AT1G57610 cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following AT1G57610 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT1G57610 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb04072 NM_001084264.2
Latest version!
Arabidopsis thaliana calcium uniporter (DUF607) (AT1G57610), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OAb04072 NM_001333801.1
Latest version!
Arabidopsis thaliana calcium uniporter (DUF607) (AT1G57610), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OAb04073 NM_202314.3
Latest version!
Arabidopsis thaliana calcium uniporter (DUF607) (AT1G57610), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OAb04073 NM_104558.4
Latest version!
Arabidopsis thaliana calcium uniporter (DUF607) (AT1G57610), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb04072
    Clone ID Related Accession (Same CDS sequence) NM_001333801.1 , NM_001084264.2
    Accession Version NM_001333801.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product calcium uniporter (DUF607)
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTGGTCGG TGATGGGTTT AGTGAGACGT ACGGCTATGT CCTCCACCGT GAATAAAGCT 
    TCTCCGGTTA GGTCTTTGCT TGGTGGGTTT AGATGTCTCA ACGTTGAGTC TAAGGAAGAA
    GATGAGAAAA AGGATATGAC TGTGTTAGAA GCTAAGAAGT TAATGAGATT AGTGAATGTT
    GAAGATATGA AGAAGAAGCT TATTGGTATG GGTGATAAAG AAATGGTTAC TTACACTACA
    CTTATTGAAG CATCTCAAGG TTTAGGTATC GCTAGATCTC TTGATGAAGC TCATGCTTTT
    GCTCGTGTTC TTGATGATGC TGGTGTTATT TTGATCTTTC GTGATAAAGT CTATCTTCAT
    CCTCATAAGG TGGTGGATTT GATTAGAAAG GCAGTGCCTT TAGGTTTGAA TCCAGATGAT
    GAGTTGATTA GAGAAGAGTT TGATAAGATG AGAAGTATGA AGGAAGAGAT TGATGTGTTA
    GCTCATCAAC AAGTGAGGAA GATTCTATGG GGTGGTCTTG GTTACTCAGT GGTTCAAATT
    GGAATCTTTG TTAGGCTAAC TTTTTGGGAG TTTTCTTGGG ATGTGATGGA ACCAATAACG
    TTTTTCACTA CAGCTACTGG GATTATTGCA GAGGAAGTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001322074.1
    CDS460..1104
    Translation

    Target ORF information:

    RefSeq Version NM_001333801.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana calcium uniporter (DUF607) (AT1G57610), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001333801.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTGGTCGG TGATGGGTTT AGTGAGACGT ACGGCTATGT CCTCCACCGT GAATAAAGCT 
    TCTCCGGTTA GGTCTTTGCT TGGTGGGTTT AGATGTCTCA ACGTTGAGTC TAAGGAAGAA
    GATGAGAAAA AGGATATGAC TGTGTTAGAA GCTAAGAAGT TAATGAGATT AGTGAATGTT
    GAAGATATGA AGAAGAAGCT TATTGGTATG GGTGATAAAG AAATGGTTAC TTACACTACA
    CTTATTGAAG CATCTCAAGG TTTAGGTATC GCTAGATCTC TTGATGAAGC TCATGCTTTT
    GCTCGTGTTC TTGATGATGC TGGTGTTATT TTGATCTTTC GTGATAAAGT CTATCTTCAT
    CCTCATAAGG TGGTGGATTT GATTAGAAAG GCAGTGCCTT TAGGTTTGAA TCCAGATGAT
    GAGTTGATTA GAGAAGAGTT TGATAAGATG AGAAGTATGA AGGAAGAGAT TGATGTGTTA
    GCTCATCAAC AAGTGAGGAA GATTCTATGG GGTGGTCTTG GTTACTCAGT GGTTCAAATT
    GGAATCTTTG TTAGGCTAAC TTTTTGGGAG TTTTCTTGGG ATGTGATGGA ACCAATAACG
    TTTTTCACTA CAGCTACTGG GATTATTGCA GAGGAAGTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb04072
    Clone ID Related Accession (Same CDS sequence) NM_001333801.1 , NM_001084264.2
    Accession Version NM_001084264.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product calcium uniporter (DUF607)
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Apr 24, 2008 this sequence version replaced NM_001084264.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTGGTCGG TGATGGGTTT AGTGAGACGT ACGGCTATGT CCTCCACCGT GAATAAAGCT 
    TCTCCGGTTA GGTCTTTGCT TGGTGGGTTT AGATGTCTCA ACGTTGAGTC TAAGGAAGAA
    GATGAGAAAA AGGATATGAC TGTGTTAGAA GCTAAGAAGT TAATGAGATT AGTGAATGTT
    GAAGATATGA AGAAGAAGCT TATTGGTATG GGTGATAAAG AAATGGTTAC TTACACTACA
    CTTATTGAAG CATCTCAAGG TTTAGGTATC GCTAGATCTC TTGATGAAGC TCATGCTTTT
    GCTCGTGTTC TTGATGATGC TGGTGTTATT TTGATCTTTC GTGATAAAGT CTATCTTCAT
    CCTCATAAGG TGGTGGATTT GATTAGAAAG GCAGTGCCTT TAGGTTTGAA TCCAGATGAT
    GAGTTGATTA GAGAAGAGTT TGATAAGATG AGAAGTATGA AGGAAGAGAT TGATGTGTTA
    GCTCATCAAC AAGTGAGGAA GATTCTATGG GGTGGTCTTG GTTACTCAGT GGTTCAAATT
    GGAATCTTTG TTAGGCTAAC TTTTTGGGAG TTTTCTTGGG ATGTGATGGA ACCAATAACG
    TTTTTCACTA CAGCTACTGG GATTATTGCA GAGGAAGTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077733.1
    CDS199..843
    Translation

