KDM6B gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015458745.1	XP_015314231.1	lysine-specific demethylase 6B isoform X1
XM_015458746.1	XP_015314232.1	lysine-specific demethylase 6B isoform X1
XM_015458747.1	XP_015314233.1	lysine-specific demethylase 6B isoform X1
XM_015468043.1	XP_015323529.1	lysine-specific demethylase 6B isoform X2
XM_015468042.1	XP_015323528.1	lysine-specific demethylase 6B isoform X1
XM_010816060.3	XP_010814362.2	lysine-specific demethylase 6B isoform X1
XM_010816062.3	XP_010814364.2	lysine-specific demethylase 6B isoform X1
XM_010816063.3	XP_010814365.2	lysine-specific demethylase 6B isoform X1
XM_024980742.1	XP_024836510.1	lysine-specific demethylase 6B isoform X1
XM_010816061.2	XP_010814363.1	lysine-specific demethylase 6B isoform X1
XM_010816062.2	XP_010814364.1	lysine-specific demethylase 6B isoform X1
XM_010816063.2	XP_010814365.1	lysine-specific demethylase 6B isoform X1
XM_015458748.1	XP_015314234.1	lysine-specific demethylase 6B isoform X2
XM_015458745.2	XP_015314231.2	lysine-specific demethylase 6B isoform X1
XM_024980743.1	XP_024836511.1	lysine-specific demethylase 6B isoform X2
XM_010816060.2	XP_010814362.1	lysine-specific demethylase 6B isoform X1
XM_015468034.1	XP_015323520.1	lysine-specific demethylase 6B isoform X1
XM_015468037.1	XP_015323523.1	lysine-specific demethylase 6B isoform X1
XM_015468039.1	XP_015323525.1	lysine-specific demethylase 6B isoform X1
XM_015468036.1	XP_015323522.1	lysine-specific demethylase 6B isoform X1
XM_015468038.1	XP_015323524.1	lysine-specific demethylase 6B isoform X1
XM_010816059.2	XP_010814361.2	lysine-specific demethylase 6B isoform X1
XM_010816061.3	XP_010814363.2	lysine-specific demethylase 6B isoform X1
XM_024980741.1	XP_024836509.1	lysine-specific demethylase 6B isoform X1
XM_015458746.2	XP_015314232.2	lysine-specific demethylase 6B isoform X1
XM_015468035.1	XP_015323521.1	lysine-specific demethylase 6B isoform X1
XM_010816059.1	XP_010814361.1	lysine-specific demethylase 6B isoform X1
XM_003587412.5	XP_003587460.3	lysine-specific demethylase 6B isoform X1
XM_003587412.4	XP_003587460.2	lysine-specific demethylase 6B isoform X1
XM_015468040.1	XP_015323526.1	lysine-specific demethylase 6B isoform X1
XM_015468041.1	XP_015323527.1	lysine-specific demethylase 6B isoform X1
XM_024980740.1	XP_024836508.1	lysine-specific demethylase 6B isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226773	XM_010816061.3 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226773	XM_024980740.1 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226773	XM_010816059.2 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226773	XM_010816062.3 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226773	XM_015458746.2 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226773	XM_003587412.5 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226773	XM_024980742.1 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226773	XM_010816060.3 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226773	XM_024980741.1 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226773	XM_010816063.3 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226773	XM_015458745.2 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa226774	XM_024980743.1 Latest version!	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa29804	XM_015458745.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015458746.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_010816059.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015468041.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015458747.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015468042.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015468036.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_010816063.2 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015468038.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015468035.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015468034.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_010816061.2 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015468039.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_010816062.2 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_010816060.2 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_003587412.4 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015468040.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa29804	XM_015468037.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa93888	XM_015458748.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OBa93888	XM_015468043.1 Latest version!	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

KDM6B ( XM_010816060.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814362.2 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_010816060.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816060.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814362.2
CDS	792..5726
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_010816060.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816060.3

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_010816062.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814364.2 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X4, mRNA.

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CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_010816062.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816062.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814364.2
CDS	539..5473
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_010816062.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816062.3

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_010816063.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814365.2 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_010816063.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816063.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814365.2
CDS	421..5355
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_010816063.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816063.3

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_024980742.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836510.1 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_024980742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836510.1
CDS	443..5377
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_024980742.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980742.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015458745.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314231.2 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X8, mRNA.

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CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_015458745.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314231.2
CDS	424..5358
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015458745.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458745.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_010816059.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814361.2 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X2, mRNA.

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CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_010816059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814361.2
CDS	468..5402
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_010816059.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816059.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_010816061.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814363.2 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X5, mRNA.

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CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_010816061.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816061.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814363.2
CDS	412..5346
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_010816061.3
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816061.3

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_024980741.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836509.1 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X7, mRNA.

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CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_024980741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836509.1
CDS	788..5722
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_024980741.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980741.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015458746.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314232.2 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_015458746.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314232.2
CDS	1351..6285
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015458746.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458746.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_003587412.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587460.3 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_003587412.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587412.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587460.3
CDS	543..5477
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_003587412.5
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587412.5

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_024980740.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836508.1 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X6, mRNA.

