SPCC550.03c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPCC550.03c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPCC550.03c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99214 NM_001023086.2
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Schizosaccharomyces pombe putative Ski complex RNA helicase Ski2 (SPCC550.03c), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc99214
    Clone ID Related Accession (Same CDS sequence) NM_001023086.2
    Accession Version NM_001023086.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative Ski complex RNA helicase Ski2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023086.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTTCTA AACTTGTAGA TGCAATCAAC GAAGTAGCAG TTTCTAACAA GGATAAAATT 
    GAATTAGATG GAATAAAAGA TGATTCCTTT GACGGACACC TGAGTATCAC TGAAGCAACT
    TACGATGTTG ATACATTTTT GAAGCCTTCT CCAGCCTTAT CGAAGGACTG GATTCGAAAG
    TTGCAAAAAA AATGGGATAG GGAAATTACA TATAAAGGAC TATATGAGTA TCCCGAGACT
    TTAGCTCGTA CGCAAATTCG CTTTCAAAGA CATGGCCTGG AGGGTAAAAT TATGGGCTAC
    AAAGAGGTTC CTGAATTAAT AGAAGACCTT AATTCTAAAA ATTCGTCAAG TTTCTTACGA
    AAACCATCTT CGAAAAATGA ATTCGTTCGT GGAAGCACAA GCAACATTCC TTTTCTGGCA
    GATGACTCTG ATGTCGATGC TATAGCAGGC GAACCATCCG TGAAAATGGC TCTATACGGT
    GAAGATGGTT TGTTGCAAGT ACCGCCAGGG TTCTCCCGAG GGCTTTCCAT GACAGCGACA
    TCCACGACTG ACAATTTGAA CGATGAATTT GACCCCGAAA AATGGGATAC TAAGAAAGTG
    AAAAGCTCGA ATCGTAACTT TGTCACCATT CATGAGTTGA ATGAACACCT AAAAAATGTC
    AATTCTAAAC ATTCTGAAAT TGATGATCTA CTACCTGACA AAAGAAGCAT CGTTTCTCTA
    CCGCCTTCTA CTTTGAACTT GCATAAGCAA CCAGACTATG CTCATGTTGT AGATAGTAGT
    GCACCAATAG AAAATTTTCA ACAACTCGTT CCGGAAATGG CTCTTGACTT TCCTTTTGAA
    CTCGATAATT TTCAAAAGGA GGCTATTTAT CATCTTGAAA TGGGTGACTC TGTCTTTGTT
    GCTGCTCACA CCAGTGCTGG AAAAACGGTA GTAGCTGAAT ATGCAATTGC TTTGGCACAA
    AAACATATGA CGAAGGCTAT ATATACTTCA CCCATTAAAG CACTATCTAA TCAAAAGTTT
    AGAGATTTCA AACATAAATT TGAAGACGTT GGAATTTTAA CTGGTGATGT CCAAGTAAAT
    CCAGAAGGCT CATGCCTTCT TATGACAACA GAAATTTTAC GAAGCATGTT GTATAGAGGT
    GCTGACTTAA TTCGTGATGT TGAGTTTGTT ATCTTTGACG AAGTTCATTA TGTGAATGAT
    TTAGAAAGAG GTGTAGTTTG GGAGGAAGTT ATTATTATGC TTCCTCCTCA TGTAACTTTG
    ATTCTTTTAT CTGCTACCGT CCCTAACACC AAAGAGTTTG CTTCTTGGGT TGGACGAACA
    AAGAAAAAGA ACATTTATGT TATCTCAACT TTGAAGCGAC CAGTGCCCTT AGAGCATTAT
    CTCTGGGTGA AACAGAACAT GTTTAAAATC GTTGATCAGC ACGGTCGGTT TTTGATGGAT
