NEF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEF1
Entrez Gene ID	831180
Full Name	no exine formation 1
Synonyms	NO EXINE FORMATION 1,T22N19.40,T22N19_40
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_121342.4	NP_196843.1	no exine formation 1
NM_001343271.1	NP_001331232.1	no exine formation 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb40831	NM_001343271.1 Latest version!	Arabidopsis thaliana no exine formation 1 (NEF1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAb32344	NM_121342.4 Latest version!	Arabidopsis thaliana no exine formation 1 (NEF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEF1 ( NM_001343271.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001331232.1 Arabidopsis thaliana no exine formation 1 (NEF1), partial mRNA.

Price & Availability↑

CloneID	OAb40831
Clone ID Related Accession (Same CDS sequence)	NM_001343271.1
Accession Version	NM_001343271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	no exine formation 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). COMPLETENESS: incomplete on the 3' end.

ATGATGCCAC CGGAGCTACA GCCGCGGCTG TTCCGGCCCC ATATAACCGC CTCCACCAGT 
GAACCTACTC AATCTTCCTC CTCTTACTCA CCTCACATGA GCCCTGCATC AACCAGAAAC 
TTCATCGATC GAGCTACTCC TACTTCGAGA TCCAACAACT CTCGATTCTC TCCATCTTCC 
TTCGCCTACA ATGGACGGAT CGCAATTGCT CTTGTCCCTT GCGCCGCCTT CCTCCTCGAC 
CTCGGCGGCA CTCCGGTTGT CGCTACACTC ACAATCGGCC TCCTCATATC TTACATCGTC 
GATTCTCTCA ATGTCAAATT CGGAGGCTTC CTCGGTATAT GGATGTCACT GCTCGCTGCT 
CAGATCTCTT TCTTCTTCTC ATCTTCTCTC TTTTCCTCCT TCAATTCCGT TCCTCTCGGC 
TTGCTCGCGG CTTTTCTCTG CGCTCAGACT ACTTTCCTCA TCGGCTGCTG GACTTCGCTC 
CAATTCAAAT GGCTTCAGCT GGAGAATCCT TCCATTGTTG TCGCTTTGGA GCGTCTCTTG 
TTCGCATGTG TCCCTTTCAC TGCCTCTTCT TTTTTCGCAT GGGCAACTAT CTCTGCCGTT 
GGGATGAACA ACTCTTCTTA CTATTTCCTC TTGTTCGCCT GCGTCTTCTA CTGGATTTTC 
GCTATTCCTA GAGTTTCTTC TTTCAAGACC AAGCAGGAAG TTAAGTACCA TGGAGGAGAG 
ATCCCTGATG ATAGCTTTAT CCTTGGACAG CTGGAGAGCT GTTTCCTTTC ACTGAATTTG 
ATGTTTATGC CGCTTCTCTT TCACGTGGCG TCACATTACT CTGTGATATT CTCATCTGCT 
GCTTCAGTTT GTGATTTGCT GCTCCTGTTC TTTATCCCTT TCCTGTTCCA GCTCTATGCT 
TCGACAAGAG GTGGTCTATG GTGGGTTACC AAGGATTCAC ATCAGCTGCA GAGCATTCGG 
ATTGTGAATG GGGCTATTGC CATGGTCATT ATTGTGATCT GTTTGGAGAT TAGAGTTGTT 
TTCCGCTCTT TTGGAAAGTA TATTCAAGTG CCGCCTCCAT TGAACTACCT TCTGGTGACT 
ACAACATTGC TTGGAGGTGC TGCTGGTGCT GGTGCTTCTG TTCTTGGAAT GATCTCGAGT 
GCTCTGAGCT CAGCCTTTTT CACAGCTTTG TCTGTGATTG TGAGTTCTGC TGGAGCTATC 
GTTGTAGGAT TTCCAGTTCT GTTTACTCCG CTTCCTGCAG TTGCTGGGCT TTATTTTGCT 
CGGTTTTTTA CAAAGAAAAG TGTGCCCTCT TACTTTGCAT TTGTAGCTCT TGGAAGCTTA 
