FLDH cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following FLDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FLDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb17059 NM_119490.2
Latest version!
Arabidopsis thaliana NAD(P)-binding Rossmann-fold superfamily protein (FLDH), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OAb17060 NM_001125637.1
Latest version!
Arabidopsis thaliana NAD(P)-binding Rossmann-fold superfamily protein (FLDH), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OAb30197 NM_001203973.1
Latest version!
Arabidopsis thaliana NAD(P)-binding Rossmann-fold superfamily protein (FLDH), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb17059
    Clone ID Related Accession (Same CDS sequence) NM_119490.2
    Accession Version NM_119490.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product NAD(P)-binding Rossmann-fold superfamily protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On May 13, 2003 this sequence version replaced NM_119490.1. COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGGCCCAA AGATGCCCAA CACAGAAACA GAAAATATGA AAATATTAGT CACCGGCTCT 
    ACTGGTTACT TAGGCGCACG ACTCTGCCAC GTTCTCCTCC GTCGTGGCCA CTCCGTACGT
    GCATTGGTCC GCCGCACCAG CGATCTCTCT GACTTACCTC CAGAAGTTGA GCTCGCCTAT
    GGAGACGTAA CGGATTACCG TTCTCTAACG GATGCTTGCT CCGGTTGCGA TATCGTTTTC
    CACGCCGCAG CTTTAGTTGA GCCTTGGCTT CCTGACCCAT CTCGATTCAT CTCAGTCAAC
    GTTGGTGGAC TGAAGAATGT CTTGGAAGCT GTAAAGGAGA CGAAGACCGT GCAGAAGATT
    ATCTACACTT CTTCCTTCTT CGCTCTTGGA TCTACCGATG GATCTGTTGC TAATGAGAAT
    CAAGTTCATA ATGAGAGGTT CTTCTGCACT GAGTATGAGA GATCCAAGGC TGTTGCTGAT
    AAGATGGCTT TGAATGCTGC TTCTGAAGGT GTGCCGATAA TTTTGCTGTA TCCTGGTGTT
    ATTTTTGGTC CAGGCAAGCT CACTTCAGCC AATATGGTTG CTAGAATGTT GATCGAGCGC
    TTTAACGGGA GGTTGCCTGG ATATATTGGC TCTGGGACGG ATAGATACTC CTTCAGCCAT
    GTTGATGATG TTGTGGAGGG TCATGTTGCT GCAATGGAAA AAGGTAGGCT TGGTGAAAGA
    TACTTACTCA CTGGGGAAAA TGCTTCTTTC AAGCTTGTCT TTGACATGGC TGCACTGATT
    ACTGGAACCA AAAAGCCAAA CTTTAGCATT CCATTATGGG CCATCAACGC ATACGGATGG
    TTATCTGTTT TAATCTCTCG GGTTACAGGA AAGCTTCCAC TTATCAGTCC TCCGACAGTG
    ACAGTGCTGA GACATCAGTG GTCATACTCT TGTGATAAGG CTAAGCTGGA ACTTGGTTAT
    AACCCTCGAA GCCTAAAGGA AGGATTGGAG GAGATGCTCC CTTGGTTGAA GAGTCTGGGC
    GTCATTCACT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_195062.1
    CDS1..1035
    Translation

    Target ORF information:

    RefSeq Version NM_119490.2
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana NAD(P)-binding Rossmann-fold superfamily protein (FLDH), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_119490.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGGCCCAA AGATGCCCAA CACAGAAACA GAAAATATGA AAATATTAGT CACCGGCTCT 
    ACTGGTTACT TAGGCGCACG ACTCTGCCAC GTTCTCCTCC GTCGTGGCCA CTCCGTACGT
    GCATTGGTCC GCCGCACCAG CGATCTCTCT GACTTACCTC CAGAAGTTGA GCTCGCCTAT
    GGAGACGTAA CGGATTACCG TTCTCTAACG GATGCTTGCT CCGGTTGCGA TATCGTTTTC
    CACGCCGCAG CTTTAGTTGA GCCTTGGCTT CCTGACCCAT CTCGATTCAT CTCAGTCAAC
    GTTGGTGGAC TGAAGAATGT CTTGGAAGCT GTAAAGGAGA CGAAGACCGT GCAGAAGATT
    ATCTACACTT CTTCCTTCTT CGCTCTTGGA TCTACCGATG GATCTGTTGC TAATGAGAAT
    CAAGTTCATA ATGAGAGGTT CTTCTGCACT GAGTATGAGA GATCCAAGGC TGTTGCTGAT
    AAGATGGCTT TGAATGCTGC TTCTGAAGGT GTGCCGATAA TTTTGCTGTA TCCTGGTGTT
    ATTTTTGGTC CAGGCAAGCT CACTTCAGCC AATATGGTTG CTAGAATGTT GATCGAGCGC
    TTTAACGGGA GGTTGCCTGG ATATATTGGC TCTGGGACGG ATAGATACTC CTTCAGCCAT
    GTTGATGATG TTGTGGAGGG TCATGTTGCT GCAATGGAAA AAGGTAGGCT TGGTGAAAGA
    TACTTACTCA CTGGGGAAAA TGCTTCTTTC AAGCTTGTCT TTGACATGGC TGCACTGATT
    ACTGGAACCA AAAAGCCAAA CTTTAGCATT CCATTATGGG CCATCAACGC ATACGGATGG
    TTATCTGTTT TAATCTCTCG GGTTACAGGA AAGCTTCCAC TTATCAGTCC TCCGACAGTG
    ACAGTGCTGA GACATCAGTG GTCATACTCT TGTGATAAGG CTAAGCTGGA ACTTGGTTAT
    AACCCTCGAA GCCTAAAGGA AGGATTGGAG GAGATGCTCC CTTGGTTGAA GAGTCTGGGC
    GTCATTCACT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb17060
    Clone ID Related Accession (Same CDS sequence) NM_001125637.1
    Accession Version NM_001125637.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product NAD(P)-binding Rossmann-fold superfamily protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). COMPLETENESS: incomplete on the 5' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGCCCAA AGATGCCCAA CACAGAAACA GAAAATATGA AAATATTAGT CACCGGCTCT 
    ACTGGTTACT TAGGCGCACG ACTCTGCCAC GTTCTCCTCC GTCGTGGCCA CTCCGTACGT
    GCATTGGTCC GCCGCACCAG CGATCTCTCT GACTTACCTC CAGAAGTTGA GCTCGCCTAT
    GGAGACGTAA CGGATTACCG TTCTCTAACG GATGCTTGCT CCGGTTGCGA TATCGTTTTC
    CACGCCGCAG CTTTAGTTGA GCCTTGGCTT CCTGACCCAT CTCGATTCAT CTCAGTCAAC
    GTTGGTGGAC TGAAGAATGT CTTGGAAGCT GTAAAGGAGA CGAAGACCGT GCAGAAGATT
    ATCTACACTT CTTCCTTCTT CGCTCTTGGA TCTACCGATG GATCTGTTGC TAATGAGAAT
    CAAGTTCATA ATGAGAGGTT CTTCTGCACT GAGTATGAGA GATCCAAGGC TGTTGCTGAT
    AAGATGGCTT TGAATGCTGC TTCTGAAGGT GTGCCGATAA TTTTGCTGTA TCCTGGTGTT
    ATTTTTGGTC CAGGCAAGCT CACTTCAGCC AATATGGTTG CTAGAATGTT GATCGAGCGC
    TTTAACGGGA GGTTGCCTGG ATATATTGGC TCTGGGACGG ATAGATACTC CTTCAGCCAT
    GTTGATGATG TTGTGGAGGG TCATGTTGCT GCAATGGAAA AAGGTAGGCT TGGTGAAAGA
    TACTTACTCA CTGGGGAAAA TGCTTCTTTC AAGCTTGTCT TTGACATGGC TGCACTGATT
    ACTGGAACCA AAAAGCCAAA CTTTAGCATT CCATTATGGG CCATCAACGC ATACGGATGG
    TTATCTGTTT TAATCTCTCG GGTTACAGGA AAGCTTCCAC TTATCAGTCC TCCGTATTTT
    GTGATGAACA GACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001119109.1
    CDS1..918
    Translation

    Target ORF information:

    RefSeq Version NM_001125637.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana NAD(P)-binding Rossmann-fold superfamily protein (FLDH), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001125637.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGCCCAA AGATGCCCAA CACAGAAACA GAAAATATGA AAATATTAGT CACCGGCTCT 
    ACTGGTTACT TAGGCGCACG ACTCTGCCAC GTTCTCCTCC GTCGTGGCCA CTCCGTACGT
    GCATTGGTCC GCCGCACCAG CGATCTCTCT GACTTACCTC CAGAAGTTGA GCTCGCCTAT
    GGAGACGTAA CGGATTACCG TTCTCTAACG GATGCTTGCT CCGGTTGCGA TATCGTTTTC
    CACGCCGCAG CTTTAGTTGA GCCTTGGCTT CCTGACCCAT CTCGATTCAT CTCAGTCAAC
    GTTGGTGGAC TGAAGAATGT CTTGGAAGCT GTAAAGGAGA CGAAGACCGT GCAGAAGATT
    ATCTACACTT CTTCCTTCTT CGCTCTTGGA TCTACCGATG GATCTGTTGC TAATGAGAAT
    CAAGTTCATA ATGAGAGGTT CTTCTGCACT GAGTATGAGA GATCCAAGGC TGTTGCTGAT
    AAGATGGCTT TGAATGCTGC TTCTGAAGGT GTGCCGATAA TTTTGCTGTA TCCTGGTGTT
    ATTTTTGGTC CAGGCAAGCT CACTTCAGCC AATATGGTTG CTAGAATGTT GATCGAGCGC
    TTTAACGGGA GGTTGCCTGG ATATATTGGC TCTGGGACGG ATAGATACTC CTTCAGCCAT
    GTTGATGATG TTGTGGAGGG TCATGTTGCT GCAATGGAAA AAGGTAGGCT TGGTGAAAGA
    TACTTACTCA CTGGGGAAAA TGCTTCTTTC AAGCTTGTCT TTGACATGGC TGCACTGATT
    ACTGGAACCA AAAAGCCAAA CTTTAGCATT CCATTATGGG CCATCAACGC ATACGGATGG
    TTATCTGTTT TAATCTCTCG GGTTACAGGA AAGCTTCCAC TTATCAGTCC TCCGTATTTT
    GTGATGAACA GACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb30197
    Clone ID Related Accession (Same CDS sequence) NM_001203973.1
    Accession Version NM_001203973.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product NAD(P)-binding Rossmann-fold superfamily protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAATAT TAGTCACCGG CTCTACTGGT TACTTAGGCG CACGACTCTG CCACGTTCTC 
    CTCCGTCGTG GCCACTCCGT ACGTGCATTG GTCCGCCGCA CCAGCGATCT CTCTGACTTA
    CCTCCAGAAG TTGAGCTCGC CTATGGAGAC GTAACGGATT ACCGTTCTCT AACGGATGCT
    TGCTCCGGTT GCGATATCGT TTTCCACGCC GCAGCTTTAG TTGAGCCTTG GCTTCCTGAC
    CCATCTCGAT TCATCTCAGT CAACGTTGGT GGACTGAAGA ATGTCTTGGA AGCTGTAAAG
    GAGACGAAGA CCGTGCAGAA GATTATCTAC ACTTCTTCCT TCTTCGCTCT TGGATCTACC
    GATGGATCTG TTGCTAATGA GAATCAAGTT CATAATGAGA GGTTCTTCTG CACTGAGTAT
    GAGAGATCCA AGGCTGTTGC TGATAAGATG GCTTTGAATG CTGCTTCTGA AGGTGTGCCG
    ATAATTTTGC TGTATCCTGG TGTTATTTTT GGTCCAGGCA AGCTCACTTC AGCCAATATG
    GTTGCTAGAA TGTTGATCGA GCGCTTTAAC GGGAGGTTGC CTGGATATAT TGGCTCTGGG
    ACGGATAGAT ACTCCTTCAG CCATGTTGAT GATGTTGTGG AGGGTCATGT TGCTGCAATG
    GAAAAAGGTA GGCTTGGTGA AAGATACTTA CTCACTGGGG AAAATGCTTC TTTCAAGCTT
    GTCTTTGACA TGGCTGCACT GATTACTGGA ACCAAAAAGC CAAACTTTAG CATTCCATTA
    TGGGCCATCA ACGCATACGG ATGGTTATCT GTTTTAATCT CTCGGGTTAC AGGAAAGCTT
    CCACTTATCA GTCCTCCGAC AGTGACAGTG CTGAGACATC AGTGGTCATA CTCTTGTGAT
    AAGGCTAAGC TGGAACTTGG TTATAACCCT CGAAGCCTAA AGGAAGGATT GGAGGAGATG
    CTCCCTTGTC ACTATGATGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001190902.1
    CDS30..1013
    Translation

    Target ORF information:

    RefSeq Version NM_001203973.1
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana NAD(P)-binding Rossmann-fold superfamily protein (FLDH), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001203973.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAATAT TAGTCACCGG CTCTACTGGT TACTTAGGCG CACGACTCTG CCACGTTCTC 
    CTCCGTCGTG GCCACTCCGT ACGTGCATTG GTCCGCCGCA CCAGCGATCT CTCTGACTTA
    CCTCCAGAAG TTGAGCTCGC CTATGGAGAC GTAACGGATT ACCGTTCTCT AACGGATGCT
    TGCTCCGGTT GCGATATCGT TTTCCACGCC GCAGCTTTAG TTGAGCCTTG GCTTCCTGAC
    CCATCTCGAT TCATCTCAGT CAACGTTGGT GGACTGAAGA ATGTCTTGGA AGCTGTAAAG
    GAGACGAAGA CCGTGCAGAA GATTATCTAC ACTTCTTCCT TCTTCGCTCT TGGATCTACC
    GATGGATCTG TTGCTAATGA GAATCAAGTT CATAATGAGA GGTTCTTCTG CACTGAGTAT
    GAGAGATCCA AGGCTGTTGC TGATAAGATG GCTTTGAATG CTGCTTCTGA AGGTGTGCCG
    ATAATTTTGC TGTATCCTGG TGTTATTTTT GGTCCAGGCA AGCTCACTTC AGCCAATATG
    GTTGCTAGAA TGTTGATCGA GCGCTTTAAC GGGAGGTTGC CTGGATATAT TGGCTCTGGG
    ACGGATAGAT ACTCCTTCAG CCATGTTGAT GATGTTGTGG AGGGTCATGT TGCTGCAATG
    GAAAAAGGTA GGCTTGGTGA AAGATACTTA CTCACTGGGG AAAATGCTTC TTTCAAGCTT
    GTCTTTGACA TGGCTGCACT GATTACTGGA ACCAAAAAGC CAAACTTTAG CATTCCATTA
    TGGGCCATCA ACGCATACGG ATGGTTATCT GTTTTAATCT CTCGGGTTAC AGGAAAGCTT
    CCACTTATCA GTCCTCCGAC AGTGACAGTG CTGAGACATC AGTGGTCATA CTCTTGTGAT
    AAGGCTAAGC TGGAACTTGG TTATAACCCT CGAAGCCTAA AGGAAGGATT GGAGGAGATG
    CTCCCTTGTC ACTATGATGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
    Nature402(6763)769-77(1999 Dec)
    Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR