NERD cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NERD
Entrez Gene ID	816147
Full Name	GW repeat- and PHD finger-containing protein NERD
Synonyms	F16F14.1,F16F14_1,Needed for RDR2-independent DNA methylation
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001335477.1	NP_001324854.1	GW repeat- and PHD finger-containing protein NERD
NM_127202.5	NP_179241.4	GW repeat- and PHD finger-containing protein NERD

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT2G16485	NP_179241.4
Oryza sativa (rice)	Os01g0884500	NP_001045018.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A central role for NERD in integrating RNA silencing and chromatin signals in transcriptional silencing is supported by data showing that it binds both to histone H3 and AGO2 proteins and contributes to siRNA accumulation at a NERD-targeted locus. [NERD]
Title: NERD, a plant-specific GW protein, defines an additional RNAi-dependent chromatin-based pathway in Arabidopsis.

The following NERD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NERD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb07117	NM_127202.5 Latest version!	Arabidopsis thaliana GW repeat- and PHD finger-containing protein NERD (NERD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$1021.30 $1459.00
OAb35707	NM_001335477.1 Latest version!	Arabidopsis thaliana GW repeat- and PHD finger-containing protein NERD (NERD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NERD ( NM_127202.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_179241.4 Arabidopsis thaliana GW repeat- and PHD finger-containing protein NERD (NERD), mRNA.

Price & Availability↑

CloneID	OAb07117
Clone ID Related Accession (Same CDS sequence)	NM_127202.5
Accession Version	NM_127202.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	GW repeat- and PHD finger-containing protein NERD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_127202.4.

ATGGATTCTG ATTCTGAGAG AGCTTCTCTG GAGTCGATTA AAGATAATTC TGAATGTGTT 
CATGTTTCCA ACGAACCTAA TCTCACGGCA ACATGCGTTG ATTCGTCTGT TGGAGAGGAA 
GGAGTTACGG ATGTAAATTC GTCCGCCGCT GTGTCGGAGC TCGTGCCGCC GGAGCAAGGA 
GAAGGTGCGC TTTTGAATTC CGTCCCTGAG ATCAGTGAAA GGGGGATTCC TGTTGATGTC 
GTTTCCTCGG TAGATGGAGG AGGAGAAGAA AATGCAGCTT TTAATATTCA GGAGATTGAT 
TCTGTTGGCG GTGACGCTGC GGCGGTGGAG GAGGTTCCTC TGAAATCGTC TTCGGTGGTT 
GGGGAAGGAA GAGAAGAGGA AGCTGGGGCT TCAATCGTCA AAGAAGAGGA TTTTGTAGCT 
GAAGCTAATT TGTCAGGAGA TAGGCTTGAG GAAAACAAAG AAGTTTCCAT GGAAGAAGAA 
CCTTCATCAC ATGAGCTCTC GGTTTGTGAG GTAAACGGAG TTGATTCTTT GAATGATGAG 
GAAAATAGAG AGGTAGGTGA ACAGATAGTT TGTGGAAGTA TGGGTGGTGA GGAGATAGAG 
AGTGATTTAG AGTCTAAAAA GGAAAAGGTA GATGTGATAG AGGAGGAAAC CACAGCGCAA 
GCTGCTTCCT TGGTTAATGC AATTGAAATT CCTGATGATA AAGAGGTAGC TTGCGTGGCT 
GGGTTTACGG AAATTTCCTC TCAAGATAAA GGGTTAGACG AATCAGGAAA TGGATTTTTA 
GATGAAGAGC CAGTGAAAGA GTTGCAAATA GGTGAAGGAG CTAAAGATCT GACAGATGGA 
GATGCAAAAG AAGGTGTGGA TGTTACAGAA GATGAAATGG ATATACAGGT ATTGAAGAAA 
TCTAAGGAGG AAGAAAAGGT TGATAGTACT ACTGAATTAG AGATAGAAAC TATGCGCCTA 
GAGGTGCATG ACGTGGCTAC TGAGATGTCC GACAAAACTG TTATATCATC AGCTGTTGTT 
ACTCAGTTTA CTGGGGAAAC AAGTAATGAT AAGGAAACTG TTATGGATGA TGTCAAAGAA 
GATGTTGACA AGGATTCAGA AGCTGGTAAA TCACTGGACA TACATGTACC AGAGGCTACA 
GAAGAAGTAG ACACAGATGT TAATTACGGT GTTGGGATAG AGAAAGAAGG CGATGGGGTG 
GGTGGTGCGG AAGAAGCAGG GCAGACAGTT GATTTGGAGG AAATTCGAGA AGAAAACCAA 
GAACTGTCTA AGGAACTGGC TCAAGTAGAT GAAACCAAGA TTTCTGAAAT GTCGGAAGTA 
ACGGAGACAA TGATCAAAGA TGAGGATCAG GAGAAAGATG ACAATATGAC TGATTTGGCG 
GAGGATGTGG AAAACCATAG AGATTCCTCA GTAGCTGATA TTGAGGAGGG AAGGGAAGAT 
CATGAAGATA TGGGAGTGAC AGAGACACAG AAGGAGACTG TTTTGGGTAA AGTAGACAGA 
ACAAAAATTG CTGAAGTGTC AGAAGAAACA GACACAAGGA TTGAAGATGA GGATCAGGAG 
AAAGATGATG AGATGACTGA TGTTGCGGAA GATGTGAAAA CGCATGGAGA CTCCTCAGTA 
GCTGATATTG AAGAGGGAAG GGAAAGTCAA GAAGAGATGA CAGAGACACA GGAGGACAGT 
GTTATGGCTG ATGAAGAGCC TGAGGAAGTT GAGGAAGAAA ATAAGAGTGC TGGAGGAAAG 
AGGAAACGGG GGAGAAATAC AAAGACAGTC AAAGGGACAG GAAAGAAGAA AGAGGAAGAT 
GTTTGTTTTA TGTGCTTTGA TGGGGGTGAT CTTGTCCTTT GTGACCGCAG GGGATGCACA 
AAAGCGTACC ACCCTTCCTG TGTAGATCGA GATGAGGCTT TCTTCCAGAC AAAGGGTAAA 