    Target ORF information:

    RefSeq Version NM_001084264.2
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana calcium uniporter (DUF607) (AT1G57610), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001084264.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTGGTCGG TGATGGGTTT AGTGAGACGT ACGGCTATGT CCTCCACCGT GAATAAAGCT 
    TCTCCGGTTA GGTCTTTGCT TGGTGGGTTT AGATGTCTCA ACGTTGAGTC TAAGGAAGAA
    GATGAGAAAA AGGATATGAC TGTGTTAGAA GCTAAGAAGT TAATGAGATT AGTGAATGTT
    GAAGATATGA AGAAGAAGCT TATTGGTATG GGTGATAAAG AAATGGTTAC TTACACTACA
    CTTATTGAAG CATCTCAAGG TTTAGGTATC GCTAGATCTC TTGATGAAGC TCATGCTTTT
    GCTCGTGTTC TTGATGATGC TGGTGTTATT TTGATCTTTC GTGATAAAGT CTATCTTCAT
    CCTCATAAGG TGGTGGATTT GATTAGAAAG GCAGTGCCTT TAGGTTTGAA TCCAGATGAT
    GAGTTGATTA GAGAAGAGTT TGATAAGATG AGAAGTATGA AGGAAGAGAT TGATGTGTTA
    GCTCATCAAC AAGTGAGGAA GATTCTATGG GGTGGTCTTG GTTACTCAGT GGTTCAAATT
    GGAATCTTTG TTAGGCTAAC TTTTTGGGAG TTTTCTTGGG ATGTGATGGA ACCAATAACG
    TTTTTCACTA CAGCTACTGG GATTATTGCA GAGGAAGTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb04073
    Clone ID Related Accession (Same CDS sequence) NM_104558.4 , NM_202314.3
    Accession Version NM_104558.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product calcium uniporter (DUF607)
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_104558.3.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTGGTCGG TGATGGGTTT AGTGAGACGT ACGGCTATGT CCTCCACCGT GAATAAAGCT 
    TCTCCGGTTA GGTCTTTGCT TGGTGGGTTT AGATGTCTCA ACGTTGAGTC TAAGGAAGAA
    GATGAGAAAA AGGATATGAC TGTGTTAGAA GCTAAGAAGT TAATGAGATT AGTGAATGTT
    GAAGATATGA AGAAGAAGCT TATTGGTATG GGTGATAAAG AAATGGTTAC TTACACTACA
    CTTATTGAAG CATCTCAAGG TTTAGGTATC GCTAGATCTC TTGATGAAGC TCATGCTTTT
    GCTCGTGTTC TTGATGATGC TGGTGTTATT TTGATCTTTC GTGATAAAGT CTATCTTCAT
    CCTCATAAGG TGGTGGATTT GATTAGAAAG GCAGTGCCTT TAGGTTTGAA TCCAGATGAT
    GAGTTGATTA GAGAAGAGTT TGATAAGATG AGAAGTATGA AGGAAGAGAT TGATGTGTTA
    GCTCATCAAC AAGTGAGGAA GATTCTATGG GGTGGTCTTG GTTACTCAGT GGTTCAAATT
    GGAATCTTTG TTAGGCTAAC TTTTTGGGAG TTTTCTTGGG ATGTGATGGA ACCAATAACG
    TTTTTCACTA CAGCTACTGG GATTATTGTG GGTTATGCTT ATTTCTTGAT GACTTCTAGA
    GATCCAACGT ATCAAGATTT CATGAAAAGG TTGTTTCTTT CCAGGCAGAG GAAGTTGCTT
    AAGAGTCATA AGTTTGATGC TGAGAGGTTT AAAGAACTGG AGAACAAGTG GAAGATAACG
    TCTTGCTCTT CTTCTTCGTG CCACGCTAAT GCATCGATTC GGAATCGTGT TGGTGTTGAT
    CTTGATTTGG AGGATTCTTT GCAGAGTCAT CACAGAGATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_176074.2
    CDS623..1504
    Translation

    Target ORF information:

    RefSeq Version NM_104558.4
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana calcium uniporter (DUF607) (AT1G57610), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_104558.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTGGTCGG TGATGGGTTT AGTGAGACGT ACGGCTATGT CCTCCACCGT GAATAAAGCT 
    TCTCCGGTTA GGTCTTTGCT TGGTGGGTTT AGATGTCTCA ACGTTGAGTC TAAGGAAGAA
    GATGAGAAAA AGGATATGAC TGTGTTAGAA GCTAAGAAGT TAATGAGATT AGTGAATGTT
    GAAGATATGA AGAAGAAGCT TATTGGTATG GGTGATAAAG AAATGGTTAC TTACACTACA
    CTTATTGAAG CATCTCAAGG TTTAGGTATC GCTAGATCTC TTGATGAAGC TCATGCTTTT
    GCTCGTGTTC TTGATGATGC TGGTGTTATT TTGATCTTTC GTGATAAAGT CTATCTTCAT
    CCTCATAAGG TGGTGGATTT GATTAGAAAG GCAGTGCCTT TAGGTTTGAA TCCAGATGAT
    GAGTTGATTA GAGAAGAGTT TGATAAGATG AGAAGTATGA AGGAAGAGAT TGATGTGTTA
    GCTCATCAAC AAGTGAGGAA GATTCTATGG GGTGGTCTTG GTTACTCAGT GGTTCAAATT
    GGAATCTTTG TTAGGCTAAC TTTTTGGGAG TTTTCTTGGG ATGTGATGGA ACCAATAACG
    TTTTTCACTA CAGCTACTGG GATTATTGTG GGTTATGCTT ATTTCTTGAT GACTTCTAGA
    GATCCAACGT ATCAAGATTT CATGAAAAGG TTGTTTCTTT CCAGGCAGAG GAAGTTGCTT
    AAGAGTCATA AGTTTGATGC TGAGAGGTTT AAAGAACTGG AGAACAAGTG GAAGATAACG
    TCTTGCTCTT CTTCTTCGTG CCACGCTAAT GCATCGATTC GGAATCGTGT TGGTGTTGAT
    CTTGATTTGG AGGATTCTTT GCAGAGTCAT CACAGAGATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb04073
    Clone ID Related Accession (Same CDS sequence) NM_104558.4 , NM_202314.3
    Accession Version NM_202314.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product calcium uniporter (DUF607)
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_202314.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTGGTCGG TGATGGGTTT AGTGAGACGT ACGGCTATGT CCTCCACCGT GAATAAAGCT 
    TCTCCGGTTA GGTCTTTGCT TGGTGGGTTT AGATGTCTCA ACGTTGAGTC TAAGGAAGAA
    GATGAGAAAA AGGATATGAC TGTGTTAGAA GCTAAGAAGT TAATGAGATT AGTGAATGTT
    GAAGATATGA AGAAGAAGCT TATTGGTATG GGTGATAAAG AAATGGTTAC TTACACTACA
    CTTATTGAAG CATCTCAAGG TTTAGGTATC GCTAGATCTC TTGATGAAGC TCATGCTTTT
    GCTCGTGTTC TTGATGATGC TGGTGTTATT TTGATCTTTC GTGATAAAGT CTATCTTCAT
    CCTCATAAGG TGGTGGATTT GATTAGAAAG GCAGTGCCTT TAGGTTTGAA TCCAGATGAT
    GAGTTGATTA GAGAAGAGTT TGATAAGATG AGAAGTATGA AGGAAGAGAT TGATGTGTTA
    GCTCATCAAC AAGTGAGGAA GATTCTATGG GGTGGTCTTG GTTACTCAGT GGTTCAAATT
    GGAATCTTTG TTAGGCTAAC TTTTTGGGAG TTTTCTTGGG ATGTGATGGA ACCAATAACG
    TTTTTCACTA CAGCTACTGG GATTATTGTG GGTTATGCTT ATTTCTTGAT GACTTCTAGA
    GATCCAACGT ATCAAGATTT CATGAAAAGG TTGTTTCTTT CCAGGCAGAG GAAGTTGCTT
    AAGAGTCATA AGTTTGATGC TGAGAGGTTT AAAGAACTGG AGAACAAGTG GAAGATAACG
    TCTTGCTCTT CTTCTTCGTG CCACGCTAAT GCATCGATTC GGAATCGTGT TGGTGTTGAT
    CTTGATTTGG AGGATTCTTT GCAGAGTCAT CACAGAGATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_974043.1
    CDS477..1358
    Translation

