AT4G02030 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT4G02030
Entrez Gene ID	828155
Full Name	Vps51/Vps67 family (components of vesicular transport) protein
Synonyms	T10M13.4,T10M13_4,UNH,UNHINGED,VPS51,vacuolar protein sorting 51
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001203725.1	NP_001190654.1	Vps51/Vps67 family (components of vesicular transport) protein
NM_116434.4	NP_192112.2	Vps51/Vps67 family (components of vesicular transport) protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0737800	NP_001051205.1
Arabidopsis thaliana (thale cress)	AT4G02030	NP_192112.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

UNH is important for reducing expression of PIN1 within margin cells, possibly by targeting PIN1 to the lytic vacuole. [UNHINGED] [UNH]
Title: Arabidopsis UNHINGED encodes a VPS51 homolog and reveals a role for the GARP complex in leaf shape and vein patterning.

The following AT4G02030 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G02030 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15808	NM_116434.4 Latest version!	Arabidopsis thaliana Vps51/Vps67 family (components of vesicular transport) protein (AT4G02030), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OAb29552	NM_001203725.1 Latest version!	Arabidopsis thaliana Vps51/Vps67 family (components of vesicular transport) protein (AT4G02030), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT4G02030 ( NM_116434.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_192112.2 Arabidopsis thaliana Vps51/Vps67 family (components of vesicular transport) protein (AT4G02030), mRNA.

Price & Availability↑

CloneID	OAb15808
Clone ID Related Accession (Same CDS sequence)	NM_116434.4
Accession Version	NM_116434.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Vps51/Vps67 family (components of vesicular transport) protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_116434.3.

