TLR2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR2
Entrez Gene ID	448807
Full Name	toll like receptor 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022427657.1	XP_022283365.1	toll-like receptor 2 isoform X2
NM_001005264.3	NP_001005264.2	toll-like receptor 2 precursor
XM_005628829.2	XP_005628886.1	toll-like receptor 2 isoform X1
XM_005628829.3	XP_005628886.1	toll-like receptor 2 isoform X2
XM_022427656.1	XP_022283364.1	toll-like receptor 2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TLR2	NP_001123941.1
Gallus gallus (chicken)	LOC100858824	XP_004940943.1
Gallus gallus (chicken)	TLR2-2	NP_001155122.1
Gallus gallus (chicken)	TLR2-1	NP_989609.1
Xenopus tropicalis (tropical clawed frog)	LOC100485927	XP_002933538.2
Homo sapiens (human)	TLR2	NP_003255.2
Canis lupus familiaris (dog)	TLR2	NP_001005264.2
Bos taurus (cattle)	TLR2	NP_776622.1
Rattus norvegicus (Norway rat)	Tlr2	NP_942064.1
Xenopus tropicalis (tropical clawed frog)	LOC100485771	XP_002933537.1
Mus musculus (house mouse)	Tlr2	NP_036035.3
Danio rerio (zebrafish)	tlr2	NP_997977.1
	TLR2	NP_001123897.1

Gene Ontology
Bibliography

Related articles in PubMed

Histopathological findings and detection of Toll-like receptor 2 in cutaneous lesions of canine leishmaniosis.
Esteve LO, Saz SV, Hosein S, Solano-Gallego L
Veterinary parasitology209(3-4)157-63(2015 Apr)

Compartmentalized gene expression of toll-like receptors 2, 4 and 9 in the brain and peripheral lymphoid organs during canine visceral leishmaniasis.
Melo GD, Silva JE, Grano FG, Homem CG, Machado GF
Parasite immunology36(12)726-31(2014 Dec)

Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis.
Mercier E, Peters IR, Farnir F, Lavou? R, Day M, Clercx C, Peeters D
BMC veterinary research10187(2014 Aug)

Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
Figueiredo MM, Amorim IF, Pinto AJ, Barbosa VS, Pinheiro Lde J, Deoti B, Faria AM, Tafuri WL
BMC immunology1422(2013 May)

The localization of Toll-like receptor 2 (TLR2) in the endometrium and the cervix of dogs at different stages of the oestrous cycle and with pyometra.
Chotimanukul S, Sirivaidyapong S
Reproduction in domestic animals = Zuchthygiene47 Suppl 6351-5(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results obtained in this study indicated an association with TLR2 in the pathogenesis of canine cutaneous leishmaniosis.
Title: Histopathological findings and detection of Toll-like receptor 2 in cutaneous lesions of canine leishmaniosis.

Study investigated the pattern of TLR-2, TLR-4 and TLR-9 gene expression in brain, choroid plexus, spleen and lymph node of dogs naturally infected with Leishmania infantum. In the brain, there was only an upregulation of TLR-2, in the choroid plexus, TLR-2 and TLR-9 were both upregulated and peripheral lymphoid organs showed increased TLR-2 and TLR-4 expression.
Title: Compartmentalized gene expression of toll-like receptors 2, 4 and 9 in the brain and peripheral lymphoid organs during canine visceral leishmaniasis.

These findings do not support a role for non-synonymous SNPs in the TLR 2, 4 and 9 coding regions in the pathogenesis of canine SNA, but do not exclude a role for innate immunity in the pathogenesis of the disease.
Title: Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis.

The aim of this study was to investigate the expression of TLR2 and TLR9 in jejunum and colon in dogs naturally infected with Leishmania infantum.
Title: Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.

This study provides the first report of immunohistochemical localization of TLR2 in the canine reproductive tract.
Title: The localization of Toll-like receptor 2 (TLR2) in the endometrium and the cervix of dogs at different stages of the oestrous cycle and with pyometra.

The following TLR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe00281	NM_001005264.3 Latest version!	Canis lupus familiaris toll like receptor 2 (TLR2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe17077	XM_022427657.1 Latest version!	Canis lupus familiaris toll like receptor 2 (TLR2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe17077	XM_005628829.3 Latest version!	Canis lupus familiaris toll like receptor 2 (TLR2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe293351	XM_022427656.1 Latest version!	Canis lupus familiaris toll like receptor 2 (TLR2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OCe17077	XM_005628829.2	Canis lupus familiaris toll-like receptor 2 (TLR2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR2 ( NM_001005264.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001005264.2 Canis lupus familiaris toll like receptor 2 (TLR2), mRNA.

Price & Availability↑

CloneID	OCe00281
Clone ID Related Accession (Same CDS sequence)	NM_001005264.3
Accession Version	NM_001005264.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Canis lupus familiaris(dog)
Product	toll-like receptor 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU487534.1. On Apr 2, 2013 this sequence version replaced NM_001005264.2. ##Evidence-Data-START## Transcript exon combination :: EU487534.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCACGTG TTTTGTGGAC ATTGTGGGTT TTGGGGGCTG TAACCAACCT CTCCAAGGAA 
GAGGCCCCTG ACCAGTCTTC TTCTCTGTCC TGTGACCCCA CTGGTGTCTG CGATGGCCGC 
TCCAGATCTT TGAACTCCAT GCCCTCAGGG CTCACAGCAG CTGTGAGAAG CCTTGACCTC 
TCCAACAATG AGATCACCTA CATTGGCAAC AGTGACCTTC GGGATTGTGT GAACCTCAAG 
GCTCTGAGGC TGGAGTCTAA TGGAATTAAC ACAATAGAGG AAGAATCTTT TTTTTCCCTG 
TGGAGTCTTG AACATTTGGA CTTATCTTAT AACCTCTTAT CTAACTTATC ATCCTCCTGG 
TTCAGGCCCC TTTCTTCATT GAAGTTCTTA AACCTACTGG GAAATCCTTA CAAATCACTT 
GGGGAAACAC CTCTTTTTTC TCAGCTCACA AATCTAAGAA TTCTGAAAGT AGGAAATATC 
TACAGCTTCA CTGAGATTCA GGATAAGGAT TTTGCTGGGC TAACCTTTCT TGAGGAACTG 
GAGATCGATG CTTCAAATCT CCAGAGGTAT GAGCCAAAGA GTTTGAAATC GATTCAGAGC 
ATCAGCTATC TGGCCCTCCG TATGAAGCAG CCTGTTTTAC TGGTGGAGAT TTTTGTAGAT 
CTTTCCAGTT CCTTGAAACA TTTAGAACTG AGAGATACTC ATTTGGACAC TTTCCACTTT 
TCAGAGGCAT CCATCAATGA AACACATACG TTGGTTAAAA AGTGGACATT TAGAAATGTG 
AAAGTCACCG ATAGAAGTTT TACTGAGGTT GTGAGACTGT TGAATTATGT TTCTGGAGTG 
TTAGAAGTAG AGTTTGAGGA CTGTACCCTT TATGGGCTCG GTGATTTTGA CATACCTGAT 
GTGGACAAAA TTAAAAATAT AGGTCAGATA GAGACACTAA CAGTACGGAG GTTGCATATT 
CCACACTTTT ACTCATTTTA CGATATGAGT AGTATATATT CACTTACAGA AGATGTTAAA 
AGAATCACAG TAGAAAGCAG TAAGGTCTTT CTGGTTCCTT GCTCACTTTC ACAACATTTA 
AAATCCCTAG AATATTTGGA TCTCAGCGAC AATTTAGTGG TTGAAGAATA CTTGAGAAAC 
TCAGCCTGTC AGCATGCTTG GCCCCTCCTG CAAACCTTAA TTTTAAGGCA AAATCGTTTG 
AAATCCTTAG AGAAAACTGG AGAAACTTTG CTTACTTTGA AAAACCTGGT GAACCTTGAT 
ATTAGTAAGA ATAATTATCT TTCTATGCCT GAAACTTGTC AGTGGCCAGA AAAGCTGAAA 
TGTTTGAACT TATCCGACAC AAGAATGCAA AGTATAACCC GTTGCATCCC TCAGACACTG 
GAAATTTTAG ATGTTAGCAA TAATAATCTC GAGTCATTTT CCCTGATTTT GCCACAACTT 
AAAGAACTTT CTATTTCCAG AAATAAGTTG AAGACTCTAC CAGATGCCTC CTTCTTACCC 
ACCTTACAAA TTATGAGAAT CAGCAGAAAC ACAATAAACG CTTTCTCGAA GGAGCAACTG 
GATTCCTTTC ACAGGCTGCA GACCCTGGAG GCCGGTGGCA ACAACTTCCT TTGCTCCTGT 
GAATTCCTGT CTTTCACTCA GGAGCAGCAG GCCCTGGCCG GGCTCCTGGT CGGCTGGCCA 
GAGGACTACC TGTGCCACTC CCCCTCCTAC GTGCGGGGCC AGCGGGTTGG GACCGCCCGG 
CTCCCGGCTT CTGAGTGCCA CCGGACAGCT CTGGTGGCCG CCGTGTGCTG TGTCTTGCTC 
CTGCTGGTCC TGCTCACGGC GGGGGCGTGC CACCATTTCC ACGGGCTGTG GTACCTGAGA 
ATGCTGTGGG CCTGGCTCCA GGCCAAAAGG AAGCCCAGGA AAGCCCCCTC CAGGGACGTC 
TGTTATGACG CCTTTGTGTC TTACAGTGAG CATGATTCCT ACTGGGTGGA GAACCTTCTG 
GTCCAGAAGC TGGAGCACTT CAATCCCCCG TTCAAGTTGT GCCTTCACAA GCGGGACTTT 
ATTCCCGGCA AGTGGATTAT TGACAATATC ATTGACTCCA TCGAGAAGAG CCACAAAACC 
ATCTTTGTGC TTTCTGAAAA CTTCGTGAAA AGCGAGTGGT GCAAGTACGA GCTGGACTTC 
TCCCATTTTC GCCTCTTTGA TGAGAACAAC GATGCTGCCA TCCTCATTCT TCTGGAGCCC 
ATTGAGAAAA AGGCCATCCC CCAGCGATTC TGTAAGCTGC GGAAGATAAT GAACACCAAG 
ACGTACCTGG AGTGGCCCAC CGATGATGCT CAGCAGGAAG GGTTTTGGTT AAATTTGAGA 
ACAGCAATAA AATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005264.2
CDS	6..2363
Translation	MSRVLWTLWVLGAVTNLSKEEAPDQSSSLSCDPTGVCDGRSRSLNSMPSGLTAAVRSLDLSNNEITYIGNSDLRDCVNLKALRLESNGINTIEEESFFSLWSLEHLDLSYNLLSNLSSSWFRPLSSLKFLNLLGNPYKSLGETPLFSQLTNLRILKVGNIYSFTEIQDKDFAGLTFLEELEIDASNLQRYEPKSLKSIQSISYLALRMKQPVLLVEIFVDLSSSLKHLELRDTHLDTFHFSEASINETHTLVKKWTFRNVKVTDRSFTEVVRLLNYVSGVLEVEFEDCTLYGLGDFDIPDVDKIKNIGQIETLTVRRLHIPHFYSFYDMSSIYSLTEDVKRITVESSKVFLVPCSLSQHLKSLEYLDLSDNLVVEEYLRNSACQHAWPLLQTLILRQNRLKSLEKTGETLLTLKNLVNLDISKNNYLSMPETCQWPEKLKCLNLSDTRMQSITRCIPQTLEILDVSNNNLESFSLILPQLKELSISRNKLKTLPDASFLPTLQIMRISRNTINAFSKEQLDSFHRLQTLEAGGNNFLCSCEFLSFTQEQQALAGLLVGWPEDYLCHSPSYVRGQRVGTARLPASECHRTALVAAVCCVLLLLVLLTAGACHHFHGLWYLRMLWAWLQAKRKPRKAPSRDVCYDAFVSYSEHDSYWVENLLVQKLEHFNPPFKLCLHKRDFIPGKWIIDNIIDSIEKSHKTIFVLSENFVKSEWCKYELDFSHFRLFDENNDAAILILLEPIEKKAIPQRFCKLRKIMNTKTYLEWPTDDAQQEGFWLNLRTAIKS

Target ORF information:

RefSeq Version	NM_001005264.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris toll like receptor 2 (TLR2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005264.3

ORF Insert Sequence:

ATGTCACGTG TTTTGTGGAC ATTGTGGGTT TTGGGGGCTG TAACCAACCT CTCCAAGGAA 
GAGGCCCCTG ACCAGTCTTC TTCTCTGTCC TGTGACCCCA CTGGTGTCTG CGATGGCCGC 
TCCAGATCTT TGAACTCCAT GCCCTCAGGG CTCACAGCAG CTGTGAGAAG CCTTGACCTC 
TCCAACAATG AGATCACCTA CATTGGCAAC AGTGACCTTC GGGATTGTGT GAACCTCAAG 
GCTCTGAGGC TGGAGTCTAA TGGAATTAAC ACAATAGAGG AAGAATCTTT TTTTTCCCTG 
TGGAGTCTTG AACATTTGGA CTTATCTTAT AACCTCTTAT CTAACTTATC ATCCTCCTGG 
TTCAGGCCCC TTTCTTCATT GAAGTTCTTA AACCTACTGG GAAATCCTTA CAAATCACTT 
GGGGAAACAC CTCTTTTTTC TCAGCTCACA AATCTAAGAA TTCTGAAAGT AGGAAATATC 
TACAGCTTCA CTGAGATTCA GGATAAGGAT TTTGCTGGGC TAACCTTTCT TGAGGAACTG 
GAGATCGATG CTTCAAATCT CCAGAGGTAT GAGCCAAAGA GTTTGAAATC GATTCAGAGC 
ATCAGCTATC TGGCCCTCCG TATGAAGCAG CCTGTTTTAC TGGTGGAGAT TTTTGTAGAT 
CTTTCCAGTT CCTTGAAACA TTTAGAACTG AGAGATACTC ATTTGGACAC TTTCCACTTT 
TCAGAGGCAT CCATCAATGA AACACATACG TTGGTTAAAA AGTGGACATT TAGAAATGTG 
AAAGTCACCG ATAGAAGTTT TACTGAGGTT GTGAGACTGT TGAATTATGT TTCTGGAGTG 
TTAGAAGTAG AGTTTGAGGA CTGTACCCTT TATGGGCTCG GTGATTTTGA CATACCTGAT 
GTGGACAAAA TTAAAAATAT AGGTCAGATA GAGACACTAA CAGTACGGAG GTTGCATATT 
CCACACTTTT ACTCATTTTA CGATATGAGT AGTATATATT CACTTACAGA AGATGTTAAA 
AGAATCACAG TAGAAAGCAG TAAGGTCTTT CTGGTTCCTT GCTCACTTTC ACAACATTTA 
AAATCCCTAG AATATTTGGA TCTCAGCGAC AATTTAGTGG TTGAAGAATA CTTGAGAAAC 
TCAGCCTGTC AGCATGCTTG GCCCCTCCTG CAAACCTTAA TTTTAAGGCA AAATCGTTTG 
AAATCCTTAG AGAAAACTGG AGAAACTTTG CTTACTTTGA AAAACCTGGT GAACCTTGAT 
ATTAGTAAGA ATAATTATCT TTCTATGCCT GAAACTTGTC AGTGGCCAGA AAAGCTGAAA 
TGTTTGAACT TATCCGACAC AAGAATGCAA AGTATAACCC GTTGCATCCC TCAGACACTG 
GAAATTTTAG ATGTTAGCAA TAATAATCTC GAGTCATTTT CCCTGATTTT GCCACAACTT 
AAAGAACTTT CTATTTCCAG AAATAAGTTG AAGACTCTAC CAGATGCCTC CTTCTTACCC 
ACCTTACAAA TTATGAGAAT CAGCAGAAAC ACAATAAACG CTTTCTCGAA GGAGCAACTG 
GATTCCTTTC ACAGGCTGCA GACCCTGGAG GCCGGTGGCA ACAACTTCCT TTGCTCCTGT 
GAATTCCTGT CTTTCACTCA GGAGCAGCAG GCCCTGGCCG GGCTCCTGGT CGGCTGGCCA 
GAGGACTACC TGTGCCACTC CCCCTCCTAC GTGCGGGGCC AGCGGGTTGG GACCGCCCGG 
CTCCCGGCTT CTGAGTGCCA CCGGACAGCT CTGGTGGCCG CCGTGTGCTG TGTCTTGCTC 
CTGCTGGTCC TGCTCACGGC GGGGGCGTGC CACCATTTCC ACGGGCTGTG GTACCTGAGA 
ATGCTGTGGG CCTGGCTCCA GGCCAAAAGG AAGCCCAGGA AAGCCCCCTC CAGGGACGTC 
TGTTATGACG CCTTTGTGTC TTACAGTGAG CATGATTCCT ACTGGGTGGA GAACCTTCTG 
GTCCAGAAGC TGGAGCACTT CAATCCCCCG TTCAAGTTGT GCCTTCACAA GCGGGACTTT 
ATTCCCGGCA AGTGGATTAT TGACAATATC ATTGACTCCA TCGAGAAGAG CCACAAAACC 
ATCTTTGTGC TTTCTGAAAA CTTCGTGAAA AGCGAGTGGT GCAAGTACGA GCTGGACTTC 
TCCCATTTTC GCCTCTTTGA TGAGAACAAC GATGCTGCCA TCCTCATTCT TCTGGAGCCC 
ATTGAGAAAA AGGCCATCCC CCAGCGATTC TGTAAGCTGC GGAAGATAAT GAACACCAAG 
ACGTACCTGG AGTGGCCCAC CGATGATGCT CAGCAGGAAG GGTTTTGGTT AAATTTGAGA 
ACAGCAATAA AATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR2 ( XM_022427657.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022283365.1 Canis lupus familiaris toll like receptor 2 (TLR2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe17077
Clone ID Related Accession (Same CDS sequence)	XM_022427657.1 , XM_005628829.2 , XM_005628829.3
Accession Version	XM_022427657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	toll-like receptor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGTG TTTTGTGGAC ATTGTGGGTT TTGGGGGCTG TAACCAACCT CTCCAAGGAA 
GAGGCCCCTG ACCAGTCTTC TTCTCTGTCC TGTGACCCCA CTGGTGTCTG CGATGGCCGC 
TCCAGATCTT TGAACTCCAT GCCCTCAGGG CTCACAGCAG CTGTGAGAAG CCTTGACCTC 
TCCAACAATG AGATCACCTA CATTGGCAAC AGTGACCTTC GGGATTGTGT GAACCTCAAG 
GCTCTGAGGC TGGAGTCTAA TGGAATTAAC ACAATAGAGG AAGAATCTTT TTTTTCCCTG 
TGGAGTCTTG AACATTTGGA CTTATCTTAT AACCTCTTAT CTAACTTATC ATCCTCCTGG 
TTCAGGCCCC TTTCTTCATT GAAGTTCTTA AACCTACTGG GAAATCCTTA CAAATCACTT 
GGGGAAACAC CTCTTTTTTC TCAGCTCACA AATCTAAGAA TTCTGAAAGT AGGAAATATC 
TACAGCTTCA CTGAGATTCA GGATAAGGAT TTTGCTGGGC TAACCTTTCT TGAGGAACTG 
GAGATCGATG CTTCAAATCT CCAGAGGTAT GAGCCAAAGA GTTTGAAATC GATTCAGAAC 
ATCAGCTATC TGGCCCTCCG TATGAAGCAG CCTGTTTTAC TGGTGGAGAT TTTTGTAGAT 
CTTTCCAGTT CCTTGAAACA TTTAGAACTG AGAGATACTC ATTTGGACAC TTTCCACTTT 
TCAGAGGCAT CCATCAATGA AACACATACG TTGGTTAAAA AGTGGACATT TAGAAATGTG 
AAAGTCACCG ATAGAAGTTT TACTGAGGTT GTGAGACTGT TGAATTATGT TTCTGGAGTG 
TTAGAAGTAG AGTTTGAGGA CTGTACCCTT TATGGGCTCG GTGATTTTGA CATACCTGAT 
GTGGACAAAA TTAAAAATAT AGGTCAGATA GAGACACTAA CAGTACGGAG GTTGCATATT 
CCACACTTTT ACTCATTTTA CGATATGAGT AGTATATATT CACTTACAGA AGATGTTAAA 
AGAATCACAG TAGAAAGCAG TAAGGTCTTT CTGGTTCCTT GCTCACTTTC ACAACATTTA 
AAATCCCTAG AATATTTGGA TCTCAGCGAC AATTTAATGG TTGAAGAATA CTTGAGAAAC 
TCAGCCTGTC AGCATGCTTG GCCCCTCCTG CAAACCTTAA TTTTAAGGCA AAATCGTTTG 
AAATCCTTAG AGAAAACTGG AGAAACTTTG CTTACTTTGA AAAACCTGGT GAACCTTGAT 
ATTAGTAAGA ATAATTATCT TTCTATGCCT GAAACTTGTC AGTGGCCAGA AAAGCTGAAA 
TGTTTGAACT TATCCGACAC AAGAATGCAA AGTATAACCC GTTGCATCCC TCAGACACTG 
GAAATTTTAG ATGTTAGCAA TAATAATCTC GAGTCATTTT CCCTGATTTT GCCACAACTT 
AAAGAACTTT CTATTTCCAG AAATAAGTTG AAGACTCTAC CAGATGCCTC CTTCTTACCC 
ACCTTACAAA TTATGAGAAT CAGCAGAAAC ACAATAAACG CTTTCTCGAA GGAGCAACTG 
GATTCCTTTC ACAGGCTGCA GACCCTGGAG GCCGGTGGCA ACAACTTCCT TTGCTCCTGT 
GAATTCCTGT CTTTCACTCA GGAGCAGCAG GCCCTGGCCG GGCTCCTGGT CGGCTGGCCA 
GAGGACTACC TGTGCCACTC CCCCTCCTAC GTGCGGGGCC AGCGGGTTGG GACCGCCCGG 
CTCCCGGCTT CTGAGTGCCA CCGGACAGCT CTGGTGGCCG CCGTGTGCTG TGTCTTGCTC 
CTGCTGGTCC TGCTCACGGC GGGGGCGTGC CACCATTTCC ACGGGCTGTG GTACCTGAGA 
ATGCTGTGGG CCTGGCTCCA GGCCAAAAGG AAGCCCAGGA AAGCCCCCTC CAGGGACGTC 
TGTTATGACG CCTTTGTGTC TTACAGTGAG CATGATTCCT ACTGGGTGGA GAACCTTCTG 
GTCCAGAAGC TGGAGCACTT CAATCCCCCG TTCAAGTTGT GCCTTCACAA GCGGGACTTT 
ATTCCCGGCA AGTGGATTAT TGACAATATC ATTGACTCCA TCGAGAAGAG CCACAAAACC 
ATCTTTGTGC TTTCTGAAAA CTTCGTGAAG AGCGAGTGGT GCAAGTACGA GCTGGACTTC 
TCCCATTTTC GCCTCTTTGA TGAGAACAAC GATGCTGCCA TCCTCATTCT TCTGGAGCCC 
ATTGAGAAAA AGGCCATCCC CCAGCGATTC TGTAAGCTGC GGAAGATAAT GAACACCAAG 
ACGTACCTGG AGTGGCCCAC CGATGATGCT CAGCAGGAAG GGTTTTGGTT AAATTTGAGA 
ACAGCAATAA AATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022283365.1
CDS	79..2436
Translation	MSRVLWTLWVLGAVTNLSKEEAPDQSSSLSCDPTGVCDGRSRSLNSMPSGLTAAVRSLDLSNNEITYIGNSDLRDCVNLKALRLESNGINTIEEESFFSLWSLEHLDLSYNLLSNLSSSWFRPLSSLKFLNLLGNPYKSLGETPLFSQLTNLRILKVGNIYSFTEIQDKDFAGLTFLEELEIDASNLQRYEPKSLKSIQNISYLALRMKQPVLLVEIFVDLSSSLKHLELRDTHLDTFHFSEASINETHTLVKKWTFRNVKVTDRSFTEVVRLLNYVSGVLEVEFEDCTLYGLGDFDIPDVDKIKNIGQIETLTVRRLHIPHFYSFYDMSSIYSLTEDVKRITVESSKVFLVPCSLSQHLKSLEYLDLSDNLMVEEYLRNSACQHAWPLLQTLILRQNRLKSLEKTGETLLTLKNLVNLDISKNNYLSMPETCQWPEKLKCLNLSDTRMQSITRCIPQTLEILDVSNNNLESFSLILPQLKELSISRNKLKTLPDASFLPTLQIMRISRNTINAFSKEQLDSFHRLQTLEAGGNNFLCSCEFLSFTQEQQALAGLLVGWPEDYLCHSPSYVRGQRVGTARLPASECHRTALVAAVCCVLLLLVLLTAGACHHFHGLWYLRMLWAWLQAKRKPRKAPSRDVCYDAFVSYSEHDSYWVENLLVQKLEHFNPPFKLCLHKRDFIPGKWIIDNIIDSIEKSHKTIFVLSENFVKSEWCKYELDFSHFRLFDENNDAAILILLEPIEKKAIPQRFCKLRKIMNTKTYLEWPTDDAQQEGFWLNLRTAIKS

Target ORF information:

RefSeq Version	XM_022427657.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris toll like receptor 2 (TLR2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022427657.1

ORF Insert Sequence:

ATGTCACGTG TTTTGTGGAC ATTGTGGGTT TTGGGGGCTG TAACCAACCT CTCCAAGGAA 
GAGGCCCCTG ACCAGTCTTC TTCTCTGTCC TGTGACCCCA CTGGTGTCTG CGATGGCCGC 
TCCAGATCTT TGAACTCCAT GCCCTCAGGG CTCACAGCAG CTGTGAGAAG CCTTGACCTC 
TCCAACAATG AGATCACCTA CATTGGCAAC AGTGACCTTC GGGATTGTGT GAACCTCAAG 
GCTCTGAGGC TGGAGTCTAA TGGAATTAAC ACAATAGAGG AAGAATCTTT TTTTTCCCTG 
TGGAGTCTTG AACATTTGGA CTTATCTTAT AACCTCTTAT CTAACTTATC ATCCTCCTGG 
TTCAGGCCCC TTTCTTCATT GAAGTTCTTA AACCTACTGG GAAATCCTTA CAAATCACTT 
GGGGAAACAC CTCTTTTTTC TCAGCTCACA AATCTAAGAA TTCTGAAAGT AGGAAATATC 
TACAGCTTCA CTGAGATTCA GGATAAGGAT TTTGCTGGGC TAACCTTTCT TGAGGAACTG 
GAGATCGATG CTTCAAATCT CCAGAGGTAT GAGCCAAAGA GTTTGAAATC GATTCAGAAC 
ATCAGCTATC TGGCCCTCCG TATGAAGCAG CCTGTTTTAC TGGTGGAGAT TTTTGTAGAT 
CTTTCCAGTT CCTTGAAACA TTTAGAACTG AGAGATACTC ATTTGGACAC TTTCCACTTT 
TCAGAGGCAT CCATCAATGA AACACATACG TTGGTTAAAA AGTGGACATT TAGAAATGTG 
AAAGTCACCG ATAGAAGTTT TACTGAGGTT GTGAGACTGT TGAATTATGT TTCTGGAGTG 
TTAGAAGTAG AGTTTGAGGA CTGTACCCTT TATGGGCTCG GTGATTTTGA CATACCTGAT 
GTGGACAAAA TTAAAAATAT AGGTCAGATA GAGACACTAA CAGTACGGAG GTTGCATATT 
CCACACTTTT ACTCATTTTA CGATATGAGT AGTATATATT CACTTACAGA AGATGTTAAA 
AGAATCACAG TAGAAAGCAG TAAGGTCTTT CTGGTTCCTT GCTCACTTTC ACAACATTTA 
AAATCCCTAG AATATTTGGA TCTCAGCGAC AATTTAATGG TTGAAGAATA CTTGAGAAAC 
TCAGCCTGTC AGCATGCTTG GCCCCTCCTG CAAACCTTAA TTTTAAGGCA AAATCGTTTG 
AAATCCTTAG AGAAAACTGG AGAAACTTTG CTTACTTTGA AAAACCTGGT GAACCTTGAT 
ATTAGTAAGA ATAATTATCT TTCTATGCCT GAAACTTGTC AGTGGCCAGA AAAGCTGAAA 
TGTTTGAACT TATCCGACAC AAGAATGCAA AGTATAACCC GTTGCATCCC TCAGACACTG 
GAAATTTTAG ATGTTAGCAA TAATAATCTC GAGTCATTTT CCCTGATTTT GCCACAACTT 
AAAGAACTTT CTATTTCCAG AAATAAGTTG AAGACTCTAC CAGATGCCTC CTTCTTACCC 
ACCTTACAAA TTATGAGAAT CAGCAGAAAC ACAATAAACG CTTTCTCGAA GGAGCAACTG 
GATTCCTTTC ACAGGCTGCA GACCCTGGAG GCCGGTGGCA ACAACTTCCT TTGCTCCTGT 
GAATTCCTGT CTTTCACTCA GGAGCAGCAG GCCCTGGCCG GGCTCCTGGT CGGCTGGCCA 
GAGGACTACC TGTGCCACTC CCCCTCCTAC GTGCGGGGCC AGCGGGTTGG GACCGCCCGG 
CTCCCGGCTT CTGAGTGCCA CCGGACAGCT CTGGTGGCCG CCGTGTGCTG TGTCTTGCTC 
CTGCTGGTCC TGCTCACGGC GGGGGCGTGC CACCATTTCC ACGGGCTGTG GTACCTGAGA 
ATGCTGTGGG CCTGGCTCCA GGCCAAAAGG AAGCCCAGGA AAGCCCCCTC CAGGGACGTC 
TGTTATGACG CCTTTGTGTC TTACAGTGAG CATGATTCCT ACTGGGTGGA GAACCTTCTG 
GTCCAGAAGC TGGAGCACTT CAATCCCCCG TTCAAGTTGT GCCTTCACAA GCGGGACTTT 
ATTCCCGGCA AGTGGATTAT TGACAATATC ATTGACTCCA TCGAGAAGAG CCACAAAACC 
ATCTTTGTGC TTTCTGAAAA CTTCGTGAAG AGCGAGTGGT GCAAGTACGA GCTGGACTTC 
TCCCATTTTC GCCTCTTTGA TGAGAACAAC GATGCTGCCA TCCTCATTCT TCTGGAGCCC 
ATTGAGAAAA AGGCCATCCC CCAGCGATTC TGTAAGCTGC GGAAGATAAT GAACACCAAG 
ACGTACCTGG AGTGGCCCAC CGATGATGCT CAGCAGGAAG GGTTTTGGTT AAATTTGAGA 
ACAGCAATAA AATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR2 ( XM_005628829.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005628886.1 Canis lupus familiaris toll-like receptor 2 (TLR2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe17077
Clone ID Related Accession (Same CDS sequence)	XM_022427657.1 , XM_005628829.2 , XM_005628829.3
Accession Version	XM_005628829.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	toll-like receptor 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005628829.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGTG TTTTGTGGAC ATTGTGGGTT TTGGGGGCTG TAACCAACCT CTCCAAGGAA 
GAGGCCCCTG ACCAGTCTTC TTCTCTGTCC TGTGACCCCA CTGGTGTCTG CGATGGCCGC 
TCCAGATCTT TGAACTCCAT GCCCTCAGGG CTCACAGCAG CTGTGAGAAG CCTTGACCTC 
TCCAACAATG AGATCACCTA CATTGGCAAC AGTGACCTTC GGGATTGTGT GAACCTCAAG 
GCTCTGAGGC TGGAGTCTAA TGGAATTAAC ACAATAGAGG AAGAATCTTT TTTTTCCCTG 
TGGAGTCTTG AACATTTGGA CTTATCTTAT AACCTCTTAT CTAACTTATC ATCCTCCTGG 
TTCAGGCCCC TTTCTTCATT GAAGTTCTTA AACCTACTGG GAAATCCTTA CAAATCACTT 
GGGGAAACAC CTCTTTTTTC TCAGCTCACA AATCTAAGAA TTCTGAAAGT AGGAAATATC 
TACAGCTTCA CTGAGATTCA GGATAAGGAT TTTGCTGGGC TAACCTTTCT TGAGGAACTG 
GAGATCGATG CTTCAAATCT CCAGAGGTAT GAGCCAAAGA GTTTGAAATC GATTCAGAAC 
ATCAGCTATC TGGCCCTCCG TATGAAGCAG CCTGTTTTAC TGGTGGAGAT TTTTGTAGAT 
CTTTCCAGTT CCTTGAAACA TTTAGAACTG AGAGATACTC ATTTGGACAC TTTCCACTTT 
TCAGAGGCAT CCATCAATGA AACACATACG TTGGTTAAAA AGTGGACATT TAGAAATGTG 
AAAGTCACCG ATAGAAGTTT TACTGAGGTT GTGAGACTGT TGAATTATGT TTCTGGAGTG 
TTAGAAGTAG AGTTTGAGGA CTGTACCCTT TATGGGCTCG GTGATTTTGA CATACCTGAT 
GTGGACAAAA TTAAAAATAT AGGTCAGATA GAGACACTAA CAGTACGGAG GTTGCATATT 
CCACACTTTT ACTCATTTTA CGATATGAGT AGTATATATT CACTTACAGA AGATGTTAAA 
AGAATCACAG TAGAAAGCAG TAAGGTCTTT CTGGTTCCTT GCTCACTTTC ACAACATTTA 
AAATCCCTAG AATATTTGGA TCTCAGCGAC AATTTAATGG TTGAAGAATA CTTGAGAAAC 
TCAGCCTGTC AGCATGCTTG GCCCCTCCTG CAAACCTTAA TTTTAAGGCA AAATCGTTTG 
AAATCCTTAG AGAAAACTGG AGAAACTTTG CTTACTTTGA AAAACCTGGT GAACCTTGAT 
ATTAGTAAGA ATAATTATCT TTCTATGCCT GAAACTTGTC AGTGGCCAGA AAAGCTGAAA 
TGTTTGAACT TATCCGACAC AAGAATGCAA AGTATAACCC GTTGCATCCC TCAGACACTG 
GAAATTTTAG ATGTTAGCAA TAATAATCTC GAGTCATTTT CCCTGATTTT GCCACAACTT 
AAAGAACTTT CTATTTCCAG AAATAAGTTG AAGACTCTAC CAGATGCCTC CTTCTTACCC 
ACCTTACAAA TTATGAGAAT CAGCAGAAAC ACAATAAACG CTTTCTCGAA GGAGCAACTG 
GATTCCTTTC ACAGGCTGCA GACCCTGGAG GCCGGTGGCA ACAACTTCCT TTGCTCCTGT 
GAATTCCTGT CTTTCACTCA GGAGCAGCAG GCCCTGGCCG GGCTCCTGGT CGGCTGGCCA 
GAGGACTACC TGTGCCACTC CCCCTCCTAC GTGCGGGGCC AGCGGGTTGG GACCGCCCGG 
CTCCCGGCTT CTGAGTGCCA CCGGACAGCT CTGGTGGCCG CCGTGTGCTG TGTCTTGCTC 
CTGCTGGTCC TGCTCACGGC GGGGGCGTGC CACCATTTCC ACGGGCTGTG GTACCTGAGA 
ATGCTGTGGG CCTGGCTCCA GGCCAAAAGG AAGCCCAGGA AAGCCCCCTC CAGGGACGTC 
TGTTATGACG CCTTTGTGTC TTACAGTGAG CATGATTCCT ACTGGGTGGA GAACCTTCTG 
GTCCAGAAGC TGGAGCACTT CAATCCCCCG TTCAAGTTGT GCCTTCACAA GCGGGACTTT 
ATTCCCGGCA AGTGGATTAT TGACAATATC ATTGACTCCA TCGAGAAGAG CCACAAAACC 
ATCTTTGTGC TTTCTGAAAA CTTCGTGAAG AGCGAGTGGT GCAAGTACGA GCTGGACTTC 
TCCCATTTTC GCCTCTTTGA TGAGAACAAC GATGCTGCCA TCCTCATTCT TCTGGAGCCC 
ATTGAGAAAA AGGCCATCCC CCAGCGATTC TGTAAGCTGC GGAAGATAAT GAACACCAAG 
ACGTACCTGG AGTGGCCCAC CGATGATGCT CAGCAGGAAG GGTTTTGGTT AAATTTGAGA 
ACAGCAATAA AATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005628886.1
CDS	51..2408
Translation	MSRVLWTLWVLGAVTNLSKEEAPDQSSSLSCDPTGVCDGRSRSLNSMPSGLTAAVRSLDLSNNEITYIGNSDLRDCVNLKALRLESNGINTIEEESFFSLWSLEHLDLSYNLLSNLSSSWFRPLSSLKFLNLLGNPYKSLGETPLFSQLTNLRILKVGNIYSFTEIQDKDFAGLTFLEELEIDASNLQRYEPKSLKSIQNISYLALRMKQPVLLVEIFVDLSSSLKHLELRDTHLDTFHFSEASINETHTLVKKWTFRNVKVTDRSFTEVVRLLNYVSGVLEVEFEDCTLYGLGDFDIPDVDKIKNIGQIETLTVRRLHIPHFYSFYDMSSIYSLTEDVKRITVESSKVFLVPCSLSQHLKSLEYLDLSDNLMVEEYLRNSACQHAWPLLQTLILRQNRLKSLEKTGETLLTLKNLVNLDISKNNYLSMPETCQWPEKLKCLNLSDTRMQSITRCIPQTLEILDVSNNNLESFSLILPQLKELSISRNKLKTLPDASFLPTLQIMRISRNTINAFSKEQLDSFHRLQTLEAGGNNFLCSCEFLSFTQEQQALAGLLVGWPEDYLCHSPSYVRGQRVGTARLPASECHRTALVAAVCCVLLLLVLLTAGACHHFHGLWYLRMLWAWLQAKRKPRKAPSRDVCYDAFVSYSEHDSYWVENLLVQKLEHFNPPFKLCLHKRDFIPGKWIIDNIIDSIEKSHKTIFVLSENFVKSEWCKYELDFSHFRLFDENNDAAILILLEPIEKKAIPQRFCKLRKIMNTKTYLEWPTDDAQQEGFWLNLRTAIKS

Target ORF information:

RefSeq Version	XM_005628829.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris toll-like receptor 2 (TLR2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005628829.2

ORF Insert Sequence:

ATGTCACGTG TTTTGTGGAC ATTGTGGGTT TTGGGGGCTG TAACCAACCT CTCCAAGGAA 
GAGGCCCCTG ACCAGTCTTC TTCTCTGTCC TGTGACCCCA CTGGTGTCTG CGATGGCCGC 
TCCAGATCTT TGAACTCCAT GCCCTCAGGG CTCACAGCAG CTGTGAGAAG CCTTGACCTC 
TCCAACAATG AGATCACCTA CATTGGCAAC AGTGACCTTC GGGATTGTGT GAACCTCAAG 
GCTCTGAGGC TGGAGTCTAA TGGAATTAAC ACAATAGAGG AAGAATCTTT TTTTTCCCTG 
TGGAGTCTTG AACATTTGGA CTTATCTTAT AACCTCTTAT CTAACTTATC ATCCTCCTGG 
TTCAGGCCCC TTTCTTCATT GAAGTTCTTA AACCTACTGG GAAATCCTTA CAAATCACTT 
GGGGAAACAC CTCTTTTTTC TCAGCTCACA AATCTAAGAA TTCTGAAAGT AGGAAATATC 
TACAGCTTCA CTGAGATTCA GGATAAGGAT TTTGCTGGGC TAACCTTTCT TGAGGAACTG 
GAGATCGATG CTTCAAATCT CCAGAGGTAT GAGCCAAAGA GTTTGAAATC GATTCAGAAC 
ATCAGCTATC TGGCCCTCCG TATGAAGCAG CCTGTTTTAC TGGTGGAGAT TTTTGTAGAT 
CTTTCCAGTT CCTTGAAACA TTTAGAACTG AGAGATACTC ATTTGGACAC TTTCCACTTT 
TCAGAGGCAT CCATCAATGA AACACATACG TTGGTTAAAA AGTGGACATT TAGAAATGTG 
AAAGTCACCG ATAGAAGTTT TACTGAGGTT GTGAGACTGT TGAATTATGT TTCTGGAGTG 
TTAGAAGTAG AGTTTGAGGA CTGTACCCTT TATGGGCTCG GTGATTTTGA CATACCTGAT 
GTGGACAAAA TTAAAAATAT AGGTCAGATA GAGACACTAA CAGTACGGAG GTTGCATATT 
CCACACTTTT ACTCATTTTA CGATATGAGT AGTATATATT CACTTACAGA AGATGTTAAA 
AGAATCACAG TAGAAAGCAG TAAGGTCTTT CTGGTTCCTT GCTCACTTTC ACAACATTTA 
AAATCCCTAG AATATTTGGA TCTCAGCGAC AATTTAATGG TTGAAGAATA CTTGAGAAAC 
TCAGCCTGTC AGCATGCTTG GCCCCTCCTG CAAACCTTAA TTTTAAGGCA AAATCGTTTG 
AAATCCTTAG AGAAAACTGG AGAAACTTTG CTTACTTTGA AAAACCTGGT GAACCTTGAT 
ATTAGTAAGA ATAATTATCT TTCTATGCCT GAAACTTGTC AGTGGCCAGA AAAGCTGAAA 
TGTTTGAACT TATCCGACAC AAGAATGCAA AGTATAACCC GTTGCATCCC TCAGACACTG 
GAAATTTTAG ATGTTAGCAA TAATAATCTC GAGTCATTTT CCCTGATTTT GCCACAACTT 
AAAGAACTTT CTATTTCCAG AAATAAGTTG AAGACTCTAC CAGATGCCTC CTTCTTACCC 
ACCTTACAAA TTATGAGAAT CAGCAGAAAC ACAATAAACG CTTTCTCGAA GGAGCAACTG 
GATTCCTTTC ACAGGCTGCA GACCCTGGAG GCCGGTGGCA ACAACTTCCT TTGCTCCTGT 
GAATTCCTGT CTTTCACTCA GGAGCAGCAG GCCCTGGCCG GGCTCCTGGT CGGCTGGCCA 
GAGGACTACC TGTGCCACTC CCCCTCCTAC GTGCGGGGCC AGCGGGTTGG GACCGCCCGG 
CTCCCGGCTT CTGAGTGCCA CCGGACAGCT CTGGTGGCCG CCGTGTGCTG TGTCTTGCTC 
CTGCTGGTCC TGCTCACGGC GGGGGCGTGC CACCATTTCC ACGGGCTGTG GTACCTGAGA 
ATGCTGTGGG CCTGGCTCCA GGCCAAAAGG AAGCCCAGGA AAGCCCCCTC CAGGGACGTC 
TGTTATGACG CCTTTGTGTC TTACAGTGAG CATGATTCCT ACTGGGTGGA GAACCTTCTG 
GTCCAGAAGC TGGAGCACTT CAATCCCCCG TTCAAGTTGT GCCTTCACAA GCGGGACTTT 
ATTCCCGGCA AGTGGATTAT TGACAATATC ATTGACTCCA TCGAGAAGAG CCACAAAACC 
ATCTTTGTGC TTTCTGAAAA CTTCGTGAAG AGCGAGTGGT GCAAGTACGA GCTGGACTTC 
TCCCATTTTC GCCTCTTTGA TGAGAACAAC GATGCTGCCA TCCTCATTCT TCTGGAGCCC 
ATTGAGAAAA AGGCCATCCC CCAGCGATTC TGTAAGCTGC GGAAGATAAT GAACACCAAG 
ACGTACCTGG AGTGGCCCAC CGATGATGCT CAGCAGGAAG GGTTTTGGTT AAATTTGAGA 
ACAGCAATAA AATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR2 ( XM_005628829.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005628886.1 Canis lupus familiaris toll like receptor 2 (TLR2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe17077
Clone ID Related Accession (Same CDS sequence)	XM_022427657.1 , XM_005628829.2 , XM_005628829.3
Accession Version	XM_005628829.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	toll-like receptor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005628829.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGTG TTTTGTGGAC ATTGTGGGTT TTGGGGGCTG TAACCAACCT CTCCAAGGAA 
GAGGCCCCTG ACCAGTCTTC TTCTCTGTCC TGTGACCCCA CTGGTGTCTG CGATGGCCGC 
