SKIV2L cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SKIV2L
Entrez Gene ID	6499
Full Name	Ski2 like RNA helicase
Synonyms	170A,DDX13,HLP,SKI2,SKI2W,SKIV2,SKIV2L1,THES2
General protein information	Preferred Names Ski2 like RNA helicase Names helicase SKI2W SKI2 homolog, superkiller viralicidic activity 2-like helicase-like protein superkiller viralicidic activity 2-like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.33
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a human homologue of yeast SKI2 and may be involved in antiviral activity by blocking translation of poly(A) deficient mRNAs. This gene is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008].
Disorder MIM:	600478
Disorder Html:	Trichohepatoenteric syndrome 2, 614602 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011514815.3	XP_011513117.1	helicase SKI2W isoform X1
NM_006929.4	NP_008860.4	helicase SKI2W
XM_011514815.2	XP_011513117.1	helicase SKI2W isoform X1
NM_006929.5	NP_008860.4	helicase SKI2W

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003182	XP_312880.4
Homo sapiens (human)	SKIV2L	NP_008860.4
Danio rerio (zebrafish)	skiv2l	NP_001038366.1
Schizosaccharomyces pombe (fission yeast)	SPCC550.03c	NP_588095.1
Canis lupus familiaris (dog)	SKIV2L	XP_538841.2
Bos taurus (cattle)	SKIV2L	XP_003587743.1
Arabidopsis thaliana (thale cress)	AT3G46960	NP_190280.5
Pan troglodytes (chimpanzee)	SKIV2L	XP_001160927.2
	SKIV2L	XP_001106475.2
Rattus norvegicus (Norway rat)	Skiv2l	NP_998724.1
Drosophila melanogaster (fruit fly)	tst	NP_524465.1
Caenorhabditis elegans (roundworm)	skih-2	NP_502084.2
Saccharomyces cerevisiae (baker's yeast)	SKI2	NP_013502.3
Xenopus tropicalis (tropical clawed frog)	skiv2l	XP_004917184.1
Mus musculus (house mouse)	Skiv2l	NP_067312.2
Eremothecium gossypii	AGOS_AER067C	NP_984927.2
Neurospora crassa	NCU01857	XP_965488.1

Gene Ontology
Bibliography

Related articles in PubMed

Association between SKIV2L polymorphism rs429608 and age-related macular degeneration: A meta-analysis.
Shuai P, Ye Z, Liu Y, Qu C, Liu X, Luo H, Feng X, Li X, Shi Y, Gong B
Ophthalmic genetics38(3)245-251(2017 May-Jun)

Targeted next-generation sequencing identification of a novel missense mutation of the SKIV2L gene in a patient with trichohepatoenteric syndrome.
Zheng B, Pan J, Jin Y, Wang C, Liu Z
Molecular medicine reports14(3)2107-10(2016 Sep)

Novel mutations in SKIV2L and TTC37 genes in Malaysian children with trichohepatoenteric syndrome.
Lee WS, Teo KM, Ng RT, Chong SY, Kee BP, Chua KH
Gene586(1)1-6(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This meta-analysis showed that SKIV2L rs429608 was statistically associated with age-related macular degeneration(AMD) and it might exert a protective effect on AMD. Further investigations are needed to validate the association and confirm the role of SKIV2L in AMD.
Title: Association between SKIV2L polymorphism rs429608 and age-related macular degeneration: A meta-analysis.

The present study was the first, to the best of our knowledge, to report a case of a boy with THES resulting from compound heterozygous mutations of the SKIV2L gene in China.
Title: Targeted next-generation sequencing identification of a novel missense mutation of the SKIV2L gene in a patient with trichohepatoenteric syndrome.

Study reported three point mutations, which have not been previously described in other patients with THES in SKIV2L and TTC37 genes, including one nonsense, one frameshift, and one missense mutations.
Title: Novel mutations in SKIV2L and TTC37 genes in Malaysian children with trichohepatoenteric syndrome.

Data from 4 consanguineous families in Saudi Arabia suggest SKIV2L mutations in tricho-hepato-enteric syndrome can include deletions (c.3559_3579del, p.1187_1193del; 4 subjects) and nonsense mutation (c.C4102T, p.Q1368X; 1 subject). [CASE REPORT]
Title: Expanding phenotypic and allelic heterogeneity of tricho-hepato-enteric syndrome.

A SKIV2L variant was associated with protection against exudative age-related macular degeneration regardless of subtypes in the Japanese population.
Title: Genetic variants in the SKIV2L gene in exudative age-related macular degeneration in the Japanese population.

The following SKIV2L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SKIV2L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25855	NM_006929.5 Latest version!	Homo sapiens Ski2 like RNA helicase (SKIV2L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu60526	XM_011514815.3 Latest version!	Homo sapiens Ski2 like RNA helicase (SKIV2L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu60526	XM_011514815.2	Homo sapiens Ski2 like RNA helicase (SKIV2L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu25855	NM_006929.4	Homo sapiens Ski2 like RNA helicase (SKIV2L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SKIV2L ( NM_006929.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_008860.4 Homo sapiens Ski2 like RNA helicase (SKIV2L), mRNA.

Price & Availability↑

CloneID	OHu25855
Clone ID Related Accession (Same CDS sequence)	NM_006929.4 , NM_006929.5
Accession Version	NM_006929.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-19
Organism	Homo sapiens(human)
Product	helicase SKI2W
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645922.12, U09877.1, AL529319.3, BG396326.1, BE395851.1 and Z48796.1. This sequence is a reference standard in the RefSeqGene project. On Jun 15, 2005 this sequence version replaced NM_006929.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U09877.1, Z48796.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATGGAGA CAGAGCGACT TGTGCTACCC CCTCCAGATC CCCTGGACCT ACCCCTTCGG 
GCCGTGGAGC TCGGATGCAC GGGGCACTGG GAGCTGCTGA ACTTGCCTGG AGCTCCAGAG 
AGTAGCCTTC CCCATGGCCT CCCTCCTTGT GCCCCAGATC TGCAGCAAGA AGCAGAACAG 
TTGTTTCTGT CATCCCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGAAAA 
TGGCAGAGGA AGACGGATCC CTGGTCTCTT TTGGCTGTCC TGGGAGCCCC AGTCCCATCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TGGGTTACAA AGAGGTCTTG 
CTGGAGAACA CAAATCTCTC GGCTACAACC TCCTTGTCTC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CCTTATGGGG AAATCCAACT CAGTATCCCT TCTGGCCAGG GGGGATGGAT 
GAACCCACCA TAACAGATCT GAACACACGG GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TTACTATTCC ACCTGGTTTC AAGAAAGGCA TGGACTTTGC ACCAAAAGAT 
TGTCCAACTC CAGCTCCTGG ACTACTAAGC CTTAGCTGTA TGTTGGAGCC TCTGGATTTG 
GGTGGGGGTG ACGAGGATGA GAATGAGGCA GTGGGACAGC CAGGAGGTCC CAGAGGGGAC 
ACTGTTTCAG CCTCTCCCTG CAGTGCTCCC CTGGCCCGAG CAAGCAGCTT GGAAGACCTA 
GTGTTGAAGG AAGCGTCCAC AGCTGTATCC ACCCCAGAGG CCCCAGAGCC TCCATCTCAG 
GAGCAGTGGG CCATCCCTGT GGACGCCACC TCCCCTGTTG GTGATTTCTA TCGCCTCATT 
CCCCAGCCAG CCTTCCAGTG GGCATTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAAC GGCATGACTC TGTCTTTGTC GCAGCTCACA CATCTGCAGG AAAAACAGTT 
GTGGCTGAAT ATGCCATTGC CCTGGCCCAG AAACACATGA CACGCACCAT CTACACTTCG 
CCCATCAAGG CCCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACATT CGGGGATGTG 
GGGCTGCTCA CCGGGGATGT ACAGCTGCAT CCGGAGGCCT CCTGCCTCAT CATGACCACA 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCAGATGTTA TTCGGGACCT GGAGTGGGTC 
ATCTTTGATG AGGTTCACTA TATCAACGAT GTCGAGCGTG GGGTCGTGTG GGAGGAGGTG 
CTTATCATGC TACCTGACCA CGTTTCTATC ATCCTTCTGA GTGCCACCGT CCCCAACGCC 
CTTGAGTTTG CTGACTGGAT TGGGCGGCTG AAGCGTCGTC AGATCTATGT GATTAGCACT 
GTAACCCGCC CCGTGCCCCT GGAGCACTAT CTTTTCACAG GGAACAGCTC CAAGACCCAG 
GGGGAGCTCT TTTTGTTGCT GGACTCCCGA GGAGCCTTCC ATACAAAAGG GTACTATGCA 
GCTGTGGAGG CCAAGAAGGA GAGAATGAGC AAACACGCCC AGACCTTTGG GGCCAAGCAG 
CCCACACATC AGGGGGGCCC TGCACAGGAC CGCGGAGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACAC GTGCCCAGTT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAT 
GAGCAGGCCT CAGGCCTCAC CTCCCTTGAC CTCACCACCA GTTCGGAGAA GAGCGAGATC 
CACCTCTTCC TGCAGCGCTG CCTTGCTCGC CTCCGTGGCT CTGACCGCCA GCTGCCCCAG 
GTCCTGCACA TGTCAGAGCT CCTGAATCGC GGCCTGGGTG TGCACCATAG CGGCATCCTG 
CCCATCCTCA AGGAGATCGT GGAGATGCTC TTCAGCCGTG GCCTGGTCAA GGTCTTGTTT 
GCCACAGAGA CCTTTGCCAT GGGAGTAAAC ATGCCTGCTC GTACAGTAGT GTTTGACTCC 
ATGCGCAAAC ACGATGGCTC CACCTTCCGG GACCTGCTCC CTGGGGAGTA TGTGCAGATG 
GCAGGCCGGG CAGGGCGGAG GGGCCTGGAC CCCACAGGCA CCGTTATCCT GCTCTGCAAG 
GGCCGAGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC GTCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACTATG ATCCTCAACT TGCTGCGAGT GGATGCCCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCTGAGTTTC CCTCCCGCAA AGACAGCAAG 
GCCCATGAAC AGGCCCTGGC TGAACTGACC AAGAGGCTGG GAGCTTTGGA GGAGCCTGAC 
ATGACTGGCC AACTGGTCGA CCTGCCTGAA TATTACAGCT GGGGGGAGGA ACTGACAGAG 
ACCCAGCACA TGATCCAGCG ACGCATCATG GAGTCTGTGA ACGGGCTGAA GTCTCTCTCA 
GCAGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCATTGGG AGTGATCCTA 
CAGGTCTCCT CGAACTCCAC CAGCAGAGTA TTCACAACCC TGGTCTTGTG TGATAAGCCC 
TTGTCCCAGG ACCCACAGGA CAGGGGGCCA GCCACTGCAG AGGTGCCCTA TCCAGATGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAA GGGCCTTGTG ACCACACCGT GGTCAAGCTC 
CAGCCAGGAG ATATGGCTGC CATCACCACC AAGGTGCTCC GGGTGAATGG GGAGAAGATC 
TTGGAGGACT TCAGCAAGAG GCAGCAGCCA AAATTCAAGA AGGATCCTCC CCTTGCAGCC 
GTGACCACTG CTGTCCAGGA ACTGCTGCGT CTGGCTCAGG CCCACCCAGC CGGACCTCCC 
ACCCTCGACC CTGTCAATGA CCTGCAGCTC AAAGATATGT CAGTTGTAGA GGGTGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCAG GGGGCTCAGT GTGTACACAG CCCCCGTTTT 
CCTGCCCAGT ACCTGAAGCT GCGGGAGCGA ATGCAGATAC AGAAGGAGAT GGAGCGGCTG 
CGCTTCCTAC TGTCGGATCA GTCATTGCTG CTGCTTCCTG AGTACCATCA GCGAGTAGAG 
GTGCTCCGAA CCCTGGGTTA TGTGGACGAG GCGGGCACTG TGAAGCTGGC AGGGCGGGTG 
GCTTGTGCCA TGAGCAGCCA TGAGTTGCTC CTCACTGAGC TCATGTTTGA CAATGCACTG 
AGCACCCTGC GGCCTGAGGA GATTGCTGCC TTGCTCTCTG GCCTGGTCTG CCAGAGCCCT 
GGGGACGCTG GGGATCAGCT CCCAAACACC CTCAAGCAGG GAATAGAACG TGTCCGGGCT 
GTGGCCAAGC GGATTGGTGA GGTCCAGGTG GCTTGTGGCC TGAACCAGAC GGTGGAGGAA 
TTTGTGGGGG AGCTGAATTT TGGGCTGGTT GAGGTTGTAT ATGAGTGGGC CCGGGGCATG 
CCCTTCTCCG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATT 
CAGCGCCTGG CTGAGATGTG TCGCTCACTG CGGGGGGCAG CCCGCCTGGT AGGAGAGCCT 
GTGCTGGGTG CCAAGATGGA GACAGCGGCT ACCTTGCTAC GGCGGGACAT CGTATTTGCG 
GCCAGCCTCT ACACCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008860.4
CDS	390..4130
Translation	MMETERLVLPPPDPLDLPLRAVELGCTGHWELLNLPGAPESSLPHGLPPCAPDLQQEAEQLFLSSPAWLPLHGVEHSARKWQRKTDPWSLLAVLGAPVPSDLQAQRHPTTGQILGYKEVLLENTNLSATTSLSLRRPPGPASQSLWGNPTQYPFWPGGMDEPTITDLNTREEAEEEIDFEKDLLTIPPGFKKGMDFAPKDCPTPAPGLLSLSCMLEPLDLGGGDEDENEAVGQPGGPRGDTVSASPCSAPLARASSLEDLVLKEASTAVSTPEAPEPPSQEQWAIPVDATSPVGDFYRLIPQPAFQWAFEPDVFQKQAILHLERHDSVFVAAHTSAGKTVVAEYAIALAQKHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPEASCLIMTTEILRSMLYSGSDVIRDLEWVIFDEVHYINDVERGVVWEEVLIMLPDHVSIILLSATVPNALEFADWIGRLKRRQIYVISTVTRPVPLEHYLFTGNSSKTQGELFLLLDSRGAFHTKGYYAAVEAKKERMSKHAQTFGAKQPTHQGGPAQDRGVYLSLLASLRTRAQLPVVVFTFSRGRCDEQASGLTSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHMSELLNRGLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMRKHDGSTFRDLLPGEYVQMAGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMMGKPSQLQSQFRLTYTMILNLLRVDALRVEDMMKRSFSEFPSRKDSKAHEQALAELTKRLGALEEPDMTGQLVDLPEYYSWGEELTETQHMIQRRIMESVNGLKSLSAGRVVVVKNQEHHNALGVILQVSSNSTSRVFTTLVLCDKPLSQDPQDRGPATAEVPYPDDLVGFKLFLPEGPCDHTVVKLQPGDMAAITTKVLRVNGEKILEDFSKRQQPKFKKDPPLAAVTTAVQELLRLAQAHPAGPPTLDPVNDLQLKDMSVVEGGLRARKLEELIQGAQCVHSPRFPAQYLKLRERMQIQKEMERLRFLLSDQSLLLLPEYHQRVEVLRTLGYVDEAGTVKLAGRVACAMSSHELLLTELMFDNALSTLRPEEIAALLSGLVCQSPGDAGDQLPNTLKQGIERVRAVAKRIGEVQVACGLNQTVEEFVGELNFGLVEVVYEWARGMPFSELAGLSGTPEGLVVRCIQRLAEMCRSLRGAARLVGEPVLGAKMETAATLLRRDIVFAASLYTQ

Target ORF information:

RefSeq Version	NM_006929.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Ski2 like RNA helicase (SKIV2L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006929.4

ORF Insert Sequence:

ATGATGGAGA CAGAGCGACT TGTGCTACCC CCTCCAGATC CCCTGGACCT ACCCCTTCGG 
GCCGTGGAGC TCGGATGCAC GGGGCACTGG GAGCTGCTGA ACTTGCCTGG AGCTCCAGAG 
AGTAGCCTTC CCCATGGCCT CCCTCCTTGT GCCCCAGATC TGCAGCAAGA AGCAGAACAG 
TTGTTTCTGT CATCCCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGAAAA 
TGGCAGAGGA AGACGGATCC CTGGTCTCTT TTGGCTGTCC TGGGAGCCCC AGTCCCATCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TGGGTTACAA AGAGGTCTTG 
CTGGAGAACA CAAATCTCTC GGCTACAACC TCCTTGTCTC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CCTTATGGGG AAATCCAACT CAGTATCCCT TCTGGCCAGG GGGGATGGAT 
GAACCCACCA TAACAGATCT GAACACACGG GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TTACTATTCC ACCTGGTTTC AAGAAAGGCA TGGACTTTGC ACCAAAAGAT 
TGTCCAACTC CAGCTCCTGG ACTACTAAGC CTTAGCTGTA TGTTGGAGCC TCTGGATTTG 
GGTGGGGGTG ACGAGGATGA GAATGAGGCA GTGGGACAGC CAGGAGGTCC CAGAGGGGAC 
ACTGTTTCAG CCTCTCCCTG CAGTGCTCCC CTGGCCCGAG CAAGCAGCTT GGAAGACCTA 
GTGTTGAAGG AAGCGTCCAC AGCTGTATCC ACCCCAGAGG CCCCAGAGCC TCCATCTCAG 
GAGCAGTGGG CCATCCCTGT GGACGCCACC TCCCCTGTTG GTGATTTCTA TCGCCTCATT 
CCCCAGCCAG CCTTCCAGTG GGCATTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAAC GGCATGACTC TGTCTTTGTC GCAGCTCACA CATCTGCAGG AAAAACAGTT 
GTGGCTGAAT ATGCCATTGC CCTGGCCCAG AAACACATGA CACGCACCAT CTACACTTCG 
CCCATCAAGG CCCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACATT CGGGGATGTG 
GGGCTGCTCA CCGGGGATGT ACAGCTGCAT CCGGAGGCCT CCTGCCTCAT CATGACCACA 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCAGATGTTA TTCGGGACCT GGAGTGGGTC 
ATCTTTGATG AGGTTCACTA TATCAACGAT GTCGAGCGTG GGGTCGTGTG GGAGGAGGTG 
CTTATCATGC TACCTGACCA CGTTTCTATC ATCCTTCTGA GTGCCACCGT CCCCAACGCC 
CTTGAGTTTG CTGACTGGAT TGGGCGGCTG AAGCGTCGTC AGATCTATGT GATTAGCACT 
GTAACCCGCC CCGTGCCCCT GGAGCACTAT CTTTTCACAG GGAACAGCTC CAAGACCCAG 
GGGGAGCTCT TTTTGTTGCT GGACTCCCGA GGAGCCTTCC ATACAAAAGG GTACTATGCA 
GCTGTGGAGG CCAAGAAGGA GAGAATGAGC AAACACGCCC AGACCTTTGG GGCCAAGCAG 
CCCACACATC AGGGGGGCCC TGCACAGGAC CGCGGAGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACAC GTGCCCAGTT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAT 
GAGCAGGCCT CAGGCCTCAC CTCCCTTGAC CTCACCACCA GTTCGGAGAA GAGCGAGATC 
CACCTCTTCC TGCAGCGCTG CCTTGCTCGC CTCCGTGGCT CTGACCGCCA GCTGCCCCAG 
GTCCTGCACA TGTCAGAGCT CCTGAATCGC GGCCTGGGTG TGCACCATAG CGGCATCCTG 
CCCATCCTCA AGGAGATCGT GGAGATGCTC TTCAGCCGTG GCCTGGTCAA GGTCTTGTTT 
GCCACAGAGA CCTTTGCCAT GGGAGTAAAC ATGCCTGCTC GTACAGTAGT GTTTGACTCC 
ATGCGCAAAC ACGATGGCTC CACCTTCCGG GACCTGCTCC CTGGGGAGTA TGTGCAGATG 
GCAGGCCGGG CAGGGCGGAG GGGCCTGGAC CCCACAGGCA CCGTTATCCT GCTCTGCAAG 
GGCCGAGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC GTCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACTATG ATCCTCAACT TGCTGCGAGT GGATGCCCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCTGAGTTTC CCTCCCGCAA AGACAGCAAG 
GCCCATGAAC AGGCCCTGGC TGAACTGACC AAGAGGCTGG GAGCTTTGGA GGAGCCTGAC 
ATGACTGGCC AACTGGTCGA CCTGCCTGAA TATTACAGCT GGGGGGAGGA ACTGACAGAG 
ACCCAGCACA TGATCCAGCG ACGCATCATG GAGTCTGTGA ACGGGCTGAA GTCTCTCTCA 
GCAGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCATTGGG AGTGATCCTA 
CAGGTCTCCT CGAACTCCAC CAGCAGAGTA TTCACAACCC TGGTCTTGTG TGATAAGCCC 
TTGTCCCAGG ACCCACAGGA CAGGGGGCCA GCCACTGCAG AGGTGCCCTA TCCAGATGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAA GGGCCTTGTG ACCACACCGT GGTCAAGCTC 
CAGCCAGGAG ATATGGCTGC CATCACCACC AAGGTGCTCC GGGTGAATGG GGAGAAGATC 
TTGGAGGACT TCAGCAAGAG GCAGCAGCCA AAATTCAAGA AGGATCCTCC CCTTGCAGCC 
GTGACCACTG CTGTCCAGGA ACTGCTGCGT CTGGCTCAGG CCCACCCAGC CGGACCTCCC 
ACCCTCGACC CTGTCAATGA CCTGCAGCTC AAAGATATGT CAGTTGTAGA GGGTGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCAG GGGGCTCAGT GTGTACACAG CCCCCGTTTT 
CCTGCCCAGT ACCTGAAGCT GCGGGAGCGA ATGCAGATAC AGAAGGAGAT GGAGCGGCTG 
CGCTTCCTAC TGTCGGATCA GTCATTGCTG CTGCTTCCTG AGTACCATCA GCGAGTAGAG 
GTGCTCCGAA CCCTGGGTTA TGTGGACGAG GCGGGCACTG TGAAGCTGGC AGGGCGGGTG 
GCTTGTGCCA TGAGCAGCCA TGAGTTGCTC CTCACTGAGC TCATGTTTGA CAATGCACTG 
AGCACCCTGC GGCCTGAGGA GATTGCTGCC TTGCTCTCTG GCCTGGTCTG CCAGAGCCCT 
GGGGACGCTG GGGATCAGCT CCCAAACACC CTCAAGCAGG GAATAGAACG TGTCCGGGCT 
GTGGCCAAGC GGATTGGTGA GGTCCAGGTG GCTTGTGGCC TGAACCAGAC GGTGGAGGAA 
TTTGTGGGGG AGCTGAATTT TGGGCTGGTT GAGGTTGTAT ATGAGTGGGC CCGGGGCATG 
CCCTTCTCCG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATT 
CAGCGCCTGG CTGAGATGTG TCGCTCACTG CGGGGGGCAG CCCGCCTGGT AGGAGAGCCT 
GTGCTGGGTG CCAAGATGGA GACAGCGGCT ACCTTGCTAC GGCGGGACAT CGTATTTGCG 
GCCAGCCTCT ACACCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIV2L ( NM_006929.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_008860.4 Homo sapiens Ski2 like RNA helicase (SKIV2L), mRNA.