    Target ORF information:

    RefSeq Version NM_202314.3
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana calcium uniporter (DUF607) (AT1G57610), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_202314.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTGGTCGG TGATGGGTTT AGTGAGACGT ACGGCTATGT CCTCCACCGT GAATAAAGCT 
    TCTCCGGTTA GGTCTTTGCT TGGTGGGTTT AGATGTCTCA ACGTTGAGTC TAAGGAAGAA
    GATGAGAAAA AGGATATGAC TGTGTTAGAA GCTAAGAAGT TAATGAGATT AGTGAATGTT
    GAAGATATGA AGAAGAAGCT TATTGGTATG GGTGATAAAG AAATGGTTAC TTACACTACA
    CTTATTGAAG CATCTCAAGG TTTAGGTATC GCTAGATCTC TTGATGAAGC TCATGCTTTT
    GCTCGTGTTC TTGATGATGC TGGTGTTATT TTGATCTTTC GTGATAAAGT CTATCTTCAT
    CCTCATAAGG TGGTGGATTT GATTAGAAAG GCAGTGCCTT TAGGTTTGAA TCCAGATGAT
    GAGTTGATTA GAGAAGAGTT TGATAAGATG AGAAGTATGA AGGAAGAGAT TGATGTGTTA
    GCTCATCAAC AAGTGAGGAA GATTCTATGG GGTGGTCTTG GTTACTCAGT GGTTCAAATT
    GGAATCTTTG TTAGGCTAAC TTTTTGGGAG TTTTCTTGGG ATGTGATGGA ACCAATAACG
    TTTTTCACTA CAGCTACTGG GATTATTGTG GGTTATGCTT ATTTCTTGAT GACTTCTAGA
    GATCCAACGT ATCAAGATTT CATGAAAAGG TTGTTTCTTT CCAGGCAGAG GAAGTTGCTT
    AAGAGTCATA AGTTTGATGC TGAGAGGTTT AAAGAACTGG AGAACAAGTG GAAGATAACG
    TCTTGCTCTT CTTCTTCGTG CCACGCTAAT GCATCGATTC GGAATCGTGT TGGTGTTGAT
    CTTGATTTGG AGGATTCTTT GCAGAGTCAT CACAGAGATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
    Nature408(6814)816-20(2000 Dec)
    Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW