SERPINF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SERPINF1
Entrez Gene ID	281386
Full Name	serpin family F member 1
Synonyms	PEDF
General protein information	Preferred Names serpin family F member 1 Names pigment epithelium-derived factor serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 serpin F1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174140.3	NP_776565.1	pigment epithelium-derived factor precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SERPINF1	NP_001071056.2
Gallus gallus (chicken)	SERPINF1	NP_001244218.1
Homo sapiens (human)	SERPINF1	NP_002606.3
Pan troglodytes (chimpanzee)	SERPINF1	XP_001155004.1
Mus musculus (house mouse)	Serpinf1	NP_035470.3
Bos taurus (cattle)	SERPINF1	NP_776565.1
Rattus norvegicus (Norway rat)	Serpinf1	NP_808788.1
Danio rerio (zebrafish)	serpinf1	NP_001004539.1
Xenopus tropicalis (tropical clawed frog)	serpinf1	NP_989086.1

Gene Ontology
Bibliography

Related articles in PubMed

Antiangiogenic effects and transcriptional regulation of pigment epithelium-derived factor in diabetic retinopathy.
Wang B, Atherton P, Patel R, Manning G, Donnelly R
Microvascular research80(1)31-6(2010 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Pigment epithelium-derived factor inhibits angiogenesis via regulated intracellular proteolysis of vascular endothelial growth factor receptor 1.
Cai J, Jiang WG, Grant MB, Boulton M
The Journal of biological chemistry281(6)3604-13(2006 Feb)

Pigment epithelium-derived factor (PEDF) promotes growth of pericytes through autocrine production of platelet-derived growth factor-B.
Yamagishi S, Nakamura K, Takenaka K, Matsui T, Jinnouchi Y, Imaizumi T
Microvascular research69(3)128-34(2005 May)

Up-regulation of vascular endothelial growth factor and down-regulation of pigment epithelium-derived factor messenger ribonucleic acid levels in leptin-exposed cultured retinal pericytes.
Yamagishi S, Inagaki Y, Amano S, Okamoto T, Takeuchi M
International journal of tissue reactions24(4)137-42(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PEDF inhibits key steps in the angiogenic response of retinal capillary endothelial cells, including cell proliferation and vascular tubule formation. Gene expression of PEDF is negatively regulated by glucose, insulin (via mTOR) and VEGF.
Title: Antiangiogenic effects and transcriptional regulation of pigment epithelium-derived factor in diabetic retinopathy.

PEDF and VEGFR-1 have roles in the negative regulation of angiogenesis
Title: Pigment epithelium-derived factor inhibits angiogenesis via regulated intracellular proteolysis of vascular endothelial growth factor receptor 1.

PEDF promotes growth of pericytes through autocrine production of PDGFB.
Title: Pigment epithelium-derived factor (PEDF) promotes growth of pericytes through autocrine production of platelet-derived growth factor-B.

Results suggest that leptin might elicit angiogenesis through vascular endothelial growth factor induction as well as pigment epithelium-derived factor suppression in pericytes.
Title: Up-regulation of vascular endothelial growth factor and down-regulation of pigment epithelium-derived factor messenger ribonucleic acid levels in leptin-exposed cultured retinal pericytes.

The following SERPINF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SERPINF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44694	NM_174140.3 Latest version!	Bos taurus serpin family F member 1 (SERPINF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SERPINF1 ( NM_174140.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776565.1 Bos taurus serpin family F member 1 (SERPINF1), mRNA.