TGGAACTGTG GATGGCACCT ATGTAGCAAA TGTGAGAAAA CTGCAACGTA CTTGTGTTAT 
ACATGTATGT TTTCATTATG CAAGGGATGT GCCAAGGATG CTGTCTTCTT TTGCATCAGA 
GGTAATAAGG GTTTGTGTGA GACATGCATG GAGACAGTCA AGTTGATAGA AAGAAAACAG 
CAGGAAAAGG AGCCGGCGCA GTTGGATTTT AATGACAAGA CCAGCTGGGA ATATCTCTTT 
AAGGATTACT GGATTGATTT GAAAACCCAG CTATCTTTAA GTCCAGAGGA ACTTGATCAG 
GCTAAGAGGC CACTGAAAGG ACATGAGACC AATGCTAGCA AACAGGGAAC AGCCAGTGAG 
ACTGACTATG TTACTGATGG AGGATCAGAT TCAGATAGTT CTCCAAAAAA GAGGAAGACC 
AGGAGTCGAT CAAAGTCTGG TTCTGCTGAG AAAATCCTAT CATCAGGCGA TAAGAATTTG 
TCAGATGAAA CTATGGAGTG GGCTTCCAAG GAACTTTTGG ATCTGGTCGT GCACATGAGA 
CGTGGTGACA GATCTTTCTT ACCTATGTTA GAAGTACAGA CTCTCCTACT GGCTTATATA 
AAAAGATACA ATCTTCGTGA TCCCCGTCGT AAAAGCCAGG TTATCTGCGA CTCCAGGCTT 
CAGAATTTGT TCGGCAAATC ACATGTTGGA CACTTTGAGA TGTTAAATCT TCTTGACTCT 
CATTTTCTGA AAAAAGAGCA AAATCAGGCA GATGATATTC AAGGTGACAT AGTTGATACA 
GAAGAACCTA ATCATGTGGA TGTTGATGAA AACTTGGACC ATCCAGTCAA AAGTGGGAAG 
GATAAGAAAC GTAAAACACG CAAAAAAAAT GTCAGGAAAG GACGTCAATC TAATCTTGAT 
GATTTTGCTG CTGTTGATAT GCACAACATT AATTTGATAT ACTTAAGGCG GAGCTTGGTG 
GAAGATCTAC TTGAAGATTC TACAGCATTC GAAGAAAAAG TTGCTAGTGC ATTTGTTAGG 
TTAAGGATAT CTGGAAATCA AAAGCAAGAT TTATATCGAC TGGTTCAAGT TGTTGGCACA 
AGCAAAGCTC CTGAACCTTA TAAAGTTGGC AAAAAAACAA CAGACTATGT ACTTGAGATA 
TTGAACTTGG ACAAAACGGA AGTGATATCC ATTGACATTA TATCAAATCA AGATTTCACT 
GAGGATGAAT GCAAGCGTCT TAAGCAGAGC ATAAAATGTG GATTAATAAA CAGGCTGACA 
GTGGGTGATA TACAAGAAAA GGCCATTGCA CTGCAGGAAG TGAGAGTCAA AAACTTGCTG 
GAAGCAGAGA TCCTACGTTT CAGTCATTTG CGTGATCGTG CTAGTGACAT GGGCCGCAGA 
AAAGAATATC CTTATCTGTT GAAACTTTCA AATTCCTTAA CTATGCTTAC GCTGCGAGAA 
TGTGTTGAGA AACTGCAACT GTTAAAATCT CCAGAGGAAC GGCAGCGCAG GCTAGAAGAA 
ATTCCAGAAA TACATGCTGA TCCCAAAATG GATCCAGATT GTGAATCGGA AGATGAAGAT 
GAAAAAGAAG AAAAGGAAAA AGAGAAACAA CTGAGGCCGC GAAGCAGTAG CTTTAACAGA 
AGAGGAAGAG ATCCAATATC TCCACGAAAA GGAGGATTTA GTTCCAATGA ATCATGGACT 
GGCACAAGTA ACTACTCAAA TACAAGTGCA AATCGTGAGT TAAGCAGAAG TTATTCTGGT 
AGAGGTTCTA CTGGGAGAGG GGATTATCTT GGTAGTTCTG ACGACAAGGT TAGTGATAGT 
ATGTGGACTT CAGCTAGAGA AAGAGAAGTG CAACCATCTT TAGGCTCTGA GAAGCCAAGA 
TCTGTTTCAA TTCCTGAAAC TCCTGCCAGA AGTTCTCGTG CTATTGCACC ACCAGAATTA 
TCTCCTAGGA TTGCCTCTGA AATTTCAATG GCACCTCCTG CTGTTGTTTC GCAGCCTGTT 
CCCAAGTCAA ATGATTCAGA GAAAATATGG CATTACAAGG ACCCATCTGG TAAAGTTCAA 
GGACCTTTTT CGATGGCGCA GCTCCGTAAA TGGAACAACA CTGGGTACTT CCCTGCTAAG 
TTGGAAATCT GGAAAGCAAA TGAGTCACCG TTGGACTCTG TTCTACTGAC TGATGCATTG 
GCTGGACTAT TTCAGAAGCA AACCCAGGCG GTGGATAATA GCTATATGAA AGCTCAGGTA 
GCAGCTTTTT CTGGTCAATC AAGCCAATCT GAACCCAATT TAGGTTTTGC TGCTAGGATT 
GCTCCAACAA CCATTGAGAT TCCTAGGAAT TCCCAAGATA CTTGGAGTCA AGGTGGTAGT 
CTTCCATCCC CTACTCCAAA TCAAATCACA ACTCCAACAG CAAAAAGGCG AAATTTTGAG 
AGCAGATGGT CTCCAACCAA GCCATCTCCA CAGTCAGCTA ATCAGTCGAT GAATTATTCA 
GTAGCACAAT CTGGACAGAG TCAAACTTCT AGGATAGATA TCCCTGTGGT TGTAAATTCT 
GCTGGTGCTC TACAGCCTCA AACTTACCCT ATTCCAACAC CAGATCCGAT CAACGTTTCT 
GTTAATCACT CTGCGACTCT TCATTCTCCA ACACCAGCAG GTGGAAAGCA AAGCTGGGGG 
AGCATGCAGA CAGATCATGG AGGAAGCAAC ACTCCCTCTT CTCAGAATAA CTCTACGTCT 
TATGGCACCC CATCTCCTAG TGTTCTCCCC TCACAGAGTC AACCAGGATT TCCTCCATCT 
GATTCTTGGA AGGTTGCAGT TCCAAGCCAA CCGAATGCTC AGGCTCAGGC CCAGTGGGGT 
ATGAACATGG TGAACAACAA CCAAAACTCA GCACAACCTC AGGCTCCAGC AAACCAGAAC 
AGCAGCTGGG GTCAAGGGAC TGTAAATCCA AACATGGGAT GGGTTGGTCC TGCTCAAACT 
GGAGTGAATG TAAATTGGGG TGGATCTTCT GTTCCCTCAA CTGTGCAAGG AATCACTCAT 
TCTGGCTGGG TCGCGCCTGT CCAAGGACAA ACACAAGCTT ATCCAAATCC AGGATGGGGC 
CCAACAGGCC ACCCTCAATC TCAATCCCAG TCTCAAGTTC AAGCTCAAGC GGGTACTACG 
GGCTCGGGAT GGATGCAACC AGGTCAAGGG ATTCAGTCTG GAAACAGTAA TCAAAATTGG 
GGAACTCAAA ATCAGACAGC GATCCCAAGC GGAGGGTCAG GAGGGAACCA AGCCGGTTAC 
TGGGGAAACC AACAACAGAG CCAGAATGGA GATTCTGGGT ATGGTTGGAA CAGGCAGTCA 
GGTGGGCAGC AGAACAACTT CAAGGGACAA AGAGTATGCA AGTTCTTCCG CGAAAATGGA 
CATTGTCGGA AAGGAGCATC CTGCAATTAC CTCCACAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_179241.4
CDS	124..5445
Translation	MDSDSERASLESIKDNSECVHVSNEPNLTATCVDSSVGEEGVTDVNSSAAVSELVPPEQGEGALLNSVPEISERGIPVDVVSSVDGGGEENAAFNIQEIDSVGGDAAAVEEVPLKSSSVVGEGREEEAGASIVKEEDFVAEANLSGDRLEENKEVSMEEEPSSHELSVCEVNGVDSLNDEENREVGEQIVCGSMGGEEIESDLESKKEKVDVIEEETTAQAASLVNAIEIPDDKEVACVAGFTEISSQDKGLDESGNGFLDEEPVKELQIGEGAKDLTDGDAKEGVDVTEDEMDIQVLKKSKEEEKVDSTTELEIETMRLEVHDVATEMSDKTVISSAVVTQFTGETSNDKETVMDDVKEDVDKDSEAGKSLDIHVPEATEEVDTDVNYGVGIEKEGDGVGGAEEAGQTVDLEEIREENQELSKELAQVDETKISEMSEVTETMIKDEDQEKDDNMTDLAEDVENHRDSSVADIEEGREDHEDMGVTETQKETVLGKVDRTKIAEVSEETDTRIEDEDQEKDDEMTDVAEDVKTHGDSSVADIEEGRESQEEMTETQEDSVMADEEPEEVEEENKSAGGKRKRGRNTKTVKGTGKKKEEDVCFMCFDGGDLVLCDRRGCTKAYHPSCVDRDEAFFQTKGKWNCGWHLCSKCEKTATYLCYTCMFSLCKGCAKDAVFFCIRGNKGLCETCMETVKLIERKQQEKEPAQLDFNDKTSWEYLFKDYWIDLKTQLSLSPEELDQAKRPLKGHETNASKQGTASETDYVTDGGSDSDSSPKKRKTRSRSKSGSAEKILSSGDKNLSDETMEWASKELLDLVVHMRRGDRSFLPMLEVQTLLLAYIKRYNLRDPRRKSQVICDSRLQNLFGKSHVGHFEMLNLLDSHFLKKEQNQADDIQGDIVDTEEPNHVDVDENLDHPVKSGKDKKRKTRKKNVRKGRQSNLDDFAAVDMHNINLIYLRRSLVEDLLEDSTAFEEKVASAFVRLRISGNQKQDLYRLVQVVGTSKAPEPYKVGKKTTDYVLEILNLDKTEVISIDIISNQDFTEDECKRLKQSIKCGLINRLTVGDIQEKAIALQEVRVKNLLEAEILRFSHLRDRASDMGRRKEYPYLLKLSNSLTMLTLRECVEKLQLLKSPEERQRRLEEIPEIHADPKMDPDCESEDEDEKEEKEKEKQLRPRSSSFNRRGRDPISPRKGGFSSNESWTGTSNYSNTSANRELSRSYSGRGSTGRGDYLGSSDDKVSDSMWTSAREREVQPSLGSEKPRSVSIPETPARSSRAIAPPELSPRIASEISMAPPAVVSQPVPKSNDSEKIWHYKDPSGKVQGPFSMAQLRKWNNTGYFPAKLEIWKANESPLDSVLLTDALAGLFQKQTQAVDNSYMKAQVAAFSGQSSQSEPNLGFAARIAPTTIEIPRNSQDTWSQGGSLPSPTPNQITTPTAKRRNFESRWSPTKPSPQSANQSMNYSVAQSGQSQTSRIDIPVVVNSAGALQPQTYPIPTPDPINVSVNHSATLHSPTPAGGKQSWGSMQTDHGGSNTPSSQNNSTSYGTPSPSVLPSQSQPGFPPSDSWKVAVPSQPNAQAQAQWGMNMVNNNQNSAQPQAPANQNSSWGQGTVNPNMGWVGPAQTGVNVNWGGSSVPSTVQGITHSGWVAPVQGQTQAYPNPGWGPTGHPQSQSQSQVQAQAGTTGSGWMQPGQGIQSGNSNQNWGTQNQTAIPSGGSGGNQAGYWGNQQQSQNGDSGYGWNRQSGGQQNNFKGQRVCKFFRENGHCRKGASCNYLHN

Target ORF information:

RefSeq Version	NM_127202.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana GW repeat- and PHD finger-containing protein NERD (NERD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_127202.5

ORF Insert Sequence:

ATGGATTCTG ATTCTGAGAG AGCTTCTCTG GAGTCGATTA AAGATAATTC TGAATGTGTT 
CATGTTTCCA ACGAACCTAA TCTCACGGCA ACATGCGTTG ATTCGTCTGT TGGAGAGGAA 
GGAGTTACGG ATGTAAATTC GTCCGCCGCT GTGTCGGAGC TCGTGCCGCC GGAGCAAGGA 
GAAGGTGCGC TTTTGAATTC CGTCCCTGAG ATCAGTGAAA GGGGGATTCC TGTTGATGTC 
GTTTCCTCGG TAGATGGAGG AGGAGAAGAA AATGCAGCTT TTAATATTCA GGAGATTGAT 
TCTGTTGGCG GTGACGCTGC GGCGGTGGAG GAGGTTCCTC TGAAATCGTC TTCGGTGGTT 
GGGGAAGGAA GAGAAGAGGA AGCTGGGGCT TCAATCGTCA AAGAAGAGGA TTTTGTAGCT 
GAAGCTAATT TGTCAGGAGA TAGGCTTGAG GAAAACAAAG AAGTTTCCAT GGAAGAAGAA 
CCTTCATCAC ATGAGCTCTC GGTTTGTGAG GTAAACGGAG TTGATTCTTT GAATGATGAG 
GAAAATAGAG AGGTAGGTGA ACAGATAGTT TGTGGAAGTA TGGGTGGTGA GGAGATAGAG 
AGTGATTTAG AGTCTAAAAA GGAAAAGGTA GATGTGATAG AGGAGGAAAC CACAGCGCAA 
GCTGCTTCCT TGGTTAATGC AATTGAAATT CCTGATGATA AAGAGGTAGC TTGCGTGGCT 
GGGTTTACGG AAATTTCCTC TCAAGATAAA GGGTTAGACG AATCAGGAAA TGGATTTTTA 
GATGAAGAGC CAGTGAAAGA GTTGCAAATA GGTGAAGGAG CTAAAGATCT GACAGATGGA 
GATGCAAAAG AAGGTGTGGA TGTTACAGAA GATGAAATGG ATATACAGGT ATTGAAGAAA 
TCTAAGGAGG AAGAAAAGGT TGATAGTACT ACTGAATTAG AGATAGAAAC TATGCGCCTA 
GAGGTGCATG ACGTGGCTAC TGAGATGTCC GACAAAACTG TTATATCATC AGCTGTTGTT 
ACTCAGTTTA CTGGGGAAAC AAGTAATGAT AAGGAAACTG TTATGGATGA TGTCAAAGAA 
GATGTTGACA AGGATTCAGA AGCTGGTAAA TCACTGGACA TACATGTACC AGAGGCTACA 
GAAGAAGTAG ACACAGATGT TAATTACGGT GTTGGGATAG AGAAAGAAGG CGATGGGGTG 
GGTGGTGCGG AAGAAGCAGG GCAGACAGTT GATTTGGAGG AAATTCGAGA AGAAAACCAA 
GAACTGTCTA AGGAACTGGC TCAAGTAGAT GAAACCAAGA TTTCTGAAAT GTCGGAAGTA 
ACGGAGACAA TGATCAAAGA TGAGGATCAG GAGAAAGATG ACAATATGAC TGATTTGGCG 
GAGGATGTGG AAAACCATAG AGATTCCTCA GTAGCTGATA TTGAGGAGGG AAGGGAAGAT 
CATGAAGATA TGGGAGTGAC AGAGACACAG AAGGAGACTG TTTTGGGTAA AGTAGACAGA 
ACAAAAATTG CTGAAGTGTC AGAAGAAACA GACACAAGGA TTGAAGATGA GGATCAGGAG 
AAAGATGATG AGATGACTGA TGTTGCGGAA GATGTGAAAA CGCATGGAGA CTCCTCAGTA 
GCTGATATTG AAGAGGGAAG GGAAAGTCAA GAAGAGATGA CAGAGACACA GGAGGACAGT 
GTTATGGCTG ATGAAGAGCC TGAGGAAGTT GAGGAAGAAA ATAAGAGTGC TGGAGGAAAG 
AGGAAACGGG GGAGAAATAC AAAGACAGTC AAAGGGACAG GAAAGAAGAA AGAGGAAGAT 
GTTTGTTTTA TGTGCTTTGA TGGGGGTGAT CTTGTCCTTT GTGACCGCAG GGGATGCACA 
AAAGCGTACC ACCCTTCCTG TGTAGATCGA GATGAGGCTT TCTTCCAGAC AAAGGGTAAA 
TGGAACTGTG GATGGCACCT ATGTAGCAAA TGTGAGAAAA CTGCAACGTA CTTGTGTTAT 
ACATGTATGT TTTCATTATG CAAGGGATGT GCCAAGGATG CTGTCTTCTT TTGCATCAGA 
GGTAATAAGG GTTTGTGTGA GACATGCATG GAGACAGTCA AGTTGATAGA AAGAAAACAG 
CAGGAAAAGG AGCCGGCGCA GTTGGATTTT AATGACAAGA CCAGCTGGGA ATATCTCTTT 
AAGGATTACT GGATTGATTT GAAAACCCAG CTATCTTTAA GTCCAGAGGA ACTTGATCAG 
GCTAAGAGGC CACTGAAAGG ACATGAGACC AATGCTAGCA AACAGGGAAC AGCCAGTGAG 
ACTGACTATG TTACTGATGG AGGATCAGAT TCAGATAGTT CTCCAAAAAA GAGGAAGACC 
AGGAGTCGAT CAAAGTCTGG TTCTGCTGAG AAAATCCTAT CATCAGGCGA TAAGAATTTG 
TCAGATGAAA CTATGGAGTG GGCTTCCAAG GAACTTTTGG ATCTGGTCGT GCACATGAGA 
CGTGGTGACA GATCTTTCTT ACCTATGTTA GAAGTACAGA CTCTCCTACT GGCTTATATA 
AAAAGATACA ATCTTCGTGA TCCCCGTCGT AAAAGCCAGG TTATCTGCGA CTCCAGGCTT 
CAGAATTTGT TCGGCAAATC ACATGTTGGA CACTTTGAGA TGTTAAATCT TCTTGACTCT 
CATTTTCTGA AAAAAGAGCA AAATCAGGCA GATGATATTC AAGGTGACAT AGTTGATACA 
GAAGAACCTA ATCATGTGGA TGTTGATGAA AACTTGGACC ATCCAGTCAA AAGTGGGAAG 
GATAAGAAAC GTAAAACACG CAAAAAAAAT GTCAGGAAAG GACGTCAATC TAATCTTGAT 
GATTTTGCTG CTGTTGATAT GCACAACATT AATTTGATAT ACTTAAGGCG GAGCTTGGTG 
GAAGATCTAC TTGAAGATTC TACAGCATTC GAAGAAAAAG TTGCTAGTGC ATTTGTTAGG 
TTAAGGATAT CTGGAAATCA AAAGCAAGAT TTATATCGAC TGGTTCAAGT TGTTGGCACA 
AGCAAAGCTC CTGAACCTTA TAAAGTTGGC AAAAAAACAA CAGACTATGT ACTTGAGATA 
TTGAACTTGG ACAAAACGGA AGTGATATCC ATTGACATTA TATCAAATCA AGATTTCACT 
GAGGATGAAT GCAAGCGTCT TAAGCAGAGC ATAAAATGTG GATTAATAAA CAGGCTGACA 
GTGGGTGATA TACAAGAAAA GGCCATTGCA CTGCAGGAAG TGAGAGTCAA AAACTTGCTG 
GAAGCAGAGA TCCTACGTTT CAGTCATTTG CGTGATCGTG CTAGTGACAT GGGCCGCAGA 
AAAGAATATC CTTATCTGTT GAAACTTTCA AATTCCTTAA CTATGCTTAC GCTGCGAGAA 
TGTGTTGAGA AACTGCAACT GTTAAAATCT CCAGAGGAAC GGCAGCGCAG GCTAGAAGAA 
ATTCCAGAAA TACATGCTGA TCCCAAAATG GATCCAGATT GTGAATCGGA AGATGAAGAT 
GAAAAAGAAG AAAAGGAAAA AGAGAAACAA CTGAGGCCGC GAAGCAGTAG CTTTAACAGA 
AGAGGAAGAG ATCCAATATC TCCACGAAAA GGAGGATTTA GTTCCAATGA ATCATGGACT 
GGCACAAGTA ACTACTCAAA TACAAGTGCA AATCGTGAGT TAAGCAGAAG TTATTCTGGT 
AGAGGTTCTA CTGGGAGAGG GGATTATCTT GGTAGTTCTG ACGACAAGGT TAGTGATAGT 
ATGTGGACTT CAGCTAGAGA AAGAGAAGTG CAACCATCTT TAGGCTCTGA GAAGCCAAGA 
TCTGTTTCAA TTCCTGAAAC TCCTGCCAGA AGTTCTCGTG CTATTGCACC ACCAGAATTA 
TCTCCTAGGA TTGCCTCTGA AATTTCAATG GCACCTCCTG CTGTTGTTTC GCAGCCTGTT 
CCCAAGTCAA ATGATTCAGA GAAAATATGG CATTACAAGG ACCCATCTGG TAAAGTTCAA 
GGACCTTTTT CGATGGCGCA GCTCCGTAAA TGGAACAACA CTGGGTACTT CCCTGCTAAG 
TTGGAAATCT GGAAAGCAAA TGAGTCACCG TTGGACTCTG TTCTACTGAC TGATGCATTG 
GCTGGACTAT TTCAGAAGCA AACCCAGGCG GTGGATAATA GCTATATGAA AGCTCAGGTA 
GCAGCTTTTT CTGGTCAATC AAGCCAATCT GAACCCAATT TAGGTTTTGC TGCTAGGATT 
GCTCCAACAA CCATTGAGAT TCCTAGGAAT TCCCAAGATA CTTGGAGTCA AGGTGGTAGT 
CTTCCATCCC CTACTCCAAA TCAAATCACA ACTCCAACAG CAAAAAGGCG AAATTTTGAG 
AGCAGATGGT CTCCAACCAA GCCATCTCCA CAGTCAGCTA ATCAGTCGAT GAATTATTCA 
GTAGCACAAT CTGGACAGAG TCAAACTTCT AGGATAGATA TCCCTGTGGT TGTAAATTCT 
GCTGGTGCTC TACAGCCTCA AACTTACCCT ATTCCAACAC CAGATCCGAT CAACGTTTCT 
GTTAATCACT CTGCGACTCT TCATTCTCCA ACACCAGCAG GTGGAAAGCA AAGCTGGGGG 
AGCATGCAGA CAGATCATGG AGGAAGCAAC ACTCCCTCTT CTCAGAATAA CTCTACGTCT 
TATGGCACCC CATCTCCTAG TGTTCTCCCC TCACAGAGTC AACCAGGATT TCCTCCATCT 
GATTCTTGGA AGGTTGCAGT TCCAAGCCAA CCGAATGCTC AGGCTCAGGC CCAGTGGGGT 
ATGAACATGG TGAACAACAA CCAAAACTCA GCACAACCTC AGGCTCCAGC AAACCAGAAC 
AGCAGCTGGG GTCAAGGGAC TGTAAATCCA AACATGGGAT GGGTTGGTCC TGCTCAAACT 
GGAGTGAATG TAAATTGGGG TGGATCTTCT GTTCCCTCAA CTGTGCAAGG AATCACTCAT 
TCTGGCTGGG TCGCGCCTGT CCAAGGACAA ACACAAGCTT ATCCAAATCC AGGATGGGGC 
CCAACAGGCC ACCCTCAATC TCAATCCCAG TCTCAAGTTC AAGCTCAAGC GGGTACTACG 
GGCTCGGGAT GGATGCAACC AGGTCAAGGG ATTCAGTCTG GAAACAGTAA TCAAAATTGG 
GGAACTCAAA ATCAGACAGC GATCCCAAGC GGAGGGTCAG GAGGGAACCA AGCCGGTTAC 
TGGGGAAACC AACAACAGAG CCAGAATGGA GATTCTGGGT ATGGTTGGAA CAGGCAGTCA 
GGTGGGCAGC AGAACAACTT CAAGGGACAA AGAGTATGCA AGTTCTTCCG CGAAAATGGA 
CATTGTCGGA AAGGAGCATC CTGCAATTAC CTCCACAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NERD ( NM_001335477.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001324854.1 Arabidopsis thaliana GW repeat- and PHD finger-containing protein NERD (NERD), mRNA.

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CloneID	OAb35707
Clone ID Related Accession (Same CDS sequence)	NM_001335477.1
Accession Version	NM_001335477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	GW repeat- and PHD finger-containing protein NERD
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGGATTCTG ATTCTGAGAG AGCTTCTCTG GAGTCGATTA AAGATAATTC TGAATGTGTT 
CATGTTTCCA ACGAACCTAA TCTCACGGCA ACATGCGTTG ATTCGTCTGT TGGAGAGGAA 
GGAGTTACGG ATGTAAATTC GTCCGCCGCT GTGTCGGAGC TCGTGCCGCC GGAGCAAGGA 
GAAGGTGCGC TTTTGAATTC CGTCCCTGAG ATCAGTGAAA GGGGGATTCC TGTTGATGTC 
GTTTCCTCGG TAGATGGAGG AGGAGAAGAA AATGCAGCTT TTAATATTCA GGAGATTGAT 
TCTGTTGGCG GTGACGCTGC GGCGGTGGAG GAGGTTCCTC TGAAATCGTC TTCGGTGGTT 
GGGGAAGGAA GAGAAGAGGA AGCTGGGGCT TCAATCGTCA AAGAAGAGGA TTTTGTAGCT 
GAAGCTAATT TGTCAGGAGA TAGGCTTGAG GAAAACAAAG AAGTTTCCAT GGAAGAAGAA 
CCTTCATCAC ATGAGCTCTC GGTTTGTGAG GTAAACGGAG TTGATTCTTT GAATGATGAG 
GAAAATAGAG AGGTAGGTGA ACAGATAGTT TGTGGAAGTA TGGGTGGTGA GGAGATAGAG 
AGTGATTTAG AGTCTAAAAA GGAAAAGGTA GATGTGATAG AGGAGGAAAC CACAGCGCAA 
GCTGCTTCCT TGGTTAATGC AATTGAAATT CCTGATGATA AAGAGGTAGC TTGCGTGGCT 
GGGTTTACGG AAATTTCCTC TCAAGATAAA GGGTTAGACG AATCAGGAAA TGGATTTTTA 
GATGAAGAGC CAGTGAAAGA GTTGCAAATA GGTGAAGGAG CTAAAGATCT GACAGATGGA 
GATGCAAAAG AAGGTGTGGA TGTTACAGAA GATGAAATGG ATATACAGGT ATTGAAGAAA 
TCTAAGGAGG AAGAAAAGGT TGATAGTACT ACTGAATTAG AGATAGAAAC TATGCGCCTA 
GAGGTGCATG ACGTGGCTAC TGAGATGTCC GACAAAACTG TTATATCATC AGCTGTTGTT 
ACTCAGTTTA CTGGGGAAAC AAGTAATGAT AAGGAAACTG TTATGGATGA TGTCAAAGAA 
GATGTTGACA AGGATTCAGA AGCTGGTAAA TCACTGGACA TACATGTACC AGAGGCTACA 
GAAGAAGTAG ACACAGATGT TAATTACGGT GTTGGGATAG AGAAAGAAGG CGATGGGGTG 
GGTGGTGCGG AAGAAGCAGG GCAGACAGTT GATTTGGAGG AAATTCGAGA AGAAAACCAA 
GAACTGTCTA AGGAACTGGC TCAAGTAGAT GAAACCAAGA TTTCTGAAAT GTCGGAAGTA 
ACGGAGACAA TGATCAAAGA TGAGGATCAG GAGAAAGATG ACAATATGAC TGATTTGGCG 
GAGGATGTGG AAAACCATAG AGATTCCTCA GTAGCTGATA TTGAGGAGGG AAGGGAAGAT 
CATGAAGATA TGGGAGTGAC AGAGACACAG AAGGAGACTG TTTTGGGTAA AGTAGACAGA 
ACAAAAATTG CTGAAGTGTC AGAAGAAACA GACACAAGGA TTGAAGATGA GGATCAGGAG 
AAAGATGATG AGATGACTGA TGTTGCGGAA GATGTGAAAA CGCATGGAGA CTCCTCAGTA 
GCTGATATTG AAGAGGGAAG GGAAAGTCAA GAAGAGATGA CAGAGACACA GGAGGACAGT 
GTTATGGCTG ATGAAGAGCC TGAGGAAGTT GAGGAAGAAA ATAAGAGTGC TGGAGGAAAG 
AGGAAACGGG GGAGAAATAC AAAGACAGTC AAAGGGACAG GAAAGAAGAA AGAGGAAGAT 
GTTTGTTTTA TGTGCTTTGA TGGGGGTGAT CTTGTCCTTT GTGACCGCAG GGGATGCACA 
AAAGCGTACC ACCCTTCCTG TGTAGATCGA GATGAGGCTT TCTTCCAGAC AAAGGGTAAA 
TGGAACTGTG GATGGCACCT ATGTAGCAAA TGTGAGAAAA CTGCAACGTA CTTGTGTTAT 
ACATGTATGT TTTCATTATG CAAGGGATGT GCCAAGGATG CTGTCTTCTT TTGCATCAGA 
GGTAATAAGG GTTTGTGTGA GACATGCATG GAGACAGTCA AGTTGATAGA AAGAAAACAG 
CAGGAAAAGG AGCCGGCGCA GTTGGATTTT AATGACAAGA CCAGCTGGGA ATATCTCTTT 
AAGGATTACT GGATTGATTT GAAAACCCAG CTATCTTTAA GTCCAGAGGA ACTTGATCAG 
GCTAAGAGGC CACTGAAAGG ACATGAGACC AATGCTAGCA AACAGGGAAC AGCCAGTGAG 
ACTGACTATG TTACTGATGG AGGATCAGAT TCAGATAGTT CTCCAAAAAA GAGGAAGACC 
AGGAGTCGAT CAAAGTCTGG TTCTGCTGAG AAAATCCTAT CATCAGGCGA TAAGAATTTG 
TCAGATGAAA CTATGGAGTG GGCTTCCAAG GAACTTTTGG ATCTGGTCGT GCACATGAGA 
CGTGGTGACA GATCTTTCTT ACCTATGTTA GAAGTACAGA CTCTCCTACT GGCTTATATA 
AAAAGATACA ATCTTCGTGA TCCCCGTCGT AAAAGCCAGG TTATCTGCGA CTCCAGGCTT 
CAGAATTTGT TCGGCAAATC ACATGTTGGA CACTTTGAGA TGTTAAATCT TCTTGACTCT 
CATTTTCTGA AAAAAGAGCA AAATCAGGCA GATGATATTC AAGGTGACAT AGTTGATACA 
GAAGAACCTA ATCATGTGGA TGTTGATGAA AACTTGGACC ATCCAGTCAA AAGTGGGAAG 
GATAAGAAAC GTAAAACACG CAAAAAAAAT GTCAGGAAAG GACGTCAATC TAATCTTGAT 
GATTTTGCTG CTGTTGATAT GCACAACATT AATTTGATAT ACTTAAGGCG GAGCTTGGTG 
GAAGATCTAC TTGAAGATTC TACAGCATTC GAAGAAAAAG TTGCTAGTGC ATTTGTTAGG 
TTAAGGATAT CTGGAAATCA AAAGCAAGAT TTATATCGAC TGGTTCAAGT TGTTGGCACA 
AGCAAAGCTC CTGAACCTTA TAAAGTTGGC AAAAAAACAA CAGACTATGT ACTTGAGATA 
TTGAACTTGG ACAAAACGGA AGTGATATCC ATTGACATTA TATCAAATCA AGATTTCACT 
GAGGATGAAT GCAAGCGTCT TAAGCAGAGC ATAAAATGTG GATTAATAAA CAGGCTGACA 
GTGGGTGATA TACAAGAAAA GGCCATTGCA CTGCAGGAAG TGAGAGTCAA AAACTTGCTG 
GAAGCAGAGA TCCTACGTTT CAGTCATTTG CGTGATCGTG CTAGTGACAT GGGCCGCAGA 
AAAGAGCTGC GAGAATGTGT TGAGAAACTG CAACTGTTAA AATCTCCAGA GGAACGGCAG 
CGCAGGCTAG AAGAAATTCC AGAAATACAT GCTGATCCCA AAATGGATCC AGATTGTGAA 
TCGGAAGATG AAGATGAAAA AGAAGAAAAG GAAAAAGAGA AACAACTGAG GCCGCGAAGC 
AGTAGCTTTA ACAGAAGAGG AAGAGATCCA ATATCTCCAC GAAAAGGAGG ATTTAGTTCC 
AATGAATCAT GGACTGGCAC AAGTAACTAC TCAAATACAA GTGCAAATCG TGAGTTAAGC 
AGAAGTTATT CTGGTAGAGG TTCTACTGGG AGAGGGGATT ATCTTGGTAG TTCTGACGAC 
AAGGTTAGTG ATAGTATGTG GACTTCAGCT AGAGAAAGAG AAGTGCAACC ATCTTTAGGC 
TCTGAGAAGC CAAGATCTGT TTCAATTCCT GAAACTCCTG CCAGAAGTTC TCGTGCTATT 
GCACCACCAG AATTATCTCC TAGGATTGCC TCTGAAATTT CAATGGCACC TCCTGCTGTT 
GTTTCGCAGC CTGTTCCCAA GTCAAATGAT TCAGAGAAAA TATGGCATTA CAAGGACCCA 
TCTGGTAAAG TTCAAGGACC TTTTTCGATG GCGCAGCTCC GTAAATGGAA CAACACTGGG 
TACTTCCCTG CTAAGTTGGA AATCTGGAAA GCAAATGAGT CACCGTTGGA CTCTGTTCTA 
CTGACTGATG CATTGGCTGG ACTATTTCAG AAGCAAACCC AGGCGGTGGA TAATAGCTAT 
ATGAAAGCTC AGGTAGCAGC TTTTTCTGGT CAATCAAGCC AATCTGAACC CAATTTAGGT 
TTTGCTGCTA GGATTGCTCC AACAACCATT GAGATTCCTA GGAATTCCCA AGATACTTGG 
AGTCAAGGTG GTAGTCTTCC ATCCCCTACT CCAAATCAAA TCACAACTCC AACAGCAAAA 
AGGCGAAATT TTGAGAGCAG ATGGTCTCCA ACCAAGCCAT CTCCACAGTC AGCTAATCAG 
TCGATGAATT ATTCAGTAGC ACAATCTGGA CAGAGTCAAA CTTCTAGGAT AGATATCCCT 
GTGGTTGTAA ATTCTGCTGG TGCTCTACAG CCTCAAACTT ACCCTATTCC AACACCAGAT 
CCGATCAACG TTTCTGTTAA TCACTCTGCG ACTCTTCATT CTCCAACACC AGCAGGTGGA 
AAGCAAAGCT GGGGGAGCAT GCAGACAGAT CATGGAGGAA GCAACACTCC CTCTTCTCAG 
AATAACTCTA CGTCTTATGG CACCCCATCT CCTAGTGTTC TCCCCTCACA GAGTCAACCA 
GGATTTCCTC CATCTGATTC TTGGAAGGTT GCAGTTCCAA GCCAACCGAA TGCTCAGGCT 
CAGGCCCAGT GGGGTATGAA CATGGTGAAC AACAACCAAA ACTCAGCACA ACCTCAGGCT 
CCAGCAAACC AGAACAGCAG CTGGGGTCAA GGGACTGTAA ATCCAAACAT GGGATGGGTT 
GGTCCTGCTC AAACTGGAGT GAATGTAAAT TGGGGTGGAT CTTCTGTTCC CTCAACTGTG 
CAAGGAATCA CTCATTCTGG CTGGGTCGCG CCTGTCCAAG GACAAACACA AGCTTATCCA 
AATCCAGGAT GGGGCCCAAC AGGCCACCCT CAATCTCAAT CCCAGTCTCA AGTTCAAGCT 
CAAGCGGGTA CTACGGGCTC GGGATGGATG CAACCAGGTC AAGGGATTCA GTCTGGAAAC 
AGTAATCAAA ATTGGGGAAC TCAAAATCAG ACAGCGATCC CAAGCGGAGG GTCAGGAGGG 
AACCAAGCCG GTTACTGGGG AAACCAACAA CAGAGCCAGA ATGGAGATTC TGGGTATGGT 
TGGAACAGGC AGTCAGGTGG GCAGCAGAAC AACTTCAAGG GACAAAGAGT ATGCAAGTTC 
TTCCGCGAAA ATGGACATTG TCGGAAAGGA GCATCCTGCA ATTACCTCCA CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001324854.1
CDS	203..5479
Translation	MDSDSERASLESIKDNSECVHVSNEPNLTATCVDSSVGEEGVTDVNSSAAVSELVPPEQGEGALLNSVPEISERGIPVDVVSSVDGGGEENAAFNIQEIDSVGGDAAAVEEVPLKSSSVVGEGREEEAGASIVKEEDFVAEANLSGDRLEENKEVSMEEEPSSHELSVCEVNGVDSLNDEENREVGEQIVCGSMGGEEIESDLESKKEKVDVIEEETTAQAASLVNAIEIPDDKEVACVAGFTEISSQDKGLDESGNGFLDEEPVKELQIGEGAKDLTDGDAKEGVDVTEDEMDIQVLKKSKEEEKVDSTTELEIETMRLEVHDVATEMSDKTVISSAVVTQFTGETSNDKETVMDDVKEDVDKDSEAGKSLDIHVPEATEEVDTDVNYGVGIEKEGDGVGGAEEAGQTVDLEEIREENQELSKELAQVDETKISEMSEVTETMIKDEDQEKDDNMTDLAEDVENHRDSSVADIEEGREDHEDMGVTETQKETVLGKVDRTKIAEVSEETDTRIEDEDQEKDDEMTDVAEDVKTHGDSSVADIEEGRESQEEMTETQEDSVMADEEPEEVEEENKSAGGKRKRGRNTKTVKGTGKKKEEDVCFMCFDGGDLVLCDRRGCTKAYHPSCVDRDEAFFQTKGKWNCGWHLCSKCEKTATYLCYTCMFSLCKGCAKDAVFFCIRGNKGLCETCMETVKLIERKQQEKEPAQLDFNDKTSWEYLFKDYWIDLKTQLSLSPEELDQAKRPLKGHETNASKQGTASETDYVTDGGSDSDSSPKKRKTRSRSKSGSAEKILSSGDKNLSDETMEWASKELLDLVVHMRRGDRSFLPMLEVQTLLLAYIKRYNLRDPRRKSQVICDSRLQNLFGKSHVGHFEMLNLLDSHFLKKEQNQADDIQGDIVDTEEPNHVDVDENLDHPVKSGKDKKRKTRKKNVRKGRQSNLDDFAAVDMHNINLIYLRRSLVEDLLEDSTAFEEKVASAFVRLRISGNQKQDLYRLVQVVGTSKAPEPYKVGKKTTDYVLEILNLDKTEVISIDIISNQDFTEDECKRLKQSIKCGLINRLTVGDIQEKAIALQEVRVKNLLEAEILRFSHLRDRASDMGRRKELRECVEKLQLLKSPEERQRRLEEIPEIHADPKMDPDCESEDEDEKEEKEKEKQLRPRSSSFNRRGRDPISPRKGGFSSNESWTGTSNYSNTSANRELSRSYSGRGSTGRGDYLGSSDDKVSDSMWTSAREREVQPSLGSEKPRSVSIPETPARSSRAIAPPELSPRIASEISMAPPAVVSQPVPKSNDSEKIWHYKDPSGKVQGPFSMAQLRKWNNTGYFPAKLEIWKANESPLDSVLLTDALAGLFQKQTQAVDNSYMKAQVAAFSGQSSQSEPNLGFAARIAPTTIEIPRNSQDTWSQGGSLPSPTPNQITTPTAKRRNFESRWSPTKPSPQSANQSMNYSVAQSGQSQTSRIDIPVVVNSAGALQPQTYPIPTPDPINVSVNHSATLHSPTPAGGKQSWGSMQTDHGGSNTPSSQNNSTSYGTPSPSVLPSQSQPGFPPSDSWKVAVPSQPNAQAQAQWGMNMVNNNQNSAQPQAPANQNSSWGQGTVNPNMGWVGPAQTGVNVNWGGSSVPSTVQGITHSGWVAPVQGQTQAYPNPGWGPTGHPQSQSQSQVQAQAGTTGSGWMQPGQGIQSGNSNQNWGTQNQTAIPSGGSGGNQAGYWGNQQQSQNGDSGYGWNRQSGGQQNNFKGQRVCKFFRENGHCRKGASCNYLHN

Target ORF information:

RefSeq Version	NM_001335477.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana GW repeat- and PHD finger-containing protein NERD (NERD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001335477.1

ORF Insert Sequence:

ATGGATTCTG ATTCTGAGAG AGCTTCTCTG GAGTCGATTA AAGATAATTC TGAATGTGTT 
CATGTTTCCA ACGAACCTAA TCTCACGGCA ACATGCGTTG ATTCGTCTGT TGGAGAGGAA 
GGAGTTACGG ATGTAAATTC GTCCGCCGCT GTGTCGGAGC TCGTGCCGCC GGAGCAAGGA 
GAAGGTGCGC TTTTGAATTC CGTCCCTGAG ATCAGTGAAA GGGGGATTCC TGTTGATGTC 
GTTTCCTCGG TAGATGGAGG AGGAGAAGAA AATGCAGCTT TTAATATTCA GGAGATTGAT 
TCTGTTGGCG GTGACGCTGC GGCGGTGGAG GAGGTTCCTC TGAAATCGTC TTCGGTGGTT 
GGGGAAGGAA GAGAAGAGGA AGCTGGGGCT TCAATCGTCA AAGAAGAGGA TTTTGTAGCT 
GAAGCTAATT TGTCAGGAGA TAGGCTTGAG GAAAACAAAG AAGTTTCCAT GGAAGAAGAA 
CCTTCATCAC ATGAGCTCTC GGTTTGTGAG GTAAACGGAG TTGATTCTTT GAATGATGAG 
GAAAATAGAG AGGTAGGTGA ACAGATAGTT TGTGGAAGTA TGGGTGGTGA GGAGATAGAG 
AGTGATTTAG AGTCTAAAAA GGAAAAGGTA GATGTGATAG AGGAGGAAAC CACAGCGCAA 
GCTGCTTCCT TGGTTAATGC AATTGAAATT CCTGATGATA AAGAGGTAGC TTGCGTGGCT 
GGGTTTACGG AAATTTCCTC TCAAGATAAA GGGTTAGACG AATCAGGAAA TGGATTTTTA 
GATGAAGAGC CAGTGAAAGA GTTGCAAATA GGTGAAGGAG CTAAAGATCT GACAGATGGA 
GATGCAAAAG AAGGTGTGGA TGTTACAGAA GATGAAATGG ATATACAGGT ATTGAAGAAA 
TCTAAGGAGG AAGAAAAGGT TGATAGTACT ACTGAATTAG AGATAGAAAC TATGCGCCTA 
GAGGTGCATG ACGTGGCTAC TGAGATGTCC GACAAAACTG TTATATCATC AGCTGTTGTT 
ACTCAGTTTA CTGGGGAAAC AAGTAATGAT AAGGAAACTG TTATGGATGA TGTCAAAGAA 
GATGTTGACA AGGATTCAGA AGCTGGTAAA TCACTGGACA TACATGTACC AGAGGCTACA 
GAAGAAGTAG ACACAGATGT TAATTACGGT GTTGGGATAG AGAAAGAAGG CGATGGGGTG 
GGTGGTGCGG AAGAAGCAGG GCAGACAGTT GATTTGGAGG AAATTCGAGA AGAAAACCAA 
GAACTGTCTA AGGAACTGGC TCAAGTAGAT GAAACCAAGA TTTCTGAAAT GTCGGAAGTA 
ACGGAGACAA TGATCAAAGA TGAGGATCAG GAGAAAGATG ACAATATGAC TGATTTGGCG 
GAGGATGTGG AAAACCATAG AGATTCCTCA GTAGCTGATA TTGAGGAGGG AAGGGAAGAT 
CATGAAGATA TGGGAGTGAC AGAGACACAG AAGGAGACTG TTTTGGGTAA AGTAGACAGA 
ACAAAAATTG CTGAAGTGTC AGAAGAAACA GACACAAGGA TTGAAGATGA GGATCAGGAG 
AAAGATGATG AGATGACTGA TGTTGCGGAA GATGTGAAAA CGCATGGAGA CTCCTCAGTA 
GCTGATATTG AAGAGGGAAG GGAAAGTCAA GAAGAGATGA CAGAGACACA GGAGGACAGT 
GTTATGGCTG ATGAAGAGCC TGAGGAAGTT GAGGAAGAAA ATAAGAGTGC TGGAGGAAAG 
AGGAAACGGG GGAGAAATAC AAAGACAGTC AAAGGGACAG GAAAGAAGAA AGAGGAAGAT 
GTTTGTTTTA TGTGCTTTGA TGGGGGTGAT CTTGTCCTTT GTGACCGCAG GGGATGCACA 
AAAGCGTACC ACCCTTCCTG TGTAGATCGA GATGAGGCTT TCTTCCAGAC AAAGGGTAAA 
TGGAACTGTG GATGGCACCT ATGTAGCAAA TGTGAGAAAA CTGCAACGTA CTTGTGTTAT 
ACATGTATGT TTTCATTATG CAAGGGATGT GCCAAGGATG CTGTCTTCTT TTGCATCAGA 
GGTAATAAGG GTTTGTGTGA GACATGCATG GAGACAGTCA AGTTGATAGA AAGAAAACAG 
CAGGAAAAGG AGCCGGCGCA GTTGGATTTT AATGACAAGA CCAGCTGGGA ATATCTCTTT 
AAGGATTACT GGATTGATTT GAAAACCCAG CTATCTTTAA GTCCAGAGGA ACTTGATCAG 
GCTAAGAGGC CACTGAAAGG ACATGAGACC AATGCTAGCA AACAGGGAAC AGCCAGTGAG 
ACTGACTATG TTACTGATGG AGGATCAGAT TCAGATAGTT CTCCAAAAAA GAGGAAGACC 
AGGAGTCGAT CAAAGTCTGG TTCTGCTGAG AAAATCCTAT CATCAGGCGA TAAGAATTTG 
TCAGATGAAA CTATGGAGTG GGCTTCCAAG GAACTTTTGG ATCTGGTCGT GCACATGAGA 
CGTGGTGACA GATCTTTCTT ACCTATGTTA GAAGTACAGA CTCTCCTACT GGCTTATATA 
AAAAGATACA ATCTTCGTGA TCCCCGTCGT AAAAGCCAGG TTATCTGCGA CTCCAGGCTT 
CAGAATTTGT TCGGCAAATC ACATGTTGGA CACTTTGAGA TGTTAAATCT TCTTGACTCT 
CATTTTCTGA AAAAAGAGCA AAATCAGGCA GATGATATTC AAGGTGACAT AGTTGATACA 
GAAGAACCTA ATCATGTGGA TGTTGATGAA AACTTGGACC ATCCAGTCAA AAGTGGGAAG 
GATAAGAAAC GTAAAACACG CAAAAAAAAT GTCAGGAAAG GACGTCAATC TAATCTTGAT 
GATTTTGCTG CTGTTGATAT GCACAACATT AATTTGATAT ACTTAAGGCG GAGCTTGGTG 
GAAGATCTAC TTGAAGATTC TACAGCATTC GAAGAAAAAG TTGCTAGTGC ATTTGTTAGG 
TTAAGGATAT CTGGAAATCA AAAGCAAGAT TTATATCGAC TGGTTCAAGT TGTTGGCACA 
AGCAAAGCTC CTGAACCTTA TAAAGTTGGC AAAAAAACAA CAGACTATGT ACTTGAGATA 
TTGAACTTGG ACAAAACGGA AGTGATATCC ATTGACATTA TATCAAATCA AGATTTCACT 
GAGGATGAAT GCAAGCGTCT TAAGCAGAGC ATAAAATGTG GATTAATAAA CAGGCTGACA 
GTGGGTGATA TACAAGAAAA GGCCATTGCA CTGCAGGAAG TGAGAGTCAA AAACTTGCTG 
GAAGCAGAGA TCCTACGTTT CAGTCATTTG CGTGATCGTG CTAGTGACAT GGGCCGCAGA 
AAAGAGCTGC GAGAATGTGT TGAGAAACTG CAACTGTTAA AATCTCCAGA GGAACGGCAG 
CGCAGGCTAG AAGAAATTCC AGAAATACAT GCTGATCCCA AAATGGATCC AGATTGTGAA 
TCGGAAGATG AAGATGAAAA AGAAGAAAAG GAAAAAGAGA AACAACTGAG GCCGCGAAGC 
AGTAGCTTTA ACAGAAGAGG AAGAGATCCA ATATCTCCAC GAAAAGGAGG ATTTAGTTCC 
AATGAATCAT GGACTGGCAC AAGTAACTAC TCAAATACAA GTGCAAATCG TGAGTTAAGC 
AGAAGTTATT CTGGTAGAGG TTCTACTGGG AGAGGGGATT ATCTTGGTAG TTCTGACGAC 
AAGGTTAGTG ATAGTATGTG GACTTCAGCT AGAGAAAGAG AAGTGCAACC ATCTTTAGGC 
TCTGAGAAGC CAAGATCTGT TTCAATTCCT GAAACTCCTG CCAGAAGTTC TCGTGCTATT 
GCACCACCAG AATTATCTCC TAGGATTGCC TCTGAAATTT CAATGGCACC TCCTGCTGTT 
GTTTCGCAGC CTGTTCCCAA GTCAAATGAT TCAGAGAAAA TATGGCATTA CAAGGACCCA 
TCTGGTAAAG TTCAAGGACC TTTTTCGATG GCGCAGCTCC GTAAATGGAA CAACACTGGG 
TACTTCCCTG CTAAGTTGGA AATCTGGAAA GCAAATGAGT CACCGTTGGA CTCTGTTCTA 
CTGACTGATG CATTGGCTGG ACTATTTCAG AAGCAAACCC AGGCGGTGGA TAATAGCTAT 
ATGAAAGCTC AGGTAGCAGC TTTTTCTGGT CAATCAAGCC AATCTGAACC CAATTTAGGT 
TTTGCTGCTA GGATTGCTCC AACAACCATT GAGATTCCTA GGAATTCCCA AGATACTTGG 
AGTCAAGGTG GTAGTCTTCC ATCCCCTACT CCAAATCAAA TCACAACTCC AACAGCAAAA 
AGGCGAAATT TTGAGAGCAG ATGGTCTCCA ACCAAGCCAT CTCCACAGTC AGCTAATCAG 
TCGATGAATT ATTCAGTAGC ACAATCTGGA CAGAGTCAAA CTTCTAGGAT AGATATCCCT 
GTGGTTGTAA ATTCTGCTGG TGCTCTACAG CCTCAAACTT ACCCTATTCC AACACCAGAT 
CCGATCAACG TTTCTGTTAA TCACTCTGCG ACTCTTCATT CTCCAACACC AGCAGGTGGA 
AAGCAAAGCT GGGGGAGCAT GCAGACAGAT CATGGAGGAA GCAACACTCC CTCTTCTCAG 
AATAACTCTA CGTCTTATGG CACCCCATCT CCTAGTGTTC TCCCCTCACA GAGTCAACCA 
GGATTTCCTC CATCTGATTC TTGGAAGGTT GCAGTTCCAA GCCAACCGAA TGCTCAGGCT 
CAGGCCCAGT GGGGTATGAA CATGGTGAAC AACAACCAAA ACTCAGCACA ACCTCAGGCT 
CCAGCAAACC AGAACAGCAG CTGGGGTCAA GGGACTGTAA ATCCAAACAT GGGATGGGTT 
GGTCCTGCTC AAACTGGAGT GAATGTAAAT TGGGGTGGAT CTTCTGTTCC CTCAACTGTG 
CAAGGAATCA CTCATTCTGG CTGGGTCGCG CCTGTCCAAG GACAAACACA AGCTTATCCA 
AATCCAGGAT GGGGCCCAAC AGGCCACCCT CAATCTCAAT CCCAGTCTCA AGTTCAAGCT 
CAAGCGGGTA CTACGGGCTC GGGATGGATG CAACCAGGTC AAGGGATTCA GTCTGGAAAC 
AGTAATCAAA ATTGGGGAAC TCAAAATCAG ACAGCGATCC CAAGCGGAGG GTCAGGAGGG 
AACCAAGCCG GTTACTGGGG AAACCAACAA CAGAGCCAGA ATGGAGATTC TGGGTATGGT 
TGGAACAGGC AGTCAGGTGG GCAGCAGAAC AACTTCAAGG GACAAAGAGT ATGCAAGTTC 
TTCCGCGAAA ATGGACATTG TCGGAAAGGA GCATCCTGCA ATTACCTCCA CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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NERD cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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