TCCAGATCTT TGAACTCCAT GCCCTCAGGG CTCACAGCAG CTGTGAGAAG CCTTGACCTC 
TCCAACAATG AGATCACCTA CATTGGCAAC AGTGACCTTC GGGATTGTGT GAACCTCAAG 
GCTCTGAGGC TGGAGTCTAA TGGAATTAAC ACAATAGAGG AAGAATCTTT TTTTTCCCTG 
TGGAGTCTTG AACATTTGGA CTTATCTTAT AACCTCTTAT CTAACTTATC ATCCTCCTGG 
TTCAGGCCCC TTTCTTCATT GAAGTTCTTA AACCTACTGG GAAATCCTTA CAAATCACTT 
GGGGAAACAC CTCTTTTTTC TCAGCTCACA AATCTAAGAA TTCTGAAAGT AGGAAATATC 
TACAGCTTCA CTGAGATTCA GGATAAGGAT TTTGCTGGGC TAACCTTTCT TGAGGAACTG 
GAGATCGATG CTTCAAATCT CCAGAGGTAT GAGCCAAAGA GTTTGAAATC GATTCAGAAC 
ATCAGCTATC TGGCCCTCCG TATGAAGCAG CCTGTTTTAC TGGTGGAGAT TTTTGTAGAT 
CTTTCCAGTT CCTTGAAACA TTTAGAACTG AGAGATACTC ATTTGGACAC TTTCCACTTT 
TCAGAGGCAT CCATCAATGA AACACATACG TTGGTTAAAA AGTGGACATT TAGAAATGTG 
AAAGTCACCG ATAGAAGTTT TACTGAGGTT GTGAGACTGT TGAATTATGT TTCTGGAGTG 
TTAGAAGTAG AGTTTGAGGA CTGTACCCTT TATGGGCTCG GTGATTTTGA CATACCTGAT 
GTGGACAAAA TTAAAAATAT AGGTCAGATA GAGACACTAA CAGTACGGAG GTTGCATATT 
CCACACTTTT ACTCATTTTA CGATATGAGT AGTATATATT CACTTACAGA AGATGTTAAA 
AGAATCACAG TAGAAAGCAG TAAGGTCTTT CTGGTTCCTT GCTCACTTTC ACAACATTTA 
AAATCCCTAG AATATTTGGA TCTCAGCGAC AATTTAATGG TTGAAGAATA CTTGAGAAAC 
TCAGCCTGTC AGCATGCTTG GCCCCTCCTG CAAACCTTAA TTTTAAGGCA AAATCGTTTG 
AAATCCTTAG AGAAAACTGG AGAAACTTTG CTTACTTTGA AAAACCTGGT GAACCTTGAT 
ATTAGTAAGA ATAATTATCT TTCTATGCCT GAAACTTGTC AGTGGCCAGA AAAGCTGAAA 
TGTTTGAACT TATCCGACAC AAGAATGCAA AGTATAACCC GTTGCATCCC TCAGACACTG 
GAAATTTTAG ATGTTAGCAA TAATAATCTC GAGTCATTTT CCCTGATTTT GCCACAACTT 
AAAGAACTTT CTATTTCCAG AAATAAGTTG AAGACTCTAC CAGATGCCTC CTTCTTACCC 
ACCTTACAAA TTATGAGAAT CAGCAGAAAC ACAATAAACG CTTTCTCGAA GGAGCAACTG 
GATTCCTTTC ACAGGCTGCA GACCCTGGAG GCCGGTGGCA ACAACTTCCT TTGCTCCTGT 
GAATTCCTGT CTTTCACTCA GGAGCAGCAG GCCCTGGCCG GGCTCCTGGT CGGCTGGCCA 
GAGGACTACC TGTGCCACTC CCCCTCCTAC GTGCGGGGCC AGCGGGTTGG GACCGCCCGG 
CTCCCGGCTT CTGAGTGCCA CCGGACAGCT CTGGTGGCCG CCGTGTGCTG TGTCTTGCTC 
CTGCTGGTCC TGCTCACGGC GGGGGCGTGC CACCATTTCC ACGGGCTGTG GTACCTGAGA 
ATGCTGTGGG CCTGGCTCCA GGCCAAAAGG AAGCCCAGGA AAGCCCCCTC CAGGGACGTC 
TGTTATGACG CCTTTGTGTC TTACAGTGAG CATGATTCCT ACTGGGTGGA GAACCTTCTG 
GTCCAGAAGC TGGAGCACTT CAATCCCCCG TTCAAGTTGT GCCTTCACAA GCGGGACTTT 
ATTCCCGGCA AGTGGATTAT TGACAATATC ATTGACTCCA TCGAGAAGAG CCACAAAACC 
ATCTTTGTGC TTTCTGAAAA CTTCGTGAAG AGCGAGTGGT GCAAGTACGA GCTGGACTTC 
TCCCATTTTC GCCTCTTTGA TGAGAACAAC GATGCTGCCA TCCTCATTCT TCTGGAGCCC 
ATTGAGAAAA AGGCCATCCC CCAGCGATTC TGTAAGCTGC GGAAGATAAT GAACACCAAG 
ACGTACCTGG AGTGGCCCAC CGATGATGCT CAGCAGGAAG GGTTTTGGTT AAATTTGAGA 
ACAGCAATAA AATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005628886.1
CDS	51..2408
Translation	MSRVLWTLWVLGAVTNLSKEEAPDQSSSLSCDPTGVCDGRSRSLNSMPSGLTAAVRSLDLSNNEITYIGNSDLRDCVNLKALRLESNGINTIEEESFFSLWSLEHLDLSYNLLSNLSSSWFRPLSSLKFLNLLGNPYKSLGETPLFSQLTNLRILKVGNIYSFTEIQDKDFAGLTFLEELEIDASNLQRYEPKSLKSIQNISYLALRMKQPVLLVEIFVDLSSSLKHLELRDTHLDTFHFSEASINETHTLVKKWTFRNVKVTDRSFTEVVRLLNYVSGVLEVEFEDCTLYGLGDFDIPDVDKIKNIGQIETLTVRRLHIPHFYSFYDMSSIYSLTEDVKRITVESSKVFLVPCSLSQHLKSLEYLDLSDNLMVEEYLRNSACQHAWPLLQTLILRQNRLKSLEKTGETLLTLKNLVNLDISKNNYLSMPETCQWPEKLKCLNLSDTRMQSITRCIPQTLEILDVSNNNLESFSLILPQLKELSISRNKLKTLPDASFLPTLQIMRISRNTINAFSKEQLDSFHRLQTLEAGGNNFLCSCEFLSFTQEQQALAGLLVGWPEDYLCHSPSYVRGQRVGTARLPASECHRTALVAAVCCVLLLLVLLTAGACHHFHGLWYLRMLWAWLQAKRKPRKAPSRDVCYDAFVSYSEHDSYWVENLLVQKLEHFNPPFKLCLHKRDFIPGKWIIDNIIDSIEKSHKTIFVLSENFVKSEWCKYELDFSHFRLFDENNDAAILILLEPIEKKAIPQRFCKLRKIMNTKTYLEWPTDDAQQEGFWLNLRTAIKS

Target ORF information:

RefSeq Version	XM_005628829.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris toll like receptor 2 (TLR2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005628829.3

ORF Insert Sequence:

ATGTCACGTG TTTTGTGGAC ATTGTGGGTT TTGGGGGCTG TAACCAACCT CTCCAAGGAA 
GAGGCCCCTG ACCAGTCTTC TTCTCTGTCC TGTGACCCCA CTGGTGTCTG CGATGGCCGC 
TCCAGATCTT TGAACTCCAT GCCCTCAGGG CTCACAGCAG CTGTGAGAAG CCTTGACCTC 
TCCAACAATG AGATCACCTA CATTGGCAAC AGTGACCTTC GGGATTGTGT GAACCTCAAG 
GCTCTGAGGC TGGAGTCTAA TGGAATTAAC ACAATAGAGG AAGAATCTTT TTTTTCCCTG 
TGGAGTCTTG AACATTTGGA CTTATCTTAT AACCTCTTAT CTAACTTATC ATCCTCCTGG 
TTCAGGCCCC TTTCTTCATT GAAGTTCTTA AACCTACTGG GAAATCCTTA CAAATCACTT 
GGGGAAACAC CTCTTTTTTC TCAGCTCACA AATCTAAGAA TTCTGAAAGT AGGAAATATC 
TACAGCTTCA CTGAGATTCA GGATAAGGAT TTTGCTGGGC TAACCTTTCT TGAGGAACTG 
GAGATCGATG CTTCAAATCT CCAGAGGTAT GAGCCAAAGA GTTTGAAATC GATTCAGAAC 
ATCAGCTATC TGGCCCTCCG TATGAAGCAG CCTGTTTTAC TGGTGGAGAT TTTTGTAGAT 
CTTTCCAGTT CCTTGAAACA TTTAGAACTG AGAGATACTC ATTTGGACAC TTTCCACTTT 
TCAGAGGCAT CCATCAATGA AACACATACG TTGGTTAAAA AGTGGACATT TAGAAATGTG 
AAAGTCACCG ATAGAAGTTT TACTGAGGTT GTGAGACTGT TGAATTATGT TTCTGGAGTG 
TTAGAAGTAG AGTTTGAGGA CTGTACCCTT TATGGGCTCG GTGATTTTGA CATACCTGAT 
GTGGACAAAA TTAAAAATAT AGGTCAGATA GAGACACTAA CAGTACGGAG GTTGCATATT 
CCACACTTTT ACTCATTTTA CGATATGAGT AGTATATATT CACTTACAGA AGATGTTAAA 
AGAATCACAG TAGAAAGCAG TAAGGTCTTT CTGGTTCCTT GCTCACTTTC ACAACATTTA 
AAATCCCTAG AATATTTGGA TCTCAGCGAC AATTTAATGG TTGAAGAATA CTTGAGAAAC 
TCAGCCTGTC AGCATGCTTG GCCCCTCCTG CAAACCTTAA TTTTAAGGCA AAATCGTTTG 
AAATCCTTAG AGAAAACTGG AGAAACTTTG CTTACTTTGA AAAACCTGGT GAACCTTGAT 
ATTAGTAAGA ATAATTATCT TTCTATGCCT GAAACTTGTC AGTGGCCAGA AAAGCTGAAA 
TGTTTGAACT TATCCGACAC AAGAATGCAA AGTATAACCC GTTGCATCCC TCAGACACTG 
GAAATTTTAG ATGTTAGCAA TAATAATCTC GAGTCATTTT CCCTGATTTT GCCACAACTT 
AAAGAACTTT CTATTTCCAG AAATAAGTTG AAGACTCTAC CAGATGCCTC CTTCTTACCC 
ACCTTACAAA TTATGAGAAT CAGCAGAAAC ACAATAAACG CTTTCTCGAA GGAGCAACTG 
GATTCCTTTC ACAGGCTGCA GACCCTGGAG GCCGGTGGCA ACAACTTCCT TTGCTCCTGT 
GAATTCCTGT CTTTCACTCA GGAGCAGCAG GCCCTGGCCG GGCTCCTGGT CGGCTGGCCA 
GAGGACTACC TGTGCCACTC CCCCTCCTAC GTGCGGGGCC AGCGGGTTGG GACCGCCCGG 
CTCCCGGCTT CTGAGTGCCA CCGGACAGCT CTGGTGGCCG CCGTGTGCTG TGTCTTGCTC 
CTGCTGGTCC TGCTCACGGC GGGGGCGTGC CACCATTTCC ACGGGCTGTG GTACCTGAGA 
ATGCTGTGGG CCTGGCTCCA GGCCAAAAGG AAGCCCAGGA AAGCCCCCTC CAGGGACGTC 
TGTTATGACG CCTTTGTGTC TTACAGTGAG CATGATTCCT ACTGGGTGGA GAACCTTCTG 
GTCCAGAAGC TGGAGCACTT CAATCCCCCG TTCAAGTTGT GCCTTCACAA GCGGGACTTT 
ATTCCCGGCA AGTGGATTAT TGACAATATC ATTGACTCCA TCGAGAAGAG CCACAAAACC 
ATCTTTGTGC TTTCTGAAAA CTTCGTGAAG AGCGAGTGGT GCAAGTACGA GCTGGACTTC 
TCCCATTTTC GCCTCTTTGA TGAGAACAAC GATGCTGCCA TCCTCATTCT TCTGGAGCCC 
ATTGAGAAAA AGGCCATCCC CCAGCGATTC TGTAAGCTGC GGAAGATAAT GAACACCAAG 
ACGTACCTGG AGTGGCCCAC CGATGATGCT CAGCAGGAAG GGTTTTGGTT AAATTTGAGA 
ACAGCAATAA AATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR2 ( XM_022427656.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022283364.1 Canis lupus familiaris toll like receptor 2 (TLR2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe293351
Clone ID Related Accession (Same CDS sequence)	XM_022427656.1
Accession Version	XM_022427656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	toll-like receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGGGA ACGACCGGGA AGGAGCCGAC GCAAGGTTGG ATCACTGGAC AATGTCACGT 
GTTTTGTGGA CATTGTGGGT TTTGGGGGCT GTAACCAACC TCTCCAAGGA AGAGGCCCCT 
GACCAGTCTT CTTCTCTGTC CTGTGACCCC ACTGGTGTCT GCGATGGCCG CTCCAGATCT 
TTGAACTCCA TGCCCTCAGG GCTCACAGCA GCTGTGAGAA GCCTTGACCT CTCCAACAAT 
GAGATCACCT ACATTGGCAA CAGTGACCTT CGGGATTGTG TGAACCTCAA GGCTCTGAGG 
CTGGAGTCTA ATGGAATTAA CACAATAGAG GAAGAATCTT TTTTTTCCCT GTGGAGTCTT 
GAACATTTGG ACTTATCTTA TAACCTCTTA TCTAACTTAT CATCCTCCTG GTTCAGGCCC 
CTTTCTTCAT TGAAGTTCTT AAACCTACTG GGAAATCCTT ACAAATCACT TGGGGAAACA 
CCTCTTTTTT CTCAGCTCAC AAATCTAAGA ATTCTGAAAG TAGGAAATAT CTACAGCTTC 
ACTGAGATTC AGGATAAGGA TTTTGCTGGG CTAACCTTTC TTGAGGAACT GGAGATCGAT 
GCTTCAAATC TCCAGAGGTA TGAGCCAAAG AGTTTGAAAT CGATTCAGAA CATCAGCTAT 
CTGGCCCTCC GTATGAAGCA GCCTGTTTTA CTGGTGGAGA TTTTTGTAGA TCTTTCCAGT 
TCCTTGAAAC ATTTAGAACT GAGAGATACT CATTTGGACA CTTTCCACTT TTCAGAGGCA 
TCCATCAATG AAACACATAC GTTGGTTAAA AAGTGGACAT TTAGAAATGT GAAAGTCACC 
GATAGAAGTT TTACTGAGGT TGTGAGACTG TTGAATTATG TTTCTGGAGT GTTAGAAGTA 
GAGTTTGAGG ACTGTACCCT TTATGGGCTC GGTGATTTTG ACATACCTGA TGTGGACAAA 
ATTAAAAATA TAGGTCAGAT AGAGACACTA ACAGTACGGA GGTTGCATAT TCCACACTTT 
TACTCATTTT ACGATATGAG TAGTATATAT TCACTTACAG AAGATGTTAA AAGAATCACA 
GTAGAAAGCA GTAAGGTCTT TCTGGTTCCT TGCTCACTTT CACAACATTT AAAATCCCTA 
GAATATTTGG ATCTCAGCGA CAATTTAATG GTTGAAGAAT ACTTGAGAAA CTCAGCCTGT 
CAGCATGCTT GGCCCCTCCT GCAAACCTTA ATTTTAAGGC AAAATCGTTT GAAATCCTTA 
GAGAAAACTG GAGAAACTTT GCTTACTTTG AAAAACCTGG TGAACCTTGA TATTAGTAAG 
AATAATTATC TTTCTATGCC TGAAACTTGT CAGTGGCCAG AAAAGCTGAA ATGTTTGAAC 
TTATCCGACA CAAGAATGCA AAGTATAACC CGTTGCATCC CTCAGACACT GGAAATTTTA 
GATGTTAGCA ATAATAATCT CGAGTCATTT TCCCTGATTT TGCCACAACT TAAAGAACTT 
TCTATTTCCA GAAATAAGTT GAAGACTCTA CCAGATGCCT CCTTCTTACC CACCTTACAA 
ATTATGAGAA TCAGCAGAAA CACAATAAAC GCTTTCTCGA AGGAGCAACT GGATTCCTTT 
CACAGGCTGC AGACCCTGGA GGCCGGTGGC AACAACTTCC TTTGCTCCTG TGAATTCCTG 
TCTTTCACTC AGGAGCAGCA GGCCCTGGCC GGGCTCCTGG TCGGCTGGCC AGAGGACTAC 
CTGTGCCACT CCCCCTCCTA CGTGCGGGGC CAGCGGGTTG GGACCGCCCG GCTCCCGGCT 
TCTGAGTGCC ACCGGACAGC TCTGGTGGCC GCCGTGTGCT GTGTCTTGCT CCTGCTGGTC 
CTGCTCACGG CGGGGGCGTG CCACCATTTC CACGGGCTGT GGTACCTGAG AATGCTGTGG 
GCCTGGCTCC AGGCCAAAAG GAAGCCCAGG AAAGCCCCCT CCAGGGACGT CTGTTATGAC 
GCCTTTGTGT CTTACAGTGA GCATGATTCC TACTGGGTGG AGAACCTTCT GGTCCAGAAG 
CTGGAGCACT TCAATCCCCC GTTCAAGTTG TGCCTTCACA AGCGGGACTT TATTCCCGGC 
AAGTGGATTA TTGACAATAT CATTGACTCC ATCGAGAAGA GCCACAAAAC CATCTTTGTG 
CTTTCTGAAA ACTTCGTGAA GAGCGAGTGG TGCAAGTACG AGCTGGACTT CTCCCATTTT 
CGCCTCTTTG ATGAGAACAA CGATGCTGCC ATCCTCATTC TTCTGGAGCC CATTGAGAAA 
AAGGCCATCC CCCAGCGATT CTGTAAGCTG CGGAAGATAA TGAACACCAA GACGTACCTG 
GAGTGGCCCA CCGATGATGC TCAGCAGGAA GGGTTTTGGT TAAATTTGAG AACAGCAATA 
AAATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022283364.1
CDS	50..2458
Translation	MTGNDREGADARLDHWTMSRVLWTLWVLGAVTNLSKEEAPDQSSSLSCDPTGVCDGRSRSLNSMPSGLTAAVRSLDLSNNEITYIGNSDLRDCVNLKALRLESNGINTIEEESFFSLWSLEHLDLSYNLLSNLSSSWFRPLSSLKFLNLLGNPYKSLGETPLFSQLTNLRILKVGNIYSFTEIQDKDFAGLTFLEELEIDASNLQRYEPKSLKSIQNISYLALRMKQPVLLVEIFVDLSSSLKHLELRDTHLDTFHFSEASINETHTLVKKWTFRNVKVTDRSFTEVVRLLNYVSGVLEVEFEDCTLYGLGDFDIPDVDKIKNIGQIETLTVRRLHIPHFYSFYDMSSIYSLTEDVKRITVESSKVFLVPCSLSQHLKSLEYLDLSDNLMVEEYLRNSACQHAWPLLQTLILRQNRLKSLEKTGETLLTLKNLVNLDISKNNYLSMPETCQWPEKLKCLNLSDTRMQSITRCIPQTLEILDVSNNNLESFSLILPQLKELSISRNKLKTLPDASFLPTLQIMRISRNTINAFSKEQLDSFHRLQTLEAGGNNFLCSCEFLSFTQEQQALAGLLVGWPEDYLCHSPSYVRGQRVGTARLPASECHRTALVAAVCCVLLLLVLLTAGACHHFHGLWYLRMLWAWLQAKRKPRKAPSRDVCYDAFVSYSEHDSYWVENLLVQKLEHFNPPFKLCLHKRDFIPGKWIIDNIIDSIEKSHKTIFVLSENFVKSEWCKYELDFSHFRLFDENNDAAILILLEPIEKKAIPQRFCKLRKIMNTKTYLEWPTDDAQQEGFWLNLRTAIKS

