EBS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	EBS1
Entrez Gene ID	843462
Full Name	EMS-MUTAGENIZED BRI1 SUPPRESSOR 1
Synonyms	EMS-mutagenized bri1 suppressor 1,F3I17.13,F3I17_13,PRIORITY IN SWEET LIFE 2,PSL2,UDP-GLUCOSE:GLYCOPROTEIN GLUCOSYLTRANSFERASE,UGGT
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_105791.5	NP_177278.3	EMS-MUTAGENIZED BRI1 SUPPRESSOR 1
NM_001334490.1	NP_001323059.1	EMS-MUTAGENIZED BRI1 SUPPRESSOR 1
NM_001198441.1	NP_001185370.1	EMS-MUTAGENIZED BRI1 SUPPRESSOR 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UGGT1	XP_001141314.1
Drosophila melanogaster (fruit fly)	Ugt	NP_524151.2
	UGGT1	XP_001091373.1
Bos taurus (cattle)	UGGT1	XP_002685277.1
Gallus gallus (chicken)	UGGT1	XP_004937020.1
Danio rerio (zebrafish)	uggt1	NP_001071002.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003560	XP_313307.5
Caenorhabditis elegans (roundworm)	uggt-1	NP_509268.1
Arabidopsis thaliana (thale cress)	EBS1	NP_001185370.1
Schizosaccharomyces pombe (fission yeast)	gpt1	NP_595281.1
Magnaporthe oryzae (rice blast fungus)	MGG_03510	XP_003716409.1
Canis lupus familiaris (dog)	UGGT1	XP_005631915.1
Mus musculus (house mouse)	Uggt1	NP_942602.2
Neurospora crassa	NCU02349	XP_959471.1
Homo sapiens (human)	UGGT1	NP_064505.1
Rattus norvegicus (Norway rat)	Uggt1	NP_598280.1
Xenopus tropicalis (tropical clawed frog)	uggt1	XP_004914541.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a lack of UGGT activity alters plant vegetative development and impairs the response to several abiotic and biotic stresses.
Title: The UDP-glucose: glycoprotein glucosyltransferase (UGGT), a key enzyme in ER quality control, plays a significant role in plant growth as well as biotic and abiotic stress in Arabidopsis thaliana.

a missense mutation in the Arabidopsis EMS-mutagenized bri1 suppressor 3 (EBS3) gene suppresses a dwarf mutant, bri1-9, the phenotypes of which are caused by ER retention and ERAD of a brassinosteroid receptor, BRASSINOSTEROID-INSENSITIVE 1 (BR1)
Title: Evolutionarily conserved glycan signal to degrade aberrant brassinosteroid receptors in Arabidopsis.

The following EBS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EBS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb35177	NM_001334490.1 Latest version!	Arabidopsis thaliana EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 (EBS1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAb27647	NM_105791.5 Latest version!	Arabidopsis thaliana EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 (EBS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OAb29487	NM_001198441.1 Latest version!	Arabidopsis thaliana EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 (EBS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EBS1 ( NM_001334490.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001323059.1 Arabidopsis thaliana EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 (EBS1), partial mRNA.

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CloneID	OAb35177
Clone ID Related Accession (Same CDS sequence)	NM_001334490.1
Accession Version	NM_001334490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	EMS-MUTAGENIZED BRI1 SUPPRESSOR 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). COMPLETENESS: incomplete on the 5' end.

ATGTCGTGGC TTTTGTACGT TATTTACGAA AATGTCCTTT TAGTGTTAGT AAGGATATTT 
CGATTCCCGG CTCGTTCGTT ACTGGTTTTT TTCTTCTTCT CCGTCGCGGA AAAGATGGGG 
ACCACCACCA ATCTGAGATC TTGGCTGTAT CTCATTCTTC TCTTCATCGT CGTCGTCGGC 
GTCAACGCTC AGAATCGGAG ACCTAAGAAT GTTCAGGTGG CTGTTAAGGC TAAGTGGCAG 
GGTACTCCTC TTCTCCTTGA GGCTGGTGAG TTGATTTCCA AAGAATCGAA GCAGTTGTTC 
TGGGAGTTTA CTGATGCTTG GCTTGGTTCC GATGGAGATG ATTCCGATTG TAAATCAGCT 
AGAGATTGTC TTCTTAAGAT ATCTAAACAG GCTTCGACTC TGTTAGCTCA ACCAGTGGCT 
TCTCTCTTCC ATTTCTCACT TACTCTGAGA TCTGCTTCGC CTAGGCTTGT GCTTTACCGT 
CAATTGGCTG ATGAGTCTCT ATCTTCTTTC CCTCATGGTG ATGATCCCTC TGCGACTGGC 
TGCTGTTGGG TTGATACTGG CTCCTCTCTG TTTTACGATG TTGCAGATCT TCAATCCTGG 
CTTGCCTCTG CTCCTGCTGT TGGAGATGCT GTTCAGGGGC CTGAGCTATT CGACTTCGAT 
CATGTTCATT TTGATTCAAG AGCTGGAAGC CCAGTTGCTG TTCTCTATGG GGCTGTTGGG 
ACTGATTGCT TTAGGAAGTT TCACCTGTCG CTTGCTAAAG CAGCCAAGGA GGGAAAAGTT 
ACATATGTTG TTAGACCAGT ATTACCTTTG GGTTGTGAAG GGAAAACTAG ACCCTGCGGT 
GCCATTGGAG CAAGGGATAA TGTTAGCTTA GCTGGTTATG GTGTGGAGCT TGCTTTGAAG 
AACATGGAGT ACAAGGCTAT GGATGATAGT GCTATAAAGA AAGGTATCAC TCTTGAAGAT 
CCTAGGACTG AAGATCTTAG TCAAGATGTT AGGGGCTTTA TATTCTCAAA AATCTTGGAC 
AGGAAACCCG AGCTAAGATC AGAAGTCATG GCTTTTAGGG ATTATCTCCT ATCATCAACA 
GTATCAGACA CGCTTGATGT TTGGGAGCTG AAAGATTTAG GACACCAAAC AGCGCAGAGA 
ATAGTCCATG CCTCTGATCC TCTGCAGTCT ATGCAAGAAA TTAATCAAAA CTTTCCTAGT 
GTAGTTTCTT CATTATCTCG CATGAAGCTC AATGAATCAA TCAAAGACGA GATACTTTCA 
AACCAGAGAA TGGTTCCCCC TGGCAAGGCT TTGTTGGCTC TGAATGGTGC CCTGTTGAAC 
ATCGAAGATA TTGATCTATA CATGTTGATG GATTTGGCTC ATCAGGAATT GTCCCTGGCC 
AACCATTTTT CGAAGCTTAA GATACCTGAT GGAGCCATAA GGAAACTTCT ACTAACTACC 
CCTCTCCCTG AACCGGATTC ATATCGGGTT GATTTCCGAT CCGTGCATGT TACCTATCTC 
AACAATCTGG AAGAGGATGA TATGTACAAG CGTTGGCGGA GTAATATTAA CGAGATTTTG 
ATGCCTGCCT TCCCTGGACA ACTACGTTAC ATCCGAAAGA ACCTATTCCA TGCAGTTTAC 
GTCATTGATC CAGCGACTGC CTGTGGTCTT GAGTCTATTG AAACTCTCAG ATCTTTATAT 
GAAAATCAAC TCCCTGTGAG ATTTGGGGTG ATATTATATT CAACTCAACT AATCAAGACA 
ATCGAGAATA ATGGTGGTCA AATCCCATCT TCTGATGCAG TGACTAATGC CCAAGTTAAA 
GAGGACTTAT CGACTATGGT CATACGTCTT TTTCTGTACA TCAAGGAGCA CCATGGGATT 
CAAACAGCTT TTCAGTTCTT GGGCAATTTA AACACACTAA GGACTGAATC TGCTGATTCT 
TCCGAAGCAG ACATTGAACA GGAGCACGTT GATGGTGCAT TTGTTGAGAC AATTTTACCA 
AAGGTTAAAA CACTCCCTCA GGATATATTG CTGAAACTGC GACAAGAGCA CACTTTGAAG 
GAAGCATCTG AAGCAAGCTC CATGTTTGTA TTTAAGTTGG GTCTGGCGAA ATTGAAGTGT 
TCCTTTTTGA TGAATGGCCT TGTCTTTGAT TCTGTTGAGG AAGAAACTCT TTTAAATGCC 
ATGAATGAAG AGCTTCCCAA GATACAAGAA CAAGTTTATT ACGGCCAGAT AGAATCCCAT 
ACTAAGGTTT TGGACAAGTT GCTTTCGGAA AGTGGTCTTA GCCGTTATAA TCCACAGATT 
ATTAGTGGAG GAAAGAACAA ACCAAGGTTT GTGTCGCTAG CTTCATCTAC CAGGAAAGGG 
GAATCTATGT TGAATGATGT TAACTACTTG CATTCTCCTG AAACTTCAGA GGATGTGAAG 
TATGTGACTC ATCTCCTTGC TGCTGATGTT GCAACGAAGA AAGGAATGAA ATTGTTACAT 
GAAGGAGTCC GTTACCTGAT TGGAGGTTCC AAAAGTGCTC GTCTTGGAGT CTTATTTAGT 
AGCAGCCAGA ATGCTGACCC ACATAGCCTT CTATTTATAA AGTTTTTCGA AAAAACTGCT 
TCATCGTTTA GTCATAAGGA AAAGGTGCTA TACTTCTTGG ATAAACTATG CTTGTTTTAC 
GAGCGTGAAT ATCTGTTAAA AACATCAGTG GAATCAGCTA GCTCTCAAAT GTTTATTGAT 
AAAGTCCTTG AGCTTGCTGA TGAGTATGGG CTATCTTCTA AGGCATACCG ATCTTGTCTC 
GTTGAATCAG TGGATGAAGA ATTACTTAAG CGTTTGACAA AGGTCGCCCA ATTCTTGTCT 
TGGGAACTTG GACTTGAATC TGATGCAAAT GCAATAATTT CAAATGGAAG GGTGATATTT 
CCTGTGGATG AGAGGACATT TTTGGGCCAA GATTTACATC TTCTTGAGTC AATGGAGTTC 
AATCAAAGAG TAAAGCCTGT TCAAGAAATT ATTGAAGGCA TTGAATGGCA AGATGTGGAT 
CCCGATCTGC TGACAAGTAA ATATTTTAGC GATGTCTTCA TGTTTGTGTC ATCTGCAATG 
GCAACACGGG ATCGTAGTTC TGAAAGTGCG CGGTTTGAGG TGTTGAATTC AGAATACAGT 
GCGGTTTTGC TGGGAAATGA AAATGCAACG ATACACATTG ATGCTGTTAT CGATCCTTTA 
AGCCCTACTG GTCAAAAACT AGCATCACTT CTTCAAGTTC TACAGAAACA TGTTCAGACG 
AGCATGAGGA TTGTTCTGAA TCCCATGAGT TCCCTTGTGG ACATTCCTCT GAAAAATTAC 
TACAGATATG TCTTACCTAA TACGGACGAT TACAGCAGTA CAGGCTTTGA CGTAGATGGG 
CCTAAGGCAT TTTTCGCAAA TATGCCATTG TCTAAGACAC TTACCATGAA TCTTGACGTT 
CCAGAGCCAT GGCTGGTTGA ACCAGTAATT GCCATTCATG ATCTTGATAA TATATTGCTT 
GAGAATCTAG GAGATACCAC TACACTGCAG GCAGTTTTTG AGGTTGAATC TCTTGTTCTT 
ACAGGTCATT GCGCGGAAAA AGATCATGAG GCTCCTCGTG GCCTTCAGCT GATTCTGGGA 
ACCAAAAATA GACCGCATTT GGTTGATACC CTTGTAATGG CCAATTTGGG TTATTGGCAG 
ATGAAAGTAT CCCCAGGGGT TTGGTATTTG CAACTTGCTC CGGGTAGAAG TTCGGAGCTA 
TACGCTTTGA AAGGAGGAAA TGATGGGAGT CAAGATCAAT CCTCGCTGAA ACGTATAACT 
ATCGATGATC TGCGTGGTAA AGTTGTTCAT CTAGAAGTAG TTAAGAGAAA GGGTAAGGAG 
CATGAAAAGT TGCTAGTTCC TTCAGACGGT GATGATGCTG TGCAACAAAA CAAGGAAGGA 
AGCTGGAACT CAAATTTCTT GAAATGGGCG TCTGGTTTTG TTGGTGGTCG TCAACAATCA 
ATGAAGGGAG GTCCTGACAA AGAGCATGAG AAGGGTGGGC GACAAGGGAA AACGATAAAT 
ATATTCTCCA TTGCTTCAGG GCATTTATAC GAGCGTTTCC TTAAGATTAT GATTCTCAGT 
GTCCTGAAGA ACACCAATCG TCCTGTAAAA TTCTGGTTTA TTAAGAATTA TCTCTCTCCT 
CAATTTAAGG ATGTGATTCC ACATATGGCA CAAGAATACA ACTTTGAGTA TGAGCTGATT 
ACATACAAAT GGCCGTCCTG GTTGCATAAA CAGAAAGAAA AGCAACGAAT TATTTGGGCC 
TACAAAATTC TTTTCCTTGA TGTCATATTT CCTCTCTCAT TGGAAAAGGT TATATTTGTC 
GACGCAGATC AAATTATAAG GACAGACATG GGAGAACTGT ATGATATGGA TATTAAAGGA 
AGACCTCTCG CGTATACTCC TTTTTGTGAC AACAACAGGG AAATGGATGG CTATAAATTT 
TGGAAACAGG GTTTCTGGAA AGAACATTTA CGTGGTAGGC CATACCATAT CAGTGCTCTA 
TACGTTGTTG ACTTGGTTAA GTTCCGAGAG ACAGCGGCAG GAGATAATCT AAGAGTATTC 
TATGAGACGC TTAGCAAGGA TCCAAACAGT TTGTCCAACC TAGATCAGGA TCTTCCTAAT 
TATGCTCAGC ATACTGTTCC TATATTTTCA CTTCCCCAAG AGTGGTTGTG GTGTGAATCC 
TGGTGTGGAA ACGCCACAAA AGCCAAGGCC AGAACAATCG ATCTTTGCAA TAATCCCATG 
ACAAAGGAAC CAAAGCTTCA GGGTGCCCGA AGAATCGTAA CCGAATGGCC CGATCTTGAT 
CTCGAAGCAC GAAAATTCAC AGCAAAAATC TTGGGTGAAG ATGTGGAACT CAACGAGCCA 
GTAGCTGCTC CTGCTACAGA CAAGCCTAAT CCTCTTCCAT CAAATGACAT CTCTGAAGAT 
ACAGAACAAG ACTTGGAATC TAAAGCAGAA CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001323059.1
CDS	1..4956
Translation	MSWLLYVIYENVLLVLVRIFRFPARSLLVFFFFSVAEKMGTTTNLRSWLYLILLFIVVVGVNAQNRRPKNVQVAVKAKWQGTPLLLEAGELISKESKQLFWEFTDAWLGSDGDDSDCKSARDCLLKISKQASTLLAQPVASLFHFSLTLRSASPRLVLYRQLADESLSSFPHGDDPSATGCCWVDTGSSLFYDVADLQSWLASAPAVGDAVQGPELFDFDHVHFDSRAGSPVAVLYGAVGTDCFRKFHLSLAKAAKEGKVTYVVRPVLPLGCEGKTRPCGAIGARDNVSLAGYGVELALKNMEYKAMDDSAIKKGITLEDPRTEDLSQDVRGFIFSKILDRKPELRSEVMAFRDYLLSSTVSDTLDVWELKDLGHQTAQRIVHASDPLQSMQEINQNFPSVVSSLSRMKLNESIKDEILSNQRMVPPGKALLALNGALLNIEDIDLYMLMDLAHQELSLANHFSKLKIPDGAIRKLLLTTPLPEPDSYRVDFRSVHVTYLNNLEEDDMYKRWRSNINEILMPAFPGQLRYIRKNLFHAVYVIDPATACGLESIETLRSLYENQLPVRFGVILYSTQLIKTIENNGGQIPSSDAVTNAQVKEDLSTMVIRLFLYIKEHHGIQTAFQFLGNLNTLRTESADSSEADIEQEHVDGAFVETILPKVKTLPQDILLKLRQEHTLKEASEASSMFVFKLGLAKLKCSFLMNGLVFDSVEEETLLNAMNEELPKIQEQVYYGQIESHTKVLDKLLSESGLSRYNPQIISGGKNKPRFVSLASSTRKGESMLNDVNYLHSPETSEDVKYVTHLLAADVATKKGMKLLHEGVRYLIGGSKSARLGVLFSSSQNADPHSLLFIKFFEKTASSFSHKEKVLYFLDKLCLFYEREYLLKTSVESASSQMFIDKVLELADEYGLSSKAYRSCLVESVDEELLKRLTKVAQFLSWELGLESDANAIISNGRVIFPVDERTFLGQDLHLLESMEFNQRVKPVQEIIEGIEWQDVDPDLLTSKYFSDVFMFVSSAMATRDRSSESARFEVLNSEYSAVLLGNENATIHIDAVIDPLSPTGQKLASLLQVLQKHVQTSMRIVLNPMSSLVDIPLKNYYRYVLPNTDDYSSTGFDVDGPKAFFANMPLSKTLTMNLDVPEPWLVEPVIAIHDLDNILLENLGDTTTLQAVFEVESLVLTGHCAEKDHEAPRGLQLILGTKNRPHLVDTLVMANLGYWQMKVSPGVWYLQLAPGRSSELYALKGGNDGSQDQSSLKRITIDDLRGKVVHLEVVKRKGKEHEKLLVPSDGDDAVQQNKEGSWNSNFLKWASGFVGGRQQSMKGGPDKEHEKGGRQGKTINIFSIASGHLYERFLKIMILSVLKNTNRPVKFWFIKNYLSPQFKDVIPHMAQEYNFEYELITYKWPSWLHKQKEKQRIIWAYKILFLDVIFPLSLEKVIFVDADQIIRTDMGELYDMDIKGRPLAYTPFCDNNREMDGYKFWKQGFWKEHLRGRPYHISALYVVDLVKFRETAAGDNLRVFYETLSKDPNSLSNLDQDLPNYAQHTVPIFSLPQEWLWCESWCGNATKAKARTIDLCNNPMTKEPKLQGARRIVTEWPDLDLEARKFTAKILGEDVELNEPVAAPATDKPNPLPSNDISEDTEQDLESKAEL

Target ORF information:

RefSeq Version	NM_001334490.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 (EBS1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001334490.1

ORF Insert Sequence:

ATGTCGTGGC TTTTGTACGT TATTTACGAA AATGTCCTTT TAGTGTTAGT AAGGATATTT 
CGATTCCCGG CTCGTTCGTT ACTGGTTTTT TTCTTCTTCT CCGTCGCGGA AAAGATGGGG 
ACCACCACCA ATCTGAGATC TTGGCTGTAT CTCATTCTTC TCTTCATCGT CGTCGTCGGC 
GTCAACGCTC AGAATCGGAG ACCTAAGAAT GTTCAGGTGG CTGTTAAGGC TAAGTGGCAG 
GGTACTCCTC TTCTCCTTGA GGCTGGTGAG TTGATTTCCA AAGAATCGAA GCAGTTGTTC 
TGGGAGTTTA CTGATGCTTG GCTTGGTTCC GATGGAGATG ATTCCGATTG TAAATCAGCT 
AGAGATTGTC TTCTTAAGAT ATCTAAACAG GCTTCGACTC TGTTAGCTCA ACCAGTGGCT 
TCTCTCTTCC ATTTCTCACT TACTCTGAGA TCTGCTTCGC CTAGGCTTGT GCTTTACCGT 
CAATTGGCTG ATGAGTCTCT ATCTTCTTTC CCTCATGGTG ATGATCCCTC TGCGACTGGC 
TGCTGTTGGG TTGATACTGG CTCCTCTCTG TTTTACGATG TTGCAGATCT TCAATCCTGG 
CTTGCCTCTG CTCCTGCTGT TGGAGATGCT GTTCAGGGGC CTGAGCTATT CGACTTCGAT 
CATGTTCATT TTGATTCAAG AGCTGGAAGC CCAGTTGCTG TTCTCTATGG GGCTGTTGGG 
ACTGATTGCT TTAGGAAGTT TCACCTGTCG CTTGCTAAAG CAGCCAAGGA GGGAAAAGTT 
ACATATGTTG TTAGACCAGT ATTACCTTTG GGTTGTGAAG GGAAAACTAG ACCCTGCGGT 
GCCATTGGAG CAAGGGATAA TGTTAGCTTA GCTGGTTATG GTGTGGAGCT TGCTTTGAAG 
AACATGGAGT ACAAGGCTAT GGATGATAGT GCTATAAAGA AAGGTATCAC TCTTGAAGAT 
CCTAGGACTG AAGATCTTAG TCAAGATGTT AGGGGCTTTA TATTCTCAAA AATCTTGGAC 
AGGAAACCCG AGCTAAGATC AGAAGTCATG GCTTTTAGGG ATTATCTCCT ATCATCAACA 
GTATCAGACA CGCTTGATGT TTGGGAGCTG AAAGATTTAG GACACCAAAC AGCGCAGAGA 
ATAGTCCATG CCTCTGATCC TCTGCAGTCT ATGCAAGAAA TTAATCAAAA CTTTCCTAGT 
GTAGTTTCTT CATTATCTCG CATGAAGCTC AATGAATCAA TCAAAGACGA GATACTTTCA 
AACCAGAGAA TGGTTCCCCC TGGCAAGGCT TTGTTGGCTC TGAATGGTGC CCTGTTGAAC 
ATCGAAGATA TTGATCTATA CATGTTGATG GATTTGGCTC ATCAGGAATT GTCCCTGGCC 
AACCATTTTT CGAAGCTTAA GATACCTGAT GGAGCCATAA GGAAACTTCT ACTAACTACC 
CCTCTCCCTG AACCGGATTC ATATCGGGTT GATTTCCGAT CCGTGCATGT TACCTATCTC 
AACAATCTGG AAGAGGATGA TATGTACAAG CGTTGGCGGA GTAATATTAA CGAGATTTTG 
ATGCCTGCCT TCCCTGGACA ACTACGTTAC ATCCGAAAGA ACCTATTCCA TGCAGTTTAC 
GTCATTGATC CAGCGACTGC CTGTGGTCTT GAGTCTATTG AAACTCTCAG ATCTTTATAT 
GAAAATCAAC TCCCTGTGAG ATTTGGGGTG ATATTATATT CAACTCAACT AATCAAGACA 
ATCGAGAATA ATGGTGGTCA AATCCCATCT TCTGATGCAG TGACTAATGC CCAAGTTAAA 
GAGGACTTAT CGACTATGGT CATACGTCTT TTTCTGTACA TCAAGGAGCA CCATGGGATT 
CAAACAGCTT TTCAGTTCTT GGGCAATTTA AACACACTAA GGACTGAATC TGCTGATTCT 
TCCGAAGCAG ACATTGAACA GGAGCACGTT GATGGTGCAT TTGTTGAGAC AATTTTACCA 
AAGGTTAAAA CACTCCCTCA GGATATATTG CTGAAACTGC GACAAGAGCA CACTTTGAAG 
GAAGCATCTG AAGCAAGCTC CATGTTTGTA TTTAAGTTGG GTCTGGCGAA ATTGAAGTGT 
TCCTTTTTGA TGAATGGCCT TGTCTTTGAT TCTGTTGAGG AAGAAACTCT TTTAAATGCC 
ATGAATGAAG AGCTTCCCAA GATACAAGAA CAAGTTTATT ACGGCCAGAT AGAATCCCAT 
ACTAAGGTTT TGGACAAGTT GCTTTCGGAA AGTGGTCTTA GCCGTTATAA TCCACAGATT 
ATTAGTGGAG GAAAGAACAA ACCAAGGTTT GTGTCGCTAG CTTCATCTAC CAGGAAAGGG 
GAATCTATGT TGAATGATGT TAACTACTTG CATTCTCCTG AAACTTCAGA GGATGTGAAG 
TATGTGACTC ATCTCCTTGC TGCTGATGTT GCAACGAAGA AAGGAATGAA ATTGTTACAT 
GAAGGAGTCC GTTACCTGAT TGGAGGTTCC AAAAGTGCTC GTCTTGGAGT CTTATTTAGT 
AGCAGCCAGA ATGCTGACCC ACATAGCCTT CTATTTATAA AGTTTTTCGA AAAAACTGCT 
TCATCGTTTA GTCATAAGGA AAAGGTGCTA TACTTCTTGG ATAAACTATG CTTGTTTTAC 
GAGCGTGAAT ATCTGTTAAA AACATCAGTG GAATCAGCTA GCTCTCAAAT GTTTATTGAT 
AAAGTCCTTG AGCTTGCTGA TGAGTATGGG CTATCTTCTA AGGCATACCG ATCTTGTCTC 
GTTGAATCAG TGGATGAAGA ATTACTTAAG CGTTTGACAA AGGTCGCCCA ATTCTTGTCT 
TGGGAACTTG GACTTGAATC TGATGCAAAT GCAATAATTT CAAATGGAAG GGTGATATTT 
CCTGTGGATG AGAGGACATT TTTGGGCCAA GATTTACATC TTCTTGAGTC AATGGAGTTC 
AATCAAAGAG TAAAGCCTGT TCAAGAAATT ATTGAAGGCA TTGAATGGCA AGATGTGGAT 
CCCGATCTGC TGACAAGTAA ATATTTTAGC GATGTCTTCA TGTTTGTGTC ATCTGCAATG 
GCAACACGGG ATCGTAGTTC TGAAAGTGCG CGGTTTGAGG TGTTGAATTC AGAATACAGT 
GCGGTTTTGC TGGGAAATGA AAATGCAACG ATACACATTG ATGCTGTTAT CGATCCTTTA 
AGCCCTACTG GTCAAAAACT AGCATCACTT CTTCAAGTTC TACAGAAACA TGTTCAGACG 
AGCATGAGGA TTGTTCTGAA TCCCATGAGT TCCCTTGTGG ACATTCCTCT GAAAAATTAC 
TACAGATATG TCTTACCTAA TACGGACGAT TACAGCAGTA CAGGCTTTGA CGTAGATGGG 
CCTAAGGCAT TTTTCGCAAA TATGCCATTG TCTAAGACAC TTACCATGAA TCTTGACGTT 
CCAGAGCCAT GGCTGGTTGA ACCAGTAATT GCCATTCATG ATCTTGATAA TATATTGCTT 
GAGAATCTAG GAGATACCAC TACACTGCAG GCAGTTTTTG AGGTTGAATC TCTTGTTCTT 
ACAGGTCATT GCGCGGAAAA AGATCATGAG GCTCCTCGTG GCCTTCAGCT GATTCTGGGA 
ACCAAAAATA GACCGCATTT GGTTGATACC CTTGTAATGG CCAATTTGGG TTATTGGCAG 
ATGAAAGTAT CCCCAGGGGT TTGGTATTTG CAACTTGCTC CGGGTAGAAG TTCGGAGCTA 
TACGCTTTGA AAGGAGGAAA TGATGGGAGT CAAGATCAAT CCTCGCTGAA ACGTATAACT 
ATCGATGATC TGCGTGGTAA AGTTGTTCAT CTAGAAGTAG TTAAGAGAAA GGGTAAGGAG 
CATGAAAAGT TGCTAGTTCC TTCAGACGGT GATGATGCTG TGCAACAAAA CAAGGAAGGA 
AGCTGGAACT CAAATTTCTT GAAATGGGCG TCTGGTTTTG TTGGTGGTCG TCAACAATCA 
ATGAAGGGAG GTCCTGACAA AGAGCATGAG AAGGGTGGGC GACAAGGGAA AACGATAAAT 
ATATTCTCCA TTGCTTCAGG GCATTTATAC GAGCGTTTCC TTAAGATTAT GATTCTCAGT 
GTCCTGAAGA ACACCAATCG TCCTGTAAAA TTCTGGTTTA TTAAGAATTA TCTCTCTCCT 
CAATTTAAGG ATGTGATTCC ACATATGGCA CAAGAATACA ACTTTGAGTA TGAGCTGATT 
ACATACAAAT GGCCGTCCTG GTTGCATAAA CAGAAAGAAA AGCAACGAAT TATTTGGGCC 
TACAAAATTC TTTTCCTTGA TGTCATATTT CCTCTCTCAT TGGAAAAGGT TATATTTGTC 
GACGCAGATC AAATTATAAG GACAGACATG GGAGAACTGT ATGATATGGA TATTAAAGGA 
AGACCTCTCG CGTATACTCC TTTTTGTGAC AACAACAGGG AAATGGATGG CTATAAATTT 
TGGAAACAGG GTTTCTGGAA AGAACATTTA CGTGGTAGGC CATACCATAT CAGTGCTCTA 
TACGTTGTTG ACTTGGTTAA GTTCCGAGAG ACAGCGGCAG GAGATAATCT AAGAGTATTC 
TATGAGACGC TTAGCAAGGA TCCAAACAGT TTGTCCAACC TAGATCAGGA TCTTCCTAAT 
TATGCTCAGC ATACTGTTCC TATATTTTCA CTTCCCCAAG AGTGGTTGTG GTGTGAATCC 
TGGTGTGGAA ACGCCACAAA AGCCAAGGCC AGAACAATCG ATCTTTGCAA TAATCCCATG 
ACAAAGGAAC CAAAGCTTCA GGGTGCCCGA AGAATCGTAA CCGAATGGCC CGATCTTGAT 
CTCGAAGCAC GAAAATTCAC AGCAAAAATC TTGGGTGAAG ATGTGGAACT CAACGAGCCA 
GTAGCTGCTC CTGCTACAGA CAAGCCTAAT CCTCTTCCAT CAAATGACAT CTCTGAAGAT 
ACAGAACAAG ACTTGGAATC TAAAGCAGAA CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EBS1 ( NM_105791.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_177278.3 Arabidopsis thaliana EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 (EBS1), mRNA.

Price & Availability↑

CloneID	OAb27647
Clone ID Related Accession (Same CDS sequence)	NM_105791.5
Accession Version	NM_105791.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	EMS-MUTAGENIZED BRI1 SUPPRESSOR 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_105791.4.

ATGGGGACCA CCACCAATCT GAGATCTTGG CTGTATCTCA TTCTTCTCTT CATCGTCGTC 
GTCGGCGTCA ACGCTCAGAA TCGGAGACCT AAGAATGTTC AGGTGGCTGT TAAGGCTAAG 
TGGCAGGGTA CTCCTCTTCT CCTTGAGGCT GGTGAGTTGA TTTCCAAAGA ATCGAAGCAG 
TTGTTCTGGG AGTTTACTGA TGCTTGGCTT GGTTCCGATG GAGATGATTC CGATTGTAAA 
TCAGCTAGAG ATTGTCTTCT TAAGATATCT AAACAGGCTT CGACTCTGTT AGCTCAACCA 
GTGGCTTCTC TCTTCCATTT CTCACTTACT CTGAGATCTG CTTCGCCTAG GCTTGTGCTT 
TACCGTCAAT TGGCTGATGA GTCTCTATCT TCTTTCCCTC ATGGTGATGA TCCCTCTGCG 
ACTGGCTGCT GTTGGGTTGA TACTGGCTCC TCTCTGTTTT ACGATGTTGC AGATCTTCAA 
TCCTGGCTTG CCTCTGCTCC TGCTGTTGGA GATGCTGTTC AGGGGCCTGA GCTATTCGAC 
TTCGATCATG TTCATTTTGA TTCAAGAGCT GGAAGCCCAG TTGCTGTTCT CTATGGGGCT 
GTTGGGACTG ATTGCTTTAG GAAGTTTCAC CTGTCGCTTG CTAAAGCAGC CAAGGAGGGA 
AAAGTTACAT ATGTTGTTAG ACCAGTATTA CCTTTGGGTT GTGAAGGGAA AACTAGACCC 
TGCGGTGCCA TTGGAGCAAG GGATAATGTT AGCTTAGCTG GTTATGGTGT GGAGCTTGCT 
TTGAAGAACA TGGAGTACAA GGCTATGGAT GATAGTGCTA TAAAGAAAGG TATCACTCTT 
GAAGATCCTA GGACTGAAGA TCTTAGTCAA GATGTTAGGG GCTTTATATT CTCAAAAATC 
TTGGACAGGA AACCCGAGCT AAGATCAGAA GTCATGGCTT TTAGGGATTA TCTCCTATCA 
TCAACAGTAT CAGACACGCT TGATGTTTGG GAGCTGAAAG ATTTAGGACA CCAAACAGCG 
CAGAGAATAG TCCATGCCTC TGATCCTCTG CAGTCTATGC AAGAAATTAA TCAAAACTTT 
CCTAGTGTAG TTTCTTCATT ATCTCGCATG AAGCTCAATG AATCAATCAA AGACGAGATA 
CTTTCAAACC AGAGAATGGT TCCCCCTGGC AAGGCTTTGT TGGCTCTGAA TGGTGCCCTG 
TTGAACATCG AAGATATTGA TCTATACATG TTGATGGATT TGGCTCATCA GGAATTGTCC 
CTGGCCAACC ATTTTTCGAA GCTTAAGATA CCTGATGGAG CCATAAGGAA ACTTCTACTA 
ACTACCCCTC TCCCTGAACC GGATTCATAT CGGGTTGATT TCCGATCCGT GCATGTTACC 
TATCTCAACA ATCTGGAAGA GGATGATATG TACAAGCGTT GGCGGAGTAA TATTAACGAG 
ATTTTGATGC CTGCCTTCCC TGGACAACTA CGTTACATCC GAAAGAACCT ATTCCATGCA 
GTTTACGTCA TTGATCCAGC GACTGCCTGT GGTCTTGAGT CTATTGAAAC TCTCAGATCT 
TTATATGAAA ATCAACTCCC TGTGAGATTT GGGGTGATAT TATATTCAAC TCAACTAATC 
AAGACAATCG AGAATAATGG TGGTCAAATC CCATCTTCTG ATGCAGTGAC TAATGCCCAA 
GTTAAAGAGG ACTTATCGAC TATGGTCATA CGTCTTTTTC TGTACATCAA GGAGCACCAT 
GGGATTCAAA CAGCTTTTCA GTTCTTGGGC AATTTAAACA CACTAAGGAC TGAATCTGCT 
GATTCTTCCG AAGCAGACAT TGAACAGGAG CACGTTGATG GTGCATTTGT TGAGACAATT 
TTACCAAAGG TTAAAACACT CCCTCAGGAT ATATTGCTGA AACTGCGACA AGAGCACACT 
TTGAAGGAAG CATCTGAAGC AAGCTCCATG TTTGTATTTA AGTTGGGTCT GGCGAAATTG 
AAGTGTTCCT TTTTGATGAA TGGCCTTGTC TTTGATTCTG TTGAGGAAGA AACTCTTTTA 
AATGCCATGA ATGAAGAGCT TCCCAAGATA CAAGAACAAG TTTATTACGG CCAGATAGAA 
TCCCATACTA AGGTTTTGGA CAAGTTGCTT TCGGAAAGTG GTCTTAGCCG TTATAATCCA 
CAGATTATTA GTGGAGGAAA GAACAAACCA AGGTTTGTGT CGCTAGCTTC ATCTACCAGG 
AAAGGGGAAT CTATGTTGAA TGATGTTAAC TACTTGCATT CTCCTGAAAC TTCAGAGGAT 
GTGAAGTATG TGACTCATCT CCTTGCTGCT GATGTTGCAA CGAAGAAAGG AATGAAATTG 
TTACATGAAG GAGTCCGTTA CCTGATTGGA GGTTCCAAAA GTGCTCGTCT TGGAGTCTTA 
TTTAGTAGCA GCCAGAATGC TGACCCACAT AGCCTTCTAT TTATAAAGTT TTTCGAAAAA 
ACTGCTTCAT CGTTTAGTCA TAAGGAAAAG GTGCTATACT TCTTGGATAA ACTATGCTTG 
TTTTACGAGC GTGAATATCT GTTAAAAACA TCAGTGGAAT CAGCTAGCTC TCAAATGTTT 
ATTGATAAAG TCCTTGAGCT TGCTGATGAG TATGGGCTAT CTTCTAAGGC ATACCGATCT 
TGTCTCGTTG AATCAGTGGA TGAAGAATTA CTTAAGCGTT TGACAAAGGT CGCCCAATTC 
TTGTCTTGGG AACTTGGACT TGAATCTGAT GCAAATGCAA TAATTTCAAA TGGAAGGGTG 
ATATTTCCTG TGGATGAGAG GACATTTTTG GGCCAAGATT TACATCTTCT TGAGTCAATG 
GAGTTCAATC AAAGAGTAAA GCCTGTTCAA GAAATTATTG AAGGCATTGA ATGGCAAGAT 
GTGGATCCCG ATCTGCTGAC AAGTAAATAT TTTAGCGATG TCTTCATGTT TGTGTCATCT 
GCAATGGCAA CACGGGATCG TAGTTCTGAA AGTGCGCGGT TTGAGGTGTT GAATTCAGAA 
TACAGTGCGG TTTTGCTGGG AAATGAAAAT GCAACGATAC ACATTGATGC TGTTATCGAT 
CCTTTAAGCC CTACTGGTCA AAAACTAGCA TCACTTCTTC AAGTTCTACA GAAACATGTT 
CAGACGAGCA TGAGGATTGT TCTGAATCCC ATGAGTTCCC TTGTGGACAT TCCTCTGAAA 
AATTACTACA GATATGTCTT ACCTAATACG GACGATTACA GCAGTACAGG CTTTGACGTA 
GATGGGCCTA AGGCATTTTT CGCAAATATG CCATTGTCTA AGACACTTAC CATGAATCTT 
GACGTTCCAG AGCCATGGCT GGTTGAACCA GTAATTGCCA TTCATGATCT TGATAATATA 
TTGCTTGAGA ATCTAGGAGA TACCACTACA CTGCAGGCAG TTTTTGAGGT TGAATCTCTT 
GTTCTTACAG GTCATTGCGC GGAAAAAGAT CATGAGGCTC CTCGTGGCCT TCAGCTGATT 
CTGGGAACCA AAAATAGACC GCATTTGGTT GATACCCTTG TAATGGCCAA TTTGGGTTAT 
TGGCAGATGA AAGTATCCCC AGGGGTTTGG TATTTGCAAC TTGCTCCGGG TAGAAGTTCG 
GAGCTATACG CTTTGAAAGG AGGAAATGAT GGGAGTCAAG ATCAATCCTC GCTGAAACGT 
ATAACTATCG ATGATCTGCG TGGTAAAGTT GTTCATCTAG AAGTAGTTAA GAGAAAGGGT 
AAGGAGCATG AAAAGTTGCT AGTTCCTTCA GACGGTGATG ATGCTGTGCA ACAAAACAAG 
GAAGGAAGCT GGAACTCAAA TTTCTTGAAA TGGGCGTCTG GTTTTGTTGG TGGTCGTCAA 
CAATCAATGA AGGGAGGTCC TGACAAAGAG CATGAGAAGG GTGGGCGACA AGGGAAAACG 
ATAAATATAT TCTCCATTGC TTCAGGGCAT TTATACGAGC GTTTCCTTAA GATTATGATT 
CTCAGTGTCC TGAAGAACAC CAATCGTCCT GTAAAATTCT GGTTTATTAA GAATTATCTC 
TCTCCTCAAT TTAAGGATGT GATTCCACAT ATGGCACAAG AATACAACTT TGAGTATGAG 
CTGATTACAT ACAAATGGCC GTCCTGGTTG CATAAACAGA AAGAAAAGCA ACGAATTATT 
TGGGCCTACA AAATTCTTTT CCTTGATGTC ATATTTCCTC TCTCATTGGA AAAGGTTATA 
TTTGTCGACG CAGATCAAAT TATAAGGACA GACATGGGAG AACTGTATGA TATGGATATT 
AAAGGAAGAC CTCTCGCGTA TACTCCTTTT TGTGACAACA ACAGGGAAAT GGATGGCTAT 
AAATTTTGGA AACAGGGTTT CTGGAAAGAA CATTTACGTG GTAGGCCATA CCATATCAGT 
GCTCTATACG TTGTTGACTT GGTTAAGTTC CGAGAGACAG CGGCAGGAGA TAATCTAAGA 
GTATTCTATG AGACGCTTAG CAAGGATCCA AACAGTTTGT CCAACCTAGA TCAGGATCTT 
CCTAATTATG CTCAGCATAC TGTTCCTATA TTTTCACTTC CCCAAGAGTG GTTGTGGTGT 
GAATCCTGGT GTGGAAACGC CACAAAAGCC AAGGCCAGAA CAATCGATCT TTGCAATAAT 
CCCATGACAA AGGAACCAAA GCTTCAGGGT GCCCGAAGAA TCGTAACCGA ATGGCCCGAT 
CTTGATCTCG AAGCACGAAA ATTCACAGCA AAAATCTTGG GTGAAGATGT GGAACTCAAC 
GAGCCAGTAG CTGCTCCTGC TACAGACAAG CCTAATCCTC TTCCATCAAA TGACATCTCT 
GAAGATACAG AACAAGACTT GGAATCTAAA GCAGAACTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_177278.3
CDS	35..4876
Translation	MGTTTNLRSWLYLILLFIVVVGVNAQNRRPKNVQVAVKAKWQGTPLLLEAGELISKESKQLFWEFTDAWLGSDGDDSDCKSARDCLLKISKQASTLLAQPVASLFHFSLTLRSASPRLVLYRQLADESLSSFPHGDDPSATGCCWVDTGSSLFYDVADLQSWLASAPAVGDAVQGPELFDFDHVHFDSRAGSPVAVLYGAVGTDCFRKFHLSLAKAAKEGKVTYVVRPVLPLGCEGKTRPCGAIGARDNVSLAGYGVELALKNMEYKAMDDSAIKKGITLEDPRTEDLSQDVRGFIFSKILDRKPELRSEVMAFRDYLLSSTVSDTLDVWELKDLGHQTAQRIVHASDPLQSMQEINQNFPSVVSSLSRMKLNESIKDEILSNQRMVPPGKALLALNGALLNIEDIDLYMLMDLAHQELSLANHFSKLKIPDGAIRKLLLTTPLPEPDSYRVDFRSVHVTYLNNLEEDDMYKRWRSNINEILMPAFPGQLRYIRKNLFHAVYVIDPATACGLESIETLRSLYENQLPVRFGVILYSTQLIKTIENNGGQIPSSDAVTNAQVKEDLSTMVIRLFLYIKEHHGIQTAFQFLGNLNTLRTESADSSEADIEQEHVDGAFVETILPKVKTLPQDILLKLRQEHTLKEASEASSMFVFKLGLAKLKCSFLMNGLVFDSVEEETLLNAMNEELPKIQEQVYYGQIESHTKVLDKLLSESGLSRYNPQIISGGKNKPRFVSLASSTRKGESMLNDVNYLHSPETSEDVKYVTHLLAADVATKKGMKLLHEGVRYLIGGSKSARLGVLFSSSQNADPHSLLFIKFFEKTASSFSHKEKVLYFLDKLCLFYEREYLLKTSVESASSQMFIDKVLELADEYGLSSKAYRSCLVESVDEELLKRLTKVAQFLSWELGLESDANAIISNGRVIFPVDERTFLGQDLHLLESMEFNQRVKPVQEIIEGIEWQDVDPDLLTSKYFSDVFMFVSSAMATRDRSSESARFEVLNSEYSAVLLGNENATIHIDAVIDPLSPTGQKLASLLQVLQKHVQTSMRIVLNPMSSLVDIPLKNYYRYVLPNTDDYSSTGFDVDGPKAFFANMPLSKTLTMNLDVPEPWLVEPVIAIHDLDNILLENLGDTTTLQAVFEVESLVLTGHCAEKDHEAPRGLQLILGTKNRPHLVDTLVMANLGYWQMKVSPGVWYLQLAPGRSSELYALKGGNDGSQDQSSLKRITIDDLRGKVVHLEVVKRKGKEHEKLLVPSDGDDAVQQNKEGSWNSNFLKWASGFVGGRQQSMKGGPDKEHEKGGRQGKTINIFSIASGHLYERFLKIMILSVLKNTNRPVKFWFIKNYLSPQFKDVIPHMAQEYNFEYELITYKWPSWLHKQKEKQRIIWAYKILFLDVIFPLSLEKVIFVDADQIIRTDMGELYDMDIKGRPLAYTPFCDNNREMDGYKFWKQGFWKEHLRGRPYHISALYVVDLVKFRETAAGDNLRVFYETLSKDPNSLSNLDQDLPNYAQHTVPIFSLPQEWLWCESWCGNATKAKARTIDLCNNPMTKEPKLQGARRIVTEWPDLDLEARKFTAKILGEDVELNEPVAAPATDKPNPLPSNDISEDTEQDLESKAEL

