TOC33 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOC33
Entrez Gene ID	839248
Full Name	translocon at the outer envelope membrane of chloroplasts 33
Synonyms	ATTOC33,PLASTID PROTEIN IMPORT 1,PPI1,T6A9.32,translocon at the outer envelope membrane of chloroplasts 33
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001123743.2	NP_001117215.1	translocon at the outer envelope membrane of chloroplasts 33
NM_100108.5	NP_171730.1	translocon at the outer envelope membrane of chloroplasts 33

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Toc64 participates in chloroplast protein import and cooperates in part with Toc33.
Title: Toc33 and Toc64-III cooperate in precursor protein import into the chloroplasts of Arabidopsis thaliana.

[review] Homodimerization of Toc33 prevents nucleotide exchange, thereby locking the receptor in the guanosine diphosphate (GDP)-loaded state and preventing further activity.
Title: Dimerization of TOC receptor GTPases and its implementation for the control of protein import into chloroplasts.

Pre-protein binding induces a dissociation of the Toc33 homodimer and results in exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP).
Title: Substrate binding disrupts dimerization and induces nucleotide exchange of the chloroplast GTPase Toc33.

Neither nucleotide binding nor dimerization at atToc33 is essential for chloroplast import.
Title: Nucleotide binding and dimerization at the chloroplast pre-protein import receptor, atToc33, are not essential in vivo but do increase import efficiency.

Toc33-R130A mutation reduces homodimerization and affects the interaction of Toc33 with Toc159 during protein import into chloroplasts.
Title: Toc receptor dimerization participates in the initiation of membrane translocation during protein import into chloroplasts.

The following TOC33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOC33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb24576	NM_100108.5 Latest version!	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 33 (TOC33), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OAb24576	NM_001123743.2 Latest version!	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 33 (TOC33), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOC33 ( NM_001123743.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001117215.1 Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 33 (TOC33), mRNA.

Price & Availability↑

CloneID	OAb24576
Clone ID Related Accession (Same CDS sequence)	NM_001123743.2 , NM_100108.5
Accession Version	NM_001123743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	translocon at the outer envelope membrane of chloroplasts 33
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_001123743.1.

ATGGGGTCTC TCGTTCGTGA ATGGGTTGGG TTTCAGCAAT TTCCAGCAGC TACCCAGGAA 
AAGCTGATTG AATTCTTCGG AAAATTGAAG CAAAAGGATA TGAACTCAAT GACAGTTCTT 
GTTCTGGGTA AAGGCGGTGT CGGAAAATCA TCCACTGTCA ATTCTCTTAT CGGCGAACAA 
GTCGTCCGTG TCAGTCCTTT CCAAGCTGAA GGATTGAGAC CAGTGATGGT TTCAAGAACA 
ATGGGAGGGT TCACTATCAA CATTATTGAC ACCCCTGGAC TTGTGGAAGC TGGATATGTC 
AATCACCAAG CTCTCGAGTT AATCAAAGGG TTTCTTGTGA ACAGAACAAT AGATGTCTTG 
CTCTATGTTG ATCGTTTGGA TGTGTATAGA GTCGATGAGC TAGATAAGCA AGTTGTTATA 
GCAATCACCC AAACCTTTGG AAAAGAGATA TGGTGCAAAA CCTTGCTTGT TCTGACTCAT 
GCTCAATTTT CCCCTCCCGA TGAACTTTCC TACGAAACTT TTTCCTCCAA GAGATCAGAT 
TCTCTCCTCA AAACTATCCG GGCTGGTTCT AAGATGCGAA AACAAGAGTT TGAGGATTCT 
GCAATCGCGG TAGTCTATGC AGAGAACAGC GGAAGATGCA GCAAGAACGA TAAGGACGAA 
AAGGCTCTTC CAAATGGTGA AGCGTGGATC CCGAACTTGG TTAAGGCGAT AACTGATGTA 
GCAACAAACC AGAGGAAAGC AATTCATGTA GACAAGAAGA TGGTAGATGG ATCTTACTCT 
GATGATAAAG GAAAGAAACT CATCCCTCTT ATCATAGGCG CTCAGTATTT GATCGTTAAG 
ATGATTCAAG GAGCAATCAG AAATGATATC AAGACAAGTG GAAAGCCACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001117215.1
CDS	72..965
Translation	MGSLVREWVGFQQFPAATQEKLIEFFGKLKQKDMNSMTVLVLGKGGVGKSSTVNSLIGEQVVRVSPFQAEGLRPVMVSRTMGGFTINIIDTPGLVEAGYVNHQALELIKGFLVNRTIDVLLYVDRLDVYRVDELDKQVVIAITQTFGKEIWCKTLLVLTHAQFSPPDELSYETFSSKRSDSLLKTIRAGSKMRKQEFEDSAIAVVYAENSGRCSKNDKDEKALPNGEAWIPNLVKAITDVATNQRKAIHVDKKMVDGSYSDDKGKKLIPLIIGAQYLIVKMIQGAIRNDIKTSGKPL

