Tnf cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnf
Entrez Gene ID	21926
Full Name	tumor necrosis factor
Synonyms	DIF,TNF-a,TNF-alpha,TNFSF2,TNFalpha,Tnfa,Tnfsf1a,Tnlg1f
General protein information	Preferred Names tumor necrosis factor Names tumor necrosis factor cachectin tumor necrosis factor ligand 1f tumor necrosis factor ligand superfamily member 2 tumor necrosis factor-alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 B1\|17 18.59 cM
Summary	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013693.3	NP_038721.1	tumor necrosis factor isoform 1
NM_001278601.1	NP_001265530.1	tumor necrosis factor isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tnf	NP_038721.1
Canis lupus familiaris (dog)	TNF	NP_001003244.4
Bos taurus (cattle)	TNF	NP_776391.2
Rattus norvegicus (Norway rat)	Tnf	NP_036807.1
	TNF	NP_001040614.1
Xenopus tropicalis (tropical clawed frog)	tnf	NP_001107143.1
Homo sapiens (human)	TNF	NP_000585.2
Pan troglodytes (chimpanzee)	TNF	NP_001038976.1

Gene Ontology
Bibliography

Related articles in PubMed

Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents.
Horiuchi T, Mitoma H, Harashima S, Tsukamoto H, Shimoda T
Rheumatology (Oxford, England)49(7)1215-28(2010 Jul)

The structure of mouse tumour-necrosis factor at 1.4 A resolution: towards modulation of its selectivity and trimerization.
Baeyens KJ, De Bondt HL, Raeymaekers A, Fiers W, De Ranter CJ
Acta crystallographica. Section D, Biological crystallography55(Pt 4)772-8(1999 Apr)

Crystallization and preliminary X-ray studies of mouse tumor necrosis factor.
Baeyens KJ, De Bondt HL, Raeymaekers A, De Ranter CJ, Fiers W
Acta crystallographica. Section D, Biological crystallography53(Pt 3)329-30(1997 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that the SH3BP2 cherubism mutation can cause jawbone destruction by promoting osteoclast formation in response to TNF-alpha expressed in cherubism lesions and that SH3BP2 is a key regulator for TNF-alpha-induced osteoclastogenesis
Title: SH3BP2 cherubism mutation potentiates TNF-?-induced osteoclastogenesis via NFATc1 and TNF-?-mediated inflammatory bone loss.

These results demonstrate that MMP-8 critically mediates microglial activation by modulating TNF-alpha activity, which may explain neuroinflammation in septic mouse brain.
Title: Matrix metalloproteinase-8 plays a pivotal role in neuroinflammation by modulating TNF-? activation.

TNFalpha in humans demonstrates 550 My of functional conservation by binding directly to coral cells, increasing caspase activity, causing apoptotic blebbing and cell death, and finally inducing coral bleaching.
Title: Evolution of TNF-induced apoptosis reveals 550 My of functional conservation.

Data indicate that blocking TNF-alpha and IL-1beta simultaneously with TNF receptor 2-fragment crystalization-IL-1 receptor antagonist (TFI) rather effectively reduced Ang II-induced cardiac damage.
Title: 5TNF-? and IL-1? neutralization ameliorates angiotensin II-induced cardiac damage in male mice.

Etanercept administration to neonatal SH3BP2 knock-in cherubism mice prevents TNF-alpha-induced inflammation and bone loss.
Title: Etanercept administration to neonatal SH3BP2 knock-in cherubism mice prevents TNF-?-induced inflammation and bone loss.

The following Tnf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23207	NM_001278601.1 Latest version!	Mus musculus tumor necrosis factor (Tnf), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu20592	NM_013693.3 Latest version!	Mus musculus tumor necrosis factor (Tnf), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnf ( NM_001278601.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001265530.1 Mus musculus tumor necrosis factor (Tnf), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23207
Clone ID Related Accession (Same CDS sequence)	NM_001278601.1
Accession Version	NM_001278601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	tumor necrosis factor isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CR974444.18 and BY167004.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154223.1, BY747796.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCACAG AAAGCATGAT CCGCGACGTG GAACTGGCAG AAGAGGCACT CCCCCAAAAG 
ATGGGGGGCT TCCAGAACTC CAGGCGGTGC CTATGTCTCA GCCTCTTCTC ATTCCTGCTT 
GTGGCAGGGG CCACCACGCT CTTCTGTCTA CTGAACTTCG GGGTGATCGG TCCCCAAAGG 
GATGAGAAGT TCCCAAATGG CCTCCCTCTC ATCAGTTCTA TGGCCCAGAC CCTCACACTC 
ACAAACCACC AAGTGGAGGA GCAGCTGGAG TGGCTGAGCC AGCGCGCCAA CGCCCTCCTG 
GCCAACGGCA TGGATCTCAA AGACAACCAA CTAGTGGTGC CAGCCGATGG GTTGTACCTT 
GTCTACTCCC AGGTTCTCTT CAAGGGACAA GGCTGCCCCG ACTACGTGCT CCTCACCCAC 
ACCGTCAGCC GATTTGCTAT CTCATACCAG GAGAAAGTCA ACCTCCTCTC TGCCGTCAAG 
AGCCCCTGCC CCAAGGACAC CCCTGAGGGG GCTGAGCTCA AACCCTGGTA TGAGCCCATA 
TACCTGGGAG GAGTCTTCCA GCTGGAGAAG GGGGACCAAC TCAGCGCTGA GGTCAATCTG 
CCCAAGTACT TAGACTTTGC GGAGTCCGGG CAGGTCTACT TTGGAGTCAT TGCTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265530.1
CDS	168..827
Translation	MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPLISSMAQTLTLTNHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL

Target ORF information:

RefSeq Version	NM_001278601.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tumor necrosis factor (Tnf), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278601.1

ORF Insert Sequence:

ATGAGCACAG AAAGCATGAT CCGCGACGTG GAACTGGCAG AAGAGGCACT CCCCCAAAAG 
ATGGGGGGCT TCCAGAACTC CAGGCGGTGC CTATGTCTCA GCCTCTTCTC ATTCCTGCTT 
GTGGCAGGGG CCACCACGCT CTTCTGTCTA CTGAACTTCG GGGTGATCGG TCCCCAAAGG 
GATGAGAAGT TCCCAAATGG CCTCCCTCTC ATCAGTTCTA TGGCCCAGAC CCTCACACTC 
ACAAACCACC AAGTGGAGGA GCAGCTGGAG TGGCTGAGCC AGCGCGCCAA CGCCCTCCTG 
GCCAACGGCA TGGATCTCAA AGACAACCAA CTAGTGGTGC CAGCCGATGG GTTGTACCTT 
GTCTACTCCC AGGTTCTCTT CAAGGGACAA GGCTGCCCCG ACTACGTGCT CCTCACCCAC 
ACCGTCAGCC GATTTGCTAT CTCATACCAG GAGAAAGTCA ACCTCCTCTC TGCCGTCAAG 
AGCCCCTGCC CCAAGGACAC CCCTGAGGGG GCTGAGCTCA AACCCTGGTA TGAGCCCATA 
TACCTGGGAG GAGTCTTCCA GCTGGAGAAG GGGGACCAAC TCAGCGCTGA GGTCAATCTG 
CCCAAGTACT TAGACTTTGC GGAGTCCGGG CAGGTCTACT TTGGAGTCAT TGCTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnf ( NM_013693.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038721.1 Mus musculus tumor necrosis factor (Tnf), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20592
Clone ID Related Accession (Same CDS sequence)	NM_013693.3
Accession Version	NM_013693.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	tumor necrosis factor isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK153800.1, AK153319.1 and CR974444.18. On Jun 30, 2013 this sequence version replaced NM_013693.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK153319.1, AK153800.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCACAG AAAGCATGAT CCGCGACGTG GAACTGGCAG AAGAGGCACT CCCCCAAAAG 
ATGGGGGGCT TCCAGAACTC CAGGCGGTGC CTATGTCTCA GCCTCTTCTC ATTCCTGCTT 
GTGGCAGGGG CCACCACGCT CTTCTGTCTA CTGAACTTCG GGGTGATCGG TCCCCAAAGG 
GATGAGAAGT TCCCAAATGG CCTCCCTCTC ATCAGTTCTA TGGCCCAGAC CCTCACACTC 
AGATCATCTT CTCAAAATTC GAGTGACAAG CCTGTAGCCC ACGTCGTAGC AAACCACCAA 
GTGGAGGAGC AGCTGGAGTG GCTGAGCCAG CGCGCCAACG CCCTCCTGGC CAACGGCATG 
GATCTCAAAG ACAACCAACT AGTGGTGCCA GCCGATGGGT TGTACCTTGT CTACTCCCAG 
GTTCTCTTCA AGGGACAAGG CTGCCCCGAC TACGTGCTCC TCACCCACAC CGTCAGCCGA 
TTTGCTATCT CATACCAGGA GAAAGTCAAC CTCCTCTCTG CCGTCAAGAG CCCCTGCCCC 
AAGGACACCC CTGAGGGGGC TGAGCTCAAA CCCTGGTATG AGCCCATATA CCTGGGAGGA 
GTCTTCCAGC TGGAGAAGGG GGACCAACTC AGCGCTGAGG TCAATCTGCC CAAGTACTTA 
GACTTTGCGG AGTCCGGGCA GGTCTACTTT GGAGTCATTG CTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038721.1
CDS	168..875
Translation	MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPLISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL

Target ORF information:

RefSeq Version	NM_013693.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tumor necrosis factor (Tnf), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013693.3

ORF Insert Sequence:

ATGAGCACAG AAAGCATGAT CCGCGACGTG GAACTGGCAG AAGAGGCACT CCCCCAAAAG 
ATGGGGGGCT TCCAGAACTC CAGGCGGTGC CTATGTCTCA GCCTCTTCTC ATTCCTGCTT 
GTGGCAGGGG CCACCACGCT CTTCTGTCTA CTGAACTTCG GGGTGATCGG TCCCCAAAGG 
GATGAGAAGT TCCCAAATGG CCTCCCTCTC ATCAGTTCTA TGGCCCAGAC CCTCACACTC 
AGATCATCTT CTCAAAATTC GAGTGACAAG CCTGTAGCCC ACGTCGTAGC AAACCACCAA 
GTGGAGGAGC AGCTGGAGTG GCTGAGCCAG CGCGCCAACG CCCTCCTGGC CAACGGCATG 
GATCTCAAAG ACAACCAACT AGTGGTGCCA GCCGATGGGT TGTACCTTGT CTACTCCCAG 
GTTCTCTTCA AGGGACAAGG CTGCCCCGAC TACGTGCTCC TCACCCACAC CGTCAGCCGA 
TTTGCTATCT CATACCAGGA GAAAGTCAAC CTCCTCTCTG CCGTCAAGAG CCCCTGCCCC 
AAGGACACCC CTGAGGGGGC TGAGCTCAAA CCCTGGTATG AGCCCATATA CCTGGGAGGA 
GTCTTCCAGC TGGAGAAGGG GGACCAACTC AGCGCTGAGG TCAATCTGCC CAAGTACTTA 
GACTTTGCGG AGTCCGGGCA GGTCTACTTT GGAGTCATTG CTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents.
Rheumatology (Oxford, England)49(7)1215-28(2010 Jul)
Horiuchi T,Mitoma H,Harashima S,Tsukamoto H,Shimoda T

The structure of mouse tumour-necrosis factor at 1.4 A resolution: towards modulation of its selectivity and trimerization.
Acta crystallographica. Section D, Biological crystallography55(Pt 4)772-8(1999 Apr)
Baeyens KJ,De Bondt HL,Raeymaekers A,Fiers W,De Ranter CJ

Crystallization and preliminary X-ray studies of mouse tumor necrosis factor.
Acta crystallographica. Section D, Biological crystallography53(Pt 3)329-30(1997 May)
Baeyens KJ,De Bondt HL,Raeymaekers A,De Ranter CJ,Fiers W

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Tnf cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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