KDM6B cDNA ORF clone, Bos taurus(cattle)

The following KDM6B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM6B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa226773 XM_010816061.3
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226773 XM_024980740.1
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226773 XM_010816059.2
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226773 XM_010816062.3
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226773 XM_015458746.2
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226773 XM_003587412.5
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226773 XM_024980742.1
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226773 XM_010816060.3
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226773 XM_024980741.1
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226773 XM_010816063.3
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226773 XM_015458745.2
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa226774 XM_024980743.1
Latest version!
Bos taurus lysine demethylase 6B (KDM6B), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa29804 XM_015458745.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015458746.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_010816059.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015468041.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015458747.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015468042.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015468036.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_010816063.2
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015468038.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015468035.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015468034.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_010816061.2
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015468039.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_010816062.2
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_010816060.2
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_003587412.4
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015468040.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa29804 XM_015468037.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa93888 XM_015458748.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa93888 XM_015468043.1
Latest version!
Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_010816060.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816060.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814362.2
    CDS792..5726
    Translation

    Target ORF information:

    RefSeq Version XM_010816060.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816060.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_010816062.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816062.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814364.2
    CDS539..5473
    Translation

    Target ORF information:

    RefSeq Version XM_010816062.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816062.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_010816063.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816063.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814365.2
    CDS421..5355
    Translation

    Target ORF information:

    RefSeq Version XM_010816063.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816063.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_024980742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836510.1
    CDS443..5377
    Translation

    Target ORF information:

    RefSeq Version XM_024980742.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_015458745.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314231.2
    CDS424..5358
    Translation

    Target ORF information:

    RefSeq Version XM_015458745.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458745.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_010816059.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814361.2
    CDS468..5402
    Translation

    Target ORF information:

    RefSeq Version XM_010816059.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816059.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_010816061.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816061.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814363.2
    CDS412..5346
    Translation

    Target ORF information:

    RefSeq Version XM_010816061.3
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816061.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_024980741.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836509.1
    CDS788..5722
    Translation

    Target ORF information:

    RefSeq Version XM_024980741.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980741.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_015458746.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314232.2
    CDS1351..6285
    Translation

    Target ORF information:

    RefSeq Version XM_015458746.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458746.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_003587412.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587412.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587460.3
    CDS543..5477
    Translation

    Target ORF information:

    RefSeq Version XM_003587412.5
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587412.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226773
    Clone ID Related Accession (Same CDS sequence) XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
    Accession Version XM_024980740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836508.1
    CDS464..5398
    Translation

    Target ORF information:

