GHRL cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GHRL
Entrez Gene ID	281192
Full Name	ghrelin and obestatin prepropeptide
Synonyms	Gh1-1,ghrelin
General protein information	Preferred Names ghrelin and obestatin prepropeptide Names appetite-regulating hormone ghrelin/obestatin preprohormone growth hormone secretagogue growth hormone-releasing peptide motilin-related peptide
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005223021.4	XP_005223078.2	appetite-regulating hormone isoform X1
XM_005223021.3	XP_005223078.2	appetite-regulating hormone isoform X1
NM_174067.2	NP_776492.1	appetite-regulating hormone precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ghrl	NP_067701.1
Canis lupus familiaris (dog)	GHRL	NP_001003052.1
Pan troglodytes (chimpanzee)	GHRL	XP_003339081.1
	GHRL	NP_001028075.1
Homo sapiens (human)	GHRL	NP_057446.1
Bos taurus (cattle)	GHRL	NP_776492.1
Mus musculus (house mouse)	Ghrl	NP_067463.2

Gene Ontology
Bibliography

Related articles in PubMed

Polymorphisms in the GHRL gene and their associations with traits of economic interest in beef cattle.
Braz CU, Camargo GM, Cardoso DF, Gil FM, Fonseca PD, Cyrillo JN, Mercadante ME, Oliveira HN, Tonhati H
Genetics and molecular research : GMR14(4)18188-97(2015 Dec)

Leptin and ghrelin levels in colostrum, milk and blood plasma of sows and pig neonates during the first week of lactation.
Woli?ski J, S?upecka M, Romanowicz K
Animal science journal = Nihon chikusan Gakkaiho85(2)143-9(2014 Feb)

Effects of ghrelin gene genotypes on the growth traits in Chinese cattle.
Zhang AL, Zhang L, Zhang LZ, Zhang CF, Lan XY, Zhang CL, Chen H
Molecular biology reports39(6)6981-6(2012 Jun)

Circulating ghrelin and leptin concentrations and growth hormone secretagogue receptor abundance in liver, muscle, and adipose tissue of beef cattle exhibiting differences in composition of gain.
Jennings JS, Wertz-Lutz AE, Pritchard RH, Weaver AD, Keisler DH, Bruns K
Journal of animal science89(12)3954-72(2011 Dec)

Short communication: change in plasma ghrelin in dairy cows following an intravenous glucose challenge.
Roche JR, Sheahan AJ, Chagas LM, Boston RC
Journal of dairy science91(3)1005-10(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GHRL is an important candidate gene that may be used to identify genetic variations that influence traits of economic importance in beef cattle
Title: Polymorphisms in the GHRL gene and their associations with traits of economic interest in beef cattle.

It is involved both in the short-term regulation of feeding and in the long-term regulation of energy metabolisms which may affect growth and development of the neonate.
Title: Leptin and ghrelin levels in colostrum, milk and blood plasma of sows and pig neonates during the first week of lactation.

Single nucleotide polymorphisms identified in the promoter region of the ghrelin gene could cause changes in the putative transcription factor binding sites.
Title: Effects of ghrelin gene genotypes on the growth traits in Chinese cattle.

The effects of dietary energy on the metabolism of ghrelin, leptin and growth hormone secretagogue receptor in blood and tissues of steers are reported.
Title: Circulating ghrelin and leptin concentrations and growth hormone secretagogue receptor abundance in liver, muscle, and adipose tissue of beef cattle exhibiting differences in composition of gain.

ghrelin declined after glucose infusion and before the insulin peak; post-nadir surge in ghrelin may be regulated by the decline in circulating concentrations of glucose and nonesterified fatty acids
Title: Short communication: change in plasma ghrelin in dairy cows following an intravenous glucose challenge.

The following GHRL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GHRL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41674	NM_174067.2 Latest version!	Bos taurus ghrelin and obestatin prepropeptide (GHRL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa31713	XM_005223021.4 Latest version!	Bos taurus ghrelin and obestatin prepropeptide (GHRL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OBa31713	XM_005223021.3	Bos taurus ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GHRL ( NM_174067.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776492.1 Bos taurus ghrelin and obestatin prepropeptide (GHRL), mRNA.

