CHX20 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHX20
Entrez Gene ID	824539
Full Name	cation/H+ exchanger 20
Synonyms	ATCHX20,cation/H+ exchanger 20
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_115232.3	NP_190940.1	cation/H+ exchanger 20
NM_001339628.1	NP_001325440.1	cation/H+ exchanger 20

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	CHX20	NP_190940.1
Oryza sativa (rice)	Os05g0113300	NP_001054455.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CHX20 is preferentially and highly expressed in guard cells
Title: Participation of endomembrane cation/H+ exchanger AtCHX20 in osmoregulation of guard cells.

The following CHX20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHX20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb31627	NM_115232.3 Latest version!	Arabidopsis thaliana cation/H+ exchanger 20 (CHX20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAb38314	NM_001339628.1 Latest version!	Arabidopsis thaliana cation/H+ exchanger 20 (CHX20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHX20 ( NM_115232.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_190940.1 Arabidopsis thaliana cation/H+ exchanger 20 (CHX20), mRNA.

Price & Availability↑

CloneID	OAb31627
Clone ID Related Accession (Same CDS sequence)	NM_115232.3
Accession Version	NM_115232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cation/H+ exchanger 20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_115232.2.

ATGCCCTTCA ACATAACCTC CGTGAAAACC TCATCTAACG GAGTATGGCA AGGCGACAAT 
CCTTTAAACT TCGCTTTTCC GTTACTCATC GTCCAAACGG CGTTAATCAT CGCCGTCAGT 
CGCTTCCTCG CCGTCTTATT CAAACCTCTC CGTCAACCCA AAGTCATCGC CGAGATTGTC 
GGAGGGATTT TGTTAGGACC ATCGGCTTTA GGTAGAAACA TGGCGTACAT GGACCGTATA 
TTTCCGAAAT GGAGTATGCC GATACTCGAA TCCGTCGCGA GCATAGGACT TCTCTTCTTC 
CTCTTCCTCG TCGGTCTAGA ACTCGATTTA TCATCGATCC GACGAAGCGG CAAACGCGCT 
TTCGGAATCG CAGTCGCTGG AATTACACTA CCGTTTATCG CCGGCGTCGG AGTCGCGTTT 
GTGATCCGTA ACACTCTCTA CACCGCCGCG GATAAACCAG GTTACGCCGA GTTTCTCGTT 
TTCATGGGAG TCGCACTCTC GATCACAGCT TTTCCGGTAC TTGCGCGTAT TTTAGCAGAG 
CTCAAGCTTT TAACGACTCA GATAGGAGAA ACCGCGATGG CTGCAGCCGC TTTTAACGAT 
GTAGCCGCGT GGATTTTACT CGCTTTAGCG GTTGCGTTAG CGGGTAATGG CGGTGAGGGA 
GGTGGAGAGA AAAAGAGTCC GTTAGTGTCG TTGTGGGTTT TGTTATCGGG CGCTGGGTTT 
GTGGTTTTTA TGTTGGTTGT GATCCGACCC GGAATGAAAT GGGTCGCGAA ACGTGGATCT 
CCTGAAAACG ACGTCGTACG CGAGTCTTAC GTGTGTTTGA CGTTAGCCGG TGTTATGGTT 
TCCGGTTTCG CGACGGATTT AATTGGGATT CATTCGATTT TTGGAGCGTT TGTTTTCGGT 
TTGACTATAC CGAAAGATGG AGAGTTTGGT CAGCGATTGA TTGAACGAAT TGAGGATTTT 
GTTTCCGGTT TACTCTTACC GCTTTATTTC GCTACGAGTG GTTTGAAGAC TGACGTGGCT 
AAGATTAGAG GAGCTGAGTC GTGGGGGATG TTGGGTCTTG TTGTTGTTAC GGCTTGTGCC 
GGGAAGATAG TCGGAACTTT TGTTGTGGCG GTGATGGTTA AAGTTCCGGC GAGAGAGGCG 
TTGACACTTG GTTTCTTGAT GAATACTAAA GGTTTAGTGG AGCTCATTGT ACTCAACATA 
GGCAAGGAGA AAAAGGTACT AAACGACGAG ACGTTTGCAA TACTAGTGCT AATGGCACTC 
TTCACAACGT TCATAACGAC GCCTACTGTA ATGGCCATTT ACAAGCCGGC ACGTGGCACC 
CACCGCAAAC TAAAAGACTT GTCGGCGAGC CAAGACTCCA CCAAGGAAGA GCTTCGCATC 
CTAGCCTGCC TCCACGGCCC AGCCAATGTC TCCTCCCTCA TCTCTCTCGT CGAGTCCATC 
CGAACCACCA AGATACTACG GCTAAAGCTG TTTGTGATGC ATCTGATGGA ACTAACGGAA 
CGGTCTTCGT CAATCATAAT GGTGCAAAGA GCCCGTAAAA ACGGACTTCC TTTCGTTCAC 
CGTTACCGTC ATGGTGAGCG TCACAGCAAC GTCATAGGAG GCTTCGAAGC CTATCGTCAA 
CTAGGCCGGG TCGCAGTCCG GCCCATCACC GCAGTCTCTC CATTACCCAC AATGCACGAA 
GACATTTGCC ACATGGCAGA TACCAAGAGG GTCACAATGA TCATTTTACC TTTCCACAAA 
CGATGGAACG CTGATCATGG TCATAGCCAC CACCACCAAG ACGGAGGAGG AGATGGAAAC 
GTACCGGAAA ACGTTGGTCA TGGTTGGCGA TTGGTTAACC AAAGGGTTTT GAAGAATGCG 
CCGTGTTCGG TGGCGGTTCT TGTAGACCGT GGACTTGGGT CCATTGAGGC CCAAACTTTG 
AGCTTAGATG GGTCGAATGT GGTTGAAAGG GTTTGTGTGA TTTTCTTTGG TGGGCCTGAT 
GACCGTGAGT CTATAGAGCT CGGCGGGAGA ATGGCTGAGC ATCCGGCCGT TAAAGTTACC 
GTTATTAGGT TTTTGGTAAG AGAAACGTTG AGGAGTACCG CCGTCACTTT ACGACCGGCA 
CCGTCTAAAG GCAAGGAGAA GAACTATGCC TTTTTAACAA CCAACGTGGA TCCAGAAAAA 
GAAAAGGAAT TAGACGAAGG GGCATTGGAA GACTTCAAGA GCAAATGGAA AGAAATGGTG 
GAGTACAAAG AAAAGGAACC AAACAACATC ATTGAAGAAA TACTGTCAAT AGGACAGAGT 
AAAGACTTTG ATCTAATAGT GGTTGGAAGA GGGAGGATAC CGTCGGCCGA GGTGGCGGCA 
TTAGCTGAGC GTCAAGCTGA ACATCCTGAG TTAGGTCCTA TCGGAGACGT GCTCGCCTCT 
TCGATCAACC ACATCATTCC ATCAATCCTT GTGGTTCAAC AACACAACAA AGCTCATGTA 
GAGGATATTA CGGTTTCCAA AATTGTTAGT GAGTCTTCTC TAAGTATTAA CGGAGACACA 
AATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_190940.1
CDS	200..2728
Translation	MPFNITSVKTSSNGVWQGDNPLNFAFPLLIVQTALIIAVSRFLAVLFKPLRQPKVIAEIVGGILLGPSALGRNMAYMDRIFPKWSMPILESVASIGLLFFLFLVGLELDLSSIRRSGKRAFGIAVAGITLPFIAGVGVAFVIRNTLYTAADKPGYAEFLVFMGVALSITAFPVLARILAELKLLTTQIGETAMAAAAFNDVAAWILLALAVALAGNGGEGGGEKKSPLVSLWVLLSGAGFVVFMLVVIRPGMKWVAKRGSPENDVVRESYVCLTLAGVMVSGFATDLIGIHSIFGAFVFGLTIPKDGEFGQRLIERIEDFVSGLLLPLYFATSGLKTDVAKIRGAESWGMLGLVVVTACAGKIVGTFVVAVMVKVPAREALTLGFLMNTKGLVELIVLNIGKEKKVLNDETFAILVLMALFTTFITTPTVMAIYKPARGTHRKLKDLSASQDSTKEELRILACLHGPANVSSLISLVESIRTTKILRLKLFVMHLMELTERSSSIIMVQRARKNGLPFVHRYRHGERHSNVIGGFEAYRQLGRVAVRPITAVSPLPTMHEDICHMADTKRVTMIILPFHKRWNADHGHSHHHQDGGGDGNVPENVGHGWRLVNQRVLKNAPCSVAVLVDRGLGSIEAQTLSLDGSNVVERVCVIFFGGPDDRESIELGGRMAEHPAVKVTVIRFLVRETLRSTAVTLRPAPSKGKEKNYAFLTTNVDPEKEKELDEGALEDFKSKWKEMVEYKEKEPNNIIEEILSIGQSKDFDLIVVGRGRIPSAEVAALAERQAEHPELGPIGDVLASSINHIIPSILVVQQHNKAHVEDITVSKIVSESSLSINGDTNV

Target ORF information:

RefSeq Version	NM_115232.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cation/H+ exchanger 20 (CHX20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_115232.3

ORF Insert Sequence:

ATGCCCTTCA ACATAACCTC CGTGAAAACC TCATCTAACG GAGTATGGCA AGGCGACAAT 
CCTTTAAACT TCGCTTTTCC GTTACTCATC GTCCAAACGG CGTTAATCAT CGCCGTCAGT 
CGCTTCCTCG CCGTCTTATT CAAACCTCTC CGTCAACCCA AAGTCATCGC CGAGATTGTC 
GGAGGGATTT TGTTAGGACC ATCGGCTTTA GGTAGAAACA TGGCGTACAT GGACCGTATA 
TTTCCGAAAT GGAGTATGCC GATACTCGAA TCCGTCGCGA GCATAGGACT TCTCTTCTTC 
CTCTTCCTCG TCGGTCTAGA ACTCGATTTA TCATCGATCC GACGAAGCGG CAAACGCGCT 
TTCGGAATCG CAGTCGCTGG AATTACACTA CCGTTTATCG CCGGCGTCGG AGTCGCGTTT 
GTGATCCGTA ACACTCTCTA CACCGCCGCG GATAAACCAG GTTACGCCGA GTTTCTCGTT 
TTCATGGGAG TCGCACTCTC GATCACAGCT TTTCCGGTAC TTGCGCGTAT TTTAGCAGAG 
CTCAAGCTTT TAACGACTCA GATAGGAGAA ACCGCGATGG CTGCAGCCGC TTTTAACGAT 
GTAGCCGCGT GGATTTTACT CGCTTTAGCG GTTGCGTTAG CGGGTAATGG CGGTGAGGGA 
GGTGGAGAGA AAAAGAGTCC GTTAGTGTCG TTGTGGGTTT TGTTATCGGG CGCTGGGTTT 
GTGGTTTTTA TGTTGGTTGT GATCCGACCC GGAATGAAAT GGGTCGCGAA ACGTGGATCT 
CCTGAAAACG ACGTCGTACG CGAGTCTTAC GTGTGTTTGA CGTTAGCCGG TGTTATGGTT 
TCCGGTTTCG CGACGGATTT AATTGGGATT CATTCGATTT TTGGAGCGTT TGTTTTCGGT 
TTGACTATAC CGAAAGATGG AGAGTTTGGT CAGCGATTGA TTGAACGAAT TGAGGATTTT 
GTTTCCGGTT TACTCTTACC GCTTTATTTC GCTACGAGTG GTTTGAAGAC TGACGTGGCT 
AAGATTAGAG GAGCTGAGTC GTGGGGGATG TTGGGTCTTG TTGTTGTTAC GGCTTGTGCC 
GGGAAGATAG TCGGAACTTT TGTTGTGGCG GTGATGGTTA AAGTTCCGGC GAGAGAGGCG 
TTGACACTTG GTTTCTTGAT GAATACTAAA GGTTTAGTGG AGCTCATTGT ACTCAACATA 
GGCAAGGAGA AAAAGGTACT AAACGACGAG ACGTTTGCAA TACTAGTGCT AATGGCACTC 
TTCACAACGT TCATAACGAC GCCTACTGTA ATGGCCATTT ACAAGCCGGC ACGTGGCACC 
CACCGCAAAC TAAAAGACTT GTCGGCGAGC CAAGACTCCA CCAAGGAAGA GCTTCGCATC 
CTAGCCTGCC TCCACGGCCC AGCCAATGTC TCCTCCCTCA TCTCTCTCGT CGAGTCCATC 
CGAACCACCA AGATACTACG GCTAAAGCTG TTTGTGATGC ATCTGATGGA ACTAACGGAA 
CGGTCTTCGT CAATCATAAT GGTGCAAAGA GCCCGTAAAA ACGGACTTCC TTTCGTTCAC 
CGTTACCGTC ATGGTGAGCG TCACAGCAAC GTCATAGGAG GCTTCGAAGC CTATCGTCAA 
CTAGGCCGGG TCGCAGTCCG GCCCATCACC GCAGTCTCTC CATTACCCAC AATGCACGAA 
GACATTTGCC ACATGGCAGA TACCAAGAGG GTCACAATGA TCATTTTACC TTTCCACAAA 
CGATGGAACG CTGATCATGG TCATAGCCAC CACCACCAAG ACGGAGGAGG AGATGGAAAC 
GTACCGGAAA ACGTTGGTCA TGGTTGGCGA TTGGTTAACC AAAGGGTTTT GAAGAATGCG 
CCGTGTTCGG TGGCGGTTCT TGTAGACCGT GGACTTGGGT CCATTGAGGC CCAAACTTTG 
AGCTTAGATG GGTCGAATGT GGTTGAAAGG GTTTGTGTGA TTTTCTTTGG TGGGCCTGAT 
GACCGTGAGT CTATAGAGCT CGGCGGGAGA ATGGCTGAGC ATCCGGCCGT TAAAGTTACC 
GTTATTAGGT TTTTGGTAAG AGAAACGTTG AGGAGTACCG CCGTCACTTT ACGACCGGCA 
CCGTCTAAAG GCAAGGAGAA GAACTATGCC TTTTTAACAA CCAACGTGGA TCCAGAAAAA 
GAAAAGGAAT TAGACGAAGG GGCATTGGAA GACTTCAAGA GCAAATGGAA AGAAATGGTG 
GAGTACAAAG AAAAGGAACC AAACAACATC ATTGAAGAAA TACTGTCAAT AGGACAGAGT 
AAAGACTTTG ATCTAATAGT GGTTGGAAGA GGGAGGATAC CGTCGGCCGA GGTGGCGGCA 
TTAGCTGAGC GTCAAGCTGA ACATCCTGAG TTAGGTCCTA TCGGAGACGT GCTCGCCTCT 
TCGATCAACC ACATCATTCC ATCAATCCTT GTGGTTCAAC AACACAACAA AGCTCATGTA 
GAGGATATTA CGGTTTCCAA AATTGTTAGT GAGTCTTCTC TAAGTATTAA CGGAGACACA 
AATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHX20 ( NM_001339628.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001325440.1 Arabidopsis thaliana cation/H+ exchanger 20 (CHX20), mRNA.

Price & Availability↑

CloneID	OAb38314
Clone ID Related Accession (Same CDS sequence)	NM_001339628.1
Accession Version	NM_001339628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	cation/H+ exchanger 20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074).

ATGGCGTACA TGGACCGTAT ATTTCCGAAA TGGAGTATGC CGATACTCGA ATCCGTCGCG 
AGCATAGGAC TTCTCTTCTT CCTCTTCCTC GTCGGTCTAG AACTCGATTT ATCATCGATC 
CGACGAAGCG GCAAACGCGC TTTCGGAATC GCAGTCGCTG GAATTACACT ACCGTTTATC 
GCCGGCGTCG GAGTCGCGTT TGTGATCCGT AACACTCTCT ACACCGCCGC GGATAAACCA 
GGTTACGCCG AGTTTCTCGT TTTCATGGGA GTCGCACTCT CGATCACAGC TTTTCCGGTA 
CTTGCGCGTA TTTTAGCAGA GCTCAAGCTT TTAACGACTC AGATAGGAGA AACCGCGATG 
GCTGCAGCCG CTTTTAACGA TGTAGCCGCG TGGATTTTAC TCGCTTTAGC GGTTGCGTTA 
GCGGGTAATG GCGGTGAGGG AGGTGGAGAG AAAAAGAGTC CGTTAGTGTC GTTGTGGGTT 
TTGTTATCGG GCGCTGGGTT TGTGGTTTTT ATGTTGGTTG TGATCCGACC CGGAATGAAA 
TGGGTCGCGA AACGTGGATC TCCTGAAAAC GACGTCGTAC GCGAGTCTTA CGTGTGTTTG 
ACGTTAGCCG GTGTTATGGT TTCCGGTTTC GCGACGGATT TAATTGGGAT TCATTCGATT 
TTTGGAGCGT TTGTTTTCGG TTTGACTATA CCGAAAGATG GAGAGTTTGG TCAGCGATTG 
ATTGAACGAA TTGAGGATTT TGTTTCCGGT TTACTCTTAC CGCTTTATTT CGCTACGAGT 
GGTTTGAAGA CTGACGTGGC TAAGATTAGA GGAGCTGAGT CGTGGGGGAT GTTGGGTCTT 
GTTGTTGTTA CGGCTTGTGC CGGGAAGATA GTCGGAACTT TTGTTGTGGC GGTGATGGTT 
AAAGTTCCGG CGAGAGAGGC GTTGACACTT GGTTTCTTGA TGAATACTAA AGGTTTAGTG 
GAGCTCATTG TACTCAACAT AGGCAAGGAG AAAAAGGTAC TAAACGACGA GACGTTTGCA 
ATACTAGTGC TAATGGCACT CTTCACAACG TTCATAACGA CGCCTACTGT AATGGCCATT 
TACAAGCCGG CACGTGGCAC CCACCGCAAA CTAAAAGACT TGTCGGCGAG CCAAGACTCC 
ACCAAGGAAG AGCTTCGCAT CCTAGCCTGC CTCCACGGCC CAGCCAATGT CTCCTCCCTC 
ATCTCTCTCG TCGAGTCCAT CCGAACCACC AAGATACTAC GGCTAAAGCT GTTTGTGATG 
CATCTGATGG AACTAACGGA ACGGTCTTCG TCAATCATAA TGGTGCAAAG AGCCCGTAAA 
AACGGACTTC CTTTCGTTCA CCGTTACCGT CATGGTGAGC GTCACAGCAA CGTCATAGGA 
GGCTTCGAAG CCTATCGTCA ACTAGGCCGG GTCGCAGTCC GGCCCATCAC CGCAGTCTCT 
CCATTACCCA CAATGCACGA AGACATTTGC CACATGGCAG ATACCAAGAG GGTCACAATG 
ATCATTTTAC CTTTCCACAA ACGATGGAAC GCTGATCATG GTCATAGCCA CCACCACCAA 
GACGGAGGAG GAGATGGAAA CGTACCGGAA AACGTTGGTC ATGGTTGGCG ATTGGTTAAC 
CAAAGGGTTT TGAAGAATGC GCCGTGTTCG GTGGCGGTTC TTGTAGACCG TGGACTTGGG 
TCCATTGAGG CCCAAACTTT GAGCTTAGAT GGGTCGAATG TGGTTGAAAG GGTTTGTGTG 
ATTTTCTTTG GTGGGCCTGA TGACCGTGAG TCTATAGAGC TCGGCGGGAG AATGGCTGAG 
CATCCGGCCG TTAAAGTTAC CGTTATTAGG TTTTTGGTAA GAGAAACGTT GAGGAGTACC 
GCCGTCACTT TACGACCGGC ACCGTCTAAA GGCAAGGAGA AGAACTATGC CTTTTTAACA 
ACCAACGTGG ATCCAGAAAA AGAAAAGGAA TTAGACGAAG GGGCATTGGA AGACTTCAAG 
AGCAAATGGA AAGAAATGGT GGAGTACAAA GAAAAGGAAC CAAACAACAT CATTGAAGAA 
ATACTGTCAA TAGGACAGAG TAAAGACTTT GATCTAATAG TGGTTGGAAG AGGGAGGATA 
CCGTCGGCCG AGGTGGCGGC ATTAGCTGAG CGTCAAGCTG AACATCCTGA GTTAGGTCCT 
ATCGGAGACG TGCTCGCCTC TTCGATCAAC CACATCATTC CATCAATCCT TGTGGTTCAA 
CAACACAACA AAGCTCATGT AGAGGATATT ACGGTTTCCA AAATTGTTAG TGAGTCTTCT 
CTAAGTATTA ACGGAGACAC AAATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001325440.1
CDS	539..2848
Translation	MAYMDRIFPKWSMPILESVASIGLLFFLFLVGLELDLSSIRRSGKRAFGIAVAGITLPFIAGVGVAFVIRNTLYTAADKPGYAEFLVFMGVALSITAFPVLARILAELKLLTTQIGETAMAAAAFNDVAAWILLALAVALAGNGGEGGGEKKSPLVSLWVLLSGAGFVVFMLVVIRPGMKWVAKRGSPENDVVRESYVCLTLAGVMVSGFATDLIGIHSIFGAFVFGLTIPKDGEFGQRLIERIEDFVSGLLLPLYFATSGLKTDVAKIRGAESWGMLGLVVVTACAGKIVGTFVVAVMVKVPAREALTLGFLMNTKGLVELIVLNIGKEKKVLNDETFAILVLMALFTTFITTPTVMAIYKPARGTHRKLKDLSASQDSTKEELRILACLHGPANVSSLISLVESIRTTKILRLKLFVMHLMELTERSSSIIMVQRARKNGLPFVHRYRHGERHSNVIGGFEAYRQLGRVAVRPITAVSPLPTMHEDICHMADTKRVTMIILPFHKRWNADHGHSHHHQDGGGDGNVPENVGHGWRLVNQRVLKNAPCSVAVLVDRGLGSIEAQTLSLDGSNVVERVCVIFFGGPDDRESIELGGRMAEHPAVKVTVIRFLVRETLRSTAVTLRPAPSKGKEKNYAFLTTNVDPEKEKELDEGALEDFKSKWKEMVEYKEKEPNNIIEEILSIGQSKDFDLIVVGRGRIPSAEVAALAERQAEHPELGPIGDVLASSINHIIPSILVVQQHNKAHVEDITVSKIVSESSLSINGDTNV

Target ORF information:

RefSeq Version	NM_001339628.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana cation/H+ exchanger 20 (CHX20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001339628.1

ORF Insert Sequence:

ATGGCGTACA TGGACCGTAT ATTTCCGAAA TGGAGTATGC CGATACTCGA ATCCGTCGCG 
AGCATAGGAC TTCTCTTCTT CCTCTTCCTC GTCGGTCTAG AACTCGATTT ATCATCGATC 
CGACGAAGCG GCAAACGCGC TTTCGGAATC GCAGTCGCTG GAATTACACT ACCGTTTATC 
GCCGGCGTCG GAGTCGCGTT TGTGATCCGT AACACTCTCT ACACCGCCGC GGATAAACCA 
GGTTACGCCG AGTTTCTCGT TTTCATGGGA GTCGCACTCT CGATCACAGC TTTTCCGGTA 
CTTGCGCGTA TTTTAGCAGA GCTCAAGCTT TTAACGACTC AGATAGGAGA AACCGCGATG 
GCTGCAGCCG CTTTTAACGA TGTAGCCGCG TGGATTTTAC TCGCTTTAGC GGTTGCGTTA 
GCGGGTAATG GCGGTGAGGG AGGTGGAGAG AAAAAGAGTC CGTTAGTGTC GTTGTGGGTT 
TTGTTATCGG GCGCTGGGTT TGTGGTTTTT ATGTTGGTTG TGATCCGACC CGGAATGAAA 
TGGGTCGCGA AACGTGGATC TCCTGAAAAC GACGTCGTAC GCGAGTCTTA CGTGTGTTTG 
ACGTTAGCCG GTGTTATGGT TTCCGGTTTC GCGACGGATT TAATTGGGAT TCATTCGATT 
TTTGGAGCGT TTGTTTTCGG TTTGACTATA CCGAAAGATG GAGAGTTTGG TCAGCGATTG 
ATTGAACGAA TTGAGGATTT TGTTTCCGGT TTACTCTTAC CGCTTTATTT CGCTACGAGT 
GGTTTGAAGA CTGACGTGGC TAAGATTAGA GGAGCTGAGT CGTGGGGGAT GTTGGGTCTT 
GTTGTTGTTA CGGCTTGTGC CGGGAAGATA GTCGGAACTT TTGTTGTGGC GGTGATGGTT 
AAAGTTCCGG CGAGAGAGGC GTTGACACTT GGTTTCTTGA TGAATACTAA AGGTTTAGTG 
GAGCTCATTG TACTCAACAT AGGCAAGGAG AAAAAGGTAC TAAACGACGA GACGTTTGCA 
ATACTAGTGC TAATGGCACT CTTCACAACG TTCATAACGA CGCCTACTGT AATGGCCATT 
TACAAGCCGG CACGTGGCAC CCACCGCAAA CTAAAAGACT TGTCGGCGAG CCAAGACTCC 
ACCAAGGAAG AGCTTCGCAT CCTAGCCTGC CTCCACGGCC CAGCCAATGT CTCCTCCCTC 
ATCTCTCTCG TCGAGTCCAT CCGAACCACC AAGATACTAC GGCTAAAGCT GTTTGTGATG 
CATCTGATGG AACTAACGGA ACGGTCTTCG TCAATCATAA TGGTGCAAAG AGCCCGTAAA 
AACGGACTTC CTTTCGTTCA CCGTTACCGT CATGGTGAGC GTCACAGCAA CGTCATAGGA 
GGCTTCGAAG CCTATCGTCA ACTAGGCCGG GTCGCAGTCC GGCCCATCAC CGCAGTCTCT 
CCATTACCCA CAATGCACGA AGACATTTGC CACATGGCAG ATACCAAGAG GGTCACAATG 
ATCATTTTAC CTTTCCACAA ACGATGGAAC GCTGATCATG GTCATAGCCA CCACCACCAA 
GACGGAGGAG GAGATGGAAA CGTACCGGAA AACGTTGGTC ATGGTTGGCG ATTGGTTAAC 
CAAAGGGTTT TGAAGAATGC GCCGTGTTCG GTGGCGGTTC TTGTAGACCG TGGACTTGGG 
TCCATTGAGG CCCAAACTTT GAGCTTAGAT GGGTCGAATG TGGTTGAAAG GGTTTGTGTG 
ATTTTCTTTG GTGGGCCTGA TGACCGTGAG TCTATAGAGC TCGGCGGGAG AATGGCTGAG 
CATCCGGCCG TTAAAGTTAC CGTTATTAGG TTTTTGGTAA GAGAAACGTT GAGGAGTACC 
GCCGTCACTT TACGACCGGC ACCGTCTAAA GGCAAGGAGA AGAACTATGC CTTTTTAACA 
ACCAACGTGG ATCCAGAAAA AGAAAAGGAA TTAGACGAAG GGGCATTGGA AGACTTCAAG 
AGCAAATGGA AAGAAATGGT GGAGTACAAA GAAAAGGAAC CAAACAACAT CATTGAAGAA 
ATACTGTCAA TAGGACAGAG TAAAGACTTT GATCTAATAG TGGTTGGAAG AGGGAGGATA 
CCGTCGGCCG AGGTGGCGGC ATTAGCTGAG CGTCAAGCTG AACATCCTGA GTTAGGTCCT 
ATCGGAGACG TGCTCGCCTC TTCGATCAAC CACATCATTC CATCAATCCT TGTGGTTCAA 
CAACACAACA AAGCTCATGT AGAGGATATT ACGGTTTCCA AAATTGTTAG TGAGTCTTCT 
CTAAGTATTA ACGGAGACAC AAATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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CHX20 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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