    RefSeq Version XM_024980740.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC
    ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC
    CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC
    AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT
    GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC
    TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA
    CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG
    CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC
    CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT
    GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA
    GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG
    AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC
    CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT
    GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG
    AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT
    GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT
    GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA
    GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC
    CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC
    GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT
    GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC
    CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA
    AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG
    GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA
    GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG
    GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG
    ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG
    TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG
    GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226774
    Clone ID Related Accession (Same CDS sequence) XM_024980743.1
    Accession Version XM_024980743.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GGAGCAGCGG
    CACTCGCTGC CTCACTCATA TCCATACCCG GCTCCTGCTT ACCCTGCTCA CCCCCCTGGC
    CACCGGCTGG TCCCGGCTGC TCCCCCAGGC CCAGGCCCCC GCCCCCCAGG AGCAGAGAGC
    CATGGCTGCC CGCCTGCCAC CCGTCCCCCC GGAAGTGACC TTAGAGAGAG CAGAGTTCAG
    AGGTCGCGGA TGGACTCCAG CGTTTCACCA GCAGCAACCA CCGCCTGCGT GCCTTACGCC
    CCTTCCCGGC CCCCCGGCCT CCCCGGCACC ACCAGCAGCA GCAGCAGCAA CACTGGTCCC
    CGTGGCGTGG AGCCGAGCCC AGGCATTCCC GGCGCTGACC ATTACCAGAC ACCCGCGCTG
    GAGGTCTCCT CTCACCAAGG CCGCCTGGGG CCCTCGGCAC ACAGTAGTCG GAAACCCTTC
    CTGGCGGCTC CTGCTGCCAC TCCCCACCTG TCTCTGCCAC CTGGCCCCAC CTCACCTCCT
    CCACCCTCTT GTCCCCGCCT CATACGCCCC CCACCCCCTC CTGCCTGGCT GAAGGGCCCA
    GCCTGCCGGG CAGCTCGTGA AGATGGAGAG ATCTTAGAGG AGCTCTTCTT CGGGGCTGAG
    GGACGCCCCC GCCCTCCCCC CCCTCCACTT CCCCACCGCG AGGGCTTCTT GGGGCCTCCG
    GCCCCCCGCT TTTCTGTGGG CACTCAGGAT TCGCACACCC CTCCCACTCC CCCAACCACC
    AGCAGCAGCA GCAGCAACAA TGGCAGCCAC AGCAGCAGCC CTACTGGGCC TGTGTCCTTC
    CCCCCACCTC CTTATCTGGC CAGAAGTATG GACTCCCTTC CCCGGCCCCC CAGCCCCACA
    CTGAGCCCCC AGGACCCACC CCTTGCACCC CTGACTCTTG CCCTGCCTTC AGCCCCTCCC
    TCCTCTTGCC ACCAAAATAC CTCAGGAAGC TTCAGGCGCC CGGAGAGCCC TCGGCCCAGG
    GTCTCCTTCC CAAAGACCCC CGAGGTGGGG CCGGGGCCAT CCCCAGGCCC CCTGAATAAA
    ACCCCCCAGC CTGTGCCGCC CAGGGTCGGG GAGCTGCCTG CCCGAGGCCC TCGACTTTTT
    GATTTCCCTC CTACCCCTCT GGAGGATCAG TTTGAGGAGC CAGCTGAATT CAAGATCCTA
    CCTGATGGGC TGGCTAATAT CATGAAGATG CTGGATGAAT CCATTCGCAA GGAGGAGGAG
    CAGCAACAAC AGGAGGCAGG CGCGGTCCCC GTCCCCCCGC CTCCTCTGAA GGAGCCCTTT
    GCATCTCTGC AGCCTCCGTT CCCCACAGAC ACAGCCCCAG CCACCACTGC TGCCGCCGCC
    GCCACCGCCA CCACCACCAC CACCACCACC ACAGCCACCC AGGAAGAGGA GAAGAAGCCA
    CCAGCCCTGC CACCACCGCC GCCTCTAGCC AAGTTCCCCC CACCACCCCA GCCACAGCCA
    CCGCCACCCC CGCTCCCCCC ACCAGCCAGC CCGGCCAGCC TGCTCAAGTC CTTGGCCTCC
    GTGCTGGAGG GACAAAAGTA CTGTTACCGA GGGACTGGAG CTGCTGTTCC CACCCGGCCT
    GGGTCCTTGC CCACCACTCA GTATTCCCCT GGTCCCTCAT CAGGTGCTAC CGCCCCGCCA
    CCCACCTCAG TGGCCCCGAG CGCCCAGGGC TCCCCACAGC CCTCCGCTTC CTCGTCATCT
    CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC CGGGAGCGCA GGGCCAGCGA AGAGCCAGCC
    CCAGGCCCCA TGGCCCCCGC CCCGCCGCCC CCACCCCTGC CTCTGCCCCC TGCTCGCTCT
    GAGTCTGAGG TGCTAGAAGA GATCAGTCGG GCTTGTGAGA CCCTTGTGGA GCGGGTAGGC
    CGGAGTGCCA CGGACCCGGC AGACCTGGTG GACACAGCAG ATGCAGCGGA CAGTGGAGCT
    GAGCGATTGC TGCCTCCAGC TCCGGCCAAG GAGGAGAGCG TTGGGGTGGC AGCTGCGGCA
    GGACCCGGGG GCAGCAAGCG GCGGCAGAAG GAGCACCAGA AAGAGCACCG GCGGCACAGG
    CGGGCCTGCA AGGACAGTGT GGGGCGGCGG CCCCGTGAGG GCAGGGCCAA GACCAAGGCC
    AAGGCCCCCA AAGAAAAGAG CCGCAGGGTG CTGGGGAACC TGGACTTGCA GAGCGAGGAG
