D2HGDH cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following D2HGDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the D2HGDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb32052 NM_202963.2
Latest version!
Arabidopsis thaliana FAD-linked oxidases family protein (D2HGDH), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OAb32052 NM_001342409.1
Latest version!
Arabidopsis thaliana FAD-linked oxidases family protein (D2HGDH), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb32052
    Clone ID Related Accession (Same CDS sequence) NM_001342409.1 , NM_202963.2
    Accession Version NM_001342409.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product FAD-linked oxidases family protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATGATGC AGAAATTGAG AAGATCGGGG GAGTTCATAA GATTTGGATG CAAATCTCTA 
    ATTAGTAGCC GACCAAACAA GGACTCAGTG TCACGTTCTG TGTCAGGCTT TGTGAATCAC
    TACAAATCAA AAGGCAAGCT TTTTGAGCTA AGTGATGGGA ACTATAAGAC AGAGCTTCAT
    CATCCATGTA TTAGTAGAAA TGTGGGGATG CTTCTTCAGC AGTATAAGTG TTTTGGATCA
    TCAGCAGCTT CTTTAATTCA GAGAAACCCT TTGTTTTCGT CGTTGGATTC GAAAGATGTT
    AGCTACTTTA AGGAGATATT AGGTGAAAAA AACGTGGTTG AAGATAAAGA AAGGCTTGAG
    ACTGCTAATA CAGATTGGAT GCATAAGTAC AAAGGATCTA GTAAGCTGAT GCTCTTGCCC
    AAGAATACAC AAGAGGTGTC TCAGATACTT GAGTACTGTG ATTCGAGGCG TTTAGCAGTT
    GTTCCTCAAG GAGGAAACAC TGGTCTTGTT GGTGGAAGTG TGCCTGTCTT TGATGAGGTG
    ATCGTCAATG TTGGTCTGAT GAACAAAATC TTATCTTTTG ATGAGGTTAG TGGCGTCTTG
    GTGTGTGAAG CAGGATGCAT ATTAGAAAAT CTGGCAACTT TCCTTGACAC AAAAGGTTTT
    ATTATGCCTC TAGACTTAGG TGCAAAAGGA AGCTGTCATA TCGGTGGAAA TGTTTCAACT
    AATGCTGGTG GTTTGCGTCT AATCCGTTAT GGCTCACTTC ATGGAACTGT ATTGGGTCTA
    GAAGCTGTCA CAGCAAATGG CAACGTGCTT GACATGCTTG GAACTTTACG CAAAGACAAT
    ACTGGGTACG ACTTAAAACA TTTGTTTATT GGTAGTGAAG GATCACTTGG TATTGTAACT
    AAAGTTTCTA TTCTCACACA ACCAAAATTG TCTTCTGTAA ATTTAGCCTT CATTGCTTGC
    AAAGATTATC TCAGCTGCCA GAAACTTCTT GTTGAAGCAA AGAGAAATCT TGGAGAGATA
    CTCTCGGCTT TCGAGTTTCT TGATAACAAT TCCATGGATT TGGTACTGAA CCACCTAGAC
    GGTGTACGTA ATCCAGTTTC CTCTTCGGAG AACTTTTATA TTCTGATCGA GACAACAGGG
    AGTGATGAAA CTAATGACAG GGAGAAGCTT GAAGCTTTCC TGTTGAAGTC ACTGGAAAAA
    GGTTTAGTTT CTGATGGTGT AATCGCTCAA GACATTAACC AGGCATCCTC ATTTTGGCGC
    ATACGAGAGG GTATAACAGA GGCGTTACAG AAAGCAGGAG CTGTTTACAA GTATGACTTA
    TCCTTACCGG TTGAAGAAAT TTACAATATT GTTAACGATC TTCGAGGGAG ATTAGGTGAC
    TTAGCAAATG TTATGGGATA TGGTCACCTT GGAGACGGAA ATCTACATTT AAACATCTCA
    GCCGCGGAAT ATAACGATAA GCTTTTAGGT TTGATAGAGC CTTATGTCTA TGAGTGGACA
    TCAAAGCACC GTGGAAGCAT CAGTGCGGAA CATGGATTAG GTGTAATGAA AGCTAATGAA
    ATCTTCTACA GCAAATCACC CGAAACTGTT GCATTAATGG CTTCCATTAA AAAGTTGCTG
    GACCCAAAGG GAATTCTCAA CCCTTACAAA GTTCTTCCTC ACTCTCTCTT CTCCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001320151.1
    CDS54..1733
    Translation

    Target ORF information:

    RefSeq Version NM_001342409.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana FAD-linked oxidases family protein (D2HGDH), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001342409.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATGATGC AGAAATTGAG AAGATCGGGG GAGTTCATAA GATTTGGATG CAAATCTCTA 
    ATTAGTAGCC GACCAAACAA GGACTCAGTG TCACGTTCTG TGTCAGGCTT TGTGAATCAC
    TACAAATCAA AAGGCAAGCT TTTTGAGCTA AGTGATGGGA ACTATAAGAC AGAGCTTCAT
    CATCCATGTA TTAGTAGAAA TGTGGGGATG CTTCTTCAGC AGTATAAGTG TTTTGGATCA
    TCAGCAGCTT CTTTAATTCA GAGAAACCCT TTGTTTTCGT CGTTGGATTC GAAAGATGTT
    AGCTACTTTA AGGAGATATT AGGTGAAAAA AACGTGGTTG AAGATAAAGA AAGGCTTGAG
    ACTGCTAATA CAGATTGGAT GCATAAGTAC AAAGGATCTA GTAAGCTGAT GCTCTTGCCC
    AAGAATACAC AAGAGGTGTC TCAGATACTT GAGTACTGTG ATTCGAGGCG TTTAGCAGTT
    GTTCCTCAAG GAGGAAACAC TGGTCTTGTT GGTGGAAGTG TGCCTGTCTT TGATGAGGTG
    ATCGTCAATG TTGGTCTGAT GAACAAAATC TTATCTTTTG ATGAGGTTAG TGGCGTCTTG
    GTGTGTGAAG CAGGATGCAT ATTAGAAAAT CTGGCAACTT TCCTTGACAC AAAAGGTTTT
    ATTATGCCTC TAGACTTAGG TGCAAAAGGA AGCTGTCATA TCGGTGGAAA TGTTTCAACT
    AATGCTGGTG GTTTGCGTCT AATCCGTTAT GGCTCACTTC ATGGAACTGT ATTGGGTCTA
    GAAGCTGTCA CAGCAAATGG CAACGTGCTT GACATGCTTG GAACTTTACG CAAAGACAAT
    ACTGGGTACG ACTTAAAACA TTTGTTTATT GGTAGTGAAG GATCACTTGG TATTGTAACT
    AAAGTTTCTA TTCTCACACA ACCAAAATTG TCTTCTGTAA ATTTAGCCTT CATTGCTTGC
    AAAGATTATC TCAGCTGCCA GAAACTTCTT GTTGAAGCAA AGAGAAATCT TGGAGAGATA
    CTCTCGGCTT TCGAGTTTCT TGATAACAAT TCCATGGATT TGGTACTGAA CCACCTAGAC
    GGTGTACGTA ATCCAGTTTC CTCTTCGGAG AACTTTTATA TTCTGATCGA GACAACAGGG
    AGTGATGAAA CTAATGACAG GGAGAAGCTT GAAGCTTTCC TGTTGAAGTC ACTGGAAAAA
    GGTTTAGTTT CTGATGGTGT AATCGCTCAA GACATTAACC AGGCATCCTC ATTTTGGCGC
    ATACGAGAGG GTATAACAGA GGCGTTACAG AAAGCAGGAG CTGTTTACAA GTATGACTTA
    TCCTTACCGG TTGAAGAAAT TTACAATATT GTTAACGATC TTCGAGGGAG ATTAGGTGAC
    TTAGCAAATG TTATGGGATA TGGTCACCTT GGAGACGGAA ATCTACATTT AAACATCTCA
    GCCGCGGAAT ATAACGATAA GCTTTTAGGT TTGATAGAGC CTTATGTCTA TGAGTGGACA
    TCAAAGCACC GTGGAAGCAT CAGTGCGGAA CATGGATTAG GTGTAATGAA AGCTAATGAA
    ATCTTCTACA GCAAATCACC CGAAACTGTT GCATTAATGG CTTCCATTAA AAAGTTGCTG
    GACCCAAAGG GAATTCTCAA CCCTTACAAA GTTCTTCCTC ACTCTCTCTT CTCCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb32052
    Clone ID Related Accession (Same CDS sequence) NM_001342409.1 , NM_202963.2
    Accession Version NM_202963.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product FAD-linked oxidases family protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_202963.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATGATGC AGAAATTGAG AAGATCGGGG GAGTTCATAA GATTTGGATG CAAATCTCTA 
    ATTAGTAGCC GACCAAACAA GGACTCAGTG TCACGTTCTG TGTCAGGCTT TGTGAATCAC
    TACAAATCAA AAGGCAAGCT TTTTGAGCTA AGTGATGGGA ACTATAAGAC AGAGCTTCAT
    CATCCATGTA TTAGTAGAAA TGTGGGGATG CTTCTTCAGC AGTATAAGTG TTTTGGATCA
    TCAGCAGCTT CTTTAATTCA GAGAAACCCT TTGTTTTCGT CGTTGGATTC GAAAGATGTT
    AGCTACTTTA AGGAGATATT AGGTGAAAAA AACGTGGTTG AAGATAAAGA AAGGCTTGAG
    ACTGCTAATA CAGATTGGAT GCATAAGTAC AAAGGATCTA GTAAGCTGAT GCTCTTGCCC
    AAGAATACAC AAGAGGTGTC TCAGATACTT GAGTACTGTG ATTCGAGGCG TTTAGCAGTT
    GTTCCTCAAG GAGGAAACAC TGGTCTTGTT GGTGGAAGTG TGCCTGTCTT TGATGAGGTG
    ATCGTCAATG TTGGTCTGAT GAACAAAATC TTATCTTTTG ATGAGGTTAG TGGCGTCTTG
    GTGTGTGAAG CAGGATGCAT ATTAGAAAAT CTGGCAACTT TCCTTGACAC AAAAGGTTTT
    ATTATGCCTC TAGACTTAGG TGCAAAAGGA AGCTGTCATA TCGGTGGAAA TGTTTCAACT
    AATGCTGGTG GTTTGCGTCT AATCCGTTAT GGCTCACTTC ATGGAACTGT ATTGGGTCTA
    GAAGCTGTCA CAGCAAATGG CAACGTGCTT GACATGCTTG GAACTTTACG CAAAGACAAT
    ACTGGGTACG ACTTAAAACA TTTGTTTATT GGTAGTGAAG GATCACTTGG TATTGTAACT
    AAAGTTTCTA TTCTCACACA ACCAAAATTG TCTTCTGTAA ATTTAGCCTT CATTGCTTGC
    AAAGATTATC TCAGCTGCCA GAAACTTCTT GTTGAAGCAA AGAGAAATCT TGGAGAGATA
    CTCTCGGCTT TCGAGTTTCT TGATAACAAT TCCATGGATT TGGTACTGAA CCACCTAGAC
    GGTGTACGTA ATCCAGTTTC CTCTTCGGAG AACTTTTATA TTCTGATCGA GACAACAGGG
    AGTGATGAAA CTAATGACAG GGAGAAGCTT GAAGCTTTCC TGTTGAAGTC ACTGGAAAAA
    GGTTTAGTTT CTGATGGTGT AATCGCTCAA GACATTAACC AGGCATCCTC ATTTTGGCGC
    ATACGAGAGG GTATAACAGA GGCGTTACAG AAAGCAGGAG CTGTTTACAA GTATGACTTA
    TCCTTACCGG TTGAAGAAAT TTACAATATT GTTAACGATC TTCGAGGGAG ATTAGGTGAC
    TTAGCAAATG TTATGGGATA TGGTCACCTT GGAGACGGAA ATCTACATTT AAACATCTCA
    GCCGCGGAAT ATAACGATAA GCTTTTAGGT TTGATAGAGC CTTATGTCTA TGAGTGGACA
    TCAAAGCACC GTGGAAGCAT CAGTGCGGAA CATGGATTAG GTGTAATGAA AGCTAATGAA
    ATCTTCTACA GCAAATCACC CGAAACTGTT GCATTAATGG CTTCCATTAA AAAGTTGCTG
    GACCCAAAGG GAATTCTCAA CCCTTACAAA GTTCTTCCTC ACTCTCTCTT CTCCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_974692.1
    CDS78..1757
    Translation

    Target ORF information:

    RefSeq Version NM_202963.2
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana FAD-linked oxidases family protein (D2HGDH), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_202963.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATGATGC AGAAATTGAG AAGATCGGGG GAGTTCATAA GATTTGGATG CAAATCTCTA 
    ATTAGTAGCC GACCAAACAA GGACTCAGTG TCACGTTCTG TGTCAGGCTT TGTGAATCAC
    TACAAATCAA AAGGCAAGCT TTTTGAGCTA AGTGATGGGA ACTATAAGAC AGAGCTTCAT
    CATCCATGTA TTAGTAGAAA TGTGGGGATG CTTCTTCAGC AGTATAAGTG TTTTGGATCA
    TCAGCAGCTT CTTTAATTCA GAGAAACCCT TTGTTTTCGT CGTTGGATTC GAAAGATGTT
    AGCTACTTTA AGGAGATATT AGGTGAAAAA AACGTGGTTG AAGATAAAGA AAGGCTTGAG
    ACTGCTAATA CAGATTGGAT GCATAAGTAC AAAGGATCTA GTAAGCTGAT GCTCTTGCCC
    AAGAATACAC AAGAGGTGTC TCAGATACTT GAGTACTGTG ATTCGAGGCG TTTAGCAGTT
    GTTCCTCAAG GAGGAAACAC TGGTCTTGTT GGTGGAAGTG TGCCTGTCTT TGATGAGGTG
    ATCGTCAATG TTGGTCTGAT GAACAAAATC TTATCTTTTG ATGAGGTTAG TGGCGTCTTG
    GTGTGTGAAG CAGGATGCAT ATTAGAAAAT CTGGCAACTT TCCTTGACAC AAAAGGTTTT
    ATTATGCCTC TAGACTTAGG TGCAAAAGGA AGCTGTCATA TCGGTGGAAA TGTTTCAACT
    AATGCTGGTG GTTTGCGTCT AATCCGTTAT GGCTCACTTC ATGGAACTGT ATTGGGTCTA
    GAAGCTGTCA CAGCAAATGG CAACGTGCTT GACATGCTTG GAACTTTACG CAAAGACAAT
    ACTGGGTACG ACTTAAAACA TTTGTTTATT GGTAGTGAAG GATCACTTGG TATTGTAACT
    AAAGTTTCTA TTCTCACACA ACCAAAATTG TCTTCTGTAA ATTTAGCCTT CATTGCTTGC
    AAAGATTATC TCAGCTGCCA GAAACTTCTT GTTGAAGCAA AGAGAAATCT TGGAGAGATA
    CTCTCGGCTT TCGAGTTTCT TGATAACAAT TCCATGGATT TGGTACTGAA CCACCTAGAC
    GGTGTACGTA ATCCAGTTTC CTCTTCGGAG AACTTTTATA TTCTGATCGA GACAACAGGG
    AGTGATGAAA CTAATGACAG GGAGAAGCTT GAAGCTTTCC TGTTGAAGTC ACTGGAAAAA
    GGTTTAGTTT CTGATGGTGT AATCGCTCAA GACATTAACC AGGCATCCTC ATTTTGGCGC
    ATACGAGAGG GTATAACAGA GGCGTTACAG AAAGCAGGAG CTGTTTACAA GTATGACTTA
    TCCTTACCGG TTGAAGAAAT TTACAATATT GTTAACGATC TTCGAGGGAG ATTAGGTGAC
    TTAGCAAATG TTATGGGATA TGGTCACCTT GGAGACGGAA ATCTACATTT AAACATCTCA
    GCCGCGGAAT ATAACGATAA GCTTTTAGGT TTGATAGAGC CTTATGTCTA TGAGTGGACA
    TCAAAGCACC GTGGAAGCAT CAGTGCGGAA CATGGATTAG GTGTAATGAA AGCTAATGAA
    ATCTTCTACA GCAAATCACC CGAAACTGTT GCATTAATGG CTTCCATTAA AAAGTTGCTG
    GACCCAAAGG GAATTCTCAA CCCTTACAAA GTTCTTCCTC ACTCTCTCTT CTCCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
    Nature402(6763)769-77(1999 Dec)
    Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR