TNF cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNF
Entrez Gene ID	280943
Full Name	tumor necrosis factor
Synonyms	TNF-a,TNF-alpha,TNFa
General protein information	Preferred Names tumor necrosis factor Names tumor necrosis factor cachectin tumor necrosis factor alpha tumor necrosis factor ligand superfamily member 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 23 Map Location: 23q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_005223596.3	XP_005223653.3	tumor necrosis factor isoform X1
XM_005223596.4	XP_005223653.4	tumor necrosis factor isoform X1
NM_173966.3	NP_776391.2	tumor necrosis factor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tnf	NP_038721.1
Canis lupus familiaris (dog)	TNF	NP_001003244.4
Bos taurus (cattle)	TNF	NP_776391.2
Rattus norvegicus (Norway rat)	Tnf	NP_036807.1
	TNF	NP_001040614.1
Xenopus tropicalis (tropical clawed frog)	tnf	NP_001107143.1
Homo sapiens (human)	TNF	NP_000585.2
Pan troglodytes (chimpanzee)	TNF	NP_001038976.1

Gene Ontology
Bibliography

Related articles in PubMed

Association of TNF-? gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads.
Lendez PA, Passucci JA, Poli MA, Gutierrez SE, Dolcini GL, Ceriani MC
Archives of virology160(8)2001-7(2015 Aug)

Early embryonic and endometrial regulation of tumor necrosis factor and tumor necrosis factor receptor 2 in the cattle uterus.
Correia-?lvarez E, G?mez E, Mart?n D, Carrocera S, P?rez S, Peynot N, Giraud-Delville C, Caama?o JN, Balseiro A, Sandra O, Duranthon V, Mu?oz M
Theriogenology83(6)1028-37(2015 Apr)

Association and expression analysis of single nucleotide polymorphisms of partial tumor necrosis factor alpha gene with mastitis in crossbred cattle.
Ranjan S, Bhushan B, Panigrahi M, Kumar A, Deb R, Kumar P, Sharma D
Animal biotechnology26(2)98-104(2015)

Tumor necrosis factor-alpha (TNF?) gene polymorphism and expression of membrane-bound TNF? protein on CD11b+ and IgM+ cells in cows naturally infected with bovine leukemia virus.
Bojaroj?-Nosowicz B, Kaczmarczyk E, Stachura A, Kubi?ska M
Polish journal of veterinary sciences18(3)533-9(2015)

Expression of matrix metalloproteinases in bovine luteal cells induced by prostaglandin F2?, interferon ? and tumor necrosis factor ?.
Abe H, Sakumoto R, Okuda K
The Journal of reproduction and development61(4)277-86(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

There was a significant association between the proviral load and a low frequency of the G/G genotype of TNF-alpha at position -824.
Title: Association of TNF-? gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads.

These results suggest that the endometrium might lower the TNF concentration in the blastocyst by (1) regulating TNF secretion into the uterine fluid and (2) inducing decreased TNF and TNFR2 mRNA transcription in the embryo.
Title: Early embryonic and endometrial regulation of tumor necrosis factor and tumor necrosis factor receptor 2 in the cattle uterus.

Studied genotypic and expression profiling of partial TNF-alpha gene and its association with mastitis susceptibility in 129 crossbred cattle.
Title: Association and expression analysis of single nucleotide polymorphisms of partial tumor necrosis factor alpha gene with mastitis in crossbred cattle.

These results suggest that polymorphism of the TNFalpha-824 A>G gene and mTNFalpha protein expression play an important role in the pathogenesis of enzootic bovine leukosis.
Title: Tumor necrosis factor-alpha (TNF?) gene polymorphism and expression of membrane-bound TNF? protein on CD11b+ and IgM+ cells in cows naturally infected with bovine leukemia virus.

Data suggest that luteolytic factors (such as TNFa, interferon gamma, and PGF2a) control expression of MMP1, other matrix metalloproteinases, and tissue inhibitors of metalloproteinase in cultured luteal cells.
Title: Expression of matrix metalloproteinases in bovine luteal cells induced by prostaglandin F2?, interferon ? and tumor necrosis factor ?.

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01529	NM_173966.3 Latest version!	Bos taurus tumor necrosis factor (TNF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa45038	XM_005223596.4 Latest version!	Bos taurus tumor necrosis factor (TNF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OBa94586	XM_005223596.3 Latest version!	Bos taurus tumor necrosis factor (TNF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNF ( NM_173966.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776391.2 Bos taurus tumor necrosis factor (TNF), mRNA.

Price & Availability↑

CloneID	OBa01529
Clone ID Related Accession (Same CDS sequence)	NM_173966.3
Accession Version	NM_173966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	tumor necrosis factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134755.1. On Aug 30, 2012 this sequence version replaced NM_173966.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC134755.1, JN635499.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04335740, SAMN05861845 [ECO:0000348] ##Evidence-Data-END##

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCGAGAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TTGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGCAGT CCCCAGGTGG CCCCTCCATC AACAGCCCTC TGGTTCAAAC ACTCAGGTCC 
TCTTCTCAAG CCTCAAGTAA CAAGCCGGTA GCCCACGTTG TAGCCGACAT CAACTCTCCG 
GGGCAGCTCC GGTGGTGGGA CTCGTATGCC AATGCCCTCA TGGCCAACGG TGTGAAGCTG 
GAAGACAACC AGCTGGTGGT GCCTGCTGAC GGGCTTTACC TCATCTACTC ACAGGTCCTC 
TTCAGGGGCC AAGGCTGCCC TTCCACCCCC TTGTTCCTCA CCCACACCAT CAGCCGCATT 
GCAGTCTCCT ACCAGACCAA GGTCAACATC CTGTCTGCCA TCAAGAGCCC TTGCCACAGG 
GAGACCCCAG AGTGGGCTGA GGCCAAGCCC TGGTATGAAC CCATCTACCA GGGAGGAGTC 
TTCCAGCTGG AGAAGGGAGA TCGCCTCAGT GCTGAGATCA ACCTGCCGGA CTACCTGGAC 
TATGCCGAGT CTGGGCAGGT CTACTTTGGG ATCATCGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776391.2
CDS	194..898
Translation	MSTKSMIRDVELAEEVLSEKAGGPQGSRSCLCLSLFSFLLVAGATTLFCLLHFGVIGPQREEQSPGGPSINSPLVQTLRSSSQASSNKPVAHVVADINSPGQLRWWDSYANALMANGVKLEDNQLVVPADGLYLIYSQVLFRGQGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETPEWAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPDYLDYAESGQVYFGIIAL

Target ORF information:

RefSeq Version	NM_173966.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173966.3

ORF Insert Sequence:

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCGAGAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TTGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGCAGT CCCCAGGTGG CCCCTCCATC AACAGCCCTC TGGTTCAAAC ACTCAGGTCC 
TCTTCTCAAG CCTCAAGTAA CAAGCCGGTA GCCCACGTTG TAGCCGACAT CAACTCTCCG 
GGGCAGCTCC GGTGGTGGGA CTCGTATGCC AATGCCCTCA TGGCCAACGG TGTGAAGCTG 
GAAGACAACC AGCTGGTGGT GCCTGCTGAC GGGCTTTACC TCATCTACTC ACAGGTCCTC 
TTCAGGGGCC AAGGCTGCCC TTCCACCCCC TTGTTCCTCA CCCACACCAT CAGCCGCATT 
GCAGTCTCCT ACCAGACCAA GGTCAACATC CTGTCTGCCA TCAAGAGCCC TTGCCACAGG 
GAGACCCCAG AGTGGGCTGA GGCCAAGCCC TGGTATGAAC CCATCTACCA GGGAGGAGTC 
TTCCAGCTGG AGAAGGGAGA TCGCCTCAGT GCTGAGATCA ACCTGCCGGA CTACCTGGAC 
TATGCCGAGT CTGGGCAGGT CTACTTTGGG ATCATCGCCC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNF ( XM_005223596.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223653.4 Bos taurus tumor necrosis factor (TNF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45038
Clone ID Related Accession (Same CDS sequence)	XM_005223596.4
Accession Version	XM_005223596.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tumor necrosis factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223596.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCGAGAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TTGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGTCCC CAGGTGGCCC CTCCATCAAC AGCCCTCTGG TTCAAACACT CAGGTCCTCT 
TCTCAAGCCT CAAGTAACAA GCCGGTAGCC CACGTTGTAG CCGACATCAA CTCTCCGGGG 
CAGCTCCGGT GGTGGGACTC GTATGCCAAT GCCCTCATGG CCAACGGTGT GAAGCTGGAA 
GACAACCAGC TGGTGGTGCC TGCTGACGGG CTTTACCTCA TCTACTCACA GGTCCTCTTC 
AGGGGCCAAG GCTGCCCTTC CACCCCCTTG TTCCTCACCC ACACCATCAG CCGCATTGCA 
GTCTCCTACC AGACCAAGGT CAACATCCTG TCTGCCATCA AGAGCCCTTG CCACAGGGAG 
ACCCCAGAGT GGGCTGAGGC CAAGCCCTGG TATGAACCCA TCTACCAGGG AGGAGTCTTC 
CAGCTGGAGA AGGGAGATCG CCTCAGTGCT GAGATCAACC TGCCGGACTA CCTGGACTAT 
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATCGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223653.4
CDS	38..739
Translation	MSTKSMIRDVELAEEVLSEKAGGPQGSRSCLCLSLFSFLLVAGATTLFCLLHFGVIGPQREESPGGPSINSPLVQTLRSSSQASSNKPVAHVVADINSPGQLRWWDSYANALMANGVKLEDNQLVVPADGLYLIYSQVLFRGQGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETPEWAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPDYLDYAESGQVYFGIIAL

Target ORF information:

RefSeq Version	XM_005223596.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tumor necrosis factor (TNF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223596.4

ORF Insert Sequence:

ATGAGCACCA AAAGCATGAT CCGGGATGTG GAGCTGGCGG AGGAGGTGCT CTCCGAGAAA 
GCAGGGGGCC CCCAGGGCTC CAGAAGTTGC TTGTGCCTCA GCCTCTTCTC CTTCCTCCTG 
GTTGCAGGAG CCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGTCCC CAGGTGGCCC CTCCATCAAC AGCCCTCTGG TTCAAACACT CAGGTCCTCT 
TCTCAAGCCT CAAGTAACAA GCCGGTAGCC CACGTTGTAG CCGACATCAA CTCTCCGGGG 
CAGCTCCGGT GGTGGGACTC GTATGCCAAT GCCCTCATGG CCAACGGTGT GAAGCTGGAA 
GACAACCAGC TGGTGGTGCC TGCTGACGGG CTTTACCTCA TCTACTCACA GGTCCTCTTC 
AGGGGCCAAG GCTGCCCTTC CACCCCCTTG TTCCTCACCC ACACCATCAG CCGCATTGCA 
GTCTCCTACC AGACCAAGGT CAACATCCTG TCTGCCATCA AGAGCCCTTG CCACAGGGAG 
ACCCCAGAGT GGGCTGAGGC CAAGCCCTGG TATGAACCCA TCTACCAGGG AGGAGTCTTC 
CAGCTGGAGA AGGGAGATCG CCTCAGTGCT GAGATCAACC TGCCGGACTA CCTGGACTAT 
GCCGAGTCTG GGCAGGTCTA CTTTGGGATC ATCGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNF ( XM_005223596.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223653.3 Bos taurus tumor necrosis factor (TNF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa94586
Clone ID Related Accession (Same CDS sequence)	XM_005223596.3
Accession Version	XM_005223596.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tumor necrosis factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223596.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTCT CCACCAAGGA AGTTTTCCGC TGGTTGAATG AGAGCCTTTC CCCGCCCTCT 
TCTCACCCCA CAGCATATAA ATGCAGCTGT TTGCACACCC AGCCAACAGA AGCTCCCAGA 
GCGGGGACAC CAGGGGACCA GCCAGGAGAG AGACAAGCAG CTGCAGAACC CCCTGGAGAT 
AACCTCCCAG ACAACACACC CCCGAGAGAC AGCCAGGCAA CTTGCTCTCT CTCACATACC 
CTGCCACAAG GCTCTCCTGT CTCCCGTCTG GACTTGAACC CTTCTGAAAA AGACACCATG 
AGCACCAAAA GCATGATCCG GGATGTGGAG CTGGCGGAGG AGGTGCTCTC CGAGAAAGCA 
GGGGGCCCCC AGGGCTCCAG AAGTTGCTTG TGCCTCAGCC TCTTCTCCTT CCTCCTGGTT 
GCAGGAGCCA CCACGCTCTT CTGCCTGCTG CACTTCGGGG TAATCGGCCC CCAGAGGGAA 
GAGTCCCCAG GTGGCCCCTC CATCAACAGC CCTCTGGTTC AAACACTCAG GTCCTCTTCT 
CAAGCCTCAA GTAACAAGCC GGTAGCCCAC GTTGTAGCCG ACATCAACTC TCCGGGGCAG 
CTCCGGTGGT GGGACTCGTA TGCCAATGCC CTCATGGCCA ACGGTGTGAA GCTGGAAGAC 
AACCAGCTGG TGGTGCCTGC TGACGGGCTT TACCTCATCT ACTCACAGGT CCTCTTCAGG 
GGCCAAGGCT GCCCTTCCAC CCCCTTGTTC CTCACCCACA CCATCAGCCG CATTGCAGTC 
TCCTACCAGA CCAAGGTCAA CATCCTGTCT GCCATCAAGA GCCCTTGCCA CAGGGAGACC 
CCAGAGTGGG CTGAGGCCAA GCCCTGGTAT GAACCCATCT ACCAGGGAGG AGTCTTCCAG 
CTGGAGAAGG GAGATCGCCT CAGTGCTGAG ATCAACCTGC CGGACTACCT GGACTATGCC 
GAGTCTGGGC AGGTCTACTT TGGGATCATC GCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223653.3
CDS	1030..2028
Translation	MGFSTKEVFRWLNESLSPPSSHPTAYKCSCLHTQPTEAPRAGTPGDQPGERQAAAEPPGDNLPDNTPPRDSQATCSLSHTLPQGSPVSRLDLNPSEKDTMSTKSMIRDVELAEEVLSEKAGGPQGSRSCLCLSLFSFLLVAGATTLFCLLHFGVIGPQREESPGGPSINSPLVQTLRSSSQASSNKPVAHVVADINSPGQLRWWDSYANALMANGVKLEDNQLVVPADGLYLIYSQVLFRGQGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETPEWAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPDYLDYAESGQVYFGIIAL

Target ORF information:

RefSeq Version	XM_005223596.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tumor necrosis factor (TNF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223596.3

ORF Insert Sequence:

ATGGGTTTCT CCACCAAGGA AGTTTTCCGC TGGTTGAATG AGAGCCTTTC CCCGCCCTCT 
TCTCACCCCA CAGCATATAA ATGCAGCTGT TTGCACACCC AGCCAACAGA AGCTCCCAGA 
GCGGGGACAC CAGGGGACCA GCCAGGAGAG AGACAAGCAG CTGCAGAACC CCCTGGAGAT 
AACCTCCCAG ACAACACACC CCCGAGAGAC AGCCAGGCAA CTTGCTCTCT CTCACATACC 
CTGCCACAAG GCTCTCCTGT CTCCCGTCTG GACTTGAACC CTTCTGAAAA AGACACCATG 
AGCACCAAAA GCATGATCCG GGATGTGGAG CTGGCGGAGG AGGTGCTCTC CGAGAAAGCA 
GGGGGCCCCC AGGGCTCCAG AAGTTGCTTG TGCCTCAGCC TCTTCTCCTT CCTCCTGGTT 
GCAGGAGCCA CCACGCTCTT CTGCCTGCTG CACTTCGGGG TAATCGGCCC CCAGAGGGAA 
GAGTCCCCAG GTGGCCCCTC CATCAACAGC CCTCTGGTTC AAACACTCAG GTCCTCTTCT 
CAAGCCTCAA GTAACAAGCC GGTAGCCCAC GTTGTAGCCG ACATCAACTC TCCGGGGCAG 
CTCCGGTGGT GGGACTCGTA TGCCAATGCC CTCATGGCCA ACGGTGTGAA GCTGGAAGAC 
AACCAGCTGG TGGTGCCTGC TGACGGGCTT TACCTCATCT ACTCACAGGT CCTCTTCAGG 
GGCCAAGGCT GCCCTTCCAC CCCCTTGTTC CTCACCCACA CCATCAGCCG CATTGCAGTC 
TCCTACCAGA CCAAGGTCAA CATCCTGTCT GCCATCAAGA GCCCTTGCCA CAGGGAGACC 
CCAGAGTGGG CTGAGGCCAA GCCCTGGTAT GAACCCATCT ACCAGGGAGG AGTCTTCCAG 
CTGGAGAAGG GAGATCGCCT CAGTGCTGAG ATCAACCTGC CGGACTACCT GGACTATGCC 
GAGTCTGGGC AGGTCTACTT TGGGATCATC GCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association of TNF-? gene promoter region polymorphisms in bovine leukemia virus (BLV)-infected cattle with different proviral loads.
Archives of virology160(8)2001-7(2015 Aug)
Lendez PA,Passucci JA,Poli MA,Gutierrez SE,Dolcini GL,Ceriani MC

Early embryonic and endometrial regulation of tumor necrosis factor and tumor necrosis factor receptor 2 in the cattle uterus.
Theriogenology83(6)1028-37(2015 Apr)
Correia-?lvarez E,G?mez E,Mart?n D,Carrocera S,P?rez S,Peynot N,Giraud-Delville C,Caama?o JN,Balseiro A,Sandra O,Duranthon V,Mu?oz M

Association and expression analysis of single nucleotide polymorphisms of partial tumor necrosis factor alpha gene with mastitis in crossbred cattle.
Animal biotechnology26(2)98-104(2015)
Ranjan S,Bhushan B,Panigrahi M,Kumar A,Deb R,Kumar P,Sharma D

Tumor necrosis factor-alpha (TNF?) gene polymorphism and expression of membrane-bound TNF? protein on CD11b+ and IgM+ cells in cows naturally infected with bovine leukemia virus.
Polish journal of veterinary sciences18(3)533-9(2015)
Bojaroj?-Nosowicz B,Kaczmarczyk E,Stachura A,Kubi?ska M

Expression of matrix metalloproteinases in bovine luteal cells induced by prostaglandin F2?, interferon ? and tumor necrosis factor ?.
The Journal of reproduction and development61(4)277-86(2015)
Abe H,Sakumoto R,Okuda K

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TNF cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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