GHRL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GHRL
Entrez Gene ID	51738
Full Name	ghrelin and obestatin prepropeptide
Synonyms	MTLRP
General protein information	Preferred Names ghrelin and obestatin prepropeptide Names appetite-regulating hormone In2c-preproghrelin ghrelin, growth hormone secretagogue receptor ligand ghrelin/obestatin preprohormone ghrelin/obestatin prepropeptide growth hormone-releasing peptide motilin-related peptide prepro-appetite regulatory hormone preproghrelin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p25.3
Summary	This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014].
Disorder MIM:	605353
Disorder Html:	{Obesity, susceptibility to}, 601665 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134941.2	NP_001128413.1	appetite-regulating hormone isoform 2 preproprotein
NM_001302824.1	NP_001289753.1	appetite-regulating hormone isoform 1 preproprotein
NM_001134941.3	NP_001128413.1	appetite-regulating hormone isoform 2 preproprotein
XM_017006612.2	XP_016862101.1	appetite-regulating hormone isoform X1
XM_017006613.2	XP_016862102.1	appetite-regulating hormone isoform X2
XM_017006612.1	XP_016862101.1	appetite-regulating hormone isoform X1
NM_016362.4	NP_057446.1	appetite-regulating hormone isoform 1 preproprotein
NM_001302822.1	NP_001289751.1	appetite-regulating hormone isoform 1 preproprotein
NM_001134946.1	NP_001128418.1	appetite-regulating hormone isoform 5
NM_001134946.2	NP_001128418.1	appetite-regulating hormone isoform 5
XM_024453594.1	XP_024309362.1	appetite-regulating hormone isoform X1
NM_001134944.1	NP_001128416.1	appetite-regulating hormone isoform 3
NM_001302825.1	NP_001289754.1	appetite-regulating hormone isoform 1 preproprotein
NM_001302823.2	NP_001289752.1	appetite-regulating hormone isoform 2 preproprotein
NM_001302824.2	NP_001289753.1	appetite-regulating hormone isoform 1 preproprotein
NM_001302821.2	NP_001289750.1	appetite-regulating hormone isoform 1 preproprotein
NM_001134944.2	NP_001128416.1	appetite-regulating hormone isoform 3
XM_017006613.1	XP_016862102.1	appetite-regulating hormone isoform X2
NM_001302821.1	NP_001289750.1	appetite-regulating hormone isoform 1 preproprotein
NM_001134945.1	NP_001128417.1	appetite-regulating hormone isoform 4
NM_001302823.1	NP_001289752.1	appetite-regulating hormone isoform 2 preproprotein
NM_001302822.2	NP_001289751.1	appetite-regulating hormone isoform 1 preproprotein
NM_001302825.2	NP_001289754.1	appetite-regulating hormone isoform 1 preproprotein
NM_001134945.2	NP_001128417.1	appetite-regulating hormone isoform 4
NM_016362.5	NP_057446.1	appetite-regulating hormone isoform 1 preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ghrl	NP_067701.1
Canis lupus familiaris (dog)	GHRL	NP_001003052.1
Pan troglodytes (chimpanzee)	GHRL	XP_003339081.1
	GHRL	NP_001028075.1
Homo sapiens (human)	GHRL	NP_057446.1
Bos taurus (cattle)	GHRL	NP_776492.1
Mus musculus (house mouse)	Ghrl	NP_067463.2

Gene Ontology
Bibliography

Related articles in PubMed

Obestatin: its physicochemical characteristics and physiological functions.
Tang SQ, Jiang QY, Zhang YL, Zhu XT, Shu G, Gao P, Feng DY, Wang XQ, Dong XY
Peptides29(4)639-45(2008 Apr)

Revised genomic structure of the human ghrelin gene and identification of novel exons, alternative splice variants and natural antisense transcripts.
Seim I, Collet C, Herington AC, Chopin LK
BMC genomics8298(2007 Aug)

Ghrelin and a novel preproghrelin isoform are highly expressed in prostate cancer and ghrelin activates mitogen-activated protein kinase in prostate cancer.
Yeh AH, Jeffery PL, Duncan RP, Herington AC, Chopin LK
Clinical cancer research : an official journal of the American Association for Cancer Research11(23)8295-303(2005 Dec)

