT GGATTATCGA CAACATCATT
    GACTCCATTG AAAAGAGCCA CAAAACCATC TTTGTGCTTT CGGAGAACTT TGTGAAGAGC
    GAGTGGTGCA AGTATGAGCT GGACTTCTCC CATTTCCGTC TCTTTGATGA GAACAATGAT
    GCTGCCATTC TGATTCTGCT GGAGCCCATT GACAAGAAGG CCATTCCCCA GCGCTTCTGT
    AAGCTGCGGA AGATCATGAA CACCAAGACC TACCTGGAGT GGCCCGTGGA TGAGACTCAG
    CAGGAAGGGT TTTGGTTAAA TTTGAGAGCT GCAATAAGGT CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.