KAT2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	KAT2
Entrez Gene ID	827555
Full Name	potassium channel KAT1-like protein
Synonyms	T9A21.140,T9A21_140,VOLTAGE-GATED POTASSIUM CHANNEL 2,potassium channel in Arabidopsis thaliana 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001341273.1	NP_001329801.1	potassium channel KAT1-like protein
NM_117939.5	NP_193563.3	potassium channel KAT1-like protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC534542	XP_005202558.1
Arabidopsis thaliana (thale cress)	KAT1	NP_199436.1
Homo sapiens (human)	KCNH7	NP_150375.2
Pan troglodytes (chimpanzee)	KCNH7	XP_525954.2
Canis lupus familiaris (dog)	KCNH7	XP_535934.2
Rattus norvegicus (Norway rat)	Kcnh7	NP_571987.1
Gallus gallus (chicken)	KCNH7	XP_422030.3
Danio rerio (zebrafish)	si:dkey-240h6.1	XP_688778.4
Mus musculus (house mouse)	Kcnh7	NP_573470.2
Arabidopsis thaliana (thale cress)	KAT2	NP_193563.3
Oryza sativa (rice)	Os02g0245800	NP_001046422.2
	KCNH7	XP_001097904.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007709	XP_308166.4
Caenorhabditis elegans (roundworm)	unc-103	NP_001122685.1
Xenopus tropicalis (tropical clawed frog)	kcnh7	XP_002933306.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CPK13 reduces stomatal aperture through its inhibition of the guard cell-expressed KAT2 and KAT1 channels.
Title: CPK13, a noncanonical Ca2+-dependent protein kinase, specifically inhibits KAT2 and KAT1 shaker K+ channels and reduces stomatal opening.

The nine-amino acid stretch 312TVRAASEFA320 that composes the first C-linker alpha-helix located just below the pore is a crucial determinant of KAT2 channel activity.
Title: Distinct amino acids in the C-linker domain of the Arabidopsis K+ channel KAT2 determine its subcellular localization and activity at the plasma membrane.

KAT2 contributes to the synthesis of substituted hydroxybenzoylcholines.KAT2 plays the primary role in benzoic acid synthesis.
Title: Peroxisomal ATP-binding cassette transporter COMATOSE and the multifunctional protein abnormal INFLORESCENCE MERISTEM are required for the production of benzoylated metabolites in Arabidopsis seeds.

KAT2 was able to re-locate AtKC1 fused to GFP from endomembranes to the plasma membrane, indicating that heteromeric AtKC1-KAT2 channels are efficiently targeted to the plasma membrane.
Title: AtKC1 is a general modulator of Arabidopsis inward Shaker channel activity.

KAT2 may function downstream of an important WRKY transcription repressor WRKY40, which may link KAT2 with the ABA receptor ABAR/CHLH-mediated signaling.
Title: Arabidopsis 3-ketoacyl-CoA thiolase-2 (KAT2), an enzyme of fatty acid ?-oxidation, is involved in ABA signal transduction.

The following KAT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KAT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15285	NM_117939.5 Latest version!	Arabidopsis thaliana potassium channel KAT1-like protein (KAT2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb39443	NM_001341273.1 Latest version!	Arabidopsis thaliana potassium channel KAT1-like protein (KAT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KAT2 ( NM_117939.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_193563.3 Arabidopsis thaliana potassium channel KAT1-like protein (KAT2), partial mRNA.

Price & Availability↑

CloneID	OAb15285
Clone ID Related Accession (Same CDS sequence)	NM_117939.5
Accession Version	NM_117939.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	potassium channel KAT1-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_117939.4. COMPLETENESS: incomplete on the 5' end.

ATGTCAATCT CTTGTACCAG AAACTTCTTC AAACGATTCT GCGTCGAAGA ATACAATATG 
GATACATTCA AACATAGCAG TTTCCTCTCT GCTGATCTCT TACCATCTCT TGGAGCTAGA 
ATAAACCAAT CAACTAAGCT CCGTAAACAC ATCATCTCTC CTTTCGATCC ACGTTTCAGG 
GGATGGGAGA TGTGGCTGGT CATTCTTGTG ATTTACTCAG CTTGGATTTG TCCATTTGAG 
TTTGCCTTCA TCACCTACAA GAAAGACGCA CTTTTCATCA TTGACAACAT TGTCAATGGA 
TTCTTTGCCA TTGACATCAT TCTCACCTTC TTCGTTGCTT ATCTAGACAG CCACTCATAT 
CTTCTAGTCG ACAAACCTAA GAAAATAGCT ATAAGGTACC TTTCTACTTG GTTCGCCTTT 
GATGTCTGCT CAACAGCTCC ATTCCAGTCA CTGAGTCTTC TGTTCAAATA CAATGGAAGT 
GAAATAGGAT TCAGAGTTCT TAGTATGCTC AGGCTCTGGC GTCTTAGACG AGTTAGCTCG 
CTGTTTGCAA GGCTTGAGAA AGATATCCGC TTCAACTATT TCTGGACACG ATGCACAAAA 
CTCATTTCGG TGACACTGTT TGCAGTACAT TGTGCTGGAT GCTTCGCCTA CCTCATTGCA 
GATCAATATC ATGATCCAAC AAAAACATGG ATTGGAGCTG TCTACCCAAA TTTCAAGGAG 
ACAAGCGTAT GGAGTAGATA TGTTACTGCT CTTTACTGGT CTATTACGAC ATTAACAACA 
ACAGGTTATG GAGACTTACA TGCTGAGAAC CCAAGAGAAA TGTTGTTCTT TGTGTTTTTC 
ATGCTCTTCA ACCTCGGTTT CACATCTTAC CTCATAGGAA ATATGACCAA CCTTGTGGTT 
CACTGGACTA GCCGCACCAG AAACTTTAGA GATACTGTAA GAGCTGCTTC AGAGTTTGCA 
TCAAGAAACC AACTCCCACC AAACATACAA GACCAAATGT TATCACACAT TTGCTTAAAG 
TTTAAAACAG AGGGTCTGAA ACAACAAGAA GCTTTAAATG GTTTGCCGAA AGCAATCCGG 
TCAAGCATTG CAAACTATCT CTTCTTCCCA ATCGTTCAGA ACGTCTACCT ATTTCACGGA 
GTTTCTCGCA ACTTCCTATT TCAGTTGGTT TCAGATATAG ACGCCGAATA TTTCCCACCA 
AGAGAAGATG TAATACTACA GAACGAAGCT CCTACTGACC TCTACATCCT AGTCTCAGGG 
GCAGTGGATT TTACTGTCTA CGTTGGCGAA GAAGATCAGG TTCAAGGCAA GGCGGTTGTT 
GGCGATGCAT TTGGAGAGAT TGGTGTGTTA TGCTATACAC CACAACCTTT CACAGTTAGG 
ACAACAGAAC TATCTCAGAT ACTACGGATA AGCAAAAAAT CTCTAATGAG TGCTATGCGA 
GCTCATGTCG AAGATGGACG AGTCATAATG AACAACCTCT TCATGAAACT TAGAGGGCAA 
CAATCAATAG CAATTGATGA TCCAAATTCA GAACCAGAGT CACTTCTCAA GGAGTGGCTT 
GTTGGTGGTT CAAAGACAGG AGAAGGAAAC GCTAGTGATC AAGGACATGG GCACAAATAT 
TTACAACTGC ATGATTCAGA AAATATTGAT ATGGGGTCAA CTGAATGGAG AGATTCAAGA 
AGATCTGGTT ATGGCGAAAC GAAAAGAGTT CGAGAACATA CGATAGAGAT AGAAGAAGGA 
GAAAAACCAA ATAAAGAGTT TGATGGAAAA GGTTGCTCTG ATGCAGATCT TACCTCATTT 
GAATTTCATT CACAGGAAGC ATATCCATAT TGCAGATCAA ACATCCAAAT CAAACAACAT 
GAAGCTGCAA AACCAAAAGA TAAAAGAGTT ACCATCCACT TGAAGTCCCG GGACAAAGAT 
TTGTCGAAGC TGATAATCCT TCCTGCTTCC ATAGAAGAGT TGCTAAGACT TGCAGGTGAA 
AAATTTGGAT ACAGCTTCAC AAAGGTCACG AATGCGGAAA ACGCAGAAAT AGATGATGAA 
GATGTGATTA GAGATGGTGA TCATTTGTAT ATTCTCATCA ATGAAAACTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_193563.3
CDS	1..2094
Translation	MSISCTRNFFKRFCVEEYNMDTFKHSSFLSADLLPSLGARINQSTKLRKHIISPFDPRFRGWEMWLVILVIYSAWICPFEFAFITYKKDALFIIDNIVNGFFAIDIILTFFVAYLDSHSYLLVDKPKKIAIRYLSTWFAFDVCSTAPFQSLSLLFKYNGSEIGFRVLSMLRLWRLRRVSSLFARLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFAYLIADQYHDPTKTWIGAVYPNFKETSVWSRYVTALYWSITTLTTTGYGDLHAENPREMLFFVFFMLFNLGFTSYLIGNMTNLVVHWTSRTRNFRDTVRAASEFASRNQLPPNIQDQMLSHICLKFKTEGLKQQEALNGLPKAIRSSIANYLFFPIVQNVYLFHGVSRNFLFQLVSDIDAEYFPPREDVILQNEAPTDLYILVSGAVDFTVYVGEEDQVQGKAVVGDAFGEIGVLCYTPQPFTVRTTELSQILRISKKSLMSAMRAHVEDGRVIMNNLFMKLRGQQSIAIDDPNSEPESLLKEWLVGGSKTGEGNASDQGHGHKYLQLHDSENIDMGSTEWRDSRRSGYGETKRVREHTIEIEEGEKPNKEFDGKGCSDADLTSFEFHSQEAYPYCRSNIQIKQHEAAKPKDKRVTIHLKSRDKDLSKLIILPASIEELLRLAGEKFGYSFTKVTNAENAEIDDEDVIRDGDHLYILINENS

Target ORF information:

RefSeq Version	NM_117939.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana potassium channel KAT1-like protein (KAT2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_117939.5

ORF Insert Sequence:

ATGTCAATCT CTTGTACCAG AAACTTCTTC AAACGATTCT GCGTCGAAGA ATACAATATG 
GATACATTCA AACATAGCAG TTTCCTCTCT GCTGATCTCT TACCATCTCT TGGAGCTAGA 
ATAAACCAAT CAACTAAGCT CCGTAAACAC ATCATCTCTC CTTTCGATCC ACGTTTCAGG 
GGATGGGAGA TGTGGCTGGT CATTCTTGTG ATTTACTCAG CTTGGATTTG TCCATTTGAG 
TTTGCCTTCA TCACCTACAA GAAAGACGCA CTTTTCATCA TTGACAACAT TGTCAATGGA 
TTCTTTGCCA TTGACATCAT TCTCACCTTC TTCGTTGCTT ATCTAGACAG CCACTCATAT 
CTTCTAGTCG ACAAACCTAA GAAAATAGCT ATAAGGTACC TTTCTACTTG GTTCGCCTTT 
GATGTCTGCT CAACAGCTCC ATTCCAGTCA CTGAGTCTTC TGTTCAAATA CAATGGAAGT 
GAAATAGGAT TCAGAGTTCT TAGTATGCTC AGGCTCTGGC GTCTTAGACG AGTTAGCTCG 
CTGTTTGCAA GGCTTGAGAA AGATATCCGC TTCAACTATT TCTGGACACG ATGCACAAAA 
CTCATTTCGG TGACACTGTT TGCAGTACAT TGTGCTGGAT GCTTCGCCTA CCTCATTGCA 
GATCAATATC ATGATCCAAC AAAAACATGG ATTGGAGCTG TCTACCCAAA TTTCAAGGAG 
ACAAGCGTAT GGAGTAGATA TGTTACTGCT CTTTACTGGT CTATTACGAC ATTAACAACA 
ACAGGTTATG GAGACTTACA TGCTGAGAAC CCAAGAGAAA TGTTGTTCTT TGTGTTTTTC 
ATGCTCTTCA ACCTCGGTTT CACATCTTAC CTCATAGGAA ATATGACCAA CCTTGTGGTT 
CACTGGACTA GCCGCACCAG AAACTTTAGA GATACTGTAA GAGCTGCTTC AGAGTTTGCA 
TCAAGAAACC AACTCCCACC AAACATACAA GACCAAATGT TATCACACAT TTGCTTAAAG 
TTTAAAACAG AGGGTCTGAA ACAACAAGAA GCTTTAAATG GTTTGCCGAA AGCAATCCGG 
TCAAGCATTG CAAACTATCT CTTCTTCCCA ATCGTTCAGA ACGTCTACCT ATTTCACGGA 
GTTTCTCGCA ACTTCCTATT TCAGTTGGTT TCAGATATAG ACGCCGAATA TTTCCCACCA 
AGAGAAGATG TAATACTACA GAACGAAGCT CCTACTGACC TCTACATCCT AGTCTCAGGG 
GCAGTGGATT TTACTGTCTA CGTTGGCGAA GAAGATCAGG TTCAAGGCAA GGCGGTTGTT 
GGCGATGCAT TTGGAGAGAT TGGTGTGTTA TGCTATACAC CACAACCTTT CACAGTTAGG 
ACAACAGAAC TATCTCAGAT ACTACGGATA AGCAAAAAAT CTCTAATGAG TGCTATGCGA 
GCTCATGTCG AAGATGGACG AGTCATAATG AACAACCTCT TCATGAAACT TAGAGGGCAA 
CAATCAATAG CAATTGATGA TCCAAATTCA GAACCAGAGT CACTTCTCAA GGAGTGGCTT 
GTTGGTGGTT CAAAGACAGG AGAAGGAAAC GCTAGTGATC AAGGACATGG GCACAAATAT 
TTACAACTGC ATGATTCAGA AAATATTGAT ATGGGGTCAA CTGAATGGAG AGATTCAAGA 
AGATCTGGTT ATGGCGAAAC GAAAAGAGTT CGAGAACATA CGATAGAGAT AGAAGAAGGA 
GAAAAACCAA ATAAAGAGTT TGATGGAAAA GGTTGCTCTG ATGCAGATCT TACCTCATTT 
GAATTTCATT CACAGGAAGC ATATCCATAT TGCAGATCAA ACATCCAAAT CAAACAACAT 
GAAGCTGCAA AACCAAAAGA TAAAAGAGTT ACCATCCACT TGAAGTCCCG GGACAAAGAT 
TTGTCGAAGC TGATAATCCT TCCTGCTTCC ATAGAAGAGT TGCTAAGACT TGCAGGTGAA 
AAATTTGGAT ACAGCTTCAC AAAGGTCACG AATGCGGAAA ACGCAGAAAT AGATGATGAA 
GATGTGATTA GAGATGGTGA TCATTTGTAT ATTCTCATCA ATGAAAACTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KAT2 ( NM_001341273.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001329801.1 Arabidopsis thaliana potassium channel KAT1-like protein (KAT2), mRNA.

Price & Availability↑

CloneID	OAb39443
Clone ID Related Accession (Same CDS sequence)	NM_001341273.1
Accession Version	NM_001341273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	potassium channel KAT1-like protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGTTGAAGA GAAAGCACCT CAACACAAGA CCAATGTCAA TCTCTTGTAC CAGAAACTTC 
TTCAAACGAT TCTGCGTCGA AGAATACAAT ATGGATACAT TCAAACATAG CAGTTTCCTC 
TCTGCTGATC TCTTACCATC TCTTGGAGCT AGAATAAACC AATCAACTAA GCTCCGTAAA 
CACATCATCT CTCCTTTCGA TCCACGTTTC AGGGGATGGG AGATGTGGCT GGTCATTCTT 
GTGATTTACT CAGCTTGGAT TTGTCCATTT GAGTTTGCCT TCATCACCTA CAAGAAAGAC 
GCACTTTTCA TCATTGACAA CATTGTCAAT GGATTCTTTG CCATTGACAT CATTCTCACC 
TTCTTCGTTG CTTATCTAGA CAGCCACTCA TATCTTCTAG TCGACAAACC TAAGAAAATA 
GCTATAAGGT ACCTTTCTAC TTGGTTCGCC TTTGATGTCT GCTCAACAGC TCCATTCCAG 
TCACTGAGTC TTCTGTTCAA ATACAATGGA AGTGAAATAG GATTCAGAGT TCTTAGTATG 
CTCAGGCTCT GGCGTCTTAG ACGAGTTAGC TCGCTGTTTG CAAGGCTTGA GAAAGATATC 
CGCTTCAACT ATTTCTGGAC ACGATGCACA AAACTCATTT CGGTGACACT GTTTGCAGTA 
CATTGTGCTG GATGCTTCGC CTACCTCATT GCAGATCAAT ATCATGATCC AACAAAAACA 
TGGATTGGAG CTGTCTACCC AAATTTCAAG GAGACAAGCG TATGGAGTAG ATATGTTACT 
GCTCTTTACT GGTCTATTAC GACATTAACA ACAACAGGTT ATGGAGACTT ACATGCTGAG 
AACCCAAGAG AAATGTTGTT CTTTGTGTTT TTCATGCTCT TCAACCTCGG TTTCACATCT 
TACCTCATAG GAAATATGAC CAACCTTGTG GTTCACTGGA CTAGCCGCAC CAGAAACTTT 
AGAGATACTG TAAGAGCTGC TTCAGAGTTT GCATCAAGAA ACCAACTCCC ACCAAACATA 
CAAGACCAAA TGTTATCACA CATTTGCTTA AAGTTTAAAA CAGAGGGTCT GAAACAACAA 
GAAGCTTTAA ATGGTTTGCC GAAAGCAATC CGGTCAAGCA TTGCAAACTA TCTCTTCTTC 
CCAATCGTTC AGAACGTCTA CCTATTTCAC GGAGTTTCTC GCAACTTCCT ATTTCAGTTG 
GTTTCAGATA TAGACGCCGA ATATTTCCCA CCAAGAGAAG ATGTAATACT ACAGAACGAA 
GCTCCTACTG ACCTCTACAT CCTAGTCTCA GGGGCAGTGG ATTTTACTGT CTACGTTGGC 
GAAGAAGATC AGGTTCAAGG CAAGGCGGTT GTTGGCGATG CATTTGGAGA GATTGGTGTG 
TTATGCTATA CACCACAACC TTTCACAGTT AGGACAACAG AACTATCTCA GATACTACGG 
ATAAGCAAAA AATCTCTAAT GAGTGCTATG CGAGCTCATG TCGAAGATGG ACGAGTCATA 
ATGAACAACC TCTTCATGAA ACTTAGAGGG CAACAATCAA TAGCAATTGA TGATCCAAAT 
TCAGAACCAG AGTCACTTCT CAAGGAGTGG CTTGTTGGTG GTTCAAAGAC AGGAGAAGGA 
AACGCTAGTG ATCAAGGACA TGGGCACAAA TATTTACAAC TGCATGATTC AGAAAATATT 
GATATGGGGT CAACTGAATG GAGAGATTCA AGAAGATCTG GTTATGGCGA AACGAAAAGA 
GTTCGAGAAC ATACGATAGA GATAGAAGAA GGAGAAAAAC CAAATAAAGA GTTTGATGGA 
AAAGGTTGCT CTGATGCAGA TCTTACCTCA TTTGAATTTC ATTCACAGGA AGCATATCCA 
TATTGCAGAT CAAACATCCA AATCAAACAA CATGAAGCTG CAAAACCAAA AGATAAAAGA 
GTTACCATCC ACTTGAAGTC CCGGGACAAA GATTTGTCGA AGCTGATAAT CCTTCCTGCT 
TCCATAGAAG AGTTGCTAAG ACTTGCAGGT GAAAAATTTG GATACAGCTT CACAAAGGTC 
ACGAATGCGG AAAACGCAGA AATAGATGAT GAAGATGTGA TTAGAGATGG TGATCATTTG 
TATATTCTCA TCAATGAAAA CTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001329801.1
CDS	592..2718
Translation	MLKRKHLNTRPMSISCTRNFFKRFCVEEYNMDTFKHSSFLSADLLPSLGARINQSTKLRKHIISPFDPRFRGWEMWLVILVIYSAWICPFEFAFITYKKDALFIIDNIVNGFFAIDIILTFFVAYLDSHSYLLVDKPKKIAIRYLSTWFAFDVCSTAPFQSLSLLFKYNGSEIGFRVLSMLRLWRLRRVSSLFARLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFAYLIADQYHDPTKTWIGAVYPNFKETSVWSRYVTALYWSITTLTTTGYGDLHAENPREMLFFVFFMLFNLGFTSYLIGNMTNLVVHWTSRTRNFRDTVRAASEFASRNQLPPNIQDQMLSHICLKFKTEGLKQQEALNGLPKAIRSSIANYLFFPIVQNVYLFHGVSRNFLFQLVSDIDAEYFPPREDVILQNEAPTDLYILVSGAVDFTVYVGEEDQVQGKAVVGDAFGEIGVLCYTPQPFTVRTTELSQILRISKKSLMSAMRAHVEDGRVIMNNLFMKLRGQQSIAIDDPNSEPESLLKEWLVGGSKTGEGNASDQGHGHKYLQLHDSENIDMGSTEWRDSRRSGYGETKRVREHTIEIEEGEKPNKEFDGKGCSDADLTSFEFHSQEAYPYCRSNIQIKQHEAAKPKDKRVTIHLKSRDKDLSKLIILPASIEELLRLAGEKFGYSFTKVTNAENAEIDDEDVIRDGDHLYILINENS

Target ORF information:

RefSeq Version	NM_001341273.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana potassium channel KAT1-like protein (KAT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341273.1

ORF Insert Sequence:

ATGTTGAAGA GAAAGCACCT CAACACAAGA CCAATGTCAA TCTCTTGTAC CAGAAACTTC 
TTCAAACGAT TCTGCGTCGA AGAATACAAT ATGGATACAT TCAAACATAG CAGTTTCCTC 
TCTGCTGATC TCTTACCATC TCTTGGAGCT AGAATAAACC AATCAACTAA GCTCCGTAAA 
CACATCATCT CTCCTTTCGA TCCACGTTTC AGGGGATGGG AGATGTGGCT GGTCATTCTT 
GTGATTTACT CAGCTTGGAT TTGTCCATTT GAGTTTGCCT TCATCACCTA CAAGAAAGAC 
GCACTTTTCA TCATTGACAA CATTGTCAAT GGATTCTTTG CCATTGACAT CATTCTCACC 
TTCTTCGTTG CTTATCTAGA CAGCCACTCA TATCTTCTAG TCGACAAACC TAAGAAAATA 
GCTATAAGGT ACCTTTCTAC TTGGTTCGCC TTTGATGTCT GCTCAACAGC TCCATTCCAG 
TCACTGAGTC TTCTGTTCAA ATACAATGGA AGTGAAATAG GATTCAGAGT TCTTAGTATG 
CTCAGGCTCT GGCGTCTTAG ACGAGTTAGC TCGCTGTTTG CAAGGCTTGA GAAAGATATC 
CGCTTCAACT ATTTCTGGAC ACGATGCACA AAACTCATTT CGGTGACACT GTTTGCAGTA 
CATTGTGCTG GATGCTTCGC CTACCTCATT GCAGATCAAT ATCATGATCC AACAAAAACA 
TGGATTGGAG CTGTCTACCC AAATTTCAAG GAGACAAGCG TATGGAGTAG ATATGTTACT 
GCTCTTTACT GGTCTATTAC GACATTAACA ACAACAGGTT ATGGAGACTT ACATGCTGAG 
AACCCAAGAG AAATGTTGTT CTTTGTGTTT TTCATGCTCT TCAACCTCGG TTTCACATCT 
TACCTCATAG GAAATATGAC CAACCTTGTG GTTCACTGGA CTAGCCGCAC CAGAAACTTT 
AGAGATACTG TAAGAGCTGC TTCAGAGTTT GCATCAAGAA ACCAACTCCC ACCAAACATA 
CAAGACCAAA TGTTATCACA CATTTGCTTA AAGTTTAAAA CAGAGGGTCT GAAACAACAA 
GAAGCTTTAA ATGGTTTGCC GAAAGCAATC CGGTCAAGCA TTGCAAACTA TCTCTTCTTC 
CCAATCGTTC AGAACGTCTA CCTATTTCAC GGAGTTTCTC GCAACTTCCT ATTTCAGTTG 
GTTTCAGATA TAGACGCCGA ATATTTCCCA CCAAGAGAAG ATGTAATACT ACAGAACGAA 
GCTCCTACTG ACCTCTACAT CCTAGTCTCA GGGGCAGTGG ATTTTACTGT CTACGTTGGC 
GAAGAAGATC AGGTTCAAGG CAAGGCGGTT GTTGGCGATG CATTTGGAGA GATTGGTGTG 
TTATGCTATA CACCACAACC TTTCACAGTT AGGACAACAG AACTATCTCA GATACTACGG 
ATAAGCAAAA AATCTCTAAT GAGTGCTATG CGAGCTCATG TCGAAGATGG ACGAGTCATA 
ATGAACAACC TCTTCATGAA ACTTAGAGGG CAACAATCAA TAGCAATTGA TGATCCAAAT 
TCAGAACCAG AGTCACTTCT CAAGGAGTGG CTTGTTGGTG GTTCAAAGAC AGGAGAAGGA 
AACGCTAGTG ATCAAGGACA TGGGCACAAA TATTTACAAC TGCATGATTC AGAAAATATT 
GATATGGGGT CAACTGAATG GAGAGATTCA AGAAGATCTG GTTATGGCGA AACGAAAAGA 
GTTCGAGAAC ATACGATAGA GATAGAAGAA GGAGAAAAAC CAAATAAAGA GTTTGATGGA 
AAAGGTTGCT CTGATGCAGA TCTTACCTCA TTTGAATTTC ATTCACAGGA AGCATATCCA 
TATTGCAGAT CAAACATCCA AATCAAACAA CATGAAGCTG CAAAACCAAA AGATAAAAGA 
GTTACCATCC ACTTGAAGTC CCGGGACAAA GATTTGTCGA AGCTGATAAT CCTTCCTGCT 
TCCATAGAAG AGTTGCTAAG ACTTGCAGGT GAAAAATTTG GATACAGCTT CACAAAGGTC 
ACGAATGCGG AAAACGCAGA AATAGATGAT GAAGATGTGA TTAGAGATGG TGATCATTTG 
TATATTCTCA TCAATGAAAA CTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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KAT2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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