NADK2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NADK2
Entrez Gene ID	838766
Full Name	NAD kinase 2
Synonyms	ATNADK2,F8K7.5,F8K7_5,NAD KINASE 2,NAD kinase 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_102013.5	NP_564145.1	NAD kinase 2
NM_001198128.1	NP_001185057.1	NAD kinase 2

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os11g0191400	NP_001067415.1
Arabidopsis thaliana (thale cress)	NADK2	NP_564145.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The subcellular distribution of the three NADK isoforms was examined using NADK-green fluorescent protein (GFP) fusion proteins expressed transiently in Arabidopsis suspension-cultured cells.
Title: Subcellular and tissue localization of NAD kinases from Arabidopsis: compartmentalization of de novo NADP biosynthesis.

These results indicate that chloroplast NAD kinase (nadk2 gene), catalyzing phosphorylation of NAD, is essential for the proper photosynthetic machinery of PSII and the xanthophyll cycle.
Title: Chloroplast NAD kinase is essential for energy transduction through the xanthophyll cycle in photosynthesis.

results suggest that NADK2 may be a chloroplast NAD kinase and play a vital role in chlorophyll synthesis and chloroplast protection against oxidative damage
Title: NADK2, an Arabidopsis chloroplastic NAD kinase, plays a vital role in both chlorophyll synthesis and chloroplast protection.

The following NADK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NADK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb24159	NM_102013.5 Latest version!	Arabidopsis thaliana NAD kinase 2 (NADK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb30741	NM_001198128.1 Latest version!	Arabidopsis thaliana NAD kinase 2 (NADK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NADK2 ( NM_102013.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_564145.1 Arabidopsis thaliana NAD kinase 2 (NADK2), mRNA.

Price & Availability↑

CloneID	OAb24159
Clone ID Related Accession (Same CDS sequence)	NM_102013.5
Accession Version	NM_102013.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NAD kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_102013.4.

ATGTTCCTAT GCTTTTGCCC TTGCCACGTA CCTATCATGA GTCGCCTTTC TCCGGCCACC 
GGAATCTCTT CCCGCCTCCG CTTCTCCATT GGTTTGAGTT CCGATGGACG ATTAATTCCC 
TTCGGATTCC GGTTTCGGAG AAACGATGTC CCGTTTAAAC GCCGCTTGAG ATTTGTGATC 
AGAGCGCAGC TCTCTGAAGC TTTTTCTCCC GATTTAGGTT TGGATTCTCA GGCTGTGAAA 
TCCCGCGATA CATCAAACTT GCCTTGGATT GGTCCAGTTC CAGGGGACAT TGCTGAGGTT 
GAGGCGTATT GTAGAATTTT TAGATCAGCT GAGCGACTAC ATGGAGCGTT GATGGAGACA 
CTATGCAACC CTGTGACTGG TGAATGTCGA GTACCGTATG ATTTCTCACC GGAGGAAAAA 
CCATTGTTGG AGGACAAAAT AGTATCAGTG CTTGGTTGTA TATTATCTCT TTTAAACAAA 
GGAAGGAAAG AAATTCTCTC TGGGAGGTCA TCTTCTATGA ATTCATTTAA TTTGGATGAT 
GTTGGGGTTG CAGAGGAGTC GCTTCCACCA CTTGCTGTTT TCAGGGGTGA AATGAAACGG 
TGTTGTGAAA GCTTACACAT TGCTCTTGAG AATTATCTGA CGCCGGATGA TGAGAGAAGT 
GGAATTGTTT GGAGGAAATT ACAGAAGCTT AAAAATGTCT GCTACGACGC TGGTTTTCCA 
CGCAGTGATA ACTATCCTTG TCAAACACTT TTTGCGAATT GGGACCCTAT TTACTCGTCA 
AATACGAAAG AGGATATTGA TTCCTACGAG TCTGAGATTG CATTTTGGAG GGGAGGACAG 
GTAACCCAAG AAGGATTGAA GTGGTTGATA GAAAACGGAT TTAAAACAAT TGTTGACCTG 
AGAGCTGAAA TCGTTAAGGA TACATTCTAC CAGACGGCAC TTGATGATGC AATTTCCCTT 
GGGAAAATTA CAGTGGTGCA AATTCCAATT GATGTCAGGA TGGCTCCTAA AGCTGAGCAG 
GTCGAGCTGT TTGCTTCTAT TGTATCAGAT AGCAGCAAAA GACCGATATA TGTTCACAGT 
AAAGAAGGTG TTTGGAGAAC TTCTGCGATG GTTTCTAGGT GGAAGCAGTA CATGACACGA 
CCGATCACGA AAGAAATTCC AGTTTCAGAA GAGTCAAAGC GTCGGGAGGT TTCTGAAACT 
AAGCTTGGAT CAAATGCTGT AGTATCTGGT AAGGGTGTAC CTGATGAGCA GACTGATAAA 
GTCTCTGAAA TCAATGAGGT TGATAGTAGA TCTGCTTCAA GCCAGAGCAA GGAATCTGGA 
AGGTTTGAGG GAGATACATC TGCATCAGAA TTTAATATGG TGAGCGATCC TCTTAAATCT 
CAAGTTCCAC CAGGCAATAT TTTTTCAAGA AAAGAAATGT CTAAATTCCT GAAGAGCAAA 
AGTATTGCTC CTGCTGGTTA TCTTACTAAT CCGTCCAAAA TATTGGGAAC AGTGCCAACT 
CCACAATTTT CATATACTGG TGTGACAAAC GGAAATCAGA TTGTTGATAA AGATTCGATA 
AGAAGACTTG CGGAGACAGG AAACTCCAAT GGGACCCTTC TACCTACAAG TTCTCAAAGT 
TTAGATTTTG GCAACGGGAA GTTTTCAAAT GGAAATGTGC ATGCGTCTGA TAACACCAAC 
AAAAGTATAT CGGACAACAG GGGAAATGGC TTCTCTGCAG CCCCTATTGC TGTGCCTCCT 
AGTGATAACT TAAGTCGCGC TGTAGGATCC CATTCGGTTC GAGAGTCTCA GACTCAGAGA 
AATAATAGTG GTTCCTCCTC GGATTCCAGT GATGATGAAG CTGGAGCTAT TGAGGGAAAT 
ATGTGTGCTT CTGCCACTGG TGTAGTAAGG GTGCAGTCGA GAAAGAAAGC AGAGATGTTC 
TTAGTCCGAA CTGATGGAGT GTCTTGTACA AGGGAAAAGG TGACAGAATC CTCTCTGGCC 
TTCACACATC CAAGTACTCA ACAGCAGATG CTTCTTTGGA AAACTACCCC AAAAACTGTC 
TTACTGCTGA AGAAGCTCGG GCAAGAACTG ATGGAGGAAG CTAAAGAGGC TGCCTCTTTC 
TTGTATCACC AAGAGAATAT GAATGTTCTG GTTGAACCTG AGGTGCATGA TGTATTTGCC 
AGGATTCCAG GGTTTGGCTT TGTCCAGACC TTCTACATTC AGGACACGAG CGATCTCCAT 
GAAAGGGTTG ATTTTGTGGC ATGCTTAGGG GGGGATGGGG TGATATTACA TGCATCAAAC 
TTGTTCAAAG GAGCCGTCCC TCCCGTTGTT TCATTTAATC TGGGGTCTCT TGGATTTCTC 
ACTTCACATC CATTTGAGGA CTTCAGGCAA GACCTCAAAC GAGTCATCCA TGGGAATAAC 
ACGCTAGATG GGGTTTATAT AACTCTTCGA ATGCGTCTTC GTTGCGAAAT CTATCGTAAA 
GGCAAAGCAA TGCCTGGTAA AGTGTTTGAT GTTCTGAACG AGATTGTTGT TGATCGAGGA 
TCCAACCCAT ACCTTTCTAA GATCGAATGT TATGAGCACG ACCGTCTTAT CACGAAGGTA 
CAAGGCGATG GAGTTATAGT AGCCACTCCT ACAGGAAGTA CTGCTTATTC TACAGCAGCA 
GGAGGTTCCA TGGTGCATCC AAACGTTCCT TGCATGCTGT TCACTCCAAT CTGCCCACAT 
TCCCTGTCGT TCAGACCAGT TATACTTCCA GATTCTGCAA AACTCGAGTT AAAGATTCCA 
GATGATGCTC GAAGCAATGC ATGGGTTTCG TTTGATGGAA AGAGAAGACA ACAACTTTCA 
AGGGGAGATT CGGTGAGAAT ATACATGAGC CAACATCCAC TCCCAACTGT CAACAAATCT 
GATCAAACCG GTGATTGGTT TAGAAGCTTA ATCCGTTGCT TAAACTGGAA CGAGCGTCTT 
GATCAAAAGG CTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_564145.1
CDS	138..3095
Translation	MFLCFCPCHVPIMSRLSPATGISSRLRFSIGLSSDGRLIPFGFRFRRNDVPFKRRLRFVIRAQLSEAFSPDLGLDSQAVKSRDTSNLPWIGPVPGDIAEVEAYCRIFRSAERLHGALMETLCNPVTGECRVPYDFSPEEKPLLEDKIVSVLGCILSLLNKGRKEILSGRSSSMNSFNLDDVGVAEESLPPLAVFRGEMKRCCESLHIALENYLTPDDERSGIVWRKLQKLKNVCYDAGFPRSDNYPCQTLFANWDPIYSSNTKEDIDSYESEIAFWRGGQVTQEGLKWLIENGFKTIVDLRAEIVKDTFYQTALDDAISLGKITVVQIPIDVRMAPKAEQVELFASIVSDSSKRPIYVHSKEGVWRTSAMVSRWKQYMTRPITKEIPVSEESKRREVSETKLGSNAVVSGKGVPDEQTDKVSEINEVDSRSASSQSKESGRFEGDTSASEFNMVSDPLKSQVPPGNIFSRKEMSKFLKSKSIAPAGYLTNPSKILGTVPTPQFSYTGVTNGNQIVDKDSIRRLAETGNSNGTLLPTSSQSLDFGNGKFSNGNVHASDNTNKSISDNRGNGFSAAPIAVPPSDNLSRAVGSHSVRESQTQRNNSGSSSDSSDDEAGAIEGNMCASATGVVRVQSRKKAEMFLVRTDGVSCTREKVTESSLAFTHPSTQQQMLLWKTTPKTVLLLKKLGQELMEEAKEAASFLYHQENMNVLVEPEVHDVFARIPGFGFVQTFYIQDTSDLHERVDFVACLGGDGVILHASNLFKGAVPPVVSFNLGSLGFLTSHPFEDFRQDLKRVIHGNNTLDGVYITLRMRLRCEIYRKGKAMPGKVFDVLNEIVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSAKLELKIPDDARSNAWVSFDGKRRQQLSRGDSVRIYMSQHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL

Target ORF information:

RefSeq Version	NM_102013.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NAD kinase 2 (NADK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_102013.5

ORF Insert Sequence:

ATGTTCCTAT GCTTTTGCCC TTGCCACGTA CCTATCATGA GTCGCCTTTC TCCGGCCACC 
GGAATCTCTT CCCGCCTCCG CTTCTCCATT GGTTTGAGTT CCGATGGACG ATTAATTCCC 
TTCGGATTCC GGTTTCGGAG AAACGATGTC CCGTTTAAAC GCCGCTTGAG ATTTGTGATC 
AGAGCGCAGC TCTCTGAAGC TTTTTCTCCC GATTTAGGTT TGGATTCTCA GGCTGTGAAA 
TCCCGCGATA CATCAAACTT GCCTTGGATT GGTCCAGTTC CAGGGGACAT TGCTGAGGTT 
GAGGCGTATT GTAGAATTTT TAGATCAGCT GAGCGACTAC ATGGAGCGTT GATGGAGACA 
CTATGCAACC CTGTGACTGG TGAATGTCGA GTACCGTATG ATTTCTCACC GGAGGAAAAA 
CCATTGTTGG AGGACAAAAT AGTATCAGTG CTTGGTTGTA TATTATCTCT TTTAAACAAA 
GGAAGGAAAG AAATTCTCTC TGGGAGGTCA TCTTCTATGA ATTCATTTAA TTTGGATGAT 
GTTGGGGTTG CAGAGGAGTC GCTTCCACCA CTTGCTGTTT TCAGGGGTGA AATGAAACGG 
TGTTGTGAAA GCTTACACAT TGCTCTTGAG AATTATCTGA CGCCGGATGA TGAGAGAAGT 
GGAATTGTTT GGAGGAAATT ACAGAAGCTT AAAAATGTCT GCTACGACGC TGGTTTTCCA 
CGCAGTGATA ACTATCCTTG TCAAACACTT TTTGCGAATT GGGACCCTAT TTACTCGTCA 
AATACGAAAG AGGATATTGA TTCCTACGAG TCTGAGATTG CATTTTGGAG GGGAGGACAG 
GTAACCCAAG AAGGATTGAA GTGGTTGATA GAAAACGGAT TTAAAACAAT TGTTGACCTG 
AGAGCTGAAA TCGTTAAGGA TACATTCTAC CAGACGGCAC TTGATGATGC AATTTCCCTT 
GGGAAAATTA CAGTGGTGCA AATTCCAATT GATGTCAGGA TGGCTCCTAA AGCTGAGCAG 
GTCGAGCTGT TTGCTTCTAT TGTATCAGAT AGCAGCAAAA GACCGATATA TGTTCACAGT 
AAAGAAGGTG TTTGGAGAAC TTCTGCGATG GTTTCTAGGT GGAAGCAGTA CATGACACGA 
CCGATCACGA AAGAAATTCC AGTTTCAGAA GAGTCAAAGC GTCGGGAGGT TTCTGAAACT 
AAGCTTGGAT CAAATGCTGT AGTATCTGGT AAGGGTGTAC CTGATGAGCA GACTGATAAA 
GTCTCTGAAA TCAATGAGGT TGATAGTAGA TCTGCTTCAA GCCAGAGCAA GGAATCTGGA 
AGGTTTGAGG GAGATACATC TGCATCAGAA TTTAATATGG TGAGCGATCC TCTTAAATCT 
CAAGTTCCAC CAGGCAATAT TTTTTCAAGA AAAGAAATGT CTAAATTCCT GAAGAGCAAA 
AGTATTGCTC CTGCTGGTTA TCTTACTAAT CCGTCCAAAA TATTGGGAAC AGTGCCAACT 
CCACAATTTT CATATACTGG TGTGACAAAC GGAAATCAGA TTGTTGATAA AGATTCGATA 
AGAAGACTTG CGGAGACAGG AAACTCCAAT GGGACCCTTC TACCTACAAG TTCTCAAAGT 
TTAGATTTTG GCAACGGGAA GTTTTCAAAT GGAAATGTGC ATGCGTCTGA TAACACCAAC 
AAAAGTATAT CGGACAACAG GGGAAATGGC TTCTCTGCAG CCCCTATTGC TGTGCCTCCT 
AGTGATAACT TAAGTCGCGC TGTAGGATCC CATTCGGTTC GAGAGTCTCA GACTCAGAGA 
AATAATAGTG GTTCCTCCTC GGATTCCAGT GATGATGAAG CTGGAGCTAT TGAGGGAAAT 
ATGTGTGCTT CTGCCACTGG TGTAGTAAGG GTGCAGTCGA GAAAGAAAGC AGAGATGTTC 
TTAGTCCGAA CTGATGGAGT GTCTTGTACA AGGGAAAAGG TGACAGAATC CTCTCTGGCC 
TTCACACATC CAAGTACTCA ACAGCAGATG CTTCTTTGGA AAACTACCCC AAAAACTGTC 
TTACTGCTGA AGAAGCTCGG GCAAGAACTG ATGGAGGAAG CTAAAGAGGC TGCCTCTTTC 
TTGTATCACC AAGAGAATAT GAATGTTCTG GTTGAACCTG AGGTGCATGA TGTATTTGCC 
AGGATTCCAG GGTTTGGCTT TGTCCAGACC TTCTACATTC AGGACACGAG CGATCTCCAT 
GAAAGGGTTG ATTTTGTGGC ATGCTTAGGG GGGGATGGGG TGATATTACA TGCATCAAAC 
TTGTTCAAAG GAGCCGTCCC TCCCGTTGTT TCATTTAATC TGGGGTCTCT TGGATTTCTC 
ACTTCACATC CATTTGAGGA CTTCAGGCAA GACCTCAAAC GAGTCATCCA TGGGAATAAC 
ACGCTAGATG GGGTTTATAT AACTCTTCGA ATGCGTCTTC GTTGCGAAAT CTATCGTAAA 
GGCAAAGCAA TGCCTGGTAA AGTGTTTGAT GTTCTGAACG AGATTGTTGT TGATCGAGGA 
TCCAACCCAT ACCTTTCTAA GATCGAATGT TATGAGCACG ACCGTCTTAT CACGAAGGTA 
CAAGGCGATG GAGTTATAGT AGCCACTCCT ACAGGAAGTA CTGCTTATTC TACAGCAGCA 
GGAGGTTCCA TGGTGCATCC AAACGTTCCT TGCATGCTGT TCACTCCAAT CTGCCCACAT 
TCCCTGTCGT TCAGACCAGT TATACTTCCA GATTCTGCAA AACTCGAGTT AAAGATTCCA 
GATGATGCTC GAAGCAATGC ATGGGTTTCG TTTGATGGAA AGAGAAGACA ACAACTTTCA 
AGGGGAGATT CGGTGAGAAT ATACATGAGC CAACATCCAC TCCCAACTGT CAACAAATCT 
GATCAAACCG GTGATTGGTT TAGAAGCTTA ATCCGTTGCT TAAACTGGAA CGAGCGTCTT 
GATCAAAAGG CTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NADK2 ( NM_001198128.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185057.1 Arabidopsis thaliana NAD kinase 2 (NADK2), mRNA.

Price & Availability↑

CloneID	OAb30741
Clone ID Related Accession (Same CDS sequence)	NM_001198128.1
Accession Version	NM_001198128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NAD kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGTTCCTAT GCTTTTGCCC TTGCCACGTA CCTATCATGA GTCGCCTTTC TCCGGCCACC 
GGAATCTCTT CCCGCCTCCG CTTCTCCATT GGTTTGAGTT CCGATGGACG ATTAATTCCC 
TTCGGATTCC GGTTTCGGAG AAACGATGTC CCGTTTAAAC GCCGCTTGAG ATTTGTGATC 
AGAGCGCAGC TCTCTGAAGC TTTTTCTCCC GATTTAGGTT TGGATTCTCA GGCTGTGAAA 
TCCCGCGATA CATCAAACTT GCCTTGGATT GGTCCAGTTC CAGGGGACAT TGCTGAGGTT 
GAGGCGTATT GTAGAATTTT TAGATCAGCT GAGCGACTAC ATGGAGCGTT GATGGAGACA 
CTATGCAACC CTGTGACTGG TGAATGTCGA GTACCGTATG ATTTCTCACC GGAGGAAAAA 
CCATTGTTGG AGGACAAAAT AGTATCAGTG CTTGGTTGTA TATTATCTCT TTTAAACAAA 
GGAAGGAAAG AAATTCTCTC TGGGAGGTCA TCTTCTATGA ATTCATTTAA TTTGGATGAT 
GTTGGGGTTG CAGAGGAGTC GCTTCCACCA CTTGCTGTTT TCAGGGGTGA AATGAAACGG 
TGTTGTGAAA GCTTACACAT TGCTCTTGAG AATTATCTGA CGCCGGATGA TGAGAGAAGT 
GGAATTGTTT GGAGGAAATT ACAGAAGCTT AAAAATGTCT GCTACGACGC TGGTTTTCCA 
CGCAGTGATA ACTATCCTTG TCAAACACTT TTTGCGAATT GGGACCCTAT TTACTCGTCA 
AATACGAAAG AGGATATTGA TTCCTACGAG TCTGAGATTG CATTTTGGAG GGGAGGACAG 
GTAACCCAAG AAGGATTGAA GTGGTTGATA GAAAACGGAT TTAAAACAAT TGTTGACCTG 
AGAGCTGAAA TCGTTAAGGA TACATTCTAC CAGACGGCAC TTGATGATGC AATTTCCCTT 
GGGAAAATTA CAGTGGTGCA AATTCCAATT GATGTCAGGA TGGCTCCTAA AGCTGAGCAG 
GTCGAGCTGT TTGCTTCTAT TGTATCAGAT AGCAGCAAAA GACCGATATA TGTTCACAGT 
AAAGAAGGTG TTTGGAGAAC TTCTGCGATG GTTTCTAGGT GGAAGCAGTA CATGACACGA 
CCGATCACGA AAGAAATTCC AGTTTCAGAA GAGTCAAAGC GTCGGGAGGT TTCTGAAACT 
AAGCTTGGAT CAAATGCTGT AGTATCTGGT AAGGGTGTAC CTGATGAGCA GACTGATAAA 
GTCTCTGAAA TCAATGAGGT TGATAGTAGA TCTGCTTCAA GCCAGAGCAA GGAATCTGGA 
AGGTTTGAGG GAGATACATC TGCATCAGAA TTTAATATGG TGAGCGATCC TCTTAAATCT 
CAAGTTCCAC CAGGCAATAT TTTTTCAAGA AAAGAAATGT CTAAATTCCT GAAGAGCAAA 
AGTATTGCTC CTGCTGGTTA TCTTACTAAT CCGTCCAAAA TATTGGGAAC AGTGCCAACT 
CCACAATTTT CATATACTGG TGTGACAAAC GGAAATCAGA TTGTTGATAA AGATTCGATA 
AGAAGACTTG CGGAGACAGG AAACTCCAAT GGGACCCTTC TACCTACAAG TTCTCAAAGT 
TTAGATTTTG GCAACGGGAA GTTTTCAAAT GGAAATGTGC ATGCGTCTGA TAACACCAAC 
AAAAGTATAT CGGACAACAG GGGAAATGGC TTCTCTGCAG CCCCTATTGC TGTGCCTCCT 
AGTGATAACT TAAGTCGCGC TGTAGGATCC CATTCGGTTC GAGAGTCTCA GACTCAGAGA 
AATAATAGTG GTTCCTCCTC GGATTCCAGT GATGATGAAG CTGGAGCTAT TGAGGGAAAT 
ATGTGTGCTT CTGCCACTGG TGTAGTAAGG GTGCAGTCGA GAAAGAAAGC AGAGATGTTC 
TTAGTCCGAA CTGATGGAGT GTCTTGTACA AGGGAAAAGG TGACAGAATC CTCTCTGGCC 
TTCACACATC CAAGTACTCA ACAGCAGATG CTTCTTTGGA AAACTACCCC AAAAACTGTC 
TTACTGCTGA AGAAGCTCGG GCAAGAACTG ATGGAGGAAG CTAAAGAGGA AGTCCATGAA 
AAGTTGTTGG TCTTTCAAAT TTGTTTCCAG GCTGCCTCTT TCTTGTATCA CCAAGAGAAT 
ATGAATGTTC TGGTTGAACC TGAGGTGCAT GATGTATTTG CCAGGATTCC AGGGTTTGGC 
TTTGTCCAGA CCTTCTACAT TCAGGACACG AGCGATCTCC ATGAAAGGGT TGATTTTGTG 
GCATGCTTAG GGGGGGATGG GGTGATATTA CATGCATCAA ACTTGTTCAA AGGAGCCGTC 
CCTCCCGTTG TTTCATTTAA TCTGGGGTCT CTTGGATTTC TCACTTCACA TCCATTTGAG 
GACTTCAGGC AAGACCTCAA ACGAGTCATC CATGGGAATA ACACGCTAGA TGGGGTTTAT 
ATAACTCTTC GAATGCGTCT TCGTTGCGAA ATCTATCGTA AAGGCAAAGC AATGCCTGGT 
AAAGTGTTTG ATGTTCTGAA CGAGATTGTT GTTGATCGAG GATCCAACCC ATACCTTTCT 
AAGATCGAAT GTTATGAGCA CGACCGTCTT ATCACGAAGG TACAAGGCGA TGGAGTTATA 
GTAGCCACTC CTACAGGAAG TACTGCTTAT TCTACAGCAG CAGGAGGTTC CATGGTGCAT 
CCAAACGTTC CTTGCATGCT GTTCACTCCA ATCTGCCCAC ATTCCCTGTC GTTCAGACCA 
GTTATACTTC CAGATTCTGC AAAACTCGAG TTAAAGATTC CAGATGATGC TCGAAGCAAT 
GCATGGGTTT CGTTTGATGG AAAGAGAAGA CAACAACTTT CAAGGGGAGA TTCGGTGAGA 
ATATACATGA GCCAACATCC ACTCCCAACT GTCAACAAAT CTGATCAAAC CGGTGATTGG 
TTTAGAAGCT TAATCCGTTG CTTAAACTGG AACGAGCGTC TTGATCAAAA GGCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185057.1
CDS	313..3312
Translation	MFLCFCPCHVPIMSRLSPATGISSRLRFSIGLSSDGRLIPFGFRFRRNDVPFKRRLRFVIRAQLSEAFSPDLGLDSQAVKSRDTSNLPWIGPVPGDIAEVEAYCRIFRSAERLHGALMETLCNPVTGECRVPYDFSPEEKPLLEDKIVSVLGCILSLLNKGRKEILSGRSSSMNSFNLDDVGVAEESLPPLAVFRGEMKRCCESLHIALENYLTPDDERSGIVWRKLQKLKNVCYDAGFPRSDNYPCQTLFANWDPIYSSNTKEDIDSYESEIAFWRGGQVTQEGLKWLIENGFKTIVDLRAEIVKDTFYQTALDDAISLGKITVVQIPIDVRMAPKAEQVELFASIVSDSSKRPIYVHSKEGVWRTSAMVSRWKQYMTRPITKEIPVSEESKRREVSETKLGSNAVVSGKGVPDEQTDKVSEINEVDSRSASSQSKESGRFEGDTSASEFNMVSDPLKSQVPPGNIFSRKEMSKFLKSKSIAPAGYLTNPSKILGTVPTPQFSYTGVTNGNQIVDKDSIRRLAETGNSNGTLLPTSSQSLDFGNGKFSNGNVHASDNTNKSISDNRGNGFSAAPIAVPPSDNLSRAVGSHSVRESQTQRNNSGSSSDSSDDEAGAIEGNMCASATGVVRVQSRKKAEMFLVRTDGVSCTREKVTESSLAFTHPSTQQQMLLWKTTPKTVLLLKKLGQELMEEAKEEVHEKLLVFQICFQAASFLYHQENMNVLVEPEVHDVFARIPGFGFVQTFYIQDTSDLHERVDFVACLGGDGVILHASNLFKGAVPPVVSFNLGSLGFLTSHPFEDFRQDLKRVIHGNNTLDGVYITLRMRLRCEIYRKGKAMPGKVFDVLNEIVVDRGSNPYLSKIECYEHDRLITKVQGDGVIVATPTGSTAYSTAAGGSMVHPNVPCMLFTPICPHSLSFRPVILPDSAKLELKIPDDARSNAWVSFDGKRRQQLSRGDSVRIYMSQHPLPTVNKSDQTGDWFRSLIRCLNWNERLDQKAL

Target ORF information:

RefSeq Version	NM_001198128.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NAD kinase 2 (NADK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198128.1

ORF Insert Sequence:

ATGTTCCTAT GCTTTTGCCC TTGCCACGTA CCTATCATGA GTCGCCTTTC TCCGGCCACC 
GGAATCTCTT CCCGCCTCCG CTTCTCCATT GGTTTGAGTT CCGATGGACG ATTAATTCCC 
TTCGGATTCC GGTTTCGGAG AAACGATGTC CCGTTTAAAC GCCGCTTGAG ATTTGTGATC 
AGAGCGCAGC TCTCTGAAGC TTTTTCTCCC GATTTAGGTT TGGATTCTCA GGCTGTGAAA 
TCCCGCGATA CATCAAACTT GCCTTGGATT GGTCCAGTTC CAGGGGACAT TGCTGAGGTT 
GAGGCGTATT GTAGAATTTT TAGATCAGCT GAGCGACTAC ATGGAGCGTT GATGGAGACA 
CTATGCAACC CTGTGACTGG TGAATGTCGA GTACCGTATG ATTTCTCACC GGAGGAAAAA 
CCATTGTTGG AGGACAAAAT AGTATCAGTG CTTGGTTGTA TATTATCTCT TTTAAACAAA 
GGAAGGAAAG AAATTCTCTC TGGGAGGTCA TCTTCTATGA ATTCATTTAA TTTGGATGAT 
GTTGGGGTTG CAGAGGAGTC GCTTCCACCA CTTGCTGTTT TCAGGGGTGA AATGAAACGG 
TGTTGTGAAA GCTTACACAT TGCTCTTGAG AATTATCTGA CGCCGGATGA TGAGAGAAGT 
GGAATTGTTT GGAGGAAATT ACAGAAGCTT AAAAATGTCT GCTACGACGC TGGTTTTCCA 
CGCAGTGATA ACTATCCTTG TCAAACACTT TTTGCGAATT GGGACCCTAT TTACTCGTCA 
AATACGAAAG AGGATATTGA TTCCTACGAG TCTGAGATTG CATTTTGGAG GGGAGGACAG 
GTAACCCAAG AAGGATTGAA GTGGTTGATA GAAAACGGAT TTAAAACAAT TGTTGACCTG 
AGAGCTGAAA TCGTTAAGGA TACATTCTAC CAGACGGCAC TTGATGATGC AATTTCCCTT 
GGGAAAATTA CAGTGGTGCA AATTCCAATT GATGTCAGGA TGGCTCCTAA AGCTGAGCAG 
GTCGAGCTGT TTGCTTCTAT TGTATCAGAT AGCAGCAAAA GACCGATATA TGTTCACAGT 
AAAGAAGGTG TTTGGAGAAC TTCTGCGATG GTTTCTAGGT GGAAGCAGTA CATGACACGA 
CCGATCACGA AAGAAATTCC AGTTTCAGAA GAGTCAAAGC GTCGGGAGGT TTCTGAAACT 
AAGCTTGGAT CAAATGCTGT AGTATCTGGT AAGGGTGTAC CTGATGAGCA GACTGATAAA 
GTCTCTGAAA TCAATGAGGT TGATAGTAGA TCTGCTTCAA GCCAGAGCAA GGAATCTGGA 
AGGTTTGAGG GAGATACATC TGCATCAGAA TTTAATATGG TGAGCGATCC TCTTAAATCT 
CAAGTTCCAC CAGGCAATAT TTTTTCAAGA AAAGAAATGT CTAAATTCCT GAAGAGCAAA 
AGTATTGCTC CTGCTGGTTA TCTTACTAAT CCGTCCAAAA TATTGGGAAC AGTGCCAACT 
CCACAATTTT CATATACTGG TGTGACAAAC GGAAATCAGA TTGTTGATAA AGATTCGATA 
AGAAGACTTG CGGAGACAGG AAACTCCAAT GGGACCCTTC TACCTACAAG TTCTCAAAGT 
TTAGATTTTG GCAACGGGAA GTTTTCAAAT GGAAATGTGC ATGCGTCTGA TAACACCAAC 
AAAAGTATAT CGGACAACAG GGGAAATGGC TTCTCTGCAG CCCCTATTGC TGTGCCTCCT 
AGTGATAACT TAAGTCGCGC TGTAGGATCC CATTCGGTTC GAGAGTCTCA GACTCAGAGA 
AATAATAGTG GTTCCTCCTC GGATTCCAGT GATGATGAAG CTGGAGCTAT TGAGGGAAAT 
ATGTGTGCTT CTGCCACTGG TGTAGTAAGG GTGCAGTCGA GAAAGAAAGC AGAGATGTTC 
TTAGTCCGAA CTGATGGAGT GTCTTGTACA AGGGAAAAGG TGACAGAATC CTCTCTGGCC 
TTCACACATC CAAGTACTCA ACAGCAGATG CTTCTTTGGA AAACTACCCC AAAAACTGTC 
TTACTGCTGA AGAAGCTCGG GCAAGAACTG ATGGAGGAAG CTAAAGAGGA AGTCCATGAA 
AAGTTGTTGG TCTTTCAAAT TTGTTTCCAG GCTGCCTCTT TCTTGTATCA CCAAGAGAAT 
ATGAATGTTC TGGTTGAACC TGAGGTGCAT GATGTATTTG CCAGGATTCC AGGGTTTGGC 
TTTGTCCAGA CCTTCTACAT TCAGGACACG AGCGATCTCC ATGAAAGGGT TGATTTTGTG 
GCATGCTTAG GGGGGGATGG GGTGATATTA CATGCATCAA ACTTGTTCAA AGGAGCCGTC 
CCTCCCGTTG TTTCATTTAA TCTGGGGTCT CTTGGATTTC TCACTTCACA TCCATTTGAG 
GACTTCAGGC AAGACCTCAA ACGAGTCATC CATGGGAATA ACACGCTAGA TGGGGTTTAT 
ATAACTCTTC GAATGCGTCT TCGTTGCGAA ATCTATCGTA AAGGCAAAGC AATGCCTGGT 
AAAGTGTTTG ATGTTCTGAA CGAGATTGTT GTTGATCGAG GATCCAACCC ATACCTTTCT 
AAGATCGAAT GTTATGAGCA CGACCGTCTT ATCACGAAGG TACAAGGCGA TGGAGTTATA 
GTAGCCACTC CTACAGGAAG TACTGCTTAT TCTACAGCAG CAGGAGGTTC CATGGTGCAT 
CCAAACGTTC CTTGCATGCT GTTCACTCCA ATCTGCCCAC ATTCCCTGTC GTTCAGACCA 
GTTATACTTC CAGATTCTGC AAAACTCGAG TTAAAGATTC CAGATGATGC TCGAAGCAAT 
GCATGGGTTT CGTTTGATGG AAAGAGAAGA CAACAACTTT CAAGGGGAGA TTCGGTGAGA 
ATATACATGA GCCAACATCC ACTCCCAACT GTCAACAAAT CTGATCAAAC CGGTGATTGG 
TTTAGAAGCT TAATCCGTTG CTTAAACTGG AACGAGCGTC TTGATCAAAA GGCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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NADK2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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