PHS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

The following PHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAb01272 NM_114607.3
Latest version!
Arabidopsis thaliana cytidine/deoxycytidylate deaminase family protein (PHS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OAb05623 NM_001203097.2
Latest version!
Arabidopsis thaliana cytidine/deoxycytidylate deaminase family protein (PHS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAb01272
    Clone ID Related Accession (Same CDS sequence) NM_114607.3
    Accession Version NM_114607.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product cytidine/deoxycytidylate deaminase family protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_114607.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTCTTT CATTTCGAAT TTCTTCGTCG TCTCCGTTAA TCTGCAGAGC CACTCTCTCA 
    AACGGCGACA ACAGTCGTAA CTACCACACG ACTGATGCCG CTTTTATCCG ACGCGCCGCC
    GATTTGTCCG AAATGTCCGC CGGACTTACT TCACCTCACC CTAATTTCGG CTGTGTAATC
    GCCACTTCTT CCGGTAAAGT CGCCGGAGAA GGATATCTAT ACGCTCAAGG TACTAAACCG
    GCGGAAGCGT TGGCTGTTGA AGCTGCCGGA GAGTTTTCTA GAGGCGCCAC TGCTTATTTA
    AACATGGAGC CTGGTGATTG TCATGGTGAC CACACTGCTG TATCTGCTCT TGTTCAGGCA
    GGAATTGAAC GAGTTGTTGT TGGAATAAGG CATCCTTTGC AACATCTTAG AGGCTCTGCG
    ATTCGTGAAT TAAGAAGTCA TGGTATCGAA GTTAATGTTC TTGGAGAAGA CTTTGAGAGT
    AAAGTTCTTG AGGATGCTCG GAAATCGTGT CTTCTTGTGA ATGCTCCTTT GATTCATAGA
    GCTTGTTCTC GAGTTCCGTT CTCTGTTCTT AAGTATGCCA TGACTCTTGA TGGTAAGATT
    GCAGCGAGTA GTGGACATGC AGCATGGATA AGTAGTAAAC TCTCTAGAAC CCGAGTGTTC
    GAGTTGCGAG GAGGAAGTGA TGCTGTGATA GTTGGAGGAA ACACAGTACG CCAAGATGAT
    CCTCGATTGA CTGCAAGACA CGGACAGGGT CACACCCCTA CACGAATTGT GATGACTCAG
    AGTCTTGATC TTCCTGAGAA AGCTAATCTA TGGGATGTCT CGGAAGTATC TACCATAGTT
    GTTACCCAAA GGGGTGCAAG AAAGAGTTTC CAGAAATTGC TTGCGTCCAA GGGTGTTGAA
    GTTGTGGAGT TTGACATGTT GAATCCAAGA GAAGTTATGG AATATTTCCA TCTCCGTGGA
    TATCTTTCTA TACTATGGGA GTGTGGGGGA ACATTAGCTG CATCTGCTAT ATCATCCAGT
    GTCATCCACA AGGTTGTTGC TTTCGTTGCT CCAAAAATTA TCGGTGGGAG TAAAGCACCT
    TCCCCTGTGG GTGACCTTGG GATGGTAGAG ATGACACAAG CATTGAATTT AATTGATGTT
    TGTTATGAGC AGGTTGGTCC CGACATGCTT GTCAGCGGAT TTCTTCAGCC CATACCAGAC
    CTCTTACCTG TTATCCCATC AGAGGATGCA ACTGTCGAGA TTGACCCCAG TGTTGACCCC
    TTTGAACCTA GTATCATATT CTTCTACAAA ACATGGGACC TCTATGGAAT GTGGAACATT
    ACTATCAGGT ACCACACAAC AGTCCATGTA AAATGGTATC TTGCTCTTTC AAAAAAACAT
    AATCTCTTGA TCCTTCACCC AAAAACTTTA AAGGCAAACA AGTTTGTTGG GGTCGAAAAT
    CCAAAGGCAT ATGATTGCGT TGAGAAAATT AGAACAGCTA GAAGTCCAGA GGAGGCAGCT
    CTGATAGGCA GATCAACACT GAGACAGAAA CCTGAACTGG TTCGGAATGA TTGGGAAGAT
    GTGAAGATAG AAGTAATGTA CAAGGCTTTA AAATGCAAGT TCTCAACTTA TCCTCACTTG
    AAATCAATGT TGCTCTCGAC AATTGGAACA GTTCTCGTGG AGGCTTCACC GCACGATCTA
    TTCTGGGGAG GTGGCCGTGA AGGAGAAGGG CTTAACTATC TTGGTCGACT GCTTATGCAG
    CTTCGTTCTG AGTATCTGGG CGAATCCTCT GTTTCAGCTG AAAAGACTTC TTCGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_190323.1
    CDS383..2182
    Translation

    Target ORF information:

    RefSeq Version NM_114607.3
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana cytidine/deoxycytidylate deaminase family protein (PHS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_114607.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTCTTT CATTTCGAAT TTCTTCGTCG TCTCCGTTAA TCTGCAGAGC CACTCTCTCA 
    AACGGCGACA ACAGTCGTAA CTACCACACG ACTGATGCCG CTTTTATCCG ACGCGCCGCC
    GATTTGTCCG AAATGTCCGC CGGACTTACT TCACCTCACC CTAATTTCGG CTGTGTAATC
    GCCACTTCTT CCGGTAAAGT CGCCGGAGAA GGATATCTAT ACGCTCAAGG TACTAAACCG
    GCGGAAGCGT TGGCTGTTGA AGCTGCCGGA GAGTTTTCTA GAGGCGCCAC TGCTTATTTA
    AACATGGAGC CTGGTGATTG TCATGGTGAC CACACTGCTG TATCTGCTCT TGTTCAGGCA
    GGAATTGAAC GAGTTGTTGT TGGAATAAGG CATCCTTTGC AACATCTTAG AGGCTCTGCG
    ATTCGTGAAT TAAGAAGTCA TGGTATCGAA GTTAATGTTC TTGGAGAAGA CTTTGAGAGT
    AAAGTTCTTG AGGATGCTCG GAAATCGTGT CTTCTTGTGA ATGCTCCTTT GATTCATAGA
    GCTTGTTCTC GAGTTCCGTT CTCTGTTCTT AAGTATGCCA TGACTCTTGA TGGTAAGATT
    GCAGCGAGTA GTGGACATGC AGCATGGATA AGTAGTAAAC TCTCTAGAAC CCGAGTGTTC
    GAGTTGCGAG GAGGAAGTGA TGCTGTGATA GTTGGAGGAA ACACAGTACG CCAAGATGAT
    CCTCGATTGA CTGCAAGACA CGGACAGGGT CACACCCCTA CACGAATTGT GATGACTCAG
    AGTCTTGATC TTCCTGAGAA AGCTAATCTA TGGGATGTCT CGGAAGTATC TACCATAGTT
    GTTACCCAAA GGGGTGCAAG AAAGAGTTTC CAGAAATTGC TTGCGTCCAA GGGTGTTGAA
    GTTGTGGAGT TTGACATGTT GAATCCAAGA GAAGTTATGG AATATTTCCA TCTCCGTGGA
    TATCTTTCTA TACTATGGGA GTGTGGGGGA ACATTAGCTG CATCTGCTAT ATCATCCAGT
    GTCATCCACA AGGTTGTTGC TTTCGTTGCT CCAAAAATTA TCGGTGGGAG TAAAGCACCT
    TCCCCTGTGG GTGACCTTGG GATGGTAGAG ATGACACAAG CATTGAATTT AATTGATGTT
    TGTTATGAGC AGGTTGGTCC CGACATGCTT GTCAGCGGAT TTCTTCAGCC CATACCAGAC
    CTCTTACCTG TTATCCCATC AGAGGATGCA ACTGTCGAGA TTGACCCCAG TGTTGACCCC
    TTTGAACCTA GTATCATATT CTTCTACAAA ACATGGGACC TCTATGGAAT GTGGAACATT
    ACTATCAGGT ACCACACAAC AGTCCATGTA AAATGGTATC TTGCTCTTTC AAAAAAACAT
    AATCTCTTGA TCCTTCACCC AAAAACTTTA AAGGCAAACA AGTTTGTTGG GGTCGAAAAT
    CCAAAGGCAT ATGATTGCGT TGAGAAAATT AGAACAGCTA GAAGTCCAGA GGAGGCAGCT
    CTGATAGGCA GATCAACACT GAGACAGAAA CCTGAACTGG TTCGGAATGA TTGGGAAGAT
    GTGAAGATAG AAGTAATGTA CAAGGCTTTA AAATGCAAGT TCTCAACTTA TCCTCACTTG
    AAATCAATGT TGCTCTCGAC AATTGGAACA GTTCTCGTGG AGGCTTCACC GCACGATCTA
    TTCTGGGGAG GTGGCCGTGA AGGAGAAGGG CTTAACTATC TTGGTCGACT GCTTATGCAG
    CTTCGTTCTG AGTATCTGGG CGAATCCTCT GTTTCAGCTG AAAAGACTTC TTCGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OAb05623
    Clone ID Related Accession (Same CDS sequence) NM_001203097.2
    Accession Version NM_001203097.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-13
    Organism Arabidopsis thaliana(thale cress)
    Product cytidine/deoxycytidylate deaminase family protein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_001203097.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTCTTT CATTTCGAAT TTCTTCGTCG TCTCCGTTAA TCTGCAGAGC CACTCTCTCA 
    AACGGCGACA ACAGTCGTAA CTACCACACG ACTGATGCCG CTTTTATCCG ACGCGCCGCC
    GATTTGTCCG AAATGTCCGC CGGACTTACT TCACCTCACC CTAATTTCGG CTGTGTAATC
    GCCACTTCTT CCGGTAAAGT CGCCGGAGAA GGATATCTAT ACGCTCAAGG TACTAAACCG
    GCGGAAGCGT TGGCTGTTGA AGCTGCCGGA GAGTTTTCTA GAGGCGCCAC TGCTTATTTA
    AACATGGAGC CTGGTGATTG TCATGGTGAC CACACTGCTG TATCTGCTCT TGTTCAGGCA
    GGAATTGAAC GAGTTGTTGT TGGAATAAGG CATCCTTTGC AACATCTTAG AGGCTCTGCG
    ATTCGTGAAT TAAGAAGTCA TGGTATCGAA GTTAATGTTC TTGGAGAAGA CTTTGAGAGT
    AAAGTTCTTG AGGATGCTCG GAAATCGTGT CTTCTTGTGA ATGCTCCTTT GATTCATAGA
    GCTTGTTCTC GAGTTCCGTT CTCTGTTCTT AAGTATGCCA TGACTCTTGA TGGTAAGATT
    GCAGCGAGTA GTGGACATGC AGCATGGATA AGTAGTAAAC TCTCTAGAAC CCGAGTGTTC
    GAGTTGCGAG GAGGAAGTGA TGCTGTGATA GTTGGAGGAA ACACAGTACG CCAAGATGAT
    CCTCGATTGA CTGCAAGACA CGGACAGGGT CACACCCCTA CACGAATTGT GATGACTCAG
    AGTCTTGATC TTCCTGAGAA AGCTAATCTA TGGGATGTCT CGGAAGTATC TACCATAGTT
    GTTACCCAAA GGGGTGCAAG AAAGAGTTTC CAGAAATTGC TTGCGTCCAA GGGTGTTGAA
    GTTGTGGAGT TTGACATGTT GAATCCAAGA GAAGTTATGG AATATTTCCA TCTCCGTGGA
    TATCTTTCTA TACTATGGGA GTGTGGGGGA ACATTAGCTG CATCTGCTAT ATCATCCAGT
    GTCATCCACA AGGTTGTTGC TTTCGTTGCT CCAAAAATTA TCGGTGGGAG TAAAGCACCT
    TCCCCTGTGG GTGACCTTGG GATGGTAGAG ATGACACAAG CATTGAATTT AATTGATGTT
    TGTTATGAGC AGGTTGGTCC CGACATGCTT GTCAGCGGAT TTCTTCAGCC CATACCAGAC
    CTCTTACCTG TTATCCCATC AGAGGATGCA ACTGTCGAGA TTGACCCCAG TGTTGACCCC
    TTTGAACCTA GTATCATATT CTTCTACAAA ACATGGGACC TCTATGGAAG TTTCTCAAAT
    TTTTCTCCAC ATCCAATTCG AATGCCCTAT GGCGAGGAGA ATGATGATTA CATGACCTGG
    TCCAGTGTGG AACATTACTA TCAGGCAAAC AAGTTTGTTG GGGTCGAAAA TCCAAAGGCA
    TATGATTGCG TTGAGAAAAT TAGAACAGCT AGAAGTCCAG AGGAGGCAGC TCTGATAGGC
    AGATCAACAC TGAGACAGAA ACCTGAACTG GTTCGGAATG ATTGGGAAGA TGTGAAGATA
    GAAGTAATGT ACAAGGCTTT AAAATGCAAG TTCTCAACTT ATCCTCACTT GAAATCAATG
    TTGCTCTCGA CAATTGGAAC AGTTCTCGTG GAGGCTTCAC CGCACGATCT ATTCTGGGGA
    GGTGGCCGTG AAGGAGAAGG GCTTAACTAT CTTGGTCGAC TGCTTATGCA GCTTCGTTCT
    GAGTATCTGG GCGAATCCTC TGTTTCAGCT GAAAAGACTT CTTCGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001190026.1
    CDS383..2173
    Translation

    Target ORF information:

    RefSeq Version NM_001203097.2
    Organism Arabidopsis thaliana(thale cress)
    Definition Arabidopsis thaliana cytidine/deoxycytidylate deaminase family protein (PHS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001203097.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTCTTT CATTTCGAAT TTCTTCGTCG TCTCCGTTAA TCTGCAGAGC CACTCTCTCA 
    AACGGCGACA ACAGTCGTAA CTACCACACG ACTGATGCCG CTTTTATCCG ACGCGCCGCC
    GATTTGTCCG AAATGTCCGC CGGACTTACT TCACCTCACC CTAATTTCGG CTGTGTAATC
    GCCACTTCTT CCGGTAAAGT CGCCGGAGAA GGATATCTAT ACGCTCAAGG TACTAAACCG
    GCGGAAGCGT TGGCTGTTGA AGCTGCCGGA GAGTTTTCTA GAGGCGCCAC TGCTTATTTA
    AACATGGAGC CTGGTGATTG TCATGGTGAC CACACTGCTG TATCTGCTCT TGTTCAGGCA
    GGAATTGAAC GAGTTGTTGT TGGAATAAGG CATCCTTTGC AACATCTTAG AGGCTCTGCG
    ATTCGTGAAT TAAGAAGTCA TGGTATCGAA GTTAATGTTC TTGGAGAAGA CTTTGAGAGT
    AAAGTTCTTG AGGATGCTCG GAAATCGTGT CTTCTTGTGA ATGCTCCTTT GATTCATAGA
    GCTTGTTCTC GAGTTCCGTT CTCTGTTCTT AAGTATGCCA TGACTCTTGA TGGTAAGATT
    GCAGCGAGTA GTGGACATGC AGCATGGATA AGTAGTAAAC TCTCTAGAAC CCGAGTGTTC
    GAGTTGCGAG GAGGAAGTGA TGCTGTGATA GTTGGAGGAA ACACAGTACG CCAAGATGAT
    CCTCGATTGA CTGCAAGACA CGGACAGGGT CACACCCCTA CACGAATTGT GATGACTCAG
    AGTCTTGATC TTCCTGAGAA AGCTAATCTA TGGGATGTCT CGGAAGTATC TACCATAGTT
    GTTACCCAAA GGGGTGCAAG AAAGAGTTTC CAGAAATTGC TTGCGTCCAA GGGTGTTGAA
    GTTGTGGAGT TTGACATGTT GAATCCAAGA GAAGTTATGG AATATTTCCA TCTCCGTGGA
    TATCTTTCTA TACTATGGGA GTGTGGGGGA ACATTAGCTG CATCTGCTAT ATCATCCAGT
    GTCATCCACA AGGTTGTTGC TTTCGTTGCT CCAAAAATTA TCGGTGGGAG TAAAGCACCT
    TCCCCTGTGG GTGACCTTGG GATGGTAGAG ATGACACAAG CATTGAATTT AATTGATGTT
    TGTTATGAGC AGGTTGGTCC CGACATGCTT GTCAGCGGAT TTCTTCAGCC CATACCAGAC
    CTCTTACCTG TTATCCCATC AGAGGATGCA ACTGTCGAGA TTGACCCCAG TGTTGACCCC
    TTTGAACCTA GTATCATATT CTTCTACAAA ACATGGGACC TCTATGGAAG TTTCTCAAAT
    TTTTCTCCAC ATCCAATTCG AATGCCCTAT GGCGAGGAGA ATGATGATTA CATGACCTGG
    TCCAGTGTGG AACATTACTA TCAGGCAAAC AAGTTTGTTG GGGTCGAAAA TCCAAAGGCA
    TATGATTGCG TTGAGAAAAT TAGAACAGCT AGAAGTCCAG AGGAGGCAGC TCTGATAGGC
    AGATCAACAC TGAGACAGAA ACCTGAACTG GTTCGGAATG ATTGGGAAGA TGTGAAGATA
    GAAGTAATGT ACAAGGCTTT AAAATGCAAG TTCTCAACTT ATCCTCACTT GAAATCAATG
    TTGCTCTCGA CAATTGGAAC AGTTCTCGTG GAGGCTTCAC CGCACGATCT ATTCTGGGGA
    GGTGGCCGTG AAGGAGAAGG GCTTAACTAT CTTGGTCGAC TGCTTATGCA GCTTCGTTCT
    GAGTATCTGG GCGAATCCTC TGTTTCAGCT GAAAAGACTT CTTCGGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
    Nature408(6814)820-2(2000 Dec)
    Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,