Target ORF information:

RefSeq Version	NM_105791.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 (EBS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_105791.5

ORF Insert Sequence:

ATGGGGACCA CCACCAATCT GAGATCTTGG CTGTATCTCA TTCTTCTCTT CATCGTCGTC 
GTCGGCGTCA ACGCTCAGAA TCGGAGACCT AAGAATGTTC AGGTGGCTGT TAAGGCTAAG 
TGGCAGGGTA CTCCTCTTCT CCTTGAGGCT GGTGAGTTGA TTTCCAAAGA ATCGAAGCAG 
TTGTTCTGGG AGTTTACTGA TGCTTGGCTT GGTTCCGATG GAGATGATTC CGATTGTAAA 
TCAGCTAGAG ATTGTCTTCT TAAGATATCT AAACAGGCTT CGACTCTGTT AGCTCAACCA 
GTGGCTTCTC TCTTCCATTT CTCACTTACT CTGAGATCTG CTTCGCCTAG GCTTGTGCTT 
TACCGTCAAT TGGCTGATGA GTCTCTATCT TCTTTCCCTC ATGGTGATGA TCCCTCTGCG 
ACTGGCTGCT GTTGGGTTGA TACTGGCTCC TCTCTGTTTT ACGATGTTGC AGATCTTCAA 
TCCTGGCTTG CCTCTGCTCC TGCTGTTGGA GATGCTGTTC AGGGGCCTGA GCTATTCGAC 
TTCGATCATG TTCATTTTGA TTCAAGAGCT GGAAGCCCAG TTGCTGTTCT CTATGGGGCT 
GTTGGGACTG ATTGCTTTAG GAAGTTTCAC CTGTCGCTTG CTAAAGCAGC CAAGGAGGGA 
AAAGTTACAT ATGTTGTTAG ACCAGTATTA CCTTTGGGTT GTGAAGGGAA AACTAGACCC 
TGCGGTGCCA TTGGAGCAAG GGATAATGTT AGCTTAGCTG GTTATGGTGT GGAGCTTGCT 
TTGAAGAACA TGGAGTACAA GGCTATGGAT GATAGTGCTA TAAAGAAAGG TATCACTCTT 
GAAGATCCTA GGACTGAAGA TCTTAGTCAA GATGTTAGGG GCTTTATATT CTCAAAAATC 
TTGGACAGGA AACCCGAGCT AAGATCAGAA GTCATGGCTT TTAGGGATTA TCTCCTATCA 
TCAACAGTAT CAGACACGCT TGATGTTTGG GAGCTGAAAG ATTTAGGACA CCAAACAGCG 
CAGAGAATAG TCCATGCCTC TGATCCTCTG CAGTCTATGC AAGAAATTAA TCAAAACTTT 
CCTAGTGTAG TTTCTTCATT ATCTCGCATG AAGCTCAATG AATCAATCAA AGACGAGATA 
CTTTCAAACC AGAGAATGGT TCCCCCTGGC AAGGCTTTGT TGGCTCTGAA TGGTGCCCTG 
TTGAACATCG AAGATATTGA TCTATACATG TTGATGGATT TGGCTCATCA GGAATTGTCC 
CTGGCCAACC ATTTTTCGAA GCTTAAGATA CCTGATGGAG CCATAAGGAA ACTTCTACTA 
ACTACCCCTC TCCCTGAACC GGATTCATAT CGGGTTGATT TCCGATCCGT GCATGTTACC 
TATCTCAACA ATCTGGAAGA GGATGATATG TACAAGCGTT GGCGGAGTAA TATTAACGAG 
ATTTTGATGC CTGCCTTCCC TGGACAACTA CGTTACATCC GAAAGAACCT ATTCCATGCA 
GTTTACGTCA TTGATCCAGC GACTGCCTGT GGTCTTGAGT CTATTGAAAC TCTCAGATCT 
TTATATGAAA ATCAACTCCC TGTGAGATTT GGGGTGATAT TATATTCAAC TCAACTAATC 
AAGACAATCG AGAATAATGG TGGTCAAATC CCATCTTCTG ATGCAGTGAC TAATGCCCAA 
GTTAAAGAGG ACTTATCGAC TATGGTCATA CGTCTTTTTC TGTACATCAA GGAGCACCAT 
GGGATTCAAA CAGCTTTTCA GTTCTTGGGC AATTTAAACA CACTAAGGAC TGAATCTGCT 
GATTCTTCCG AAGCAGACAT TGAACAGGAG CACGTTGATG GTGCATTTGT TGAGACAATT 
TTACCAAAGG TTAAAACACT CCCTCAGGAT ATATTGCTGA AACTGCGACA AGAGCACACT 
TTGAAGGAAG CATCTGAAGC AAGCTCCATG TTTGTATTTA AGTTGGGTCT GGCGAAATTG 
AAGTGTTCCT TTTTGATGAA TGGCCTTGTC TTTGATTCTG TTGAGGAAGA AACTCTTTTA 
AATGCCATGA ATGAAGAGCT TCCCAAGATA CAAGAACAAG TTTATTACGG CCAGATAGAA 
TCCCATACTA AGGTTTTGGA CAAGTTGCTT TCGGAAAGTG GTCTTAGCCG TTATAATCCA 
CAGATTATTA GTGGAGGAAA GAACAAACCA AGGTTTGTGT CGCTAGCTTC ATCTACCAGG 
AAAGGGGAAT CTATGTTGAA TGATGTTAAC TACTTGCATT CTCCTGAAAC TTCAGAGGAT 
GTGAAGTATG TGACTCATCT CCTTGCTGCT GATGTTGCAA CGAAGAAAGG AATGAAATTG 
TTACATGAAG GAGTCCGTTA CCTGATTGGA GGTTCCAAAA GTGCTCGTCT TGGAGTCTTA 
TTTAGTAGCA GCCAGAATGC TGACCCACAT AGCCTTCTAT TTATAAAGTT TTTCGAAAAA 
ACTGCTTCAT CGTTTAGTCA TAAGGAAAAG GTGCTATACT TCTTGGATAA ACTATGCTTG 
TTTTACGAGC GTGAATATCT GTTAAAAACA TCAGTGGAAT CAGCTAGCTC TCAAATGTTT 
ATTGATAAAG TCCTTGAGCT TGCTGATGAG TATGGGCTAT CTTCTAAGGC ATACCGATCT 
TGTCTCGTTG AATCAGTGGA TGAAGAATTA CTTAAGCGTT TGACAAAGGT CGCCCAATTC 
TTGTCTTGGG AACTTGGACT TGAATCTGAT GCAAATGCAA TAATTTCAAA TGGAAGGGTG 
ATATTTCCTG TGGATGAGAG GACATTTTTG GGCCAAGATT TACATCTTCT TGAGTCAATG 
GAGTTCAATC AAAGAGTAAA GCCTGTTCAA GAAATTATTG AAGGCATTGA ATGGCAAGAT 
GTGGATCCCG ATCTGCTGAC AAGTAAATAT TTTAGCGATG TCTTCATGTT TGTGTCATCT 
GCAATGGCAA CACGGGATCG TAGTTCTGAA AGTGCGCGGT TTGAGGTGTT GAATTCAGAA 
TACAGTGCGG TTTTGCTGGG AAATGAAAAT GCAACGATAC ACATTGATGC TGTTATCGAT 
CCTTTAAGCC CTACTGGTCA AAAACTAGCA TCACTTCTTC AAGTTCTACA GAAACATGTT 
CAGACGAGCA TGAGGATTGT TCTGAATCCC ATGAGTTCCC TTGTGGACAT TCCTCTGAAA 
AATTACTACA GATATGTCTT ACCTAATACG GACGATTACA GCAGTACAGG CTTTGACGTA 
GATGGGCCTA AGGCATTTTT CGCAAATATG CCATTGTCTA AGACACTTAC CATGAATCTT 
GACGTTCCAG AGCCATGGCT GGTTGAACCA GTAATTGCCA TTCATGATCT TGATAATATA 
TTGCTTGAGA ATCTAGGAGA TACCACTACA CTGCAGGCAG TTTTTGAGGT TGAATCTCTT 
GTTCTTACAG GTCATTGCGC GGAAAAAGAT CATGAGGCTC CTCGTGGCCT TCAGCTGATT 
CTGGGAACCA AAAATAGACC GCATTTGGTT GATACCCTTG TAATGGCCAA TTTGGGTTAT 
TGGCAGATGA AAGTATCCCC AGGGGTTTGG TATTTGCAAC TTGCTCCGGG TAGAAGTTCG 
GAGCTATACG CTTTGAAAGG AGGAAATGAT GGGAGTCAAG ATCAATCCTC GCTGAAACGT 
ATAACTATCG ATGATCTGCG TGGTAAAGTT GTTCATCTAG AAGTAGTTAA GAGAAAGGGT 
AAGGAGCATG AAAAGTTGCT AGTTCCTTCA GACGGTGATG ATGCTGTGCA ACAAAACAAG 
GAAGGAAGCT GGAACTCAAA TTTCTTGAAA TGGGCGTCTG GTTTTGTTGG TGGTCGTCAA 
CAATCAATGA AGGGAGGTCC TGACAAAGAG CATGAGAAGG GTGGGCGACA AGGGAAAACG 
ATAAATATAT TCTCCATTGC TTCAGGGCAT TTATACGAGC GTTTCCTTAA GATTATGATT 
CTCAGTGTCC TGAAGAACAC CAATCGTCCT GTAAAATTCT GGTTTATTAA GAATTATCTC 
TCTCCTCAAT TTAAGGATGT GATTCCACAT ATGGCACAAG AATACAACTT TGAGTATGAG 
CTGATTACAT ACAAATGGCC GTCCTGGTTG CATAAACAGA AAGAAAAGCA ACGAATTATT 
TGGGCCTACA AAATTCTTTT CCTTGATGTC ATATTTCCTC TCTCATTGGA AAAGGTTATA 
TTTGTCGACG CAGATCAAAT TATAAGGACA GACATGGGAG AACTGTATGA TATGGATATT 
AAAGGAAGAC CTCTCGCGTA TACTCCTTTT TGTGACAACA ACAGGGAAAT GGATGGCTAT 
AAATTTTGGA AACAGGGTTT CTGGAAAGAA CATTTACGTG GTAGGCCATA CCATATCAGT 
GCTCTATACG TTGTTGACTT GGTTAAGTTC CGAGAGACAG CGGCAGGAGA TAATCTAAGA 
GTATTCTATG AGACGCTTAG CAAGGATCCA AACAGTTTGT CCAACCTAGA TCAGGATCTT 
CCTAATTATG CTCAGCATAC TGTTCCTATA TTTTCACTTC CCCAAGAGTG GTTGTGGTGT 
GAATCCTGGT GTGGAAACGC CACAAAAGCC AAGGCCAGAA CAATCGATCT TTGCAATAAT 
CCCATGACAA AGGAACCAAA GCTTCAGGGT GCCCGAAGAA TCGTAACCGA ATGGCCCGAT 
CTTGATCTCG AAGCACGAAA ATTCACAGCA AAAATCTTGG GTGAAGATGT GGAACTCAAC 
GAGCCAGTAG CTGCTCCTGC TACAGACAAG CCTAATCCTC TTCCATCAAA TGACATCTCT 
GAAGATACAG AACAAGACTT GGAATCTAAA GCAGAACTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EBS1 ( NM_001198441.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185370.1 Arabidopsis thaliana EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 (EBS1), mRNA.

Price & Availability↑

CloneID	OAb29487
Clone ID Related Accession (Same CDS sequence)	NM_001198441.1
Accession Version	NM_001198441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	EMS-MUTAGENIZED BRI1 SUPPRESSOR 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGGGACCA CCACCAATCT GAGATCTTGG CTGTATCTCA TTCTTCTCTT CATCGTCGTC 
GTCGGCGTCA ACGCTCAGAA TCGGAGACCT AAGAATGTTC AGGTGGCTGT TAAGGCTAAG 
TGGCAGGGTA CTCCTCTTCT CCTTGAGGCT GGTGAGTTGA TTTCCAAAGA ATCGAAGCAG 
TTGTTCTGGG AGTTTACTGA TGCTTGGCTT GGTTCCGATG GAGATGATTC CGATTGTAAA 
TCAGCTAGAG ATTGTCTTCT TAAGATATCT AAACAGGCTT CGACTCTGTT AGCTCAACCA 
GTGGCTTCTC TCTTCCATTT CTCACTTACT CTGAGATCTG CTTCGCCTAG GCTTGTGCTT 
TACCGTCAAT TGGCTGATGA GTCTCTATCT TCTTTCCCTC ATGGTGATGA TCCCTCTGCG 
ACTGGCTGCT GTTGGGTTGA TACTGGCTCC TCTCTGTTTT ACGATGTTGC AGATCTTCAA 
TCCTGGCTTG CCTCTGCTCC TGCTGTTGGA GATGCTGTTC AGGGGCCTGA GCTATTCGAC 
TTCGATCATG TTCATTTTGA TTCAAGAGCT GGAAGCCCAG TTGCTGTTCT CTATGGGGCT 
GTTGGGACTG ATTGCTTTAG GAAGTTTCAC CTGTCGCTTG CTAAAGCAGC CAAGGAGGGA 
AAAGTTACAT ATGTTGTTAG ACCAGTATTA CCTTTGGGTT GTGAAGGGAA AACTAGACCC 
TGCGGTGCCA TTGGAGCAAG GGATAATGTT AGCTTAGCTG GTTATGGTGT GGAGCTTGCT 
TTGAAGAACA TGGAGTACAA GGCTATGGAT GATAGTGCTA TAAAGAAAGG TATCACTCTT 
GAAGATCCTA GGACTGAAGA TCTTAGTCAA GATGTTAGGG GCTTTATATT CTCAAAAATC 
TTGGACAGGA AACCCGAGCT AAGATCAGAA GTCATGGCTT TTAGGGATTA TCTCCTATCA 
TCAACAGTAT CAGACACGCT TGATGTTTGG GAGCTGAAAG ATTTAGGACA CCAAACAGCG 
CAGAGAATAG TCCATGCCTC TGATCCTCTG CAGTCTATGC AAGAAATTAA TCAAAACTTT 
CCTAGTGTAG TTTCTTCATT ATCTCGCATG AAGCTCAATG AATCAATCAA AGACGAGATA 
CTTTCAAACC AGAGAATGGT TCCCCCTGGC AAGGCTTTGT TGGCTCTGAA TGGTGCCCTG 
TTGAACATCG AAGATATTGA TCTATACATG TTGATGGATT TGGCTCATCA GGAATTGTCC 
CTGGCCAACC ATTTTTCGAA GCTTAAGATA CCTGATGGAG CCATAAGGAA ACTTCTACTA 
ACTACCCCTC TCCCTGAACC GGATTCATAT CGGGTTGATT TCCGATCCGT GCATGTTACC 
TATCTCAACA ATCTGGAAGA GGATGATATG TACAAGCGTT GGCGGAGTAA TATTAACGAG 
ATTTTGATGC CTGCCTTCCC TGGACAACTA CGTTACATCC GAAAGAACCT ATTCCATGCA 
GTTTACGTCA TTGATCCAGC GACTGCCTGT GGTCTTGAGT CTATTGAAAC TCTCAGATCT 
TTATATGAAA ATCAACTCCC TGTGAGATTT GGGGTGATAT TATATTCAAC TCAACTAATC 
AAGACAATCG AGAATAATGG TGGTCAAATC CCATCTTCTG ATGCAGTGAC TAATGCCCAA 
GTTAAAGAGG ACTTATCGAC TATGGTCATA CGTCTTTTTC TGTACATCAA GGAGCACCAT 
GGGATTCAAA CAGCTTTTCA GTTCTTGGGC AATTTAAACA CACTAAGGAC TGAATCTGCT 
GATTCTTCCG AAGCAGACAT TGAACAGGAG CACGTTGATG GTGCATTTGT TGAGACAATT 
TTACCAAAGG TTAAAACACT CCCTCAGGAT ATATTGCTGA AACTGCGACA AGAGCACACT 
TTGAAGGAAG CATCTGAAGC AAGCTCCATG TTTGTATTTA AGTTGGGTCT GGCGAAATTG 
AAGTGTTCCT TTTTGATGAA TGGCCTTGTC TTTGATTCTG TTGAGGAAGA AACTCTTTTA 
AATGCCATGA ATGAAGAGCT TCCCAAGATA CAAGAACAAG TTTATTACGG CCAGATAGAA 
TCCCATACTA AGGTTTTGGA CAAGTTGCTT TCGGAAAGTG GTCTTAGCCG TTATAATCCA 
CAGATTATTA GTGGAGGAAA GAACAAACCA AGGTTTGTGT CGCTAGCTTC ATCTACCAGG 
AAAGGGGAAT CTATGTTGAA TGATGTTAAC TACTTGCATT CTCCTGAAAC TTCAGAGGAT 
GTGAAGTATG TGACTCATCT CCTTGCTGCT GATGTTGCAA CGAAGAAAGG AATGAAATTG 
TTACATGAAG GAGTCCGTTA CCTGATTGGA GGTTCCAAAA GTGCTCGTCT TGGAGTCTTA 
TTTAGTAGCA GCCAGAATGC TGACCCACAT AGCCTTCTAT TTATAAAGTT TTTCGAAAAA 
ACTGCTTCAT CGTTTAGTCA TAAGGAAAAG GTGCTATACT TCTTGGATAA ACTATGCTTG 
TTTTACGAGC GTGAATATCT GTTAAAAACA TCAGTGGAAT CAGCTAGCTC TCAAATGTTT 
ATTGATAAAG TCCTTGAGCT TGCTGATGAG TATGGGCTAT CTTCTAAGGC ATACCGATCT 
TGTCTCGTTG AATCAGTGGA TGAAGAATTA CTTAAGCGTT TGACAAAGGT CGCCCAATTC 
TTGTCTTGGG AACTTGGACT TGAATCTGAT GCAAATGCAA TAATTTCAAA TGGAAGGGTG 
ATATTTCCTG TGGATGAGAG GACATTTTTG GGCCAAGATT TACATCTTCT TGAGTCAATG 
GAGTTCAATC AAAGAGTAAA GCCTGTTCAA GAAATTATTG AAGGCATTGA ATGGCAAGAT 
GTGGATCCCG ATCTGCTGAC AAGTAAATAT TTTAGCGATG TCTTCATGTT TGTGTCATCT 
GCAATGGCAA CACGGGATCG TAGTTCTGAA AGTGCGCGGT TTGAGGTGTT GAATTCAGAA 
TACAGTGCGG TTTTGCTGGG AAATGAAAAT GCAACGATAC ACATTGATGC TGTTATCGAT 
CCTTTAAGCC CTACTGGTCA AAAACTAGCA TCACTTCTTC AAGTTCTACA GAAACATGTT 
CAGACGAGCA TGAGGATTGT TCTGAATCCC ATGAGTTCCC TTGTGGACAT TCCTCTGAAA 
AATTACTACA GATATGTCTT ACCTAATACG GACGATTACA GCAGTACAGG CTTTGACGTA 
GATGGGCCTA AGGCATTTTT CGCAAATATG CCATTGTCTA AGACACTTAC CATGAATCTT 
GACGTTCCAG AGCCATGGCT GGTTGAACCA GTAATTGCCA TTCATGATCT TGATAATATA 
TTGCTTGAGA ATCTAGGAGA TACCACTACA CTGCAGGCAG TTTTTGAGGT TGAATCTCTT 
GTTCTTACAG GTCATTGCGC GGAAAAAGAT CATGAGGCTC CTCGTGGCCT TCAGCTGATT 
CTGGGAACCA AAAATAGACC GCATTTGGTT GATACCCTTG TAATGGCCAA TTTGGGTTAT 
TGGCAGATGA AAGTATCCCC AGGGGTTTGG TATTTGCAAC TTGCTCCGGG TAGAAGTTCG 
GAGCTATACG CTTTGAAAGG AGGAAATGAT GGGAGTCAAG ATCAATCCTC GCTGAAACGT 
ATAACTATCG ATGATCTGCG TGGTAAAGTT GTTCATCTAG AAGTAGTTAA GAGAAAGGGT 
AAGGAGCATG AAAAGTTGCT AGTTCCTTCA GACGGTGATG ATGCTGTGCA ACAAAACAAG 
GAACAGGGAA GCTGGAACTC AAATTTCTTG AAATGGGCGT CTGGTTTTGT TGGTGGTCGT 
CAACAATCAA TGAAGGGAGG TCCTGACAAA GAGCATGAGA AGGGTGGGCG ACAAGGGAAA 
ACGATAAATA TATTCTCCAT TGCTTCAGGG CATTTATACG AGCGTTTCCT TAAGATTATG 
ATTCTCAGTG TCCTGAAGAA CACCAATCGT CCTGTAAAAT TCTGGTTTAT TAAGAATTAT 
CTCTCTCCTC AATTTAAGGA TGTGATTCCA CATATGGCAC AAGAATACAA CTTTGAGTAT 
GAGCTGATTA CATACAAATG GCCGTCCTGG TTGCATAAAC AGAAAGAAAA GCAACGAATT 
ATTTGGGCCT ACAAAATTCT TTTCCTTGAT GTCATATTTC CTCTCTCATT GGAAAAGGTT 
ATATTTGTCG ACGCAGATCA AATTATAAGG ACAGACATGG GAGAACTGTA TGATATGGAT 
ATTAAAGGAA GACCTCTCGC GTATACTCCT TTTTGTGACA ACAACAGGGA AATGGATGGC 
TATAAATTTT GGAAACAGGG TTTCTGGAAA GAACATTTAC GTGGTAGGCC ATACCATATC 
AGTGCTCTAT ACGTTGTTGA CTTGGTTAAG TTCCGAGAGA CAGCGGCAGG AGATAATCTA 
AGAGTATTCT ATGAGACGCT TAGCAAGGAT CCAAACAGTT TGTCCAACCT AGATCAGGAT 
CTTCCTAATT ATGCTCAGCA TACTGTTCCT ATATTTTCAC TTCCCCAAGA GTGGTTGTGG 
TGTGAATCCT GGTGTGGAAA CGCCACAAAA GCCAAGGCCA GAACAATCGA TCTTTGCAAT 
AATCCCATGA CAAAGGAACC AAAGCTTCAG GGTGCCCGAA GAATCGTAAC CGAATGGCCC 
GATCTTGATC TCGAAGCACG AAAATTCACA GCAAAAATCT TGGGTGAAGA TGTGGAACTC 
AACGAGCCAG TAGCTGCTCC TGCTACAGAC AAGCCTAATC CTCTTCCATC AAATGACATC 
TCTGAAGATA CAGAACAAGA CTTGGAATCT AAAGCAGAAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185370.1
CDS	35..4879
Translation	MGTTTNLRSWLYLILLFIVVVGVNAQNRRPKNVQVAVKAKWQGTPLLLEAGELISKESKQLFWEFTDAWLGSDGDDSDCKSARDCLLKISKQASTLLAQPVASLFHFSLTLRSASPRLVLYRQLADESLSSFPHGDDPSATGCCWVDTGSSLFYDVADLQSWLASAPAVGDAVQGPELFDFDHVHFDSRAGSPVAVLYGAVGTDCFRKFHLSLAKAAKEGKVTYVVRPVLPLGCEGKTRPCGAIGARDNVSLAGYGVELALKNMEYKAMDDSAIKKGITLEDPRTEDLSQDVRGFIFSKILDRKPELRSEVMAFRDYLLSSTVSDTLDVWELKDLGHQTAQRIVHASDPLQSMQEINQNFPSVVSSLSRMKLNESIKDEILSNQRMVPPGKALLALNGALLNIEDIDLYMLMDLAHQELSLANHFSKLKIPDGAIRKLLLTTPLPEPDSYRVDFRSVHVTYLNNLEEDDMYKRWRSNINEILMPAFPGQLRYIRKNLFHAVYVIDPATACGLESIETLRSLYENQLPVRFGVILYSTQLIKTIENNGGQIPSSDAVTNAQVKEDLSTMVIRLFLYIKEHHGIQTAFQFLGNLNTLRTESADSSEADIEQEHVDGAFVETILPKVKTLPQDILLKLRQEHTLKEASEASSMFVFKLGLAKLKCSFLMNGLVFDSVEEETLLNAMNEELPKIQEQVYYGQIESHTKVLDKLLSESGLSRYNPQIISGGKNKPRFVSLASSTRKGESMLNDVNYLHSPETSEDVKYVTHLLAADVATKKGMKLLHEGVRYLIGGSKSARLGVLFSSSQNADPHSLLFIKFFEKTASSFSHKEKVLYFLDKLCLFYEREYLLKTSVESASSQMFIDKVLELADEYGLSSKAYRSCLVESVDEELLKRLTKVAQFLSWELGLESDANAIISNGRVIFPVDERTFLGQDLHLLESMEFNQRVKPVQEIIEGIEWQDVDPDLLTSKYFSDVFMFVSSAMATRDRSSESARFEVLNSEYSAVLLGNENATIHIDAVIDPLSPTGQKLASLLQVLQKHVQTSMRIVLNPMSSLVDIPLKNYYRYVLPNTDDYSSTGFDVDGPKAFFANMPLSKTLTMNLDVPEPWLVEPVIAIHDLDNILLENLGDTTTLQAVFEVESLVLTGHCAEKDHEAPRGLQLILGTKNRPHLVDTLVMANLGYWQMKVSPGVWYLQLAPGRSSELYALKGGNDGSQDQSSLKRITIDDLRGKVVHLEVVKRKGKEHEKLLVPSDGDDAVQQNKEQGSWNSNFLKWASGFVGGRQQSMKGGPDKEHEKGGRQGKTINIFSIASGHLYERFLKIMILSVLKNTNRPVKFWFIKNYLSPQFKDVIPHMAQEYNFEYELITYKWPSWLHKQKEKQRIIWAYKILFLDVIFPLSLEKVIFVDADQIIRTDMGELYDMDIKGRPLAYTPFCDNNREMDGYKFWKQGFWKEHLRGRPYHISALYVVDLVKFRETAAGDNLRVFYETLSKDPNSLSNLDQDLPNYAQHTVPIFSLPQEWLWCESWCGNATKAKARTIDLCNNPMTKEPKLQGARRIVTEWPDLDLEARKFTAKILGEDVELNEPVAAPATDKPNPLPSNDISEDTEQDLESKAEL

Target ORF information:

RefSeq Version	NM_001198441.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana EMS-MUTAGENIZED BRI1 SUPPRESSOR 1 (EBS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198441.1

ORF Insert Sequence:

ATGGGGACCA CCACCAATCT GAGATCTTGG CTGTATCTCA TTCTTCTCTT CATCGTCGTC 
GTCGGCGTCA ACGCTCAGAA TCGGAGACCT AAGAATGTTC AGGTGGCTGT TAAGGCTAAG 
TGGCAGGGTA CTCCTCTTCT CCTTGAGGCT GGTGAGTTGA TTTCCAAAGA ATCGAAGCAG 
TTGTTCTGGG AGTTTACTGA TGCTTGGCTT GGTTCCGATG GAGATGATTC CGATTGTAAA 
TCAGCTAGAG ATTGTCTTCT TAAGATATCT AAACAGGCTT CGACTCTGTT AGCTCAACCA 
GTGGCTTCTC TCTTCCATTT CTCACTTACT CTGAGATCTG CTTCGCCTAG GCTTGTGCTT 
TACCGTCAAT TGGCTGATGA GTCTCTATCT TCTTTCCCTC ATGGTGATGA TCCCTCTGCG 
ACTGGCTGCT GTTGGGTTGA TACTGGCTCC TCTCTGTTTT ACGATGTTGC AGATCTTCAA 
TCCTGGCTTG CCTCTGCTCC TGCTGTTGGA GATGCTGTTC AGGGGCCTGA GCTATTCGAC 
TTCGATCATG TTCATTTTGA TTCAAGAGCT GGAAGCCCAG TTGCTGTTCT CTATGGGGCT 
GTTGGGACTG ATTGCTTTAG GAAGTTTCAC CTGTCGCTTG CTAAAGCAGC CAAGGAGGGA 
AAAGTTACAT ATGTTGTTAG ACCAGTATTA CCTTTGGGTT GTGAAGGGAA AACTAGACCC 
TGCGGTGCCA TTGGAGCAAG GGATAATGTT AGCTTAGCTG GTTATGGTGT GGAGCTTGCT 
TTGAAGAACA TGGAGTACAA GGCTATGGAT GATAGTGCTA TAAAGAAAGG TATCACTCTT 
GAAGATCCTA GGACTGAAGA TCTTAGTCAA GATGTTAGGG GCTTTATATT CTCAAAAATC 
TTGGACAGGA AACCCGAGCT AAGATCAGAA GTCATGGCTT TTAGGGATTA TCTCCTATCA 
TCAACAGTAT CAGACACGCT TGATGTTTGG GAGCTGAAAG ATTTAGGACA CCAAACAGCG 
CAGAGAATAG TCCATGCCTC TGATCCTCTG CAGTCTATGC AAGAAATTAA TCAAAACTTT 
CCTAGTGTAG TTTCTTCATT ATCTCGCATG AAGCTCAATG AATCAATCAA AGACGAGATA 
CTTTCAAACC AGAGAATGGT TCCCCCTGGC AAGGCTTTGT TGGCTCTGAA TGGTGCCCTG 
TTGAACATCG AAGATATTGA TCTATACATG TTGATGGATT TGGCTCATCA GGAATTGTCC 
CTGGCCAACC ATTTTTCGAA GCTTAAGATA CCTGATGGAG CCATAAGGAA ACTTCTACTA 
ACTACCCCTC TCCCTGAACC GGATTCATAT CGGGTTGATT TCCGATCCGT GCATGTTACC 
TATCTCAACA ATCTGGAAGA GGATGATATG TACAAGCGTT GGCGGAGTAA TATTAACGAG 
ATTTTGATGC CTGCCTTCCC TGGACAACTA CGTTACATCC GAAAGAACCT ATTCCATGCA 
GTTTACGTCA TTGATCCAGC GACTGCCTGT GGTCTTGAGT CTATTGAAAC TCTCAGATCT 
TTATATGAAA ATCAACTCCC TGTGAGATTT GGGGTGATAT TATATTCAAC TCAACTAATC 
AAGACAATCG AGAATAATGG TGGTCAAATC CCATCTTCTG ATGCAGTGAC TAATGCCCAA 
GTTAAAGAGG ACTTATCGAC TATGGTCATA CGTCTTTTTC TGTACATCAA GGAGCACCAT 
GGGATTCAAA CAGCTTTTCA GTTCTTGGGC AATTTAAACA CACTAAGGAC TGAATCTGCT 
GATTCTTCCG AAGCAGACAT TGAACAGGAG CACGTTGATG GTGCATTTGT TGAGACAATT 
TTACCAAAGG TTAAAACACT CCCTCAGGAT ATATTGCTGA AACTGCGACA AGAGCACACT 
TTGAAGGAAG CATCTGAAGC AAGCTCCATG TTTGTATTTA AGTTGGGTCT GGCGAAATTG 
AAGTGTTCCT TTTTGATGAA TGGCCTTGTC TTTGATTCTG TTGAGGAAGA AACTCTTTTA 
AATGCCATGA ATGAAGAGCT TCCCAAGATA CAAGAACAAG TTTATTACGG CCAGATAGAA 
TCCCATACTA AGGTTTTGGA CAAGTTGCTT TCGGAAAGTG GTCTTAGCCG TTATAATCCA 
CAGATTATTA GTGGAGGAAA GAACAAACCA AGGTTTGTGT CGCTAGCTTC ATCTACCAGG 
AAAGGGGAAT CTATGTTGAA TGATGTTAAC TACTTGCATT CTCCTGAAAC TTCAGAGGAT 
GTGAAGTATG TGACTCATCT CCTTGCTGCT GATGTTGCAA CGAAGAAAGG AATGAAATTG 
TTACATGAAG GAGTCCGTTA CCTGATTGGA GGTTCCAAAA GTGCTCGTCT TGGAGTCTTA 
TTTAGTAGCA GCCAGAATGC TGACCCACAT AGCCTTCTAT TTATAAAGTT TTTCGAAAAA 
ACTGCTTCAT CGTTTAGTCA TAAGGAAAAG GTGCTATACT TCTTGGATAA ACTATGCTTG 
TTTTACGAGC GTGAATATCT GTTAAAAACA TCAGTGGAAT CAGCTAGCTC TCAAATGTTT 
ATTGATAAAG TCCTTGAGCT TGCTGATGAG TATGGGCTAT CTTCTAAGGC ATACCGATCT 
TGTCTCGTTG AATCAGTGGA TGAAGAATTA CTTAAGCGTT TGACAAAGGT CGCCCAATTC 
TTGTCTTGGG AACTTGGACT TGAATCTGAT GCAAATGCAA TAATTTCAAA TGGAAGGGTG 
ATATTTCCTG TGGATGAGAG GACATTTTTG GGCCAAGATT TACATCTTCT TGAGTCAATG 
GAGTTCAATC AAAGAGTAAA GCCTGTTCAA GAAATTATTG AAGGCATTGA ATGGCAAGAT 
GTGGATCCCG ATCTGCTGAC AAGTAAATAT TTTAGCGATG TCTTCATGTT TGTGTCATCT 
GCAATGGCAA CACGGGATCG TAGTTCTGAA AGTGCGCGGT TTGAGGTGTT GAATTCAGAA 
TACAGTGCGG TTTTGCTGGG AAATGAAAAT GCAACGATAC ACATTGATGC TGTTATCGAT 
CCTTTAAGCC CTACTGGTCA AAAACTAGCA TCACTTCTTC AAGTTCTACA GAAACATGTT 
CAGACGAGCA TGAGGATTGT TCTGAATCCC ATGAGTTCCC TTGTGGACAT TCCTCTGAAA 
AATTACTACA GATATGTCTT ACCTAATACG GACGATTACA GCAGTACAGG CTTTGACGTA 
GATGGGCCTA AGGCATTTTT CGCAAATATG CCATTGTCTA AGACACTTAC CATGAATCTT 
GACGTTCCAG AGCCATGGCT GGTTGAACCA GTAATTGCCA TTCATGATCT TGATAATATA 
TTGCTTGAGA ATCTAGGAGA TACCACTACA CTGCAGGCAG TTTTTGAGGT TGAATCTCTT 
GTTCTTACAG GTCATTGCGC GGAAAAAGAT CATGAGGCTC CTCGTGGCCT TCAGCTGATT 
CTGGGAACCA AAAATAGACC GCATTTGGTT GATACCCTTG TAATGGCCAA TTTGGGTTAT 
TGGCAGATGA AAGTATCCCC AGGGGTTTGG TATTTGCAAC TTGCTCCGGG TAGAAGTTCG 
GAGCTATACG CTTTGAAAGG AGGAAATGAT GGGAGTCAAG ATCAATCCTC GCTGAAACGT 
ATAACTATCG ATGATCTGCG TGGTAAAGTT GTTCATCTAG AAGTAGTTAA GAGAAAGGGT 
AAGGAGCATG AAAAGTTGCT AGTTCCTTCA GACGGTGATG ATGCTGTGCA ACAAAACAAG 
GAACAGGGAA GCTGGAACTC AAATTTCTTG AAATGGGCGT CTGGTTTTGT TGGTGGTCGT 
CAACAATCAA TGAAGGGAGG TCCTGACAAA GAGCATGAGA AGGGTGGGCG ACAAGGGAAA 
ACGATAAATA TATTCTCCAT TGCTTCAGGG CATTTATACG AGCGTTTCCT TAAGATTATG 
ATTCTCAGTG TCCTGAAGAA CACCAATCGT CCTGTAAAAT TCTGGTTTAT TAAGAATTAT 
CTCTCTCCTC AATTTAAGGA TGTGATTCCA CATATGGCAC AAGAATACAA CTTTGAGTAT 
GAGCTGATTA CATACAAATG GCCGTCCTGG TTGCATAAAC AGAAAGAAAA GCAACGAATT 
ATTTGGGCCT ACAAAATTCT TTTCCTTGAT GTCATATTTC CTCTCTCATT GGAAAAGGTT 
ATATTTGTCG ACGCAGATCA AATTATAAGG ACAGACATGG GAGAACTGTA TGATATGGAT 
ATTAAAGGAA GACCTCTCGC GTATACTCCT TTTTGTGACA ACAACAGGGA AATGGATGGC 
TATAAATTTT GGAAACAGGG TTTCTGGAAA GAACATTTAC GTGGTAGGCC ATACCATATC 
AGTGCTCTAT ACGTTGTTGA CTTGGTTAAG TTCCGAGAGA CAGCGGCAGG AGATAATCTA 
AGAGTATTCT ATGAGACGCT TAGCAAGGAT CCAAACAGTT TGTCCAACCT AGATCAGGAT 
CTTCCTAATT ATGCTCAGCA TACTGTTCCT ATATTTTCAC TTCCCCAAGA GTGGTTGTGG 
TGTGAATCCT GGTGTGGAAA CGCCACAAAA GCCAAGGCCA GAACAATCGA TCTTTGCAAT 
AATCCCATGA CAAAGGAACC AAAGCTTCAG GGTGCCCGAA GAATCGTAAC CGAATGGCCC 
GATCTTGATC TCGAAGCACG AAAATTCACA GCAAAAATCT TGGGTGAAGA TGTGGAACTC 
AACGAGCCAG TAGCTGCTCC TGCTACAGAC AAGCCTAATC CTCTTCCATC AAATGACATC 
TCTGAAGATA CAGAACAAGA CTTGGAATCT AAAGCAGAAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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EBS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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