ACX1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACX1
Entrez Gene ID	827381
Full Name	acyl-CoA oxidase 1
Synonyms	ATACX1,DL4405C,FCAALL.119,acyl-CoA oxidase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_117778.8	NP_567513.4	acyl-CoA oxidase 1
NM_001203815.1	NP_001190744.1	acyl-CoA oxidase 1

Pathways from BioSystems

Homologies

	ACOX1	XP_001102134.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011798	XP_320718.4
Caenorhabditis elegans (roundworm)	F08A8.3	NP_493263.1
Arabidopsis thaliana (thale cress)	ACX5	NP_181112.1
Bos taurus (cattle)	ACOX1	NP_001030366.1
Mus musculus (house mouse)	Acox1	NP_056544.2
Rattus norvegicus (Norway rat)	Acox1	NP_059036.1
Caenorhabditis elegans (roundworm)	F59F4.1	NP_510603.1
Arabidopsis thaliana (thale cress)	ACX1	NP_567513.4
Homo sapiens (human)	ACOX1	NP_009223.2
Pan troglodytes (chimpanzee)	ACOX1	XP_001149084.1
Gallus gallus (chicken)	ACOX1	NP_001006205.1
Oryza sativa (rice)	Os06g0103500	NP_001056542.1
Canis lupus familiaris (dog)	ACOX1	XP_003435209.1
Caenorhabditis elegans (roundworm)	acox-1	NP_001021089.1
Danio rerio (zebrafish)	acox1	NP_001005933.1
Drosophila melanogaster (fruit fly)	CG5009	NP_611264.2
Caenorhabditis elegans (roundworm)	F08A8.2	NP_493262.2
Caenorhabditis elegans (roundworm)	F08A8.4	NP_493264.1
Caenorhabditis elegans (roundworm)	C48B4.1	NP_499119.1
Xenopus tropicalis (tropical clawed frog)	acox1	NP_001015762.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that acx3acx4 Columbia (Col) and acx1acx3acx4(Col) mutants are viable and that enzyme activity in these mutants is significantly reduced on a range of substrates compared to wild type.
Title: Peroxisomal Acyl-CoA oxidase 4 activity differs between Arabidopsis accessions.

The effects of mutations in ACX1 and ACX5 in A. thaliana on jasmonic acid synthesis are reported.
Title: Functional diversification of acyl-coenzyme A oxidases in jasmonic acid biosynthesis and action.

x-ray crystallography of two peroxisomal acyl-CoA oxidases, ACX1 and ACX4, from Arabidopsis thaliana
Title: Expression, purification and crystallization of two peroxisomal acyl-CoA oxidases from Arabidopsis thaliana.

The following ACX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15129	NM_117778.8 Latest version!	Arabidopsis thaliana acyl-CoA oxidase 1 (ACX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb29575	NM_001203815.1 Latest version!	Arabidopsis thaliana acyl-CoA oxidase 1 (ACX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACX1 ( NM_117778.8 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567513.4 Arabidopsis thaliana acyl-CoA oxidase 1 (ACX1), mRNA.

Price & Availability↑

CloneID	OAb15129
Clone ID Related Accession (Same CDS sequence)	NM_117778.8
Accession Version	NM_117778.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	acyl-CoA oxidase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_117778.7.

ATGGAAGGAA TTGATCACCT CGCCGATGAG AGAAACAAAG CAGAGTTCGA CGTTGAGGAT 
ATGAAGATCG TCTGGGCTGG TTCCCGCCAC GCTTTTGAGG TTTCCGATCG AATTGCCCGC 
CTTGTCGCCA GCGATCCGGT ATTTGAGAAA AGCAATCGAG CTCGGTTGAG TAGGAAGGAG 
CTGTTTAAGA GTACGTTGAG AAAATGTGCC CATGCGTTTA AAAGGATTAT CGAGCTTCGT 
CTCAATGAGG AAGAAGCAGG AAGATTGAGG CACTTTATCG ACCAGCCTGC CTATGTGGAT 
CTGCACTGGG GAATGTTTGT GCCTGCTATT AAGGGGCAGG GTACAGAGGA GCAGCAGAAG 
AAGTGGTTGT CGCTGGCCAA TAAGATGCAG ATTATTGGGT GTTATGCACA GACTGAGCTT 
GGTCATGGCT CAAATGTTCA AGGACTTGAG ACAACTGCCA CATTTGATCC CAAGACTGAT 
GAGTTTGTAA TTCACACTCC AACTCAGACT GCATCCAAAT GGTGGCCTGG TGGTTTGGGA 
AAAGTTTCTA CTCATGCTGT TGTTTACGCT CGTCTCATAA CTAACGGAAA AGACTACGGT 
ATCCATGGAT TCATCGTGCA ACTGCGAAGC TTAGAAGATC ATTCTCCTCT TCCGAATATA 
ACTGTTGGTG ATATCGGGAC AAAGATGGGA AATGGAGCAT ATAATTCAAT GGACAACGGG 
TTTCTTATGT TTGATCATGT TCGCATTCCT AGAGATCAAA TGCTCATGAG GCTGTCAAAA 
GTTACAAGAG AAGGAGAATA TGTTCCATCG GATGTTCCAA AGCAGCTGGT ATATGGTACT 
ATGGTGTATG TGAGACAAAC AATTGTGGCT GATGCTTCCA ATGCACTATC TCGAGCAGTT 
TGCATAGCTA CAAGATACAG TGCAGTGCGG AGGCAATTTG GCGCACATAA TGGTGGCATT 
GAGACACAGG TGATTGATTA TAAAACTCAG CAGAACAGGC TATTTCCTCT GCTAGCATCT 
GCATATGCAT TTCGATTTGT TGGAGAGTGG CTAAAATGGC TGTACACGGA TGTAACTGAA 
AGACTGGCGG CTAGTGATTT CGCAACTTTG CCTGAGGCTC ATGCATGCAC TGCAGGATTG 
AAGTCTCTCA CCACCACAGC CACTGCGGAT GGCATTGAAG AATGTCGTAA GTTATGTGGT 
GGACATGGAT ACTTGTGGTG CAGTGGGCTC CCCGAGCTGT TTGCTGTATA TGTTCCTGCC 
TGCACATACG AAGGAGACAA TGTTGTGCTG CAATTACAGG TTGCTCGATT CCTCATGAAG 
ACAGTCGCCC AGCTGGGATC TGGAAAGGTT CCTGTTGGCA CAACTGCTTA TATGGGCCGG 
GCAGCACATC TTTTGCAATG TCGTTCTGGT GTTCAAAAGG CTGAGGATTG GTTAAACCCT 
GATGTTGTAC TGGAAGCTTT CGAAGCTAGG GCTCTCAGAA TGGCTGTTAC GTGTGCCAAA 
AATCTCAGCA AGTTTGAGAA TCAGGAACAA GGATTCCAAG AGCTCTTGGC TGATTTGGTT 
GAGGCCGCTA TTGCTCATTG CCAATTGATT GTTGTTTCCA AGTTCATAGC GAAACTGGAG 
CAAGACATAG GTGGCAAAGG AGTGAAGAAA CAGCTGAATA ATCTGTGTTA CATTTATGCT 
CTTTATCTCC TCCACAAACA TCTCGGCGAT TTCCTCTCCA CTAACTGCAT CACTCCCAAA 
CAAGCCTCTC TTGCTAACGA CCAGCTCCGT TCCTTATACA CTCAGGTCCG GCCTAATGCG 
GTTGCACTTG TGGACGCCTT CAATTACACC GACCATTACT TGAACTCGGT TCTTGGCCGT 
TACGACGGTA ATGTGTACCC AAAGCTCTTT GAGGAAGCGT TGAAGGATCC ATTGAACGAC 
TCGGTGGTTC CTGATGGGTA CCAAGAATAC CTTCGACCTG TGCTTCAGCA GCAACTTCGT 
ACCGCTAGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567513.4
CDS	206..2200
Translation	MEGIDHLADERNKAEFDVEDMKIVWAGSRHAFEVSDRIARLVASDPVFEKSNRARLSRKELFKSTLRKCAHAFKRIIELRLNEEEAGRLRHFIDQPAYVDLHWGMFVPAIKGQGTEEQQKKWLSLANKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHTPTQTASKWWPGGLGKVSTHAVVYARLITNGKDYGIHGFIVQLRSLEDHSPLPNITVGDIGTKMGNGAYNSMDNGFLMFDHVRIPRDQMLMRLSKVTREGEYVPSDVPKQLVYGTMVYVRQTIVADASNALSRAVCIATRYSAVRRQFGAHNGGIETQVIDYKTQQNRLFPLLASAYAFRFVGEWLKWLYTDVTERLAASDFATLPEAHACTAGLKSLTTTATADGIEECRKLCGGHGYLWCSGLPELFAVYVPACTYEGDNVVLQLQVARFLMKTVAQLGSGKVPVGTTAYMGRAAHLLQCRSGVQKAEDWLNPDVVLEAFEARALRMAVTCAKNLSKFENQEQGFQELLADLVEAAIAHCQLIVVSKFIAKLEQDIGGKGVKKQLNNLCYIYALYLLHKHLGDFLSTNCITPKQASLANDQLRSLYTQVRPNAVALVDAFNYTDHYLNSVLGRYDGNVYPKLFEEALKDPLNDSVVPDGYQEYLRPVLQQQLRTARL

Target ORF information:

RefSeq Version	NM_117778.8
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana acyl-CoA oxidase 1 (ACX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_117778.8

ORF Insert Sequence:

ATGGAAGGAA TTGATCACCT CGCCGATGAG AGAAACAAAG CAGAGTTCGA CGTTGAGGAT 
ATGAAGATCG TCTGGGCTGG TTCCCGCCAC GCTTTTGAGG TTTCCGATCG AATTGCCCGC 
CTTGTCGCCA GCGATCCGGT ATTTGAGAAA AGCAATCGAG CTCGGTTGAG TAGGAAGGAG 
CTGTTTAAGA GTACGTTGAG AAAATGTGCC CATGCGTTTA AAAGGATTAT CGAGCTTCGT 
CTCAATGAGG AAGAAGCAGG AAGATTGAGG CACTTTATCG ACCAGCCTGC CTATGTGGAT 
CTGCACTGGG GAATGTTTGT GCCTGCTATT AAGGGGCAGG GTACAGAGGA GCAGCAGAAG 
AAGTGGTTGT CGCTGGCCAA TAAGATGCAG ATTATTGGGT GTTATGCACA GACTGAGCTT 
GGTCATGGCT CAAATGTTCA AGGACTTGAG ACAACTGCCA CATTTGATCC CAAGACTGAT 
GAGTTTGTAA TTCACACTCC AACTCAGACT GCATCCAAAT GGTGGCCTGG TGGTTTGGGA 
AAAGTTTCTA CTCATGCTGT TGTTTACGCT CGTCTCATAA CTAACGGAAA AGACTACGGT 
ATCCATGGAT TCATCGTGCA ACTGCGAAGC TTAGAAGATC ATTCTCCTCT TCCGAATATA 
ACTGTTGGTG ATATCGGGAC AAAGATGGGA AATGGAGCAT ATAATTCAAT GGACAACGGG 
TTTCTTATGT TTGATCATGT TCGCATTCCT AGAGATCAAA TGCTCATGAG GCTGTCAAAA 
GTTACAAGAG AAGGAGAATA TGTTCCATCG GATGTTCCAA AGCAGCTGGT ATATGGTACT 
ATGGTGTATG TGAGACAAAC AATTGTGGCT GATGCTTCCA ATGCACTATC TCGAGCAGTT 
TGCATAGCTA CAAGATACAG TGCAGTGCGG AGGCAATTTG GCGCACATAA TGGTGGCATT 
GAGACACAGG TGATTGATTA TAAAACTCAG CAGAACAGGC TATTTCCTCT GCTAGCATCT 
GCATATGCAT TTCGATTTGT TGGAGAGTGG CTAAAATGGC TGTACACGGA TGTAACTGAA 
AGACTGGCGG CTAGTGATTT CGCAACTTTG CCTGAGGCTC ATGCATGCAC TGCAGGATTG 
AAGTCTCTCA CCACCACAGC CACTGCGGAT GGCATTGAAG AATGTCGTAA GTTATGTGGT 
GGACATGGAT ACTTGTGGTG CAGTGGGCTC CCCGAGCTGT TTGCTGTATA TGTTCCTGCC 
TGCACATACG AAGGAGACAA TGTTGTGCTG CAATTACAGG TTGCTCGATT CCTCATGAAG 
ACAGTCGCCC AGCTGGGATC TGGAAAGGTT CCTGTTGGCA CAACTGCTTA TATGGGCCGG 
GCAGCACATC TTTTGCAATG TCGTTCTGGT GTTCAAAAGG CTGAGGATTG GTTAAACCCT 
GATGTTGTAC TGGAAGCTTT CGAAGCTAGG GCTCTCAGAA TGGCTGTTAC GTGTGCCAAA 
AATCTCAGCA AGTTTGAGAA TCAGGAACAA GGATTCCAAG AGCTCTTGGC TGATTTGGTT 
GAGGCCGCTA TTGCTCATTG CCAATTGATT GTTGTTTCCA AGTTCATAGC GAAACTGGAG 
CAAGACATAG GTGGCAAAGG AGTGAAGAAA CAGCTGAATA ATCTGTGTTA CATTTATGCT 
CTTTATCTCC TCCACAAACA TCTCGGCGAT TTCCTCTCCA CTAACTGCAT CACTCCCAAA 
CAAGCCTCTC TTGCTAACGA CCAGCTCCGT TCCTTATACA CTCAGGTCCG GCCTAATGCG 
GTTGCACTTG TGGACGCCTT CAATTACACC GACCATTACT TGAACTCGGT TCTTGGCCGT 
TACGACGGTA ATGTGTACCC AAAGCTCTTT GAGGAAGCGT TGAAGGATCC ATTGAACGAC 
TCGGTGGTTC CTGATGGGTA CCAAGAATAC CTTCGACCTG TGCTTCAGCA GCAACTTCGT 
ACCGCTAGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACX1 ( NM_001203815.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190744.1 Arabidopsis thaliana acyl-CoA oxidase 1 (ACX1), mRNA.

Price & Availability↑

CloneID	OAb29575
Clone ID Related Accession (Same CDS sequence)	NM_001203815.1
Accession Version	NM_001203815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	acyl-CoA oxidase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGAAGGAA TTGATCACCT CGCCGATGAG AGAAACAAAG CAGAGTTCGA CGTTGAGGAT 
ATGAAGATCG TCTGGGCTGG TTCCCGCCAC GCTTTTGAGG TATTTGAGAA AAGCAATCGA 
GCTCGGTTGA GTAGGAAGGA GCTGTTTAAG AGTACGTTGA GAAAATGTGC CCATGCGTTT 
AAAAGGATTA TCGAGCTTCG TCTCAATGAG GAAGAAGCAG GAAGATTGAG GCACTTTATC 
GACCAGCCTG CCTATGTGGA TCTGCACTGG GGAATGTTTG TGCCTGCTAT TAAGGGGCAG 
GGTACAGAGG AGCAGCAGAA GAAGTGGTTG TCGCTGGCCA ATAAGATGCA GATTATTGGG 
TGTTATGCAC AGACTGAGCT TGGTCATGGC TCAAATGTTC AAGGACTTGA GACAACTGCC 
ACATTTGATC CCAAGACTGA TGAGTTTGTA ATTCACACTC CAACTCAGAC TGCATCCAAA 
TGGTGGCCTG GTGGTTTGGG AAAAGTTTCT ACTCATGCTG TTGTTTACGC TCGTCTCATA 
ACTAACGGAA AAGACTACGG TATCCATGGA TTCATCGTGC AACTGCGAAG CTTAGAAGAT 
CATTCTCCTC TTCCGAATAT AACTGTTGGT GATATCGGGA CAAAGATGGG AAATGGAGCA 
TATAATTCAA TGGACAACGG GTTTCTTATG TTTGATCATG TTCGCATTCC TAGAGATCAA 
ATGCTCATGA GGCTGTCAAA AGTTACAAGA GAAGGAGAAT ATGTTCCATC GGATGTTCCA 
AAGCAGCTGG TATATGGTAC TATGGTGTAT GTGAGACAAA CAATTGTGGC TGATGCTTCC 
AATGCACTAT CTCGAGCAGT TTGCATAGCT ACAAGATACA GTGCAGTGCG GAGGCAATTT 
GGCGCACATA ATGGTGGCAT TGAGACACAG GTGATTGATT ATAAAACTCA GCAGAACAGG 
CTATTTCCTC TGCTAGCATC TGCATATGCA TTTCGATTTG TTGGAGAGTG GCTAAAATGG 
CTGTACACGG ATGTAACTGA AAGACTGGCG GCTAGTGATT TCGCAACTTT GCCTGAGGCT 
CATGCATGCA CTGCAGGATT GAAGTCTCTC ACCACCACAG CCACTGCGGA TGGCATTGAA 
GAATGTCGTA AGTTATGTGG TGGACATGGA TACTTGTGGT GCAGTGGGCT CCCCGAGCTG 
TTTGCTGTAT ATGTTCCTGC CTGCACATAC GAAGGAGACA ATGTTGTGCT GCAATTACAG 
GTTGCTCGAT TCCTCATGAA GACAGTCGCC CAGCTGGGAT CTGGAAAGGT TCCTGTTGGC 
ACAACTGCTT ATATGGGCCG GGCAGCACAT CTTTTGCAAT GTCGTTCTGG TGTTCAAAAG 
GCTGAGGATT GGTTAAACCC TGATGTTGTA CTGGAAGCTT TCGAAGCTAG GGCTCTCAGA 
ATGGCTGTTA CGTGTGCCAA AAATCTCAGC AAGTTTGAGA ATCAGGAACA AGGATTCCAA 
GAGCTCTTGG CTGATTTGGT TGAGGCCGCT ATTGCTCATT GCCAATTGAT TGTTGTTTCC 
AAGTTCATAG CGAAACTGGA GCAAGACATA GGTGGCAAAG GAGTGAAGAA ACAGCTGAAT 
AATCTGTGTT ACATTTATGC TCTTTATCTC CTCCACAAAC ATCTCGGCGA TTTCCTCTCC 
ACTAACTGCA TCACTCCCAA ACAAGCCTCT CTTGCTAACG ACCAGCTCCG TTCCTTATAC 
ACTCAGGTCC GGCCTAATGC GGTTGCACTT GTGGACGCCT TCAATTACAC CGACCATTAC 
TTGAACTCGG TTCTTGGCCG TTACGACGGT AATGTGTACC CAAAGCTCTT TGAGGAAGCG 
TTGAAGGATC CATTGAACGA CTCGGTGGTT CCTGATGGGT ACCAAGAATA CCTTCGACCT 
GTGCTTCAGC AGCAACTTCG TACCGCTAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190744.1
CDS	207..2162
Translation	MEGIDHLADERNKAEFDVEDMKIVWAGSRHAFEVFEKSNRARLSRKELFKSTLRKCAHAFKRIIELRLNEEEAGRLRHFIDQPAYVDLHWGMFVPAIKGQGTEEQQKKWLSLANKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHTPTQTASKWWPGGLGKVSTHAVVYARLITNGKDYGIHGFIVQLRSLEDHSPLPNITVGDIGTKMGNGAYNSMDNGFLMFDHVRIPRDQMLMRLSKVTREGEYVPSDVPKQLVYGTMVYVRQTIVADASNALSRAVCIATRYSAVRRQFGAHNGGIETQVIDYKTQQNRLFPLLASAYAFRFVGEWLKWLYTDVTERLAASDFATLPEAHACTAGLKSLTTTATADGIEECRKLCGGHGYLWCSGLPELFAVYVPACTYEGDNVVLQLQVARFLMKTVAQLGSGKVPVGTTAYMGRAAHLLQCRSGVQKAEDWLNPDVVLEAFEARALRMAVTCAKNLSKFENQEQGFQELLADLVEAAIAHCQLIVVSKFIAKLEQDIGGKGVKKQLNNLCYIYALYLLHKHLGDFLSTNCITPKQASLANDQLRSLYTQVRPNAVALVDAFNYTDHYLNSVLGRYDGNVYPKLFEEALKDPLNDSVVPDGYQEYLRPVLQQQLRTARL

Target ORF information:

RefSeq Version	NM_001203815.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana acyl-CoA oxidase 1 (ACX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203815.1

ORF Insert Sequence:

ATGGAAGGAA TTGATCACCT CGCCGATGAG AGAAACAAAG CAGAGTTCGA CGTTGAGGAT 
ATGAAGATCG TCTGGGCTGG TTCCCGCCAC GCTTTTGAGG TATTTGAGAA AAGCAATCGA 
GCTCGGTTGA GTAGGAAGGA GCTGTTTAAG AGTACGTTGA GAAAATGTGC CCATGCGTTT 
AAAAGGATTA TCGAGCTTCG TCTCAATGAG GAAGAAGCAG GAAGATTGAG GCACTTTATC 
GACCAGCCTG CCTATGTGGA TCTGCACTGG GGAATGTTTG TGCCTGCTAT TAAGGGGCAG 
GGTACAGAGG AGCAGCAGAA GAAGTGGTTG TCGCTGGCCA ATAAGATGCA GATTATTGGG 
TGTTATGCAC AGACTGAGCT TGGTCATGGC TCAAATGTTC AAGGACTTGA GACAACTGCC 
ACATTTGATC CCAAGACTGA TGAGTTTGTA ATTCACACTC CAACTCAGAC TGCATCCAAA 
TGGTGGCCTG GTGGTTTGGG AAAAGTTTCT ACTCATGCTG TTGTTTACGC TCGTCTCATA 
ACTAACGGAA AAGACTACGG TATCCATGGA TTCATCGTGC AACTGCGAAG CTTAGAAGAT 
CATTCTCCTC TTCCGAATAT AACTGTTGGT GATATCGGGA CAAAGATGGG AAATGGAGCA 
TATAATTCAA TGGACAACGG GTTTCTTATG TTTGATCATG TTCGCATTCC TAGAGATCAA 
ATGCTCATGA GGCTGTCAAA AGTTACAAGA GAAGGAGAAT ATGTTCCATC GGATGTTCCA 
AAGCAGCTGG TATATGGTAC TATGGTGTAT GTGAGACAAA CAATTGTGGC TGATGCTTCC 
AATGCACTAT CTCGAGCAGT TTGCATAGCT ACAAGATACA GTGCAGTGCG GAGGCAATTT 
GGCGCACATA ATGGTGGCAT TGAGACACAG GTGATTGATT ATAAAACTCA GCAGAACAGG 
CTATTTCCTC TGCTAGCATC TGCATATGCA TTTCGATTTG TTGGAGAGTG GCTAAAATGG 
CTGTACACGG ATGTAACTGA AAGACTGGCG GCTAGTGATT TCGCAACTTT GCCTGAGGCT 
CATGCATGCA CTGCAGGATT GAAGTCTCTC ACCACCACAG CCACTGCGGA TGGCATTGAA 
GAATGTCGTA AGTTATGTGG TGGACATGGA TACTTGTGGT GCAGTGGGCT CCCCGAGCTG 
TTTGCTGTAT ATGTTCCTGC CTGCACATAC GAAGGAGACA ATGTTGTGCT GCAATTACAG 
GTTGCTCGAT TCCTCATGAA GACAGTCGCC CAGCTGGGAT CTGGAAAGGT TCCTGTTGGC 
ACAACTGCTT ATATGGGCCG GGCAGCACAT CTTTTGCAAT GTCGTTCTGG TGTTCAAAAG 
GCTGAGGATT GGTTAAACCC TGATGTTGTA CTGGAAGCTT TCGAAGCTAG GGCTCTCAGA 
ATGGCTGTTA CGTGTGCCAA AAATCTCAGC AAGTTTGAGA ATCAGGAACA AGGATTCCAA 
GAGCTCTTGG CTGATTTGGT TGAGGCCGCT ATTGCTCATT GCCAATTGAT TGTTGTTTCC 
AAGTTCATAG CGAAACTGGA GCAAGACATA GGTGGCAAAG GAGTGAAGAA ACAGCTGAAT 
AATCTGTGTT ACATTTATGC TCTTTATCTC CTCCACAAAC ATCTCGGCGA TTTCCTCTCC 
ACTAACTGCA TCACTCCCAA ACAAGCCTCT CTTGCTAACG ACCAGCTCCG TTCCTTATAC 
ACTCAGGTCC GGCCTAATGC GGTTGCACTT GTGGACGCCT TCAATTACAC CGACCATTAC 
TTGAACTCGG TTCTTGGCCG TTACGACGGT AATGTGTACC CAAAGCTCTT TGAGGAAGCG 
TTGAAGGATC CATTGAACGA CTCGGTGGTT CCTGATGGGT ACCAAGAATA CCTTCGACCT 
GTGCTTCAGC AGCAACTTCG TACCGCTAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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ACX1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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