GHRL cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GHRL
Entrez Gene ID	403587
Full Name	ghrelin and obestatin prepropeptide
Synonyms	MTLRP,MTLRP-AP
General protein information	Preferred Names ghrelin and obestatin prepropeptide Names appetite-regulating hormone ghrelin, growth hormone secretagogue receptor ligand ghrelin/obestatin preprohormone growth hormone secretagogue growth hormone-releasing peptide motilin related peptide
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005632010.2	XP_005632067.1	appetite-regulating hormone isoform X3
XM_014121531.1	XP_013977006.1	appetite-regulating hormone isoform X2
NM_001003052.2	NP_001003052.1	appetite-regulating hormone preproprotein
XM_014121530.1	XP_013977005.1	appetite-regulating hormone isoform X1
XM_005632010.3	XP_005632067.1	appetite-regulating hormone isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ghrl	NP_067701.1
Canis lupus familiaris (dog)	GHRL	NP_001003052.1
Pan troglodytes (chimpanzee)	GHRL	XP_003339081.1
	GHRL	NP_001028075.1
Homo sapiens (human)	GHRL	NP_057446.1
Bos taurus (cattle)	GHRL	NP_776492.1
Mus musculus (house mouse)	Ghrl	NP_067463.2

Gene Ontology
Bibliography

Related articles in PubMed

The gut hormone ghrelin partially reverses energy substrate metabolic alterations in the failing heart.
Mitacchione G, Powers JC, Grifoni G, Woitek F, Lam A, Ly L, Settanni F, Makarewich CA, McCormick R, Trovato L, Houser SR, Granata R, Recchia FA
Circulation. Heart failure7(4)643-51(2014 Jul)

A role of ghrelin in canine mammary carcinoma cells proliferation, apoptosis and migration.
Majchrzak K, Paw?owski KM, Orzechowska EJ, Dolka I, Mucha J, Motyl T, Kr?l M
BMC veterinary research8170(2012 Sep)

Coronary vasoconstrictor effect of ghrelin is not mediated by growth hormone secretagogue receptor 1a type in dogs.
Sax B, Nadasy GL, Turi K, Hirschberg K, Furjesz D, Nagy A, Merkely B, Szabo G, Monos E, Kekesi V
Peptides32(2)362-7(2011 Feb)

Identification of cDNA encoding motilin related peptide/ghrelin precursor from dog fundus.
Tomasetto C, Wendling C, Rio MC, Poitras P
Peptides22(12)2055-9(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ghrelin is an endogenously produced peptide; hence, it might prove a biocompatible candidate for the correction of cardiac metabolic alterations.
Title: The gut hormone ghrelin partially reverses energy substrate metabolic alterations in the failing heart.

These experiments conducted in vitro confirmed that ghrelin promotes cancer development and metastasis.
Title: A role of ghrelin in canine mammary carcinoma cells proliferation, apoptosis and migration.

Ghrelin induces tone-dependent constriction of canine coronary arterioles which is mediated by a receptor other than GHS-R1a.
Title: Coronary vasoconstrictor effect of ghrelin is not mediated by growth hormone secretagogue receptor 1a type in dogs.

The following GHRL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GHRL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe34314	NM_001003052.2 Latest version!	Canis lupus familiaris ghrelin and obestatin prepropeptide (GHRL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe21106	XM_005632010.3 Latest version!	Canis lupus familiaris ghrelin and obestatin prepropeptide (GHRL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe41442	XM_014121530.1 Latest version!	Canis lupus familiaris ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OCe41443	XM_014121531.1 Latest version!	Canis lupus familiaris ghrelin/obestatin prepropeptide (GHRL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
View Old Accession Versions >>
OCe21106	XM_005632010.2	Canis lupus familiaris ghrelin/obestatin prepropeptide (GHRL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GHRL ( NM_001003052.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003052.1 Canis lupus familiaris ghrelin and obestatin prepropeptide (GHRL), mRNA.

Price & Availability↑

CloneID	OCe34314
Clone ID Related Accession (Same CDS sequence)	NM_001003052.2
Accession Version	NM_001003052.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Canis lupus familiaris(dog)
Product	appetite-regulating hormone preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB060700.1. On Jun 21, 2011 this sequence version replaced NM_001003052.1. ##Evidence-Data-START## Transcript exon combination :: AB060700.1, AJ298295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN07419798, SAMN07419808 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTCCC TGGGGACCAT GTGCAGCCTG CTGCTCTTCA GTGTGCTCTG GGTGGACCTG 
GCCATGGCGG GCTCCAGCTT CCTAAGTCCC GAACACCAGA AACTACAGCA GAGAAAGGAG 
TCCAAGAAGC CGCCGGCCAA ACTGCAGCCC CGAGCCCTAG AAGGCTCCCT TGGCCCAGAA 
GACACAAGTC AAGTGGAAGA GGCAGAGGAT GAGCTGGAAA TCCGGTTCAA TGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGCCTCAG TACCACCAGC ATGGCCAGGC ACTCGGGAAG 
TTTCTTCAAG AGGTTCTTTG GGAAGACACC AACGAGGCCC TGGCAGACGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003052.1
CDS	18..371
Translation	MPSLGTMCSLLLFSVLWVDLAMAGSSFLSPEHQKLQQRKESKKPPAKLQPRALEGSLGPEDTSQVEEAEDELEIRFNAPFDVGIKLSGPQYHQHGQALGKFLQEVLWEDTNEALADE

Target ORF information:

RefSeq Version	NM_001003052.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ghrelin and obestatin prepropeptide (GHRL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003052.2

ORF Insert Sequence:

ATGCCCTCCC TGGGGACCAT GTGCAGCCTG CTGCTCTTCA GTGTGCTCTG GGTGGACCTG 
GCCATGGCGG GCTCCAGCTT CCTAAGTCCC GAACACCAGA AACTACAGCA GAGAAAGGAG 
TCCAAGAAGC CGCCGGCCAA ACTGCAGCCC CGAGCCCTAG AAGGCTCCCT TGGCCCAGAA 
GACACAAGTC AAGTGGAAGA GGCAGAGGAT GAGCTGGAAA TCCGGTTCAA TGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGCCTCAG TACCACCAGC ATGGCCAGGC ACTCGGGAAG 
TTTCTTCAAG AGGTTCTTTG GGAAGACACC AACGAGGCCC TGGCAGACGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_005632010.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005632067.1 Canis lupus familiaris ghrelin/obestatin prepropeptide (GHRL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe21106
Clone ID Related Accession (Same CDS sequence)	XM_005632010.2 , XM_005632010.3
Accession Version	XM_005632010.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	appetite-regulating hormone isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726095.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005632010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC TGGGGACCAT GTGCAGCCTG CTGCTCTTCA GTGTGCTCTG GGTGGACCTG 
GCCATGGCGG GCTCCAGCTT CCTAAGTCCC GAACACCAGA AACTACAGAG AAAGGAGTCC 
AAGAAGCCGC CGGCCAAACT GCAGCCCCGA GCCCTAGAAG GCTCCCTTGG CCCAGAAGAC 
ACAAGTCAAG TGGAAGAGGC AGAGGATGAG CTGGAAATCC GGTTCAATGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GCCTCAGTAC CACCAGCATG GCCAGGCACT CGGGAAGTTT 
CTTCAAGAGG TTCTTTGGGA AGACACCAAC GAGGCCCTGG CAGACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005632067.1
CDS	144..494
Translation	MPSLGTMCSLLLFSVLWVDLAMAGSSFLSPEHQKLQRKESKKPPAKLQPRALEGSLGPEDTSQVEEAEDELEIRFNAPFDVGIKLSGPQYHQHGQALGKFLQEVLWEDTNEALADE

Target ORF information:

RefSeq Version	XM_005632010.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ghrelin/obestatin prepropeptide (GHRL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632010.2

ORF Insert Sequence:

ATGCCCTCCC TGGGGACCAT GTGCAGCCTG CTGCTCTTCA GTGTGCTCTG GGTGGACCTG 
GCCATGGCGG GCTCCAGCTT CCTAAGTCCC GAACACCAGA AACTACAGAG AAAGGAGTCC 
AAGAAGCCGC CGGCCAAACT GCAGCCCCGA GCCCTAGAAG GCTCCCTTGG CCCAGAAGAC 
ACAAGTCAAG TGGAAGAGGC AGAGGATGAG CTGGAAATCC GGTTCAATGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GCCTCAGTAC CACCAGCATG GCCAGGCACT CGGGAAGTTT 
CTTCAAGAGG TTCTTTGGGA AGACACCAAC GAGGCCCTGG CAGACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_005632010.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005632067.1 Canis lupus familiaris ghrelin and obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe21106
Clone ID Related Accession (Same CDS sequence)	XM_005632010.2 , XM_005632010.3
Accession Version	XM_005632010.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	appetite-regulating hormone isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006602.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005632010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC TGGGGACCAT GTGCAGCCTG CTGCTCTTCA GTGTGCTCTG GGTGGACCTG 
GCCATGGCGG GCTCCAGCTT CCTAAGTCCC GAACACCAGA AACTACAGAG AAAGGAGTCC 
AAGAAGCCGC CGGCCAAACT GCAGCCCCGA GCCCTAGAAG GCTCCCTTGG CCCAGAAGAC 
ACAAGTCAAG TGGAAGAGGC AGAGGATGAG CTGGAAATCC GGTTCAATGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GCCTCAGTAC CACCAGCATG GCCAGGCACT CGGGAAGTTT 
CTTCAAGAGG TTCTTTGGGA AGACACCAAC GAGGCCCTGG CAGACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005632067.1
CDS	54..404
Translation	MPSLGTMCSLLLFSVLWVDLAMAGSSFLSPEHQKLQRKESKKPPAKLQPRALEGSLGPEDTSQVEEAEDELEIRFNAPFDVGIKLSGPQYHQHGQALGKFLQEVLWEDTNEALADE

Target ORF information:

RefSeq Version	XM_005632010.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ghrelin and obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005632010.3

ORF Insert Sequence:

ATGCCCTCCC TGGGGACCAT GTGCAGCCTG CTGCTCTTCA GTGTGCTCTG GGTGGACCTG 
GCCATGGCGG GCTCCAGCTT CCTAAGTCCC GAACACCAGA AACTACAGAG AAAGGAGTCC 
AAGAAGCCGC CGGCCAAACT GCAGCCCCGA GCCCTAGAAG GCTCCCTTGG CCCAGAAGAC 
ACAAGTCAAG TGGAAGAGGC AGAGGATGAG CTGGAAATCC GGTTCAATGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GCCTCAGTAC CACCAGCATG GCCAGGCACT CGGGAAGTTT 
CTTCAAGAGG TTCTTTGGGA AGACACCAAC GAGGCCCTGG CAGACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_014121530.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977005.1 Canis lupus familiaris ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe41442
Clone ID Related Accession (Same CDS sequence)	XM_014121530.1
Accession Version	XM_014121530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	appetite-regulating hormone isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726095.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC TGGGGACCAT GTGCAGCCTG CTGCTCTTCA GTGTGCTCTG GGTGGACCTG 
GCCATGGCGG GCTCCAGCTT CCTAAGTCCC GAACACCAGA AACTACAGCA GAGAAAGGAG 
TCCAAGAAGC CGCCGGCCAA ACTGCAGCCC CGAGCCCTAG AAGGCTCCCT TGGCCCAGAA 
GACACAAGTC AAGTGGAAGA GGCAGAGGAT GAGCTGGAAA TCCGGTTCAA TGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGCCTCAG TACCACCAGC ATGGCCAGGC ACTCGGGAAG 
TTTCTTCAAG AGGTTCTTTG GGAAGACACC AACGGTAAGT CCTTGCCCCA CTCAGGTCAT 
TTCAGTTTCC TTGGAGTCCC AATTGTGAGC CCAACCTATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977005.1
CDS	144..548
Translation	MPSLGTMCSLLLFSVLWVDLAMAGSSFLSPEHQKLQQRKESKKPPAKLQPRALEGSLGPEDTSQVEEAEDELEIRFNAPFDVGIKLSGPQYHQHGQALGKFLQEVLWEDTNGKSLPHSGHFSFLGVPIVSPTYV

Target ORF information:

RefSeq Version	XM_014121530.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121530.1

ORF Insert Sequence:

ATGCCCTCCC TGGGGACCAT GTGCAGCCTG CTGCTCTTCA GTGTGCTCTG GGTGGACCTG 
GCCATGGCGG GCTCCAGCTT CCTAAGTCCC GAACACCAGA AACTACAGCA GAGAAAGGAG 
TCCAAGAAGC CGCCGGCCAA ACTGCAGCCC CGAGCCCTAG AAGGCTCCCT TGGCCCAGAA 
GACACAAGTC AAGTGGAAGA GGCAGAGGAT GAGCTGGAAA TCCGGTTCAA TGCCCCCTTT 
GATGTTGGAA TCAAGCTGTC AGGGCCTCAG TACCACCAGC ATGGCCAGGC ACTCGGGAAG 
TTTCTTCAAG AGGTTCTTTG GGAAGACACC AACGGTAAGT CCTTGCCCCA CTCAGGTCAT 
TTCAGTTTCC TTGGAGTCCC AATTGTGAGC CCAACCTATG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_014121531.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013977006.1 Canis lupus familiaris ghrelin/obestatin prepropeptide (GHRL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe41443
Clone ID Related Accession (Same CDS sequence)	XM_014121531.1
Accession Version	XM_014121531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	appetite-regulating hormone isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726095.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCC TGGGGACCAT GTGCAGCCTG CTGCTCTTCA GTGTGCTCTG GGTGGACCTG 
GCCATGGCGG GCTCCAGCTT CCTAAGTCCC GAACACCAGA AACTACAGAG AAAGGAGTCC 
AAGAAGCCGC CGGCCAAACT GCAGCCCCGA GCCCTAGAAG GCTCCCTTGG CCCAGAAGAC 
ACAAGTCAAG TGGAAGAGGC AGAGGATGAG CTGGAAATCC GGTTCAATGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GCCTCAGTAC CACCAGCATG GCCAGGCACT CGGGAAGTTT 
CTTCAAGAGG TTCTTTGGGA AGACACCAAC GGTAAGTCCT TGCCCCACTC AGGTCATTTC 
AGTTTCCTTG GAGTCCCAAT TGTGAGCCCA ACCTATGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013977006.1
CDS	144..545
Translation	MPSLGTMCSLLLFSVLWVDLAMAGSSFLSPEHQKLQRKESKKPPAKLQPRALEGSLGPEDTSQVEEAEDELEIRFNAPFDVGIKLSGPQYHQHGQALGKFLQEVLWEDTNGKSLPHSGHFSFLGVPIVSPTYV

Target ORF information:

RefSeq Version	XM_014121531.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ghrelin/obestatin prepropeptide (GHRL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121531.1

ORF Insert Sequence:

ATGCCCTCCC TGGGGACCAT GTGCAGCCTG CTGCTCTTCA GTGTGCTCTG GGTGGACCTG 
GCCATGGCGG GCTCCAGCTT CCTAAGTCCC GAACACCAGA AACTACAGAG AAAGGAGTCC 
AAGAAGCCGC CGGCCAAACT GCAGCCCCGA GCCCTAGAAG GCTCCCTTGG CCCAGAAGAC 
ACAAGTCAAG TGGAAGAGGC AGAGGATGAG CTGGAAATCC GGTTCAATGC CCCCTTTGAT 
GTTGGAATCA AGCTGTCAGG GCCTCAGTAC CACCAGCATG GCCAGGCACT CGGGAAGTTT 
CTTCAAGAGG TTCTTTGGGA AGACACCAAC GGTAAGTCCT TGCCCCACTC AGGTCATTTC 
AGTTTCCTTG GAGTCCCAAT TGTGAGCCCA ACCTATGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The gut hormone ghrelin partially reverses energy substrate metabolic alterations in the failing heart.
Circulation. Heart failure7(4)643-51(2014 Jul)
Mitacchione G,Powers JC,Grifoni G,Woitek F,Lam A,Ly L,Settanni F,Makarewich CA,McCormick R,Trovato L,Houser SR,Granata R,Recchia FA

A role of ghrelin in canine mammary carcinoma cells proliferation, apoptosis and migration.
BMC veterinary research8170(2012 Sep)
Majchrzak K,Paw?owski KM,Orzechowska EJ,Dolka I,Mucha J,Motyl T,Kr?l M

Coronary vasoconstrictor effect of ghrelin is not mediated by growth hormone secretagogue receptor 1a type in dogs.
Peptides32(2)362-7(2011 Feb)
Sax B,Nadasy GL,Turi K,Hirschberg K,Furjesz D,Nagy A,Merkely B,Szabo G,Monos E,Kekesi V

Identification of cDNA encoding motilin related peptide/ghrelin precursor from dog fundus.
Peptides22(12)2055-9(2001 Dec)
Tomasetto C,Wendling C,Rio MC,Poitras P

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GHRL cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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