LOC100139548 cDNA ORF clone, Bos taurus(cattle)

The following LOC100139548 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100139548 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa11261 XM_003587695.5
Latest version!
Bos taurus helicase SKI2W (LOC100139548), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa11261 XM_003584612.4
Latest version!
Bos taurus helicase SKI2W (LOC100139548), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa11261 XM_003587695.4 Bos taurus helicase SKI2W (LOC100139548), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa11261
    Clone ID Related Accession (Same CDS sequence) XM_003584612.4 , XM_003587695.4 , XM_003587695.5
    Accession Version XM_003584612.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product helicase SKI2W
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646599.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003584612.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
    GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG
    AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG
    TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA
    TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC
    GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG
    CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC
    ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT
    GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG
    AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT
    CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG
    GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC
    ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA
    GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG
    GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT
    CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG
    CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT
    GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG
    CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG
    GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG
    GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT
    ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA
    CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC
    CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT
    GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG
    GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG
    GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG
    CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC
    CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC
    GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT
    CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG
    GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG
    CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT
    GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC
    ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG
    GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG
    GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG
    CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC
    AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG
    GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG
    GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG
    ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA
    GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT
    CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC
    GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC
    CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC
    CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC
    TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC
    GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC
    ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC
    CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC
    CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG
    CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG
    GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG
    GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG
    AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC
    GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT
    GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA
    TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG
    CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC
    CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT
    GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT
    GCCAGCCTCT ACACCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003584660.2
    CDS96..3836
    Translation

    Target ORF information:

    RefSeq Version XM_003584612.4
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase SKI2W (LOC100139548), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003584612.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
    GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG
    AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG
    TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA
    TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC
    GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG
    CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC
    ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT
    GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG
    AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT
    CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG
    GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC
    ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA
    GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG
    GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT
    CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG
    CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT
    GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG
    CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG
    GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG
    GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT
    ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA
    CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC
    CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT
    GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG
    GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG
    GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG
    CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC
    CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC
    GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT
    CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG
    GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG
    CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT
    GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC
    ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG
    GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG
    GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG
    CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC
    AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG
    GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG
    GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG
    ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA
    GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT
    CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC
    GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC
    CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC
    CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC
    TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC
    GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC
    ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC
    CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC
    CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG
    CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG
    GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG
    GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG
    AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC
    GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT
    GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA
    TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG
    CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC
    CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT
    GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT
    GCCAGCCTCT ACACCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11261
    Clone ID Related Accession (Same CDS sequence) XM_003584612.4 , XM_003587695.4 , XM_003587695.5
    Accession Version XM_003587695.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product helicase SKI2W
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587695.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
    GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG
    AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG
    TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA
    TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC
    GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG
    CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC
    ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT
    GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG
    AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT
    CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG
    GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC
    ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA
    GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG
    GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT
    CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG
    CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT
    GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG
    CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG
    GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG
    GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT
    ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA
    CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC
    CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT
    GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG
    GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG
    GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG
    CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC
    CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC
    GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT
    CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG
    GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG
    CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT
    GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC
    ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG
    GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG
    GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG
    CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC
    AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG
    GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG
    GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG
    ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA
    GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT
    CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC
    GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC
    CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC
    CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC
    TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC
    GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC
    ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC
    CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC
    CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG
    CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG
    GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG
    GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG
    AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC
    GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT
    GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA
    TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG
    CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC
    CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT
    GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT
    GCCAGCCTCT ACACCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587743.1
    CDS96..3836
    Translation

    Target ORF information:

    RefSeq Version XM_003587695.4
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase SKI2W (LOC100139548), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587695.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
    GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG
    AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG
    TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA
    TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC
    GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG
    CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC
    ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT
    GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG
    AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT
    CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG
    GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC
    ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA
    GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG
    GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT
    CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG
    CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT
    GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG
    CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG
    GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG
    GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT
    ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA
    CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC
    CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT
    GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG
    GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG
    GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG
    CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC
    CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC
    GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT
    CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG
    GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG
    CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT
    GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC
    ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG
    GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG
    GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG
    CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC
    AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG
    GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG
    GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG
    ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA
    GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT
    CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC
    GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC
    CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC
    CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC
    TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC
    GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC
    ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC
    CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC
    CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG
    CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG
    GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG
    GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG
    AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC
    GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT
    GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA
    TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG
    CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC
    CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT
    GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT
    GCCAGCCTCT ACACCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa11261
    Clone ID Related Accession (Same CDS sequence) XM_003584612.4 , XM_003587695.4 , XM_003587695.5
    Accession Version XM_003587695.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product helicase SKI2W
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587695.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
    GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG
    AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG
    TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA
    TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC
    GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG
    CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC
    ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT
    GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG
    AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT
    CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG
    GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC
    ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA
    GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG
    GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT
    CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG
    CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT
    GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG
    CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG
    GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG
    GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT
    ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA
    CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC
    CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT
    GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG
    GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG
    GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG
    CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC
    CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC
    GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT
    CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG
    GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG
    CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT
    GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC
    ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG
    GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG
    GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG
    CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC
    AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG
    GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG
    GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG
    ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA
    GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT
    CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC
    GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC
    CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC
    CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC
    TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC
    GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC
    ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC
    CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC
    CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG
    CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG
    GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG
    GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG
    AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC
    GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT
    GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA
    TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG
    CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC
    CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT
    GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT
    GCCAGCCTCT ACACCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587743.1
    CDS98..3838
    Translation

    Target ORF information:

    RefSeq Version XM_003587695.5
    Organism Bos taurus(cattle)
    Definition Bos taurus helicase SKI2W (LOC100139548), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587695.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATGGAGA CGGAGCGACT CGTGCTACCT CCCCCAGATC CCCTGGACCT GCCCCTTCGG 
    GCCGTAGAGC TGGGATGCAC TGGGCGCTGG GAGTTGCTGA ATGTGCCTGG GGCTCCAGAG
    AGCACCCTTC CCCACGGCCT CCCTCCCTGT GCCCCAGATC TGCAGCAGGA AGCAGAGCGG
    TTGTTTCTGT CATCTCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGGGAA
    TGGCAGAGGA AGATGGATCC TTGGTCTCTC CTGGCCACAC TGGGGGCCCC AGTCCCCTCC
    GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TAGGCTACAA GGAGGTCCTG
    CTAGAGAATA CAAACCTGTC GTCCACAACC TCCTTGTCTC TTCGCCGGCC ACCAGCGCCC
    ATCTCCCAGT CCCTGTGGGG GAATCCAACA CAGTACCCTT TCTGGCCAGG TGGAATGGAT
    GAGCCCAGTA TAACAGATCT GAGCACTCGT GAGGAGGCTG AGGAGGAGAT AGACTTTGAG
    AAAGACCTCC TTACTCTTCC ACCTGGCTTC AAGAAAGGCG TGGACTTCGC ACCAAAGGGT
    CACTCAGCTC CAGCTGCCGG GTTGCTCAGC CTCAGCCGTC TGCTGGAGCC CTTGGATTTG
    GGTGGGGGCG ATGAGGATGA GAGTGAGGCA GTGGGACAGC CTGGCGGGCC CAGACAGGAC
    ACTGTTTCAG CCTCTCCTGG CAGTGTTCCT CTGGCCCGAG CAAGCAGCTT GGAGGACCTA
    GTGTTGAAGG AAGCGGCCAC AACTGTAGCC CCCCCAGAAC CTCCCAAGCC CCCACCTCAG
    GAGCAGTGGG CCATTCCTGT GGATGTTACC TCCCCTGTTG GTGATTTCTA CCGCCTCATT
    CCCCAGCCAG CCTTTCAGTG GGCCTTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG
    CACTTGGAGC GGCACGACTC AGTCTTCGTC GCGGCTCACA CATCCGCGGG GAAGACGGTT
    GTGGCTGAAT ATGCCATCGC GCTTGCCCAG AAACACATGA CGCGCACCAT CTATACTTCG
    CCCATCAAGG CTCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACGTT TGGGGACGTG
    GGGCTGCTCA CCGGGGATGT GCAGCTGCAC CCAGAAGCCT CGTGCCTCAT TATGACAACG
    GAGATCCTTC GCTCCATGCT GTACAGTGGC TCGGACGTCA TCCGGGACCT GGAGTGGGTT
    ATCTTTGATG AGGTTCACTA TATCAATGAT GCTGAGCGCG GGGTCGTGTG GGAGGAGGTA
    CTCATCATGC TGCCTGACCA CGTGTCCATC ATCCTTCTGA GTGCTACTGT CCCCAATGCC
    CTTGAGTTTG CTGACTGGAT TGGACGGCTG AAGCGTCGCC AGATCTACGT GATCAGCACT
    GTCGCTCGCC CAGTGCCCCT GGAGCACTAT CTCTTCACGG GGAACAGCCC CAAGACCCAG
    GGGGAGCTCT TCCTGTTGCT GGACTCCCGT GGCGCCTTCC ACACAAAGGG GTACTATGCG
    GCTGTGGAGG CCAAGAAGGA GCGGATGAGC AAGCATGCCC AGACCTTTGG GGCCAAGCAG
    CCCACGCATC AGGGGGGGCC TGCCCAGGAC CGCGGTGTGT ACCTGTCCCT CCTGGCCTCC
    CTCCGCACCC GTGCACAGCT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAC
    GAGCAGGCCT CGGGCCTCAC CTCCCTCGAC CTGACAACAA GTTCAGAGAA GAGTGAGATT
    CACCTCTTCC TACAGCGCTG CCTTGCCCGT CTCCGCGGCT CTGACCGCCA GCTGCCCCAG
    GTTCTGCACA TGTCGGAGCT CCTGCACCGT GGCCTGGGTG TGCACCACAG CGGCATCCTG
    CCCATCCTCA AGGAGATTGT GGAGATGCTC TTCAGCCGCG GCCTGGTCAA GGTCTTGTTT
    GCCACGGAGA CCTTCGCCAT GGGTGTAAAT ATGCCGGCCC GGACAGTGGT ATTTGACTCC
    ATGCGCAAGC ACGATGGCTC CACCTTCCGG GACCTACTCC CTGGGGAGTA TGTGCAGATG
    GCGGGCCGGG CGGGCCGGAG GGGCCTGGAC CCCACAGGCA CGGTCATCCT GCTCTGCAAG
    GGCCGCGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC ATCCCAGCTG
    CAGTCCCAGT TCCGCCTCAC GTACACCATG ATCCTCAACT TGCTGCGGGT GGATGCTCTC
    AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCAGAGTTTC CATCTCGCAA GGACAGCAAG
    GCCCATGAAC AGGCTCTGGC TGAACTGACC AAGAAGTTGG GAGCTTTGGA AGAGCCCGAG
    GTGACTGGCC AGCTCGTCGA CTTGCCTGAG TACTACAGCT GGGGGGAGGA ACTGACTGAG
    ACCCGGAGCC AGATCCAGCA CCGCATCATA GAGTCTGTGA ATGGGCTGAA ATCTCTCTCA
    GCGGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCACTGGG TGTGATCCTT
    CAGGTCTCTT CGAACTCCAC CAGCAGAGTA TTCACAGCCC TGGTCTTGTG TGACAAGCCC
    GTGTCTGAGG ACCCACGGGA GAGGGGGCCA GCCTCCCCAG ATGTGCCCTA CCCAGACGAC
    CTCGTGGGAT TCAAGCTGTT CCTGCCTGAG GGGCCCTGTG ACCACACTGT GGCCAAGCTC
    CAGCCAGGAG ACGTGGCCGC CATCACCACC AAGGTGCTCC GGCTAAATGG GGACAAGATC
    TTGGAGGACT TCAGCAAGCG GCAGCAACCA AAATTCAAGA AGGATCCGCC CTCTGCGGCC
    GTGACCACTG CTGTCCAGGA ACTGCTACGA CTGGCTCAGG CGCACCCCAC AGGACCCCCC
    ACCCTCGACC CTGTCAATGA CCTGCAACTC AAGGATGTGT CTGTGGTCGA GGGGGGGCTC
    CGGGCCCGGA AGCTGGAGGA GCTGATCCGG GGGGCTCAGT GTGTGCACAG TCCCCGTTTC
    CCTGCCCAGT ACCTGAAGCT GCGGGAGCGG ATGCAGATCC AGAAGGAGAT GGAGCGGCTG
    CGCTTCCTGC TGTCGGACCA GTCGCTGCTG CTGCTCCCGG AGTACCACCA GCGAGTAGAG
    GTGCTGCGAA CCCTGGGGTA TGTGGACGAG GCAGGCACAG TGAAGCTGGC CGGGCGGGTG
    GCTTGTGCCA TGAGCAGCCA CGAGCTGCTC CTCACCGAGC TCATGTTCGA CAACGCACTG
    AGCACCCTGC GGCCTGAGGA GATCGCTGCC CTGCTCTCCG GCCTGGTCTG CCAGAGCCCC
    GGGGACCCTG GTGATCAGCT CCCCAGCACC CTCAAACAGG GGGTGGAACG GGTCCGGACT
    GTGGCCAAGC GGATTGGTGA GGTCCAGGCG GCCTGTGGTT TGAACCAGAC TGTGGAGGAA
    TTTGTTGGGG AGCTGAATTT CGGGCTGGTT GAGGTTGTGT ACGAGTGGGC CCGGGGCATG
    CCCTTCTCTG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATC
    CAACGCCTGG CCGAGATGTG TCGCTCGCTT CGGGGGGCAG CCCGCCTGGT GGGCGAACCT
    GTGCTAGGTG CCAAGATGGA GACGGCAGCC ACCCTGTTAC GGAGGGACAT CGTCTTTGCT
    GCCAGCCTCT ACACCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.