ATGGCGACGG AGGCAGCTCC GATGGACGAA AAGGCGAAGA GAATGAGAGA TCTATTGTCG 
AGCTTCTACG CACCGGATCC TTCAATCTCG ACGAGTGGTT CCTCCATCAA CGCCTCTTTC 
GATAACATCA ACAGCACTTC CTTTGATGCT GATCAGTACA TGGATCTCAT GATCAAAAAG 
TCGAATTTGG AGGTGCTTCT GCAAAGACAT GTTCAAATGG CTGCTGAGAT TAAAAATCTC 
GACACAGACT TGCAAATGCT AGTCTATGAA AATTACAACA AGTTTATCAG TGCAACAGAT 
ACAATCAAAA GGATGAAGAG TAATATTTTC GGGATGGAAG GCAATATGGA CCAGCTTCTT 
CAGAAGATAA TGTCAGTACA ATCAAAGAGT GATGGGGTCA ACACTTCTCT TTTCGAAAAG 
AGAGAACATA TAGAGAAACT GCACCGGACT CGTAATCTTC TTCGTAAAGT TCAGTTCATC 
TATGATTTAC CTGCAAGACT GCAAAAATGT ATCAAGTCAG AAGCCTATGG CGATGCTGTC 
AGGTTCTATA CTGGAGCAAT GCCAATTCTC AAGGTATATG GCGATACATC ATTCCAAGAC 
TGCAGGCGAG CTTCCGAAGA AGCTATAGAA ATTATCATAA AGAACTTGCA GACGAAGCTA 
TTTTCAGATT CAGAATCCAT ACAGGCGAGA GCTGAAGCTG CAGTGCTTCT TAAGCAGTTA 
GATGTCCCTG TTGATAGCTT AAAGGCTAAA CTGTTGGAAA AACTGGAACA GTCTCTTGAT 
GGTCTTCAAA TAAAGCCTGA GGAGGCAAGT ACACTTGTAG AGGACGATGA TTCATCTAAC 
GATACAGAAA GCAATGACCA ACATCCTGCT AAAATTCATG AGGATGCCGT ACGTGGATTT 
TCTGAGGCCA TACGTGCTTA TCGAGAAATA TTCCCCGACT CAGAAGAAAG ACTTTTCAAA 
CTCGCGAGAG CCTTAACAGC AATGCACTTT GAGTACATGG AGCTATATAT AAAAAAACGG 
GTTTCTGCAG CAGATTTTCT AGGAATTTTT CGAATTGTAT GGGAAGATGT GGTACTTATG 
GACGAGGTGC TACCTGAGGC TGCACTCTCT GATTTATCTG CAGAGGCTGC CCAGGTTACT 
CTTAAGCAAT TTGTTGCGAG AATGTTTTCT CATCTTCAAC AGGATATATC AGATACTCTC 
TTGAAATTCG ATATCAACCA AAAGGAAGCA GTTGAAGGGG AACTCTTGAA GGTTGTCCTG 
GAAGCTAGCC AAAAAGCAGT GCTACAAGGC ACCACAAATA TATTTCAGGA CTTCCGTCAG 
CTTCTTGATG AAAAAACTGG AATATTTATA AAGATGAAGG ATTTAATCAG TGGTTGGATT 
CAGAAAGGAT CTCAAGACTT CTTCAGGTCA CTAGAAGCTC AATTTCTAGT GCTTTCTGGA 
AAAACTAGCT CATCAAACGA TATAGAGGGA AAATCGAGTG ACAAAATTCA TGCTGGTCTC 
ATTCTTGTAT TGGCGCAGCT CTCTGTCTTC ATCGAACAAA AGGTCATCCC GAGAGTTACT 
GAGGAAATAG CTGCTTCCTT CTCTGGTGGA AATTCCCAAG CCTTTGAGAA TGGACCTGCT 
TTTATTCCTG GAGAACTTTG CCGGGTCTTC CACGCAGCCA GCGAGAAACT TCTCCAGCAT 
TACATAGACA CAAGAACACA GAAGGTATCA GTTTTACTGA GAAAAAGGTT CAAGACACCT 
AACTGGGTTA AGCACAAGGA GCCACGAGAG GTTCACATGT ATGTCGATAT GTTTCTTCAC 
GAGTTAGAAG AAGTTGGTAA AGAAGTGAAA CAAGTTTTAC CTCAAGGGAC TTTCCGTAAG 
CACAAAAGAA CAGACAGCAA CGGAAGTAAC ACTACAACCT CATCACGAAG CAATACCCTC 
CATAATGATA AGATGGCACG GTCAAATTCA CAAAGAGCTC GTAGTCAGCT TTTCGAGACA 
CATCTTGCAA AACTGTTCAA GCAAAAAGTA GAAATATTCA CCAAGGTTGA ATTTACCCAG 
GAATCCGTTG TTACAACAAC AGTAAAACTT TGTCTGAAGA GTTTGCAAGA ATATGTCCGT 
CTCCAAACGT TTAACCGGAG CGGGTTCCAG CAGATTCAGC TAGACATTCA GTTCTTAAAA 
GCTCCATTGA AGGAAGCAGT TGAGGACGAA GCTGCAATAG ACTTCTTGCT CGACGAGGTG 
ATCGTTGCGG CTTCGGAGAG ATGTCTTGAT GTGATTCCAT TGGAGCCACC GATCTTGGAC 
AAACTCATAC AAGCTAAACT CGCTAAATCA AAGGAGCACA ACAACAACAC AGTTTCTTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_192112.2
CDS	181..2523
Translation	MATEAAPMDEKAKRMRDLLSSFYAPDPSISTSGSSINASFDNINSTSFDADQYMDLMIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNMDQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAYGDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVLLKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDAVRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWEDVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELLKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFLVLSGKTSSSNDIEGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKSKEHNNNTVSS

Target ORF information:

RefSeq Version	NM_116434.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Vps51/Vps67 family (components of vesicular transport) protein (AT4G02030), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_116434.4

ORF Insert Sequence:

ATGGCGACGG AGGCAGCTCC GATGGACGAA AAGGCGAAGA GAATGAGAGA TCTATTGTCG 
AGCTTCTACG CACCGGATCC TTCAATCTCG ACGAGTGGTT CCTCCATCAA CGCCTCTTTC 
GATAACATCA ACAGCACTTC CTTTGATGCT GATCAGTACA TGGATCTCAT GATCAAAAAG 
TCGAATTTGG AGGTGCTTCT GCAAAGACAT GTTCAAATGG CTGCTGAGAT TAAAAATCTC 
GACACAGACT TGCAAATGCT AGTCTATGAA AATTACAACA AGTTTATCAG TGCAACAGAT 
ACAATCAAAA GGATGAAGAG TAATATTTTC GGGATGGAAG GCAATATGGA CCAGCTTCTT 
CAGAAGATAA TGTCAGTACA ATCAAAGAGT GATGGGGTCA ACACTTCTCT TTTCGAAAAG 
AGAGAACATA TAGAGAAACT GCACCGGACT CGTAATCTTC TTCGTAAAGT TCAGTTCATC 
TATGATTTAC CTGCAAGACT GCAAAAATGT ATCAAGTCAG AAGCCTATGG CGATGCTGTC 
AGGTTCTATA CTGGAGCAAT GCCAATTCTC AAGGTATATG GCGATACATC ATTCCAAGAC 
TGCAGGCGAG CTTCCGAAGA AGCTATAGAA ATTATCATAA AGAACTTGCA GACGAAGCTA 
TTTTCAGATT CAGAATCCAT ACAGGCGAGA GCTGAAGCTG CAGTGCTTCT TAAGCAGTTA 
GATGTCCCTG TTGATAGCTT AAAGGCTAAA CTGTTGGAAA AACTGGAACA GTCTCTTGAT 
GGTCTTCAAA TAAAGCCTGA GGAGGCAAGT ACACTTGTAG AGGACGATGA TTCATCTAAC 
GATACAGAAA GCAATGACCA ACATCCTGCT AAAATTCATG AGGATGCCGT ACGTGGATTT 
TCTGAGGCCA TACGTGCTTA TCGAGAAATA TTCCCCGACT CAGAAGAAAG ACTTTTCAAA 
CTCGCGAGAG CCTTAACAGC AATGCACTTT GAGTACATGG AGCTATATAT AAAAAAACGG 
GTTTCTGCAG CAGATTTTCT AGGAATTTTT CGAATTGTAT GGGAAGATGT GGTACTTATG 
GACGAGGTGC TACCTGAGGC TGCACTCTCT GATTTATCTG CAGAGGCTGC CCAGGTTACT 
CTTAAGCAAT TTGTTGCGAG AATGTTTTCT CATCTTCAAC AGGATATATC AGATACTCTC 
TTGAAATTCG ATATCAACCA AAAGGAAGCA GTTGAAGGGG AACTCTTGAA GGTTGTCCTG 
GAAGCTAGCC AAAAAGCAGT GCTACAAGGC ACCACAAATA TATTTCAGGA CTTCCGTCAG 
CTTCTTGATG AAAAAACTGG AATATTTATA AAGATGAAGG ATTTAATCAG TGGTTGGATT 
CAGAAAGGAT CTCAAGACTT CTTCAGGTCA CTAGAAGCTC AATTTCTAGT GCTTTCTGGA 
AAAACTAGCT CATCAAACGA TATAGAGGGA AAATCGAGTG ACAAAATTCA TGCTGGTCTC 
ATTCTTGTAT TGGCGCAGCT CTCTGTCTTC ATCGAACAAA AGGTCATCCC GAGAGTTACT 
GAGGAAATAG CTGCTTCCTT CTCTGGTGGA AATTCCCAAG CCTTTGAGAA TGGACCTGCT 
TTTATTCCTG GAGAACTTTG CCGGGTCTTC CACGCAGCCA GCGAGAAACT TCTCCAGCAT 
TACATAGACA CAAGAACACA GAAGGTATCA GTTTTACTGA GAAAAAGGTT CAAGACACCT 
AACTGGGTTA AGCACAAGGA GCCACGAGAG GTTCACATGT ATGTCGATAT GTTTCTTCAC 
GAGTTAGAAG AAGTTGGTAA AGAAGTGAAA CAAGTTTTAC CTCAAGGGAC TTTCCGTAAG 
CACAAAAGAA CAGACAGCAA CGGAAGTAAC ACTACAACCT CATCACGAAG CAATACCCTC 
CATAATGATA AGATGGCACG GTCAAATTCA CAAAGAGCTC GTAGTCAGCT TTTCGAGACA 
CATCTTGCAA AACTGTTCAA GCAAAAAGTA GAAATATTCA CCAAGGTTGA ATTTACCCAG 
GAATCCGTTG TTACAACAAC AGTAAAACTT TGTCTGAAGA GTTTGCAAGA ATATGTCCGT 
CTCCAAACGT TTAACCGGAG CGGGTTCCAG CAGATTCAGC TAGACATTCA GTTCTTAAAA 
GCTCCATTGA AGGAAGCAGT TGAGGACGAA GCTGCAATAG ACTTCTTGCT CGACGAGGTG 
ATCGTTGCGG CTTCGGAGAG ATGTCTTGAT GTGATTCCAT TGGAGCCACC GATCTTGGAC 
AAACTCATAC AAGCTAAACT CGCTAAATCA AAGGAGCACA ACAACAACAC AGTTTCTTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT4G02030 ( NM_001203725.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190654.1 Arabidopsis thaliana Vps51/Vps67 family (components of vesicular transport) protein (AT4G02030), mRNA.

Price & Availability↑

CloneID	OAb29552
Clone ID Related Accession (Same CDS sequence)	NM_001203725.1
Accession Version	NM_001203725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Vps51/Vps67 family (components of vesicular transport) protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGCGACGG AGGCAGCTCC GATGGACGAA AAGGCGAAGA GAATGAGAGA TCTATTGTCG 
AGCTTCTACG CACCGGATCC TTCAATCTCG ACGAGTGGTT CCTCCATCAA CGCCTCTTTC 
GATAACATCA ACAGCACTTC CTTTGATGCT GATCAGTACA TGGATCTCAT GATTCGGCTC 
TTTGATCTTG GTCTAAGTGA TCTTCCTGCA ATGGTTGATA GTTCGGAACT AAATTGGGTT 
TATGAGATCA AAAAGTCGAA TTTGGAGGTG CTTCTGCAAA GACATGTTCA AATGGCTGCT 
GAGATTAAAA ATCTCGACAC AGACTTGCAA ATGCTAGTCT ATGAAAATTA CAACAAGTTT 
ATCAGTGCAA CAGATACAAT CAAAAGGATG AAGAGTAATA TTTTCGGGAT GGAAGGCAAT 
ATGGACCAGC TTCTTCAGAA GATAATGTCA GTACAATCAA AGAGTGATGG GGTCAACACT 
TCTCTTTTCG AAAAGAGAGA ACATATAGAG AAACTGCACC GGACTCGTAA TCTTCTTCGT 
AAAGTTCAGT TCATCTATGA TTTACCTGCA AGACTGCAAA AATGTATCAA GTCAGAAGCC 
TATGGCGATG CTGTCAGGTT CTATACTGGA GCAATGCCAA TTCTCAAGGT ATATGGCGAT 
ACATCATTCC AAGACTGCAG GCGAGCTTCC GAAGAAGCTA TAGAAATTAT CATAAAGAAC 
TTGCAGACGA AGCTATTTTC AGATTCAGAA TCCATACAGG CGAGAGCTGA AGCTGCAGTG 
CTTCTTAAGC AGTTAGATGT CCCTGTTGAT AGCTTAAAGG CTAAACTGTT GGAAAAACTG 
GAACAGTCTC TTGATGGTCT TCAAATAAAG CCTGAGGAGG CAAGTACACT TGTAGAGGAC 
GATGATTCAT CTAACGATAC AGAAAGCAAT GACCAACATC CTGCTAAAAT TCATGAGGAT 
GCCGTACGTG GATTTTCTGA GGCCATACGT GCTTATCGAG AAATATTCCC CGACTCAGAA 
GAAAGACTTT TCAAACTCGC GAGAGCCTTA ACAGCAATGC ACTTTGAGTA CATGGAGCTA 
TATATAAAAA AACGGGTTTC TGCAGCAGAT TTTCTAGGAA TTTTTCGAAT TGTATGGGAA 
GATGTGGTAC TTATGGACGA GGTGCTACCT GAGGCTGCAC TCTCTGATTT ATCTGCAGAG 
GCTGCCCAGG TTACTCTTAA GCAATTTGTT GCGAGAATGT TTTCTCATCT TCAACAGGAT 
ATATCAGATA CTCTCTTGAA ATTCGATATC AACCAAAAGG AAGCAGTTGA AGGGGAACTC 
TTGAAGGTTG TCCTGGAAGC TAGCCAAAAA GCAGTGCTAC AAGGCACCAC AAATATATTT 
CAGGACTTCC GTCAGCTTCT TGATGAAAAA ACTGGAATAT TTATAAAGAT GAAGGATTTA 
ATCAGTGGTT GGATTCAGAA AGGATCTCAA GACTTCTTCA GGTCACTAGA AGCTCAATTT 
CTAGTGCTTT CTGGAAAAAC TAGCTCATCA AACGATATAG AGGGAAAATC GAGTGACAAA 
ATTCATGCTG GTCTCATTCT TGTATTGGCG CAGCTCTCTG TCTTCATCGA ACAAAAGGTC 
ATCCCGAGAG TTACTGAGGA AATAGCTGCT TCCTTCTCTG GTGGAAATTC CCAAGCCTTT 
GAGAATGGAC CTGCTTTTAT TCCTGGAGAA CTTTGCCGGG TCTTCCACGC AGCCAGCGAG 
AAACTTCTCC AGCATTACAT AGACACAAGA ACACAGAAGG TATCAGTTTT ACTGAGAAAA 
AGGTTCAAGA CACCTAACTG GGTTAAGCAC AAGGAGCCAC GAGAGGTTCA CATGTATGTC 
GATATGTTTC TTCACGAGTT AGAAGAAGTT GGTAAAGAAG TGAAACAAGT TTTACCTCAA 
GGGACTTTCC GTAAGCACAA AAGAACAGAC AGCAACGGAA GTAACACTAC AACCTCATCA 
CGAAGCAATA CCCTCCATAA TGATAAGATG GCACGGTCAA ATTCACAAAG AGCTCGTAGT 
CAGCTTTTCG AGACACATCT TGCAAAACTG TTCAAGCAAA AAGTAGAAAT ATTCACCAAG 
GTTGAATTTA CCCAGGAATC CGTTGTTACA ACAACAGTAA AACTTTGTCT GAAGAGTTTG 
CAAGAATATG TCCGTCTCCA AACGTTTAAC CGGAGCGGGT TCCAGCAGAT TCAGCTAGAC 
ATTCAGTTCT TAAAAGCTCC ATTGAAGGAA GCAGTTGAGG ACGAAGCTGC AATAGACTTC 
TTGCTCGACG AGGTGATCGT TGCGGCTTCG GAGAGATGTC TTGATGTGAT TCCATTGGAG 
CCACCGATCT TGGACAAACT CATACAAGCT AAACTCGCTA AATCAAAGGA GCACAACAAC 
AACACAGTTT CTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190654.1
CDS	87..2504
Translation	MATEAAPMDEKAKRMRDLLSSFYAPDPSISTSGSSINASFDNINSTSFDADQYMDLMIRLFDLGLSDLPAMVDSSELNWVYEIKKSNLEVLLQRHVQMAAEIKNLDTDLQMLVYENYNKFISATDTIKRMKSNIFGMEGNMDQLLQKIMSVQSKSDGVNTSLFEKREHIEKLHRTRNLLRKVQFIYDLPARLQKCIKSEAYGDAVRFYTGAMPILKVYGDTSFQDCRRASEEAIEIIIKNLQTKLFSDSESIQARAEAAVLLKQLDVPVDSLKAKLLEKLEQSLDGLQIKPEEASTLVEDDDSSNDTESNDQHPAKIHEDAVRGFSEAIRAYREIFPDSEERLFKLARALTAMHFEYMELYIKKRVSAADFLGIFRIVWEDVVLMDEVLPEAALSDLSAEAAQVTLKQFVARMFSHLQQDISDTLLKFDINQKEAVEGELLKVVLEASQKAVLQGTTNIFQDFRQLLDEKTGIFIKMKDLISGWIQKGSQDFFRSLEAQFLVLSGKTSSSNDIEGKSSDKIHAGLILVLAQLSVFIEQKVIPRVTEEIAASFSGGNSQAFENGPAFIPGELCRVFHAASEKLLQHYIDTRTQKVSVLLRKRFKTPNWVKHKEPREVHMYVDMFLHELEEVGKEVKQVLPQGTFRKHKRTDSNGSNTTTSSRSNTLHNDKMARSNSQRARSQLFETHLAKLFKQKVEIFTKVEFTQESVVTTTVKLCLKSLQEYVRLQTFNRSGFQQIQLDIQFLKAPLKEAVEDEAAIDFLLDEVIVAASERCLDVIPLEPPILDKLIQAKLAKSKEHNNNTVSS

Target ORF information:

RefSeq Version	NM_001203725.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Vps51/Vps67 family (components of vesicular transport) protein (AT4G02030), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203725.1

ORF Insert Sequence:

ATGGCGACGG AGGCAGCTCC GATGGACGAA AAGGCGAAGA GAATGAGAGA TCTATTGTCG 
AGCTTCTACG CACCGGATCC TTCAATCTCG ACGAGTGGTT CCTCCATCAA CGCCTCTTTC 
GATAACATCA ACAGCACTTC CTTTGATGCT GATCAGTACA TGGATCTCAT GATTCGGCTC 
TTTGATCTTG GTCTAAGTGA TCTTCCTGCA ATGGTTGATA GTTCGGAACT AAATTGGGTT 
TATGAGATCA AAAAGTCGAA TTTGGAGGTG CTTCTGCAAA GACATGTTCA AATGGCTGCT 
GAGATTAAAA ATCTCGACAC AGACTTGCAA ATGCTAGTCT ATGAAAATTA CAACAAGTTT 
ATCAGTGCAA CAGATACAAT CAAAAGGATG AAGAGTAATA TTTTCGGGAT GGAAGGCAAT 
ATGGACCAGC TTCTTCAGAA GATAATGTCA GTACAATCAA AGAGTGATGG GGTCAACACT 
TCTCTTTTCG AAAAGAGAGA ACATATAGAG AAACTGCACC GGACTCGTAA TCTTCTTCGT 
AAAGTTCAGT TCATCTATGA TTTACCTGCA AGACTGCAAA AATGTATCAA GTCAGAAGCC 
TATGGCGATG CTGTCAGGTT CTATACTGGA GCAATGCCAA TTCTCAAGGT ATATGGCGAT 
ACATCATTCC AAGACTGCAG GCGAGCTTCC GAAGAAGCTA TAGAAATTAT CATAAAGAAC 
TTGCAGACGA AGCTATTTTC AGATTCAGAA TCCATACAGG CGAGAGCTGA AGCTGCAGTG 
CTTCTTAAGC AGTTAGATGT CCCTGTTGAT AGCTTAAAGG CTAAACTGTT GGAAAAACTG 
GAACAGTCTC TTGATGGTCT TCAAATAAAG CCTGAGGAGG CAAGTACACT TGTAGAGGAC 
GATGATTCAT CTAACGATAC AGAAAGCAAT GACCAACATC CTGCTAAAAT TCATGAGGAT 
GCCGTACGTG GATTTTCTGA GGCCATACGT GCTTATCGAG AAATATTCCC CGACTCAGAA 
GAAAGACTTT TCAAACTCGC GAGAGCCTTA ACAGCAATGC ACTTTGAGTA CATGGAGCTA 
TATATAAAAA AACGGGTTTC TGCAGCAGAT TTTCTAGGAA TTTTTCGAAT TGTATGGGAA 
GATGTGGTAC TTATGGACGA GGTGCTACCT GAGGCTGCAC TCTCTGATTT ATCTGCAGAG 
GCTGCCCAGG TTACTCTTAA GCAATTTGTT GCGAGAATGT TTTCTCATCT TCAACAGGAT 
ATATCAGATA CTCTCTTGAA ATTCGATATC AACCAAAAGG AAGCAGTTGA AGGGGAACTC 
TTGAAGGTTG TCCTGGAAGC TAGCCAAAAA GCAGTGCTAC AAGGCACCAC AAATATATTT 
CAGGACTTCC GTCAGCTTCT TGATGAAAAA ACTGGAATAT TTATAAAGAT GAAGGATTTA 
ATCAGTGGTT GGATTCAGAA AGGATCTCAA GACTTCTTCA GGTCACTAGA AGCTCAATTT 
CTAGTGCTTT CTGGAAAAAC TAGCTCATCA AACGATATAG AGGGAAAATC GAGTGACAAA 
ATTCATGCTG GTCTCATTCT TGTATTGGCG CAGCTCTCTG TCTTCATCGA ACAAAAGGTC 
ATCCCGAGAG TTACTGAGGA AATAGCTGCT TCCTTCTCTG GTGGAAATTC CCAAGCCTTT 
GAGAATGGAC CTGCTTTTAT TCCTGGAGAA CTTTGCCGGG TCTTCCACGC AGCCAGCGAG 
AAACTTCTCC AGCATTACAT AGACACAAGA ACACAGAAGG TATCAGTTTT ACTGAGAAAA 
AGGTTCAAGA CACCTAACTG GGTTAAGCAC AAGGAGCCAC GAGAGGTTCA CATGTATGTC 
GATATGTTTC TTCACGAGTT AGAAGAAGTT GGTAAAGAAG TGAAACAAGT TTTACCTCAA 
GGGACTTTCC GTAAGCACAA AAGAACAGAC AGCAACGGAA GTAACACTAC AACCTCATCA 
CGAAGCAATA CCCTCCATAA TGATAAGATG GCACGGTCAA ATTCACAAAG AGCTCGTAGT 
CAGCTTTTCG AGACACATCT TGCAAAACTG TTCAAGCAAA AAGTAGAAAT ATTCACCAAG 
GTTGAATTTA CCCAGGAATC CGTTGTTACA ACAACAGTAA AACTTTGTCT GAAGAGTTTG 
CAAGAATATG TCCGTCTCCA AACGTTTAAC CGGAGCGGGT TCCAGCAGAT TCAGCTAGAC 
ATTCAGTTCT TAAAAGCTCC ATTGAAGGAA GCAGTTGAGG ACGAAGCTGC AATAGACTTC 
TTGCTCGACG AGGTGATCGT TGCGGCTTCG GAGAGATGTC TTGATGTGAT TCCATTGGAG 
CCACCGATCT TGGACAAACT CATACAAGCT AAACTCGCTA AATCAAAGGA GCACAACAAC 
AACACAGTTT CTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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AT4G02030 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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