DND1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DND1
Entrez Gene ID	831393
Full Name	Cyclic nucleotide-regulated ion channel family protein
Synonyms	ATCNGC2,CNGC2,CYCLIC NUCLEOTIDE GATED CHANNEL 2,CYCLIC NUCLEOTIDE-GATED CHANNEL 2,DEFENSE NO DEATH 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_203054.2	NP_974783.2	Cyclic nucleotide-regulated ion channel family protein
NM_121545.5	NP_197045.1	Cyclic nucleotide-regulated ion channel family protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0758300	NP_001051331.1
Arabidopsis thaliana (thale cress)	DND1	NP_974783.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CNGC2 is the key factor mediating Ca2+ influx into the leaf cells.
Title: CNGC2 Is a Ca2+ Influx Channel That Prevents Accumulation of Apoplastic Ca2+ in the Leaf.

results indicate that S gene function of DND1 is conserved in tomato and potato. We discuss the possibilities of using RNAi silencing or loss-of-function mutations of DND1 orthologs, as well as additional S gene orthologs from Arabidopsis, to breed for resistance to pathogens in crop plants.
Title: Down-regulation of Arabidopsis DND1 orthologs in potato and tomato leads to broad-spectrum resistance to late blight and powdery mildew.

Arabidopsis thaliana cyclic nucleotide-gated channel 2 (AtCNGC2) is involved in jasmonic acid (JA)-induced apoplastic Ca(2+) influx in Arabidopsis epidermal cells.
Title: AtCNGC2 is involved in jasmonic acid-induced calcium mobilization.

This indicates that AtCNGC2 and AtCNGC4 have a unique role controlling flowering timing and this function is independent from its role in pathogen defense.
Title: Crossroads of stress responses, development and flowering regulation--the multiple roles of Cyclic Nucleotide Gated Ion Channel 2.

AtCNGC2 and AtCNGC4 are likely part of the same channel complex.
Title: The Arabidopsis cyclic nucleotide-gated ion channels AtCNGC2 and AtCNGC4 work in the same signaling pathway to regulate pathogen defense and floral transition.

The following DND1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DND1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb18481	NM_121545.5 Latest version!	Arabidopsis thaliana Cyclic nucleotide-regulated ion channel family protein (DND1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb18482	NM_203054.2 Latest version!	Arabidopsis thaliana Cyclic nucleotide-regulated ion channel family protein (DND1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DND1 ( NM_121545.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_197045.1 Arabidopsis thaliana Cyclic nucleotide-regulated ion channel family protein (DND1), mRNA.

Price & Availability↑

CloneID	OAb18481
Clone ID Related Accession (Same CDS sequence)	NM_121545.5
Accession Version	NM_121545.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Cyclic nucleotide-regulated ion channel family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121545.4.

ATGCCCTCTC ACCCCAACTT CATCTTCAGG TGGATTGGAC TGTTTTCCGA TAAGTTCCGT 
CGACAAACGA CTGGGATCGA TGAAAACAGT AACCTCCAAA TCAACGGTGG AGATTCGAGC 
AGCAGCGGCA GCGATGAGAC GCCGGTGCTA AGCTCCGTCG AGTGTTACGC TTGCACACAA 
GTAGGCGTCC CAGCTTTCCA TTCAACTAGC TGCGATCAAG CTCACGCGCC GGAGTGGCGT 
GCCTCCGCCG GCTCTTCTCT AGTTCCGATC CAGGAAGGAT CTGTCCCTAA CCCAGCCCGA 
ACCAGATTCC GACGTCTCAA AGGTCCGTTT GGTGAAGTTC TCGATCCTAG GAGCAAGCGC 
GTGCAGAGAT GGAACCGCGC GTTGCTTTTA GCTCGTGGGA TGGCTTTAGC GGTGGATCCG 
CTCTTCTTCT ACGCGCTTTC CATCGGCCGA ACTACCGGAC CGGCGTGTCT TTACATGGAT 
GGTGCGTTCG CCGCGGTGGT CACGGTGCTC CGCACGTGTC TCGATGCTGT TCATCTTTGG 
CACGTGTGGC TTCAATTCAG ACTGGCCTAC GTCTCGAGAG AGTCGCTTGT CGTTGGTTGT 
GGGAAGCTCG TTTGGGATCC ACGCGCCATC GCGTCTCACT ACGCACGCTC TCTCACTGGC 
TTCTGGTTTG ATGTTATCGT CATCCTCCCT GTCCCTCAGG CAGTGTTTTG GTTAGTTGTG 
CCGAAACTGA TAAGAGAAGA GAAGGTTAAG CTGATAATGA CGATTCTGCT GCTAATATTC 
TTGTTCCAGT TCCTCCCCAA GATTTATCAC TGCATCTGTT TGATGAGAAG GATGCAGAAG 
GTCACTGGTT ACATTTTTGG AACTATTTGG TGGGGTTTTG CTCTTAATCT CATCGCATAT 
TTCATCGCTT CTCATGTTGC TGGGGGATGT TGGTATGTTC TCGCAATACA GCGTGTTGCT 
TCTTGCATAA GACAACAATG TATGAGAACC GGGAACTGCA ATCTGAGTCT GGCTTGCAAA 
GAAGAGGTCT GTTACCAATT TGTGTCACCG ACAAGCACAG TTGGATATCC ATGCTTATCT 
GGAAACCTTA CCAGTGTGGT CAATAAGCCT ATGTGCTTAG ACTCTAACGG ACCATTCCGA 
TATGGTATCT ACCGTTGGGC ACTTCCAGTC ATCTCCAGCA ACTCTCTTGC GGTTAAGATC 
CTTTACCCCA TCTTCTGGGG CCTAATGACT CTCAGCACAT TTGCGAATGA TCTTGAGCCC 
ACAAGCAACT GGCTCGAGGT TATTTTCAGT ATAGTTATGG TTCTAAGTGG CTTGTTACTT 
TTCACGCTGT TGATAGGAAA CATTCAGGTG TTTTTGCATG CGGTAATGGC GAAAAAAAGG 
AAAATGCAGA TACGGTGTAG GGATATGGAA TGGTGGATGA AACGTAGGCA GTTACCTTCC 
CGGTTAAGAC AGAGGGTTAG GCGATTTGAG CGGCAGAGAT GGAATGCCTT GGGTGGTGAA 
GACGAGCTAG AACTTATACA TGATTTGCCT CCGGGTCTTC GAAGAGATAT CAAACGATAT 
CTTTGCTTTG ATCTCATTAA CAAGGTGCCA TTGTTCAGGG GCATGGACGA CTTGATCCTC 
GACAACATTT GCGATCGGGC TAAGCCTCGA GTCTTCTCTA AAGACGAAAA GATCATCCGT 
GAAGGAGATC CTGTACAGAG AATGATATTC ATCATGCGTG GACGAGTCAA ACGTATACAG 
AGCCTAAGCA AAGGCGTCCT AGCCACTAGT ACACTAGAAC CAGGCGGTTA CTTGGGCGAC 
GAGCTACTCT CATGGTGCCT ACGTCGCCCG TTTCTGGACC GTCTTCCCCC TTCCTCAGCA 
ACATTTGTCT GCCTAGAAAA CATCGAGGCA TTCTCCCTCG GATCCGAAGA TCTTAGGTAC 
ATTACCGATC ATTTCCGTTA TAAATTCGCG AACGAGCGGC TTAAGCGGAC CGCAAGATAC 
TATTCCTCAA ACTGGAGGAC GTGGGCAGCG GTAAATATTC AGATGGCGTG GCGCCGGCGT 
AGGAAAAGAA CCCGTGGTGA AAACATCGGC GGTTCGATGA GTCCTGTGTC GGAGAATAGC 
ATTGAAGGTA ACAGTGAACG CCGGTTACTT CAGTATGCAG CTATGTTCAT GTCCATTCGA 
CCGCATGATC ATCTCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_197045.1
CDS	120..2300
Translation	MPSHPNFIFRWIGLFSDKFRRQTTGIDENSNLQINGGDSSSSGSDETPVLSSVECYACTQVGVPAFHSTSCDQAHAPEWRASAGSSLVPIQEGSVPNPARTRFRRLKGPFGEVLDPRSKRVQRWNRALLLARGMALAVDPLFFYALSIGRTTGPACLYMDGAFAAVVTVLRTCLDAVHLWHVWLQFRLAYVSRESLVVGCGKLVWDPRAIASHYARSLTGFWFDVIVILPVPQAVFWLVVPKLIREEKVKLIMTILLLIFLFQFLPKIYHCICLMRRMQKVTGYIFGTIWWGFALNLIAYFIASHVAGGCWYVLAIQRVASCIRQQCMRTGNCNLSLACKEEVCYQFVSPTSTVGYPCLSGNLTSVVNKPMCLDSNGPFRYGIYRWALPVISSNSLAVKILYPIFWGLMTLSTFANDLEPTSNWLEVIFSIVMVLSGLLLFTLLIGNIQVFLHAVMAKKRKMQIRCRDMEWWMKRRQLPSRLRQRVRRFERQRWNALGGEDELELIHDLPPGLRRDIKRYLCFDLINKVPLFRGMDDLILDNICDRAKPRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGGYLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQMAWRRRRKRTRGENIGGSMSPVSENSIEGNSERRLLQYAAMFMSIRPHDHLE

Target ORF information:

RefSeq Version	NM_121545.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Cyclic nucleotide-regulated ion channel family protein (DND1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121545.5

ORF Insert Sequence:

ATGCCCTCTC ACCCCAACTT CATCTTCAGG TGGATTGGAC TGTTTTCCGA TAAGTTCCGT 
CGACAAACGA CTGGGATCGA TGAAAACAGT AACCTCCAAA TCAACGGTGG AGATTCGAGC 
AGCAGCGGCA GCGATGAGAC GCCGGTGCTA AGCTCCGTCG AGTGTTACGC TTGCACACAA 
GTAGGCGTCC CAGCTTTCCA TTCAACTAGC TGCGATCAAG CTCACGCGCC GGAGTGGCGT 
GCCTCCGCCG GCTCTTCTCT AGTTCCGATC CAGGAAGGAT CTGTCCCTAA CCCAGCCCGA 
ACCAGATTCC GACGTCTCAA AGGTCCGTTT GGTGAAGTTC TCGATCCTAG GAGCAAGCGC 
GTGCAGAGAT GGAACCGCGC GTTGCTTTTA GCTCGTGGGA TGGCTTTAGC GGTGGATCCG 
CTCTTCTTCT ACGCGCTTTC CATCGGCCGA ACTACCGGAC CGGCGTGTCT TTACATGGAT 
GGTGCGTTCG CCGCGGTGGT CACGGTGCTC CGCACGTGTC TCGATGCTGT TCATCTTTGG 
CACGTGTGGC TTCAATTCAG ACTGGCCTAC GTCTCGAGAG AGTCGCTTGT CGTTGGTTGT 
GGGAAGCTCG TTTGGGATCC ACGCGCCATC GCGTCTCACT ACGCACGCTC TCTCACTGGC 
TTCTGGTTTG ATGTTATCGT CATCCTCCCT GTCCCTCAGG CAGTGTTTTG GTTAGTTGTG 
CCGAAACTGA TAAGAGAAGA GAAGGTTAAG CTGATAATGA CGATTCTGCT GCTAATATTC 
TTGTTCCAGT TCCTCCCCAA GATTTATCAC TGCATCTGTT TGATGAGAAG GATGCAGAAG 
GTCACTGGTT ACATTTTTGG AACTATTTGG TGGGGTTTTG CTCTTAATCT CATCGCATAT 
TTCATCGCTT CTCATGTTGC TGGGGGATGT TGGTATGTTC TCGCAATACA GCGTGTTGCT 
TCTTGCATAA GACAACAATG TATGAGAACC GGGAACTGCA ATCTGAGTCT GGCTTGCAAA 
GAAGAGGTCT GTTACCAATT TGTGTCACCG ACAAGCACAG TTGGATATCC ATGCTTATCT 
GGAAACCTTA CCAGTGTGGT CAATAAGCCT ATGTGCTTAG ACTCTAACGG ACCATTCCGA 
TATGGTATCT ACCGTTGGGC ACTTCCAGTC ATCTCCAGCA ACTCTCTTGC GGTTAAGATC 
CTTTACCCCA TCTTCTGGGG CCTAATGACT CTCAGCACAT TTGCGAATGA TCTTGAGCCC 
ACAAGCAACT GGCTCGAGGT TATTTTCAGT ATAGTTATGG TTCTAAGTGG CTTGTTACTT 
TTCACGCTGT TGATAGGAAA CATTCAGGTG TTTTTGCATG CGGTAATGGC GAAAAAAAGG 
AAAATGCAGA TACGGTGTAG GGATATGGAA TGGTGGATGA AACGTAGGCA GTTACCTTCC 
CGGTTAAGAC AGAGGGTTAG GCGATTTGAG CGGCAGAGAT GGAATGCCTT GGGTGGTGAA 
GACGAGCTAG AACTTATACA TGATTTGCCT CCGGGTCTTC GAAGAGATAT CAAACGATAT 
CTTTGCTTTG ATCTCATTAA CAAGGTGCCA TTGTTCAGGG GCATGGACGA CTTGATCCTC 
GACAACATTT GCGATCGGGC TAAGCCTCGA GTCTTCTCTA AAGACGAAAA GATCATCCGT 
GAAGGAGATC CTGTACAGAG AATGATATTC ATCATGCGTG GACGAGTCAA ACGTATACAG 
AGCCTAAGCA AAGGCGTCCT AGCCACTAGT ACACTAGAAC CAGGCGGTTA CTTGGGCGAC 
GAGCTACTCT CATGGTGCCT ACGTCGCCCG TTTCTGGACC GTCTTCCCCC TTCCTCAGCA 
ACATTTGTCT GCCTAGAAAA CATCGAGGCA TTCTCCCTCG GATCCGAAGA TCTTAGGTAC 
ATTACCGATC ATTTCCGTTA TAAATTCGCG AACGAGCGGC TTAAGCGGAC CGCAAGATAC 
TATTCCTCAA ACTGGAGGAC GTGGGCAGCG GTAAATATTC AGATGGCGTG GCGCCGGCGT 
AGGAAAAGAA CCCGTGGTGA AAACATCGGC GGTTCGATGA GTCCTGTGTC GGAGAATAGC 
ATTGAAGGTA ACAGTGAACG CCGGTTACTT CAGTATGCAG CTATGTTCAT GTCCATTCGA 
CCGCATGATC ATCTCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DND1 ( NM_203054.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_974783.2 Arabidopsis thaliana Cyclic nucleotide-regulated ion channel family protein (DND1), mRNA.

Price & Availability↑

CloneID	OAb18482
Clone ID Related Accession (Same CDS sequence)	NM_203054.2
Accession Version	NM_203054.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Cyclic nucleotide-regulated ion channel family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On May 26, 2011 this sequence version replaced NM_203054.1.

ATGCAATTCC TTATAATCTC GCTTGAGTGG ATTGGACTGT TTTCCGATAA GTTCCGTCGA 
CAAACGACTG GGATCGATGA AAACAGTAAC CTCCAAATCA ACGGTGGAGA TTCGAGCAGC 
AGCGGCAGCG ATGAGACGCC GGTGCTAAGC TCCGTCGAGT GTTACGCTTG CACACAAGTA 
GGCGTCCCAG CTTTCCATTC AACTAGCTGC GATCAAGCTC ACGCGCCGGA GTGGCGTGCC 
TCCGCCGGCT CTTCTCTAGT TCCGATCCAG GAAGGATCTG TCCCTAACCC AGCCCGAACC 
AGATTCCGAC GTCTCAAAGG TCCGTTTGGT GAAGTTCTCG ATCCTAGGAG CAAGCGCGTG 
CAGAGATGGA ACCGCGCGTT GCTTTTAGCT CGTGGGATGG CTTTAGCGGT GGATCCGCTC 
TTCTTCTACG CGCTTTCCAT CGGCCGAACT ACCGGACCGG CGTGTCTTTA CATGGATGGT 
GCGTTCGCCG CGGTGGTCAC GGTGCTCCGC ACGTGTCTCG ATGCTGTTCA TCTTTGGCAC 
GTGTGGCTTC AATTCAGACT GGCCTACGTC TCGAGAGAGT CGCTTGTCGT TGGTTGTGGG 
AAGCTCGTTT GGGATCCACG CGCCATCGCG TCTCACTACG CACGCTCTCT CACTGGCTTC 
TGGTTTGATG TTATCGTCAT CCTCCCTGTC CCTCAGGCAG TGTTTTGGTT AGTTGTGCCG 
AAACTGATAA GAGAAGAGAA GGTTAAGCTG ATAATGACGA TTCTGCTGCT AATATTCTTG 
TTCCAGTTCC TCCCCAAGAT TTATCACTGC ATCTGTTTGA TGAGAAGGAT GCAGAAGGTC 
ACTGGTTACA TTTTTGGAAC TATTTGGTGG GGTTTTGCTC TTAATCTCAT CGCATATTTC 
ATCGCTTCTC ATGTTGCTGG GGGATGTTGG TATGTTCTCG CAATACAGCG TGTTGCTTCT 
TGCATAAGAC AACAATGTAT GAGAACCGGG AACTGCAATC TGAGTCTGGC TTGCAAAGAA 
GAGGTCTGTT ACCAATTTGT GTCACCGACA AGCACAGTTG GATATCCATG CTTATCTGGA 
AACCTTACCA GTGTGGTCAA TAAGCCTATG TGCTTAGACT CTAACGGACC ATTCCGATAT 
GGTATCTACC GTTGGGCACT TCCAGTCATC TCCAGCAACT CTCTTGCGGT TAAGATCCTT 
TACCCCATCT TCTGGGGCCT AATGACTCTC AGCACATTTG CGAATGATCT TGAGCCCACA 
AGCAACTGGC TCGAGGTTAT TTTCAGTATA GTTATGGTTC TAAGTGGCTT GTTACTTTTC 
ACGCTGTTGA TAGGAAACAT TCAGGTGTTT TTGCATGCGG TAATGGCGAA AAAAAGGAAA 
ATGCAGATAC GGTGTAGGGA TATGGAATGG TGGATGAAAC GTAGGCAGTT ACCTTCCCGG 
TTAAGACAGA GGGTTAGGCG ATTTGAGCGG CAGAGATGGA ATGCCTTGGG TGGTGAAGAC 
GAGCTAGAAC TTATACATGA TTTGCCTCCG GGTCTTCGAA GAGATATCAA ACGATATCTT 
TGCTTTGATC TCATTAACAA GGTGCCATTG TTCAGGGGCA TGGACGACTT GATCCTCGAC 
AACATTTGCG ATCGGGCTAA GCCTCGAGTC TTCTCTAAAG ACGAAAAGAT CATCCGTGAA 
GGAGATCCTG TACAGAGAAT GATATTCATC ATGCGTGGAC GAGTCAAACG TATACAGAGC 
CTAAGCAAAG GCGTCCTAGC CACTAGTACA CTAGAACCAG GCGGTTACTT GGGCGACGAG 
CTACTCTCAT GGTGCCTACG TCGCCCGTTT CTGGACCGTC TTCCCCCTTC CTCAGCAACA 
TTTGTCTGCC TAGAAAACAT CGAGGCATTC TCCCTCGGAT CCGAAGATCT TAGGTACATT 
ACCGATCATT TCCGTTATAA ATTCGCGAAC GAGCGGCTTA AGCGGACCGC AAGATACTAT 
TCCTCAAACT GGAGGACGTG GGCAGCGGTA AATATTCAGA TGGCGTGGCG CCGGCGTAGG 
AAAAGAACCC GTGGTGAAAA CATCGGCGGT TCGATGAGTC CTGTGTCGGA GAATAGCATT 
GAAGGTAACA GTGAACGCCG GTTACTTCAG TATGCAGCTA TGTTCATGTC CATTCGACCG 
CATGATCATC TCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_974783.2
CDS	250..2427
Translation	MQFLIISLEWIGLFSDKFRRQTTGIDENSNLQINGGDSSSSGSDETPVLSSVECYACTQVGVPAFHSTSCDQAHAPEWRASAGSSLVPIQEGSVPNPARTRFRRLKGPFGEVLDPRSKRVQRWNRALLLARGMALAVDPLFFYALSIGRTTGPACLYMDGAFAAVVTVLRTCLDAVHLWHVWLQFRLAYVSRESLVVGCGKLVWDPRAIASHYARSLTGFWFDVIVILPVPQAVFWLVVPKLIREEKVKLIMTILLLIFLFQFLPKIYHCICLMRRMQKVTGYIFGTIWWGFALNLIAYFIASHVAGGCWYVLAIQRVASCIRQQCMRTGNCNLSLACKEEVCYQFVSPTSTVGYPCLSGNLTSVVNKPMCLDSNGPFRYGIYRWALPVISSNSLAVKILYPIFWGLMTLSTFANDLEPTSNWLEVIFSIVMVLSGLLLFTLLIGNIQVFLHAVMAKKRKMQIRCRDMEWWMKRRQLPSRLRQRVRRFERQRWNALGGEDELELIHDLPPGLRRDIKRYLCFDLINKVPLFRGMDDLILDNICDRAKPRVFSKDEKIIREGDPVQRMIFIMRGRVKRIQSLSKGVLATSTLEPGGYLGDELLSWCLRRPFLDRLPPSSATFVCLENIEAFSLGSEDLRYITDHFRYKFANERLKRTARYYSSNWRTWAAVNIQMAWRRRRKRTRGENIGGSMSPVSENSIEGNSERRLLQYAAMFMSIRPHDHLE

Target ORF information:

RefSeq Version	NM_203054.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Cyclic nucleotide-regulated ion channel family protein (DND1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203054.2

ORF Insert Sequence:

ATGCAATTCC TTATAATCTC GCTTGAGTGG ATTGGACTGT TTTCCGATAA GTTCCGTCGA 
CAAACGACTG GGATCGATGA AAACAGTAAC CTCCAAATCA ACGGTGGAGA TTCGAGCAGC 
AGCGGCAGCG ATGAGACGCC GGTGCTAAGC TCCGTCGAGT GTTACGCTTG CACACAAGTA 
GGCGTCCCAG CTTTCCATTC AACTAGCTGC GATCAAGCTC ACGCGCCGGA GTGGCGTGCC 
TCCGCCGGCT CTTCTCTAGT TCCGATCCAG GAAGGATCTG TCCCTAACCC AGCCCGAACC 
AGATTCCGAC GTCTCAAAGG TCCGTTTGGT GAAGTTCTCG ATCCTAGGAG CAAGCGCGTG 
CAGAGATGGA ACCGCGCGTT GCTTTTAGCT CGTGGGATGG CTTTAGCGGT GGATCCGCTC 
TTCTTCTACG CGCTTTCCAT CGGCCGAACT ACCGGACCGG CGTGTCTTTA CATGGATGGT 
GCGTTCGCCG CGGTGGTCAC GGTGCTCCGC ACGTGTCTCG ATGCTGTTCA TCTTTGGCAC 
GTGTGGCTTC AATTCAGACT GGCCTACGTC TCGAGAGAGT CGCTTGTCGT TGGTTGTGGG 
AAGCTCGTTT GGGATCCACG CGCCATCGCG TCTCACTACG CACGCTCTCT CACTGGCTTC 
TGGTTTGATG TTATCGTCAT CCTCCCTGTC CCTCAGGCAG TGTTTTGGTT AGTTGTGCCG 
AAACTGATAA GAGAAGAGAA GGTTAAGCTG ATAATGACGA TTCTGCTGCT AATATTCTTG 
TTCCAGTTCC TCCCCAAGAT TTATCACTGC ATCTGTTTGA TGAGAAGGAT GCAGAAGGTC 
ACTGGTTACA TTTTTGGAAC TATTTGGTGG GGTTTTGCTC TTAATCTCAT CGCATATTTC 
ATCGCTTCTC ATGTTGCTGG GGGATGTTGG TATGTTCTCG CAATACAGCG TGTTGCTTCT 
TGCATAAGAC AACAATGTAT GAGAACCGGG AACTGCAATC TGAGTCTGGC TTGCAAAGAA 
GAGGTCTGTT ACCAATTTGT GTCACCGACA AGCACAGTTG GATATCCATG CTTATCTGGA 
AACCTTACCA GTGTGGTCAA TAAGCCTATG TGCTTAGACT CTAACGGACC ATTCCGATAT 
GGTATCTACC GTTGGGCACT TCCAGTCATC TCCAGCAACT CTCTTGCGGT TAAGATCCTT 
TACCCCATCT TCTGGGGCCT AATGACTCTC AGCACATTTG CGAATGATCT TGAGCCCACA 
AGCAACTGGC TCGAGGTTAT TTTCAGTATA GTTATGGTTC TAAGTGGCTT GTTACTTTTC 
ACGCTGTTGA TAGGAAACAT TCAGGTGTTT TTGCATGCGG TAATGGCGAA AAAAAGGAAA 
ATGCAGATAC GGTGTAGGGA TATGGAATGG TGGATGAAAC GTAGGCAGTT ACCTTCCCGG 
TTAAGACAGA GGGTTAGGCG ATTTGAGCGG CAGAGATGGA ATGCCTTGGG TGGTGAAGAC 
GAGCTAGAAC TTATACATGA TTTGCCTCCG GGTCTTCGAA GAGATATCAA ACGATATCTT 
TGCTTTGATC TCATTAACAA GGTGCCATTG TTCAGGGGCA TGGACGACTT GATCCTCGAC 
AACATTTGCG ATCGGGCTAA GCCTCGAGTC TTCTCTAAAG ACGAAAAGAT CATCCGTGAA 
GGAGATCCTG TACAGAGAAT GATATTCATC ATGCGTGGAC GAGTCAAACG TATACAGAGC 
CTAAGCAAAG GCGTCCTAGC CACTAGTACA CTAGAACCAG GCGGTTACTT GGGCGACGAG 
CTACTCTCAT GGTGCCTACG TCGCCCGTTT CTGGACCGTC TTCCCCCTTC CTCAGCAACA 
TTTGTCTGCC TAGAAAACAT CGAGGCATTC TCCCTCGGAT CCGAAGATCT TAGGTACATT 
ACCGATCATT TCCGTTATAA ATTCGCGAAC GAGCGGCTTA AGCGGACCGC AAGATACTAT 
TCCTCAAACT GGAGGACGTG GGCAGCGGTA AATATTCAGA TGGCGTGGCG CCGGCGTAGG 
AAAAGAACCC GTGGTGAAAA CATCGGCGGT TCGATGAGTC CTGTGTCGGA GAATAGCATT 
GAAGGTAACA GTGAACGCCG GTTACTTCAG TATGCAGCTA TGTTCATGTC CATTCGACCG 
CATGATCATC TCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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DND1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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