Price & Availability↑

CloneID	OHu25855
Clone ID Related Accession (Same CDS sequence)	NM_006929.4 , NM_006929.5
Accession Version	NM_006929.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	helicase SKI2W
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U09877.1, AL529319.3, BG396326.1, BE395851.1 and Z48796.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006929.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z48796.1, U09877.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000375394.7/ ENSP00000364543.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGGAGA CAGAGCGACT TGTGCTACCC CCTCCAGATC CCCTGGACCT ACCCCTTCGG 
GCCGTGGAGC TCGGATGCAC GGGGCACTGG GAGCTGCTGA ACTTGCCTGG AGCTCCAGAG 
AGTAGCCTTC CCCATGGCCT CCCTCCTTGT GCCCCAGATC TGCAGCAAGA AGCAGAACAG 
TTGTTTCTGT CATCCCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGAAAA 
TGGCAGAGGA AGACGGATCC CTGGTCTCTT TTGGCTGTCC TGGGAGCCCC AGTCCCATCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TGGGTTACAA AGAGGTCTTG 
CTGGAGAACA CAAATCTCTC GGCTACAACC TCCTTGTCTC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CCTTATGGGG AAATCCAACT CAGTATCCCT TCTGGCCAGG GGGGATGGAT 
GAACCCACCA TAACAGATCT GAACACACGG GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TTACTATTCC ACCTGGTTTC AAGAAAGGCA TGGACTTTGC ACCAAAAGAT 
TGTCCAACTC CAGCTCCTGG ACTACTAAGC CTTAGCTGTA TGTTGGAGCC TCTGGATTTG 
GGTGGGGGTG ACGAGGATGA GAATGAGGCA GTGGGACAGC CAGGAGGTCC CAGAGGGGAC 
ACTGTTTCAG CCTCTCCCTG CAGTGCTCCC CTGGCCCGAG CAAGCAGCTT GGAAGACCTA 
GTGTTGAAGG AAGCGTCCAC AGCTGTATCC ACCCCAGAGG CCCCAGAGCC TCCATCTCAG 
GAGCAGTGGG CCATCCCTGT GGACGCCACC TCCCCTGTTG GTGATTTCTA TCGCCTCATT 
CCCCAGCCAG CCTTCCAGTG GGCATTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAAC GGCATGACTC TGTCTTTGTC GCAGCTCACA CATCTGCAGG AAAAACAGTT 
GTGGCTGAAT ATGCCATTGC CCTGGCCCAG AAACACATGA CACGCACCAT CTACACTTCG 
CCCATCAAGG CCCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACATT CGGGGATGTG 
GGGCTGCTCA CCGGGGATGT ACAGCTGCAT CCGGAGGCCT CCTGCCTCAT CATGACCACA 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCAGATGTTA TTCGGGACCT GGAGTGGGTC 
ATCTTTGATG AGGTTCACTA TATCAACGAT GTCGAGCGTG GGGTCGTGTG GGAGGAGGTG 
CTTATCATGC TACCTGACCA CGTTTCTATC ATCCTTCTGA GTGCCACCGT CCCCAACGCC 
CTTGAGTTTG CTGACTGGAT TGGGCGGCTG AAGCGTCGTC AGATCTATGT GATTAGCACT 
GTAACCCGCC CCGTGCCCCT GGAGCACTAT CTTTTCACAG GGAACAGCTC CAAGACCCAG 
GGGGAGCTCT TTTTGTTGCT GGACTCCCGA GGAGCCTTCC ATACAAAAGG GTACTATGCA 
GCTGTGGAGG CCAAGAAGGA GAGAATGAGC AAACACGCCC AGACCTTTGG GGCCAAGCAG 
CCCACACATC AGGGGGGCCC TGCACAGGAC CGCGGAGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACAC GTGCCCAGTT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAT 
GAGCAGGCCT CAGGCCTCAC CTCCCTTGAC CTCACCACCA GTTCGGAGAA GAGCGAGATC 
CACCTCTTCC TGCAGCGCTG CCTTGCTCGC CTCCGTGGCT CTGACCGCCA GCTGCCCCAG 
GTCCTGCACA TGTCAGAGCT CCTGAATCGC GGCCTGGGTG TGCACCATAG CGGCATCCTG 
CCCATCCTCA AGGAGATCGT GGAGATGCTC TTCAGCCGTG GCCTGGTCAA GGTCTTGTTT 
GCCACAGAGA CCTTTGCCAT GGGAGTAAAC ATGCCTGCTC GTACAGTAGT GTTTGACTCC 
ATGCGCAAAC ACGATGGCTC CACCTTCCGG GACCTGCTCC CTGGGGAGTA TGTGCAGATG 
GCAGGCCGGG CAGGGCGGAG GGGCCTGGAC CCCACAGGCA CCGTTATCCT GCTCTGCAAG 
GGCCGAGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC GTCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACTATG ATCCTCAACT TGCTGCGAGT GGATGCCCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCTGAGTTTC CCTCCCGCAA AGACAGCAAG 
GCCCATGAAC AGGCCCTGGC TGAACTGACC AAGAGGCTGG GAGCTTTGGA GGAGCCTGAC 
ATGACTGGCC AACTGGTCGA CCTGCCTGAA TATTACAGCT GGGGGGAGGA ACTGACAGAG 
ACCCAGCACA TGATCCAGCG ACGCATCATG GAGTCTGTGA ACGGGCTGAA GTCTCTCTCA 
GCAGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCATTGGG AGTGATCCTA 
CAGGTCTCCT CGAACTCCAC CAGCAGAGTA TTCACAACCC TGGTCTTGTG TGATAAGCCC 
TTGTCCCAGG ACCCACAGGA CAGGGGGCCA GCCACTGCAG AGGTGCCCTA TCCAGATGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAA GGGCCTTGTG ACCACACCGT GGTCAAGCTC 
CAGCCAGGAG ATATGGCTGC CATCACCACC AAGGTGCTCC GGGTGAATGG GGAGAAGATC 
TTGGAGGACT TCAGCAAGAG GCAGCAGCCA AAATTCAAGA AGGATCCTCC CCTTGCAGCC 
GTGACCACTG CTGTCCAGGA ACTGCTGCGT CTGGCTCAGG CCCACCCAGC CGGACCTCCC 
ACCCTCGACC CTGTCAATGA CCTGCAGCTC AAAGATATGT CAGTTGTAGA GGGTGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCAG GGGGCTCAGT GTGTACACAG CCCCCGTTTT 
CCTGCCCAGT ACCTGAAGCT GCGGGAGCGA ATGCAGATAC AGAAGGAGAT GGAGCGGCTG 
CGCTTCCTAC TGTCGGATCA GTCATTGCTG CTGCTTCCTG AGTACCATCA GCGAGTAGAG 
GTGCTCCGAA CCCTGGGTTA TGTGGACGAG GCGGGCACTG TGAAGCTGGC AGGGCGGGTG 
GCTTGTGCCA TGAGCAGCCA TGAGTTGCTC CTCACTGAGC TCATGTTTGA CAATGCACTG 
AGCACCCTGC GGCCTGAGGA GATTGCTGCC TTGCTCTCTG GCCTGGTCTG CCAGAGCCCT 
GGGGACGCTG GGGATCAGCT CCCAAACACC CTCAAGCAGG GAATAGAACG TGTCCGGGCT 
GTGGCCAAGC GGATTGGTGA GGTCCAGGTG GCTTGTGGCC TGAACCAGAC GGTGGAGGAA 
TTTGTGGGGG AGCTGAATTT TGGGCTGGTT GAGGTTGTAT ATGAGTGGGC CCGGGGCATG 
CCCTTCTCCG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATT 
CAGCGCCTGG CTGAGATGTG TCGCTCACTG CGGGGGGCAG CCCGCCTGGT AGGAGAGCCT 
GTGCTGGGTG CCAAGATGGA GACAGCGGCT ACCTTGCTAC GGCGGGACAT CGTATTTGCG 
GCCAGCCTCT ACACCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008860.4
CDS	19..3759
Translation	MMETERLVLPPPDPLDLPLRAVELGCTGHWELLNLPGAPESSLPHGLPPCAPDLQQEAEQLFLSSPAWLPLHGVEHSARKWQRKTDPWSLLAVLGAPVPSDLQAQRHPTTGQILGYKEVLLENTNLSATTSLSLRRPPGPASQSLWGNPTQYPFWPGGMDEPTITDLNTREEAEEEIDFEKDLLTIPPGFKKGMDFAPKDCPTPAPGLLSLSCMLEPLDLGGGDEDENEAVGQPGGPRGDTVSASPCSAPLARASSLEDLVLKEASTAVSTPEAPEPPSQEQWAIPVDATSPVGDFYRLIPQPAFQWAFEPDVFQKQAILHLERHDSVFVAAHTSAGKTVVAEYAIALAQKHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPEASCLIMTTEILRSMLYSGSDVIRDLEWVIFDEVHYINDVERGVVWEEVLIMLPDHVSIILLSATVPNALEFADWIGRLKRRQIYVISTVTRPVPLEHYLFTGNSSKTQGELFLLLDSRGAFHTKGYYAAVEAKKERMSKHAQTFGAKQPTHQGGPAQDRGVYLSLLASLRTRAQLPVVVFTFSRGRCDEQASGLTSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHMSELLNRGLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMRKHDGSTFRDLLPGEYVQMAGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMMGKPSQLQSQFRLTYTMILNLLRVDALRVEDMMKRSFSEFPSRKDSKAHEQALAELTKRLGALEEPDMTGQLVDLPEYYSWGEELTETQHMIQRRIMESVNGLKSLSAGRVVVVKNQEHHNALGVILQVSSNSTSRVFTTLVLCDKPLSQDPQDRGPATAEVPYPDDLVGFKLFLPEGPCDHTVVKLQPGDMAAITTKVLRVNGEKILEDFSKRQQPKFKKDPPLAAVTTAVQELLRLAQAHPAGPPTLDPVNDLQLKDMSVVEGGLRARKLEELIQGAQCVHSPRFPAQYLKLRERMQIQKEMERLRFLLSDQSLLLLPEYHQRVEVLRTLGYVDEAGTVKLAGRVACAMSSHELLLTELMFDNALSTLRPEEIAALLSGLVCQSPGDAGDQLPNTLKQGIERVRAVAKRIGEVQVACGLNQTVEEFVGELNFGLVEVVYEWARGMPFSELAGLSGTPEGLVVRCIQRLAEMCRSLRGAARLVGEPVLGAKMETAATLLRRDIVFAASLYTQ

Target ORF information:

RefSeq Version	NM_006929.5
Organism	Homo sapiens(human)
Definition	Homo sapiens Ski2 like RNA helicase (SKIV2L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006929.5

ORF Insert Sequence:

ATGATGGAGA CAGAGCGACT TGTGCTACCC CCTCCAGATC CCCTGGACCT ACCCCTTCGG 
GCCGTGGAGC TCGGATGCAC GGGGCACTGG GAGCTGCTGA ACTTGCCTGG AGCTCCAGAG 
AGTAGCCTTC CCCATGGCCT CCCTCCTTGT GCCCCAGATC TGCAGCAAGA AGCAGAACAG 
TTGTTTCTGT CATCCCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGAAAA 
TGGCAGAGGA AGACGGATCC CTGGTCTCTT TTGGCTGTCC TGGGAGCCCC AGTCCCATCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TGGGTTACAA AGAGGTCTTG 
CTGGAGAACA CAAATCTCTC GGCTACAACC TCCTTGTCTC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CCTTATGGGG AAATCCAACT CAGTATCCCT TCTGGCCAGG GGGGATGGAT 
GAACCCACCA TAACAGATCT GAACACACGG GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TTACTATTCC ACCTGGTTTC AAGAAAGGCA TGGACTTTGC ACCAAAAGAT 
TGTCCAACTC CAGCTCCTGG ACTACTAAGC CTTAGCTGTA TGTTGGAGCC TCTGGATTTG 
GGTGGGGGTG ACGAGGATGA GAATGAGGCA GTGGGACAGC CAGGAGGTCC CAGAGGGGAC 
ACTGTTTCAG CCTCTCCCTG CAGTGCTCCC CTGGCCCGAG CAAGCAGCTT GGAAGACCTA 
GTGTTGAAGG AAGCGTCCAC AGCTGTATCC ACCCCAGAGG CCCCAGAGCC TCCATCTCAG 
GAGCAGTGGG CCATCCCTGT GGACGCCACC TCCCCTGTTG GTGATTTCTA TCGCCTCATT 
CCCCAGCCAG CCTTCCAGTG GGCATTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAAC GGCATGACTC TGTCTTTGTC GCAGCTCACA CATCTGCAGG AAAAACAGTT 
GTGGCTGAAT ATGCCATTGC CCTGGCCCAG AAACACATGA CACGCACCAT CTACACTTCG 
CCCATCAAGG CCCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACATT CGGGGATGTG 
GGGCTGCTCA CCGGGGATGT ACAGCTGCAT CCGGAGGCCT CCTGCCTCAT CATGACCACA 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCAGATGTTA TTCGGGACCT GGAGTGGGTC 
ATCTTTGATG AGGTTCACTA TATCAACGAT GTCGAGCGTG GGGTCGTGTG GGAGGAGGTG 
CTTATCATGC TACCTGACCA CGTTTCTATC ATCCTTCTGA GTGCCACCGT CCCCAACGCC 
CTTGAGTTTG CTGACTGGAT TGGGCGGCTG AAGCGTCGTC AGATCTATGT GATTAGCACT 
GTAACCCGCC CCGTGCCCCT GGAGCACTAT CTTTTCACAG GGAACAGCTC CAAGACCCAG 
GGGGAGCTCT TTTTGTTGCT GGACTCCCGA GGAGCCTTCC ATACAAAAGG GTACTATGCA 
GCTGTGGAGG CCAAGAAGGA GAGAATGAGC AAACACGCCC AGACCTTTGG GGCCAAGCAG 
CCCACACATC AGGGGGGCCC TGCACAGGAC CGCGGAGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACAC GTGCCCAGTT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAT 
GAGCAGGCCT CAGGCCTCAC CTCCCTTGAC CTCACCACCA GTTCGGAGAA GAGCGAGATC 
CACCTCTTCC TGCAGCGCTG CCTTGCTCGC CTCCGTGGCT CTGACCGCCA GCTGCCCCAG 
GTCCTGCACA TGTCAGAGCT CCTGAATCGC GGCCTGGGTG TGCACCATAG CGGCATCCTG 
CCCATCCTCA AGGAGATCGT GGAGATGCTC TTCAGCCGTG GCCTGGTCAA GGTCTTGTTT 
GCCACAGAGA CCTTTGCCAT GGGAGTAAAC ATGCCTGCTC GTACAGTAGT GTTTGACTCC 
ATGCGCAAAC ACGATGGCTC CACCTTCCGG GACCTGCTCC CTGGGGAGTA TGTGCAGATG 
GCAGGCCGGG CAGGGCGGAG GGGCCTGGAC CCCACAGGCA CCGTTATCCT GCTCTGCAAG 
GGCCGAGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC GTCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACTATG ATCCTCAACT TGCTGCGAGT GGATGCCCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCTGAGTTTC CCTCCCGCAA AGACAGCAAG 
GCCCATGAAC AGGCCCTGGC TGAACTGACC AAGAGGCTGG GAGCTTTGGA GGAGCCTGAC 
ATGACTGGCC AACTGGTCGA CCTGCCTGAA TATTACAGCT GGGGGGAGGA ACTGACAGAG 
ACCCAGCACA TGATCCAGCG ACGCATCATG GAGTCTGTGA ACGGGCTGAA GTCTCTCTCA 
GCAGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCATTGGG AGTGATCCTA 
CAGGTCTCCT CGAACTCCAC CAGCAGAGTA TTCACAACCC TGGTCTTGTG TGATAAGCCC 
TTGTCCCAGG ACCCACAGGA CAGGGGGCCA GCCACTGCAG AGGTGCCCTA TCCAGATGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAA GGGCCTTGTG ACCACACCGT GGTCAAGCTC 
CAGCCAGGAG ATATGGCTGC CATCACCACC AAGGTGCTCC GGGTGAATGG GGAGAAGATC 
TTGGAGGACT TCAGCAAGAG GCAGCAGCCA AAATTCAAGA AGGATCCTCC CCTTGCAGCC 
GTGACCACTG CTGTCCAGGA ACTGCTGCGT CTGGCTCAGG CCCACCCAGC CGGACCTCCC 
ACCCTCGACC CTGTCAATGA CCTGCAGCTC AAAGATATGT CAGTTGTAGA GGGTGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCAG GGGGCTCAGT GTGTACACAG CCCCCGTTTT 
CCTGCCCAGT ACCTGAAGCT GCGGGAGCGA ATGCAGATAC AGAAGGAGAT GGAGCGGCTG 
CGCTTCCTAC TGTCGGATCA GTCATTGCTG CTGCTTCCTG AGTACCATCA GCGAGTAGAG 
GTGCTCCGAA CCCTGGGTTA TGTGGACGAG GCGGGCACTG TGAAGCTGGC AGGGCGGGTG 
GCTTGTGCCA TGAGCAGCCA TGAGTTGCTC CTCACTGAGC TCATGTTTGA CAATGCACTG 
AGCACCCTGC GGCCTGAGGA GATTGCTGCC TTGCTCTCTG GCCTGGTCTG CCAGAGCCCT 
GGGGACGCTG GGGATCAGCT CCCAAACACC CTCAAGCAGG GAATAGAACG TGTCCGGGCT 
GTGGCCAAGC GGATTGGTGA GGTCCAGGTG GCTTGTGGCC TGAACCAGAC GGTGGAGGAA 
TTTGTGGGGG AGCTGAATTT TGGGCTGGTT GAGGTTGTAT ATGAGTGGGC CCGGGGCATG 
CCCTTCTCCG AGTTGGCAGG GCTCTCAGGG ACCCCTGAGG GCCTGGTGGT CCGCTGCATT 
CAGCGCCTGG CTGAGATGTG TCGCTCACTG CGGGGGGCAG CCCGCCTGGT AGGAGAGCCT 
GTGCTGGGTG CCAAGATGGA GACAGCGGCT ACCTTGCTAC GGCGGGACAT CGTATTTGCG 
GCCAGCCTCT ACACCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIV2L ( XM_011514815.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513117.1 Homo sapiens Ski2 like RNA helicase (SKIV2L), transcript variant X1, mRNA.

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CloneID	OHu60526
Clone ID Related Accession (Same CDS sequence)	XM_011514815.3 , XM_011514815.2
Accession Version	XM_011514815.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	helicase SKI2W isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA CAGAGCGACT TGTGCTACCC CCTCCAGATC CCCTGGACCT ACCCCTTCGG 
GCCGTGGAGC TCGGATGCAC GGGGCACTGG GAGCTGCTGA ACTTGCCTGG AGCTCCAGAG 
AGTAGCCTTC CCCATGGCCT CCCTCCTTGT GCCCCAGATC TGCAGCAAGA AGCAGAACAG 
TTGTTTCTGT CATCCCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGAAAA 
TGGCAGAGGA AGACGGATCC CTGGTCTCTT TTGGCTGTCC TGGGAGCCCC AGTCCCATCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TGGGTTACAA AGAGGTCTTG 
CTGGAGAACA CAAATCTCTC GGCTACAACC TCCTTGTCTC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CCTTATGGGG AAATCCAACT CAGTATCCCT TCTGGCCAGG GGGGATGGAT 
GAACCCACCA TAACAGATCT GAACACACGG GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TTACTATTCC ACCTGGTTTC AAGAAAGGCA TGGACTTTGC ACCAAAAGAT 
TGTCCAACTC CAGCTCCTGG ACTACTAAGC CTTAGCTGTA TGTTGGAGCC TCTGGATTTG 
GGTGGGGGTG ACGAGGATGA GAATGAGGCA GTGGGACAGC CAGGAGGTCC CAGAGGGGAC 
ACTGTTTCAG CCTCTCCCTG CAGTGCTCCC CTGGCCCGAG CAAGCAGCTT GGAAGACCTA 
GTGTTGAAGG AAGCGTCCAC AGCTGTATCC ACCCCAGAGG CCCCAGAGCC TCCATCTCAG 
GAGCAGTGGG CCATCCCTGT GGACGCCACC TCCCCTGTTG GTGATTTCTA TCGCCTCATT 
CCCCAGCCAG CCTTCCAGTG GGCATTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAAC GGCATGACTC TGTCTTTGTC GCAGCTCACA CATCTGCAGG AAAAACAGTT 
GTGGCTGAAT ATGCCATTGC CCTGGCCCAG AAACACATGA CACGCACCAT CTACACTTCG 
CCCATCAAGG CCCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACATT CGGGGATGTG 
GGGCTGCTCA CCGGGGATGT ACAGCTGCAT CCGGAGGCCT CCTGCCTCAT CATGACCACA 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCAGATGTTA TTCGGGACCT GGAGTGGGTC 
ATCTTTGATG AGGTTCACTA TATCAACGAT GTCGAGCGTG GGGTCGTGTG GGAGGAGGTG 
CTTATCATGC TACCTGACCA CGTTTCTATC ATCCTTCTGA GTGCCACCGT CCCCAACGCC 
CTTGAGTTTG CTGACTGGAT TGGGCGGCTG AAGCGTCGTC AGATCTATGT GATTAGCACT 
GTAACCCGCC CCGTGCCCCT GGAGCACTAT CTTTTCACAG GGAACAGCTC CAAGACCCAG 
GGGGAGCTCT TTTTGTTGCT GGACTCCCGA GGAGCCTTCC ATACAAAAGG GTACTATGCA 
GCTGTGGAGG CCAAGAAGGA GAGAATGAGC AAACACGCCC AGACCTTTGG GGCCAAGCAG 
CCCACACATC AGGGGGGCCC TGCACAGGAC CGCGGAGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACAC GTGCCCAGTT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAT 
GAGCAGGCCT CAGGCCTCAC CTCCCTTGAC CTCACCACCA GTTCGGAGAA GAGCGAGATC 
CACCTCTTCC TGCAGCGCTG CCTTGCTCGC CTCCGTGGCT CTGACCGCCA GCTGCCCCAG 
GTCCTGCACA TGTCAGAGCT CCTGAATCGC GGCCTGGGTG TGCACCATAG CGGCATCCTG 
CCCATCCTCA AGGAGATCGT GGAGATGCTC TTCAGCCGTG GCCTGGTCAA GGTCTTGTTT 
GCCACAGAGA CCTTTGCCAT GGGAGTAAAC ATGCCTGCTC GTACAGTAGT GTTTGACTCC 
ATGCGCAAAC ACGATGGCTC CACCTTCCGG GACCTGCTCC CTGGGGAGTA TGTGCAGATG 
GCAGGCCGGG CAGGGCGGAG GGGCCTGGAC CCCACAGGCA CCGTTATCCT GCTCTGCAAG 
GGCCGAGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC GTCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACTATG ATCCTCAACT TGCTGCGAGT GGATGCCCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCTGAGTTTC CCTCCCGCAA AGACAGCAAG 
GCCCATGAAC AGGCCCTGGC TGAACTGACC AAGAGGCTGG GAGCTTTGGA GGAGCCTGAC 
ATGACTGGCC AACTGGTCGA CCTGCCTGAA TATTACAGCT GGGGGGAGGA ACTGACAGAG 
ACCCAGCACA TGATCCAGCG ACGCATCATG GAGTCTGTGA ACGGGCTGAA GTCTCTCTCA 
GCAGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCATTGGG AGTGATCCTA 
CAGGTCTCCT CGAACTCCAC CAGCAGAGTA TTCACAACCC TGGTCTTGTG TGATAAGCCC 
TTGTCCCAGG ACCCACAGGA CAGGGGGCCA GCCACTGCAG AGGTGCCCTA TCCAGATGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAA GGGCCTTGTG ACCACACCGT GGTCAAGCTC 
CAGCCAGGAG ATATGGCTGC CATCACCACC AAGGTGCTCC GGGTGAATGG GGAGAAGATC 
TTGGAGGACT TCAGCAAGAG GCAGCAGCCA AAATTCAAGA AGGATCCTCC CCTTGCAGCC 
GTGACCACTG CTGTCCAGGA ACTGCTGCGT CTGGCTCAGG CCCACCCAGC CGGACCTCCC 
ACCCTCGACC CTGTCAATGA CCTGCAGCTC AAAGATATGT CAGTTGTAGA GGGTGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCAG GGGGCTCAGT GTGTACACAG CCCCCGTTTT 
CCTGCCCAGT GCACCCCTGC TAAGGGGCAA GGAGAAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513117.1
CDS	37..3138
Translation	MMETERLVLPPPDPLDLPLRAVELGCTGHWELLNLPGAPESSLPHGLPPCAPDLQQEAEQLFLSSPAWLPLHGVEHSARKWQRKTDPWSLLAVLGAPVPSDLQAQRHPTTGQILGYKEVLLENTNLSATTSLSLRRPPGPASQSLWGNPTQYPFWPGGMDEPTITDLNTREEAEEEIDFEKDLLTIPPGFKKGMDFAPKDCPTPAPGLLSLSCMLEPLDLGGGDEDENEAVGQPGGPRGDTVSASPCSAPLARASSLEDLVLKEASTAVSTPEAPEPPSQEQWAIPVDATSPVGDFYRLIPQPAFQWAFEPDVFQKQAILHLERHDSVFVAAHTSAGKTVVAEYAIALAQKHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPEASCLIMTTEILRSMLYSGSDVIRDLEWVIFDEVHYINDVERGVVWEEVLIMLPDHVSIILLSATVPNALEFADWIGRLKRRQIYVISTVTRPVPLEHYLFTGNSSKTQGELFLLLDSRGAFHTKGYYAAVEAKKERMSKHAQTFGAKQPTHQGGPAQDRGVYLSLLASLRTRAQLPVVVFTFSRGRCDEQASGLTSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHMSELLNRGLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMRKHDGSTFRDLLPGEYVQMAGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMMGKPSQLQSQFRLTYTMILNLLRVDALRVEDMMKRSFSEFPSRKDSKAHEQALAELTKRLGALEEPDMTGQLVDLPEYYSWGEELTETQHMIQRRIMESVNGLKSLSAGRVVVVKNQEHHNALGVILQVSSNSTSRVFTTLVLCDKPLSQDPQDRGPATAEVPYPDDLVGFKLFLPEGPCDHTVVKLQPGDMAAITTKVLRVNGEKILEDFSKRQQPKFKKDPPLAAVTTAVQELLRLAQAHPAGPPTLDPVNDLQLKDMSVVEGGLRARKLEELIQGAQCVHSPRFPAQCTPAKGQGEG

Target ORF information:

RefSeq Version	XM_011514815.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Ski2 like RNA helicase (SKIV2L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514815.3

ORF Insert Sequence:

ATGATGGAGA CAGAGCGACT TGTGCTACCC CCTCCAGATC CCCTGGACCT ACCCCTTCGG 
GCCGTGGAGC TCGGATGCAC GGGGCACTGG GAGCTGCTGA ACTTGCCTGG AGCTCCAGAG 
AGTAGCCTTC CCCATGGCCT CCCTCCTTGT GCCCCAGATC TGCAGCAAGA AGCAGAACAG 
TTGTTTCTGT CATCCCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGAAAA 
TGGCAGAGGA AGACGGATCC CTGGTCTCTT TTGGCTGTCC TGGGAGCCCC AGTCCCATCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TGGGTTACAA AGAGGTCTTG 
CTGGAGAACA CAAATCTCTC GGCTACAACC TCCTTGTCTC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CCTTATGGGG AAATCCAACT CAGTATCCCT TCTGGCCAGG GGGGATGGAT 
GAACCCACCA TAACAGATCT GAACACACGG GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TTACTATTCC ACCTGGTTTC AAGAAAGGCA TGGACTTTGC ACCAAAAGAT 
TGTCCAACTC CAGCTCCTGG ACTACTAAGC CTTAGCTGTA TGTTGGAGCC TCTGGATTTG 
GGTGGGGGTG ACGAGGATGA GAATGAGGCA GTGGGACAGC CAGGAGGTCC CAGAGGGGAC 
ACTGTTTCAG CCTCTCCCTG CAGTGCTCCC CTGGCCCGAG CAAGCAGCTT GGAAGACCTA 
GTGTTGAAGG AAGCGTCCAC AGCTGTATCC ACCCCAGAGG CCCCAGAGCC TCCATCTCAG 
GAGCAGTGGG CCATCCCTGT GGACGCCACC TCCCCTGTTG GTGATTTCTA TCGCCTCATT 
CCCCAGCCAG CCTTCCAGTG GGCATTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAAC GGCATGACTC TGTCTTTGTC GCAGCTCACA CATCTGCAGG AAAAACAGTT 
GTGGCTGAAT ATGCCATTGC CCTGGCCCAG AAACACATGA CACGCACCAT CTACACTTCG 
CCCATCAAGG CCCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACATT CGGGGATGTG 
GGGCTGCTCA CCGGGGATGT ACAGCTGCAT CCGGAGGCCT CCTGCCTCAT CATGACCACA 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCAGATGTTA TTCGGGACCT GGAGTGGGTC 
ATCTTTGATG AGGTTCACTA TATCAACGAT GTCGAGCGTG GGGTCGTGTG GGAGGAGGTG 
CTTATCATGC TACCTGACCA CGTTTCTATC ATCCTTCTGA GTGCCACCGT CCCCAACGCC 
CTTGAGTTTG CTGACTGGAT TGGGCGGCTG AAGCGTCGTC AGATCTATGT GATTAGCACT 
GTAACCCGCC CCGTGCCCCT GGAGCACTAT CTTTTCACAG GGAACAGCTC CAAGACCCAG 
GGGGAGCTCT TTTTGTTGCT GGACTCCCGA GGAGCCTTCC ATACAAAAGG GTACTATGCA 
GCTGTGGAGG CCAAGAAGGA GAGAATGAGC AAACACGCCC AGACCTTTGG GGCCAAGCAG 
CCCACACATC AGGGGGGCCC TGCACAGGAC CGCGGAGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACAC GTGCCCAGTT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAT 
GAGCAGGCCT CAGGCCTCAC CTCCCTTGAC CTCACCACCA GTTCGGAGAA GAGCGAGATC 
CACCTCTTCC TGCAGCGCTG CCTTGCTCGC CTCCGTGGCT CTGACCGCCA GCTGCCCCAG 
GTCCTGCACA TGTCAGAGCT CCTGAATCGC GGCCTGGGTG TGCACCATAG CGGCATCCTG 
CCCATCCTCA AGGAGATCGT GGAGATGCTC TTCAGCCGTG GCCTGGTCAA GGTCTTGTTT 
GCCACAGAGA CCTTTGCCAT GGGAGTAAAC ATGCCTGCTC GTACAGTAGT GTTTGACTCC 
ATGCGCAAAC ACGATGGCTC CACCTTCCGG GACCTGCTCC CTGGGGAGTA TGTGCAGATG 
GCAGGCCGGG CAGGGCGGAG GGGCCTGGAC CCCACAGGCA CCGTTATCCT GCTCTGCAAG 
GGCCGAGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC GTCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACTATG ATCCTCAACT TGCTGCGAGT GGATGCCCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCTGAGTTTC CCTCCCGCAA AGACAGCAAG 
GCCCATGAAC AGGCCCTGGC TGAACTGACC AAGAGGCTGG GAGCTTTGGA GGAGCCTGAC 
ATGACTGGCC AACTGGTCGA CCTGCCTGAA TATTACAGCT GGGGGGAGGA ACTGACAGAG 
ACCCAGCACA TGATCCAGCG ACGCATCATG GAGTCTGTGA ACGGGCTGAA GTCTCTCTCA 
GCAGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCATTGGG AGTGATCCTA 
CAGGTCTCCT CGAACTCCAC CAGCAGAGTA TTCACAACCC TGGTCTTGTG TGATAAGCCC 
TTGTCCCAGG ACCCACAGGA CAGGGGGCCA GCCACTGCAG AGGTGCCCTA TCCAGATGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAA GGGCCTTGTG ACCACACCGT GGTCAAGCTC 
CAGCCAGGAG ATATGGCTGC CATCACCACC AAGGTGCTCC GGGTGAATGG GGAGAAGATC 
TTGGAGGACT TCAGCAAGAG GCAGCAGCCA AAATTCAAGA AGGATCCTCC CCTTGCAGCC 
GTGACCACTG CTGTCCAGGA ACTGCTGCGT CTGGCTCAGG CCCACCCAGC CGGACCTCCC 
ACCCTCGACC CTGTCAATGA CCTGCAGCTC AAAGATATGT CAGTTGTAGA GGGTGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCAG GGGGCTCAGT GTGTACACAG CCCCCGTTTT 
CCTGCCCAGT GCACCCCTGC TAAGGGGCAA GGAGAAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SKIV2L ( XM_011514815.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513117.1 Homo sapiens Ski2 like RNA helicase (SKIV2L), transcript variant X1, mRNA.

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CloneID	OHu60526
Clone ID Related Accession (Same CDS sequence)	XM_011514815.3 , XM_011514815.2
Accession Version	XM_011514815.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	helicase SKI2W isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514815.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA CAGAGCGACT TGTGCTACCC CCTCCAGATC CCCTGGACCT ACCCCTTCGG 
GCCGTGGAGC TCGGATGCAC GGGGCACTGG GAGCTGCTGA ACTTGCCTGG AGCTCCAGAG 
AGTAGCCTTC CCCATGGCCT CCCTCCTTGT GCCCCAGATC TGCAGCAAGA AGCAGAACAG 
TTGTTTCTGT CATCCCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGAAAA 
TGGCAGAGGA AGACGGATCC CTGGTCTCTT TTGGCTGTCC TGGGAGCCCC AGTCCCATCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TGGGTTACAA AGAGGTCTTG 
CTGGAGAACA CAAATCTCTC GGCTACAACC TCCTTGTCTC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CCTTATGGGG AAATCCAACT CAGTATCCCT TCTGGCCAGG GGGGATGGAT 
GAACCCACCA TAACAGATCT GAACACACGG GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TTACTATTCC ACCTGGTTTC AAGAAAGGCA TGGACTTTGC ACCAAAAGAT 
TGTCCAACTC CAGCTCCTGG ACTACTAAGC CTTAGCTGTA TGTTGGAGCC TCTGGATTTG 
GGTGGGGGTG ACGAGGATGA GAATGAGGCA GTGGGACAGC CAGGAGGTCC CAGAGGGGAC 
ACTGTTTCAG CCTCTCCCTG CAGTGCTCCC CTGGCCCGAG CAAGCAGCTT GGAAGACCTA 
GTGTTGAAGG AAGCGTCCAC AGCTGTATCC ACCCCAGAGG CCCCAGAGCC TCCATCTCAG 
GAGCAGTGGG CCATCCCTGT GGACGCCACC TCCCCTGTTG GTGATTTCTA TCGCCTCATT 
CCCCAGCCAG CCTTCCAGTG GGCATTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAAC GGCATGACTC TGTCTTTGTC GCAGCTCACA CATCTGCAGG AAAAACAGTT 
GTGGCTGAAT ATGCCATTGC CCTGGCCCAG AAACACATGA CACGCACCAT CTACACTTCG 
CCCATCAAGG CCCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACATT CGGGGATGTG 
GGGCTGCTCA CCGGGGATGT ACAGCTGCAT CCGGAGGCCT CCTGCCTCAT CATGACCACA 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCAGATGTTA TTCGGGACCT GGAGTGGGTC 
ATCTTTGATG AGGTTCACTA TATCAACGAT GTCGAGCGTG GGGTCGTGTG GGAGGAGGTG 
CTTATCATGC TACCTGACCA CGTTTCTATC ATCCTTCTGA GTGCCACCGT CCCCAACGCC 
CTTGAGTTTG CTGACTGGAT TGGGCGGCTG AAGCGTCGTC AGATCTATGT GATTAGCACT 
GTAACCCGCC CCGTGCCCCT GGAGCACTAT CTTTTCACAG GGAACAGCTC CAAGACCCAG 
GGGGAGCTCT TTTTGTTGCT GGACTCCCGA GGAGCCTTCC ATACAAAAGG GTACTATGCA 
GCTGTGGAGG CCAAGAAGGA GAGAATGAGC AAACACGCCC AGACCTTTGG GGCCAAGCAG 
CCCACACATC AGGGGGGCCC TGCACAGGAC CGCGGAGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACAC GTGCCCAGTT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAT 
GAGCAGGCCT CAGGCCTCAC CTCCCTTGAC CTCACCACCA GTTCGGAGAA GAGCGAGATC 
CACCTCTTCC TGCAGCGCTG CCTTGCTCGC CTCCGTGGCT CTGACCGCCA GCTGCCCCAG 
GTCCTGCACA TGTCAGAGCT CCTGAATCGC GGCCTGGGTG TGCACCATAG CGGCATCCTG 
CCCATCCTCA AGGAGATCGT GGAGATGCTC TTCAGCCGTG GCCTGGTCAA GGTCTTGTTT 
GCCACAGAGA CCTTTGCCAT GGGAGTAAAC ATGCCTGCTC GTACAGTAGT GTTTGACTCC 
ATGCGCAAAC ACGATGGCTC CACCTTCCGG GACCTGCTCC CTGGGGAGTA TGTGCAGATG 
GCAGGCCGGG CAGGGCGGAG GGGCCTGGAC CCCACAGGCA CCGTTATCCT GCTCTGCAAG 
GGCCGAGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC GTCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACTATG ATCCTCAACT TGCTGCGAGT GGATGCCCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCTGAGTTTC CCTCCCGCAA AGACAGCAAG 
GCCCATGAAC AGGCCCTGGC TGAACTGACC AAGAGGCTGG GAGCTTTGGA GGAGCCTGAC 
ATGACTGGCC AACTGGTCGA CCTGCCTGAA TATTACAGCT GGGGGGAGGA ACTGACAGAG 
ACCCAGCACA TGATCCAGCG ACGCATCATG GAGTCTGTGA ACGGGCTGAA GTCTCTCTCA 
GCAGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCATTGGG AGTGATCCTA 
CAGGTCTCCT CGAACTCCAC CAGCAGAGTA TTCACAACCC TGGTCTTGTG TGATAAGCCC 
TTGTCCCAGG ACCCACAGGA CAGGGGGCCA GCCACTGCAG AGGTGCCCTA TCCAGATGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAA GGGCCTTGTG ACCACACCGT GGTCAAGCTC 
CAGCCAGGAG ATATGGCTGC CATCACCACC AAGGTGCTCC GGGTGAATGG GGAGAAGATC 
TTGGAGGACT TCAGCAAGAG GCAGCAGCCA AAATTCAAGA AGGATCCTCC CCTTGCAGCC 
GTGACCACTG CTGTCCAGGA ACTGCTGCGT CTGGCTCAGG CCCACCCAGC CGGACCTCCC 
ACCCTCGACC CTGTCAATGA CCTGCAGCTC AAAGATATGT CAGTTGTAGA GGGTGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCAG GGGGCTCAGT GTGTACACAG CCCCCGTTTT 
CCTGCCCAGT GCACCCCTGC TAAGGGGCAA GGAGAAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513117.1
CDS	97..3198
Translation	MMETERLVLPPPDPLDLPLRAVELGCTGHWELLNLPGAPESSLPHGLPPCAPDLQQEAEQLFLSSPAWLPLHGVEHSARKWQRKTDPWSLLAVLGAPVPSDLQAQRHPTTGQILGYKEVLLENTNLSATTSLSLRRPPGPASQSLWGNPTQYPFWPGGMDEPTITDLNTREEAEEEIDFEKDLLTIPPGFKKGMDFAPKDCPTPAPGLLSLSCMLEPLDLGGGDEDENEAVGQPGGPRGDTVSASPCSAPLARASSLEDLVLKEASTAVSTPEAPEPPSQEQWAIPVDATSPVGDFYRLIPQPAFQWAFEPDVFQKQAILHLERHDSVFVAAHTSAGKTVVAEYAIALAQKHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPEASCLIMTTEILRSMLYSGSDVIRDLEWVIFDEVHYINDVERGVVWEEVLIMLPDHVSIILLSATVPNALEFADWIGRLKRRQIYVISTVTRPVPLEHYLFTGNSSKTQGELFLLLDSRGAFHTKGYYAAVEAKKERMSKHAQTFGAKQPTHQGGPAQDRGVYLSLLASLRTRAQLPVVVFTFSRGRCDEQASGLTSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHMSELLNRGLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMRKHDGSTFRDLLPGEYVQMAGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMMGKPSQLQSQFRLTYTMILNLLRVDALRVEDMMKRSFSEFPSRKDSKAHEQALAELTKRLGALEEPDMTGQLVDLPEYYSWGEELTETQHMIQRRIMESVNGLKSLSAGRVVVVKNQEHHNALGVILQVSSNSTSRVFTTLVLCDKPLSQDPQDRGPATAEVPYPDDLVGFKLFLPEGPCDHTVVKLQPGDMAAITTKVLRVNGEKILEDFSKRQQPKFKKDPPLAAVTTAVQELLRLAQAHPAGPPTLDPVNDLQLKDMSVVEGGLRARKLEELIQGAQCVHSPRFPAQCTPAKGQGEG

Target ORF information:

RefSeq Version	XM_011514815.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Ski2 like RNA helicase (SKIV2L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514815.2

ORF Insert Sequence:

ATGATGGAGA CAGAGCGACT TGTGCTACCC CCTCCAGATC CCCTGGACCT ACCCCTTCGG 
GCCGTGGAGC TCGGATGCAC GGGGCACTGG GAGCTGCTGA ACTTGCCTGG AGCTCCAGAG 
AGTAGCCTTC CCCATGGCCT CCCTCCTTGT GCCCCAGATC TGCAGCAAGA AGCAGAACAG 
TTGTTTCTGT CATCCCCAGC CTGGCTGCCT CTGCATGGTG TGGAGCACTC AGCCCGAAAA 
TGGCAGAGGA AGACGGATCC CTGGTCTCTT TTGGCTGTCC TGGGAGCCCC AGTCCCATCC 
GACCTACAGG CCCAAAGACA CCCAACCACA GGCCAGATAC TGGGTTACAA AGAGGTCTTG 
CTGGAGAACA CAAATCTCTC GGCTACAACC TCCTTGTCTC TTCGCCGGCC TCCAGGGCCA 
GCCTCCCAGT CCTTATGGGG AAATCCAACT CAGTATCCCT TCTGGCCAGG GGGGATGGAT 
GAACCCACCA TAACAGATCT GAACACACGG GAGGAGGCTG AGGAGGAGAT AGACTTTGAG 
AAAGATCTTC TTACTATTCC ACCTGGTTTC AAGAAAGGCA TGGACTTTGC ACCAAAAGAT 
TGTCCAACTC CAGCTCCTGG ACTACTAAGC CTTAGCTGTA TGTTGGAGCC TCTGGATTTG 
GGTGGGGGTG ACGAGGATGA GAATGAGGCA GTGGGACAGC CAGGAGGTCC CAGAGGGGAC 
ACTGTTTCAG CCTCTCCCTG CAGTGCTCCC CTGGCCCGAG CAAGCAGCTT GGAAGACCTA 
GTGTTGAAGG AAGCGTCCAC AGCTGTATCC ACCCCAGAGG CCCCAGAGCC TCCATCTCAG 
GAGCAGTGGG CCATCCCTGT GGACGCCACC TCCCCTGTTG GTGATTTCTA TCGCCTCATT 
CCCCAGCCAG CCTTCCAGTG GGCATTTGAG CCAGATGTGT TTCAGAAACA GGCCATCCTG 
CACTTGGAAC GGCATGACTC TGTCTTTGTC GCAGCTCACA CATCTGCAGG AAAAACAGTT 
GTGGCTGAAT ATGCCATTGC CCTGGCCCAG AAACACATGA CACGCACCAT CTACACTTCG 
CCCATCAAGG CCCTGAGCAA CCAGAAGTTC CGGGACTTCC GAAACACATT CGGGGATGTG 
GGGCTGCTCA CCGGGGATGT ACAGCTGCAT CCGGAGGCCT CCTGCCTCAT CATGACCACA 
GAGATCCTTC GCTCCATGCT GTACAGTGGC TCAGATGTTA TTCGGGACCT GGAGTGGGTC 
ATCTTTGATG AGGTTCACTA TATCAACGAT GTCGAGCGTG GGGTCGTGTG GGAGGAGGTG 
CTTATCATGC TACCTGACCA CGTTTCTATC ATCCTTCTGA GTGCCACCGT CCCCAACGCC 
CTTGAGTTTG CTGACTGGAT TGGGCGGCTG AAGCGTCGTC AGATCTATGT GATTAGCACT 
GTAACCCGCC CCGTGCCCCT GGAGCACTAT CTTTTCACAG GGAACAGCTC CAAGACCCAG 
GGGGAGCTCT TTTTGTTGCT GGACTCCCGA GGAGCCTTCC ATACAAAAGG GTACTATGCA 
GCTGTGGAGG CCAAGAAGGA GAGAATGAGC AAACACGCCC AGACCTTTGG GGCCAAGCAG 
CCCACACATC AGGGGGGCCC TGCACAGGAC CGCGGAGTGT ACCTGTCCCT CCTGGCCTCC 
CTCCGCACAC GTGCCCAGTT GCCCGTGGTG GTGTTCACCT TCTCCCGGGG CCGCTGTGAT 
GAGCAGGCCT CAGGCCTCAC CTCCCTTGAC CTCACCACCA GTTCGGAGAA GAGCGAGATC 
CACCTCTTCC TGCAGCGCTG CCTTGCTCGC CTCCGTGGCT CTGACCGCCA GCTGCCCCAG 
GTCCTGCACA TGTCAGAGCT CCTGAATCGC GGCCTGGGTG TGCACCATAG CGGCATCCTG 
CCCATCCTCA AGGAGATCGT GGAGATGCTC TTCAGCCGTG GCCTGGTCAA GGTCTTGTTT 
GCCACAGAGA CCTTTGCCAT GGGAGTAAAC ATGCCTGCTC GTACAGTAGT GTTTGACTCC 
ATGCGCAAAC ACGATGGCTC CACCTTCCGG GACCTGCTCC CTGGGGAGTA TGTGCAGATG 
GCAGGCCGGG CAGGGCGGAG GGGCCTGGAC CCCACAGGCA CCGTTATCCT GCTCTGCAAG 
GGCCGAGTGC CCGAGATGGC AGACCTGCAC CGCATGATGA TGGGGAAGCC GTCCCAGCTG 
CAGTCCCAGT TCCGCCTCAC GTACACTATG ATCCTCAACT TGCTGCGAGT GGATGCCCTC 
AGGGTGGAGG ACATGATGAA GAGGAGCTTC TCTGAGTTTC CCTCCCGCAA AGACAGCAAG 
GCCCATGAAC AGGCCCTGGC TGAACTGACC AAGAGGCTGG GAGCTTTGGA GGAGCCTGAC 
ATGACTGGCC AACTGGTCGA CCTGCCTGAA TATTACAGCT GGGGGGAGGA ACTGACAGAG 
ACCCAGCACA TGATCCAGCG ACGCATCATG GAGTCTGTGA ACGGGCTGAA GTCTCTCTCA 
GCAGGAAGGG TGGTGGTTGT GAAGAATCAG GAGCATCACA ACGCATTGGG AGTGATCCTA 
CAGGTCTCCT CGAACTCCAC CAGCAGAGTA TTCACAACCC TGGTCTTGTG TGATAAGCCC 
TTGTCCCAGG ACCCACAGGA CAGGGGGCCA GCCACTGCAG AGGTGCCCTA TCCAGATGAC 
CTCGTGGGAT TCAAGCTGTT CCTGCCTGAA GGGCCTTGTG ACCACACCGT GGTCAAGCTC 
CAGCCAGGAG ATATGGCTGC CATCACCACC AAGGTGCTCC GGGTGAATGG GGAGAAGATC 
TTGGAGGACT TCAGCAAGAG GCAGCAGCCA AAATTCAAGA AGGATCCTCC CCTTGCAGCC 
GTGACCACTG CTGTCCAGGA ACTGCTGCGT CTGGCTCAGG CCCACCCAGC CGGACCTCCC 
ACCCTCGACC CTGTCAATGA CCTGCAGCTC AAAGATATGT CAGTTGTAGA GGGTGGGCTC 
CGGGCCCGGA AGCTGGAGGA GCTGATCCAG GGGGCTCAGT GTGTACACAG CCCCCGTTTT 
CCTGCCCAGT GCACCCCTGC TAAGGGGCAA GGAGAAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association between SKIV2L polymorphism rs429608 and age-related macular degeneration: A meta-analysis.
Ophthalmic genetics38(3)245-251(2017 May-Jun)
Shuai P,Ye Z,Liu Y,Qu C,Liu X,Luo H,Feng X,Li X,Shi Y,Gong B

Targeted next-generation sequencing identification of a novel missense mutation of the SKIV2L gene in a patient with trichohepatoenteric syndrome.
Molecular medicine reports14(3)2107-10(2016 Sep)
Zheng B,Pan J,Jin Y,Wang C,Liu Z

Novel mutations in SKIV2L and TTC37 genes in Malaysian children with trichohepatoenteric syndrome.
Gene586(1)1-6(2016 Jul)
Lee WS,Teo KM,Ng RT,Chong SY,Kee BP,Chua KH

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SKIV2L cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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