KDM6B gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014113542.2	XP_013969017.1	lysine-specific demethylase 6B isoform X1
XM_014113545.2	XP_013969020.1	lysine-specific demethylase 6B isoform X2
XM_005619999.3	XP_005620056.1	lysine-specific demethylase 6B isoform X3
XM_014113542.1	XP_013969017.1	lysine-specific demethylase 6B isoform X1
XM_014113544.1	XP_013969019.1	lysine-specific demethylase 6B isoform X1
XM_005619995.2	XP_005620052.1	lysine-specific demethylase 6B isoform X1
XM_005619996.2	XP_005620053.1	lysine-specific demethylase 6B isoform X1
XM_005619992.2	XP_005620049.1	lysine-specific demethylase 6B isoform X1
XM_005619992.3	XP_005620049.1	lysine-specific demethylase 6B isoform X1
XM_014113541.2	XP_013969016.1	lysine-specific demethylase 6B isoform X1
XM_014113544.2	XP_013969019.1	lysine-specific demethylase 6B isoform X1
XM_014113543.2	XP_013969018.1	lysine-specific demethylase 6B isoform X1
XM_022418533.1	XP_022274241.1	lysine-specific demethylase 6B isoform X1
XM_005619998.3	XP_005620055.1	lysine-specific demethylase 6B isoform X1
XM_014113541.1	XP_013969016.1	lysine-specific demethylase 6B isoform X1
XM_005619994.1	XP_005620051.1	lysine-specific demethylase 6B isoform X1
XM_014113545.1	XP_013969020.1	lysine-specific demethylase 6B isoform X2
XM_005619997.2	XP_005620054.1	lysine-specific demethylase 6B isoform X1
XM_014113543.1	XP_013969018.1	lysine-specific demethylase 6B isoform X1
XM_005619998.2	XP_005620055.1	lysine-specific demethylase 6B isoform X1
XM_005619999.2	XP_005620056.1	lysine-specific demethylase 6B isoform X3
XM_022418531.1	XP_022274239.1	lysine-specific demethylase 6B isoform X1
XM_005619995.3	XP_005620052.1	lysine-specific demethylase 6B isoform X1
XM_005619997.3	XP_005620054.1	lysine-specific demethylase 6B isoform X1
XM_005619996.3	XP_005620053.1	lysine-specific demethylase 6B isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe06254	XM_005619997.3 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_014113544.2 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_005619996.3 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_022418531.1 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_014113543.2 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_005619995.3 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_005619994.1 Latest version!	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_005619992.3 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_014113541.2 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_005619998.3 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_014113542.2 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_022418533.1 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06255	XM_005619999.3 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe36421	XM_014113545.2 Latest version!	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OCe06254	XM_014113542.1	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_005619992.2	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_014113544.1	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_014113541.1	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe36421	XM_014113545.1	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_014113543.1	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_005619998.2	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06255	XM_005619999.2	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_005619995.2	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_005619997.2	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe06254	XM_005619996.2	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

KDM6B ( XM_014113542.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969017.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_014113542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969017.1
CDS	411..5372
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_014113542.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113542.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_014113542.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969017.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_014113542.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969017.1
CDS	418..5379
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_014113542.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113542.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_014113544.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969019.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_014113544.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969019.1
CDS	736..5697
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_014113544.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113544.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619995.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620052.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619995.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620052.1
CDS	606..5567
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619995.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619995.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619996.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620053.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619996.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620053.1
CDS	334..5295
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619996.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619996.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619992.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620049.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619992.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620049.1
CDS	526..5487
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619992.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619992.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619992.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620049.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X1, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620049.1
CDS	520..5481
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619992.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619992.3

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_014113541.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969016.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X2, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_014113541.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969016.1
CDS	537..5498
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_014113541.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113541.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_014113544.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969019.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X6, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_014113544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113544.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969019.1
CDS	636..5597
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_014113544.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113544.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_014113543.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969018.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X7, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_014113543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969018.1
CDS	398..5359
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_014113543.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113543.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_022418533.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274241.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_022418533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274241.1
CDS	543..5504
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_022418533.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418533.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619998.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620055.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X10, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619998.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619998.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620055.1
CDS	476..5437
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619998.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619998.3

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_014113541.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969016.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_014113541.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969016.1
CDS	529..5490
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_014113541.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113541.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619994.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620051.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620051.1
CDS	624..5585
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619994.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619994.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619997.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620054.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619997.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620054.1
CDS	602..5563
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619997.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619997.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_014113543.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969018.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_014113543.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969018.1
CDS	322..5283
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_014113543.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113543.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619998.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620055.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619998.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620055.1
CDS	545..5506
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619998.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619998.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_022418531.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022274239.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X3, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_022418531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022274239.1
CDS	407..5368
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_022418531.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418531.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619995.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620052.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X4, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619995.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619995.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620052.1
CDS	451..5412
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619995.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619995.3

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619997.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620054.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X8, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620054.1
CDS	533..5494
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619997.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619997.3

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619996.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620053.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X9, mRNA.

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CloneID	OCe06254
Clone ID Related Accession (Same CDS sequence)	XM_014113542.2 , XM_014113542.1 , XM_014113544.1 , XM_005619995.2 , XM_005619996.2 , XM_005619992.2 , XM_005619992.3 , XM_014113541.2 , XM_014113544.2 , XM_014113543.2 , XM_022418533.1 , XM_005619998.3 , XM_014113541.1 , XM_005619994.1 , XM_005619997.2 , XM_014113543.1 , XM_005619998.2 , XM_022418531.1 , XM_005619995.3 , XM_005619997.3 , XM_005619996.3
Accession Version	XM_005619996.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619996.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620053.1
CDS	367..5328
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619996.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619996.3

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGCAGTGC ACCGGACCCG AAGAACCATC ACATCATCAA GTTTGGCACC 
AACATCGACC TATCCGATGC CAAGAGGTGG AAGCCCCAGC TGCAGGAGCT GCTCAAGCTG 
CCCGCTTTCA TGCGGGTCAC ATCCACTGGC AACATGCTGA GCCACGTGGG CCACACCATC 
CTGGGCATGA ACACCGTGCA GCTGTACATG AAGGTCCCTG GCAGTCGAAC TCCAGGCCAC 
CAGGAGAACA ACAACTTCTG CTCCGTCAAC ATCAACATCG GGCCAGGCGA CTGCGAGTGG 
TTCGCGGTGC ACGAACACTA CTGGGAGACC ATCAGCGCCT TCTGCGACCG GCACGGCGTG 
GACTACCTGA CCGGTTCCTG GTGGCCAATC CTGGATGACC TCTATGCTTC CAACATCCCT 
GTGTACCGCT TCGTGCAGCG CCCCGGAGAC CTCGTGTGGA TTAATGCGGG CACCGTGCAC 
TGGGTGCAGG CCACTGGCTG GTGCAACAAC ATCGCCTGGA ACGTGGGGCC CCTCACCGCC 
TATCAGTACC AGCTGGCCCT GGAACGATAC GAGTGGAACG AGGTGAAGAA CGTCAAATCC 
ATCGTGCCCA TGATTCACGT ATCCTGGAAT GTGGCTCGTA CGGTCAAAAT CAGCGACCCG 
GACTTGTTCA GGATGATCAA GTTCTGCCTC CTGCAGTCCA TGAAGCACTG CCAGGTGCAA 
CGCGAGAGCC TGGTGCGGGC CGGGAAGAAG ATCGCCTACC AGGGCCGGGT CAAGGACGAG 
CCCGCCTACT ACTGCAACGA GTGCGACGTG GAGGTGTTCA ACATCCTGTT TGTGACGAGC 
GAGAACGGCA GCCGCAACAC GTACCTCGTG CACTGCGAGG CCTGTGCGCG GCGCCGCAGT 
GCGGGCCTAC AGGGCGTGGT GGTGCTGGAG CAGTACCGCA CCGAGGAGCT GGCGCAGGCC 
TACGACGCCT TCACGCTGGC CCCCGCCAGC ACGTCGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619999.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620056.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X13, mRNA.

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CloneID	OCe06255
Clone ID Related Accession (Same CDS sequence)	XM_005619999.3 , XM_005619999.2
Accession Version	XM_005619999.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT GCATGGAGAC GTCTGGGGCC CTGACCGCAA GGGTTCGGTG 
CCCCCAGAAC GCCAGGAGCA GCGGCACCCG CTGCCCCACC CGTATCCATA CCCGGTTCCG 
GCCTATACCA CGCACCCCCC TGGCCACCGG CTGGTCCCGG CCGCACCCCC AGGCCCCGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCACCACC 
AGCAGCAGCA GTAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAACACCGG TCTCCGGGGC 
GCGGAGCCGA ACCCAGGCAT CCCCGGCGCT GACCATTACC AAACTCCCGC GCTGGACATC 
TCCTCCCACC AAGGCCGCCT GGGGCCCTCG GCGCACAGCA GTCGGAAACC GTTCCTGGCG 
GCTCCCGCTG CCACTCCTCA CCTGTCCCTG CCACCTGGAC CCCCGTCACC TCCTCCACCA 
CCCTGTCCCC GCCTCCTACG CCCCCCACCA CCTCCTGCCT GGCTGAAGGG CCCGGCTTGC 
CGGGCAGCCC GCGAGGATGG GGAGATCTTA GAGGAGCTCT TCTTTGGGGC TGAGGGACGC 
CCCCGCCCTC CCCCACCACC CCTCCTTCAC CGCGAGGGCT TCTTGGGGCC TCCGGCTCCC 
CGCTTTTCTG TGGGCACTCA GGATTCTCAC ACCCCTCCCA CCCCCCCAGC CACCAGCAGC 
AGCAGCAGCA ACCATGGCAG CCACAGCAGC AGCCCTACGG GGCCTGTGTC CTTCCCTCCG 
CCTCCCTATC TGGCCAGAAG TTTGGACCCC CTTCCGCGGC CCCCCAGCCC CACACTGAGC 
CCCCAGGACC CACCTCTTGC ACCCTTGACT CTTGCCCTGC CTCCAGCTCC TCCCTCCTCC 
TGCCACCAAA ATACCTCAGG AAGCTTCAGG CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC 
TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG CCATCCCCAG GCCCACTGAA TAAAGCCCCC 
CAGCCTGTGC CGCCCAGGGT TGGGGAGCTG CCTGCCCGAG GCCCACGGCT CTTCGATTTT 
CCCCCTACCC CACTGGAGGA CCAGTTTGAG GAGCCGGCTG AATTCAAGAT CCTCCCTGAC 
GGGCTGGCCA ACATCATGAA GATGCTGGAC GAATCCATTC GCAAGGAGGA GGAGCAGCAG 
CAACAGGAGG CAGGCGTGGG CCCCCCGCCC CCGCTGAAGG AGCCCTTTCC ATCTCTGCAG 
CCTTCATTCC CCAGTGACAC AGCCCCAGCC ACCACGGCAG CCCCGGCAGC CACCGCTACC 
ACCACCACTG CCACCCAGGA AGAAGAGAAG AAGCCACCAC CAGTCCTGCC ACCGCCGCCG 
CCTCTTGCGA AGTTCCCACC GCCCCAGCCC CAGCCCCCAC CGCCGCCCCC GCTACCCCCA 
GCAGCCAGCC CAGCCAGCCT GCTCAAATCC TTGGCCTCCG TGCTGGAGGG ACAGAAGTAC 
TGTTACCGGG GGACCGGAGC AGCTGTTGCC ACCCGGCCCG GGCCCTTGCC CACCACGCAG 
TATTCCCCCG GCCCCCCGTC AGGTGCTACC GCCCCGCCGC CTGCCTCGGC GGCCCCCAGC 
GCCCGGGGCT CCCCGCAGCC CTCTGCTTCC TCGTCATCTC AGTTCTCTAC CTCAGGCGGG 
CCCTGGGCCC GGGAGCGCAG GGCGGGCGAA GAGCCAGCCC GAGGCCCCAC AGCCCCTGCC 
CCACCGCCGC CACCGCCCCC ACCCCTGCCT CTGCCTCCTG CTCGATCTGA GTCTGAGGTG 
CTAGAAGAGA TCAGTCGGGC TTGTGAGACC CTCGTGGAAC GGGTGGGCCG GAGCGCCGCA 
GACCTGGCCG GCCCGGTGGA CACGGCAGGC CCGGTGGACA GTGGGACAGA GCGGCTGTTG 
CCTCCTGCCC AGGCCCGGGA GGAGGGTGGC GGGGCAGTGG CAGCAGCAGG ACCTGGCAGT 
GGCAAGCGGC GGCAGAAGGA GCATCAGAAG GAGCACCAGA AGGAGCATCG GCGGCACCGG 
CGAGCTTGTA AGGACAGCAC GGGTCGGCGG CCCCGAGAGG GCAGAGTGAA GACCAAGGCC 
AAGGCCCCCA AAGAAAAGAG CCGCCGGGTG CTGGGCAACC TGGACCTGCA GAGTGAGGAG 
ATCCAGGGGC GTGAGAAGGC TCGGCCCGAT CTCGGCGGGG CCTCCAAGGC CAAGCCACCC 
GCAGCCCCGG CCCCTCCACC AGCTCCTGCA CCTGCTGCCC AGCCCACACC GCCCTCCGCT 
CCCACACCAG GGAAGAAGGC CCGGGAGGAA GCGCCGGGGC CCCCTGGGGT CAGCCGGGCT 
GACATGCTGA AGCTGCGCTC GCTTAGTGAG GGGCCCCCCA AGGAGCTGAA GATCCGCCTC 
ATCAAGGTGG AGAGCGGTGA CAAGGAGACC TTTATTGCCT CTGAGGTGGA AGAGCGGAGG 
CTGCGCATGG CGGACCTTAC CATCAGCCAC TGCGCTGCCG ACGTCGTGCG GGCCAGCAAG 
AACGCCAAGG TGAAAGGGAA GTTTCGAGAG TCCTACCTTT CCCCCGCCCA GTCTGTGAAA 
CCCAAGATCA ACACTGAGGA GAAGCTACCC CGGGAAAAAC TCAACCCACC CACCCCCAGC 
ATCTATCTGG AGAGCAAACG GGACGCCTTC TCACCCGTGC TACTGCAGTT CTGTACGGAC 
CCTCGAAATC CCATCACCGT GATCCGGGGC CTGGCGGGCT CCCTGCGGCT CAACTTGGGC 
CTCTTCTCCA CCAAGACGCT GGTGGAGGCA AGCGGCGAGC ACACGGTGGA GGTGCGCACG 
CAGGTGCAGC AGCCCTCCGA TGAGAACTGG GATCTGACGG GCACGCGGCA GATCTGGCCC 
TGCGAGAGCT CCCGTTCCCA TACCACCATT GCCAAGTATG CGCAGTACCA GGCCTCATCC 
TTTCAGGAGT CCCTGCAGGA GGAGAAGGAG AGTGAGGATG AGGAATCTGA GGAGCCCGAC 
AGCACCACAG GAACCCCTCC CAGCAGTGCA CCGGACCCGA AGAACCATCA CATCATCAAG 
TTTGGCACCA ACATCGACCT ATCCGATGCC AAGAGGTGGA AGCCCCAGCT GCAGGAGCTG 
CTCAAGCTGC CCGCTTTCAT GCGGGTCACA TCCACTGGCA ACATGCTGAG CCACGTGGGC 
CACACCATCC TGGGCATGAA CACCGTGCAG CTGTACATGA AGGTCCCTGG CAGTCGAACT 
CCAGGCCACC AGGAGAACAA CAACTTCTGC TCCGTCAACA TCAACATCGG GCCAGGCGAC 
TGCGAGTGGT TCGCGGTGCA CGAACACTAC TGGGAGACCA TCAGCGCCTT CTGCGACCGG 
CACGGCGTGG ACTACCTGAC CGGTTCCTGG TGGCCAATCC TGGATGACCT CTATGCTTCC 
AACATCCCTG TGTACCGCTT CGTGCAGCGC CCCGGAGACC TCGTGTGGAT TAATGCGGGC 
ACCGTGCACT GGGTGCAGGC CACTGGCTGG TGCAACAACA TCGCCTGGAA CGTGGGGCCC 
CTCACCGCCT ATCAGTACCA GCTGGCCCTG GAACGATACG AGTGGAACGA GGTGAAGAAC 
GTCAAATCCA TCGTGCCCAT GATTCACGTA TCCTGGAATG TGGCTCGTAC GGTCAAAATC 
AGCGACCCGG ACTTGTTCAG GATGATCAAG TTCTGCCTCC TGCAGTCCAT GAAGCACTGC 
CAGGTGCAAC GCGAGAGCCT GGTGCGGGCC GGGAAGAAGA TCGCCTACCA GGGCCGGGTC 
AAGGACGAGC CCGCCTACTA CTGCAACGAG TGCGACGTGG AGGTGTTCAA CATCCTGTTT 
GTGACGAGCG AGAACGGCAG CCGCAACACG TACCTCGTGC ACTGCGAGGC CTGTGCGCGG 
CGCCGCAGTG CGGGCCTACA GGGCGTGGTG GTGCTGGAGC AGTACCGCAC CGAGGAGCTG 
GCGCAGGCCT ACGACGCCTT CACGCTGGCC CCCGCCAGCA CGTCGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620056.1
CDS	520..5370