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CloneID	OBa226773
Clone ID Related Accession (Same CDS sequence)	XM_010816060.3 , XM_010816062.3 , XM_010816063.3 , XM_024980742.1 , XM_015458745.2 , XM_010816059.2 , XM_010816061.3 , XM_024980741.1 , XM_015458746.2 , XM_003587412.5 , XM_024980740.1
Accession Version	XM_024980740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836508.1
CDS	464..5398
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_024980740.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980740.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCTCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACAGCC 
ACCCAGGAAG AGGAGAAGAA GCCACCAGCC CTGCCACCAC CGCCGCCTCT AGCCAAGTTC 
CCCCCACCAC CCCAGCCACA GCCACCGCCA CCCCCGCTCC CCCCACCAGC CAGCCCGGCC 
AGCCTGCTCA AGTCCTTGGC CTCCGTGCTG GAGGGACAAA AGTACTGTTA CCGAGGGACT 
GGAGCTGCTG TTCCCACCCG GCCTGGGTCC TTGCCCACCA CTCAGTATTC CCCTGGTCCC 
TCATCAGGTG CTACCGCCCC GCCACCCACC TCAGTGGCCC CGAGCGCCCA GGGCTCCCCA 
CAGCCCTCCG CTTCCTCGTC ATCTCAGTTC TCTACCTCAG GCGGGCCCTG GGCCCGGGAG 
CGCAGGGCCA GCGAAGAGCC AGCCCCAGGC CCCATGGCCC CCGCCCCGCC GCCCCCACCC 
CTGCCTCTGC CCCCTGCTCG CTCTGAGTCT GAGGTGCTAG AAGAGATCAG TCGGGCTTGT 
GAGACCCTTG TGGAGCGGGT AGGCCGGAGT GCCACGGACC CGGCAGACCT GGTGGACACA 
GCAGATGCAG CGGACAGTGG AGCTGAGCGA TTGCTGCCTC CAGCTCCGGC CAAGGAGGAG 
AGCGTTGGGG TGGCAGCTGC GGCAGGACCC GGGGGCAGCA AGCGGCGGCA GAAGGAGCAC 
CAGAAAGAGC ACCGGCGGCA CAGGCGGGCC TGCAAGGACA GTGTGGGGCG GCGGCCCCGT 
GAGGGCAGGG CCAAGACCAA GGCCAAGGCC CCCAAAGAAA AGAGCCGCAG GGTGCTGGGG 
AACCTGGACT TGCAGAGCGA GGAGATCCAG GGTCGTGAGA AAGCCCGGCC AGATCTTGGT 
GGGGCCTCCA AGGCCAAGCC ACCCACAGCT CCAGGCCCTC CACCTGCTCC TGCCCCCTCT 
GCCCAGCCCA CACCCCCGGC AGCCCCTGCC CCTGGGAAGA AAGCTCGAGA GGAAGCTTCA 
GGGCCACCAG GGGTCAGCCG GGCTGACATG CTGAAGCTGC GCTCACTCAG CGAAGGGCCC 
CCCAAGGAGC TGAAGATCCG GCTCATCAAG GTAGAGAGCG GTGACAAGGA GACCTTCATC 
GCCTCCGAGG TGGAAGAGCG GCGGCTGCGG ATGGCAGACC TTACCATCAG CCACTGTGCT 
GCTGACGTTG TGCGTGCCAG CAAGAACGCC AAGGTGAAAG GGAAGTTTCG AGAGTCCTAC 
CTGTCCCCTG CCCAGTCTGT GAAACCGAAG ATCAACACTG AGGAAAAGCT GCCCCGGGAA 
AAACTCAACC CACCCACACC TAGCATCTAT CTGGAAAGCA AACGGGATGC CTTCTCGCCG 
GTGCTGTTAC AGTTCTGTAC AGACCCTCGA AATCCCATCA CCGTGATCCG GGGCCTGGCA 
GGCTCCCTGC GGCTCAACTT GGGCCTCTTC TCCACCAAGA CTCTGGTGGA GGCGAGTGGG 
GAGCACACAG TGGAGGTGCG CACCCAGGTG CAGCAGCCCT CGGATGAGAA CTGGGATCTG 
ACGGGCACAC GACAGATCTG GCCCTGCGAG AGCTCCCGTT CCCATACCAC CATTGCCAAG 
TACGCGCAGT ACCAGGCCTC ATCCTTCCAG GAATCCCTGC AGGAGGAGAA GGAGAGTGAG 
GACGAGGAGT CAGAGGAGCC GGACAGCACC ACAGGAACCC CTCCTAGCAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_024980743.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836511.1 Bos taurus lysine demethylase 6B (KDM6B), transcript variant X12, mRNA.

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CloneID	OBa226774
Clone ID Related Accession (Same CDS sequence)	XM_024980743.1
Accession Version	XM_024980743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GGAGCAGCGG 
CACTCGCTGC CTCACTCATA TCCATACCCG GCTCCTGCTT ACCCTGCTCA CCCCCCTGGC 
CACCGGCTGG TCCCGGCTGC TCCCCCAGGC CCAGGCCCCC GCCCCCCAGG AGCAGAGAGC 
CATGGCTGCC CGCCTGCCAC CCGTCCCCCC GGAAGTGACC TTAGAGAGAG CAGAGTTCAG 
AGGTCGCGGA TGGACTCCAG CGTTTCACCA GCAGCAACCA CCGCCTGCGT GCCTTACGCC 
CCTTCCCGGC CCCCCGGCCT CCCCGGCACC ACCAGCAGCA GCAGCAGCAA CACTGGTCCC 
CGTGGCGTGG AGCCGAGCCC AGGCATTCCC GGCGCTGACC ATTACCAGAC ACCCGCGCTG 
GAGGTCTCCT CTCACCAAGG CCGCCTGGGG CCCTCGGCAC ACAGTAGTCG GAAACCCTTC 
CTGGCGGCTC CTGCTGCCAC TCCCCACCTG TCTCTGCCAC CTGGCCCCAC CTCACCTCCT 
CCACCCTCTT GTCCCCGCCT CATACGCCCC CCACCCCCTC CTGCCTGGCT GAAGGGCCCA 
GCCTGCCGGG CAGCTCGTGA AGATGGAGAG ATCTTAGAGG AGCTCTTCTT CGGGGCTGAG 
GGACGCCCCC GCCCTCCCCC CCCTCCACTT CCCCACCGCG AGGGCTTCTT GGGGCCTCCG 
GCCCCCCGCT TTTCTGTGGG CACTCAGGAT TCGCACACCC CTCCCACTCC CCCAACCACC 
AGCAGCAGCA GCAGCAACAA TGGCAGCCAC AGCAGCAGCC CTACTGGGCC TGTGTCCTTC 
CCCCCACCTC CTTATCTGGC CAGAAGTATG GACTCCCTTC CCCGGCCCCC CAGCCCCACA 
CTGAGCCCCC AGGACCCACC CCTTGCACCC CTGACTCTTG CCCTGCCTTC AGCCCCTCCC 
TCCTCTTGCC ACCAAAATAC CTCAGGAAGC TTCAGGCGCC CGGAGAGCCC TCGGCCCAGG 
GTCTCCTTCC CAAAGACCCC CGAGGTGGGG CCGGGGCCAT CCCCAGGCCC CCTGAATAAA 
ACCCCCCAGC CTGTGCCGCC CAGGGTCGGG GAGCTGCCTG CCCGAGGCCC TCGACTTTTT 
GATTTCCCTC CTACCCCTCT GGAGGATCAG TTTGAGGAGC CAGCTGAATT CAAGATCCTA 
CCTGATGGGC TGGCTAATAT CATGAAGATG CTGGATGAAT CCATTCGCAA GGAGGAGGAG 
CAGCAACAAC AGGAGGCAGG CGCGGTCCCC GTCCCCCCGC CTCCTCTGAA GGAGCCCTTT 
GCATCTCTGC AGCCTCCGTT CCCCACAGAC ACAGCCCCAG CCACCACTGC TGCCGCCGCC 
GCCACCGCCA CCACCACCAC CACCACCACC ACAGCCACCC AGGAAGAGGA GAAGAAGCCA 
CCAGCCCTGC CACCACCGCC GCCTCTAGCC AAGTTCCCCC CACCACCCCA GCCACAGCCA 
CCGCCACCCC CGCTCCCCCC ACCAGCCAGC CCGGCCAGCC TGCTCAAGTC CTTGGCCTCC 
GTGCTGGAGG GACAAAAGTA CTGTTACCGA GGGACTGGAG CTGCTGTTCC CACCCGGCCT 
GGGTCCTTGC CCACCACTCA GTATTCCCCT GGTCCCTCAT CAGGTGCTAC CGCCCCGCCA 
CCCACCTCAG TGGCCCCGAG CGCCCAGGGC TCCCCACAGC CCTCCGCTTC CTCGTCATCT 
CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC CGGGAGCGCA GGGCCAGCGA AGAGCCAGCC 
CCAGGCCCCA TGGCCCCCGC CCCGCCGCCC CCACCCCTGC CTCTGCCCCC TGCTCGCTCT 
GAGTCTGAGG TGCTAGAAGA GATCAGTCGG GCTTGTGAGA CCCTTGTGGA GCGGGTAGGC 
CGGAGTGCCA CGGACCCGGC AGACCTGGTG GACACAGCAG ATGCAGCGGA CAGTGGAGCT 
GAGCGATTGC TGCCTCCAGC TCCGGCCAAG GAGGAGAGCG TTGGGGTGGC AGCTGCGGCA 
GGACCCGGGG GCAGCAAGCG GCGGCAGAAG GAGCACCAGA AAGAGCACCG GCGGCACAGG 
CGGGCCTGCA AGGACAGTGT GGGGCGGCGG CCCCGTGAGG GCAGGGCCAA GACCAAGGCC 
AAGGCCCCCA AAGAAAAGAG CCGCAGGGTG CTGGGGAACC TGGACTTGCA GAGCGAGGAG 
ATCCAGGGTC GTGAGAAAGC CCGGCCAGAT CTTGGTGGGG CCTCCAAGGC CAAGCCACCC 
ACAGCTCCAG GCCCTCCACC TGCTCCTGCC CCCTCTGCCC AGCCCACACC CCCGGCAGCC 
CCTGCCCCTG GGAAGAAAGC TCGAGAGGAA GCTTCAGGGC CACCAGGGGT CAGCCGGGCT 
GACATGCTGA AGCTGCGCTC ACTCAGCGAA GGGCCCCCCA AGGAGCTGAA GATCCGGCTC 
ATCAAGGTAG AGAGCGGTGA CAAGGAGACC TTCATCGCCT CCGAGGTGGA AGAGCGGCGG 
CTGCGGATGG CAGACCTTAC CATCAGCCAC TGTGCTGCTG ACGTTGTGCG TGCCAGCAAG 
AACGCCAAGG TGAAAGGGAA GTTTCGAGAG TCCTACCTGT CCCCTGCCCA GTCTGTGAAA 
CCGAAGATCA ACACTGAGGA AAAGCTGCCC CGGGAAAAAC TCAACCCACC CACACCTAGC 
ATCTATCTGG AAAGCAAACG GGATGCCTTC TCGCCGGTGC TGTTACAGTT CTGTACAGAC 
CCTCGAAATC CCATCACCGT GATCCGGGGC CTGGCAGGCT CCCTGCGGCT CAACTTGGGC 
CTCTTCTCCA CCAAGACTCT GGTGGAGGCG AGTGGGGAGC ACACAGTGGA GGTGCGCACC 
CAGGTGCAGC AGCCCTCGGA TGAGAACTGG GATCTGACGG GCACACGACA GATCTGGCCC 
TGCGAGAGCT CCCGTTCCCA TACCACCATT GCCAAGTACG CGCAGTACCA GGCCTCATCC 
TTCCAGGAAT CCCTGCAGGA GGAGAAGGAG AGTGAGGACG AGGAGTCAGA GGAGCCGGAC 
AGCACCACAG GAACCCCTCC TAGCAGTGCA CCAGACCCGA AGAACCATCA CATCATCAAG 
TTTGGCACCA ACATCGACCT GTCTGATGCT AAGCGGTGGA AGCCCCAGCT GCAGGAGCTA 
CTGAAGCTGC CCGCCTTCAT GCGTGTCACC TCCACGGGGA ACATGCTGAG CCACGTGGGC 
CACACCATCC TGGGCATGAA CACGGTGCAG CTGTACATGA AGGTGCCCGG CAGCCGAACG 
CCAGGCCATC AAGAGAACAA CAACTTCTGC TCGGTCAACA TCAACATTGG CCCAGGAGAC 
TGCGAGTGGT TCGCGGTGCA CGAGCACTAC TGGGAGACCA TCAGCGCCTT CTGCGACCGG 
CACGGCGTGG ACTACCTGAC GGGTTCCTGG TGGCCAATCC TGGATGACCT CTATGCTTCC 
AACATCCCTG TGTACCGCTT CGTGCAGCGC CCCGGAGACC TGGTGTGGAT TAATGCGGGC 
ACCGTGCACT GGGTGCAGGC CACCGGCTGG TGTAACAACA TCGCCTGGAA CGTGGGGCCC 
CTCACCGCCT ATCAGTACCA GCTGGCCCTG GAACGATATG AGTGGAATGA GGTGAAGAAT 
GTCAAATCCA TTGTGCCCAT GATTCACGTG TCCTGGAACG TGGCTCGCAC GGTCAAAATC 
AGCGACCCCG ACTTGTTCAA GATGATCAAG TTCTGCCTCC TGCAGTCCAT GAAGCACTGC 
CAGGTGCAGC GGGAGAGCCT GGTGCGGGCC GGGAAGAAAA TTGCCTACCA GGGCCGGGTC 
AAGGATGAGC CCGCCTACTA CTGCAACGAG TGCGACGTGG AGGTGTTCAA CATCCTGTTC 
GTGACGAGCG AGAACGGCAG CCGCAACACG TACCTGGTGC ACTGCGAGGC GTGCGCGCGG 
CGCCGCAGCG CGGGCCTGCA GGGCGTGGTG GTGTTGGAGC AATACCGCAC CGAAGAGCTG 
GCGCAGGCCT ATGACGCCTT CACGCTGGCC CCCGCCAGCA CGTCGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836511.1
CDS	543..5273
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_024980743.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine demethylase 6B (KDM6B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980743.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GGAGCAGCGG 
CACTCGCTGC CTCACTCATA TCCATACCCG GCTCCTGCTT ACCCTGCTCA CCCCCCTGGC 
CACCGGCTGG TCCCGGCTGC TCCCCCAGGC CCAGGCCCCC GCCCCCCAGG AGCAGAGAGC 
CATGGCTGCC CGCCTGCCAC CCGTCCCCCC GGAAGTGACC TTAGAGAGAG CAGAGTTCAG 
AGGTCGCGGA TGGACTCCAG CGTTTCACCA GCAGCAACCA CCGCCTGCGT GCCTTACGCC 
CCTTCCCGGC CCCCCGGCCT CCCCGGCACC ACCAGCAGCA GCAGCAGCAA CACTGGTCCC 
CGTGGCGTGG AGCCGAGCCC AGGCATTCCC GGCGCTGACC ATTACCAGAC ACCCGCGCTG 
GAGGTCTCCT CTCACCAAGG CCGCCTGGGG CCCTCGGCAC ACAGTAGTCG GAAACCCTTC 
CTGGCGGCTC CTGCTGCCAC TCCCCACCTG TCTCTGCCAC CTGGCCCCAC CTCACCTCCT 
CCACCCTCTT GTCCCCGCCT CATACGCCCC CCACCCCCTC CTGCCTGGCT GAAGGGCCCA 
GCCTGCCGGG CAGCTCGTGA AGATGGAGAG ATCTTAGAGG AGCTCTTCTT CGGGGCTGAG 
GGACGCCCCC GCCCTCCCCC CCCTCCACTT CCCCACCGCG AGGGCTTCTT GGGGCCTCCG 
GCCCCCCGCT TTTCTGTGGG CACTCAGGAT TCGCACACCC CTCCCACTCC CCCAACCACC 
AGCAGCAGCA GCAGCAACAA TGGCAGCCAC AGCAGCAGCC CTACTGGGCC TGTGTCCTTC 
CCCCCACCTC CTTATCTGGC CAGAAGTATG GACTCCCTTC CCCGGCCCCC CAGCCCCACA 
CTGAGCCCCC AGGACCCACC CCTTGCACCC CTGACTCTTG CCCTGCCTTC AGCCCCTCCC 
TCCTCTTGCC ACCAAAATAC CTCAGGAAGC TTCAGGCGCC CGGAGAGCCC TCGGCCCAGG 
GTCTCCTTCC CAAAGACCCC CGAGGTGGGG CCGGGGCCAT CCCCAGGCCC CCTGAATAAA 
ACCCCCCAGC CTGTGCCGCC CAGGGTCGGG GAGCTGCCTG CCCGAGGCCC TCGACTTTTT 
GATTTCCCTC CTACCCCTCT GGAGGATCAG TTTGAGGAGC CAGCTGAATT CAAGATCCTA 
CCTGATGGGC TGGCTAATAT CATGAAGATG CTGGATGAAT CCATTCGCAA GGAGGAGGAG 
CAGCAACAAC AGGAGGCAGG CGCGGTCCCC GTCCCCCCGC CTCCTCTGAA GGAGCCCTTT 
GCATCTCTGC AGCCTCCGTT CCCCACAGAC ACAGCCCCAG CCACCACTGC TGCCGCCGCC 
GCCACCGCCA CCACCACCAC CACCACCACC ACAGCCACCC AGGAAGAGGA GAAGAAGCCA 
CCAGCCCTGC CACCACCGCC GCCTCTAGCC AAGTTCCCCC CACCACCCCA GCCACAGCCA 
CCGCCACCCC CGCTCCCCCC ACCAGCCAGC CCGGCCAGCC TGCTCAAGTC CTTGGCCTCC 
GTGCTGGAGG GACAAAAGTA CTGTTACCGA GGGACTGGAG CTGCTGTTCC CACCCGGCCT 
GGGTCCTTGC CCACCACTCA GTATTCCCCT GGTCCCTCAT CAGGTGCTAC CGCCCCGCCA 
CCCACCTCAG TGGCCCCGAG CGCCCAGGGC TCCCCACAGC CCTCCGCTTC CTCGTCATCT 
CAGTTCTCTA CCTCAGGCGG GCCCTGGGCC CGGGAGCGCA GGGCCAGCGA AGAGCCAGCC 
CCAGGCCCCA TGGCCCCCGC CCCGCCGCCC CCACCCCTGC CTCTGCCCCC TGCTCGCTCT 
GAGTCTGAGG TGCTAGAAGA GATCAGTCGG GCTTGTGAGA CCCTTGTGGA GCGGGTAGGC 
CGGAGTGCCA CGGACCCGGC AGACCTGGTG GACACAGCAG ATGCAGCGGA CAGTGGAGCT 
GAGCGATTGC TGCCTCCAGC TCCGGCCAAG GAGGAGAGCG TTGGGGTGGC AGCTGCGGCA 
GGACCCGGGG GCAGCAAGCG GCGGCAGAAG GAGCACCAGA AAGAGCACCG GCGGCACAGG 
CGGGCCTGCA AGGACAGTGT GGGGCGGCGG CCCCGTGAGG GCAGGGCCAA GACCAAGGCC 
AAGGCCCCCA AAGAAAAGAG CCGCAGGGTG CTGGGGAACC TGGACTTGCA GAGCGAGGAG 
ATCCAGGGTC GTGAGAAAGC CCGGCCAGAT CTTGGTGGGG CCTCCAAGGC CAAGCCACCC 
ACAGCTCCAG GCCCTCCACC TGCTCCTGCC CCCTCTGCCC AGCCCACACC CCCGGCAGCC 
CCTGCCCCTG GGAAGAAAGC TCGAGAGGAA GCTTCAGGGC CACCAGGGGT CAGCCGGGCT 
GACATGCTGA AGCTGCGCTC ACTCAGCGAA GGGCCCCCCA AGGAGCTGAA GATCCGGCTC 
ATCAAGGTAG AGAGCGGTGA CAAGGAGACC TTCATCGCCT CCGAGGTGGA AGAGCGGCGG 
CTGCGGATGG CAGACCTTAC CATCAGCCAC TGTGCTGCTG ACGTTGTGCG TGCCAGCAAG 
AACGCCAAGG TGAAAGGGAA GTTTCGAGAG TCCTACCTGT CCCCTGCCCA GTCTGTGAAA 
CCGAAGATCA ACACTGAGGA AAAGCTGCCC CGGGAAAAAC TCAACCCACC CACACCTAGC 
ATCTATCTGG AAAGCAAACG GGATGCCTTC TCGCCGGTGC TGTTACAGTT CTGTACAGAC 
CCTCGAAATC CCATCACCGT GATCCGGGGC CTGGCAGGCT CCCTGCGGCT CAACTTGGGC 
CTCTTCTCCA CCAAGACTCT GGTGGAGGCG AGTGGGGAGC ACACAGTGGA GGTGCGCACC 
CAGGTGCAGC AGCCCTCGGA TGAGAACTGG GATCTGACGG GCACACGACA GATCTGGCCC 
TGCGAGAGCT CCCGTTCCCA TACCACCATT GCCAAGTACG CGCAGTACCA GGCCTCATCC 
TTCCAGGAAT CCCTGCAGGA GGAGAAGGAG AGTGAGGACG AGGAGTCAGA GGAGCCGGAC 
AGCACCACAG GAACCCCTCC TAGCAGTGCA CCAGACCCGA AGAACCATCA CATCATCAAG 
TTTGGCACCA ACATCGACCT GTCTGATGCT AAGCGGTGGA AGCCCCAGCT GCAGGAGCTA 
CTGAAGCTGC CCGCCTTCAT GCGTGTCACC TCCACGGGGA ACATGCTGAG CCACGTGGGC 
CACACCATCC TGGGCATGAA CACGGTGCAG CTGTACATGA AGGTGCCCGG CAGCCGAACG 
CCAGGCCATC AAGAGAACAA CAACTTCTGC TCGGTCAACA TCAACATTGG CCCAGGAGAC 
TGCGAGTGGT TCGCGGTGCA CGAGCACTAC TGGGAGACCA TCAGCGCCTT CTGCGACCGG 
CACGGCGTGG ACTACCTGAC GGGTTCCTGG TGGCCAATCC TGGATGACCT CTATGCTTCC 
AACATCCCTG TGTACCGCTT CGTGCAGCGC CCCGGAGACC TGGTGTGGAT TAATGCGGGC 
ACCGTGCACT GGGTGCAGGC CACCGGCTGG TGTAACAACA TCGCCTGGAA CGTGGGGCCC 
CTCACCGCCT ATCAGTACCA GCTGGCCCTG GAACGATATG AGTGGAATGA GGTGAAGAAT 
GTCAAATCCA TTGTGCCCAT GATTCACGTG TCCTGGAACG TGGCTCGCAC GGTCAAAATC 
AGCGACCCCG ACTTGTTCAA GATGATCAAG TTCTGCCTCC TGCAGTCCAT GAAGCACTGC 
CAGGTGCAGC GGGAGAGCCT GGTGCGGGCC GGGAAGAAAA TTGCCTACCA GGGCCGGGTC 
AAGGATGAGC CCGCCTACTA CTGCAACGAG TGCGACGTGG AGGTGTTCAA CATCCTGTTC 
GTGACGAGCG AGAACGGCAG CCGCAACACG TACCTGGTGC ACTGCGAGGC GTGCGCGCGG 
CGCCGCAGCG CGGGCCTGCA GGGCGTGGTG GTGTTGGAGC AATACCGCAC CGAAGAGCTG 
GCGCAGGCCT ATGACGCCTT CACGCTGGCC CCCGCCAGCA CGTCGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015458745.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314231.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015458745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314231.1
CDS	416..5353
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015458745.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458745.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015458746.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314232.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015458746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314232.1
CDS	1359..6296
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015458746.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458746.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015458747.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314233.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015458747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314233.1
CDS	648..5585
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015458747.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458747.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015468042.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323528.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015468042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323528.1
CDS	648..5585
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015468042.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468042.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_010816061.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814363.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_010816061.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816061.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814363.1
CDS	991..5928
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_010816061.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816061.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_010816062.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814364.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_010816062.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816062.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814364.1
CDS	531..5468
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_010816062.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816062.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_010816063.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814365.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_010816063.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816063.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814365.1
CDS	412..5349
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_010816063.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816063.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_010816060.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814362.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_010816060.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814362.1
CDS	490..5427
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_010816060.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816060.2

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015468034.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323520.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015468034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323520.1
CDS	535..5472
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015468034.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468034.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015468037.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323523.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015468037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323523.1
CDS	991..5928
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015468037.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468037.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015468039.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323525.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015468039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323525.1
CDS	412..5349
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015468039.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468039.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015468036.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323522.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015468036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323522.1
CDS	461..5398
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015468036.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468036.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015468038.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323524.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015468038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323524.1
CDS	531..5468
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015468038.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468038.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015468035.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323521.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015468035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323521.1
CDS	490..5427
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015468035.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468035.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_010816059.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814361.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_010816059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814361.1
CDS	442..5379
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_010816059.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816059.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_003587412.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587460.2 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_003587412.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587412.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587460.2
CDS	535..5472
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_003587412.4
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587412.4

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015468040.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323526.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015468040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323526.1
CDS	416..5353
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015468040.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468040.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015468041.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323527.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.

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CloneID	OBa29804
Clone ID Related Accession (Same CDS sequence)	XM_015458745.1 , XM_015458746.1 , XM_015458747.1 , XM_015468042.1 , XM_010816061.2 , XM_010816062.2 , XM_010816063.2 , XM_010816060.2 , XM_015468034.1 , XM_015468037.1 , XM_015468039.1 , XM_015468036.1 , XM_015468038.1 , XM_015468035.1 , XM_010816059.1 , XM_003587412.4 , XM_015468040.1 , XM_015468041.1
Accession Version	XM_015468041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323527.1
CDS	1359..6296
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015468041.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468041.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG CAGTGCACCA 
GACCCGAAGA ACCATCACAT CATCAAGTTT GGCACCAACA TCGACCTGTC TGATGCTAAG 
CGGTGGAAGC CCCAGCTGCA GGAGCTACTG AAGCTGCCCG CCTTCATGCG TGTCACCTCC 
ACGGGGAACA TGCTGAGCCA CGTGGGCCAC ACCATCCTGG GCATGAACAC GGTGCAGCTG 
TACATGAAGG TGCCCGGCAG CCGAACGCCA GGCCATCAAG AGAACAACAA CTTCTGCTCG 
GTCAACATCA ACATTGGCCC AGGAGACTGC GAGTGGTTCG CGGTGCACGA GCACTACTGG 
GAGACCATCA GCGCCTTCTG CGACCGGCAC GGCGTGGACT ACCTGACGGG TTCCTGGTGG 
CCAATCCTGG ATGACCTCTA TGCTTCCAAC ATCCCTGTGT ACCGCTTCGT GCAGCGCCCC 
GGAGACCTGG TGTGGATTAA TGCGGGCACC GTGCACTGGG TGCAGGCCAC CGGCTGGTGT 
AACAACATCG CCTGGAACGT GGGGCCCCTC ACCGCCTATC AGTACCAGCT GGCCCTGGAA 
CGATATGAGT GGAATGAGGT GAAGAATGTC AAATCCATTG TGCCCATGAT TCACGTGTCC 
TGGAACGTGG CTCGCACGGT CAAAATCAGC GACCCCGACT TGTTCAAGAT GATCAAGTTC 
TGCCTCCTGC AGTCCATGAA GCACTGCCAG GTGCAGCGGG AGAGCCTGGT GCGGGCCGGG 
AAGAAAATTG CCTACCAGGG CCGGGTCAAG GATGAGCCCG CCTACTACTG CAACGAGTGC 
GACGTGGAGG TGTTCAACAT CCTGTTCGTG ACGAGCGAGA ACGGCAGCCG CAACACGTAC 
CTGGTGCACT GCGAGGCGTG CGCGCGGCGC CGCAGCGCGG GCCTGCAGGG CGTGGTGGTG 
TTGGAGCAAT ACCGCACCGA AGAGCTGGCG CAGGCCTATG ACGCCTTCAC GCTGGCCCCC 
GCCAGCACGT CGCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015468043.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323529.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.

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CloneID	OBa93888
Clone ID Related Accession (Same CDS sequence)	XM_015468043.1 , XM_015458748.1
Accession Version	XM_015468043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646455.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323529.1
CDS	535..5469
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015468043.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468043.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6B ( XM_015458748.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314234.1 Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.

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CloneID	OBa93888
Clone ID Related Accession (Same CDS sequence)	XM_015468043.1 , XM_015458748.1
Accession Version	XM_015458748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysine-specific demethylase 6B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314234.1
CDS	535..5469
Translation	MHRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASLGQPPLSAPLPPSHGSSSGHPNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAIRCYHSALRYGGSLAELGPRVSRLQQAQLWNFHAGSCQHRPKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRAAEPPVVQPVPPAALSGPSGEEGLSPGGKRRRGCSSEQTGLPPGLPLPPPPLPPPPPPPPPPPPPPPLPGLATSPPFQLTKPGLWSTLHGDAWGPERKGLAPPERQEQRHSLPHSYPYPAPAYPAHPPGHRLVPAAPPGPGPRPPGAESHGCPPATRPPGSDLRESRVQRSRMDSSVSPAATTACVPYAPSRPPGLPGTTSSSSSNTGPRGVEPSPGIPGADHYQTPALEVSSHQGRLGPSAHSSRKPFLAAPAATPHLSLPPGPTSPPPPSCPRLIRPPPPPAWLKGPACRAAREDGEILEELFFGAEGRPRPPPPPLPHREGFLGPPAPRFSVGTQDSHTPPTPPTTSSSSSNNGSHSSSPTGPVSFPPPPYLARSMDSLPRPPSPTLSPQDPPLAPLTLALPSAPPSSCHQNTSGSFRRPESPRPRVSFPKTPEVGPGPSPGPLNKTPQPVPPRVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANIMKMLDESIRKEEEQQQQEAGAVPVPPPPLKEPFASLQPPFPTDTAPATTAAAAATATTTTTTTTTATQEEEKKPPALPPPPPLAKFPPPPQPQPPPPPLPPPASPASLLKSLASVLEGQKYCYRGTGAAVPTRPGSLPTTQYSPGPSSGATAPPPTSVAPSAQGSPQPSASSSSQFSTSGGPWARERRASEEPAPGPMAPAPPPPPLPLPPARSESEVLEEISRACETLVERVGRSATDPADLVDTADAADSGAERLLPPAPAKEESVGVAAAAGPGGSKRRQKEHQKEHRRHRRACKDSVGRRPREGRAKTKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDLGGASKAKPPTAPGPPPAPAPSAQPTPPAAPAPGKKAREEASGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASEVEERRLRMADLTISHCAADVVRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEEKESEDEESEEPDSTTGTPPSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQSMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEACARRRSAGLQGVVVLEQYRTEELAQAYDAFTLAPASTSR

Target ORF information:

RefSeq Version	XM_015458748.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lysine (K)-specific demethylase 6B (KDM6B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458748.1

ORF Insert Sequence:

ATGCATCGGG CAGTGGACCC TCCAGGGGCC CGCGCTGCAC GGGAAGCCTT TGCCCTTGGG 
GGCTTGAGCT GTGCTGGGGC CTGGAGCTCC TGCCCGCCCC ATCCCCCTCC TCGGAGCGCA 
TGGCTGCCTG GAGGCAGGTG CTCTGCCAGC CTCGGGCAGC CCCCACTCTC TGCTCCCCTA 
CCCCCTTCAC ACGGCAGTAG CTCTGGGCAC CCCAACAAAC CGTATTATGC TCCAGGGACA 
CCCACCCCAA GACCCCTCCA TGGGAAGCTG GAGTCCCTGC ATGGCTGTGT GCAGGCATTG 
CTCCGGGAGC CAGCCCAGCC AGGGCTGTGG GAGCAGCTTG GGCAGCTGTA CGAGTCAGAG 
CATGACAGTG AGGAGGCTAT TCGCTGCTAC CACAGCGCCC TTCGATACGG AGGAAGCTTG 
GCTGAGTTGG GCCCCCGGGT CAGCCGACTC CAGCAGGCCC AGCTCTGGAA CTTTCATGCT 
GGGTCCTGCC AGCACCGACC CAAGGTCCTG CCCCCATTGG AGCAAGTGTG GAACTTGCTA 
CACCTTGAGC ACAAACGGAA CTACGGGGCC AAGCGGGGGG GTCCCCCAGT GAAACGAGCC 
GCTGAACCCC CAGTGGTGCA GCCTGTGCCT CCCGCAGCAC TCTCAGGCCC CTCAGGGGAG 
GAGGGGCTCA GCCCTGGTGG CAAGCGCAGG AGAGGCTGCA GCTCTGAGCA GACGGGCCTT 
CCTCCAGGGC TGCCACTGCC TCCACCACCA TTGCCGCCAC CACCCCCCCC ACCCCCACCA 
CCACCCCCTC CCCCACCTCT GCCTGGCCTA GCCACCAGCC CTCCATTTCA GCTGACCAAG 
CCAGGGCTGT GGAGTACCCT TCATGGAGAT GCCTGGGGCC CCGAGCGCAA GGGTTTAGCA 
CCCCCAGAGC GCCAGGAGCA GCGGCACTCG CTGCCTCACT CATATCCATA CCCGGCTCCT 
GCTTACCCTG CTCACCCCCC TGGCCACCGG CTGGTCCCGG CTGCTCCCCC AGGCCCAGGC 
CCCCGCCCCC CAGGAGCAGA GAGCCATGGC TGCCCGCCTG CCACCCGTCC CCCCGGAAGT 
GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT CCAGCGTTTC ACCAGCAGCA 
ACCACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCCG GCCTCCCCGG CACCACCAGC 
AGCAGCAGCA GCAACACTGG TCCCCGTGGC GTGGAGCCGA GCCCAGGCAT TCCCGGCGCT 
GACCATTACC AGACACCCGC GCTGGAGGTC TCCTCTCACC AAGGCCGCCT GGGGCCCTCG 
GCACACAGTA GTCGGAAACC CTTCCTGGCG GCTCCTGCTG CCACTCCCCA CCTGTCTCTG 
CCACCTGGCC CCACCTCACC TCCTCCACCC TCTTGTCCCC GCCTCATACG CCCCCCACCC 
CCTCCTGCCT GGCTGAAGGG CCCAGCCTGC CGGGCAGCTC GTGAAGATGG AGAGATCTTA 
GAGGAGCTCT TCTTCGGGGC TGAGGGACGC CCCCGCCCTC CCCCCCCCCC ACTTCCCCAC 
CGCGAGGGCT TCTTGGGGCC TCCGGCCCCC CGCTTTTCTG TGGGCACTCA GGATTCGCAC 
ACCCCTCCCA CTCCCCCAAC CACCAGCAGC AGCAGCAGCA ACAATGGCAG CCACAGCAGC 
AGCCCTACTG GGCCTGTGTC CTTCCCCCCA CCTCCTTATC TGGCCAGAAG TATGGACTCC 
CTTCCCCGGC CCCCCAGCCC CACACTGAGC CCCCAGGACC CACCCCTTGC ACCCCTGACT 
CTTGCCCTGC CTTCAGCCCC TCCCTCCTCT TGCCACCAAA ATACCTCAGG AAGCTTCAGG 
CGCCCGGAGA GCCCTCGGCC CAGGGTCTCC TTCCCAAAGA CCCCCGAGGT GGGGCCGGGG 
CCATCCCCAG GCCCCCTGAA TAAAACCCCC CAGCCTGTGC CGCCCAGGGT CGGGGAGCTG 
CCTGCCCGAG GCCCTCGACT TTTTGATTTC CCTCCTACCC CTCTGGAGGA TCAGTTTGAG 
GAGCCAGCTG AATTCAAGAT CCTACCTGAT GGGCTGGCTA ATATCATGAA GATGCTGGAT 
GAATCCATTC GCAAGGAGGA GGAGCAGCAA CAACAGGAGG CAGGCGCGGT CCCCGTCCCC 
CCGCCTCCTC TGAAGGAGCC CTTTGCATCT CTGCAGCCTC CGTTCCCCAC AGACACAGCC 
CCAGCCACCA CTGCTGCCGC CGCCGCCACC GCCACCACCA CCACCACCAC CACCACCACA 
GCCACCCAGG AAGAGGAGAA GAAGCCACCA GCCCTGCCAC CACCGCCACC TCTAGCCAAG 
TTCCCCCCAC CACCCCAGCC ACAGCCACCG CCACCCCCGC TCCCCCCACC AGCCAGCCCG 
GCCAGCCTGC TCAAGTCCTT GGCCTCCGTG CTGGAGGGAC AAAAGTACTG TTACCGAGGG 
ACTGGAGCTG CTGTTCCCAC CCGGCCTGGG TCCTTGCCCA CCACTCAGTA TTCCCCTGGT 
CCCTCATCAG GTGCTACCGC CCCGCCACCC ACCTCAGTGG CCCCGAGCGC CCAGGGCTCC 
CCACAGCCCT CCGCTTCCTC GTCATCTCAG TTCTCTACCT CAGGCGGGCC CTGGGCCCGG 
GAGCGCAGGG CCAGCGAAGA GCCAGCCCCA GGCCCCATGG CCCCCGCCCC GCCGCCCCCA 
CCCCTGCCTC TGCCCCCTGC TCGCTCTGAG TCTGAGGTGC TAGAAGAGAT CAGTCGGGCT 
TGTGAGACCC TTGTGGAGCG GGTAGGCCGG AGTGCCACGG ACCCGGCAGA CCTGGTGGAC 
ACAGCAGATG CAGCGGACAG TGGAGCTGAG CGATTGCTGC CTCCAGCTCC GGCCAAGGAG 
GAGAGCGTTG GGGTGGCAGC TGCGGCAGGA CCCGGGGGCA GCAAGCGGCG GCAGAAGGAG 
CACCAGAAAG AGCACCGGCG GCACAGGCGG GCCTGCAAGG ACAGTGTGGG GCGGCGGCCC 
CGTGAGGGCA GGGCCAAGAC CAAGGCCAAG GCCCCCAAAG AAAAGAGCCG CAGGGTGCTG 
GGGAACCTGG ACTTGCAGAG CGAGGAGATC CAGGGTCGTG AGAAAGCCCG GCCAGATCTT 
GGTGGGGCCT CCAAGGCCAA GCCACCCACA GCTCCAGGCC CTCCACCTGC TCCTGCCCCC 
TCTGCCCAGC CCACACCTCC GGCAGCCCCT GCCCCTGGGA AGAAAGCTCG AGAGGAAGCT 
TCAGGGCCAC CAGGGGTCAG CCGGGCTGAC ATGCTGAAGC TGCGCTCACT CAGCGAAGGG 
CCCCCCAAGG AGCTGAAGAT CCGGCTCATC AAGGTAGAGA GCGGTGACAA GGAGACCTTC 
ATCGCCTCCG AGGTGGAAGA GCGGCGGCTG CGGATGGCAG ACCTTACCAT CAGCCACTGT 
GCTGCTGACG TTGTGCGTGC CAGCAAGAAC GCCAAGGTGA AAGGGAAGTT TCGAGAGTCC 
TACCTGTCCC CTGCCCAGTC TGTGAAACCG AAGATCAACA CTGAGGAAAA GCTGCCCCGG 
GAAAAACTCA ACCCACCCAC ACCTAGCATC TATCTGGAAA GCAAACGGGA TGCCTTCTCG 
CCGGTGCTGT TACAGTTCTG TACAGACCCT CGAAATCCCA TCACCGTGAT CCGGGGCCTG 
GCAGGCTCCC TGCGGCTCAA CTTGGGCCTC TTCTCCACCA AGACTCTGGT GGAGGCGAGT 
GGGGAGCACA CAGTGGAGGT GCGCACCCAG GTGCAGCAGC CCTCGGATGA GAACTGGGAT 
CTGACGGGCA CACGACAGAT CTGGCCCTGC GAGAGCTCCC GTTCCCATAC CACCATCGCC 
AAGTACGCGC AGTACCAGGC CTCATCCTTC CAGGAATCCC TGCAGGAGGA GAAGGAGAGT 
GAGGACGAGG AGTCAGAGGA GCCGGACAGC ACCACAGGAA CCCCTCCTAG TGCACCAGAC 
CCGAAGAACC ATCACATCAT CAAGTTTGGC ACCAACATCG ACCTGTCTGA TGCTAAGCGG 
TGGAAGCCCC AGCTGCAGGA GCTACTGAAG CTGCCCGCCT TCATGCGTGT CACCTCCACG 
GGGAACATGC TGAGCCACGT GGGCCACACC ATCCTGGGCA TGAACACGGT GCAGCTGTAC 
ATGAAGGTGC CCGGCAGCCG AACGCCAGGC CATCAAGAGA ACAACAACTT CTGCTCGGTC 
AACATCAACA TTGGCCCAGG AGACTGCGAG TGGTTCGCGG TGCACGAGCA CTACTGGGAG 
ACCATCAGCG CCTTCTGCGA CCGGCACGGC GTGGACTACC TGACGGGTTC CTGGTGGCCA 
ATCCTGGATG ACCTCTATGC TTCCAACATC CCTGTGTACC GCTTCGTGCA GCGCCCCGGA 
GACCTGGTGT GGATTAATGC GGGCACCGTG CACTGGGTGC AGGCCACCGG CTGGTGTAAC 
AACATCGCCT GGAACGTGGG GCCCCTCACC GCCTATCAGT ACCAGCTGGC CCTGGAACGA 
TATGAGTGGA ATGAGGTGAA GAATGTCAAA TCCATTGTGC CCATGATTCA CGTGTCCTGG 
AACGTGGCTC GCACGGTCAA AATCAGCGAC CCCGACTTGT TCAAGATGAT CAAGTTCTGC 
CTCCTGCAGT CCATGAAGCA CTGCCAGGTG CAGCGGGAGA GCCTGGTGCG GGCCGGGAAG 
AAAATTGCCT ACCAGGGCCG GGTCAAGGAT GAGCCCGCCT ACTACTGCAA CGAGTGCGAC 
GTGGAGGTGT TCAACATCCT GTTCGTGACG AGCGAGAACG GCAGCCGCAA CACGTACCTG 
GTGCACTGCG AGGCGTGCGC GCGGCGCCGC AGCGCGGGCC TGCAGGGCGT GGTGGTGTTG 
GAGCAATACC GCACCGAAGA GCTGGCGCAG GCCTATGACG CCTTCACGCT GGCCCCCGCC 
AGCACGTCGC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KDM6B
Entrez Gene ID	100140276
Full Name	lysine demethylase 6B
General protein information	Preferred Names lysine demethylase 6B Names lysine-specific demethylase 6B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Mus musculus (house mouse)	Kdm6b	NP_001017426.1
Pan troglodytes (chimpanzee)	KDM6B	XP_003315411.1
Rattus norvegicus (Norway rat)	Kdm6b	NP_001102299.1
Canis lupus familiaris (dog)	KDM6B	XP_005620054.1
Bos taurus (cattle)	KDM6B	XP_003587460.2
Homo sapiens (human)	KDM6B	NP_001073893.1
	KDM6B	XP_001110616.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KDM6B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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