tst cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tst
Entrez Gene ID	42813
Full Name	twister
Synonyms	CG10210,Dmel\CG10210,SKI2,Ski2,Ski2/tst,cg10210,dSki2
General protein information	Preferred Names twister Names CG10210 gene product from transcript CG10210-RA CG10210-PA CG10210-PB tst-PA tst-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95B1-95B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275955.1	NP_001262884.1	twister, isoform B
NM_079741.3	NP_524465.1	twister, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003182	XP_312880.4
Homo sapiens (human)	SKIV2L	NP_008860.4
Danio rerio (zebrafish)	skiv2l	NP_001038366.1
Schizosaccharomyces pombe (fission yeast)	SPCC550.03c	NP_588095.1
Canis lupus familiaris (dog)	SKIV2L	XP_538841.2
Bos taurus (cattle)	SKIV2L	XP_003587743.1
Arabidopsis thaliana (thale cress)	AT3G46960	NP_190280.5
Pan troglodytes (chimpanzee)	SKIV2L	XP_001160927.2
	SKIV2L	XP_001106475.2
Rattus norvegicus (Norway rat)	Skiv2l	NP_998724.1
Drosophila melanogaster (fruit fly)	tst	NP_524465.1
Caenorhabditis elegans (roundworm)	skih-2	NP_502084.2
Saccharomyces cerevisiae (baker's yeast)	SKI2	NP_013502.3
Xenopus tropicalis (tropical clawed frog)	skiv2l	XP_004917184.1
Mus musculus (house mouse)	Skiv2l	NP_067312.2
Eremothecium gossypii	AGOS_AER067C	NP_984927.2
Neurospora crassa	NCU01857	XP_965488.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tst gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tst cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05837	NM_001275955.1 Latest version!	Drosophila melanogaster twister (tst), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa05837	NM_079741.3 Latest version!	Drosophila melanogaster twister (tst), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tst ( NM_001275955.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262884.1 Drosophila melanogaster twister (tst), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05837
Clone ID Related Accession (Same CDS sequence)	NM_001275955.1 , NM_079741.3
Accession Version	NM_001275955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twister, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGAGA TTAGGGGCGA GGATAACCTC GAACGGCTGC GCAACTATAT CCTGCGACCG 
GAGCTGTCCC TCCACAATCC CCTGCCGGAT GTTTTGCCCC GCAAATTCGA TCCGATGCGC 
CTGTTGCATG CGCCCCGTTG CCCGGGAAGC ACTAAGTTGG CTCCTCGCCG CGACCAGAGT 
GGTCACATAC TTGAGTTCGT GGAATTGGAC TTGGAGGCCG TGGGCGCCAA TGCCAACAAC 
TCGATGTCCA TGCAGCGGGA GCCGGGCTTG CTGGAAGACG CGACGCGTGG CTCCCACTCC 
AATTTTCCCT TTTGGCCAGG CGGTTTCGAT GAGCAGCGCC AGCAGATCGC AGCTCTGGAT 
GTCAGCAACT TTCAGTTCGG GGACAAGCTG CTAACGGTGC CACCGGGCTT CAGCTCTGGC 
TACGACTTCT TCCAATCGCA ATCTGTAACA CTGCCGCCTG TGGTCGCCGA TCCCAGTAAC 
GTTGATCTAT TGGAAAACCT AGAACAGGAT CTGGACGTCC AGGAGTGGAT GAAGCTTACG 
CGTAGTGAGG AAACAAGCTC CACTGGAACG CCTAAAAGTC CAAAACAGTC GAACGTGCAA 
TTACCTTCAG AGGAGTTCAG AGACGTGGAC GATCACATTA TGAAGGCTGA CCTAAAGCCT 
GTGCTAAACA TTTCCACCAC AACCAAGAAC TTCAAGAGCG ATTGGGCCGA AATGGTGGAC 
ATAAGCCAAC CCATCAATAA CTTTAAAGAG CAGATACCAT GCCCCGCCAT GGATTTCCCC 
TTCGAACTGG ATGTCTTTCA AAAGCAGGCC ATTCTCAAGC TGGAACAACG CCAGTACGTT 
TTCGTTGCAG CGCATACATC TGCTGGCAAG ACAGTTGTAG CTGAATATGC CATCGCTCTG 
TCCAAGCGTG ATCTCACCCG CACCATCTAC ACATCGCCGA TCAAGGCGTT GTCCAATCAG 
AAGTACCGCG ACTTCCGAAA AACCTTCAAG GATGTGGGCC TTATTACAGG TGACCTGCAG 
ATAGAGCCCA CCGCCTCTTG TCTGATCATG ACCACCGAGA TTCTCCGCTC AATGCTGTAT 
TGCGGCAGCG ATGTCACGCG GGATCTGGAG TGGGTGATCT TCGACGAGGT GCACTATATT 
AATAACCCAG AAAGAGGACA TGTCTGGGAG GAGGTCATTA TACTCCTGCC CGAACATGTT 
AACATTATAA TGCTCAGTGC CACCGTGCCC AATACCTTGG AATTGGCCGA CTGGGTTGGG 
AGCACCAAGA AGAGAAAGGT CTATGTGATC AGCACTTTAA AACGACCAGT GCCTCTGACG 
CATTTCCTCT ATACTGGTGC TGGAGGAAAG AGTCGCGATG ACATCTTCCT CCTGGTGGAC 
GCCCAGGGAA AATATCTGCA GGGTAACTAC GAGAAGGCCG TGGAGCGCAA GAAGGAGATG 
CAAGGCAAGG CCAAGGGCGG CGGTGGTGGG CCAAGAAACC ATTTAAATGC CAAGCAGGAG 
CAGTACACCT GGATCGGACT AATTGACTTC CTGAGGCGGA GCAACATGAT GCCAGTGGTG 
GCTTTCACTC TGTCCCGCAA TCGGTGCGAC TCCAATTTGG CCGCTTTGCA GTCAGTGGAC 
CTGAACACGG AAAAGGAGAA GGGTGCAGTG CAGAAGTTCT TCCTGCAGTG TCTGGCAAAG 
CTGAAGCCAC CGGACCGTAC AATTCCGCAG GTTCTTGTTC TCAAGGACGC TTTGGAGAGA 
GGAATAGGTG TGCATCATAG CGGCATCTTG CCCATTCTTA AGGAGATTGT CGAGATGCTC 
TTTCAAAATG GGCTAGTTAA GCTGCTTTTC GCCACCGAAA CGTTTGCCAT GGGCGTCAAC 
ATGCCCGCGC GCACTGTGGT CTTCGACTCC TGCAAAAAGT TTGACGGACT GGAGATGCGT 
AACCTGAAAC CAGGCGAATA CATCCAAATG GCCGGCAGAG CTGGACGCCG TGGGCACGAC 
GAGACAGGTA CGGCGATCAT TATGTGCAAG GGCAGTGTGC CACCATCGAT GGAGCTACGG 
CCCATGATTC TGGGCCTGCC GGAGAAGTTG CAGTCACAGT TCATTCTGCG CTATGCGGTA 
ATCCTCACAT GTCTGCGCAT CGAGAGTATC AAGGTGGAGG ATATCATGAA GTTCAGTTTC 
AAGGAGTTTA ACCTAAAGCT CCAATTGCCT ACGCAGCAGA AGCAACTTCG TCTGGCCGAA 
GACAAGTTTG CCATGCTCCC AACTCTCGGC GAACATCTGC AACCACTTGT CAATTTTTAT 
GACAAAGCCG TGGAGTATTG GAAAGAAAAG CACCGCATCA TGAAATTTGT TGTTACTCAA 
CCCAAGATCC AAAAGGAACT GAAAGCTGGC CGCGTCATTG TGATCACGCA GGGCAAGCAC 
TACAACAAAC TGGCCATTCT ACTCAACACC AAATCTGTCC CGGGCAAGGA TACCATCTAT 
AAGGTGCTAG TGCTGGACCA TCAATTCAAG GCAAAGGATA GCAACAGCCT CCAGCAAGGC 
GAGCTCTATT ACAAAATACT TTCGCTGACG CCTAGAAACA TGACCTTCCA ACCAGTGGGA 
ATCGGAGGAC ACACTGTGCT GGACATCAAG GCTATAGACA TTATCAGTAT CACCAAGAGC 
ACATTGAAAG TCGATGCAGA TGCGATTATC CGGAATTGGG AGCAGCGGCA GCTGGAGCGG 
TTCAAGGACT CCCCGCCAAG TGGATCGGTG GTCAAGGCGG TGACCGAGCT AAACCAGCTC 
AATGAGAGTT ACATTGACAA CCCGGATAAC ATCAAATACG TTAATCTTTC CAAGGAAATA 
ATCGTGAACG CTGACAGCGA GGTGGCCATG CTGAACTACG TTGATCACCT GCTCCGTAAG 
GTTGGCGAAT TTCTGCCACA TACCAACATT GCCGGATTCG AACAGGAGTT CGCCAAAGTG 
TACGAGCGCC GAATGCTGGA GATTCACATC GAGGAGCTGC GCTTCAAGAA CTCCGCGAGA 
AATTTGACCC TCTATCCGGA CTATTGTAAC AAATTGAAGG TGCTGCGAGC CCTGAAATAC 
ATAGATGAAC TGGACGAGGT CACACTTAAG GGAAAAGTGG CCTGTGAGAT GGGCCAAAAT 
GAGCTACTTA TCACGGAGCT CATACTATGC AACATGTTCA ACGATCTCGA GCCGGCAGAA 
ATAGCCGCCT TGCTCTCCGG CCTTGTCTTC CAGGCGAAGA TGCAGGATAA GCCGGTAATA 
CCAGAGGCGT TGAAGGAGTG CGTGGCGGCA TTTGAGCAGA TCAACGATAC AATTCTTGCC 
GAGGAGCAGC GCTTTCAGGC AGCCATCGAG ACCGATAATC GCCTGAACTT TGGTCTGCTG 
GAGGTGGTCT ACGAGTGGGC TAGAAATAAG CCCTTTGCTG AGATTATGAA ACTGACGACG 
GTGCAGGAGG GCATCATTGT TCGCTGCATT CAGCAGCTGA ATGAAACGCT GCGTGATGTT 
AAAACGGCCG CCATTCGGAT TGGCAATCCG GGACTGCAGT CGAAGATGGA GGAGGCGTCG 
GCTGCTATCA AGCGCGATAT TGTTTTCACC GCATCCTTGT ACACAGAACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262884.1
CDS	98..3691
Translation	MNEIRGEDNLERLRNYILRPELSLHNPLPDVLPRKFDPMRLLHAPRCPGSTKLAPRRDQSGHILEFVELDLEAVGANANNSMSMQREPGLLEDATRGSHSNFPFWPGGFDEQRQQIAALDVSNFQFGDKLLTVPPGFSSGYDFFQSQSVTLPPVVADPSNVDLLENLEQDLDVQEWMKLTRSEETSSTGTPKSPKQSNVQLPSEEFRDVDDHIMKADLKPVLNISTTTKNFKSDWAEMVDISQPINNFKEQIPCPAMDFPFELDVFQKQAILKLEQRQYVFVAAHTSAGKTVVAEYAIALSKRDLTRTIYTSPIKALSNQKYRDFRKTFKDVGLITGDLQIEPTASCLIMTTEILRSMLYCGSDVTRDLEWVIFDEVHYINNPERGHVWEEVIILLPEHVNIIMLSATVPNTLELADWVGSTKKRKVYVISTLKRPVPLTHFLYTGAGGKSRDDIFLLVDAQGKYLQGNYEKAVERKKEMQGKAKGGGGGPRNHLNAKQEQYTWIGLIDFLRRSNMMPVVAFTLSRNRCDSNLAALQSVDLNTEKEKGAVQKFFLQCLAKLKPPDRTIPQVLVLKDALERGIGVHHSGILPILKEIVEMLFQNGLVKLLFATETFAMGVNMPARTVVFDSCKKFDGLEMRNLKPGEYIQMAGRAGRRGHDETGTAIIMCKGSVPPSMELRPMILGLPEKLQSQFILRYAVILTCLRIESIKVEDIMKFSFKEFNLKLQLPTQQKQLRLAEDKFAMLPTLGEHLQPLVNFYDKAVEYWKEKHRIMKFVVTQPKIQKELKAGRVIVITQGKHYNKLAILLNTKSVPGKDTIYKVLVLDHQFKAKDSNSLQQGELYYKILSLTPRNMTFQPVGIGGHTVLDIKAIDIISITKSTLKVDADAIIRNWEQRQLERFKDSPPSGSVVKAVTELNQLNESYIDNPDNIKYVNLSKEIIVNADSEVAMLNYVDHLLRKVGEFLPHTNIAGFEQEFAKVYERRMLEIHIEELRFKNSARNLTLYPDYCNKLKVLRALKYIDELDEVTLKGKVACEMGQNELLITELILCNMFNDLEPAEIAALLSGLVFQAKMQDKPVIPEALKECVAAFEQINDTILAEEQRFQAAIETDNRLNFGLLEVVYEWARNKPFAEIMKLTTVQEGIIVRCIQQLNETLRDVKTAAIRIGNPGLQSKMEEASAAIKRDIVFTASLYTEL

Target ORF information:

RefSeq Version	NM_001275955.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twister (tst), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275955.1

ORF Insert Sequence:

ATGAATGAGA TTAGGGGCGA GGATAACCTC GAACGGCTGC GCAACTATAT CCTGCGACCG 
GAGCTGTCCC TCCACAATCC CCTGCCGGAT GTTTTGCCCC GCAAATTCGA TCCGATGCGC 
CTGTTGCATG CGCCCCGTTG CCCGGGAAGC ACTAAGTTGG CTCCTCGCCG CGACCAGAGT 
GGTCACATAC TTGAGTTCGT GGAATTGGAC TTGGAGGCCG TGGGCGCCAA TGCCAACAAC 
TCGATGTCCA TGCAGCGGGA GCCGGGCTTG CTGGAAGACG CGACGCGTGG CTCCCACTCC 
AATTTTCCCT TTTGGCCAGG CGGTTTCGAT GAGCAGCGCC AGCAGATCGC AGCTCTGGAT 
GTCAGCAACT TTCAGTTCGG GGACAAGCTG CTAACGGTGC CACCGGGCTT CAGCTCTGGC 
TACGACTTCT TCCAATCGCA ATCTGTAACA CTGCCGCCTG TGGTCGCCGA TCCCAGTAAC 
GTTGATCTAT TGGAAAACCT AGAACAGGAT CTGGACGTCC AGGAGTGGAT GAAGCTTACG 
CGTAGTGAGG AAACAAGCTC CACTGGAACG CCTAAAAGTC CAAAACAGTC GAACGTGCAA 
TTACCTTCAG AGGAGTTCAG AGACGTGGAC GATCACATTA TGAAGGCTGA CCTAAAGCCT 
GTGCTAAACA TTTCCACCAC AACCAAGAAC TTCAAGAGCG ATTGGGCCGA AATGGTGGAC 
ATAAGCCAAC CCATCAATAA CTTTAAAGAG CAGATACCAT GCCCCGCCAT GGATTTCCCC 
TTCGAACTGG ATGTCTTTCA AAAGCAGGCC ATTCTCAAGC TGGAACAACG CCAGTACGTT 
TTCGTTGCAG CGCATACATC TGCTGGCAAG ACAGTTGTAG CTGAATATGC CATCGCTCTG 
TCCAAGCGTG ATCTCACCCG CACCATCTAC ACATCGCCGA TCAAGGCGTT GTCCAATCAG 
AAGTACCGCG ACTTCCGAAA AACCTTCAAG GATGTGGGCC TTATTACAGG TGACCTGCAG 
ATAGAGCCCA CCGCCTCTTG TCTGATCATG ACCACCGAGA TTCTCCGCTC AATGCTGTAT 
TGCGGCAGCG ATGTCACGCG GGATCTGGAG TGGGTGATCT TCGACGAGGT GCACTATATT 
AATAACCCAG AAAGAGGACA TGTCTGGGAG GAGGTCATTA TACTCCTGCC CGAACATGTT 
AACATTATAA TGCTCAGTGC CACCGTGCCC AATACCTTGG AATTGGCCGA CTGGGTTGGG 
AGCACCAAGA AGAGAAAGGT CTATGTGATC AGCACTTTAA AACGACCAGT GCCTCTGACG 
CATTTCCTCT ATACTGGTGC TGGAGGAAAG AGTCGCGATG ACATCTTCCT CCTGGTGGAC 
GCCCAGGGAA AATATCTGCA GGGTAACTAC GAGAAGGCCG TGGAGCGCAA GAAGGAGATG 
CAAGGCAAGG CCAAGGGCGG CGGTGGTGGG CCAAGAAACC ATTTAAATGC CAAGCAGGAG 
CAGTACACCT GGATCGGACT AATTGACTTC CTGAGGCGGA GCAACATGAT GCCAGTGGTG 
GCTTTCACTC TGTCCCGCAA TCGGTGCGAC TCCAATTTGG CCGCTTTGCA GTCAGTGGAC 
CTGAACACGG AAAAGGAGAA GGGTGCAGTG CAGAAGTTCT TCCTGCAGTG TCTGGCAAAG 
CTGAAGCCAC CGGACCGTAC AATTCCGCAG GTTCTTGTTC TCAAGGACGC TTTGGAGAGA 
GGAATAGGTG TGCATCATAG CGGCATCTTG CCCATTCTTA AGGAGATTGT CGAGATGCTC 
TTTCAAAATG GGCTAGTTAA GCTGCTTTTC GCCACCGAAA CGTTTGCCAT GGGCGTCAAC 
ATGCCCGCGC GCACTGTGGT CTTCGACTCC TGCAAAAAGT TTGACGGACT GGAGATGCGT 
AACCTGAAAC CAGGCGAATA CATCCAAATG GCCGGCAGAG CTGGACGCCG TGGGCACGAC 
GAGACAGGTA CGGCGATCAT TATGTGCAAG GGCAGTGTGC CACCATCGAT GGAGCTACGG 
CCCATGATTC TGGGCCTGCC GGAGAAGTTG CAGTCACAGT TCATTCTGCG CTATGCGGTA 
ATCCTCACAT GTCTGCGCAT CGAGAGTATC AAGGTGGAGG ATATCATGAA GTTCAGTTTC 
AAGGAGTTTA ACCTAAAGCT CCAATTGCCT ACGCAGCAGA AGCAACTTCG TCTGGCCGAA 
GACAAGTTTG CCATGCTCCC AACTCTCGGC GAACATCTGC AACCACTTGT CAATTTTTAT 
GACAAAGCCG TGGAGTATTG GAAAGAAAAG CACCGCATCA TGAAATTTGT TGTTACTCAA 
CCCAAGATCC AAAAGGAACT GAAAGCTGGC CGCGTCATTG TGATCACGCA GGGCAAGCAC 
TACAACAAAC TGGCCATTCT ACTCAACACC AAATCTGTCC CGGGCAAGGA TACCATCTAT 
AAGGTGCTAG TGCTGGACCA TCAATTCAAG GCAAAGGATA GCAACAGCCT CCAGCAAGGC 
GAGCTCTATT ACAAAATACT TTCGCTGACG CCTAGAAACA TGACCTTCCA ACCAGTGGGA 
ATCGGAGGAC ACACTGTGCT GGACATCAAG GCTATAGACA TTATCAGTAT CACCAAGAGC 
ACATTGAAAG TCGATGCAGA TGCGATTATC CGGAATTGGG AGCAGCGGCA GCTGGAGCGG 
TTCAAGGACT CCCCGCCAAG TGGATCGGTG GTCAAGGCGG TGACCGAGCT AAACCAGCTC 
AATGAGAGTT ACATTGACAA CCCGGATAAC ATCAAATACG TTAATCTTTC CAAGGAAATA 
ATCGTGAACG CTGACAGCGA GGTGGCCATG CTGAACTACG TTGATCACCT GCTCCGTAAG 
GTTGGCGAAT TTCTGCCACA TACCAACATT GCCGGATTCG AACAGGAGTT CGCCAAAGTG 
TACGAGCGCC GAATGCTGGA GATTCACATC GAGGAGCTGC GCTTCAAGAA CTCCGCGAGA 
AATTTGACCC TCTATCCGGA CTATTGTAAC AAATTGAAGG TGCTGCGAGC CCTGAAATAC 
ATAGATGAAC TGGACGAGGT CACACTTAAG GGAAAAGTGG CCTGTGAGAT GGGCCAAAAT 
GAGCTACTTA TCACGGAGCT CATACTATGC AACATGTTCA ACGATCTCGA GCCGGCAGAA 
ATAGCCGCCT TGCTCTCCGG CCTTGTCTTC CAGGCGAAGA TGCAGGATAA GCCGGTAATA 
CCAGAGGCGT TGAAGGAGTG CGTGGCGGCA TTTGAGCAGA TCAACGATAC AATTCTTGCC 
GAGGAGCAGC GCTTTCAGGC AGCCATCGAG ACCGATAATC GCCTGAACTT TGGTCTGCTG 
GAGGTGGTCT ACGAGTGGGC TAGAAATAAG CCCTTTGCTG AGATTATGAA ACTGACGACG 
GTGCAGGAGG GCATCATTGT TCGCTGCATT CAGCAGCTGA ATGAAACGCT GCGTGATGTT 
AAAACGGCCG CCATTCGGAT TGGCAATCCG GGACTGCAGT CGAAGATGGA GGAGGCGTCG 
GCTGCTATCA AGCGCGATAT TGTTTTCACC GCATCCTTGT ACACAGAACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tst ( NM_079741.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524465.1 Drosophila melanogaster twister (tst), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05837
Clone ID Related Accession (Same CDS sequence)	NM_001275955.1 , NM_079741.3
Accession Version	NM_079741.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	twister, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079741.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGAGA TTAGGGGCGA GGATAACCTC GAACGGCTGC GCAACTATAT CCTGCGACCG 
GAGCTGTCCC TCCACAATCC CCTGCCGGAT GTTTTGCCCC GCAAATTCGA TCCGATGCGC 
CTGTTGCATG CGCCCCGTTG CCCGGGAAGC ACTAAGTTGG CTCCTCGCCG CGACCAGAGT 
GGTCACATAC TTGAGTTCGT GGAATTGGAC TTGGAGGCCG TGGGCGCCAA TGCCAACAAC 
TCGATGTCCA TGCAGCGGGA GCCGGGCTTG CTGGAAGACG CGACGCGTGG CTCCCACTCC 
AATTTTCCCT TTTGGCCAGG CGGTTTCGAT GAGCAGCGCC AGCAGATCGC AGCTCTGGAT 
GTCAGCAACT TTCAGTTCGG GGACAAGCTG CTAACGGTGC CACCGGGCTT CAGCTCTGGC 
TACGACTTCT TCCAATCGCA ATCTGTAACA CTGCCGCCTG TGGTCGCCGA TCCCAGTAAC 
GTTGATCTAT TGGAAAACCT AGAACAGGAT CTGGACGTCC AGGAGTGGAT GAAGCTTACG 
CGTAGTGAGG AAACAAGCTC CACTGGAACG CCTAAAAGTC CAAAACAGTC GAACGTGCAA 
TTACCTTCAG AGGAGTTCAG AGACGTGGAC GATCACATTA TGAAGGCTGA CCTAAAGCCT 
GTGCTAAACA TTTCCACCAC AACCAAGAAC TTCAAGAGCG ATTGGGCCGA AATGGTGGAC 
ATAAGCCAAC CCATCAATAA CTTTAAAGAG CAGATACCAT GCCCCGCCAT GGATTTCCCC 
TTCGAACTGG ATGTCTTTCA AAAGCAGGCC ATTCTCAAGC TGGAACAACG CCAGTACGTT 
TTCGTTGCAG CGCATACATC TGCTGGCAAG ACAGTTGTAG CTGAATATGC CATCGCTCTG 
TCCAAGCGTG ATCTCACCCG CACCATCTAC ACATCGCCGA TCAAGGCGTT GTCCAATCAG 
AAGTACCGCG ACTTCCGAAA AACCTTCAAG GATGTGGGCC TTATTACAGG TGACCTGCAG 
ATAGAGCCCA CCGCCTCTTG TCTGATCATG ACCACCGAGA TTCTCCGCTC AATGCTGTAT 
TGCGGCAGCG ATGTCACGCG GGATCTGGAG TGGGTGATCT TCGACGAGGT GCACTATATT 
AATAACCCAG AAAGAGGACA TGTCTGGGAG GAGGTCATTA TACTCCTGCC CGAACATGTT 
AACATTATAA TGCTCAGTGC CACCGTGCCC AATACCTTGG AATTGGCCGA CTGGGTTGGG 
AGCACCAAGA AGAGAAAGGT CTATGTGATC AGCACTTTAA AACGACCAGT GCCTCTGACG 
CATTTCCTCT ATACTGGTGC TGGAGGAAAG AGTCGCGATG ACATCTTCCT CCTGGTGGAC 
GCCCAGGGAA AATATCTGCA GGGTAACTAC GAGAAGGCCG TGGAGCGCAA GAAGGAGATG 
CAAGGCAAGG CCAAGGGCGG CGGTGGTGGG CCAAGAAACC ATTTAAATGC CAAGCAGGAG 
CAGTACACCT GGATCGGACT AATTGACTTC CTGAGGCGGA GCAACATGAT GCCAGTGGTG 
GCTTTCACTC TGTCCCGCAA TCGGTGCGAC TCCAATTTGG CCGCTTTGCA GTCAGTGGAC 
CTGAACACGG AAAAGGAGAA GGGTGCAGTG CAGAAGTTCT TCCTGCAGTG TCTGGCAAAG 
CTGAAGCCAC CGGACCGTAC AATTCCGCAG GTTCTTGTTC TCAAGGACGC TTTGGAGAGA 
GGAATAGGTG TGCATCATAG CGGCATCTTG CCCATTCTTA AGGAGATTGT CGAGATGCTC 
TTTCAAAATG GGCTAGTTAA GCTGCTTTTC GCCACCGAAA CGTTTGCCAT GGGCGTCAAC 
ATGCCCGCGC GCACTGTGGT CTTCGACTCC TGCAAAAAGT TTGACGGACT GGAGATGCGT 
AACCTGAAAC CAGGCGAATA CATCCAAATG GCCGGCAGAG CTGGACGCCG TGGGCACGAC 
GAGACAGGTA CGGCGATCAT TATGTGCAAG GGCAGTGTGC CACCATCGAT GGAGCTACGG 
CCCATGATTC TGGGCCTGCC GGAGAAGTTG CAGTCACAGT TCATTCTGCG CTATGCGGTA 
ATCCTCACAT GTCTGCGCAT CGAGAGTATC AAGGTGGAGG ATATCATGAA GTTCAGTTTC 
AAGGAGTTTA ACCTAAAGCT CCAATTGCCT ACGCAGCAGA AGCAACTTCG TCTGGCCGAA 
GACAAGTTTG CCATGCTCCC AACTCTCGGC GAACATCTGC AACCACTTGT CAATTTTTAT 
GACAAAGCCG TGGAGTATTG GAAAGAAAAG CACCGCATCA TGAAATTTGT TGTTACTCAA 
CCCAAGATCC AAAAGGAACT GAAAGCTGGC CGCGTCATTG TGATCACGCA GGGCAAGCAC 
TACAACAAAC TGGCCATTCT ACTCAACACC AAATCTGTCC CGGGCAAGGA TACCATCTAT 
AAGGTGCTAG TGCTGGACCA TCAATTCAAG GCAAAGGATA GCAACAGCCT CCAGCAAGGC 
GAGCTCTATT ACAAAATACT TTCGCTGACG CCTAGAAACA TGACCTTCCA ACCAGTGGGA 
ATCGGAGGAC ACACTGTGCT GGACATCAAG GCTATAGACA TTATCAGTAT CACCAAGAGC 
ACATTGAAAG TCGATGCAGA TGCGATTATC CGGAATTGGG AGCAGCGGCA GCTGGAGCGG 
TTCAAGGACT CCCCGCCAAG TGGATCGGTG GTCAAGGCGG TGACCGAGCT AAACCAGCTC 
AATGAGAGTT ACATTGACAA CCCGGATAAC ATCAAATACG TTAATCTTTC CAAGGAAATA 
ATCGTGAACG CTGACAGCGA GGTGGCCATG CTGAACTACG TTGATCACCT GCTCCGTAAG 
GTTGGCGAAT TTCTGCCACA TACCAACATT GCCGGATTCG AACAGGAGTT CGCCAAAGTG 
TACGAGCGCC GAATGCTGGA GATTCACATC GAGGAGCTGC GCTTCAAGAA CTCCGCGAGA 
AATTTGACCC TCTATCCGGA CTATTGTAAC AAATTGAAGG TGCTGCGAGC CCTGAAATAC 
ATAGATGAAC TGGACGAGGT CACACTTAAG GGAAAAGTGG CCTGTGAGAT GGGCCAAAAT 
GAGCTACTTA TCACGGAGCT CATACTATGC AACATGTTCA ACGATCTCGA GCCGGCAGAA 
ATAGCCGCCT TGCTCTCCGG CCTTGTCTTC CAGGCGAAGA TGCAGGATAA GCCGGTAATA 
CCAGAGGCGT TGAAGGAGTG CGTGGCGGCA TTTGAGCAGA TCAACGATAC AATTCTTGCC 
GAGGAGCAGC GCTTTCAGGC AGCCATCGAG ACCGATAATC GCCTGAACTT TGGTCTGCTG 
GAGGTGGTCT ACGAGTGGGC TAGAAATAAG CCCTTTGCTG AGATTATGAA ACTGACGACG 
GTGCAGGAGG GCATCATTGT TCGCTGCATT CAGCAGCTGA ATGAAACGCT GCGTGATGTT 
AAAACGGCCG CCATTCGGAT TGGCAATCCG GGACTGCAGT CGAAGATGGA GGAGGCGTCG 
GCTGCTATCA AGCGCGATAT TGTTTTCACC GCATCCTTGT ACACAGAACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524465.1
CDS	98..3691
Translation	MNEIRGEDNLERLRNYILRPELSLHNPLPDVLPRKFDPMRLLHAPRCPGSTKLAPRRDQSGHILEFVELDLEAVGANANNSMSMQREPGLLEDATRGSHSNFPFWPGGFDEQRQQIAALDVSNFQFGDKLLTVPPGFSSGYDFFQSQSVTLPPVVADPSNVDLLENLEQDLDVQEWMKLTRSEETSSTGTPKSPKQSNVQLPSEEFRDVDDHIMKADLKPVLNISTTTKNFKSDWAEMVDISQPINNFKEQIPCPAMDFPFELDVFQKQAILKLEQRQYVFVAAHTSAGKTVVAEYAIALSKRDLTRTIYTSPIKALSNQKYRDFRKTFKDVGLITGDLQIEPTASCLIMTTEILRSMLYCGSDVTRDLEWVIFDEVHYINNPERGHVWEEVIILLPEHVNIIMLSATVPNTLELADWVGSTKKRKVYVISTLKRPVPLTHFLYTGAGGKSRDDIFLLVDAQGKYLQGNYEKAVERKKEMQGKAKGGGGGPRNHLNAKQEQYTWIGLIDFLRRSNMMPVVAFTLSRNRCDSNLAALQSVDLNTEKEKGAVQKFFLQCLAKLKPPDRTIPQVLVLKDALERGIGVHHSGILPILKEIVEMLFQNGLVKLLFATETFAMGVNMPARTVVFDSCKKFDGLEMRNLKPGEYIQMAGRAGRRGHDETGTAIIMCKGSVPPSMELRPMILGLPEKLQSQFILRYAVILTCLRIESIKVEDIMKFSFKEFNLKLQLPTQQKQLRLAEDKFAMLPTLGEHLQPLVNFYDKAVEYWKEKHRIMKFVVTQPKIQKELKAGRVIVITQGKHYNKLAILLNTKSVPGKDTIYKVLVLDHQFKAKDSNSLQQGELYYKILSLTPRNMTFQPVGIGGHTVLDIKAIDIISITKSTLKVDADAIIRNWEQRQLERFKDSPPSGSVVKAVTELNQLNESYIDNPDNIKYVNLSKEIIVNADSEVAMLNYVDHLLRKVGEFLPHTNIAGFEQEFAKVYERRMLEIHIEELRFKNSARNLTLYPDYCNKLKVLRALKYIDELDEVTLKGKVACEMGQNELLITELILCNMFNDLEPAEIAALLSGLVFQAKMQDKPVIPEALKECVAAFEQINDTILAEEQRFQAAIETDNRLNFGLLEVVYEWARNKPFAEIMKLTTVQEGIIVRCIQQLNETLRDVKTAAIRIGNPGLQSKMEEASAAIKRDIVFTASLYTEL

Target ORF information:

RefSeq Version	NM_079741.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster twister (tst), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079741.3

ORF Insert Sequence:

ATGAATGAGA TTAGGGGCGA GGATAACCTC GAACGGCTGC GCAACTATAT CCTGCGACCG 
GAGCTGTCCC TCCACAATCC CCTGCCGGAT GTTTTGCCCC GCAAATTCGA TCCGATGCGC 
CTGTTGCATG CGCCCCGTTG CCCGGGAAGC ACTAAGTTGG CTCCTCGCCG CGACCAGAGT 
GGTCACATAC TTGAGTTCGT GGAATTGGAC TTGGAGGCCG TGGGCGCCAA TGCCAACAAC 
TCGATGTCCA TGCAGCGGGA GCCGGGCTTG CTGGAAGACG CGACGCGTGG CTCCCACTCC 
AATTTTCCCT TTTGGCCAGG CGGTTTCGAT GAGCAGCGCC AGCAGATCGC AGCTCTGGAT 
GTCAGCAACT TTCAGTTCGG GGACAAGCTG CTAACGGTGC CACCGGGCTT CAGCTCTGGC 
TACGACTTCT TCCAATCGCA ATCTGTAACA CTGCCGCCTG TGGTCGCCGA TCCCAGTAAC 
GTTGATCTAT TGGAAAACCT AGAACAGGAT CTGGACGTCC AGGAGTGGAT GAAGCTTACG 
CGTAGTGAGG AAACAAGCTC CACTGGAACG CCTAAAAGTC CAAAACAGTC GAACGTGCAA 
TTACCTTCAG AGGAGTTCAG AGACGTGGAC GATCACATTA TGAAGGCTGA CCTAAAGCCT 
GTGCTAAACA TTTCCACCAC AACCAAGAAC TTCAAGAGCG ATTGGGCCGA AATGGTGGAC 
ATAAGCCAAC CCATCAATAA CTTTAAAGAG CAGATACCAT GCCCCGCCAT GGATTTCCCC 
TTCGAACTGG ATGTCTTTCA AAAGCAGGCC ATTCTCAAGC TGGAACAACG CCAGTACGTT 
TTCGTTGCAG CGCATACATC TGCTGGCAAG ACAGTTGTAG CTGAATATGC CATCGCTCTG 
TCCAAGCGTG ATCTCACCCG CACCATCTAC ACATCGCCGA TCAAGGCGTT GTCCAATCAG 
AAGTACCGCG ACTTCCGAAA AACCTTCAAG GATGTGGGCC TTATTACAGG TGACCTGCAG 
ATAGAGCCCA CCGCCTCTTG TCTGATCATG ACCACCGAGA TTCTCCGCTC AATGCTGTAT 
TGCGGCAGCG ATGTCACGCG GGATCTGGAG TGGGTGATCT TCGACGAGGT GCACTATATT 
AATAACCCAG AAAGAGGACA TGTCTGGGAG GAGGTCATTA TACTCCTGCC CGAACATGTT 
AACATTATAA TGCTCAGTGC CACCGTGCCC AATACCTTGG AATTGGCCGA CTGGGTTGGG 
AGCACCAAGA AGAGAAAGGT CTATGTGATC AGCACTTTAA AACGACCAGT GCCTCTGACG 
CATTTCCTCT ATACTGGTGC TGGAGGAAAG AGTCGCGATG ACATCTTCCT CCTGGTGGAC 
GCCCAGGGAA AATATCTGCA GGGTAACTAC GAGAAGGCCG TGGAGCGCAA GAAGGAGATG 
CAAGGCAAGG CCAAGGGCGG CGGTGGTGGG CCAAGAAACC ATTTAAATGC CAAGCAGGAG 
CAGTACACCT GGATCGGACT AATTGACTTC CTGAGGCGGA GCAACATGAT GCCAGTGGTG 
GCTTTCACTC TGTCCCGCAA TCGGTGCGAC TCCAATTTGG CCGCTTTGCA GTCAGTGGAC 
CTGAACACGG AAAAGGAGAA GGGTGCAGTG CAGAAGTTCT TCCTGCAGTG TCTGGCAAAG 
CTGAAGCCAC CGGACCGTAC AATTCCGCAG GTTCTTGTTC TCAAGGACGC TTTGGAGAGA 
GGAATAGGTG TGCATCATAG CGGCATCTTG CCCATTCTTA AGGAGATTGT CGAGATGCTC 
TTTCAAAATG GGCTAGTTAA GCTGCTTTTC GCCACCGAAA CGTTTGCCAT GGGCGTCAAC 
ATGCCCGCGC GCACTGTGGT CTTCGACTCC TGCAAAAAGT TTGACGGACT GGAGATGCGT 
AACCTGAAAC CAGGCGAATA CATCCAAATG GCCGGCAGAG CTGGACGCCG TGGGCACGAC 
GAGACAGGTA CGGCGATCAT TATGTGCAAG GGCAGTGTGC CACCATCGAT GGAGCTACGG 
CCCATGATTC TGGGCCTGCC GGAGAAGTTG CAGTCACAGT TCATTCTGCG CTATGCGGTA 
ATCCTCACAT GTCTGCGCAT CGAGAGTATC AAGGTGGAGG ATATCATGAA GTTCAGTTTC 
AAGGAGTTTA ACCTAAAGCT CCAATTGCCT ACGCAGCAGA AGCAACTTCG TCTGGCCGAA 
GACAAGTTTG CCATGCTCCC AACTCTCGGC GAACATCTGC AACCACTTGT CAATTTTTAT 
GACAAAGCCG TGGAGTATTG GAAAGAAAAG CACCGCATCA TGAAATTTGT TGTTACTCAA 
CCCAAGATCC AAAAGGAACT GAAAGCTGGC CGCGTCATTG TGATCACGCA GGGCAAGCAC 
TACAACAAAC TGGCCATTCT ACTCAACACC AAATCTGTCC CGGGCAAGGA TACCATCTAT 
AAGGTGCTAG TGCTGGACCA TCAATTCAAG GCAAAGGATA GCAACAGCCT CCAGCAAGGC 
GAGCTCTATT ACAAAATACT TTCGCTGACG CCTAGAAACA TGACCTTCCA ACCAGTGGGA 
ATCGGAGGAC ACACTGTGCT GGACATCAAG GCTATAGACA TTATCAGTAT CACCAAGAGC 
ACATTGAAAG TCGATGCAGA TGCGATTATC CGGAATTGGG AGCAGCGGCA GCTGGAGCGG 
TTCAAGGACT CCCCGCCAAG TGGATCGGTG GTCAAGGCGG TGACCGAGCT AAACCAGCTC 
AATGAGAGTT ACATTGACAA CCCGGATAAC ATCAAATACG TTAATCTTTC CAAGGAAATA 
ATCGTGAACG CTGACAGCGA GGTGGCCATG CTGAACTACG TTGATCACCT GCTCCGTAAG 
GTTGGCGAAT TTCTGCCACA TACCAACATT GCCGGATTCG AACAGGAGTT CGCCAAAGTG 
TACGAGCGCC GAATGCTGGA GATTCACATC GAGGAGCTGC GCTTCAAGAA CTCCGCGAGA 
AATTTGACCC TCTATCCGGA CTATTGTAAC AAATTGAAGG TGCTGCGAGC CCTGAAATAC 
ATAGATGAAC TGGACGAGGT CACACTTAAG GGAAAAGTGG CCTGTGAGAT GGGCCAAAAT 
GAGCTACTTA TCACGGAGCT CATACTATGC AACATGTTCA ACGATCTCGA GCCGGCAGAA 
ATAGCCGCCT TGCTCTCCGG CCTTGTCTTC CAGGCGAAGA TGCAGGATAA GCCGGTAATA 
CCAGAGGCGT TGAAGGAGTG CGTGGCGGCA TTTGAGCAGA TCAACGATAC AATTCTTGCC 
GAGGAGCAGC GCTTTCAGGC AGCCATCGAG ACCGATAATC GCCTGAACTT TGGTCTGCTG 
GAGGTGGTCT ACGAGTGGGC TAGAAATAAG CCCTTTGCTG AGATTATGAA ACTGACGACG 
GTGCAGGAGG GCATCATTGT TCGCTGCATT CAGCAGCTGA ATGAAACGCT GCGTGATGTT 
AAAACGGCCG CCATTCGGAT TGGCAATCCG GGACTGCAGT CGAAGATGGA GGAGGCGTCG 
GCTGCTATCA AGCGCGATAT TGTTTTCACC GCATCCTTGT ACACAGAACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tst cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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