REV1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	REV1
Entrez Gene ID	834504
Full Name	DNA-directed DNA polymerase
Synonyms	ARABIDOPSIS THALIANA HOMOLOG OF REVERSIONLESS 1,ATREV1,K23L20.9,K23L20_9
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_123842.5	NP_199288.4	DNA-directed DNA polymerase
NM_001125901.2	NP_001119373.1	DNA-directed DNA polymerase

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0691000	NP_001058419.1
Arabidopsis thaliana (thale cress)	REV1	NP_199288.4

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mutation frequency in AtPolzeta-, AtRev1- or AtPoleta-knockout plants rev3-1, rev1-1 and polh-1, respectively, were analyzed.
Title: Role of AtPol?, AtRev1 and AtPol? in ? ray-induced mutagenesis.

Arabidopsis thaliana disruptants of AtREV3, AtREV1, and/or AtPOLH genes that encode Translesion synthesis-type polymerases.
Title: Role of AtPol?, AtRev1, and AtPol? in UV light-induced mutagenesis in Arabidopsis.

Transgenic plants that over-expressed or disrupted REV1 showed reduced germination percentage, but the former exhibited a higher stem growth rate than the wild type during development.
Title: Two translesion synthesis DNA polymerase genes, AtPOLH and AtREV1, are involved in development and UV light resistance in Arabidopsis.

A study was conducted to clarify the function of REV1 by in vitro analysis using a primer extension assay.
Title: AtREV1, a Y-family DNA polymerase in Arabidopsis, has deoxynucleotidyl transferase activity in vitro.

A translesion synthesis mechanism exists in higher plants and AtREV1 and AtREV7 have important roles in tolerating exposure to DNA-damaging agents. [AtREV1]
Title: Roles of Arabidopsis AtREV1 and AtREV7 in translesion synthesis.

The following REV1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the REV1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb20557	NM_001125901.2 Latest version!	Arabidopsis thaliana DNA-directed DNA polymerase (REV1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAb20558	NM_123842.5 Latest version!	Arabidopsis thaliana DNA-directed DNA polymerase (REV1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

REV1 ( NM_001125901.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001119373.1 Arabidopsis thaliana DNA-directed DNA polymerase (REV1), mRNA.

Price & Availability↑

CloneID	OAb20557
Clone ID Related Accession (Same CDS sequence)	NM_001125901.2
Accession Version	NM_001125901.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA-directed DNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_001125901.1.

ATGAAGCGTA GCTTGGGTTC AAATTCTTCA AACAATAGTG GCAGTGGAAG TAACAAGAAG 
TCTAAGAAGA ATAATAATCC GAGCAATCAG AAAACCCTAG GCGCGGCATG GGGCGCTGCT 
TCTTCTCGTT CTTCTTTCCG GTCTTCTCCT TTCTCCGATT TCGGAAGTTA CATGGAAGTG 
AAAAATAGGA AACTTCAGAA CCAATTTGAG ACTGAGGCTT CTGCTGCTTC TCGTGGTGTT 
TCGGGTTCTG AGAAACTTAT TTTCCAAGGG GTTTCAATCT TTGTGGATGG GTTCACTATT 
CCTTCTCATC AGGAGTTGAA AGGATACATG ATGAAGTATG GTGGAAGATT TGAGAACTAC 
TTTTCTAGGC GTTCTGTTAC ACATATCATT TGCAGCAACC TTCCAGATAG TAAAGTCAAG 
AATCTTAGGA CATTCAGCAG AGGATTACCT GTTGTGAAAC CTACATGGAT AGTGGACTCT 
ATCTCTGCCA ACAGACTTCT GGGCTGGGTT CCTTACCAGC TAGATCAACT AAATGATACC 
CAGCCAAAGT TGTCTGCCTT TTTTGCACCC AGAAGCCATT TGACCCCTCA GATGGCTTCG 
CCGGTTACAT CTTTTCAGCC TGATACTGGA TACAGTGAAG CTGAAGAAGG AAGTTCAATT 
AGAGCAGATG ATTCAGAGGA AGCAAGGGAC CACATCGATG ATGAAATTGA TGGCGTTTAT 
ATCGAGAACA CTACTCCAGA ACTGACAGAA CAAACAGGAA CTGGTGATCT GAAATCTTCT 
GAAATGAATG CAGAAGGACT TGGTAATTAT GACATAGAAG AAAAAGAAGT TAGTAGTGAA 
CTTCAATCCA CTACTAATCT TCATTCCACT TCAGACAACA AGTCCGTTCA TGCAAATGGG 
AAGAATGGTG GCAAATCAAT TGCAACTGCT GCTGGATCTT CTACAAGGCG TCATTCAACT 
CTCGAGGACC CTAATTTTGT AGAAAACTAC TTTAAGAATT CAAGGCTGCA TTTTATTGGA 
ACATGGAGGA ACAGGTACCG TAAAAGATTT CATGGCTCTT CCAATGGACT TAAATGGGCA 
GATTCAGGCC AGAATACAGC TGAAATGGCC AAGAAGTCAA CCATTATCCA CATTGATTTG 
GATTGCTTCT TTGTGTCAGT GGTAATCAAG AACCGTCTTG AATTACACGA CAAGCCGGTA 
GCTGTATGTC ACTCAGATAA CCCAAAAGGA ACGGCTGAAA TTTCATCTGC AAACTATCCA 
GCGCGGGCTT ATGGAGTGAA GGCAGGTATG TTTGTAAGAC ATGCAAAAGA CCTTTGCCCT 
CAGCTTGTTA TTGTTCCTTA CAACTTTGAA GCCTACGAAG AGGTAGCTGA TCAGTTTTAT 
GACATCTTGC ATAGGCATTG CAGAAAAGTC CAGGCGCTAA GCTGCGATGA AGCGTTTTTA 
GATGTCTCAG ATTTGAGTGA TGTAGAAACC GAAGTTTTAG CCTCGACAAT AAGGAATGAG 
ATCCTTGAGA CTACTGGATG CAGTGCAAGT GCTGGAATAG GCGGTACTAT GCTAATGGCT 
CGTCTTGCTA CCAGAGTTGC TAAACCAGCC GGTCAACTTT ATATTTCTGC TGAGAAGGTA 
GAGGAGTTCT TGGATCAGCT TCCAGTCGGG ACACTACCTG GTGTAGGTTC TGTGTTGAAG 
GAGAAGCTGG TGAAGCAAAA TATTCAAACC TGTGGGCAGT TACGTTTGAT TTCCAAGGAT 
TCTCTTCAAA AGGATTTTGG GGTAAAAACC GGCGAGATGC TTTGGAGTTA TAGCAGAGGA 
CTAGACCTAC GGTCAGTTAC AGCAGTGCAG GAGAGTAAGT CCATAGGGGC TGAAGTGAAT 
TGGGGTGTGC GGTTCAGAGA TCAGCAAGAC GTCTTTATTC TCGTTCAACA CTTCCTCCAA 
TGCCTATGCA AAGAAGTTTC TTTGCGTCTG CAAGGATGTG AAATGATAGG ACGCACTTTT 
ACTCTTAAGA TAAAAAAGAG AAAAAAAGAT GCTGAAGAAC CTACAAAGTA TATGGGTTGT 
GGAGACTGTG ACAATCTGAG TCGCTCCATC ACGGTGCCTG CTGCTACAGA TGACATAGAA 
GTTCTGCAGA GGATTTCAAA GAAGTTGTTT GGGTCATTTT GCTTGGATGT AAAGGAAGTT 
CGTGGGGTTG GGCTTCAGGT CTCTAAGCTT GACAGTGCAG ATCCTTCTAA TAAAGGTTCA 
AGGACTCTGA AATCATGGCT CAGCTCTGCA CCTGCCGTTG TCCAAATAGA ACAAGATGAT 
AATGTTTTTG CTGCAAAGGT TAGGGAAAAC TCAGATTGTA ACAGGCCTGT TACTGGTGGA 
GTTTCTAGAC TCCGAGAATC TAATTCAGAA GAATCTTCAA TTCAGTCAGG TGATACAAAT 
TCATCCTTAC CGCCCATGTG TTATCTCGAC ATGGAAGTTC TAGAGAACCT TCCTCCAGAG 
CTCCTTTCAG AATTAGATGG AACCTACGGT GGGAAGCTGT TTGAGTTGAT TGAGAAAAAA 
CGAGGCAAAA GAAGAATCAA CTGCAACTCT CCACATGTCT CCTTGGATGG CACTGCTGCT 
AGCATTAAAG AATTAAAATC TCTCTCAGTT AAGATACATG GCTTGAGCAC CTCTGGAGAA 
AAGGAATATA AAGAACCATA TGTGCCGCAT CCTTCCATTG CCAGAACGTC TAATCAGCAT 
ACAATAGAAA TGACGGATTT GTTGCCTTCA TCCCTTAGTC AAGTCGACGT CTCTGTGTTA 
CAAGAATTAC CTGAAGAGTT GAGAGCTGAC GTATTGGGAG CATTTCCTTC ACACAGGAGA 
CAACAGTCAT CATCAGATGT TCCCAAGGAG ACTTGTAAGA AACAAGATGA GGAACCAATA 
GATTTAAAGG GCACTGAGAA TGAGATTGGA TTATCATTTA GCTCTCTTTG GTTTGGGAAT 
CCTCCTCTTT GGACAGAGAA GTTCAAAGTT AGTGGCAATT GTACTATGGA AAAACTTTCA 
GCGATATACT TCAAGGTTGC TCAGTCAAGG CCCATGTTAT CCCTAGTCCT GCAACACGCT 
ATTTCAGAAA TGAGTAGTTT TCCTGATGCA GCAAGTGCCA GTGATTTGGA CAAAGCTATT 
TACGATGTGT GTGAACTTCT TAAACAGTAC ATTAACCTCA AGGTAGGAGG TGATATTGAG 
GAGATATATC TCTGCTTCCG ACTTCTGAAA AGATTAGCAG CAAGGAGCCA GCTTTTCCTA 
CAAGTCTATG AGATATTGTC TCCCTTTATT CAGGCCTCGA TCAGTGAACA CTATGGAGGA 
AGCTTGAGTA TACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119373.1
CDS	63..3380
Translation	MKRSLGSNSSNNSGSGSNKKSKKNNNPSNQKTLGAAWGAASSRSSFRSSPFSDFGSYMEVKNRKLQNQFETEASAASRGVSGSEKLIFQGVSIFVDGFTIPSHQELKGYMMKYGGRFENYFSRRSVTHIICSNLPDSKVKNLRTFSRGLPVVKPTWIVDSISANRLLGWVPYQLDQLNDTQPKLSAFFAPRSHLTPQMASPVTSFQPDTGYSEAEEGSSIRADDSEEARDHIDDEIDGVYIENTTPELTEQTGTGDLKSSEMNAEGLGNYDIEEKEVSSELQSTTNLHSTSDNKSVHANGKNGGKSIATAAGSSTRRHSTLEDPNFVENYFKNSRLHFIGTWRNRYRKRFHGSSNGLKWADSGQNTAEMAKKSTIIHIDLDCFFVSVVIKNRLELHDKPVAVCHSDNPKGTAEISSANYPARAYGVKAGMFVRHAKDLCPQLVIVPYNFEAYEEVADQFYDILHRHCRKVQALSCDEAFLDVSDLSDVETEVLASTIRNEILETTGCSASAGIGGTMLMARLATRVAKPAGQLYISAEKVEEFLDQLPVGTLPGVGSVLKEKLVKQNIQTCGQLRLISKDSLQKDFGVKTGEMLWSYSRGLDLRSVTAVQESKSIGAEVNWGVRFRDQQDVFILVQHFLQCLCKEVSLRLQGCEMIGRTFTLKIKKRKKDAEEPTKYMGCGDCDNLSRSITVPAATDDIEVLQRISKKLFGSFCLDVKEVRGVGLQVSKLDSADPSNKGSRTLKSWLSSAPAVVQIEQDDNVFAAKVRENSDCNRPVTGGVSRLRESNSEESSIQSGDTNSSLPPMCYLDMEVLENLPPELLSELDGTYGGKLFELIEKKRGKRRINCNSPHVSLDGTAASIKELKSLSVKIHGLSTSGEKEYKEPYVPHPSIARTSNQHTIEMTDLLPSSLSQVDVSVLQELPEELRADVLGAFPSHRRQQSSSDVPKETCKKQDEEPIDLKGTENEIGLSFSSLWFGNPPLWTEKFKVSGNCTMEKLSAIYFKVAQSRPMLSLVLQHAISEMSSFPDAASASDLDKAIYDVCELLKQYINLKVGGDIEEIYLCFRLLKRLAARSQLFLQVYEILSPFIQASISEHYGGSLSIP

Target ORF information:

RefSeq Version	NM_001125901.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA-directed DNA polymerase (REV1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001125901.2

ORF Insert Sequence:

ATGAAGCGTA GCTTGGGTTC AAATTCTTCA AACAATAGTG GCAGTGGAAG TAACAAGAAG 
TCTAAGAAGA ATAATAATCC GAGCAATCAG AAAACCCTAG GCGCGGCATG GGGCGCTGCT 
TCTTCTCGTT CTTCTTTCCG GTCTTCTCCT TTCTCCGATT TCGGAAGTTA CATGGAAGTG 
AAAAATAGGA AACTTCAGAA CCAATTTGAG ACTGAGGCTT CTGCTGCTTC TCGTGGTGTT 
TCGGGTTCTG AGAAACTTAT TTTCCAAGGG GTTTCAATCT TTGTGGATGG GTTCACTATT 
CCTTCTCATC AGGAGTTGAA AGGATACATG ATGAAGTATG GTGGAAGATT TGAGAACTAC 
TTTTCTAGGC GTTCTGTTAC ACATATCATT TGCAGCAACC TTCCAGATAG TAAAGTCAAG 
AATCTTAGGA CATTCAGCAG AGGATTACCT GTTGTGAAAC CTACATGGAT AGTGGACTCT 
ATCTCTGCCA ACAGACTTCT GGGCTGGGTT CCTTACCAGC TAGATCAACT AAATGATACC 
CAGCCAAAGT TGTCTGCCTT TTTTGCACCC AGAAGCCATT TGACCCCTCA GATGGCTTCG 
CCGGTTACAT CTTTTCAGCC TGATACTGGA TACAGTGAAG CTGAAGAAGG AAGTTCAATT 
AGAGCAGATG ATTCAGAGGA AGCAAGGGAC CACATCGATG ATGAAATTGA TGGCGTTTAT 
ATCGAGAACA CTACTCCAGA ACTGACAGAA CAAACAGGAA CTGGTGATCT GAAATCTTCT 
GAAATGAATG CAGAAGGACT TGGTAATTAT GACATAGAAG AAAAAGAAGT TAGTAGTGAA 
CTTCAATCCA CTACTAATCT TCATTCCACT TCAGACAACA AGTCCGTTCA TGCAAATGGG 
AAGAATGGTG GCAAATCAAT TGCAACTGCT GCTGGATCTT CTACAAGGCG TCATTCAACT 
CTCGAGGACC CTAATTTTGT AGAAAACTAC TTTAAGAATT CAAGGCTGCA TTTTATTGGA 
ACATGGAGGA ACAGGTACCG TAAAAGATTT CATGGCTCTT CCAATGGACT TAAATGGGCA 
GATTCAGGCC AGAATACAGC TGAAATGGCC AAGAAGTCAA CCATTATCCA CATTGATTTG 
GATTGCTTCT TTGTGTCAGT GGTAATCAAG AACCGTCTTG AATTACACGA CAAGCCGGTA 
GCTGTATGTC ACTCAGATAA CCCAAAAGGA ACGGCTGAAA TTTCATCTGC AAACTATCCA 
GCGCGGGCTT ATGGAGTGAA GGCAGGTATG TTTGTAAGAC ATGCAAAAGA CCTTTGCCCT 
CAGCTTGTTA TTGTTCCTTA CAACTTTGAA GCCTACGAAG AGGTAGCTGA TCAGTTTTAT 
GACATCTTGC ATAGGCATTG CAGAAAAGTC CAGGCGCTAA GCTGCGATGA AGCGTTTTTA 
GATGTCTCAG ATTTGAGTGA TGTAGAAACC GAAGTTTTAG CCTCGACAAT AAGGAATGAG 
ATCCTTGAGA CTACTGGATG CAGTGCAAGT GCTGGAATAG GCGGTACTAT GCTAATGGCT 
CGTCTTGCTA CCAGAGTTGC TAAACCAGCC GGTCAACTTT ATATTTCTGC TGAGAAGGTA 
GAGGAGTTCT TGGATCAGCT TCCAGTCGGG ACACTACCTG GTGTAGGTTC TGTGTTGAAG 
GAGAAGCTGG TGAAGCAAAA TATTCAAACC TGTGGGCAGT TACGTTTGAT TTCCAAGGAT 
TCTCTTCAAA AGGATTTTGG GGTAAAAACC GGCGAGATGC TTTGGAGTTA TAGCAGAGGA 
CTAGACCTAC GGTCAGTTAC AGCAGTGCAG GAGAGTAAGT CCATAGGGGC TGAAGTGAAT 
TGGGGTGTGC GGTTCAGAGA TCAGCAAGAC GTCTTTATTC TCGTTCAACA CTTCCTCCAA 
TGCCTATGCA AAGAAGTTTC TTTGCGTCTG CAAGGATGTG AAATGATAGG ACGCACTTTT 
ACTCTTAAGA TAAAAAAGAG AAAAAAAGAT GCTGAAGAAC CTACAAAGTA TATGGGTTGT 
GGAGACTGTG ACAATCTGAG TCGCTCCATC ACGGTGCCTG CTGCTACAGA TGACATAGAA 
GTTCTGCAGA GGATTTCAAA GAAGTTGTTT GGGTCATTTT GCTTGGATGT AAAGGAAGTT 
CGTGGGGTTG GGCTTCAGGT CTCTAAGCTT GACAGTGCAG ATCCTTCTAA TAAAGGTTCA 
AGGACTCTGA AATCATGGCT CAGCTCTGCA CCTGCCGTTG TCCAAATAGA ACAAGATGAT 
AATGTTTTTG CTGCAAAGGT TAGGGAAAAC TCAGATTGTA ACAGGCCTGT TACTGGTGGA 
GTTTCTAGAC TCCGAGAATC TAATTCAGAA GAATCTTCAA TTCAGTCAGG TGATACAAAT 
TCATCCTTAC CGCCCATGTG TTATCTCGAC ATGGAAGTTC TAGAGAACCT TCCTCCAGAG 
CTCCTTTCAG AATTAGATGG AACCTACGGT GGGAAGCTGT TTGAGTTGAT TGAGAAAAAA 
CGAGGCAAAA GAAGAATCAA CTGCAACTCT CCACATGTCT CCTTGGATGG CACTGCTGCT 
AGCATTAAAG AATTAAAATC TCTCTCAGTT AAGATACATG GCTTGAGCAC CTCTGGAGAA 
AAGGAATATA AAGAACCATA TGTGCCGCAT CCTTCCATTG CCAGAACGTC TAATCAGCAT 
ACAATAGAAA TGACGGATTT GTTGCCTTCA TCCCTTAGTC AAGTCGACGT CTCTGTGTTA 
CAAGAATTAC CTGAAGAGTT GAGAGCTGAC GTATTGGGAG CATTTCCTTC ACACAGGAGA 
CAACAGTCAT CATCAGATGT TCCCAAGGAG ACTTGTAAGA AACAAGATGA GGAACCAATA 
GATTTAAAGG GCACTGAGAA TGAGATTGGA TTATCATTTA GCTCTCTTTG GTTTGGGAAT 
CCTCCTCTTT GGACAGAGAA GTTCAAAGTT AGTGGCAATT GTACTATGGA AAAACTTTCA 
GCGATATACT TCAAGGTTGC TCAGTCAAGG CCCATGTTAT CCCTAGTCCT GCAACACGCT 
ATTTCAGAAA TGAGTAGTTT TCCTGATGCA GCAAGTGCCA GTGATTTGGA CAAAGCTATT 
TACGATGTGT GTGAACTTCT TAAACAGTAC ATTAACCTCA AGGTAGGAGG TGATATTGAG 
GAGATATATC TCTGCTTCCG ACTTCTGAAA AGATTAGCAG CAAGGAGCCA GCTTTTCCTA 
CAAGTCTATG AGATATTGTC TCCCTTTATT CAGGCCTCGA TCAGTGAACA CTATGGAGGA 
AGCTTGAGTA TACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

REV1 ( NM_123842.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_199288.4 Arabidopsis thaliana DNA-directed DNA polymerase (REV1), mRNA.

Price & Availability↑

CloneID	OAb20558
Clone ID Related Accession (Same CDS sequence)	NM_123842.5
Accession Version	NM_123842.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA-directed DNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_123842.4.

ATGAAGCGTA GCTTGGGTTC AAATTCTTCA AACAATAGTG GCAGTGGAAG TAACAAGAAG 
TCTAAGAAGA ATAATAATCC GAGCAATCAG AAAACCCTAG GCGCGGCATG GGGCGCTGCT 
TCTTCTCGTT CTTCTTTCCG GTCTTCTCCT TTCTCCGATT TCGGAAGTTA CATGGAAGTG 
AAAAATAGGA AACTTCAGAA CCAATTTGAG ACTGAGGCTT CTGCTGCTTC TCGTGGTGTT 
TCGGGTTCTG AGAAACTTAT TTTCCAAGGG GTTTCAATCT TTGTGGATGG GTTCACTATT 
CCTTCTCATC AGGAGTTGAA AGGATACATG ATGAAGTATG GTGGAAGATT TGAGAACTAC 
TTTTCTAGGC GTTCTGTTAC ACATATCATT TGCAGCAACC TTCCAGATAG TAAAGTCAAG 
AATCTTAGGA CATTCAGCAG AGGATTACCT GTTGTGAAAC CTACATGGAT AGTGGACTCT 
ATCTCTGCCA ACAGACTTCT GGGCTGGGTT CCTTACCAGC TAGATCAACT AAATGATACC 
CAGCCAAAGT TGTCTGCCTT TTTTGCACCC AGAAGCCATT TGACCCCTCA GATGGCTTCG 
CCGGTTACAT CTTTTCAGCC TGATACTGGA TACAGTGAAG CTGAAGAAGG AAGTTCAATT 
AGAGCAGATG ATTCAGAGGA AGCAAGGGAC CACATCGATG ATGAAATTGA TGGCGTTTAT 
ATCGAGAACA CTACTCCAGA ACTGACAGAA CAAACAGGAA CTGGTGATCT GAAATCTTCT 
GAAATGAATG CAGAAGGACT TGGTAATTAT GACATAGAAG AAAAAGAAGT TAGTAGTGAA 
CTTCAATCCA CTACTAATCT TCATTCCACT TCAGACAACA AGTCCGTTCA TGCAAATGGG 
AAGAATGGTG GCAAATCAAT TGCAACTGCT GCTGGATCTT CTACAAGGCG TCATTCAACT 
CTCGAGGACC CTAATTTTGT AGAAAACTAC TTTAAGAATT CAAGGCTGCA TTTTATTGGA 
ACATGGAGGA ACAGGTACCG TAAAAGATTT CATGGCTCTT CCAATGGACT TAAATGGGCA 
GATTCAGGCC AGAATACAGC TGAAATGGCC AAGAAGTCAA CCATTATCCA CATTGATTTG 
GATTGCTTCT TTGTGTCAGT GGTAATCAAG AACCGTCTTG AATTACACGA CAAGCCGGTA 
GCTGTATGTC ACTCAGATAA CCCAAAAGGA ACGGCTGAAA TTTCATCTGC AAACTATCCA 
GCGCGGGCTT ATGGAGTGAA GGCAGGTATG TTTGTAAGAC ATGCAAAAGA CCTTTGCCCT 
CAGCTTGTTA TTGTTCCTTA CAACTTTGAA GCCTACGAAG AGGTAGCTGA TCAGTTTTAT 
GACATCTTGC ATAGGCATTG CAGAAAAGTC CAGGCGCTAA GCTGCGATGA AGCGTTTTTA 
GATGTCTCAG ATTTGAGTGA TGTAGAAACC GAAGTTTTAG CCTCGACAAT AAGGAATGAG 
ATCCTTGAGA CTACTGGATG CAGTGCAAGT GCTGGAATAG GCGGTACTAT GCTAATGGCT 
CGTCTTGCTA CCAGAGTTGC TAAACCAGCC GGTCAACTTT ATATTTCTGC TGAGAAGGTA 
GAGGAGTTCT TGGATCAGCT TCCAGTCGGG ACACTACCTG GTGTAGGTTC TGTGTTGAAG 
GAGAAGCTGG TGAAGCAAAA TATTCAAACC TGTGGGCAGT TACGTTTGAT TTCCAAGGAT 
TCTCTTCAAA AGGATTTTGG GGTAAAAACC GGCGAGATGC TTTGGAGTTA TAGCAGAGGA 
CTAGACCTAC GGTCAGTTAC AGCAGTGCAG GAGAGTAAGT CCATAGGGGC TGAAGTGAAT 
TGGGGTGTGC GGTTCAGAGA TCAGCAAGAC GTTCAACACT TCCTCCAATG CCTATGCAAA 
GAAGTTTCTT TGCGTCTGCA AGGATGTGAA ATGATAGGAC GCACTTTTAC TCTTAAGATA 
AAAAAGAGAA AAAAAGATGC TGAAGAACCT ACAAAGTATA TGGGTTGTGG AGACTGTGAC 
AATCTGAGTC GCTCCATCAC GGTGCCTGCT GCTACAGATG ACATAGAAGT TCTGCAGAGG 
ATTTCAAAGA AGTTGTTTGG GTCATTTTGC TTGGATGTAA AGGAAGTTCG TGGGGTTGGG 
CTTCAGGTCT CTAAGCTTGA CAGTGCAGAT CCTTCTAATA AAGGTTCAAG GACTCTGAAA 
TCATGGCTCA GCTCTGCACC TGCCGTTGTC CAAATAGAAC AAGATGATAA TGTTTTTGCT 
GCAAAGGTTA GGGAAAACTC AGATTGTAAC AGGCCTGTTA CTGGTGGAGT TTCTAGACTC 
CGAGAATCTA ATTCAGAAGA ATCTTCAATT CAGTCAGGTG ATACAAATTC ATCCTTACCG 
CCCATGTGTT ATCTCGACAT GGAAGTTCTA GAGAACCTTC CTCCAGAGCT CCTTTCAGAA 
TTAGATGGAA CCTACGGTGG GAAGCTGTTT GAGTTGATTG AGAAAAAACG AGGCAAAAGA 
AGAATCAACT GCAACTCTCC ACATGTCTCC TTGGATGGCA CTGCTGCTAG CATTAAAGAA 
TTAAAATCTC TCTCAGTTAA GATACATGGC TTGAGCACCT CTGGAGAAAA GGAATATAAA 
GAACCATATG TGCCGCATCC TTCCATTGCC AGAACGTCTA ATCAGCATAC AATAGAAATG 
ACGGATTTGT TGCCTTCATC CCTTAGTCAA GTCGACGTCT CTGTGTTACA AGAATTACCT 
GAAGAGTTGA GAGCTGACGT ATTGGGAGCA TTTCCTTCAC ACAGGAGACA ACAGTCATCA 
TCAGATGTTC CCAAGGAGAC TTGTAAGAAA CAAGATGAGG AACCAATAGA TTTAAAGGGC 
ACTGAGAATG AGATTGGATT ATCATTTAGC TCTCTTTGGT TTGGGAATCC TCCTCTTTGG 
ACAGAGAAGT TCAAAGTTAG TGGCAATTGT ACTATGGAAA AACTTTCAGC GATATACTTC 
AAGGTTGCTC AGTCAAGGCC CATGTTATCC CTAGTCCTGC AACACGCTAT TTCAGAAATG 
AGTAGTTTTC CTGATGCAGC AAGTGCCAGT GATTTGGACA AAGCTATTTA CGATGTGTGT 
GAACTTCTTA AACAGTACAT TAACCTCAAG GTAGGAGGTG ATATTGAGGA GATATATCTC 
TGCTTCCGAC TTCTGAAAAG ATTAGCAGCA AGGAGCCAGC TTTTCCTACA AGTCTATGAG 
ATATTGTCTC CCTTTATTCA GGCCTCGATC AGTGAACACT ATGGAGGAAG CTTGAGTATA 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_199288.4
CDS	63..3368
Translation	MKRSLGSNSSNNSGSGSNKKSKKNNNPSNQKTLGAAWGAASSRSSFRSSPFSDFGSYMEVKNRKLQNQFETEASAASRGVSGSEKLIFQGVSIFVDGFTIPSHQELKGYMMKYGGRFENYFSRRSVTHIICSNLPDSKVKNLRTFSRGLPVVKPTWIVDSISANRLLGWVPYQLDQLNDTQPKLSAFFAPRSHLTPQMASPVTSFQPDTGYSEAEEGSSIRADDSEEARDHIDDEIDGVYIENTTPELTEQTGTGDLKSSEMNAEGLGNYDIEEKEVSSELQSTTNLHSTSDNKSVHANGKNGGKSIATAAGSSTRRHSTLEDPNFVENYFKNSRLHFIGTWRNRYRKRFHGSSNGLKWADSGQNTAEMAKKSTIIHIDLDCFFVSVVIKNRLELHDKPVAVCHSDNPKGTAEISSANYPARAYGVKAGMFVRHAKDLCPQLVIVPYNFEAYEEVADQFYDILHRHCRKVQALSCDEAFLDVSDLSDVETEVLASTIRNEILETTGCSASAGIGGTMLMARLATRVAKPAGQLYISAEKVEEFLDQLPVGTLPGVGSVLKEKLVKQNIQTCGQLRLISKDSLQKDFGVKTGEMLWSYSRGLDLRSVTAVQESKSIGAEVNWGVRFRDQQDVQHFLQCLCKEVSLRLQGCEMIGRTFTLKIKKRKKDAEEPTKYMGCGDCDNLSRSITVPAATDDIEVLQRISKKLFGSFCLDVKEVRGVGLQVSKLDSADPSNKGSRTLKSWLSSAPAVVQIEQDDNVFAAKVRENSDCNRPVTGGVSRLRESNSEESSIQSGDTNSSLPPMCYLDMEVLENLPPELLSELDGTYGGKLFELIEKKRGKRRINCNSPHVSLDGTAASIKELKSLSVKIHGLSTSGEKEYKEPYVPHPSIARTSNQHTIEMTDLLPSSLSQVDVSVLQELPEELRADVLGAFPSHRRQQSSSDVPKETCKKQDEEPIDLKGTENEIGLSFSSLWFGNPPLWTEKFKVSGNCTMEKLSAIYFKVAQSRPMLSLVLQHAISEMSSFPDAASASDLDKAIYDVCELLKQYINLKVGGDIEEIYLCFRLLKRLAARSQLFLQVYEILSPFIQASISEHYGGSLSIP

Target ORF information:

RefSeq Version	NM_123842.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA-directed DNA polymerase (REV1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_123842.5

ORF Insert Sequence:

ATGAAGCGTA GCTTGGGTTC AAATTCTTCA AACAATAGTG GCAGTGGAAG TAACAAGAAG 
TCTAAGAAGA ATAATAATCC GAGCAATCAG AAAACCCTAG GCGCGGCATG GGGCGCTGCT 
TCTTCTCGTT CTTCTTTCCG GTCTTCTCCT TTCTCCGATT TCGGAAGTTA CATGGAAGTG 
AAAAATAGGA AACTTCAGAA CCAATTTGAG ACTGAGGCTT CTGCTGCTTC TCGTGGTGTT 
TCGGGTTCTG AGAAACTTAT TTTCCAAGGG GTTTCAATCT TTGTGGATGG GTTCACTATT 
CCTTCTCATC AGGAGTTGAA AGGATACATG ATGAAGTATG GTGGAAGATT TGAGAACTAC 
TTTTCTAGGC GTTCTGTTAC ACATATCATT TGCAGCAACC TTCCAGATAG TAAAGTCAAG 
AATCTTAGGA CATTCAGCAG AGGATTACCT GTTGTGAAAC CTACATGGAT AGTGGACTCT 
ATCTCTGCCA ACAGACTTCT GGGCTGGGTT CCTTACCAGC TAGATCAACT AAATGATACC 
CAGCCAAAGT TGTCTGCCTT TTTTGCACCC AGAAGCCATT TGACCCCTCA GATGGCTTCG 
CCGGTTACAT CTTTTCAGCC TGATACTGGA TACAGTGAAG CTGAAGAAGG AAGTTCAATT 
AGAGCAGATG ATTCAGAGGA AGCAAGGGAC CACATCGATG ATGAAATTGA TGGCGTTTAT 
ATCGAGAACA CTACTCCAGA ACTGACAGAA CAAACAGGAA CTGGTGATCT GAAATCTTCT 
GAAATGAATG CAGAAGGACT TGGTAATTAT GACATAGAAG AAAAAGAAGT TAGTAGTGAA 
CTTCAATCCA CTACTAATCT TCATTCCACT TCAGACAACA AGTCCGTTCA TGCAAATGGG 
AAGAATGGTG GCAAATCAAT TGCAACTGCT GCTGGATCTT CTACAAGGCG TCATTCAACT 
CTCGAGGACC CTAATTTTGT AGAAAACTAC TTTAAGAATT CAAGGCTGCA TTTTATTGGA 
ACATGGAGGA ACAGGTACCG TAAAAGATTT CATGGCTCTT CCAATGGACT TAAATGGGCA 
GATTCAGGCC AGAATACAGC TGAAATGGCC AAGAAGTCAA CCATTATCCA CATTGATTTG 
GATTGCTTCT TTGTGTCAGT GGTAATCAAG AACCGTCTTG AATTACACGA CAAGCCGGTA 
GCTGTATGTC ACTCAGATAA CCCAAAAGGA ACGGCTGAAA TTTCATCTGC AAACTATCCA 
GCGCGGGCTT ATGGAGTGAA GGCAGGTATG TTTGTAAGAC ATGCAAAAGA CCTTTGCCCT 
CAGCTTGTTA TTGTTCCTTA CAACTTTGAA GCCTACGAAG AGGTAGCTGA TCAGTTTTAT 
GACATCTTGC ATAGGCATTG CAGAAAAGTC CAGGCGCTAA GCTGCGATGA AGCGTTTTTA 
GATGTCTCAG ATTTGAGTGA TGTAGAAACC GAAGTTTTAG CCTCGACAAT AAGGAATGAG 
ATCCTTGAGA CTACTGGATG CAGTGCAAGT GCTGGAATAG GCGGTACTAT GCTAATGGCT 
CGTCTTGCTA CCAGAGTTGC TAAACCAGCC GGTCAACTTT ATATTTCTGC TGAGAAGGTA 
GAGGAGTTCT TGGATCAGCT TCCAGTCGGG ACACTACCTG GTGTAGGTTC TGTGTTGAAG 
GAGAAGCTGG TGAAGCAAAA TATTCAAACC TGTGGGCAGT TACGTTTGAT TTCCAAGGAT 
TCTCTTCAAA AGGATTTTGG GGTAAAAACC GGCGAGATGC TTTGGAGTTA TAGCAGAGGA 
CTAGACCTAC GGTCAGTTAC AGCAGTGCAG GAGAGTAAGT CCATAGGGGC TGAAGTGAAT 
TGGGGTGTGC GGTTCAGAGA TCAGCAAGAC GTTCAACACT TCCTCCAATG CCTATGCAAA 
GAAGTTTCTT TGCGTCTGCA AGGATGTGAA ATGATAGGAC GCACTTTTAC TCTTAAGATA 
AAAAAGAGAA AAAAAGATGC TGAAGAACCT ACAAAGTATA TGGGTTGTGG AGACTGTGAC 
AATCTGAGTC GCTCCATCAC GGTGCCTGCT GCTACAGATG ACATAGAAGT TCTGCAGAGG 
ATTTCAAAGA AGTTGTTTGG GTCATTTTGC TTGGATGTAA AGGAAGTTCG TGGGGTTGGG 
CTTCAGGTCT CTAAGCTTGA CAGTGCAGAT CCTTCTAATA AAGGTTCAAG GACTCTGAAA 
TCATGGCTCA GCTCTGCACC TGCCGTTGTC CAAATAGAAC AAGATGATAA TGTTTTTGCT 
GCAAAGGTTA GGGAAAACTC AGATTGTAAC AGGCCTGTTA CTGGTGGAGT TTCTAGACTC 
CGAGAATCTA ATTCAGAAGA ATCTTCAATT CAGTCAGGTG ATACAAATTC ATCCTTACCG 
CCCATGTGTT ATCTCGACAT GGAAGTTCTA GAGAACCTTC CTCCAGAGCT CCTTTCAGAA 
TTAGATGGAA CCTACGGTGG GAAGCTGTTT GAGTTGATTG AGAAAAAACG AGGCAAAAGA 
AGAATCAACT GCAACTCTCC ACATGTCTCC TTGGATGGCA CTGCTGCTAG CATTAAAGAA 
TTAAAATCTC TCTCAGTTAA GATACATGGC TTGAGCACCT CTGGAGAAAA GGAATATAAA 
GAACCATATG TGCCGCATCC TTCCATTGCC AGAACGTCTA ATCAGCATAC AATAGAAATG 
ACGGATTTGT TGCCTTCATC CCTTAGTCAA GTCGACGTCT CTGTGTTACA AGAATTACCT 
GAAGAGTTGA GAGCTGACGT ATTGGGAGCA TTTCCTTCAC ACAGGAGACA ACAGTCATCA 
TCAGATGTTC CCAAGGAGAC TTGTAAGAAA CAAGATGAGG AACCAATAGA TTTAAAGGGC 
ACTGAGAATG AGATTGGATT ATCATTTAGC TCTCTTTGGT TTGGGAATCC TCCTCTTTGG 
ACAGAGAAGT TCAAAGTTAG TGGCAATTGT ACTATGGAAA AACTTTCAGC GATATACTTC 
AAGGTTGCTC AGTCAAGGCC CATGTTATCC CTAGTCCTGC AACACGCTAT TTCAGAAATG 
AGTAGTTTTC CTGATGCAGC AAGTGCCAGT GATTTGGACA AAGCTATTTA CGATGTGTGT 
GAACTTCTTA AACAGTACAT TAACCTCAAG GTAGGAGGTG ATATTGAGGA GATATATCTC 
TGCTTCCGAC TTCTGAAAAG ATTAGCAGCA AGGAGCCAGC TTTTCCTACA AGTCTATGAG 
ATATTGTCTC CCTTTATTCA GGCCTCGATC AGTGAACACT ATGGAGGAAG CTTGAGTATA 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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REV1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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