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619999.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619999.3

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT GCATGGAGAC GTCTGGGGCC CTGACCGCAA GGGTTCGGTG 
CCCCCAGAAC GCCAGGAGCA GCGGCACCCG CTGCCCCACC CGTATCCATA CCCGGTTCCG 
GCCTATACCA CGCACCCCCC TGGCCACCGG CTGGTCCCGG CCGCACCCCC AGGCCCCGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCACCACC 
AGCAGCAGCA GTAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAACACCGG TCTCCGGGGC 
GCGGAGCCGA ACCCAGGCAT CCCCGGCGCT GACCATTACC AAACTCCCGC GCTGGACATC 
TCCTCCCACC AAGGCCGCCT GGGGCCCTCG GCGCACAGCA GTCGGAAACC GTTCCTGGCG 
GCTCCCGCTG CCACTCCTCA CCTGTCCCTG CCACCTGGAC CCCCGTCACC TCCTCCACCA 
CCCTGTCCCC GCCTCCTACG CCCCCCACCA CCTCCTGCCT GGCTGAAGGG CCCGGCTTGC 
CGGGCAGCCC GCGAGGATGG GGAGATCTTA GAGGAGCTCT TCTTTGGGGC TGAGGGACGC 
CCCCGCCCTC CCCCACCACC CCTCCTTCAC CGCGAGGGCT TCTTGGGGCC TCCGGCTCCC 
CGCTTTTCTG TGGGCACTCA GGATTCTCAC ACCCCTCCCA CCCCCCCAGC CACCAGCAGC 
AGCAGCAGCA ACCATGGCAG CCACAGCAGC AGCCCTACGG GGCCTGTGTC CTTCCCTCCG 
CCTCCCTATC TGGCCAGAAG TTTGGACCCC CTTCCGCGGC CCCCCAGCCC CACACTGAGC 
CCCCAGGACC CACCTCTTGC ACCCTTGACT CTTGCCCTGC CTCCAGCTCC TCCCTCCTCC 
TGCCACCAAA ATACCTCAGG AAGCTTCAGG CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC 
TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG CCATCCCCAG GCCCACTGAA TAAAGCCCCC 
CAGCCTGTGC CGCCCAGGGT TGGGGAGCTG CCTGCCCGAG GCCCACGGCT CTTCGATTTT 
CCCCCTACCC CACTGGAGGA CCAGTTTGAG GAGCCGGCTG AATTCAAGAT CCTCCCTGAC 
GGGCTGGCCA ACATCATGAA GATGCTGGAC GAATCCATTC GCAAGGAGGA GGAGCAGCAG 
CAACAGGAGG CAGGCGTGGG CCCCCCGCCC CCGCTGAAGG AGCCCTTTCC ATCTCTGCAG 
CCTTCATTCC CCAGTGACAC AGCCCCAGCC ACCACGGCAG CCCCGGCAGC CACCGCTACC 
ACCACCACTG CCACCCAGGA AGAAGAGAAG AAGCCACCAC CAGTCCTGCC ACCGCCGCCG 
CCTCTTGCGA AGTTCCCACC GCCCCAGCCC CAGCCCCCAC CGCCGCCCCC GCTACCCCCA 
GCAGCCAGCC CAGCCAGCCT GCTCAAATCC TTGGCCTCCG TGCTGGAGGG ACAGAAGTAC 
TGTTACCGGG GGACCGGAGC AGCTGTTGCC ACCCGGCCCG GGCCCTTGCC CACCACGCAG 
TATTCCCCCG GCCCCCCGTC AGGTGCTACC GCCCCGCCGC CTGCCTCGGC GGCCCCCAGC 
GCCCGGGGCT CCCCGCAGCC CTCTGCTTCC TCGTCATCTC AGTTCTCTAC CTCAGGCGGG 
CCCTGGGCCC GGGAGCGCAG GGCGGGCGAA GAGCCAGCCC GAGGCCCCAC AGCCCCTGCC 
CCACCGCCGC CACCGCCCCC ACCCCTGCCT CTGCCTCCTG CTCGATCTGA GTCTGAGGTG 
CTAGAAGAGA TCAGTCGGGC TTGTGAGACC CTCGTGGAAC GGGTGGGCCG GAGCGCCGCA 
GACCTGGCCG GCCCGGTGGA CACGGCAGGC CCGGTGGACA GTGGGACAGA GCGGCTGTTG 
CCTCCTGCCC AGGCCCGGGA GGAGGGTGGC GGGGCAGTGG CAGCAGCAGG ACCTGGCAGT 
GGCAAGCGGC GGCAGAAGGA GCATCAGAAG GAGCACCAGA AGGAGCATCG GCGGCACCGG 
CGAGCTTGTA AGGACAGCAC GGGTCGGCGG CCCCGAGAGG GCAGAGTGAA GACCAAGGCC 
AAGGCCCCCA AAGAAAAGAG CCGCCGGGTG CTGGGCAACC TGGACCTGCA GAGTGAGGAG 
ATCCAGGGGC GTGAGAAGGC TCGGCCCGAT CTCGGCGGGG CCTCCAAGGC CAAGCCACCC 
GCAGCCCCGG CCCCTCCACC AGCTCCTGCA CCTGCTGCCC AGCCCACACC GCCCTCCGCT 
CCCACACCAG GGAAGAAGGC CCGGGAGGAA GCGCCGGGGC CCCCTGGGGT CAGCCGGGCT 
GACATGCTGA AGCTGCGCTC GCTTAGTGAG GGGCCCCCCA AGGAGCTGAA GATCCGCCTC 
ATCAAGGTGG AGAGCGGTGA CAAGGAGACC TTTATTGCCT CTGAGGTGGA AGAGCGGAGG 
CTGCGCATGG CGGACCTTAC CATCAGCCAC TGCGCTGCCG ACGTCGTGCG GGCCAGCAAG 
AACGCCAAGG TGAAAGGGAA GTTTCGAGAG TCCTACCTTT CCCCCGCCCA GTCTGTGAAA 
CCCAAGATCA ACACTGAGGA GAAGCTACCC CGGGAAAAAC TCAACCCACC CACCCCCAGC 
ATCTATCTGG AGAGCAAACG GGACGCCTTC TCACCCGTGC TACTGCAGTT CTGTACGGAC 
CCTCGAAATC CCATCACCGT GATCCGGGGC CTGGCGGGCT CCCTGCGGCT CAACTTGGGC 
CTCTTCTCCA CCAAGACGCT GGTGGAGGCA AGCGGCGAGC ACACGGTGGA GGTGCGCACG 
CAGGTGCAGC AGCCCTCCGA TGAGAACTGG GATCTGACGG GCACGCGGCA GATCTGGCCC 
TGCGAGAGCT CCCGTTCCCA TACCACCATT GCCAAGTATG CGCAGTACCA GGCCTCATCC 
TTTCAGGAGT CCCTGCAGGA GGAGAAGGAG AGTGAGGATG AGGAATCTGA GGAGCCCGAC 
AGCACCACAG GAACCCCTCC CAGCAGTGCA CCGGACCCGA AGAACCATCA CATCATCAAG 
TTTGGCACCA ACATCGACCT ATCCGATGCC AAGAGGTGGA AGCCCCAGCT GCAGGAGCTG 
CTCAAGCTGC CCGCTTTCAT GCGGGTCACA TCCACTGGCA ACATGCTGAG CCACGTGGGC 
CACACCATCC TGGGCATGAA CACCGTGCAG CTGTACATGA AGGTCCCTGG CAGTCGAACT 
CCAGGCCACC AGGAGAACAA CAACTTCTGC TCCGTCAACA TCAACATCGG GCCAGGCGAC 
TGCGAGTGGT TCGCGGTGCA CGAACACTAC TGGGAGACCA TCAGCGCCTT CTGCGACCGG 
CACGGCGTGG ACTACCTGAC CGGTTCCTGG TGGCCAATCC TGGATGACCT CTATGCTTCC 
AACATCCCTG TGTACCGCTT CGTGCAGCGC CCCGGAGACC TCGTGTGGAT TAATGCGGGC 
ACCGTGCACT GGGTGCAGGC CACTGGCTGG TGCAACAACA TCGCCTGGAA CGTGGGGCCC 
CTCACCGCCT ATCAGTACCA GCTGGCCCTG GAACGATACG AGTGGAACGA GGTGAAGAAC 
GTCAAATCCA TCGTGCCCAT GATTCACGTA TCCTGGAATG TGGCTCGTAC GGTCAAAATC 
AGCGACCCGG ACTTGTTCAG GATGATCAAG TTCTGCCTCC TGCAGTCCAT GAAGCACTGC 
CAGGTGCAAC GCGAGAGCCT GGTGCGGGCC GGGAAGAAGA TCGCCTACCA GGGCCGGGTC 
AAGGACGAGC CCGCCTACTA CTGCAACGAG TGCGACGTGG AGGTGTTCAA CATCCTGTTT 
GTGACGAGCG AGAACGGCAG CCGCAACACG TACCTCGTGC ACTGCGAGGC CTGTGCGCGG 
CGCCGCAGTG CGGGCCTACA GGGCGTGGTG GTGCTGGAGC AGTACCGCAC CGAGGAGCTG 
GCGCAGGCCT ACGACGCCTT CACGCTGGCC CCCGCCAGCA CGTCGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_005619999.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005620056.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X12, mRNA.

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CloneID	OCe06255
Clone ID Related Accession (Same CDS sequence)	XM_005619999.3 , XM_005619999.2
Accession Version	XM_005619999.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT GCATGGAGAC GTCTGGGGCC CTGACCGCAA GGGTTCGGTG 
CCCCCAGAAC GCCAGGAGCA GCGGCACCCG CTGCCCCACC CGTATCCATA CCCGGTTCCG 
GCCTATACCA CGCACCCCCC TGGCCACCGG CTGGTCCCGG CCGCACCCCC AGGCCCCGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCACCACC 
AGCAGCAGCA GTAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAACACCGG TCTCCGGGGC 
GCGGAGCCGA ACCCAGGCAT CCCCGGCGCT GACCATTACC AAACTCCCGC GCTGGACATC 
TCCTCCCACC AAGGCCGCCT GGGGCCCTCG GCGCACAGCA GTCGGAAACC GTTCCTGGCG 
GCTCCCGCTG CCACTCCTCA CCTGTCCCTG CCACCTGGAC CCCCGTCACC TCCTCCACCA 
CCCTGTCCCC GCCTCCTACG CCCCCCACCA CCTCCTGCCT GGCTGAAGGG CCCGGCTTGC 
CGGGCAGCCC GCGAGGATGG GGAGATCTTA GAGGAGCTCT TCTTTGGGGC TGAGGGACGC 
CCCCGCCCTC CCCCACCACC CCTCCTTCAC CGCGAGGGCT TCTTGGGGCC TCCGGCTCCC 
CGCTTTTCTG TGGGCACTCA GGATTCTCAC ACCCCTCCCA CCCCCCCAGC CACCAGCAGC 
AGCAGCAGCA ACCATGGCAG CCACAGCAGC AGCCCTACGG GGCCTGTGTC CTTCCCTCCG 
CCTCCCTATC TGGCCAGAAG TTTGGACCCC CTTCCGCGGC CCCCCAGCCC CACACTGAGC 
CCCCAGGACC CACCTCTTGC ACCCTTGACT CTTGCCCTGC CTCCAGCTCC TCCCTCCTCC 
TGCCACCAAA ATACCTCAGG AAGCTTCAGG CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC 
TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG CCATCCCCAG GCCCACTGAA TAAAGCCCCC 
CAGCCTGTGC CGCCCAGGGT TGGGGAGCTG CCTGCCCGAG GCCCACGGCT CTTCGATTTT 
CCCCCTACCC CACTGGAGGA CCAGTTTGAG GAGCCGGCTG AATTCAAGAT CCTCCCTGAC 
GGGCTGGCCA ACATCATGAA GATGCTGGAC GAATCCATTC GCAAGGAGGA GGAGCAGCAG 
CAACAGGAGG CAGGCGTGGG CCCCCCGCCC CCGCTGAAGG AGCCCTTTCC ATCTCTGCAG 
CCTTCATTCC CCAGTGACAC AGCCCCAGCC ACCACGGCAG CCCCGGCAGC CACCGCTACC 
ACCACCACTG CCACCCAGGA AGAAGAGAAG AAGCCACCAC CAGTCCTGCC ACCGCCGCCG 
CCTCTTGCGA AGTTCCCACC GCCCCAGCCC CAGCCCCCAC CGCCGCCCCC GCTACCCCCA 
GCAGCCAGCC CAGCCAGCCT GCTCAAATCC TTGGCCTCCG TGCTGGAGGG ACAGAAGTAC 
TGTTACCGGG GGACCGGAGC AGCTGTTGCC ACCCGGCCCG GGCCCTTGCC CACCACGCAG 
TATTCCCCCG GCCCCCCGTC AGGTGCTACC GCCCCGCCGC CTGCCTCGGC GGCCCCCAGC 
GCCCGGGGCT CCCCGCAGCC CTCTGCTTCC TCGTCATCTC AGTTCTCTAC CTCAGGCGGG 
CCCTGGGCCC GGGAGCGCAG GGCGGGCGAA GAGCCAGCCC GAGGCCCCAC AGCCCCTGCC 
CCACCGCCGC CACCGCCCCC ACCCCTGCCT CTGCCTCCTG CTCGATCTGA GTCTGAGGTG 
CTAGAAGAGA TCAGTCGGGC TTGTGAGACC CTCGTGGAAC GGGTGGGCCG GAGCGCCGCA 
GACCTGGCCG GCCCGGTGGA CACGGCAGGC CCGGTGGACA GTGGGACAGA GCGGCTGTTG 
CCTCCTGCCC AGGCCCGGGA GGAGGGTGGC GGGGCAGTGG CAGCAGCAGG ACCTGGCAGT 
GGCAAGCGGC GGCAGAAGGA GCATCAGAAG GAGCACCAGA AGGAGCATCG GCGGCACCGG 
CGAGCTTGTA AGGACAGCAC GGGTCGGCGG CCCCGAGAGG GCAGAGTGAA GACCAAGGCC 
AAGGCCCCCA AAGAAAAGAG CCGCCGGGTG CTGGGCAACC TGGACCTGCA GAGTGAGGAG 
ATCCAGGGGC GTGAGAAGGC TCGGCCCGAT CTCGGCGGGG CCTCCAAGGC CAAGCCACCC 
GCAGCCCCGG CCCCTCCACC AGCTCCTGCA CCTGCTGCCC AGCCCACACC GCCCTCCGCT 
CCCACACCAG GGAAGAAGGC CCGGGAGGAA GCGCCGGGGC CCCCTGGGGT CAGCCGGGCT 
GACATGCTGA AGCTGCGCTC GCTTAGTGAG GGGCCCCCCA AGGAGCTGAA GATCCGCCTC 
ATCAAGGTGG AGAGCGGTGA CAAGGAGACC TTTATTGCCT CTGAGGTGGA AGAGCGGAGG 
CTGCGCATGG CGGACCTTAC CATCAGCCAC TGCGCTGCCG ACGTCGTGCG GGCCAGCAAG 
AACGCCAAGG TGAAAGGGAA GTTTCGAGAG TCCTACCTTT CCCCCGCCCA GTCTGTGAAA 
CCCAAGATCA ACACTGAGGA GAAGCTACCC CGGGAAAAAC TCAACCCACC CACCCCCAGC 
ATCTATCTGG AGAGCAAACG GGACGCCTTC TCACCCGTGC TACTGCAGTT CTGTACGGAC 
CCTCGAAATC CCATCACCGT GATCCGGGGC CTGGCGGGCT CCCTGCGGCT CAACTTGGGC 
CTCTTCTCCA CCAAGACGCT GGTGGAGGCA AGCGGCGAGC ACACGGTGGA GGTGCGCACG 
CAGGTGCAGC AGCCCTCCGA TGAGAACTGG GATCTGACGG GCACGCGGCA GATCTGGCCC 
TGCGAGAGCT CCCGTTCCCA TACCACCATT GCCAAGTATG CGCAGTACCA GGCCTCATCC 
TTTCAGGAGT CCCTGCAGGA GGAGAAGGAG AGTGAGGATG AGGAATCTGA GGAGCCCGAC 
AGCACCACAG GAACCCCTCC CAGCAGTGCA CCGGACCCGA AGAACCATCA CATCATCAAG 
TTTGGCACCA ACATCGACCT ATCCGATGCC AAGAGGTGGA AGCCCCAGCT GCAGGAGCTG 
CTCAAGCTGC CCGCTTTCAT GCGGGTCACA TCCACTGGCA ACATGCTGAG CCACGTGGGC 
CACACCATCC TGGGCATGAA CACCGTGCAG CTGTACATGA AGGTCCCTGG CAGTCGAACT 
CCAGGCCACC AGGAGAACAA CAACTTCTGC TCCGTCAACA TCAACATCGG GCCAGGCGAC 
TGCGAGTGGT TCGCGGTGCA CGAACACTAC TGGGAGACCA TCAGCGCCTT CTGCGACCGG 
CACGGCGTGG ACTACCTGAC CGGTTCCTGG TGGCCAATCC TGGATGACCT CTATGCTTCC 
AACATCCCTG TGTACCGCTT CGTGCAGCGC CCCGGAGACC TCGTGTGGAT TAATGCGGGC 
ACCGTGCACT GGGTGCAGGC CACTGGCTGG TGCAACAACA TCGCCTGGAA CGTGGGGCCC 
CTCACCGCCT ATCAGTACCA GCTGGCCCTG GAACGATACG AGTGGAACGA GGTGAAGAAC 
GTCAAATCCA TCGTGCCCAT GATTCACGTA TCCTGGAATG TGGCTCGTAC GGTCAAAATC 
AGCGACCCGG ACTTGTTCAG GATGATCAAG TTCTGCCTCC TGCAGTCCAT GAAGCACTGC 
CAGGTGCAAC GCGAGAGCCT GGTGCGGGCC GGGAAGAAGA TCGCCTACCA GGGCCGGGTC 
AAGGACGAGC CCGCCTACTA CTGCAACGAG TGCGACGTGG AGGTGTTCAA CATCCTGTTT 
GTGACGAGCG AGAACGGCAG CCGCAACACG TACCTCGTGC ACTGCGAGGC CTGTGCGCGG 
CGCCGCAGTG CGGGCCTACA GGGCGTGGTG GTGCTGGAGC AGTACCGCAC CGAGGAGCTG 
GCGCAGGCCT ACGACGCCTT CACGCTGGCC CCCGCCAGCA CGTCGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620056.1
CDS	526..5376
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_005619999.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619999.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT GCATGGAGAC GTCTGGGGCC CTGACCGCAA GGGTTCGGTG 
CCCCCAGAAC GCCAGGAGCA GCGGCACCCG CTGCCCCACC CGTATCCATA CCCGGTTCCG 
GCCTATACCA CGCACCCCCC TGGCCACCGG CTGGTCCCGG CCGCACCCCC AGGCCCCGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG GCCTCCCCGG CACCACCACC 
AGCAGCAGCA GTAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAACACCGG TCTCCGGGGC 
GCGGAGCCGA ACCCAGGCAT CCCCGGCGCT GACCATTACC AAACTCCCGC GCTGGACATC 
TCCTCCCACC AAGGCCGCCT GGGGCCCTCG GCGCACAGCA GTCGGAAACC GTTCCTGGCG 
GCTCCCGCTG CCACTCCTCA CCTGTCCCTG CCACCTGGAC CCCCGTCACC TCCTCCACCA 
CCCTGTCCCC GCCTCCTACG CCCCCCACCA CCTCCTGCCT GGCTGAAGGG CCCGGCTTGC 
CGGGCAGCCC GCGAGGATGG GGAGATCTTA GAGGAGCTCT TCTTTGGGGC TGAGGGACGC 
CCCCGCCCTC CCCCACCACC CCTCCTTCAC CGCGAGGGCT TCTTGGGGCC TCCGGCTCCC 
CGCTTTTCTG TGGGCACTCA GGATTCTCAC ACCCCTCCCA CCCCCCCAGC CACCAGCAGC 
AGCAGCAGCA ACCATGGCAG CCACAGCAGC AGCCCTACGG GGCCTGTGTC CTTCCCTCCG 
CCTCCCTATC TGGCCAGAAG TTTGGACCCC CTTCCGCGGC CCCCCAGCCC CACACTGAGC 
CCCCAGGACC CACCTCTTGC ACCCTTGACT CTTGCCCTGC CTCCAGCTCC TCCCTCCTCC 
TGCCACCAAA ATACCTCAGG AAGCTTCAGG CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC 
TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG CCATCCCCAG GCCCACTGAA TAAAGCCCCC 
CAGCCTGTGC CGCCCAGGGT TGGGGAGCTG CCTGCCCGAG GCCCACGGCT CTTCGATTTT 
CCCCCTACCC CACTGGAGGA CCAGTTTGAG GAGCCGGCTG AATTCAAGAT CCTCCCTGAC 
GGGCTGGCCA ACATCATGAA GATGCTGGAC GAATCCATTC GCAAGGAGGA GGAGCAGCAG 
CAACAGGAGG CAGGCGTGGG CCCCCCGCCC CCGCTGAAGG AGCCCTTTCC ATCTCTGCAG 
CCTTCATTCC CCAGTGACAC AGCCCCAGCC ACCACGGCAG CCCCGGCAGC CACCGCTACC 
ACCACCACTG CCACCCAGGA AGAAGAGAAG AAGCCACCAC CAGTCCTGCC ACCGCCGCCG 
CCTCTTGCGA AGTTCCCACC GCCCCAGCCC CAGCCCCCAC CGCCGCCCCC GCTACCCCCA 
GCAGCCAGCC CAGCCAGCCT GCTCAAATCC TTGGCCTCCG TGCTGGAGGG ACAGAAGTAC 
TGTTACCGGG GGACCGGAGC AGCTGTTGCC ACCCGGCCCG GGCCCTTGCC CACCACGCAG 
TATTCCCCCG GCCCCCCGTC AGGTGCTACC GCCCCGCCGC CTGCCTCGGC GGCCCCCAGC 
GCCCGGGGCT CCCCGCAGCC CTCTGCTTCC TCGTCATCTC AGTTCTCTAC CTCAGGCGGG 
CCCTGGGCCC GGGAGCGCAG GGCGGGCGAA GAGCCAGCCC GAGGCCCCAC AGCCCCTGCC 
CCACCGCCGC CACCGCCCCC ACCCCTGCCT CTGCCTCCTG CTCGATCTGA GTCTGAGGTG 
CTAGAAGAGA TCAGTCGGGC TTGTGAGACC CTCGTGGAAC GGGTGGGCCG GAGCGCCGCA 
GACCTGGCCG GCCCGGTGGA CACGGCAGGC CCGGTGGACA GTGGGACAGA GCGGCTGTTG 
CCTCCTGCCC AGGCCCGGGA GGAGGGTGGC GGGGCAGTGG CAGCAGCAGG ACCTGGCAGT 
GGCAAGCGGC GGCAGAAGGA GCATCAGAAG GAGCACCAGA AGGAGCATCG GCGGCACCGG 
CGAGCTTGTA AGGACAGCAC GGGTCGGCGG CCCCGAGAGG GCAGAGTGAA GACCAAGGCC 
AAGGCCCCCA AAGAAAAGAG CCGCCGGGTG CTGGGCAACC TGGACCTGCA GAGTGAGGAG 
ATCCAGGGGC GTGAGAAGGC TCGGCCCGAT CTCGGCGGGG CCTCCAAGGC CAAGCCACCC 
GCAGCCCCGG CCCCTCCACC AGCTCCTGCA CCTGCTGCCC AGCCCACACC GCCCTCCGCT 
CCCACACCAG GGAAGAAGGC CCGGGAGGAA GCGCCGGGGC CCCCTGGGGT CAGCCGGGCT 
GACATGCTGA AGCTGCGCTC GCTTAGTGAG GGGCCCCCCA AGGAGCTGAA GATCCGCCTC 
ATCAAGGTGG AGAGCGGTGA CAAGGAGACC TTTATTGCCT CTGAGGTGGA AGAGCGGAGG 
CTGCGCATGG CGGACCTTAC CATCAGCCAC TGCGCTGCCG ACGTCGTGCG GGCCAGCAAG 
AACGCCAAGG TGAAAGGGAA GTTTCGAGAG TCCTACCTTT CCCCCGCCCA GTCTGTGAAA 
CCCAAGATCA ACACTGAGGA GAAGCTACCC CGGGAAAAAC TCAACCCACC CACCCCCAGC 
ATCTATCTGG AGAGCAAACG GGACGCCTTC TCACCCGTGC TACTGCAGTT CTGTACGGAC 
CCTCGAAATC CCATCACCGT GATCCGGGGC CTGGCGGGCT CCCTGCGGCT CAACTTGGGC 
CTCTTCTCCA CCAAGACGCT GGTGGAGGCA AGCGGCGAGC ACACGGTGGA GGTGCGCACG 
CAGGTGCAGC AGCCCTCCGA TGAGAACTGG GATCTGACGG GCACGCGGCA GATCTGGCCC 
TGCGAGAGCT CCCGTTCCCA TACCACCATT GCCAAGTATG CGCAGTACCA GGCCTCATCC 
TTTCAGGAGT CCCTGCAGGA GGAGAAGGAG AGTGAGGATG AGGAATCTGA GGAGCCCGAC 
AGCACCACAG GAACCCCTCC CAGCAGTGCA CCGGACCCGA AGAACCATCA CATCATCAAG 
TTTGGCACCA ACATCGACCT ATCCGATGCC AAGAGGTGGA AGCCCCAGCT GCAGGAGCTG 
CTCAAGCTGC CCGCTTTCAT GCGGGTCACA TCCACTGGCA ACATGCTGAG CCACGTGGGC 
CACACCATCC TGGGCATGAA CACCGTGCAG CTGTACATGA AGGTCCCTGG CAGTCGAACT 
CCAGGCCACC AGGAGAACAA CAACTTCTGC TCCGTCAACA TCAACATCGG GCCAGGCGAC 
TGCGAGTGGT TCGCGGTGCA CGAACACTAC TGGGAGACCA TCAGCGCCTT CTGCGACCGG 
CACGGCGTGG ACTACCTGAC CGGTTCCTGG TGGCCAATCC TGGATGACCT CTATGCTTCC 
AACATCCCTG TGTACCGCTT CGTGCAGCGC CCCGGAGACC TCGTGTGGAT TAATGCGGGC 
ACCGTGCACT GGGTGCAGGC CACTGGCTGG TGCAACAACA TCGCCTGGAA CGTGGGGCCC 
CTCACCGCCT ATCAGTACCA GCTGGCCCTG GAACGATACG AGTGGAACGA GGTGAAGAAC 
GTCAAATCCA TCGTGCCCAT GATTCACGTA TCCTGGAATG TGGCTCGTAC GGTCAAAATC 
AGCGACCCGG ACTTGTTCAG GATGATCAAG TTCTGCCTCC TGCAGTCCAT GAAGCACTGC 
CAGGTGCAAC GCGAGAGCCT GGTGCGGGCC GGGAAGAAGA TCGCCTACCA GGGCCGGGTC 
AAGGACGAGC CCGCCTACTA CTGCAACGAG TGCGACGTGG AGGTGTTCAA CATCCTGTTT 
GTGACGAGCG AGAACGGCAG CCGCAACACG TACCTCGTGC ACTGCGAGGC CTGTGCGCGG 
CGCCGCAGTG CGGGCCTACA GGGCGTGGTG GTGCTGGAGC AGTACCGCAC CGAGGAGCTG 
GCGCAGGCCT ACGACGCCTT CACGCTGGCC CCCGCCAGCA CGTCGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_014113545.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969020.1 Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe36421
Clone ID Related Accession (Same CDS sequence)	XM_014113545.2 , XM_014113545.1
Accession Version	XM_014113545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysine-specific demethylase 6B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGTGCACC GGACCCGAAG AACCATCACA TCATCAAGTT TGGCACCAAC 
ATCGACCTAT CCGATGCCAA GAGGTGGAAG CCCCAGCTGC AGGAGCTGCT CAAGCTGCCC 
GCTTTCATGC GGGTCACATC CACTGGCAAC ATGCTGAGCC ACGTGGGCCA CACCATCCTG 
GGCATGAACA CCGTGCAGCT GTACATGAAG GTCCCTGGCA GTCGAACTCC AGGCCACCAG 
GAGAACAACA ACTTCTGCTC CGTCAACATC AACATCGGGC CAGGCGACTG CGAGTGGTTC 
GCGGTGCACG AACACTACTG GGAGACCATC AGCGCCTTCT GCGACCGGCA CGGCGTGGAC 
TACCTGACCG GTTCCTGGTG GCCAATCCTG GATGACCTCT ATGCTTCCAA CATCCCTGTG 
TACCGCTTCG TGCAGCGCCC CGGAGACCTC GTGTGGATTA ATGCGGGCAC CGTGCACTGG 
GTGCAGGCCA CTGGCTGGTG CAACAACATC GCCTGGAACG TGGGGCCCCT CACCGCCTAT 
CAGTACCAGC TGGCCCTGGA ACGATACGAG TGGAACGAGG TGAAGAACGT CAAATCCATC 
GTGCCCATGA TTCACGTATC CTGGAATGTG GCTCGTACGG TCAAAATCAG CGACCCGGAC 
TTGTTCAGGA TGATCAAGTT CTGCCTCCTG CAGTCCATGA AGCACTGCCA GGTGCAACGC 
GAGAGCCTGG TGCGGGCCGG GAAGAAGATC GCCTACCAGG GCCGGGTCAA GGACGAGCCC 
GCCTACTACT GCAACGAGTG CGACGTGGAG GTGTTCAACA TCCTGTTTGT GACGAGCGAG 
AACGGCAGCC GCAACACGTA CCTCGTGCAC TGCGAGGCCT GTGCGCGGCG CCGCAGTGCG 
GGCCTACAGG GCGTGGTGGT GCTGGAGCAG TACCGCACCG AGGAGCTGGC GCAGGCCTAC 
GACGCCTTCA CGCTGGCCCC CGCCAGCACG TCGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969020.1
CDS	520..5478
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_014113545.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris lysine demethylase 6B (KDM6B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113545.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGTGCACC GGACCCGAAG AACCATCACA TCATCAAGTT TGGCACCAAC 
ATCGACCTAT CCGATGCCAA GAGGTGGAAG CCCCAGCTGC AGGAGCTGCT CAAGCTGCCC 
GCTTTCATGC GGGTCACATC CACTGGCAAC ATGCTGAGCC ACGTGGGCCA CACCATCCTG 
GGCATGAACA CCGTGCAGCT GTACATGAAG GTCCCTGGCA GTCGAACTCC AGGCCACCAG 
GAGAACAACA ACTTCTGCTC CGTCAACATC AACATCGGGC CAGGCGACTG CGAGTGGTTC 
GCGGTGCACG AACACTACTG GGAGACCATC AGCGCCTTCT GCGACCGGCA CGGCGTGGAC 
TACCTGACCG GTTCCTGGTG GCCAATCCTG GATGACCTCT ATGCTTCCAA CATCCCTGTG 
TACCGCTTCG TGCAGCGCCC CGGAGACCTC GTGTGGATTA ATGCGGGCAC CGTGCACTGG 
GTGCAGGCCA CTGGCTGGTG CAACAACATC GCCTGGAACG TGGGGCCCCT CACCGCCTAT 
CAGTACCAGC TGGCCCTGGA ACGATACGAG TGGAACGAGG TGAAGAACGT CAAATCCATC 
GTGCCCATGA TTCACGTATC CTGGAATGTG GCTCGTACGG TCAAAATCAG CGACCCGGAC 
TTGTTCAGGA TGATCAAGTT CTGCCTCCTG CAGTCCATGA AGCACTGCCA GGTGCAACGC 
GAGAGCCTGG TGCGGGCCGG GAAGAAGATC GCCTACCAGG GCCGGGTCAA GGACGAGCCC 
GCCTACTACT GCAACGAGTG CGACGTGGAG GTGTTCAACA TCCTGTTTGT GACGAGCGAG 
AACGGCAGCC GCAACACGTA CCTCGTGCAC TGCGAGGCCT GTGCGCGGCG CCGCAGTGCG 
GGCCTACAGG GCGTGGTGGT GCTGGAGCAG TACCGCACCG AGGAGCTGGC GCAGGCCTAC 
GACGCCTTCA CGCTGGCCCC CGCCAGCACG TCGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_014113545.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013969020.1 Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X11, mRNA.

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CloneID	OCe36421
Clone ID Related Accession (Same CDS sequence)	XM_014113545.2 , XM_014113545.1
Accession Version	XM_014113545.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysine-specific demethylase 6B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGTGCACC GGACCCGAAG AACCATCACA TCATCAAGTT TGGCACCAAC 
ATCGACCTAT CCGATGCCAA GAGGTGGAAG CCCCAGCTGC AGGAGCTGCT CAAGCTGCCC 
GCTTTCATGC GGGTCACATC CACTGGCAAC ATGCTGAGCC ACGTGGGCCA CACCATCCTG 
GGCATGAACA CCGTGCAGCT GTACATGAAG GTCCCTGGCA GTCGAACTCC AGGCCACCAG 
GAGAACAACA ACTTCTGCTC CGTCAACATC AACATCGGGC CAGGCGACTG CGAGTGGTTC 
GCGGTGCACG AACACTACTG GGAGACCATC AGCGCCTTCT GCGACCGGCA CGGCGTGGAC 
TACCTGACCG GTTCCTGGTG GCCAATCCTG GATGACCTCT ATGCTTCCAA CATCCCTGTG 
TACCGCTTCG TGCAGCGCCC CGGAGACCTC GTGTGGATTA ATGCGGGCAC CGTGCACTGG 
GTGCAGGCCA CTGGCTGGTG CAACAACATC GCCTGGAACG TGGGGCCCCT CACCGCCTAT 
CAGTACCAGC TGGCCCTGGA ACGATACGAG TGGAACGAGG TGAAGAACGT CAAATCCATC 
GTGCCCATGA TTCACGTATC CTGGAATGTG GCTCGTACGG TCAAAATCAG CGACCCGGAC 
TTGTTCAGGA TGATCAAGTT CTGCCTCCTG CAGTCCATGA AGCACTGCCA GGTGCAACGC 
GAGAGCCTGG TGCGGGCCGG GAAGAAGATC GCCTACCAGG GCCGGGTCAA GGACGAGCCC 
GCCTACTACT GCAACGAGTG CGACGTGGAG GTGTTCAACA TCCTGTTTGT GACGAGCGAG 
AACGGCAGCC GCAACACGTA CCTCGTGCAC TGCGAGGCCT GTGCGCGGCG CCGCAGTGCG 
GGCCTACAGG GCGTGGTGGT GCTGGAGCAG TACCGCACCG AGGAGCTGGC GCAGGCCTAC 
GACGCCTTCA CGCTGGCCCC CGCCAGCACG TCGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013969020.1
CDS	526..5484
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASIGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRIGRLQQAQFWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRSYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGDEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDVWGPDRKGSVPPERQEQRHPLPHPYPYPVPAYTTHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTTSSSSSSSSSSSSSSNTGLRGAEPNPGIPGADHYQTPALDISSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPPSPPPPPCPRLLRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLLHREGFLGPPAPRFSVGTQDSHTPPTPPATSSSSSNHGSHSSSPTGPVSFPPPPYLARSLDPLPRPPSPTLSPQDPPLAPLTLALPPAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKAPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGVGPPPPLKEPFPSLQPSFPSDTAPATTAAPAATATTTTATQEEEKKPPPVLPPPPPLAKFPPPQPQPPPPPPLPPAASPASLLKSLASVLEGQKYCYRGTGAAVATRPGPLPTTQYSPGPPSGATAPPPASAAPSARGSPQPSASSSSQFSTSGGPWARERRAGEEPARGPTAPAPPPPPPPPLPLPPARSESEVLEEISRACETLVERVGRSAADLAGPVDTAGPVDSGTERLLPPAQAREEGGGAVAAAGPGSGKRRQKEHQKEHQKEHRRHRRACKDSTGRRPREGRVKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPAAPAPPPAPAPAAQPTPPSAPTPGKKAREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFRMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_014113545.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris lysine (K)-specific demethylase 6B (KDM6B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113545.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGTAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC ATCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ATGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACC 
CCCACCCCGA GACCCCTCCA TGGGAAGCTG GAATCTCTGC ATGGCTGCGT GCAGGCATTG 
CTCCGAGAGC CAGCTCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCCGAG 
CACGATAGTG AGGAGGCCAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GGCCCCGCAT CGGGCGACTA CAGCAGGCCC AGTTCTGGAA CTTTCATGCT 
GGCTCCTGCC AGCACCGAGC CAAGGTCCTG CCCCCACTGG AGCAAGTGTG GAACTTGCTG 
CACCTCGAGC ACAAGCGGAG CTATGGGGCC AAGCGGGGAG GCCCCCCAGT GAAGCGAGCT 
GCTGAACCCC CTGTGGTACA GCCTGTGCCT CCCGCAGCGC TCTCAGGCCC CTCGGGGGAC 
GAGGGCCTCA GTCCTGGAGG CAAGCGCAGG AGAGGCTGCA GCTCAGAGCA GACTGGCCTT 
CCCCCAGGGC TGCCGTTGCC TCCACCACCA CTGCCACCGC CGCCACCACC GCCACCGCCC 
CCCCCACCCC TGCCTGGCCT AGCCACTAGC CCTCCATTTC AGCTGACCAA GCCAGGGCTG 
TGGAGTACCC TGCATGGAGA CGTCTGGGGC CCTGACCGCA AGGGTTCGGT GCCCCCAGAA 
CGCCAGGAGC AGCGGCACCC GCTGCCCCAC CCGTATCCAT ACCCGGTTCC GGCCTATACC 
ACGCACCCCC CTGGCCACCG GCTGGTCCCG GCCGCACCCC CAGGCCCCGG CCCCCGCCCC 
CCAGGAGCAG AGAGCCATGG CTGCCCGCCT GCCACCCGTC CCCCCGGAAG TGACCTTAGA 
GAGAGCAGAG TTCAGAGGTC GCGGATGGAC TCCAGCGTTT CACCAGCAGC AACCACCGCC 
TGCGTGCCTT ACGCCCCTTC CCGGCCCCCT GGCCTCCCCG GCACCACCAC CAGCAGCAGC 
AGTAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAACACCG GTCTCCGGGG CGCGGAGCCG 
AACCCAGGCA TCCCCGGCGC TGACCATTAC CAAACTCCCG CGCTGGACAT CTCCTCCCAC 
CAAGGCCGCC TGGGGCCCTC GGCGCACAGC AGTCGGAAAC CGTTCCTGGC GGCTCCCGCT 
GCCACTCCTC ACCTGTCCCT GCCACCTGGA CCCCCGTCAC CTCCTCCACC ACCCTGTCCC 
CGCCTCCTAC GCCCCCCACC ACCTCCTGCC TGGCTGAAGG GCCCGGCTTG CCGGGCAGCC 
CGCGAGGATG GGGAGATCTT AGAGGAGCTC TTCTTTGGGG CTGAGGGACG CCCCCGCCCT 
CCCCCACCAC CCCTCCTTCA CCGCGAGGGC TTCTTGGGGC CTCCGGCTCC CCGCTTTTCT 
GTGGGCACTC AGGATTCTCA CACCCCTCCC ACCCCCCCAG CCACCAGCAG CAGCAGCAGC 
AACCATGGCA GCCACAGCAG CAGCCCTACG GGGCCTGTGT CCTTCCCTCC GCCTCCCTAT 
CTGGCCAGAA GTTTGGACCC CCTTCCGCGG CCCCCCAGCC CCACACTGAG CCCCCAGGAC 
CCACCTCTTG CACCCTTGAC TCTTGCCCTG CCTCCAGCTC CTCCCTCCTC CTGCCACCAA 
AATACCTCAG GAAGCTTCAG GCGCCCGGAG AGCCCTCGGC CCAGGGTCTC CTTCCCAAAG 
ACCCCCGAGG TGGGGCCGGG GCCATCCCCA GGCCCACTGA ATAAAGCCCC CCAGCCTGTG 
CCGCCCAGGG TTGGGGAGCT GCCTGCCCGA GGCCCACGGC TCTTCGATTT TCCCCCTACC 
CCACTGGAGG ACCAGTTTGA GGAGCCGGCT GAATTCAAGA TCCTCCCTGA CGGGCTGGCC 
AACATCATGA AGATGCTGGA CGAATCCATT CGCAAGGAGG AGGAGCAGCA GCAACAGGAG 
GCAGGCGTGG GCCCCCCGCC CCCGCTGAAG GAGCCCTTTC CATCTCTGCA GCCTTCATTC 
CCCAGTGACA CAGCCCCAGC CACCACGGCA GCCCCGGCAG CCACCGCTAC CACCACCACT 
GCCACCCAGG AAGAAGAGAA GAAGCCACCA CCAGTCCTGC CACCGCCGCC GCCTCTTGCG 
AAGTTCCCAC CGCCCCAGCC CCAGCCCCCA CCGCCGCCCC CGCTACCCCC AGCAGCCAGC 
CCAGCCAGCC TGCTCAAATC CTTGGCCTCC GTGCTGGAGG GACAGAAGTA CTGTTACCGG 
GGGACCGGAG CAGCTGTTGC CACCCGGCCC GGGCCCTTGC CCACCACGCA GTATTCCCCC 
GGCCCCCCGT CAGGTGCTAC CGCCCCGCCG CCTGCCTCGG CGGCCCCCAG CGCCCGGGGC 
TCCCCGCAGC CCTCTGCTTC CTCGTCATCT CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC 
CGGGAGCGCA GGGCGGGCGA AGAGCCAGCC CGAGGCCCCA CAGCCCCTGC CCCACCGCCG 
CCACCGCCCC CACCCCTGCC TCTGCCTCCT GCTCGATCTG AGTCTGAGGT GCTAGAAGAG 
ATCAGTCGGG CTTGTGAGAC CCTCGTGGAA CGGGTGGGCC GGAGCGCCGC AGACCTGGCC 
GGCCCGGTGG ACACGGCAGG CCCGGTGGAC AGTGGGACAG AGCGGCTGTT GCCTCCTGCC 
CAGGCCCGGG AGGAGGGTGG CGGGGCAGTG GCAGCAGCAG GACCTGGCAG TGGCAAGCGG 
CGGCAGAAGG AGCATCAGAA GGAGCACCAG AAGGAGCATC GGCGGCACCG GCGAGCTTGT 
AAGGACAGCA CGGGTCGGCG GCCCCGAGAG GGCAGAGTGA AGACCAAGGC CAAGGCCCCC 
AAAGAAAAGA GCCGCCGGGT GCTGGGCAAC CTGGACCTGC AGAGTGAGGA GATCCAGGGG 
CGTGAGAAGG CTCGGCCCGA TCTCGGCGGG GCCTCCAAGG CCAAGCCACC CGCAGCCCCG 
GCCCCTCCAC CAGCTCCTGC ACCTGCTGCC CAGCCCACAC CGCCCTCCGC TCCCACACCA 
GGGAAGAAGG CCCGGGAGGA AGCGCCGGGG CCCCCTGGGG TCAGCCGGGC TGACATGCTG 
AAGCTGCGCT CGCTTAGTGA GGGGCCCCCC AAGGAGCTGA AGATCCGCCT CATCAAGGTG 
GAGAGCGGTG ACAAGGAGAC CTTTATTGCC TCTGAGGTGG AAGAGCGGAG GCTGCGCATG 
GCGGACCTTA CCATCAGCCA CTGCGCTGCC GACGTCGTGC GGGCCAGCAA GAACGCCAAG 
GTGAAAGGGA AGTTTCGAGA GTCCTACCTT TCCCCCGCCC AGTCTGTGAA ACCCAAGATC 
AACACTGAGG AGAAGCTACC CCGGGAAAAA CTCAACCCAC CCACCCCCAG CATCTATCTG 
GAGAGCAAAC GGGACGCCTT CTCACCCGTG CTACTGCAGT TCTGTACGGA CCCTCGAAAT 
CCCATCACCG TGATCCGGGG CCTGGCGGGC TCCCTGCGGC TCAACTTGGG CCTCTTCTCC 
ACCAAGACGC TGGTGGAGGC AAGCGGCGAG CACACGGTGG AGGTGCGCAC GCAGGTGCAG 
CAGCCCTCCG ATGAGAACTG GGATCTGACG GGCACGCGGC AGATCTGGCC CTGCGAGAGC 
TCCCGTTCCC ATACCACCAT TGCCAAGTAT GCGCAGTACC AGGCCTCATC CTTTCAGGAG 
TCCCTGCAGG AGGAGAAGGA GAGTGAGGAT GAGGAATCTG AGGAGCCCGA CAGCACCACA 
GGAACCCCTC CCAGTGCACC GGACCCGAAG AACCATCACA TCATCAAGTT TGGCACCAAC 
ATCGACCTAT CCGATGCCAA GAGGTGGAAG CCCCAGCTGC AGGAGCTGCT CAAGCTGCCC 
GCTTTCATGC GGGTCACATC CACTGGCAAC ATGCTGAGCC ACGTGGGCCA CACCATCCTG 
GGCATGAACA CCGTGCAGCT GTACATGAAG GTCCCTGGCA GTCGAACTCC AGGCCACCAG 
GAGAACAACA ACTTCTGCTC CGTCAACATC AACATCGGGC CAGGCGACTG CGAGTGGTTC 
GCGGTGCACG AACACTACTG GGAGACCATC AGCGCCTTCT GCGACCGGCA CGGCGTGGAC 
TACCTGACCG GTTCCTGGTG GCCAATCCTG GATGACCTCT ATGCTTCCAA CATCCCTGTG 
TACCGCTTCG TGCAGCGCCC CGGAGACCTC GTGTGGATTA ATGCGGGCAC CGTGCACTGG 
GTGCAGGCCA CTGGCTGGTG CAACAACATC GCCTGGAACG TGGGGCCCCT CACCGCCTAT 
CAGTACCAGC TGGCCCTGGA ACGATACGAG TGGAACGAGG TGAAGAACGT CAAATCCATC 
GTGCCCATGA TTCACGTATC CTGGAATGTG GCTCGTACGG TCAAAATCAG CGACCCGGAC 
TTGTTCAGGA TGATCAAGTT CTGCCTCCTG CAGTCCATGA AGCACTGCCA GGTGCAACGC 
GAGAGCCTGG TGCGGGCCGG GAAGAAGATC GCCTACCAGG GCCGGGTCAA GGACGAGCCC 
GCCTACTACT GCAACGAGTG CGACGTGGAG GTGTTCAACA TCCTGTTTGT GACGAGCGAG 
AACGGCAGCC GCAACACGTA CCTCGTGCAC TGCGAGGCCT GTGCGCGGCG CCGCAGTGCG 
GGCCTACAGG GCGTGGTGGT GCTGGAGCAG TACCGCACCG AGGAGCTGGC GCAGGCCTAC 
GACGCCTTCA CGCTGGCCCC CGCCAGCACG TCGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KDM6B
Entrez Gene ID	489481
Full Name	lysine demethylase 6B
Synonyms	JMJD3
General protein information	Preferred Names lysine demethylase 6B Names lysine-specific demethylase 6B jumonji domain containing 3
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Mus musculus (house mouse)	Kdm6b	NP_001017426.1
Pan troglodytes (chimpanzee)	KDM6B	XP_003315411.1
Rattus norvegicus (Norway rat)	Kdm6b	NP_001102299.1
Canis lupus familiaris (dog)	KDM6B	XP_005620054.1
Bos taurus (cattle)	KDM6B	XP_003587460.2
Homo sapiens (human)	KDM6B	NP_001073893.1
	KDM6B	XP_001110616.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KDM6B cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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