ATGGTTATAT GGTTTGTAAT GCATAATTAC TGGGATTTAA ACATATGGTT AGCCGGCATG 
TTCCTCAAAT CATTCTGTAA GCTTATAGTG GCCAACATTA TTATAGCCAT GGTGATCCCG 
GGTCTTGTTC TTCTCCCTTC AAAATTTCAC TTCCTGACTG AAGCTGGTAT GGTGACCCAC 
GCATTATTAC TGTGTTACAT CGAGGACCGC TTTTTCAATT ACTCAAGCAT ATACTATTAT 
GGAATGGAAG ACGATGTGAT GTACCCTAGC TACATGGTTA TCTTGACGTC GCTAATAGGA 
TTGGCTGTGG TGAGAAGACT GTTTGCTGAC CACCGAATTG GACAAAAGGC AGTCTGGATC 
TTGACCTGTC TGTATTCAGC AAAGTTGGCT ATGCTCTTCC TATCATCGAA ATCAATTGTA 
TGGGTGTCTG CAGCTCTATT ACTGGCTGTT TCCCCTCCCT TACTTCTATA CAAAATGTGC 
AGGGAGAAGT CGAAATCTGC CTCTAAAATG AAGCCATGGC AAGGTTATGC TCATGCCGTA 
GTGGTTGCTG TCTCTGTCTG GTTTTGCCGG GAAACAATAT TTGATGCTCT ACAGTGGTGG 
CATGGAAGAC CTCCGTCTGA TGGTTTGCTT CTTGGTTCCT GCATCGTTTT AATTGGTCTG 
GCCTGTATAC CAATTGTTGC CTTCCACTTT TCGCATGTCC TGTCAGCTAA GAGAAGCTTG 
GTATTAGTAG TGGCAACTGG ATGCATGTTT ATTCTGATGC AGCCACCGAT GCCTATGACG 
TGGAGTTACC ATTCAGATAT GATCAAGGCC GCTCGCCAGT CAGCTGATGA CATTTCCATC 
TATGGATTCA TGGCATCCAA ACCGACTTGG CCGTCTTGGT TGCTTATTGT GTCACTCCTC 
TTGATTCTTG CTGCTGCCAC ATCTCTTATC CCAATCAAAT ACGTAGTAGA ACTGAGAGCA 
TTTTACTCCA TTGCCATGGG TTTAGCTCTC GGTGTTTATA TATCAGCAGA GTTCTTTCTA 
CAAGCTGCTG TTCTACATGC CCTGATCGTT GTAACACTGG TGTGTGCCTC AGTTTTTGTC 
ATCTTCACCC ATTTTCCATC TGCCTCAAGC ACAAAGCTGC TTCCCTGGGT GTTTGCTCTT 
CTCGTGGCTC TATTTCCTGT GACATATTTG TTAGAAGGGC AAGTCAGAAT TAAAAACGAT 
CTCAACGAAA ATGTTACCTG GGGTTGGGAC ACAAGGGAGG AGGATAAGAA AGTCACAACC 
ATGTTGGCCA TTGAAGGTGC AAGAACATCT CTTCTAGGTC TCTATGCAGC GATATTTATG 
CTTATAGCTC TCCTGATTAA ATTTGAGCTC ACTTCGCTTT TGCGTGAGAA ATTCTCTGAG 
AGGAGTGGTC AGTCAAAAAC CCAAGGAGGC GCAAGAGGGA TTTTCCCGAC ACGAATGAGG 
TTGATGCAGC AACGCCGAGC CACCTCGATC CAAAGCTTTG CAGTAGAGAA AATGTCAGAA 
GAGGGAGCAG CATGGATGCC TTCTGTTGGT AATGTCGCCA CCATCATGTG CTTTGCCATA 
TGCTTAATCC TCAACATCCA CCTCTCAGGC GGCTCAAGCC AAGCGATATT CTTCCTGGCT 
CCAATCTTAC TTCTCCTGAA CCAAGACTCT GATCTCTTAT CCGGGTTTGG TGATAAACAG 
AGATACTTCC CGGTTACAGT AGCCATATCA ACATATTTGG CATTATCCTC GCTCTACACT 
GTATGGGAAG AAGTTTGGTT TGGTGGAAAC ACAGGTTGGG GAGTCGAAAT TGGAGGCCGT 
GAATGGTTTT TTGCGGTGAA GAACCTGGCT CTCCTAATAC TCACAGCACC AGGTCACATC 
ATATTCAACA GGTACGTTTG GAGTTACACC ACCAAACACA CAGACGCGTC GCCGATGCTG 
ACCGTGCCAC TTAGCTTTGC AGCCGTAATA ATAACAGACG TGTTTCAAGT TAGGGTTCTG 
GGAGTTTTAG GCATTGTTTA CTCTGCGGCT CAGTATGTGA TATCTAGGCA ACAATATATG 
AAAGGGCTGA GGTATATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001331232.1
CDS	88..3468
Translation	MMPPELQPRLFRPHITASTSEPTQSSSSYSPHMSPASTRNFIDRATPTSRSNNSRFSPSSFAYNGRIAIALVPCAAFLLDLGGTPVVATLTIGLLISYIVDSLNVKFGGFLGIWMSLLAAQISFFFSSSLFSSFNSVPLGLLAAFLCAQTTFLIGCWTSLQFKWLQLENPSIVVALERLLFACVPFTASSFFAWATISAVGMNNSSYYFLLFACVFYWIFAIPRVSSFKTKQEVKYHGGEIPDDSFILGQLESCFLSLNLMFMPLLFHVASHYSVIFSSAASVCDLLLLFFIPFLFQLYASTRGGLWWVTKDSHQLQSIRIVNGAIAMVIIVICLEIRVVFRSFGKYIQVPPPLNYLLVTTTLLGGAAGAGASVLGMISSALSSAFFTALSVIVSSAGAIVVGFPVLFTPLPAVAGLYFARFFTKKSVPSYFAFVALGSLMVIWFVMHNYWDLNIWLAGMFLKSFCKLIVANIIIAMVIPGLVLLPSKFHFLTEAGMVTHALLLCYIEDRFFNYSSIYYYGMEDDVMYPSYMVILTSLIGLAVVRRLFADHRIGQKAVWILTCLYSAKLAMLFLSSKSIVWVSAALLLAVSPPLLLYKMCREKSKSASKMKPWQGYAHAVVVAVSVWFCRETIFDALQWWHGRPPSDGLLLGSCIVLIGLACIPIVAFHFSHVLSAKRSLVLVVATGCMFILMQPPMPMTWSYHSDMIKAARQSADDISIYGFMASKPTWPSWLLIVSLLLILAAATSLIPIKYVVELRAFYSIAMGLALGVYISAEFFLQAAVLHALIVVTLVCASVFVIFTHFPSASSTKLLPWVFALLVALFPVTYLLEGQVRIKNDLNENVTWGWDTREEDKKVTTMLAIEGARTSLLGLYAAIFMLIALLIKFELTSLLREKFSERSGQSKTQGGARGIFPTRMRLMQQRRATSIQSFAVEKMSEEGAAWMPSVGNVATIMCFAICLILNIHLSGGSSQAIFFLAPILLLLNQDSDLLSGFGDKQRYFPVTVAISTYLALSSLYTVWEEVWFGGNTGWGVEIGGREWFFAVKNLALLILTAPGHIIFNRYVWSYTTKHTDASPMLTVPLSFAAVIITDVFQVRVLGVLGIVYSAAQYVISRQQYMKGLRYI

Target ORF information:

RefSeq Version	NM_001343271.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana no exine formation 1 (NEF1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343271.1

ORF Insert Sequence:

ATGATGCCAC CGGAGCTACA GCCGCGGCTG TTCCGGCCCC ATATAACCGC CTCCACCAGT 
GAACCTACTC AATCTTCCTC CTCTTACTCA CCTCACATGA GCCCTGCATC AACCAGAAAC 
TTCATCGATC GAGCTACTCC TACTTCGAGA TCCAACAACT CTCGATTCTC TCCATCTTCC 
TTCGCCTACA ATGGACGGAT CGCAATTGCT CTTGTCCCTT GCGCCGCCTT CCTCCTCGAC 
CTCGGCGGCA CTCCGGTTGT CGCTACACTC ACAATCGGCC TCCTCATATC TTACATCGTC 
GATTCTCTCA ATGTCAAATT CGGAGGCTTC CTCGGTATAT GGATGTCACT GCTCGCTGCT 
CAGATCTCTT TCTTCTTCTC ATCTTCTCTC TTTTCCTCCT TCAATTCCGT TCCTCTCGGC 
TTGCTCGCGG CTTTTCTCTG CGCTCAGACT ACTTTCCTCA TCGGCTGCTG GACTTCGCTC 
CAATTCAAAT GGCTTCAGCT GGAGAATCCT TCCATTGTTG TCGCTTTGGA GCGTCTCTTG 
TTCGCATGTG TCCCTTTCAC TGCCTCTTCT TTTTTCGCAT GGGCAACTAT CTCTGCCGTT 
GGGATGAACA ACTCTTCTTA CTATTTCCTC TTGTTCGCCT GCGTCTTCTA CTGGATTTTC 
GCTATTCCTA GAGTTTCTTC TTTCAAGACC AAGCAGGAAG TTAAGTACCA TGGAGGAGAG 
ATCCCTGATG ATAGCTTTAT CCTTGGACAG CTGGAGAGCT GTTTCCTTTC ACTGAATTTG 
ATGTTTATGC CGCTTCTCTT TCACGTGGCG TCACATTACT CTGTGATATT CTCATCTGCT 
GCTTCAGTTT GTGATTTGCT GCTCCTGTTC TTTATCCCTT TCCTGTTCCA GCTCTATGCT 
TCGACAAGAG GTGGTCTATG GTGGGTTACC AAGGATTCAC ATCAGCTGCA GAGCATTCGG 
ATTGTGAATG GGGCTATTGC CATGGTCATT ATTGTGATCT GTTTGGAGAT TAGAGTTGTT 
TTCCGCTCTT TTGGAAAGTA TATTCAAGTG CCGCCTCCAT TGAACTACCT TCTGGTGACT 
ACAACATTGC TTGGAGGTGC TGCTGGTGCT GGTGCTTCTG TTCTTGGAAT GATCTCGAGT 
GCTCTGAGCT CAGCCTTTTT CACAGCTTTG TCTGTGATTG TGAGTTCTGC TGGAGCTATC 
GTTGTAGGAT TTCCAGTTCT GTTTACTCCG CTTCCTGCAG TTGCTGGGCT TTATTTTGCT 
CGGTTTTTTA CAAAGAAAAG TGTGCCCTCT TACTTTGCAT TTGTAGCTCT TGGAAGCTTA 
ATGGTTATAT GGTTTGTAAT GCATAATTAC TGGGATTTAA ACATATGGTT AGCCGGCATG 
TTCCTCAAAT CATTCTGTAA GCTTATAGTG GCCAACATTA TTATAGCCAT GGTGATCCCG 
GGTCTTGTTC TTCTCCCTTC AAAATTTCAC TTCCTGACTG AAGCTGGTAT GGTGACCCAC 
GCATTATTAC TGTGTTACAT CGAGGACCGC TTTTTCAATT ACTCAAGCAT ATACTATTAT 
GGAATGGAAG ACGATGTGAT GTACCCTAGC TACATGGTTA TCTTGACGTC GCTAATAGGA 
TTGGCTGTGG TGAGAAGACT GTTTGCTGAC CACCGAATTG GACAAAAGGC AGTCTGGATC 
TTGACCTGTC TGTATTCAGC AAAGTTGGCT ATGCTCTTCC TATCATCGAA ATCAATTGTA 
TGGGTGTCTG CAGCTCTATT ACTGGCTGTT TCCCCTCCCT TACTTCTATA CAAAATGTGC 
AGGGAGAAGT CGAAATCTGC CTCTAAAATG AAGCCATGGC AAGGTTATGC TCATGCCGTA 
GTGGTTGCTG TCTCTGTCTG GTTTTGCCGG GAAACAATAT TTGATGCTCT ACAGTGGTGG 
CATGGAAGAC CTCCGTCTGA TGGTTTGCTT CTTGGTTCCT GCATCGTTTT AATTGGTCTG 
GCCTGTATAC CAATTGTTGC CTTCCACTTT TCGCATGTCC TGTCAGCTAA GAGAAGCTTG 
GTATTAGTAG TGGCAACTGG ATGCATGTTT ATTCTGATGC AGCCACCGAT GCCTATGACG 
TGGAGTTACC ATTCAGATAT GATCAAGGCC GCTCGCCAGT CAGCTGATGA CATTTCCATC 
TATGGATTCA TGGCATCCAA ACCGACTTGG CCGTCTTGGT TGCTTATTGT GTCACTCCTC 
TTGATTCTTG CTGCTGCCAC ATCTCTTATC CCAATCAAAT ACGTAGTAGA ACTGAGAGCA 
TTTTACTCCA TTGCCATGGG TTTAGCTCTC GGTGTTTATA TATCAGCAGA GTTCTTTCTA 
CAAGCTGCTG TTCTACATGC CCTGATCGTT GTAACACTGG TGTGTGCCTC AGTTTTTGTC 
ATCTTCACCC ATTTTCCATC TGCCTCAAGC ACAAAGCTGC TTCCCTGGGT GTTTGCTCTT 
CTCGTGGCTC TATTTCCTGT GACATATTTG TTAGAAGGGC AAGTCAGAAT TAAAAACGAT 
CTCAACGAAA ATGTTACCTG GGGTTGGGAC ACAAGGGAGG AGGATAAGAA AGTCACAACC 
ATGTTGGCCA TTGAAGGTGC AAGAACATCT CTTCTAGGTC TCTATGCAGC GATATTTATG 
CTTATAGCTC TCCTGATTAA ATTTGAGCTC ACTTCGCTTT TGCGTGAGAA ATTCTCTGAG 
AGGAGTGGTC AGTCAAAAAC CCAAGGAGGC GCAAGAGGGA TTTTCCCGAC ACGAATGAGG 
TTGATGCAGC AACGCCGAGC CACCTCGATC CAAAGCTTTG CAGTAGAGAA AATGTCAGAA 
GAGGGAGCAG CATGGATGCC TTCTGTTGGT AATGTCGCCA CCATCATGTG CTTTGCCATA 
TGCTTAATCC TCAACATCCA CCTCTCAGGC GGCTCAAGCC AAGCGATATT CTTCCTGGCT 
CCAATCTTAC TTCTCCTGAA CCAAGACTCT GATCTCTTAT CCGGGTTTGG TGATAAACAG 
AGATACTTCC CGGTTACAGT AGCCATATCA ACATATTTGG CATTATCCTC GCTCTACACT 
GTATGGGAAG AAGTTTGGTT TGGTGGAAAC ACAGGTTGGG GAGTCGAAAT TGGAGGCCGT 
GAATGGTTTT TTGCGGTGAA GAACCTGGCT CTCCTAATAC TCACAGCACC AGGTCACATC 
ATATTCAACA GGTACGTTTG GAGTTACACC ACCAAACACA CAGACGCGTC GCCGATGCTG 
ACCGTGCCAC TTAGCTTTGC AGCCGTAATA ATAACAGACG TGTTTCAAGT TAGGGTTCTG 
GGAGTTTTAG GCATTGTTTA CTCTGCGGCT CAGTATGTGA TATCTAGGCA ACAATATATG 
AAAGGGCTGA GGTATATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEF1 ( NM_121342.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196843.1 Arabidopsis thaliana no exine formation 1 (NEF1), mRNA.