Target ORF information:

RefSeq Version	NM_001123743.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 33 (TOC33), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123743.2

ORF Insert Sequence:

ATGGGGTCTC TCGTTCGTGA ATGGGTTGGG TTTCAGCAAT TTCCAGCAGC TACCCAGGAA 
AAGCTGATTG AATTCTTCGG AAAATTGAAG CAAAAGGATA TGAACTCAAT GACAGTTCTT 
GTTCTGGGTA AAGGCGGTGT CGGAAAATCA TCCACTGTCA ATTCTCTTAT CGGCGAACAA 
GTCGTCCGTG TCAGTCCTTT CCAAGCTGAA GGATTGAGAC CAGTGATGGT TTCAAGAACA 
ATGGGAGGGT TCACTATCAA CATTATTGAC ACCCCTGGAC TTGTGGAAGC TGGATATGTC 
AATCACCAAG CTCTCGAGTT AATCAAAGGG TTTCTTGTGA ACAGAACAAT AGATGTCTTG 
CTCTATGTTG ATCGTTTGGA TGTGTATAGA GTCGATGAGC TAGATAAGCA AGTTGTTATA 
GCAATCACCC AAACCTTTGG AAAAGAGATA TGGTGCAAAA CCTTGCTTGT TCTGACTCAT 
GCTCAATTTT CCCCTCCCGA TGAACTTTCC TACGAAACTT TTTCCTCCAA GAGATCAGAT 
TCTCTCCTCA AAACTATCCG GGCTGGTTCT AAGATGCGAA AACAAGAGTT TGAGGATTCT 
GCAATCGCGG TAGTCTATGC AGAGAACAGC GGAAGATGCA GCAAGAACGA TAAGGACGAA 
AAGGCTCTTC CAAATGGTGA AGCGTGGATC CCGAACTTGG TTAAGGCGAT AACTGATGTA 
GCAACAAACC AGAGGAAAGC AATTCATGTA GACAAGAAGA TGGTAGATGG ATCTTACTCT 
GATGATAAAG GAAAGAAACT CATCCCTCTT ATCATAGGCG CTCAGTATTT GATCGTTAAG 
ATGATTCAAG GAGCAATCAG AAATGATATC AAGACAAGTG GAAAGCCACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOC33 ( NM_100108.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_171730.1 Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 33 (TOC33), mRNA.

Price & Availability↑

CloneID	OAb24576
Clone ID Related Accession (Same CDS sequence)	NM_001123743.2 , NM_100108.5
Accession Version	NM_100108.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	translocon at the outer envelope membrane of chloroplasts 33
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_100108.4.

ATGGGGTCTC TCGTTCGTGA ATGGGTTGGG TTTCAGCAAT TTCCAGCAGC TACCCAGGAA 
AAGCTGATTG AATTCTTCGG AAAATTGAAG CAAAAGGATA TGAACTCAAT GACAGTTCTT 
GTTCTGGGTA AAGGCGGTGT CGGAAAATCA TCCACTGTCA ATTCTCTTAT CGGCGAACAA 
GTCGTCCGTG TCAGTCCTTT CCAAGCTGAA GGATTGAGAC CAGTGATGGT TTCAAGAACA 
ATGGGAGGGT TCACTATCAA CATTATTGAC ACCCCTGGAC TTGTGGAAGC TGGATATGTC 
AATCACCAAG CTCTCGAGTT AATCAAAGGG TTTCTTGTGA ACAGAACAAT AGATGTCTTG 
CTCTATGTTG ATCGTTTGGA TGTGTATAGA GTCGATGAGC TAGATAAGCA AGTTGTTATA 
GCAATCACCC AAACCTTTGG AAAAGAGATA TGGTGCAAAA CCTTGCTTGT TCTGACTCAT 
GCTCAATTTT CCCCTCCCGA TGAACTTTCC TACGAAACTT TTTCCTCCAA GAGATCAGAT 
TCTCTCCTCA AAACTATCCG GGCTGGTTCT AAGATGCGAA AACAAGAGTT TGAGGATTCT 
GCAATCGCGG TAGTCTATGC AGAGAACAGC GGAAGATGCA GCAAGAACGA TAAGGACGAA 
AAGGCTCTTC CAAATGGTGA AGCGTGGATC CCGAACTTGG TTAAGGCGAT AACTGATGTA 
GCAACAAACC AGAGGAAAGC AATTCATGTA GACAAGAAGA TGGTAGATGG ATCTTACTCT 
GATGATAAAG GAAAGAAACT CATCCCTCTT ATCATAGGCG CTCAGTATTT GATCGTTAAG 
ATGATTCAAG GAGCAATCAG AAATGATATC AAGACAAGTG GAAAGCCACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_171730.1
CDS	79..972
Translation	MGSLVREWVGFQQFPAATQEKLIEFFGKLKQKDMNSMTVLVLGKGGVGKSSTVNSLIGEQVVRVSPFQAEGLRPVMVSRTMGGFTINIIDTPGLVEAGYVNHQALELIKGFLVNRTIDVLLYVDRLDVYRVDELDKQVVIAITQTFGKEIWCKTLLVLTHAQFSPPDELSYETFSSKRSDSLLKTIRAGSKMRKQEFEDSAIAVVYAENSGRCSKNDKDEKALPNGEAWIPNLVKAITDVATNQRKAIHVDKKMVDGSYSDDKGKKLIPLIIGAQYLIVKMIQGAIRNDIKTSGKPL

Target ORF information:

RefSeq Version	NM_100108.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana translocon at the outer envelope membrane of chloroplasts 33 (TOC33), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_100108.5

ORF Insert Sequence:

ATGGGGTCTC TCGTTCGTGA ATGGGTTGGG TTTCAGCAAT TTCCAGCAGC TACCCAGGAA 
AAGCTGATTG AATTCTTCGG AAAATTGAAG CAAAAGGATA TGAACTCAAT GACAGTTCTT 
GTTCTGGGTA AAGGCGGTGT CGGAAAATCA TCCACTGTCA ATTCTCTTAT CGGCGAACAA 
GTCGTCCGTG TCAGTCCTTT CCAAGCTGAA GGATTGAGAC CAGTGATGGT TTCAAGAACA 
ATGGGAGGGT TCACTATCAA CATTATTGAC ACCCCTGGAC TTGTGGAAGC TGGATATGTC 
AATCACCAAG CTCTCGAGTT AATCAAAGGG TTTCTTGTGA ACAGAACAAT AGATGTCTTG 
CTCTATGTTG ATCGTTTGGA TGTGTATAGA GTCGATGAGC TAGATAAGCA AGTTGTTATA 
GCAATCACCC AAACCTTTGG AAAAGAGATA TGGTGCAAAA CCTTGCTTGT TCTGACTCAT 
GCTCAATTTT CCCCTCCCGA TGAACTTTCC TACGAAACTT TTTCCTCCAA GAGATCAGAT 
TCTCTCCTCA AAACTATCCG GGCTGGTTCT AAGATGCGAA AACAAGAGTT TGAGGATTCT 
GCAATCGCGG TAGTCTATGC AGAGAACAGC GGAAGATGCA GCAAGAACGA TAAGGACGAA 
AAGGCTCTTC CAAATGGTGA AGCGTGGATC CCGAACTTGG TTAAGGCGAT AACTGATGTA 
GCAACAAACC AGAGGAAAGC AATTCATGTA GACAAGAAGA TGGTAGATGG ATCTTACTCT 
GATGATAAAG GAAAGAAACT CATCCCTCTT ATCATAGGCG CTCAGTATTT GATCGTTAAG 
ATGATTCAAG GAGCAATCAG AAATGATATC AAGACAAGTG GAAAGCCACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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TOC33 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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