Price & Availability↑

CloneID	OBa41674
Clone ID Related Accession (Same CDS sequence)	NM_174067.2
Accession Version	NM_174067.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	appetite-regulating hormone precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF350329.1. On Jun 3, 2003 this sequence version replaced NM_174067.1. ##Evidence-Data-START## Transcript exon combination :: AF350329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145432, SAMN03145435 [ECO:0000348] ##Evidence-Data-END##

ATGCCCGCCC CGTGGACCAT CTGCAGCCTG CTGCTGCTCA GCGTGCTCTG CATGGACTTG 
GCCATGGCGG GCTCCAGCTT TCTGAGCCCC GAACATCAGA AACTGCAGAG AAAGGAAGCT 
AAGAAGCCAT CAGGCAGACT GAAGCCCCGG ACCCTGGAAG GCCAGTTTGA CCCGGAGGTG 
GGAAGTCAGG CGGAAGGTGC AGAGGACGAG CTGGAAATCC GGTTCAACGC CCCCTTTAAC 
ATTGGGATCA AGCTAGCAGG GGCTCAGTCC CTCCAGCATG GCCAGACGTT GGGGAAGTTT 
CTTCAGGACA TCCTTTGGGA AGAAGCTGAA GAAACCCTGG CTAACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776492.1
CDS	4..354
Translation	MPAPWTICSLLLLSVLCMDLAMAGSSFLSPEHQKLQRKEAKKPSGRLKPRTLEGQFDPEVGSQAEGAEDELEIRFNAPFNIGIKLAGAQSLQHGQTLGKFLQDILWEEAEETLANE

Target ORF information:

RefSeq Version	NM_174067.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ghrelin and obestatin prepropeptide (GHRL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174067.2

ORF Insert Sequence:

ATGCCCGCCC CGTGGACCAT CTGCAGCCTG CTGCTGCTCA GCGTGCTCTG CATGGACTTG 
GCCATGGCGG GCTCCAGCTT TCTGAGCCCC GAACATCAGA AACTGCAGAG AAAGGAAGCT 
AAGAAGCCAT CAGGCAGACT GAAGCCCCGG ACCCTGGAAG GCCAGTTTGA CCCGGAGGTG 
GGAAGTCAGG CGGAAGGTGC AGAGGACGAG CTGGAAATCC GGTTCAACGC CCCCTTTAAC 
ATTGGGATCA AGCTAGCAGG GGCTCAGTCC CTCCAGCATG GCCAGACGTT GGGGAAGTTT 
CTTCAGGACA TCCTTTGGGA AGAAGCTGAA GAAACCCTGG CTAACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_005223021.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223078.2 Bos taurus ghrelin and obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa31713
Clone ID Related Accession (Same CDS sequence)	XM_005223021.4 , XM_005223021.3
Accession Version	XM_005223021.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	appetite-regulating hormone isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223021.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCCC CGTGGACCAT CTGCAGCCTG CTGCTGCTCA GCGTGCTCTG CATGGACTTG 
GCCATGGCGG GCTCCAGCTT TCTGAGCCCC GAACATCAGA AACTGCAGAG AAAGGAAGCT 
AAGAAGCCAT CAGGCAGACT GAAGCCCCGG ACCCTGGAAG GCCAGTTTGA CCCGGAGGTG 
GGAAGTCAGG CGGAAGGTGC AGAGGACGAG CTGGAAATCC GGTTCAACGC CCCCTTTAAC 
ATTGGGATCA AGCTAGCAGG GGCTCAGTCC CTCCAGCATG GCCAGACGTT GGGGAAGTTT 
CTTCAGGACA TCCTTTGGGA AGAAGCTGAA GCACATCTCC TCCGCCCCCT GTGGAAGGGA 
AGCCAAACCG GAGGGCAACG GGAGGAGAGC AGACACAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223078.2
CDS	74..475
Translation	MPAPWTICSLLLLSVLCMDLAMAGSSFLSPEHQKLQRKEAKKPSGRLKPRTLEGQFDPEVGSQAEGAEDELEIRFNAPFNIGIKLAGAQSLQHGQTLGKFLQDILWEEAEAHLLRPLWKGSQTGGQREESRHS

Target ORF information:

RefSeq Version	XM_005223021.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ghrelin and obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223021.4

ORF Insert Sequence:

ATGCCCGCCC CGTGGACCAT CTGCAGCCTG CTGCTGCTCA GCGTGCTCTG CATGGACTTG 
GCCATGGCGG GCTCCAGCTT TCTGAGCCCC GAACATCAGA AACTGCAGAG AAAGGAAGCT 
AAGAAGCCAT CAGGCAGACT GAAGCCCCGG ACCCTGGAAG GCCAGTTTGA CCCGGAGGTG 
GGAAGTCAGG CGGAAGGTGC AGAGGACGAG CTGGAAATCC GGTTCAACGC CCCCTTTAAC 
ATTGGGATCA AGCTAGCAGG GGCTCAGTCC CTCCAGCATG GCCAGACGTT GGGGAAGTTT 
CTTCAGGACA TCCTTTGGGA AGAAGCTGAA GCACATCTCC TCCGCCCCCT GTGGAAGGGA 
AGCCAAACCG GAGGGCAACG GGAGGAGAGC AGACACAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_005223021.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223078.2 Bos taurus ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa31713
Clone ID Related Accession (Same CDS sequence)	XM_005223021.4 , XM_005223021.3
Accession Version	XM_005223021.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	appetite-regulating hormone isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104545.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223021.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCCC CGTGGACCAT CTGCAGCCTG CTGCTGCTCA GCGTGCTCTG CATGGACTTG 
GCCATGGCGG GCTCCAGCTT TCTGAGCCCC GAACATCAGA AACTGCAGAG AAAGGAAGCT 
AAGAAGCCAT CAGGCAGACT GAAGCCCCGG ACCCTGGAAG GCCAGTTTGA CCCGGAGGTG 
GGAAGTCAGG CGGAAGGTGC AGAGGACGAG CTGGAAATCC GGTTCAACGC CCCCTTTAAC 
ATTGGGATCA AGCTAGCAGG GGCTCAGTCC CTCCAGCATG GCCAGACGTT GGGGAAGTTT 
CTTCAGGACA TCCTTTGGGA AGAAGCTGAA GCACATCTCC TCCGCCCCCT GTGGAAGGGA 
AGCCAAACCG GAGGGCAACG GGAGGAGAGC AGACACAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223078.2
CDS	77..478
Translation	MPAPWTICSLLLLSVLCMDLAMAGSSFLSPEHQKLQRKEAKKPSGRLKPRTLEGQFDPEVGSQAEGAEDELEIRFNAPFNIGIKLAGAQSLQHGQTLGKFLQDILWEEAEAHLLRPLWKGSQTGGQREESRHS

Target ORF information:

RefSeq Version	XM_005223021.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223021.3

ORF Insert Sequence:

ATGCCCGCCC CGTGGACCAT CTGCAGCCTG CTGCTGCTCA GCGTGCTCTG CATGGACTTG 
GCCATGGCGG GCTCCAGCTT TCTGAGCCCC GAACATCAGA AACTGCAGAG AAAGGAAGCT 
AAGAAGCCAT CAGGCAGACT GAAGCCCCGG ACCCTGGAAG GCCAGTTTGA CCCGGAGGTG 
GGAAGTCAGG CGGAAGGTGC AGAGGACGAG CTGGAAATCC GGTTCAACGC CCCCTTTAAC 
ATTGGGATCA AGCTAGCAGG GGCTCAGTCC CTCCAGCATG GCCAGACGTT GGGGAAGTTT 
CTTCAGGACA TCCTTTGGGA AGAAGCTGAA GCACATCTCC TCCGCCCCCT GTGGAAGGGA 
AGCCAAACCG GAGGGCAACG GGAGGAGAGC AGACACAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polymorphisms in the GHRL gene and their associations with traits of economic interest in beef cattle.
Genetics and molecular research : GMR14(4)18188-97(2015 Dec)
Braz CU,Camargo GM,Cardoso DF,Gil FM,Fonseca PD,Cyrillo JN,Mercadante ME,Oliveira HN,Tonhati H

Leptin and ghrelin levels in colostrum, milk and blood plasma of sows and pig neonates during the first week of lactation.
Animal science journal = Nihon chikusan Gakkaiho85(2)143-9(2014 Feb)
Woli?ski J,S?upecka M,Romanowicz K

Effects of ghrelin gene genotypes on the growth traits in Chinese cattle.
Molecular biology reports39(6)6981-6(2012 Jun)
Zhang AL,Zhang L,Zhang LZ,Zhang CF,Lan XY,Zhang CL,Chen H

Circulating ghrelin and leptin concentrations and growth hormone secretagogue receptor abundance in liver, muscle, and adipose tissue of beef cattle exhibiting differences in composition of gain.
Journal of animal science89(12)3954-72(2011 Dec)
Jennings JS,Wertz-Lutz AE,Pritchard RH,Weaver AD,Keisler DH,Bruns K

Short communication: change in plasma ghrelin in dairy cows following an intravenous glucose challenge.
Journal of dairy science91(3)1005-10(2008 Mar)
Roche JR,Sheahan AJ,Chagas LM,Boston RC