Price & Availability↑

CloneID	OAb32344
Clone ID Related Accession (Same CDS sequence)	NM_121342.4
Accession Version	NM_121342.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	no exine formation 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121342.3.

ATGATGCCAC CGGAGCTACA GCCGCGGCTG TTCCGGCCCC ATATAACCGC CTCCACCAGT 
GAACCTACTC AATCTTCCTC CTCTTACTCA CCTCACATGA GCCCTGCATC AACCAGAAAC 
TTCATCGATC GAGCTACTCC TACTTCGAGA TCCAACAACT CTCGATTCTC TCCATCTTCC 
TTCGCCTACA ATGGACGGAT CGCAATTGCT CTTGTCCCTT GCGCCGCCTT CCTCCTCGAC 
CTCGGCGGCA CTCCGGTTGT CGCTACACTC ACAATCGGCC TCCTCATATC TTACATCGTC 
GATTCTCTCA ATGTCAAATT CGGAGGCTTC CTCGGTATAT GGATGTCACT GCTCGCTGCT 
CAGATCTCTT TCTTCTTCTC ATCTTCTCTC TTTTCCTCCT TCAATTCCGT TCCTCTCGGC 
TTGCTCGCGG CTTTTCTCTG CGCTCAGACT ACTTTCCTCA TCGGCTGCTG GACTTCGCTC 
CAATTCAAAT GGCTTCAGCT GGAGAATCCT TCCATTGTTG TCGCTTTGGA GCGTCTCTTG 
TTCGCATGTG TCCCTTTCAC TGCCTCTTCT TTTTTCGCAT GGGCAACTAT CTCTGCCGTT 
GGGATGAACA ACTCTTCTTA CTATTTCCTC TTGTTCGCCT GCGTCTTCTA CTGGATTTTC 
GCTATTCCTA GAGTTTCTTC TTTCAAGACC AAGCAGGAAG TTAAGTACCA TGGAGGAGAG 
ATCCCTGATG ATAGCTTTAT CCTTGGACAG CTGGAGAGCT GTTTCCTTTC ACTGAATTTG 
ATGTTTATGC CGCTTCTCTT TCACGTGGCG TCACATTACT CTGTGATATT CTCATCTGCT 
GCTTCAGTTT GTGATTTGCT GCTCCTGTTC TTTATCCCTT TCCTGTTCCA GCTCTATGCT 
TCGACAAGAG GTGGTCTATG GTGGGTTACC AAGGATTCAC ATCAGCTGCA GAGCATTCGG 
ATTGTGAATG GGGCTATTGC CATGGTCATT ATTGTGATCT GTTTGGAGAT TAGAGTTGTT 
TTCCGCTCTT TTGGAAAGTA TATTCAAGTG CCGCCTCCAT TGAACTACCT TCTGGTGACT 
ACAACATTGC TTGGAGGTGC TGCTGGTGCT GGTGCTTCTG TTCTTGGAAT GATCTCGAGT 
GCTCTGAGCT CAGCCTTTTT CACAGCTTTG TCTGTGATTG TGAGTTCTGC TGGAGCTATC 
GTTGTAGGAT TTCCAGTTCT GTTTACTCCG CTTCCTGCAG TTGCTGGGCT TTATTTTGCT 
CGGTTTTTTA CAAAGAAAAG TGTGCCCTCT TACTTTGCAT TTGTAGCTCT TGGAAGCTTA 
ATGGTTATAT GGTTTGTAAT GCATAATTAC TGGGATTTAA ACATATGGTT AGCCGGCATG 
TTCCTCAAAT CATTCTGTAA GCTTATAGTG GCCAACATTA TTATAGCCAT GGTGATCCCG 
GGTCTTGTTC TTCTCCCTTC AAAATTTCAC TTCCTGACTG AAGCTGGTAT GGTGACCCAC 
GCATTATTAC TGTGTTACAT CGAGGACCGC TTTTTCAATT ACTCAAGCAT ATACTATTAT 
GGAATGGAAG ACGATGTGAT GTACCCTAGC TACATGGTTA TCTTGACGTC GCTAATAGGA 
TTGGCTGTGG TGAGAAGACT GTTTGCTGAC CACCGAATTG GACAAAAGGC AGTCTGGATC 
TTGACCTGTC TGTATTCAGC AAAGTTGGCT ATGCTCTTCC TATCATCGAA ATCAATTGTA 
TGGGTGTCTG CAGCTCTATT ACTGGCTGTT TCCCCTCCCT TACTTCTATA CAAGGAGAAG 
TCGAAATCTG CCTCTAAAAT GAAGCCATGG CAAGGTTATG CTCATGCCGT AGTGGTTGCT 
GTCTCTGTCT GGTTTTGCCG GGAAACAATA TTTGATGCTC TACAGTGGTG GCATGGAAGA 
CCTCCGTCTG ATGGTTTGCT TCTTGGTTCC TGCATCGTTT TAATTGGTCT GGCCTGTATA 
CCAATTGTTG CCTTCCACTT TTCGCATGTC CTGTCAGCTA AGAGAAGCTT GGTATTAGTA 
GTGGCAACTG GATGCATGTT TATTCTGATG CAGCCACCGA TGCCTATGAC GTGGAGTTAC 
CATTCAGATA TGATCAAGGC CGCTCGCCAG TCAGCTGATG ACATTTCCAT CTATGGATTC 
ATGGCATCCA AACCGACTTG GCCGTCTTGG TTGCTTATTG TGTCACTCCT CTTGATTCTT 
GCTGCTGCCA CATCTCTTAT CCCAATCAAA TACGTAGTAG AACTGAGAGC ATTTTACTCC 
ATTGCCATGG GTTTAGCTCT CGGTGTTTAT ATATCAGCAG AGTTCTTTCT ACAAGCTGCT 
GTTCTACATG CCCTGATCGT TGTAACACTG GTGTGTGCCT CAGTTTTTGT CATCTTCACC 
CATTTTCCAT CTGCCTCAAG CACAAAGCTG CTTCCCTGGG TGTTTGCTCT TCTCGTGGCT 
CTATTTCCTG TGACATATTT GTTAGAAGGG CAAGTCAGAA TTAAAAACGA TCTCAACGAA 
AATGTTACCT GGGGTTGGGA CACAAGGGAG GAGGATAAGA AAGTCACAAC CATGTTGGCC 
ATTGAAGGTG CAAGAACATC TCTTCTAGGT CTCTATGCAG CGATATTTAT GCTTATAGCT 
CTCCTGATTA AATTTGAGCT CACTTCGCTT TTGCGTGAGA AATTCTCTGA GAGGAGTGGT 
CAGTCAAAAA CCCAAGGAGG CGCAAGAGGG ATTTTCCCGA CACGAATGAG GTTGATGCAG 
CAACGCCGAG CCACCTCGAT CCAAAGCTTT GCAGTAGAGA AAATGTCAGA AGAGGGAGCA 
GCATGGATGC CTTCTGTTGG TAATGTCGCC ACCATCATGT GCTTTGCCAT ATGCTTAATC 
CTCAACATCC ACCTCTCAGG CGGCTCAAGC CAAGCGATAT TCTTCCTGGC TCCAATCTTA 
CTTCTCCTGA ACCAAGACTC TGATCTCTTA TCCGGGTTTG GTGATAAACA GAGATACTTC 
CCGGTTACAG TAGCCATATC AACATATTTG GCATTATCCT CGCTCTACAC TGTATGGGAA 
GAAGTTTGGT TTGGTGGAAA CACAGGTTGG GGAGTCGAAA TTGGAGGCCG TGAATGGTTT 
TTTGCGGTGA AGAACCTGGC TCTCCTAATA CTCACAGCAC CAGGTCACAT CATATTCAAC 
AGGTACGTTT GGAGTTACAC CACCAAACAC ACAGACGCGT CGCCGATGCT GACCGTGCCA 
CTTAGCTTTG CAGCCGTAAT AATAACAGAC GTGTTTCAAG TTAGGGTTCT GGGAGTTTTA 
GGCATTGTTT ACTCTGCGGC TCAGTATGTG ATATCTAGGC AACAATATAT GAAAGGGCTG 
AGGTATATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196843.1
CDS	106..3477
Translation	MMPPELQPRLFRPHITASTSEPTQSSSSYSPHMSPASTRNFIDRATPTSRSNNSRFSPSSFAYNGRIAIALVPCAAFLLDLGGTPVVATLTIGLLISYIVDSLNVKFGGFLGIWMSLLAAQISFFFSSSLFSSFNSVPLGLLAAFLCAQTTFLIGCWTSLQFKWLQLENPSIVVALERLLFACVPFTASSFFAWATISAVGMNNSSYYFLLFACVFYWIFAIPRVSSFKTKQEVKYHGGEIPDDSFILGQLESCFLSLNLMFMPLLFHVASHYSVIFSSAASVCDLLLLFFIPFLFQLYASTRGGLWWVTKDSHQLQSIRIVNGAIAMVIIVICLEIRVVFRSFGKYIQVPPPLNYLLVTTTLLGGAAGAGASVLGMISSALSSAFFTALSVIVSSAGAIVVGFPVLFTPLPAVAGLYFARFFTKKSVPSYFAFVALGSLMVIWFVMHNYWDLNIWLAGMFLKSFCKLIVANIIIAMVIPGLVLLPSKFHFLTEAGMVTHALLLCYIEDRFFNYSSIYYYGMEDDVMYPSYMVILTSLIGLAVVRRLFADHRIGQKAVWILTCLYSAKLAMLFLSSKSIVWVSAALLLAVSPPLLLYKEKSKSASKMKPWQGYAHAVVVAVSVWFCRETIFDALQWWHGRPPSDGLLLGSCIVLIGLACIPIVAFHFSHVLSAKRSLVLVVATGCMFILMQPPMPMTWSYHSDMIKAARQSADDISIYGFMASKPTWPSWLLIVSLLLILAAATSLIPIKYVVELRAFYSIAMGLALGVYISAEFFLQAAVLHALIVVTLVCASVFVIFTHFPSASSTKLLPWVFALLVALFPVTYLLEGQVRIKNDLNENVTWGWDTREEDKKVTTMLAIEGARTSLLGLYAAIFMLIALLIKFELTSLLREKFSERSGQSKTQGGARGIFPTRMRLMQQRRATSIQSFAVEKMSEEGAAWMPSVGNVATIMCFAICLILNIHLSGGSSQAIFFLAPILLLLNQDSDLLSGFGDKQRYFPVTVAISTYLALSSLYTVWEEVWFGGNTGWGVEIGGREWFFAVKNLALLILTAPGHIIFNRYVWSYTTKHTDASPMLTVPLSFAAVIITDVFQVRVLGVLGIVYSAAQYVISRQQYMKGLRYI

Target ORF information:

RefSeq Version	NM_121342.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana no exine formation 1 (NEF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121342.4

ORF Insert Sequence:

ATGATGCCAC CGGAGCTACA GCCGCGGCTG TTCCGGCCCC ATATAACCGC CTCCACCAGT 
GAACCTACTC AATCTTCCTC CTCTTACTCA CCTCACATGA GCCCTGCATC AACCAGAAAC 
TTCATCGATC GAGCTACTCC TACTTCGAGA TCCAACAACT CTCGATTCTC TCCATCTTCC 
TTCGCCTACA ATGGACGGAT CGCAATTGCT CTTGTCCCTT GCGCCGCCTT CCTCCTCGAC 
CTCGGCGGCA CTCCGGTTGT CGCTACACTC ACAATCGGCC TCCTCATATC TTACATCGTC 
GATTCTCTCA ATGTCAAATT CGGAGGCTTC CTCGGTATAT GGATGTCACT GCTCGCTGCT 
CAGATCTCTT TCTTCTTCTC ATCTTCTCTC TTTTCCTCCT TCAATTCCGT TCCTCTCGGC 
TTGCTCGCGG CTTTTCTCTG CGCTCAGACT ACTTTCCTCA TCGGCTGCTG GACTTCGCTC 
CAATTCAAAT GGCTTCAGCT GGAGAATCCT TCCATTGTTG TCGCTTTGGA GCGTCTCTTG 
TTCGCATGTG TCCCTTTCAC TGCCTCTTCT TTTTTCGCAT GGGCAACTAT CTCTGCCGTT 
GGGATGAACA ACTCTTCTTA CTATTTCCTC TTGTTCGCCT GCGTCTTCTA CTGGATTTTC 
GCTATTCCTA GAGTTTCTTC TTTCAAGACC AAGCAGGAAG TTAAGTACCA TGGAGGAGAG 
ATCCCTGATG ATAGCTTTAT CCTTGGACAG CTGGAGAGCT GTTTCCTTTC ACTGAATTTG 
ATGTTTATGC CGCTTCTCTT TCACGTGGCG TCACATTACT CTGTGATATT CTCATCTGCT 
GCTTCAGTTT GTGATTTGCT GCTCCTGTTC TTTATCCCTT TCCTGTTCCA GCTCTATGCT 
TCGACAAGAG GTGGTCTATG GTGGGTTACC AAGGATTCAC ATCAGCTGCA GAGCATTCGG 
ATTGTGAATG GGGCTATTGC CATGGTCATT ATTGTGATCT GTTTGGAGAT TAGAGTTGTT 
TTCCGCTCTT TTGGAAAGTA TATTCAAGTG CCGCCTCCAT TGAACTACCT TCTGGTGACT 
ACAACATTGC TTGGAGGTGC TGCTGGTGCT GGTGCTTCTG TTCTTGGAAT GATCTCGAGT 
GCTCTGAGCT CAGCCTTTTT CACAGCTTTG TCTGTGATTG TGAGTTCTGC TGGAGCTATC 
GTTGTAGGAT TTCCAGTTCT GTTTACTCCG CTTCCTGCAG TTGCTGGGCT TTATTTTGCT 
CGGTTTTTTA CAAAGAAAAG TGTGCCCTCT TACTTTGCAT TTGTAGCTCT TGGAAGCTTA 
ATGGTTATAT GGTTTGTAAT GCATAATTAC TGGGATTTAA ACATATGGTT AGCCGGCATG 
TTCCTCAAAT CATTCTGTAA GCTTATAGTG GCCAACATTA TTATAGCCAT GGTGATCCCG 
GGTCTTGTTC TTCTCCCTTC AAAATTTCAC TTCCTGACTG AAGCTGGTAT GGTGACCCAC 
GCATTATTAC TGTGTTACAT CGAGGACCGC TTTTTCAATT ACTCAAGCAT ATACTATTAT 
GGAATGGAAG ACGATGTGAT GTACCCTAGC TACATGGTTA TCTTGACGTC GCTAATAGGA 
TTGGCTGTGG TGAGAAGACT GTTTGCTGAC CACCGAATTG GACAAAAGGC AGTCTGGATC 
TTGACCTGTC TGTATTCAGC AAAGTTGGCT ATGCTCTTCC TATCATCGAA ATCAATTGTA 
TGGGTGTCTG CAGCTCTATT ACTGGCTGTT TCCCCTCCCT TACTTCTATA CAAGGAGAAG 
TCGAAATCTG CCTCTAAAAT GAAGCCATGG CAAGGTTATG CTCATGCCGT AGTGGTTGCT 
GTCTCTGTCT GGTTTTGCCG GGAAACAATA TTTGATGCTC TACAGTGGTG GCATGGAAGA 
CCTCCGTCTG ATGGTTTGCT TCTTGGTTCC TGCATCGTTT TAATTGGTCT GGCCTGTATA 
CCAATTGTTG CCTTCCACTT TTCGCATGTC CTGTCAGCTA AGAGAAGCTT GGTATTAGTA 
GTGGCAACTG GATGCATGTT TATTCTGATG CAGCCACCGA TGCCTATGAC GTGGAGTTAC 
CATTCAGATA TGATCAAGGC CGCTCGCCAG TCAGCTGATG ACATTTCCAT CTATGGATTC 
ATGGCATCCA AACCGACTTG GCCGTCTTGG TTGCTTATTG TGTCACTCCT CTTGATTCTT 
GCTGCTGCCA CATCTCTTAT CCCAATCAAA TACGTAGTAG AACTGAGAGC ATTTTACTCC 
ATTGCCATGG GTTTAGCTCT CGGTGTTTAT ATATCAGCAG AGTTCTTTCT ACAAGCTGCT 
GTTCTACATG CCCTGATCGT TGTAACACTG GTGTGTGCCT CAGTTTTTGT CATCTTCACC 
CATTTTCCAT CTGCCTCAAG CACAAAGCTG CTTCCCTGGG TGTTTGCTCT TCTCGTGGCT 
CTATTTCCTG TGACATATTT GTTAGAAGGG CAAGTCAGAA TTAAAAACGA TCTCAACGAA 
AATGTTACCT GGGGTTGGGA CACAAGGGAG GAGGATAAGA AAGTCACAAC CATGTTGGCC 
ATTGAAGGTG CAAGAACATC TCTTCTAGGT CTCTATGCAG CGATATTTAT GCTTATAGCT 
CTCCTGATTA AATTTGAGCT CACTTCGCTT TTGCGTGAGA AATTCTCTGA GAGGAGTGGT 
CAGTCAAAAA CCCAAGGAGG CGCAAGAGGG ATTTTCCCGA CACGAATGAG GTTGATGCAG 
CAACGCCGAG CCACCTCGAT CCAAAGCTTT GCAGTAGAGA AAATGTCAGA AGAGGGAGCA 
GCATGGATGC CTTCTGTTGG TAATGTCGCC ACCATCATGT GCTTTGCCAT ATGCTTAATC 
CTCAACATCC ACCTCTCAGG CGGCTCAAGC CAAGCGATAT TCTTCCTGGC TCCAATCTTA 
CTTCTCCTGA ACCAAGACTC TGATCTCTTA TCCGGGTTTG GTGATAAACA GAGATACTTC 
CCGGTTACAG TAGCCATATC AACATATTTG GCATTATCCT CGCTCTACAC TGTATGGGAA 
GAAGTTTGGT TTGGTGGAAA CACAGGTTGG GGAGTCGAAA TTGGAGGCCG TGAATGGTTT 
TTTGCGGTGA AGAACCTGGC TCTCCTAATA CTCACAGCAC CAGGTCACAT CATATTCAAC 
AGGTACGTTT GGAGTTACAC CACCAAACAC ACAGACGCGT CGCCGATGCT GACCGTGCCA 
CTTAGCTTTG CAGCCGTAAT AATAACAGAC GTGTTTCAAG TTAGGGTTCT GGGAGTTTTA 
GGCATTGTTT ACTCTGCGGC TCAGTATGTG ATATCTAGGC AACAATATAT GAAAGGGCTG 
AGGTATATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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NEF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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