    GGCTACAAAT CCGCAAACGA TGCACTGAAA AAGCCTGATA AACCTGTTAT AGCTAAGGAT
    AATAAGAACT CAGCTAGGGG TCGGGGTGCC GCTAGAGGCA GAGGCGTTCA AACGAATATG
    ATGCGTGGCA GAGGAAGCGC AAAGTCTGTT GAAAGAAGAG ATGCTAACAC TTGGGTTCAC
    TTAATTGGTC ATTTACATAA GCAAAATCTT TTGCCTGTAA TAGTATTTGT ATTTTCGAAG
    AAACGCTGTG AGGAATACGT AGATACACTT ACTAACCGTG ATTTAAATAA TCATCAGGAA
    AAAAGTGAAG TGCACGTTGT TATTGAAAAG GCCGTTGCTC GATTAAAAAA AGAAGATCGA
    TTGCTTCCTC AAATCGGTCG TATGCGAGAA ATGCTTTCTC GTGGTTTAGC CGTTCATCAT
    GGCGGACTGT TACCCATCAT CAAGGAAATA GTAGAAATCC TTTTTCAAAG AGGGTTGGTG
    AAGGTTCTGT TTGCTACTGA GACCTTTGCA ATGGGTGTAA ATATGCCTGC AAAATCGGTG
    GTTTTTTCCG GTACACAAAA GCATGATGGA CGGAACTTCC GTGATCTTCT TCCCGGAGAG
    TATACACAAT GCTCTGGACG CGCTGGACGC CGTGGTCTTG ATGTCACTGG TACAGTTATT
    ATATTATCGC GTTCAGAACT TCCTGACACA GCTTCGTTGA GACACATGAT TATGGGGCCT
    TCGTCTAAGC TTATAAGTCA ATTTCGCTTA ACATATAATA TGATACTTAA CCTCCTACGT
    GTAGAGACGC TTAGAATTGA AGACATGATT AAACGCAGCT TTAGTGAAAA CGTTAACCAA
    ACATTGGTGC CTCAGCACGA GGAAAAAATA AAAAGCTTTG AAGAAAAGTT GTCGGCTTTA
    AAGAAGGAAA TGTCTGATGT TGATTTGAAA GAAATTAAAA GTTGTTTATT GAGCAGTGAA
    TCTTTTAAAG AGTATACCAA AAAAATGCAC TTCCGAGCAA TAACTACTGC AAATGGCAAA
    AGAATATTTA AAGATGGAAG AGTCATTGTT TTTCAACAAC TTGATTTTAC TCGCACTGTT
    GGAGTTCTAC TCGGAACATC CATTAGAACC AATGCTTCAG ACTGTACACT GGAAGTTGCA
    TATTTAAATC CACAGAACAA CCTCAAACGA CCATCGGATT TACTTGCTTT TGCTGACGCG
    TTCAATGATG TTTATGATAA CGCTATCTTT GATGAATCAA ACCAATTCAA GTATGGCCTT
    ATCAATCTTT CCGGTATTGA AAGAGTCTGC AATACGATCC TTCGAATTGA TTCTGGTGGA
    ATTAGAGACC GTCGAGGGGG GGCTTTTCGA AAACTTAGTG AACAATTTGC ATCAATTAAA
    AAGTTTTCCG ACCTGCTTTT TGAAGAAGTT AATTGGAGTA AAGTCAGAGA CTTTGAATTT
    TGTGAAGCTT TCGAAAAACG AAATTTTCTT CAGAACAAGT TATCCGGAAA TCCAATTATC
    TCGACTCCAA ACTTTCTTAC TCATTTCGCC TTAGCTTACC AAGAGTATGA ATTGGAAAGC
    AATATCGATA ACTTATCTTC CTATATTTCC GATCAAAACC TAGAGTTACT GCCTGATTAC
    GAACAACGGA TAAAAGTATT GCAAGAACTA GGATATATTG ACGCAGAGCG AACTGTTTTA
    CTAAAGGGAA GAGTCGCATG TGAAATTAAT TCCACCAGTG AATTGGTATT AACAGAGTTG
    ATTTTGGAAA ACAGTTTGGC GGACTTTTCT TGTGAAGAGA CAATAGCATT GTTGTCTGCA
    TTTGTTTTCG ATGAGAAAAC GGAGGTTGAA CCCACAATTT CTCCTCATTT ACAAAAAGGA
    AAGGAAATGA TACTATCAGT GGCCGAGAAA GTTAATCAAA TTCAAGAGCA CTATCAGGTA
    TTGTATTTTA ATGAAGGTAA TGATTTTGAA TCACAACCAC GATTTGGTTT AATGGAGGTT
    TGCTACGAGT GGGCTAGAGG GATGTCTTTC AACCGTATCA CAGACTTAAC AGACGTTTTA
    GAAGGATCGA TTGTAAGAAC AATTATAAGA CTTGATGAAG TTCTACGTGA ATGCAGAGGA
    GCAGCCCGTG TCGTCGGCGA TTCTTCCATG TATACGAAGA TGGAAGAGTG TCAAAATCTA
    ATTCGCAGAA ACATAGTCTT CTGCCCTTCA CTGTATATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_588095.1
    CDS21..3662
    Translation

    Target ORF information:

    RefSeq Version NM_001023086.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative Ski complex RNA helicase Ski2 (SPCC550.03c), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001023086.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTTCTA AACTTGTAGA TGCAATCAAC GAAGTAGCAG TTTCTAACAA GGATAAAATT 
    GAATTAGATG GAATAAAAGA TGATTCCTTT GACGGACACC TGAGTATCAC TGAAGCAACT
    TACGATGTTG ATACATTTTT GAAGCCTTCT CCAGCCTTAT CGAAGGACTG GATTCGAAAG
    TTGCAAAAAA AATGGGATAG GGAAATTACA TATAAAGGAC TATATGAGTA TCCCGAGACT
    TTAGCTCGTA CGCAAATTCG CTTTCAAAGA CATGGCCTGG AGGGTAAAAT TATGGGCTAC
    AAAGAGGTTC CTGAATTAAT AGAAGACCTT AATTCTAAAA ATTCGTCAAG TTTCTTACGA
    AAACCATCTT CGAAAAATGA ATTCGTTCGT GGAAGCACAA GCAACATTCC TTTTCTGGCA
    GATGACTCTG ATGTCGATGC TATAGCAGGC GAACCATCCG TGAAAATGGC TCTATACGGT
    GAAGATGGTT TGTTGCAAGT ACCGCCAGGG TTCTCCCGAG GGCTTTCCAT GACAGCGACA
    TCCACGACTG ACAATTTGAA CGATGAATTT GACCCCGAAA AATGGGATAC TAAGAAAGTG
    AAAAGCTCGA ATCGTAACTT TGTCACCATT CATGAGTTGA ATGAACACCT AAAAAATGTC
    AATTCTAAAC ATTCTGAAAT TGATGATCTA CTACCTGACA AAAGAAGCAT CGTTTCTCTA
    CCGCCTTCTA CTTTGAACTT GCATAAGCAA CCAGACTATG CTCATGTTGT AGATAGTAGT
    GCACCAATAG AAAATTTTCA ACAACTCGTT CCGGAAATGG CTCTTGACTT TCCTTTTGAA
    CTCGATAATT TTCAAAAGGA GGCTATTTAT CATCTTGAAA TGGGTGACTC TGTCTTTGTT
    GCTGCTCACA CCAGTGCTGG AAAAACGGTA GTAGCTGAAT ATGCAATTGC TTTGGCACAA
    AAACATATGA CGAAGGCTAT ATATACTTCA CCCATTAAAG CACTATCTAA TCAAAAGTTT
    AGAGATTTCA AACATAAATT TGAAGACGTT GGAATTTTAA CTGGTGATGT CCAAGTAAAT
    CCAGAAGGCT CATGCCTTCT TATGACAACA GAAATTTTAC GAAGCATGTT GTATAGAGGT
    GCTGACTTAA TTCGTGATGT TGAGTTTGTT ATCTTTGACG AAGTTCATTA TGTGAATGAT
    TTAGAAAGAG GTGTAGTTTG GGAGGAAGTT ATTATTATGC TTCCTCCTCA TGTAACTTTG
    ATTCTTTTAT CTGCTACCGT CCCTAACACC AAAGAGTTTG CTTCTTGGGT TGGACGAACA
    AAGAAAAAGA ACATTTATGT TATCTCAACT TTGAAGCGAC CAGTGCCCTT AGAGCATTAT
    CTCTGGGTGA AACAGAACAT GTTTAAAATC GTTGATCAGC ACGGTCGGTT TTTGATGGAT
    GGCTACAAAT CCGCAAACGA TGCACTGAAA AAGCCTGATA AACCTGTTAT AGCTAAGGAT
    AATAAGAACT CAGCTAGGGG TCGGGGTGCC GCTAGAGGCA GAGGCGTTCA AACGAATATG
    ATGCGTGGCA GAGGAAGCGC AAAGTCTGTT GAAAGAAGAG ATGCTAACAC TTGGGTTCAC
    TTAATTGGTC ATTTACATAA GCAAAATCTT TTGCCTGTAA TAGTATTTGT ATTTTCGAAG
    AAACGCTGTG AGGAATACGT AGATACACTT ACTAACCGTG ATTTAAATAA TCATCAGGAA
    AAAAGTGAAG TGCACGTTGT TATTGAAAAG GCCGTTGCTC GATTAAAAAA AGAAGATCGA
    TTGCTTCCTC AAATCGGTCG TATGCGAGAA ATGCTTTCTC GTGGTTTAGC CGTTCATCAT
    GGCGGACTGT TACCCATCAT CAAGGAAATA GTAGAAATCC TTTTTCAAAG AGGGTTGGTG
    AAGGTTCTGT TTGCTACTGA GACCTTTGCA ATGGGTGTAA ATATGCCTGC AAAATCGGTG
    GTTTTTTCCG GTACACAAAA GCATGATGGA CGGAACTTCC GTGATCTTCT TCCCGGAGAG
    TATACACAAT GCTCTGGACG CGCTGGACGC CGTGGTCTTG ATGTCACTGG TACAGTTATT
    ATATTATCGC GTTCAGAACT TCCTGACACA GCTTCGTTGA GACACATGAT TATGGGGCCT
    TCGTCTAAGC TTATAAGTCA ATTTCGCTTA ACATATAATA TGATACTTAA CCTCCTACGT
    GTAGAGACGC TTAGAATTGA AGACATGATT AAACGCAGCT TTAGTGAAAA CGTTAACCAA
    ACATTGGTGC CTCAGCACGA GGAAAAAATA AAAAGCTTTG AAGAAAAGTT GTCGGCTTTA
    AAGAAGGAAA TGTCTGATGT TGATTTGAAA GAAATTAAAA GTTGTTTATT GAGCAGTGAA
    TCTTTTAAAG AGTATACCAA AAAAATGCAC TTCCGAGCAA TAACTACTGC AAATGGCAAA
    AGAATATTTA AAGATGGAAG AGTCATTGTT TTTCAACAAC TTGATTTTAC TCGCACTGTT
    GGAGTTCTAC TCGGAACATC CATTAGAACC AATGCTTCAG ACTGTACACT GGAAGTTGCA
    TATTTAAATC CACAGAACAA CCTCAAACGA CCATCGGATT TACTTGCTTT TGCTGACGCG
    TTCAATGATG TTTATGATAA CGCTATCTTT GATGAATCAA ACCAATTCAA GTATGGCCTT
    ATCAATCTTT CCGGTATTGA AAGAGTCTGC AATACGATCC TTCGAATTGA TTCTGGTGGA
    ATTAGAGACC GTCGAGGGGG GGCTTTTCGA AAACTTAGTG AACAATTTGC ATCAATTAAA
    AAGTTTTCCG ACCTGCTTTT TGAAGAAGTT AATTGGAGTA AAGTCAGAGA CTTTGAATTT
    TGTGAAGCTT TCGAAAAACG AAATTTTCTT CAGAACAAGT TATCCGGAAA TCCAATTATC
    TCGACTCCAA ACTTTCTTAC TCATTTCGCC TTAGCTTACC AAGAGTATGA ATTGGAAAGC
    AATATCGATA ACTTATCTTC CTATATTTCC GATCAAAACC TAGAGTTACT GCCTGATTAC
    GAACAACGGA TAAAAGTATT GCAAGAACTA GGATATATTG ACGCAGAGCG AACTGTTTTA
    CTAAAGGGAA GAGTCGCATG TGAAATTAAT TCCACCAGTG AATTGGTATT AACAGAGTTG
    ATTTTGGAAA ACAGTTTGGC GGACTTTTCT TGTGAAGAGA CAATAGCATT GTTGTCTGCA
    TTTGTTTTCG ATGAGAAAAC GGAGGTTGAA CCCACAATTT CTCCTCATTT ACAAAAAGGA
    AAGGAAATGA TACTATCAGT GGCCGAGAAA GTTAATCAAA TTCAAGAGCA CTATCAGGTA
    TTGTATTTTA ATGAAGGTAA TGATTTTGAA TCACAACCAC GATTTGGTTT AATGGAGGTT
    TGCTACGAGT GGGCTAGAGG GATGTCTTTC AACCGTATCA CAGACTTAAC AGACGTTTTA
    GAAGGATCGA TTGTAAGAAC AATTATAAGA CTTGATGAAG TTCTACGTGA ATGCAGAGGA
    GCAGCCCGTG TCGTCGGCGA TTCTTCCATG TATACGAAGA TGGAAGAGTG TCAAAATCTA
    ATTCGCAGAA ACATAGTCTT CTGCCCTTCA CTGTATATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L