    ATCCAGGGTC GTGAGAAAGC CCGGCCAGAT CTTGGTGGGG CCTCCAAGGC CAAGCCACCC
    ACAGCTCCAG GCCCTCCACC TGCTCCTGCC CCCTCTGCCC AGCCCACACC CCCGGCAGCC
    CCTGCCCCTG GGAAGAAAGC TCGAGAGGAA GCTTCAGGGC CACCAGGGGT CAGCCGGGCT
    GACATGCTGA AGCTGCGCTC ACTCAGCGAA GGGCCCCCCA AGGAGCTGAA GATCCGGCTC
    ATCAAGGTAG AGAGCGGTGA CAAGGAGACC TTCATCGCCT CCGAGGTGGA AGAGCGGCGG
    CTGCGGATGG CAGACCTTAC CATCAGCCAC TGTGCTGCTG ACGTTGTGCG TGCCAGCAAG
    AACGCCAAGG TGAAAGGGAA GTTTCGAGAG TCCTACCTGT CCCCTGCCCA GTCTGTGAAA
    CCGAAGATCA ACACTGAGGA AAAGCTGCCC CGGGAAAAAC TCAACCCACC CACACCTAGC
    ATCTATCTGG AAAGCAAACG GGATGCCTTC TCGCCGGTGC TGTTACAGTT CTGTACAGAC
    CCTCGAAATC CCATCACCGT GATCCGGGGC CTGGCAGGCT CCCTGCGGCT CAACTTGGGC
    CTCTTCTCCA CCAAGACTCT GGTGGAGGCG AGTGGGGAGC ACACAGTGGA GGTGCGCACC
    CAGGTGCAGC AGCCCTCGGA TGAGAACTGG GATCTGACGG GCACACGACA GATCTGGCCC
    TGCGAGAGCT CCCGTTCCCA TACCACCATT GCCAAGTACG CGCAGTACCA GGCCTCATCC
    TTCCAGGAAT CCCTGCAGGA GGAGAAGGAG AGTGAGGACG AGGAGTCAGA GGAGCCGGAC
    AGCACCACAG GAACCCCTCC TAGCAGTGCA CCAGACCCGA AGAACCATCA CATCATCAAG
    TTTGGCACCA ACATCGACCT GTCTGATGCT AAGCGGTGGA AGCCCCAGCT GCAGGAGCTA
    CTGAAGCTGC CCGCCTTCAT GCGTGTCACC TCCACGGGGA ACATGCTGAG CCACGTGGGC
    CACACCATCC TGGGCATGAA CACGGTGCAG CTGTACATGA AGGTGCCCGG CAGCCGAACG
    CCAGGCCATC AAGAGAACAA CAACTTCTGC TCGGTCAACA TCAACATTGG CCCAGGAGAC
    TGCGAGTGGT TCGCGGTGCA CGAGCACTAC TGGGAGACCA TCAGCGCCTT CTGCGACCGG
    CACGGCGTGG ACTACCTGAC GGGTTCCTGG TGGCCAATCC TGGATGACCT CTATGCTTCC
    AACATCCCTG TGTACCGCTT CGTGCAGCGC CCCGGAGACC TGGTGTGGAT TAATGCGGGC
    ACCGTGCACT GGGTGCAGGC CACCGGCTGG TGTAACAACA TCGCCTGGAA CGTGGGGCCC
    CTCACCGCCT ATCAGTACCA GCTGGCCCTG GAACGATATG AGTGGAATGA GGTGAAGAAT
    GTCAAATCCA TTGTGCCCAT GATTCACGTG TCCTGGAACG TGGCTCGCAC GGTCAAAATC
    AGCGACCCCG ACTTGTTCAA GATGATCAAG TTCTGCCTCC TGCAGTCCAT GAAGCACTGC
    CAGGTGCAGC GGGAGAGCCT GGTGCGGGCC GGGAAGAAAA TTGCCTACCA GGGCCGGGTC
    AAGGATGAGC CCGCCTACTA CTGCAACGAG TGCGACGTGG AGGTGTTCAA CATCCTGTTC
    GTGACGAGCG AGAACGGCAG CCGCAACACG TACCTGGTGC ACTGCGAGGC GTGCGCGCGG
    CGCCGCAGCG CGGGCCTGCA GGGCGTGGTG GTGTTGGAGC AATACCGCAC CGAAGAGCTG
    GCGCAGGCCT ATGACGCCTT CACGCTGGCC CCCGCCAGCA CGTCGCGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836511.1
    CDS543..5273
    Translation

    Target ORF information:

    RefSeq Version XM_024980743.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 6B (KDM6B), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GGAGCAGCGG
    CACTCGCTGC CTCACTCATA TCCATACCCG GCTCCTGCTT ACCCTGCTCA CCCCCCTGGC
    CACCGGCTGG TCCCGGCTGC TCCCCCAGGC CCAGGCCCCC GCCCCCCAGG AGCAGAGAGC
    CATGGCTGCC CGCCTGCCAC CCGTCCCCCC GGAAGTGACC TTAGAGAGAG CAGAGTTCAG
    AGGTCGCGGA TGGACTCCAG CGTTTCACCA GCAGCAACCA CCGCCTGCGT GCCTTACGCC
    CCTTCCCGGC CCCCCGGCCT CCCCGGCACC ACCAGCAGCA GCAGCAGCAA CACTGGTCCC
    CGTGGCGTGG AGCCGAGCCC AGGCATTCCC GGCGCTGACC ATTACCAGAC ACCCGCGCTG
    GAGGTCTCCT CTCACCAAGG CCGCCTGGGG CCCTCGGCAC ACAGTAGTCG GAAACCCTTC
    CTGGCGGCTC CTGCTGCCAC TCCCCACCTG TCTCTGCCAC CTGGCCCCAC CTCACCTCCT
    CCACCCTCTT GTCCCCGCCT CATACGCCCC CCACCCCCTC CTGCCTGGCT GAAGGGCCCA
    GCCTGCCGGG CAGCTCGTGA AGATGGAGAG ATCTTAGAGG AGCTCTTCTT CGGGGCTGAG
    GGACGCCCCC GCCCTCCCCC CCCTCCACTT CCCCACCGCG AGGGCTTCTT GGGGCCTCCG
    GCCCCCCGCT TTTCTGTGGG CACTCAGGAT TCGCACACCC CTCCCACTCC CCCAACCACC
    AGCAGCAGCA GCAGCAACAA TGGCAGCCAC AGCAGCAGCC CTACTGGGCC TGTGTCCTTC
    CCCCCACCTC CTTATCTGGC CAGAAGTATG GACTCCCTTC CCCGGCCCCC CAGCCCCACA
    CTGAGCCCCC AGGACCCACC CCTTGCACCC CTGACTCTTG CCCTGCCTTC AGCCCCTCCC
    TCCTCTTGCC ACCAAAATAC CTCAGGAAGC TTCAGGCGCC CGGAGAGCCC TCGGCCCAGG
    GTCTCCTTCC CAAAGACCCC CGAGGTGGGG CCGGGGCCAT CCCCAGGCCC CCTGAATAAA
    ACCCCCCAGC CTGTGCCGCC CAGGGTCGGG GAGCTGCCTG CCCGAGGCCC TCGACTTTTT
    GATTTCCCTC CTACCCCTCT GGAGGATCAG TTTGAGGAGC CAGCTGAATT CAAGATCCTA
    CCTGATGGGC TGGCTAATAT CATGAAGATG CTGGATGAAT CCATTCGCAA GGAGGAGGAG
    CAGCAACAAC AGGAGGCAGG CGCGGTCCCC GTCCCCCCGC CTCCTCTGAA GGAGCCCTTT
    GCATCTCTGC AGCCTCCGTT CCCCACAGAC ACAGCCCCAG CCACCACTGC TGCCGCCGCC
    GCCACCGCCA CCACCACCAC CACCACCACC ACAGCCACCC AGGAAGAGGA GAAGAAGCCA
    CCAGCCCTGC CACCACCGCC GCCTCTAGCC AAGTTCCCCC CACCACCCCA GCCACAGCCA
    CCGCCACCCC CGCTCCCCCC ACCAGCCAGC CCGGCCAGCC TGCTCAAGTC CTTGGCCTCC
    GTGCTGGAGG GACAAAAGTA CTGTTACCGA GGGACTGGAG CTGCTGTTCC CACCCGGCCT
    GGGTCCTTGC CCACCACTCA GTATTCCCCT GGTCCCTCAT CAGGTGCTAC CGCCCCGCCA
    CCCACCTCAG TGGCCCCGAG CGCCCAGGGC TCCCCACAGC CCTCCGCTTC CTCGTCATCT
    CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC CGGGAGCGCA GGGCCAGCGA AGAGCCAGCC
    CCAGGCCCCA TGGCCCCCGC CCCGCCGCCC CCACCCCTGC CTCTGCCCCC TGCTCGCTCT
    GAGTCTGAGG TGCTAGAAGA GATCAGTCGG GCTTGTGAGA CCCTTGTGGA GCGGGTAGGC
    CGGAGTGCCA CGGACCCGGC AGACCTGGTG GACACAGCAG ATGCAGCGGA CAGTGGAGCT
    GAGCGATTGC TGCCTCCAGC TCCGGCCAAG GAGGAGAGCG TTGGGGTGGC AGCTGCGGCA
    GGACCCGGGG GCAGCAAGCG GCGGCAGAAG GAGCACCAGA AAGAGCACCG GCGGCACAGG
    CGGGCCTGCA AGGACAGTGT GGGGCGGCGG CCCCGTGAGG GCAGGGCCAA GACCAAGGCC
    AAGGCCCCCA AAGAAAAGAG CCGCAGGGTG CTGGGGAACC TGGACTTGCA GAGCGAGGAG
    ATCCAGGGTC GTGAGAAAGC CCGGCCAGAT CTTGGTGGGG CCTCCAAGGC CAAGCCACCC
    ACAGCTCCAG GCCCTCCACC TGCTCCTGCC CCCTCTGCCC AGCCCACACC CCCGGCAGCC
    CCTGCCCCTG GGAAGAAAGC TCGAGAGGAA GCTTCAGGGC CACCAGGGGT CAGCCGGGCT
    GACATGCTGA AGCTGCGCTC ACTCAGCGAA GGGCCCCCCA AGGAGCTGAA GATCCGGCTC
    ATCAAGGTAG AGAGCGGTGA CAAGGAGACC TTCATCGCCT CCGAGGTGGA AGAGCGGCGG
    CTGCGGATGG CAGACCTTAC CATCAGCCAC TGTGCTGCTG ACGTTGTGCG TGCCAGCAAG
    AACGCCAAGG TGAAAGGGAA GTTTCGAGAG TCCTACCTGT CCCCTGCCCA GTCTGTGAAA
    CCGAAGATCA ACACTGAGGA AAAGCTGCCC CGGGAAAAAC TCAACCCACC CACACCTAGC
    ATCTATCTGG AAAGCAAACG GGATGCCTTC TCGCCGGTGC TGTTACAGTT CTGTACAGAC
    CCTCGAAATC CCATCACCGT GATCCGGGGC CTGGCAGGCT CCCTGCGGCT CAACTTGGGC
    CTCTTCTCCA CCAAGACTCT GGTGGAGGCG AGTGGGGAGC ACACAGTGGA GGTGCGCACC
    CAGGTGCAGC AGCCCTCGGA TGAGAACTGG GATCTGACGG GCACACGACA GATCTGGCCC
    TGCGAGAGCT CCCGTTCCCA TACCACCATT GCCAAGTACG CGCAGTACCA GGCCTCATCC
    TTCCAGGAAT CCCTGCAGGA GGAGAAGGAG AGTGAGGACG AGGAGTCAGA GGAGCCGGAC
    AGCACCACAG GAACCCCTCC TAGCAGTGCA CCAGACCCGA AGAACCATCA CATCATCAAG
    TTTGGCACCA ACATCGACCT GTCTGATGCT AAGCGGTGGA AGCCCCAGCT GCAGGAGCTA
    CTGAAGCTGC CCGCCTTCAT GCGTGTCACC TCCACGGGGA ACATGCTGAG CCACGTGGGC
    CACACCATCC TGGGCATGAA CACGGTGCAG CTGTACATGA AGGTGCCCGG CAGCCGAACG
    CCAGGCCATC AAGAGAACAA CAACTTCTGC TCGGTCAACA TCAACATTGG CCCAGGAGAC
    TGCGAGTGGT TCGCGGTGCA CGAGCACTAC TGGGAGACCA TCAGCGCCTT CTGCGACCGG
    CACGGCGTGG ACTACCTGAC GGGTTCCTGG TGGCCAATCC TGGATGACCT CTATGCTTCC
    AACATCCCTG TGTACCGCTT CGTGCAGCGC CCCGGAGACC TGGTGTGGAT TAATGCGGGC
    ACCGTGCACT GGGTGCAGGC CACCGGCTGG TGTAACAACA TCGCCTGGAA CGTGGGGCCC
    CTCACCGCCT ATCAGTACCA GCTGGCCCTG GAACGATATG AGTGGAATGA GGTGAAGAAT
    GTCAAATCCA TTGTGCCCAT GATTCACGTG TCCTGGAACG TGGCTCGCAC GGTCAAAATC
    AGCGACCCCG ACTTGTTCAA GATGATCAAG TTCTGCCTCC TGCAGTCCAT GAAGCACTGC
    CAGGTGCAGC GGGAGAGCCT GGTGCGGGCC GGGAAGAAAA TTGCCTACCA GGGCCGGGTC
    AAGGATGAGC CCGCCTACTA CTGCAACGAG TGCGACGTGG AGGTGTTCAA CATCCTGTTC
    GTGACGAGCG AGAACGGCAG CCGCAACACG TACCTGGTGC ACTGCGAGGC GTGCGCGCGG
    CGCCGCAGCG CGGGCCTGCA GGGCGTGGTG GTGTTGGAGC AATACCGCAC CGAAGAGCTG
    GCGCAGGCCT ATGACGCCTT CACGCTGGCC CCCGCCAGCA CGTCGCGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015458745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314231.1
    CDS416..5353
    Translation

    Target ORF information:

    RefSeq Version XM_015458745.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015458746.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314232.1
    CDS1359..6296
    Translation

    Target ORF information:

    RefSeq Version XM_015458746.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458746.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015458747.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314233.1
    CDS648..5585
    Translation

    Target ORF information:

    RefSeq Version XM_015458747.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015468042.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323528.1
    CDS648..5585
    Translation

    Target ORF information:

    RefSeq Version XM_015468042.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_010816061.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816061.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814363.1
    CDS991..5928
    Translation

    Target ORF information:

    RefSeq Version XM_010816061.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816061.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_010816062.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816062.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814364.1
    CDS531..5468
    Translation

    Target ORF information:

    RefSeq Version XM_010816062.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816062.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_010816063.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816063.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814365.1
    CDS412..5349
    Translation

    Target ORF information:

    RefSeq Version XM_010816063.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816063.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_010816060.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814362.1
    CDS490..5427
    Translation

    Target ORF information:

    RefSeq Version XM_010816060.2
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816060.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015468034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323520.1
    CDS535..5472
    Translation

    Target ORF information:

    RefSeq Version XM_015468034.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015468037.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323523.1
    CDS991..5928
    Translation

    Target ORF information:

    RefSeq Version XM_015468037.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468037.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015468039.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323525.1
    CDS412..5349
    Translation

    Target ORF information:

    RefSeq Version XM_015468039.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015468036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323522.1
    CDS461..5398
    Translation

    Target ORF information:

    RefSeq Version XM_015468036.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015468038.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323524.1
    CDS531..5468
    Translation

    Target ORF information:

    RefSeq Version XM_015468038.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015468035.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323521.1
    CDS490..5427
    Translation

    Target ORF information:

    RefSeq Version XM_015468035.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_010816059.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814361.1
    CDS442..5379
    Translation

    Target ORF information:

    RefSeq Version XM_010816059.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816059.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_003587412.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587412.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587460.2
    CDS535..5472
    Translation

    Target ORF information:

    RefSeq Version XM_003587412.4
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587412.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015468040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323526.1
    CDS416..5353
    Translation

    Target ORF information:

    RefSeq Version XM_015468040.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29804
    Clone ID Related Accession (Same CDS sequence) XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
    Accession Version XM_015468041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323527.1
    CDS1359..6296
    Translation

    Target ORF information:

    RefSeq Version XM_015468041.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA
    GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG
    CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC
    ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG
    TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG
    GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG
    GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG
    CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC
    GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT
    AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA
    CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC
    TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC
    TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG
    AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC
    GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC
    CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG
    TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC
    GCCAGCACGT CGCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93888
    Clone ID Related Accession (Same CDS sequence) XM_015468043.1 , XM_015458748.1
    Accession Version XM_015468043.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323529.1
    CDS535..5469
    Translation

    Target ORF information:

    RefSeq Version XM_015468043.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468043.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93888
    Clone ID Related Accession (Same CDS sequence) XM_015468043.1 , XM_015458748.1
    Accession Version XM_015458748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 6B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314234.1
    CDS535..5469
    Translation

    Target ORF information:

    RefSeq Version XM_015458748.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
    GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA
    TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA
    CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA
    CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG
    CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG
    CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG
    GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT
    GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA
    CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC
    GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG
    GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT
    CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA
    CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG
    CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA
    CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT
    GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC
    CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT
    GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA
    ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC
    AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT
    GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG
    GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG
    CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC
    CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA
    GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC
    CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC
    ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC
    AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC
    CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT
    CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG
    CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG
    CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG
    CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG
    GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT
    GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC
    CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC
    CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA
    GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG
    TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG
    GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG
    ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT
    CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC
    CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG
    GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA
    CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT
    TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC
    ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG
    GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG
    CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC
    CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG
    GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT
    GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC
    TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT
    TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG
    CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC
    ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT
    GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC
    TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG
    GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG
    CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG
    GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT
    GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT
    CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC
    AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT
    GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG TGCACCAGAC
    CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG
    TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG
    GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC
    ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC
    AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG
    ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA
    ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA
    GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC
    AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA
    TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG
    AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC
    CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG
    AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC
    GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG
    GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG
    GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC
    AGCACGTCGC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.