Price & Availability↑

CloneID	OBa44694
Clone ID Related Accession (Same CDS sequence)	NM_174140.3
Accession Version	NM_174140.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	pigment epithelium-derived factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF017058.1. On Aug 30, 2012 this sequence version replaced NM_174140.2. ##Evidence-Data-START## Transcript exon combination :: AF017058.1, BC112656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGCCC TCGTGCTACT CCTCTGGACT GGAGCCCTGC TTGGGTTTGG CCGCTGCCAG 
AACGCCGGCC AGGAGGCGGG CTCTCTGACC CCTGAGAGCA CGGGGGCACC AGTGGAGGAA 
GAGGATCCCT TCTTCAAGGT CCCTGTGAAC AAGCTGGCGG CAGCGGTCTC CAACTTCGGC 
TACGACCTGT ACCGCGTGAG ATCCGGTGAG AGCCCCACCG CCAATGTGCT GCTGTCTCCG 
CTCAGCGTGG CCACGGCGCT CTCTGCCCTG TCGCTGGGTG CGGAACAGCG GACAGAATCC 
AACATTCACC GGGCTCTGTA CTACGACCTG ATCAGTAACC CAGACATCCA CGGCACCTAC 
AAGGACCTCC TTGCCTCCGT CACCGCCCCC CAGAAGAACC TTAAGAGTGC TTCCCGGATT 
ATCTTTGAGA GGAAGCTGCG GATAAAAGCC AGCTTCATCC CACCCCTGGA GAAGTCATAT 
GGGACCAGGC CCAGAATCCT GACCGGCAAC TCTCGAGTAG ACCTTCAGGA GATTAACAAC 
TGGGTGCAGG CCCAGATGAA AGGGAAAGTC GCTAGGTCCA CGAGGGAGAT GCCCAGTGAG 
ATCAGCATTT TCCTCCTGGG CGTGGCTTAC TTCAAGGGGC AGTGGGTAAC AAAGTTTGAC 
TCCAGAAAAA CTTCCCTGGA GGATTTCTAC TTGGATGAGG AGAGGACCGT GAAAGTCCCC 
ATGATGTCAG ACCCTCAGGC CGTTTTACGG TACGGCTTGG ATTCTGATCT CAACTGCAAG 
ATCGCCCAGC TGCCCTTGAC CGGGAGCACA AGTATCATCT TCTTCCTGCC TCAGAAAGTG 
ACCCAGAACT TGACCTTGAT AGAAGAGAGC CTCACCTCTG AGTTCATTCA TGACATAGAC 
CGAGAACTGA AGACTGTTCA GGCGGTCCTG ACCATTCCCA AGCTGAAGCT GAGTTATGAA 
GGCGAACTCA CGAAGTCCGT GCAGGAGCTG AAGCTGCAAT CCCTGTTTGA TGCACCAGAC 
TTTAGCAAGA TCACAGGCAA ACCTATCAAA CTTACTCAAG TGGAACATCG CGTCGGATTT 
GAGTGGAATG AGGATGGGGC GGGTACTAAC TCCAGCCCAG GGGTCCAGCC TGCCCGCCTC 
ACCTTCCCTC TGGACTATCA CCTTAACCAA CCTTTCATCT TTGTACTGAG GGACACAGAC 
ACAGGGGCCC TTCTCTTCAT AGGCAAAATT CTGGACCCCA GGGGCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776565.1
CDS	22..1272
Translation	MQALVLLLWTGALLGFGRCQNAGQEAGSLTPESTGAPVEEEDPFFKVPVNKLAAAVSNFGYDLYRVRSGESPTANVLLSPLSVATALSALSLGAEQRTESNIHRALYYDLISNPDIHGTYKDLLASVTAPQKNLKSASRIIFERKLRIKASFIPPLEKSYGTRPRILTGNSRVDLQEINNWVQAQMKGKVARSTREMPSEISIFLLGVAYFKGQWVTKFDSRKTSLEDFYLDEERTVKVPMMSDPQAVLRYGLDSDLNCKIAQLPLTGSTSIIFFLPQKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTIPKLKLSYEGELTKSVQELKLQSLFDAPDFSKITGKPIKLTQVEHRVGFEWNEDGAGTNSSPGVQPARLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGT

Target ORF information:

RefSeq Version	NM_174140.3
Organism	Bos taurus(cattle)
Definition	Bos taurus serpin family F member 1 (SERPINF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174140.3

ORF Insert Sequence:

