D2hgdh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	D2hgdh
Entrez Gene ID	31184
Full Name	D-2-hydroxyglutaric acid dehydrogenase
Synonyms	CG3835,D2HGDH,Dmel\CG3835,EG87B1,EG:87B1.3
General protein information	Preferred Names D-2-hydroxyglutaric acid dehydrogenase Names CG3835 gene product from transcript CG3835-RC CG3835-PA CG3835-PB CG3835-PC D2hgdh-PA D2hgdh-PB D2hgdh-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2D4-2D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_130626.3	NP_569982.1	D-2-hydroxyglutaric acid dehydrogenase, isoform A
NM_166930.2	NP_726795.1	D-2-hydroxyglutaric acid dehydrogenase, isoform C
NM_166929.2	NP_726794.1	D-2-hydroxyglutaric acid dehydrogenase, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	D2HGDH	XP_001162772.3
Rattus norvegicus (Norway rat)	D2hgdh	NP_001100396.1
Danio rerio (zebrafish)	d2hgdh	NP_001074066.1
Kluyveromyces lactis	KLLA0F05753g	XP_455341.1
Schizosaccharomyces pombe (fission yeast)	SPBC713.03	NP_595342.1
	LOC702028	XP_001094865.2
Bos taurus (cattle)	D2HGDH	NP_001069446.1
Mus musculus (house mouse)	D2hgdh	NP_849213.2
Gallus gallus (chicken)	D2HGDH	XP_422664.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004189	XP_001230873.3
Eremothecium gossypii	AGOS_ACL021C	NP_983383.1
Canis lupus familiaris (dog)	D2HGDH	XP_005636042.1
Caenorhabditis elegans (roundworm)	CELE_F54D5.12	NP_496465.1
Magnaporthe oryzae (rice blast fungus)	MGG_12830	XP_003717091.1
Arabidopsis thaliana (thale cress)	AT4G36400	NP_568003.2
Homo sapiens (human)	D2HGDH	NP_689996.4
Drosophila melanogaster (fruit fly)	CG3835	NP_569982.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004195	XP_313089.4
Saccharomyces cerevisiae (baker's yeast)	DLD2	NP_010103.1
Neurospora crassa	NCU06441	XP_957332.1
Xenopus tropicalis (tropical clawed frog)	d2hgdh	XP_002936654.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We identified the fly homolog of D-2-hydroxyglutaric acid dehydrogenase (CG3835), which metabolizes D-2HG, and showed that coexpression of this enzyme with mutant Idh abolishes mutant Idh-associated phenotype
Title: Genetic dissection of leukemia-associated IDH1 and IDH2 mutants and D-2-hydroxyglutarate in Drosophila.

The following D2hgdh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the D2hgdh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00560	NM_130626.3 Latest version!	Drosophila melanogaster D-2-hydroxyglutaric acid dehydrogenase (D2hgdh), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa00560	NM_166929.2 Latest version!	Drosophila melanogaster D-2-hydroxyglutaric acid dehydrogenase (D2hgdh), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa00560	NM_166930.2 Latest version!	Drosophila melanogaster D-2-hydroxyglutaric acid dehydrogenase (D2hgdh), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