Genomic structure and characterization of the 5'-flanking region of the human ghrelin gene.
Kanamoto N, Akamizu T, Tagami T, Hataya Y, Moriyama K, Takaya K, Hosoda H, Kojima M, Kangawa K, Nakao K
Endocrinology145(9)4144-53(2004 Sep)

C-terminal pro-ghrelin peptides are present in the human circulation.
Pemberton C, Wimalasena P, Yandle T, Soule S, Richards M
Biochemical and biophysical research communications310(2)567-73(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Carrying a G to T substitution in rs696217 preproghrelin gene seems to mark a successful weight loss outcome after gastric bypass in morbidly obese patients
Title: Heterozygosity for the rs696217 SNP in the Preproghrelin Gene Predicts Weight Loss After Bariatric Surgery in Severely Obese Individuals.

studies indicate that pharmacological targeting of the endogenous ghrelin system is a valuable approach to treat metabolic complications.
Title: Therapeutic Potential of Targeting the Ghrelin Pathway.

Data suggest that subjects with anorexia nervosa display broad spectrum of physical activity (2479-26,047 steps/day) which shows a negative correlation with plasma kisspeptin levels and a positive association with plasma ghrelin levels.
Title: Plasma kisspeptin and ghrelin levels are independently correlated with physical activity in patients with anorexia nervosa.

Synovial fluid ghrelin levels demonstrated an independent and negative association with meniscus injury, cartilage damage, and clinical severity in patients with anterior cruciate ligament deficiency.
Title: Attenuated synovial fluid ghrelin levels are linked with cartilage damage, meniscus injury, and clinical symptoms in patients with knee anterior cruciate ligament deficiency.

Both leptin and ghrelin used separately at the concentrations corresponding to trimesters II-III of pregnancy increase the number of CD56(bright) NK cells in mononuclear cell suspension; their combination also enhances the L-selectin expression on the surface of these cells in the culture. These hormones do not affect the production of TGF-beta1, IL-17capital A, Cyrillic, or IFN-gamma by NK cells, and they inhibit the ...
Title: Roles of leptin and ghrelin in the regulation of the phenotype and cytokine production by NK cells from peripheral blood.

The following GHRL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GHRL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31622	NM_001302822.2 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31622	NM_016362.5 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31622	NM_001302824.2 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31622	NM_001302821.2 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31622	NM_001302825.2 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31622	XM_024453594.1 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31622	XM_017006612.2 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu32079	NM_001134941.3 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu32079	NM_001302823.2 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu32079	XM_017006613.2 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu07214	NM_001134946.2 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu19398	NM_001134944.2 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu31626	NM_001134945.2 Latest version!	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu32079	NM_001134941.2	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu31622	XM_017006612.1	Homo sapiens ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu07214	NM_001134946.1	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu31622	NM_001302824.1	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19398	NM_001134944.1	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu32079	XM_017006613.1	Homo sapiens ghrelin/obestatin prepropeptide (GHRL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu31622	NM_001302821.1	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu32079	NM_001302823.1	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu31622	NM_001302822.1	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31622	NM_001302825.1	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31626	NM_001134945.1	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu31622	NM_016362.4	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GHRL ( NM_001302824.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289753.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	NM_001302824.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072084.1, BC025791.1 and AY359053.1. On Nov 22, 2014 this sequence version replaced NR_024135.1. Transcript Variant: This variant (11) differs in the 5' UTR compared to variant 1. This isoform (11) contains the ligands ghrelin-28 and obestatin. Variants 1, 8, 9, 11 and 12 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF549572.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289753.1
CDS	333..686
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001302824.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302824.1

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_016362.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057446.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	NM_016362.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072083.1, EF549563.1, AY359053.1, EU072084.1 and BC025791.1. On or before Nov 22, 2014 this sequence version replaced NR_024132.1, NM_016362.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This isoform (1) contains the ligands ghrelin-28 and obestatin. Variants 1, 8, 9, 11 and 12 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU072083.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057446.1
CDS	872..1225
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_016362.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016362.4

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001302822.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289751.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	NM_001302822.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072084.1, BC025791.1 and AY359053.1. On Nov 22, 2014 this sequence version replaced NR_024134.1. Transcript Variant: This variant (9) differs in the 5' UTR compared to variant 1. This isoform (9) contains the ligands ghrelin-28 and obestatin. Variants 1, 8, 9, 11 and 12 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF549571.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289751.1
CDS	411..764
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001302822.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302822.1

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001302825.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289754.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	NM_001302825.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, BC025791.1 and AY359053.1. On Nov 22, 2014 this sequence version replaced NR_024136.1. Transcript Variant: This variant (12) differs in the 5' UTR compared to variant 1. This isoform (12) contains the ligands ghrelin-28 and obestatin. Variants 1, 8, 9, 11 and 12 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF549573.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2152474, SAMEA2153733 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289754.1
CDS	136..489
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001302825.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302825.1

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001302824.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289753.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	NM_001302824.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072084.1, BC025791.1, AY359053.1 and AC022384.5. On May 31, 2019 this sequence version replaced NM_001302824.1. Transcript Variant: This variant (11) differs in the 5' UTR compared to variant 1. This isoform (11) contains the ligands ghrelin-28 and obestatin. Variants 1, 8, 9, 11 and 12 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF549572.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289753.1
CDS	333..686
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001302824.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302824.2

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001302821.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289750.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	NM_001302821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072084.1, BC025791.1, AY359053.1 and AC022384.5. On May 31, 2019 this sequence version replaced NM_001302821.1. Transcript Variant: This variant (8) differs in the 5' UTR compared to variant 1. This isoform (8) contains the ligands ghrelin-28 and obestatin. Variants 1, 8, 9, 11 and 12 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU072087.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289750.1
CDS	623..976
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001302821.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302821.2

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001302821.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289750.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	NM_001302821.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072084.1, BC025791.1 and AY359053.1. On Nov 22, 2014 this sequence version replaced NR_024133.1. Transcript Variant: This variant (8) differs in the 5' UTR compared to variant 1. This isoform (8) contains the ligands ghrelin-28 and obestatin. Variants 1, 8, 9, 11 and 12 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU072087.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289750.1
CDS	623..976
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001302821.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302821.1

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001302822.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289751.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	NM_001302822.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072084.1, BC025791.1, AY359053.1 and AC022384.5. On May 31, 2019 this sequence version replaced NM_001302822.1. Transcript Variant: This variant (9) differs in the 5' UTR compared to variant 1. This isoform (9) contains the ligands ghrelin-28 and obestatin. Variants 1, 8, 9, 11 and 12 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF549571.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289751.1
CDS	411..764
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001302822.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302822.2

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001302825.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289754.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	NM_001302825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, BC025791.1, AY359053.1 and AC022384.5. On May 31, 2019 this sequence version replaced NM_001302825.1. Transcript Variant: This variant (12) differs in the 5' UTR compared to variant 1. This isoform (12) contains the ligands ghrelin-28 and obestatin. Variants 1, 8, 9, 11 and 12 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF549573.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2152474, SAMEA2153733 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289754.1
CDS	136..489
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001302825.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302825.2

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_016362.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057446.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	NM_016362.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072083.1, EF549563.1, AY359053.1, EU072084.1, BC025791.1 and AC022384.5. On May 17, 2019 this sequence version replaced NM_016362.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This isoform (1) contains the ligands ghrelin-28 and obestatin. Variants 1, 8, 9, 11 and 12 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU072083.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000335542.13/ ENSP00000335074.8 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057446.1
CDS	872..1225
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_016362.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016362.5

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_017006612.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862101.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	XM_017006612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006612.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862101.1
CDS	2185..2538
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	XM_017006612.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006612.2

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_017006612.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862101.1 Homo sapiens ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	XM_017006612.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862101.1
CDS	1407..1760
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	XM_017006612.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006612.1

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_024453594.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309362.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu31622
Clone ID Related Accession (Same CDS sequence)	NM_001302824.1 , XM_017006612.2 , XM_017006612.1 , NM_016362.4 , NM_001302822.1 , XM_024453594.1 , NM_001302825.1 , NM_001302824.2 , NM_001302821.2 , NM_001302821.1 , NM_001302822.2 , NM_001302825.2 , NM_016362.5
Accession Version	XM_024453594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309362.1
CDS	1936..2289
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	XM_024453594.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453594.1

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGCA GAGAAAGGAG 
TCGAAGAAGC CACCAGCCAA GCTGCAGCCC CGAGCTCTAG CAGGCTGGCT CCGCCCGGAA 
GATGGAGGTC AAGCAGAAGG GGCAGAGGAT GAACTGGAAG TCCGGTTCAA CGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGGTTCAG TACCAGCAGC ACAGCCAGGC CCTGGGGAAG 
TTTCTTCAGG ACATCCTCTG GGAAGAGGCC AAAGAGGCCC CAGCCGACAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001134941.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128413.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu32079
Clone ID Related Accession (Same CDS sequence)	NM_001134941.2 , NM_001134941.3 , XM_017006613.2 , NM_001302823.2 , XM_017006613.1 , NM_001302823.1
Accession Version	NM_001134941.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072084.1, EF549563.1, EU072085.1 and BC025791.1. On or before Nov 22, 2014 this sequence version replaced NR_024137.1, NM_001134941.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting isoform (2) contains the ligands ghrelin-27, which lacks a single amino acid compared to ghrelin-28 found in isoform 1, and obestatin. Variants 2 and 10 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU072084.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128413.1
CDS	872..1222
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001134941.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134941.2

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001134941.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128413.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu32079
Clone ID Related Accession (Same CDS sequence)	NM_001134941.2 , NM_001134941.3 , XM_017006613.2 , NM_001302823.2 , XM_017006613.1 , NM_001302823.1
Accession Version	NM_001134941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072084.1, EF549563.1, EU072085.1, BC025791.1 and AC022384.5. On Jun 1, 2019 this sequence version replaced NM_001134941.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting isoform (2) contains the ligands ghrelin-27, which lacks a single amino acid compared to ghrelin-28 found in isoform 1, and obestatin. Variants 2 and 10 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU072084.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128413.1
CDS	872..1222
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001134941.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134941.3

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001302823.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289752.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu32079
Clone ID Related Accession (Same CDS sequence)	NM_001134941.2 , NM_001134941.3 , XM_017006613.2 , NM_001302823.2 , XM_017006613.1 , NM_001302823.1
Accession Version	NM_001302823.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072084.1, BC025791.1 and AC022384.5. On May 31, 2019 this sequence version replaced NM_001302823.1. Transcript Variant: This variant (10) differs in the 5' UTR and uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting isoform (2) contains the ligands ghrelin-27, which lacks a single amino acid compared to ghrelin-28 found in isoform 1, and obestatin. Variants 2 and 10 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU072085.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289752.1
CDS	411..761
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001302823.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302823.2

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001302823.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289752.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu32079
Clone ID Related Accession (Same CDS sequence)	NM_001134941.2 , NM_001134941.3 , XM_017006613.2 , NM_001302823.2 , XM_017006613.1 , NM_001302823.1
Accession Version	NM_001302823.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072084.1 and BC025791.1. On Nov 22, 2014 this sequence version replaced NR_024138.1. Transcript Variant: This variant (10) differs in the 5' UTR and uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The resulting isoform (2) contains the ligands ghrelin-27, which lacks a single amino acid compared to ghrelin-28 found in isoform 1, and obestatin. Variants 2 and 10 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU072085.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289752.1
CDS	411..761
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001302823.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302823.1

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_017006613.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862102.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu32079
Clone ID Related Accession (Same CDS sequence)	NM_001134941.2 , NM_001134941.3 , XM_017006613.2 , NM_001302823.2 , XM_017006613.1 , NM_001302823.1
Accession Version	XM_017006613.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006613.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862102.1
CDS	2185..2535
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	XM_017006613.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006613.2

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_017006613.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862102.1 Homo sapiens ghrelin/obestatin prepropeptide (GHRL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu32079
Clone ID Related Accession (Same CDS sequence)	NM_001134941.2 , NM_001134941.3 , XM_017006613.2 , NM_001302823.2 , XM_017006613.1 , NM_001302823.1
Accession Version	XM_017006613.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862102.1
CDS	1416..1766
Translation	MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	XM_017006613.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin/obestatin prepropeptide (GHRL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006613.1

ORF Insert Sequence:

ATGCCCTCCC CAGGGACCGT CTGCAGCCTC CTGCTCCTCG GCATGCTCTG GCTGGACTTG 
GCCATGGCAG GCTCCAGCTT CCTGAGCCCT GAACACCAGA GAGTCCAGAG AAAGGAGTCG 
AAGAAGCCAC CAGCCAAGCT GCAGCCCCGA GCTCTAGCAG GCTGGCTCCG CCCGGAAGAT 
GGAGGTCAAG CAGAAGGGGC AGAGGATGAA CTGGAAGTCC GGTTCAACGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GGTTCAGTAC CAGCAGCACA GCCAGGCCCT GGGGAAGTTT 
CTTCAGGACA TCCTCTGGGA AGAGGCCAAA GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001134946.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128418.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu07214
Clone ID Related Accession (Same CDS sequence)	NM_001134946.1 , NM_001134946.2
Accession Version	NM_001134946.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EF549575.1 and BC025791.1. Transcript Variant: This variant (5) lacks three alternate in-frame exons in the 5' coding region but maintains the reading frame, compared to variant 1. The resulting isoform (5) is shorter compared to isoform 1 and lacks the N-terminal region that encodes the signal peptide and ghrelin-28 ligand found in isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF549575.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2152474, SAMEA2154405 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGTTCAACGC CCCCTTTGAT GTTGGAATCA AGCTGTCAGG GGTTCAGTAC 
CAGCAGCACA GCCAGGCCCT GGGGAAGTTT CTTCAGGACA TCCTCTGGGA AGAGGCCAAA 
GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128418.1
CDS	35..235
Translation	MFTCWWSYLRSTLAAVPGEASRVQFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001134946.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134946.1

ORF Insert Sequence:

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGTTCAACGC CCCCTTTGAT GTTGGAATCA AGCTGTCAGG GGTTCAGTAC 
CAGCAGCACA GCCAGGCCCT GGGGAAGTTT CTTCAGGACA TCCTCTGGGA AGAGGCCAAA 
GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001134946.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128418.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu07214
Clone ID Related Accession (Same CDS sequence)	NM_001134946.1 , NM_001134946.2
Accession Version	NM_001134946.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EF549575.1, BC025791.1 and AC022384.5. On May 31, 2019 this sequence version replaced NM_001134946.1. Transcript Variant: This variant (5) lacks three alternate in-frame exons in the 5' coding region but maintains the reading frame, compared to variant 1. The resulting isoform (5) is shorter compared to isoform 1 and lacks the N-terminal region that encodes the signal peptide and ghrelin-28 ligand found in isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF549575.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2152474, SAMEA2154405 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGTTCAACGC CCCCTTTGAT GTTGGAATCA AGCTGTCAGG GGTTCAGTAC 
CAGCAGCACA GCCAGGCCCT GGGGAAGTTT CTTCAGGACA TCCTCTGGGA AGAGGCCAAA 
GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128418.1
CDS	35..235
Translation	MFTCWWSYLRSTLAAVPGEASRVQFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001134946.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134946.2

ORF Insert Sequence:

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGTTCAACGC CCCCTTTGAT GTTGGAATCA AGCTGTCAGG GGTTCAGTAC 
CAGCAGCACA GCCAGGCCCT GGGGAAGTTT CTTCAGGACA TCCTCTGGGA AGAGGCCAAA 
GAGGCCCCAG CCGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001134944.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128416.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu19398
Clone ID Related Accession (Same CDS sequence)	NM_001134944.1 , NM_001134944.2
Accession Version	NM_001134944.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EF549574.1 and BC025791.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 5' coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) is shorter compared to isoform 1 and lacks the N-terminal region that encodes the signal peptide and ghrelin-28 ligand found in isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF549574.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2152474, SAMEA2154405 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGCAGAGAAA GGAGTCGAAG AAGCCACCAG CCAAGCTGCA GCCCCGAGCT 
CTAGCAGGCT GGCTCCGCCC GGAAGATGGA GGTCAAGCAG AAGGGGCAGA GGATGAACTG 
GAAGTCCGGT TCAACGCCCC CTTTGATGTT GGAATCAAGC TGTCAGGGGT TCAGTACCAG 
CAGCACAGCC AGGCCCTGGG GAAGTTTCTT CAGGACATCC TCTGGGAAGA GGCCAAAGAG 
GCCCCAGCCG ACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128416.1
CDS	35..352
Translation	MFTCWWSYLRSTLAAVPGEASRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001134944.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134944.1

ORF Insert Sequence:

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGCAGAGAAA GGAGTCGAAG AAGCCACCAG CCAAGCTGCA GCCCCGAGCT 
CTAGCAGGCT GGCTCCGCCC GGAAGATGGA GGTCAAGCAG AAGGGGCAGA GGATGAACTG 
GAAGTCCGGT TCAACGCCCC CTTTGATGTT GGAATCAAGC TGTCAGGGGT TCAGTACCAG 
CAGCACAGCC AGGCCCTGGG GAAGTTTCTT CAGGACATCC TCTGGGAAGA GGCCAAAGAG 
GCCCCAGCCG ACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001134944.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128416.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu19398
Clone ID Related Accession (Same CDS sequence)	NM_001134944.1 , NM_001134944.2
Accession Version	NM_001134944.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EF549574.1, BC025791.1 and AC022384.5. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001134944.1. Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 5' coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) is shorter compared to isoform 1 and lacks the N-terminal region that encodes the signal peptide and ghrelin-28 ligand found in isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF549574.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2152474, SAMEA2154405 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGCAGAGAAA GGAGTCGAAG AAGCCACCAG CCAAGCTGCA GCCCCGAGCT 
CTAGCAGGCT GGCTCCGCCC GGAAGATGGA GGTCAAGCAG AAGGGGCAGA GGATGAACTG 
GAAGTCCGGT TCAACGCCCC CTTTGATGTT GGAATCAAGC TGTCAGGGGT TCAGTACCAG 
CAGCACAGCC AGGCCCTGGG GAAGTTTCTT CAGGACATCC TCTGGGAAGA GGCCAAAGAG 
GCCCCAGCCG ACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128416.1
CDS	35..352
Translation	MFTCWWSYLRSTLAAVPGEASRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001134944.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134944.2

ORF Insert Sequence:

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGCAGAGAAA GGAGTCGAAG AAGCCACCAG CCAAGCTGCA GCCCCGAGCT 
CTAGCAGGCT GGCTCCGCCC GGAAGATGGA GGTCAAGCAG AAGGGGCAGA GGATGAACTG 
GAAGTCCGGT TCAACGCCCC CTTTGATGTT GGAATCAAGC TGTCAGGGGT TCAGTACCAG 
CAGCACAGCC AGGCCCTGGG GAAGTTTCTT CAGGACATCC TCTGGGAAGA GGCCAAAGAG 
GCCCCAGCCG ACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001134945.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128417.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu31626
Clone ID Related Accession (Same CDS sequence)	NM_001134945.1 , NM_001134945.2
Accession Version	NM_001134945.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-16
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072086.1 and BC025791.1. Transcript Variant: This variant (4) lacks two alternate in-frame exons and uses an alternate in-frame splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The resulting isoform (4) is shorter compared to isoform 1 and lacks the N-terminal region that encodes the signal peptide and ghrelin-28 ligand found in isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU072086.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2152474, SAMEA2154405 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGAGAAAGGA GTCGAAGAAG CCACCAGCCA AGCTGCAGCC CCGAGCTCTA 
GCAGGCTGGC TCCGCCCGGA AGATGGAGGT CAAGCAGAAG GGGCAGAGGA TGAACTGGAA 
GTCCGGTTCA ACGCCCCCTT TGATGTTGGA ATCAAGCTGT CAGGGGTTCA GTACCAGCAG 
CACAGCCAGG CCCTGGGGAA GTTTCTTCAG GACATCCTCT GGGAAGAGGC CAAAGAGGCC 
CCAGCCGACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128417.1
CDS	35..349
Translation	MFTCWWSYLRSTLAAVPGEASRVQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001134945.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134945.1

ORF Insert Sequence:

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGAGAAAGGA GTCGAAGAAG CCACCAGCCA AGCTGCAGCC CCGAGCTCTA 
GCAGGCTGGC TCCGCCCGGA AGATGGAGGT CAAGCAGAAG GGGCAGAGGA TGAACTGGAA 
GTCCGGTTCA ACGCCCCCTT TGATGTTGGA ATCAAGCTGT CAGGGGTTCA GTACCAGCAG 
CACAGCCAGG CCCTGGGGAA GTTTCTTCAG GACATCCTCT GGGAAGAGGC CAAAGAGGCC 
CCAGCCGACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( NM_001134945.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128417.1 Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 4, mRNA.

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CloneID	OHu31626
Clone ID Related Accession (Same CDS sequence)	NM_001134945.1 , NM_001134945.2
Accession Version	NM_001134945.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	appetite-regulating hormone isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF549561.1, EU072086.1, BC025791.1 and AC022384.5. On May 31, 2019 this sequence version replaced NM_001134945.1. Transcript Variant: This variant (4) lacks two alternate in-frame exons and uses an alternate in-frame splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The resulting isoform (4) is shorter compared to isoform 1 and lacks the N-terminal region that encodes the signal peptide and ghrelin-28 ligand found in isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU072086.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2152474, SAMEA2154405 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGAGAAAGGA GTCGAAGAAG CCACCAGCCA AGCTGCAGCC CCGAGCTCTA 
GCAGGCTGGC TCCGCCCGGA AGATGGAGGT CAAGCAGAAG GGGCAGAGGA TGAACTGGAA 
GTCCGGTTCA ACGCCCCCTT TGATGTTGGA ATCAAGCTGT CAGGGGTTCA GTACCAGCAG 
CACAGCCAGG CCCTGGGGAA GTTTCTTCAG GACATCCTCT GGGAAGAGGC CAAAGAGGCC 
CCAGCCGACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128417.1
CDS	35..349
Translation	MFTCWWSYLRSTLAAVPGEASRVQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK

Target ORF information:

RefSeq Version	NM_001134945.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ghrelin and obestatin prepropeptide (GHRL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134945.2

ORF Insert Sequence:

ATGTTTACTT GCTGGTGGTC TTATCTAAGA TCAACATTGG CAGCTGTGCC CGGAGAGGCC 
TCCAGGGTCC AGAGAAAGGA GTCGAAGAAG CCACCAGCCA AGCTGCAGCC CCGAGCTCTA 
GCAGGCTGGC TCCGCCCGGA AGATGGAGGT CAAGCAGAAG GGGCAGAGGA TGAACTGGAA 
GTCCGGTTCA ACGCCCCCTT TGATGTTGGA ATCAAGCTGT CAGGGGTTCA GTACCAGCAG 
CACAGCCAGG CCCTGGGGAA GTTTCTTCAG GACATCCTCT GGGAAGAGGC CAAAGAGGCC 
CCAGCCGACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Obestatin: its physicochemical characteristics and physiological functions.
Peptides29(4)639-45(2008 Apr)
Tang SQ,Jiang QY,Zhang YL,Zhu XT,Shu G,Gao P,Feng DY,Wang XQ,Dong XY

Revised genomic structure of the human ghrelin gene and identification of novel exons, alternative splice variants and natural antisense transcripts.
BMC genomics8298(2007 Aug)
Seim I,Collet C,Herington AC,Chopin LK

Ghrelin and a novel preproghrelin isoform are highly expressed in prostate cancer and ghrelin activates mitogen-activated protein kinase in prostate cancer.
Clinical cancer research : an official journal of the American Association for Cancer Research11(23)8295-303(2005 Dec)
Yeh AH,Jeffery PL,Duncan RP,Herington AC,Chopin LK

Genomic structure and characterization of the 5'-flanking region of the human ghrelin gene.
Endocrinology145(9)4144-53(2004 Sep)
Kanamoto N,Akamizu T,Tagami T,Hataya Y,Moriyama K,Takaya K,Hosoda H,Kojima M,Kangawa K,Nakao K

C-terminal pro-ghrelin peptides are present in the human circulation.
Biochemical and biophysical research communications310(2)567-73(2003 Oct)
Pemberton C,Wimalasena P,Yandle T,Soule S,Richards M

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GHRL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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