ATGCAGGCCC TCGTGCTACT CCTCTGGACT GGAGCCCTGC TTGGGTTTGG CCGCTGCCAG 
AACGCCGGCC AGGAGGCGGG CTCTCTGACC CCTGAGAGCA CGGGGGCACC AGTGGAGGAA 
GAGGATCCCT TCTTCAAGGT CCCTGTGAAC AAGCTGGCGG CAGCGGTCTC CAACTTCGGC 
TACGACCTGT ACCGCGTGAG ATCCGGTGAG AGCCCCACCG CCAATGTGCT GCTGTCTCCG 
CTCAGCGTGG CCACGGCGCT CTCTGCCCTG TCGCTGGGTG CGGAACAGCG GACAGAATCC 
AACATTCACC GGGCTCTGTA CTACGACCTG ATCAGTAACC CAGACATCCA CGGCACCTAC 
AAGGACCTCC TTGCCTCCGT CACCGCCCCC CAGAAGAACC TTAAGAGTGC TTCCCGGATT 
ATCTTTGAGA GGAAGCTGCG GATAAAAGCC AGCTTCATCC CACCCCTGGA GAAGTCATAT 
GGGACCAGGC CCAGAATCCT GACCGGCAAC TCTCGAGTAG ACCTTCAGGA GATTAACAAC 
TGGGTGCAGG CCCAGATGAA AGGGAAAGTC GCTAGGTCCA CGAGGGAGAT GCCCAGTGAG 
ATCAGCATTT TCCTCCTGGG CGTGGCTTAC TTCAAGGGGC AGTGGGTAAC AAAGTTTGAC 
TCCAGAAAAA CTTCCCTGGA GGATTTCTAC TTGGATGAGG AGAGGACCGT GAAAGTCCCC 
ATGATGTCAG ACCCTCAGGC CGTTTTACGG TACGGCTTGG ATTCTGATCT CAACTGCAAG 
ATCGCCCAGC TGCCCTTGAC CGGGAGCACA AGTATCATCT TCTTCCTGCC TCAGAAAGTG 
ACCCAGAACT TGACCTTGAT AGAAGAGAGC CTCACCTCTG AGTTCATTCA TGACATAGAC 
CGAGAACTGA AGACTGTTCA GGCGGTCCTG ACCATTCCCA AGCTGAAGCT GAGTTATGAA 
GGCGAACTCA CGAAGTCCGT GCAGGAGCTG AAGCTGCAAT CCCTGTTTGA TGCACCAGAC 
TTTAGCAAGA TCACAGGCAA ACCTATCAAA CTTACTCAAG TGGAACATCG CGTCGGATTT 
GAGTGGAATG AGGATGGGGC GGGTACTAAC TCCAGCCCAG GGGTCCAGCC TGCCCGCCTC 
ACCTTCCCTC TGGACTATCA CCTTAACCAA CCTTTCATCT TTGTACTGAG GGACACAGAC 
ACAGGGGCCC TTCTCTTCAT AGGCAAAATT CTGGACCCCA GGGGCACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Antiangiogenic effects and transcriptional regulation of pigment epithelium-derived factor in diabetic retinopathy.
Microvascular research80(1)31-6(2010 Jul)
Wang B,Atherton P,Patel R,Manning G,Donnelly R

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Pigment epithelium-derived factor inhibits angiogenesis via regulated intracellular proteolysis of vascular endothelial growth factor receptor 1.
The Journal of biological chemistry281(6)3604-13(2006 Feb)
Cai J,Jiang WG,Grant MB,Boulton M

Pigment epithelium-derived factor (PEDF) promotes growth of pericytes through autocrine production of platelet-derived growth factor-B.
Microvascular research69(3)128-34(2005 May)
Yamagishi S,Nakamura K,Takenaka K,Matsui T,Jinnouchi Y,Imaizumi T

Up-regulation of vascular endothelial growth factor and down-regulation of pigment epithelium-derived factor messenger ribonucleic acid levels in leptin-exposed cultured retinal pericytes.
International journal of tissue reactions24(4)137-42(2002)
Yamagishi S,Inagaki Y,Amano S,Okamoto T,Takeuchi M

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SERPINF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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