Target ORF information:

RefSeq Version	XM_022427656.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris toll like receptor 2 (TLR2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022427656.1

ORF Insert Sequence:

ATGACGGGGA ACGACCGGGA AGGAGCCGAC GCAAGGTTGG ATCACTGGAC AATGTCACGT 
GTTTTGTGGA CATTGTGGGT TTTGGGGGCT GTAACCAACC TCTCCAAGGA AGAGGCCCCT 
GACCAGTCTT CTTCTCTGTC CTGTGACCCC ACTGGTGTCT GCGATGGCCG CTCCAGATCT 
TTGAACTCCA TGCCCTCAGG GCTCACAGCA GCTGTGAGAA GCCTTGACCT CTCCAACAAT 
GAGATCACCT ACATTGGCAA CAGTGACCTT CGGGATTGTG TGAACCTCAA GGCTCTGAGG 
CTGGAGTCTA ATGGAATTAA CACAATAGAG GAAGAATCTT TTTTTTCCCT GTGGAGTCTT 
GAACATTTGG ACTTATCTTA TAACCTCTTA TCTAACTTAT CATCCTCCTG GTTCAGGCCC 
CTTTCTTCAT TGAAGTTCTT AAACCTACTG GGAAATCCTT ACAAATCACT TGGGGAAACA 
CCTCTTTTTT CTCAGCTCAC AAATCTAAGA ATTCTGAAAG TAGGAAATAT CTACAGCTTC 
ACTGAGATTC AGGATAAGGA TTTTGCTGGG CTAACCTTTC TTGAGGAACT GGAGATCGAT 
GCTTCAAATC TCCAGAGGTA TGAGCCAAAG AGTTTGAAAT CGATTCAGAA CATCAGCTAT 
CTGGCCCTCC GTATGAAGCA GCCTGTTTTA CTGGTGGAGA TTTTTGTAGA TCTTTCCAGT 
TCCTTGAAAC ATTTAGAACT GAGAGATACT CATTTGGACA CTTTCCACTT TTCAGAGGCA 
TCCATCAATG AAACACATAC GTTGGTTAAA AAGTGGACAT TTAGAAATGT GAAAGTCACC 
GATAGAAGTT TTACTGAGGT TGTGAGACTG TTGAATTATG TTTCTGGAGT GTTAGAAGTA 
GAGTTTGAGG ACTGTACCCT TTATGGGCTC GGTGATTTTG ACATACCTGA TGTGGACAAA 
ATTAAAAATA TAGGTCAGAT AGAGACACTA ACAGTACGGA GGTTGCATAT TCCACACTTT 
TACTCATTTT ACGATATGAG TAGTATATAT TCACTTACAG AAGATGTTAA AAGAATCACA 
GTAGAAAGCA GTAAGGTCTT TCTGGTTCCT TGCTCACTTT CACAACATTT AAAATCCCTA 
GAATATTTGG ATCTCAGCGA CAATTTAATG GTTGAAGAAT ACTTGAGAAA CTCAGCCTGT 
CAGCATGCTT GGCCCCTCCT GCAAACCTTA ATTTTAAGGC AAAATCGTTT GAAATCCTTA 
GAGAAAACTG GAGAAACTTT GCTTACTTTG AAAAACCTGG TGAACCTTGA TATTAGTAAG 
AATAATTATC TTTCTATGCC TGAAACTTGT CAGTGGCCAG AAAAGCTGAA ATGTTTGAAC 
TTATCCGACA CAAGAATGCA AAGTATAACC CGTTGCATCC CTCAGACACT GGAAATTTTA 
GATGTTAGCA ATAATAATCT CGAGTCATTT TCCCTGATTT TGCCACAACT TAAAGAACTT 
TCTATTTCCA GAAATAAGTT GAAGACTCTA CCAGATGCCT CCTTCTTACC CACCTTACAA 
ATTATGAGAA TCAGCAGAAA CACAATAAAC GCTTTCTCGA AGGAGCAACT GGATTCCTTT 
CACAGGCTGC AGACCCTGGA GGCCGGTGGC AACAACTTCC TTTGCTCCTG TGAATTCCTG 
TCTTTCACTC AGGAGCAGCA GGCCCTGGCC GGGCTCCTGG TCGGCTGGCC AGAGGACTAC 
CTGTGCCACT CCCCCTCCTA CGTGCGGGGC CAGCGGGTTG GGACCGCCCG GCTCCCGGCT 
TCTGAGTGCC ACCGGACAGC TCTGGTGGCC GCCGTGTGCT GTGTCTTGCT CCTGCTGGTC 
CTGCTCACGG CGGGGGCGTG CCACCATTTC CACGGGCTGT GGTACCTGAG AATGCTGTGG 
GCCTGGCTCC AGGCCAAAAG GAAGCCCAGG AAAGCCCCCT CCAGGGACGT CTGTTATGAC 
GCCTTTGTGT CTTACAGTGA GCATGATTCC TACTGGGTGG AGAACCTTCT GGTCCAGAAG 
CTGGAGCACT TCAATCCCCC GTTCAAGTTG TGCCTTCACA AGCGGGACTT TATTCCCGGC 
AAGTGGATTA TTGACAATAT CATTGACTCC ATCGAGAAGA GCCACAAAAC CATCTTTGTG 
CTTTCTGAAA ACTTCGTGAA GAGCGAGTGG TGCAAGTACG AGCTGGACTT CTCCCATTTT 
CGCCTCTTTG ATGAGAACAA CGATGCTGCC ATCCTCATTC TTCTGGAGCC CATTGAGAAA 
AAGGCCATCC CCCAGCGATT CTGTAAGCTG CGGAAGATAA TGAACACCAA GACGTACCTG 
GAGTGGCCCA CCGATGATGC TCAGCAGGAA GGGTTTTGGT TAAATTTGAG AACAGCAATA 
AAATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histopathological findings and detection of Toll-like receptor 2 in cutaneous lesions of canine leishmaniosis.
Veterinary parasitology209(3-4)157-63(2015 Apr)
Esteve LO,Saz SV,Hosein S,Solano-Gallego L

Compartmentalized gene expression of toll-like receptors 2, 4 and 9 in the brain and peripheral lymphoid organs during canine visceral leishmaniasis.
Parasite immunology36(12)726-31(2014 Dec)
Melo GD,Silva JE,Grano FG,Homem CG,Machado GF

Assessment of Toll-like receptor 2, 4 and 9 SNP genotypes in canine sino-nasal aspergillosis.
BMC veterinary research10187(2014 Aug)
Mercier E,Peters IR,Farnir F,Lavou? R,Day M,Clercx C,Peeters D

Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
BMC immunology1422(2013 May)
Figueiredo MM,Amorim IF,Pinto AJ,Barbosa VS,Pinheiro Lde J,Deoti B,Faria AM,Tafuri WL

The localization of Toll-like receptor 2 (TLR2) in the endometrium and the cervix of dogs at different stages of the oestrous cycle and with pyometra.
Reproduction in domestic animals = Zuchthygiene47 Suppl 6351-5(2012 Dec)
Chotimanukul S,Sirivaidyapong S

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TLR2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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