D2hgdh ( NM_130626.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569982.1 Drosophila melanogaster D-2-hydroxyglutaric acid dehydrogenase (D2hgdh), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00560
Clone ID Related Accession (Same CDS sequence)	NM_130626.3 , NM_166930.2 , NM_166929.2
Accession Version	NM_130626.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	D-2-hydroxyglutaric acid dehydrogenase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_130626.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTCC AACAGCTCCT GCATGTGCGC AGATTGGCCA GCGCCGGGGC CACCACCTTG 
ATGAGGCGGC CTATCACCAC CACCACCATC ACCACCACCA GTCGCACCGC CCTGGCAGCC 
ACCTACCCCG CTTTCGCAAC CACTGTGCGC ACAATGGCGG GCAGCGGGGC TCCGATCCCG 
GAGCTCACAG AGATCACCGA CAACGTGCAG CGCGGCAACT ACGCCACTCT GACCGACAAG 
GATGTGGCGC ATTTCGAGCA GCTCCTGGGC AAGAACTTCG TGCTCACTGA GGACCTGGAG 
GGATACAACA TCTGCTTCCT TAAGAGGATT CGAGGCAACA GCAAGTTGGT GCTTAAGCCC 
GGAAGCACGG CGGAGGTGGC CGCCATCCTG AAGTACTGCA ACGAGCGTCG TTTGGCGGTG 
GTGCCGCAGG GCGGGAACAC AGGTCTAGTG GGCGGATCCG TGCCGATCTG CGACGAGATT 
GTCCTTTCTC TAGCGCGCCT GAACAAGGTG TTATCCGTGG ACGAGGTCAC CGGCATTGCT 
GTCGTGGAGG CGGGCTGCAT CCTGGAGAAC TTCGATCAGA GGGCCAGAGA GGTGGGCTTG 
ACGGTGCCAC TGGACCTGGG CGCCAAGGCC AGTTGCCACA TCGGGGGCAA TGTGTCCACA 
AACGCGGGCG GAGTGCGGGT GGTGCGTTAC GGCAATCTGC ACGGCTCTGT TTTGGGCGTG 
GAGGCGGTGC TGGCCACCGG TCAGGTGCTG GACCTTATGT CCAACTTCAA GAAGGACAAC 
ACCGGCTACC ACATGAAGCA CTTGTTCATA GGATCCGAGG GCACTCTGGG CGTGGTCACG 
AAGCTTTCGA TGCTCTGCCC CCATTCCTCG CGAGCGGTGA ACGTGGCCTT CATCGGCCTG 
AACTCCTTCG ACGATGTGCT GAAGACTTTT GTCAGTGCCA AGCGTAATCT GGGCGAGATT 
CTAAGCTCCT GCGAGCTGAT TGACGAGCGG GCCTTGAACA CCGCCCTCGA GCAGTTCAAG 
TTCCTGAACT CCCCCATTTC GGGATTTCCC TTCTACATGC TCATCGAGAC CTCGGGCAGC 
AACGGTGACC ACGACGAGGA GAAGATCAAC CAGTTCATTG GGGACGGTAT GGAGCGTGGC 
GAGATCCAGG ATGGCACCGT AACCGGTGAT CCCGGCAAGG TGCAGGAGAT CTGGAAGATC 
CGCGAAATGG TGCCGCTGGG TCTGATCGAG AAGAGCTTCT GCTTCAAGTA CGACATCTCG 
CTGCCTCTGC GGGACTTCTA CAACATTGTG GACGTGATGC GAGAGAGGTG CGGTCCCCTG 
GCCACAGTTG TCTGCGGATA CGGCCATCTG GGGGACTCTA ATCTGCACCT GAACGTCTCC 
TGCGAGGAGT TTAACGGCGA GATCTACAAG CGGGTCGAAC CCTTCGTCTA CGAGTACACC 
TCCAAGCTGA AGGGCAGCAT TAGTGCGGAG CACGGCATTG GCTTCCTGAA GAAGGACTAC 
CTGCACTACT CCAAGGACCC GGTGGCCATT GGCTACATGC GCGAGATGAA GAAGCTGCTG 
GACCCCAACA GCATCCTCAA TCCCTATAAG GTGCTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569982.1
CDS	309..1910
Translation	MRFQQLLHVRRLASAGATTLMRRPITTTTITTTSRTALAATYPAFATTVRTMAGSGAPIPELTEITDNVQRGNYATLTDKDVAHFEQLLGKNFVLTEDLEGYNICFLKRIRGNSKLVLKPGSTAEVAAILKYCNERRLAVVPQGGNTGLVGGSVPICDEIVLSLARLNKVLSVDEVTGIAVVEAGCILENFDQRAREVGLTVPLDLGAKASCHIGGNVSTNAGGVRVVRYGNLHGSVLGVEAVLATGQVLDLMSNFKKDNTGYHMKHLFIGSEGTLGVVTKLSMLCPHSSRAVNVAFIGLNSFDDVLKTFVSAKRNLGEILSSCELIDERALNTALEQFKFLNSPISGFPFYMLIETSGSNGDHDEEKINQFIGDGMERGEIQDGTVTGDPGKVQEIWKIREMVPLGLIEKSFCFKYDISLPLRDFYNIVDVMRERCGPLATVVCGYGHLGDSNLHLNVSCEEFNGEIYKRVEPFVYEYTSKLKGSISAEHGIGFLKKDYLHYSKDPVAIGYMREMKKLLDPNSILNPYKVLN

Target ORF information:

RefSeq Version	NM_130626.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster D-2-hydroxyglutaric acid dehydrogenase (D2hgdh), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130626.3

ORF Insert Sequence:

ATGAGATTCC AACAGCTCCT GCATGTGCGC AGATTGGCCA GCGCCGGGGC CACCACCTTG 
ATGAGGCGGC CTATCACCAC CACCACCATC ACCACCACCA GTCGCACCGC CCTGGCAGCC 
ACCTACCCCG CTTTCGCAAC CACTGTGCGC ACAATGGCGG GCAGCGGGGC TCCGATCCCG 
GAGCTCACAG AGATCACCGA CAACGTGCAG CGCGGCAACT ACGCCACTCT GACCGACAAG 
GATGTGGCGC ATTTCGAGCA GCTCCTGGGC AAGAACTTCG TGCTCACTGA GGACCTGGAG 
GGATACAACA TCTGCTTCCT TAAGAGGATT CGAGGCAACA GCAAGTTGGT GCTTAAGCCC 
GGAAGCACGG CGGAGGTGGC CGCCATCCTG AAGTACTGCA ACGAGCGTCG TTTGGCGGTG 
GTGCCGCAGG GCGGGAACAC AGGTCTAGTG GGCGGATCCG TGCCGATCTG CGACGAGATT 
GTCCTTTCTC TAGCGCGCCT GAACAAGGTG TTATCCGTGG ACGAGGTCAC CGGCATTGCT 
GTCGTGGAGG CGGGCTGCAT CCTGGAGAAC TTCGATCAGA GGGCCAGAGA GGTGGGCTTG 
ACGGTGCCAC TGGACCTGGG CGCCAAGGCC AGTTGCCACA TCGGGGGCAA TGTGTCCACA 
AACGCGGGCG GAGTGCGGGT GGTGCGTTAC GGCAATCTGC ACGGCTCTGT TTTGGGCGTG 
GAGGCGGTGC TGGCCACCGG TCAGGTGCTG GACCTTATGT CCAACTTCAA GAAGGACAAC 
ACCGGCTACC ACATGAAGCA CTTGTTCATA GGATCCGAGG GCACTCTGGG CGTGGTCACG 
AAGCTTTCGA TGCTCTGCCC CCATTCCTCG CGAGCGGTGA ACGTGGCCTT CATCGGCCTG 
AACTCCTTCG ACGATGTGCT GAAGACTTTT GTCAGTGCCA AGCGTAATCT GGGCGAGATT 
CTAAGCTCCT GCGAGCTGAT TGACGAGCGG GCCTTGAACA CCGCCCTCGA GCAGTTCAAG 
TTCCTGAACT CCCCCATTTC GGGATTTCCC TTCTACATGC TCATCGAGAC CTCGGGCAGC 
AACGGTGACC ACGACGAGGA GAAGATCAAC CAGTTCATTG GGGACGGTAT GGAGCGTGGC 
GAGATCCAGG ATGGCACCGT AACCGGTGAT CCCGGCAAGG TGCAGGAGAT CTGGAAGATC 
CGCGAAATGG TGCCGCTGGG TCTGATCGAG AAGAGCTTCT GCTTCAAGTA CGACATCTCG 
CTGCCTCTGC GGGACTTCTA CAACATTGTG GACGTGATGC GAGAGAGGTG CGGTCCCCTG 
GCCACAGTTG TCTGCGGATA CGGCCATCTG GGGGACTCTA ATCTGCACCT GAACGTCTCC 
TGCGAGGAGT TTAACGGCGA GATCTACAAG CGGGTCGAAC CCTTCGTCTA CGAGTACACC 
TCCAAGCTGA AGGGCAGCAT TAGTGCGGAG CACGGCATTG GCTTCCTGAA GAAGGACTAC 
CTGCACTACT CCAAGGACCC GGTGGCCATT GGCTACATGC GCGAGATGAA GAAGCTGCTG 
GACCCCAACA GCATCCTCAA TCCCTATAAG GTGCTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

D2hgdh ( NM_166930.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726795.1 Drosophila melanogaster D-2-hydroxyglutaric acid dehydrogenase (D2hgdh), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00560
Clone ID Related Accession (Same CDS sequence)	NM_130626.3 , NM_166930.2 , NM_166929.2
Accession Version	NM_166930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	D-2-hydroxyglutaric acid dehydrogenase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_166930.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTCC AACAGCTCCT GCATGTGCGC AGATTGGCCA GCGCCGGGGC CACCACCTTG 
ATGAGGCGGC CTATCACCAC CACCACCATC ACCACCACCA GTCGCACCGC CCTGGCAGCC 
ACCTACCCCG CTTTCGCAAC CACTGTGCGC ACAATGGCGG GCAGCGGGGC TCCGATCCCG 
GAGCTCACAG AGATCACCGA CAACGTGCAG CGCGGCAACT ACGCCACTCT GACCGACAAG 
GATGTGGCGC ATTTCGAGCA GCTCCTGGGC AAGAACTTCG TGCTCACTGA GGACCTGGAG 
GGATACAACA TCTGCTTCCT TAAGAGGATT CGAGGCAACA GCAAGTTGGT GCTTAAGCCC 
GGAAGCACGG CGGAGGTGGC CGCCATCCTG AAGTACTGCA ACGAGCGTCG TTTGGCGGTG 
GTGCCGCAGG GCGGGAACAC AGGTCTAGTG GGCGGATCCG TGCCGATCTG CGACGAGATT 
GTCCTTTCTC TAGCGCGCCT GAACAAGGTG TTATCCGTGG ACGAGGTCAC CGGCATTGCT 
GTCGTGGAGG CGGGCTGCAT CCTGGAGAAC TTCGATCAGA GGGCCAGAGA GGTGGGCTTG 
ACGGTGCCAC TGGACCTGGG CGCCAAGGCC AGTTGCCACA TCGGGGGCAA TGTGTCCACA 
AACGCGGGCG GAGTGCGGGT GGTGCGTTAC GGCAATCTGC ACGGCTCTGT TTTGGGCGTG 
GAGGCGGTGC TGGCCACCGG TCAGGTGCTG GACCTTATGT CCAACTTCAA GAAGGACAAC 
ACCGGCTACC ACATGAAGCA CTTGTTCATA GGATCCGAGG GCACTCTGGG CGTGGTCACG 
AAGCTTTCGA TGCTCTGCCC CCATTCCTCG CGAGCGGTGA ACGTGGCCTT CATCGGCCTG 
AACTCCTTCG ACGATGTGCT GAAGACTTTT GTCAGTGCCA AGCGTAATCT GGGCGAGATT 
CTAAGCTCCT GCGAGCTGAT TGACGAGCGG GCCTTGAACA CCGCCCTCGA GCAGTTCAAG 
TTCCTGAACT CCCCCATTTC GGGATTTCCC TTCTACATGC TCATCGAGAC CTCGGGCAGC 
AACGGTGACC ACGACGAGGA GAAGATCAAC CAGTTCATTG GGGACGGTAT GGAGCGTGGC 
GAGATCCAGG ATGGCACCGT AACCGGTGAT CCCGGCAAGG TGCAGGAGAT CTGGAAGATC 
CGCGAAATGG TGCCGCTGGG TCTGATCGAG AAGAGCTTCT GCTTCAAGTA CGACATCTCG 
CTGCCTCTGC GGGACTTCTA CAACATTGTG GACGTGATGC GAGAGAGGTG CGGTCCCCTG 
GCCACAGTTG TCTGCGGATA CGGCCATCTG GGGGACTCTA ATCTGCACCT GAACGTCTCC 
TGCGAGGAGT TTAACGGCGA GATCTACAAG CGGGTCGAAC CCTTCGTCTA CGAGTACACC 
TCCAAGCTGA AGGGCAGCAT TAGTGCGGAG CACGGCATTG GCTTCCTGAA GAAGGACTAC 
CTGCACTACT CCAAGGACCC GGTGGCCATT GGCTACATGC GCGAGATGAA GAAGCTGCTG 
GACCCCAACA GCATCCTCAA TCCCTATAAG GTGCTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726795.1
CDS	288..1889
Translation	MRFQQLLHVRRLASAGATTLMRRPITTTTITTTSRTALAATYPAFATTVRTMAGSGAPIPELTEITDNVQRGNYATLTDKDVAHFEQLLGKNFVLTEDLEGYNICFLKRIRGNSKLVLKPGSTAEVAAILKYCNERRLAVVPQGGNTGLVGGSVPICDEIVLSLARLNKVLSVDEVTGIAVVEAGCILENFDQRAREVGLTVPLDLGAKASCHIGGNVSTNAGGVRVVRYGNLHGSVLGVEAVLATGQVLDLMSNFKKDNTGYHMKHLFIGSEGTLGVVTKLSMLCPHSSRAVNVAFIGLNSFDDVLKTFVSAKRNLGEILSSCELIDERALNTALEQFKFLNSPISGFPFYMLIETSGSNGDHDEEKINQFIGDGMERGEIQDGTVTGDPGKVQEIWKIREMVPLGLIEKSFCFKYDISLPLRDFYNIVDVMRERCGPLATVVCGYGHLGDSNLHLNVSCEEFNGEIYKRVEPFVYEYTSKLKGSISAEHGIGFLKKDYLHYSKDPVAIGYMREMKKLLDPNSILNPYKVLN

Target ORF information:

RefSeq Version	NM_166930.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster D-2-hydroxyglutaric acid dehydrogenase (D2hgdh), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166930.2

ORF Insert Sequence:

ATGAGATTCC AACAGCTCCT GCATGTGCGC AGATTGGCCA GCGCCGGGGC CACCACCTTG 
ATGAGGCGGC CTATCACCAC CACCACCATC ACCACCACCA GTCGCACCGC CCTGGCAGCC 
ACCTACCCCG CTTTCGCAAC CACTGTGCGC ACAATGGCGG GCAGCGGGGC TCCGATCCCG 
GAGCTCACAG AGATCACCGA CAACGTGCAG CGCGGCAACT ACGCCACTCT GACCGACAAG 
GATGTGGCGC ATTTCGAGCA GCTCCTGGGC AAGAACTTCG TGCTCACTGA GGACCTGGAG 
GGATACAACA TCTGCTTCCT TAAGAGGATT CGAGGCAACA GCAAGTTGGT GCTTAAGCCC 
GGAAGCACGG CGGAGGTGGC CGCCATCCTG AAGTACTGCA ACGAGCGTCG TTTGGCGGTG 
GTGCCGCAGG GCGGGAACAC AGGTCTAGTG GGCGGATCCG TGCCGATCTG CGACGAGATT 
GTCCTTTCTC TAGCGCGCCT GAACAAGGTG TTATCCGTGG ACGAGGTCAC CGGCATTGCT 
GTCGTGGAGG CGGGCTGCAT CCTGGAGAAC TTCGATCAGA GGGCCAGAGA GGTGGGCTTG 
ACGGTGCCAC TGGACCTGGG CGCCAAGGCC AGTTGCCACA TCGGGGGCAA TGTGTCCACA 
AACGCGGGCG GAGTGCGGGT GGTGCGTTAC GGCAATCTGC ACGGCTCTGT TTTGGGCGTG 
GAGGCGGTGC TGGCCACCGG TCAGGTGCTG GACCTTATGT CCAACTTCAA GAAGGACAAC 
ACCGGCTACC ACATGAAGCA CTTGTTCATA GGATCCGAGG GCACTCTGGG CGTGGTCACG 
AAGCTTTCGA TGCTCTGCCC CCATTCCTCG CGAGCGGTGA ACGTGGCCTT CATCGGCCTG 
AACTCCTTCG ACGATGTGCT GAAGACTTTT GTCAGTGCCA AGCGTAATCT GGGCGAGATT 
CTAAGCTCCT GCGAGCTGAT TGACGAGCGG GCCTTGAACA CCGCCCTCGA GCAGTTCAAG 
TTCCTGAACT CCCCCATTTC GGGATTTCCC TTCTACATGC TCATCGAGAC CTCGGGCAGC 
AACGGTGACC ACGACGAGGA GAAGATCAAC CAGTTCATTG GGGACGGTAT GGAGCGTGGC 
GAGATCCAGG ATGGCACCGT AACCGGTGAT CCCGGCAAGG TGCAGGAGAT CTGGAAGATC 
CGCGAAATGG TGCCGCTGGG TCTGATCGAG AAGAGCTTCT GCTTCAAGTA CGACATCTCG 
CTGCCTCTGC GGGACTTCTA CAACATTGTG GACGTGATGC GAGAGAGGTG CGGTCCCCTG 
GCCACAGTTG TCTGCGGATA CGGCCATCTG GGGGACTCTA ATCTGCACCT GAACGTCTCC 
TGCGAGGAGT TTAACGGCGA GATCTACAAG CGGGTCGAAC CCTTCGTCTA CGAGTACACC 
TCCAAGCTGA AGGGCAGCAT TAGTGCGGAG CACGGCATTG GCTTCCTGAA GAAGGACTAC 
CTGCACTACT CCAAGGACCC GGTGGCCATT GGCTACATGC GCGAGATGAA GAAGCTGCTG 
GACCCCAACA GCATCCTCAA TCCCTATAAG GTGCTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

D2hgdh ( NM_166929.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726794.1 Drosophila melanogaster D-2-hydroxyglutaric acid dehydrogenase (D2hgdh), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00560
Clone ID Related Accession (Same CDS sequence)	NM_130626.3 , NM_166930.2 , NM_166929.2
Accession Version	NM_166929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	D-2-hydroxyglutaric acid dehydrogenase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_166929.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGATTCC AACAGCTCCT GCATGTGCGC AGATTGGCCA GCGCCGGGGC CACCACCTTG 
ATGAGGCGGC CTATCACCAC CACCACCATC ACCACCACCA GTCGCACCGC CCTGGCAGCC 
ACCTACCCCG CTTTCGCAAC CACTGTGCGC ACAATGGCGG GCAGCGGGGC TCCGATCCCG 
GAGCTCACAG AGATCACCGA CAACGTGCAG CGCGGCAACT ACGCCACTCT GACCGACAAG 
GATGTGGCGC ATTTCGAGCA GCTCCTGGGC AAGAACTTCG TGCTCACTGA GGACCTGGAG 
GGATACAACA TCTGCTTCCT TAAGAGGATT CGAGGCAACA GCAAGTTGGT GCTTAAGCCC 
GGAAGCACGG CGGAGGTGGC CGCCATCCTG AAGTACTGCA ACGAGCGTCG TTTGGCGGTG 
GTGCCGCAGG GCGGGAACAC AGGTCTAGTG GGCGGATCCG TGCCGATCTG CGACGAGATT 
GTCCTTTCTC TAGCGCGCCT GAACAAGGTG TTATCCGTGG ACGAGGTCAC CGGCATTGCT 
GTCGTGGAGG CGGGCTGCAT CCTGGAGAAC TTCGATCAGA GGGCCAGAGA GGTGGGCTTG 
ACGGTGCCAC TGGACCTGGG CGCCAAGGCC AGTTGCCACA TCGGGGGCAA TGTGTCCACA 
AACGCGGGCG GAGTGCGGGT GGTGCGTTAC GGCAATCTGC ACGGCTCTGT TTTGGGCGTG 
GAGGCGGTGC TGGCCACCGG TCAGGTGCTG GACCTTATGT CCAACTTCAA GAAGGACAAC 
ACCGGCTACC ACATGAAGCA CTTGTTCATA GGATCCGAGG GCACTCTGGG CGTGGTCACG 
AAGCTTTCGA TGCTCTGCCC CCATTCCTCG CGAGCGGTGA ACGTGGCCTT CATCGGCCTG 
AACTCCTTCG ACGATGTGCT GAAGACTTTT GTCAGTGCCA AGCGTAATCT GGGCGAGATT 
CTAAGCTCCT GCGAGCTGAT TGACGAGCGG GCCTTGAACA CCGCCCTCGA GCAGTTCAAG 
TTCCTGAACT CCCCCATTTC GGGATTTCCC TTCTACATGC TCATCGAGAC CTCGGGCAGC 
AACGGTGACC ACGACGAGGA GAAGATCAAC CAGTTCATTG GGGACGGTAT GGAGCGTGGC 
GAGATCCAGG ATGGCACCGT AACCGGTGAT CCCGGCAAGG TGCAGGAGAT CTGGAAGATC 
CGCGAAATGG TGCCGCTGGG TCTGATCGAG AAGAGCTTCT GCTTCAAGTA CGACATCTCG 
CTGCCTCTGC GGGACTTCTA CAACATTGTG GACGTGATGC GAGAGAGGTG CGGTCCCCTG 
GCCACAGTTG TCTGCGGATA CGGCCATCTG GGGGACTCTA ATCTGCACCT GAACGTCTCC 
TGCGAGGAGT TTAACGGCGA GATCTACAAG CGGGTCGAAC CCTTCGTCTA CGAGTACACC 
TCCAAGCTGA AGGGCAGCAT TAGTGCGGAG CACGGCATTG GCTTCCTGAA GAAGGACTAC 
CTGCACTACT CCAAGGACCC GGTGGCCATT GGCTACATGC GCGAGATGAA GAAGCTGCTG 
GACCCCAACA GCATCCTCAA TCCCTATAAG GTGCTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726794.1
CDS	184..1785
Translation	MRFQQLLHVRRLASAGATTLMRRPITTTTITTTSRTALAATYPAFATTVRTMAGSGAPIPELTEITDNVQRGNYATLTDKDVAHFEQLLGKNFVLTEDLEGYNICFLKRIRGNSKLVLKPGSTAEVAAILKYCNERRLAVVPQGGNTGLVGGSVPICDEIVLSLARLNKVLSVDEVTGIAVVEAGCILENFDQRAREVGLTVPLDLGAKASCHIGGNVSTNAGGVRVVRYGNLHGSVLGVEAVLATGQVLDLMSNFKKDNTGYHMKHLFIGSEGTLGVVTKLSMLCPHSSRAVNVAFIGLNSFDDVLKTFVSAKRNLGEILSSCELIDERALNTALEQFKFLNSPISGFPFYMLIETSGSNGDHDEEKINQFIGDGMERGEIQDGTVTGDPGKVQEIWKIREMVPLGLIEKSFCFKYDISLPLRDFYNIVDVMRERCGPLATVVCGYGHLGDSNLHLNVSCEEFNGEIYKRVEPFVYEYTSKLKGSISAEHGIGFLKKDYLHYSKDPVAIGYMREMKKLLDPNSILNPYKVLN

Target ORF information:

RefSeq Version	NM_166929.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster D-2-hydroxyglutaric acid dehydrogenase (D2hgdh), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166929.2

ORF Insert Sequence:

ATGAGATTCC AACAGCTCCT GCATGTGCGC AGATTGGCCA GCGCCGGGGC CACCACCTTG 
ATGAGGCGGC CTATCACCAC CACCACCATC ACCACCACCA GTCGCACCGC CCTGGCAGCC 
ACCTACCCCG CTTTCGCAAC CACTGTGCGC ACAATGGCGG GCAGCGGGGC TCCGATCCCG 
GAGCTCACAG AGATCACCGA CAACGTGCAG CGCGGCAACT ACGCCACTCT GACCGACAAG 
GATGTGGCGC ATTTCGAGCA GCTCCTGGGC AAGAACTTCG TGCTCACTGA GGACCTGGAG 
GGATACAACA TCTGCTTCCT TAAGAGGATT CGAGGCAACA GCAAGTTGGT GCTTAAGCCC 
GGAAGCACGG CGGAGGTGGC CGCCATCCTG AAGTACTGCA ACGAGCGTCG TTTGGCGGTG 
GTGCCGCAGG GCGGGAACAC AGGTCTAGTG GGCGGATCCG TGCCGATCTG CGACGAGATT 
GTCCTTTCTC TAGCGCGCCT GAACAAGGTG TTATCCGTGG ACGAGGTCAC CGGCATTGCT 
GTCGTGGAGG CGGGCTGCAT CCTGGAGAAC TTCGATCAGA GGGCCAGAGA GGTGGGCTTG 
ACGGTGCCAC TGGACCTGGG CGCCAAGGCC AGTTGCCACA TCGGGGGCAA TGTGTCCACA 
AACGCGGGCG GAGTGCGGGT GGTGCGTTAC GGCAATCTGC ACGGCTCTGT TTTGGGCGTG 
GAGGCGGTGC TGGCCACCGG TCAGGTGCTG GACCTTATGT CCAACTTCAA GAAGGACAAC 
ACCGGCTACC ACATGAAGCA CTTGTTCATA GGATCCGAGG GCACTCTGGG CGTGGTCACG 
AAGCTTTCGA TGCTCTGCCC CCATTCCTCG CGAGCGGTGA ACGTGGCCTT CATCGGCCTG 
AACTCCTTCG ACGATGTGCT GAAGACTTTT GTCAGTGCCA AGCGTAATCT GGGCGAGATT 
CTAAGCTCCT GCGAGCTGAT TGACGAGCGG GCCTTGAACA CCGCCCTCGA GCAGTTCAAG 
TTCCTGAACT CCCCCATTTC GGGATTTCCC TTCTACATGC TCATCGAGAC CTCGGGCAGC 
AACGGTGACC ACGACGAGGA GAAGATCAAC CAGTTCATTG GGGACGGTAT GGAGCGTGGC 
GAGATCCAGG ATGGCACCGT AACCGGTGAT CCCGGCAAGG TGCAGGAGAT CTGGAAGATC 
CGCGAAATGG TGCCGCTGGG TCTGATCGAG AAGAGCTTCT GCTTCAAGTA CGACATCTCG 
CTGCCTCTGC GGGACTTCTA CAACATTGTG GACGTGATGC GAGAGAGGTG CGGTCCCCTG 
GCCACAGTTG TCTGCGGATA CGGCCATCTG GGGGACTCTA ATCTGCACCT GAACGTCTCC 
TGCGAGGAGT TTAACGGCGA GATCTACAAG CGGGTCGAAC CCTTCGTCTA CGAGTACACC 
TCCAAGCTGA AGGGCAGCAT TAGTGCGGAG CACGGCATTG GCTTCCTGAA GAAGGACTAC 
CTGCACTACT CCAAGGACCC GGTGGCCATT GGCTACATGC GCGAGATGAA GAAGCTGCTG 
GACCCCAACA GCATCCTCAA TCCCTATAAG GTGCTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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D2hgdh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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