LOC100865528 cDNA ORF clone, Apis florea(little honeybee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100865528
Entrez Gene ID	100865528
Full Name	putative uncharacterized protein DDB_G0282499
Gene Type	protein-coding
Organism	Apis florea(little honeybee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012491830.1	XP_012347284.1	LOW QUALITY PROTEIN: putative uncharacterized protein DDB_G0282499
XM_012491830.2	XP_012347284.2	putative uncharacterized protein DDB_G0282499

Pathways from BioSystems
Gene Ontology

The following LOC100865528 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100865528 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp183825	XM_012491830.2 Latest version!	Apis florea putative uncharacterized protein DDB_G0282499 (LOC100865528), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAp128610	XM_012491830.1 Latest version!	Apis florea putative uncharacterized protein DDB_G0282499 (LOC100865528), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100865528 ( XM_012491830.2 ) cDNA ORF clone, Apis florea(little honeybee) -> XP_012347284.2 Apis florea putative uncharacterized protein DDB_G0282499 (LOC100865528), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAp183825
Clone ID Related Accession (Same CDS sequence)	XM_012491830.2
Accession Version	XM_012491830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Apis florea(little honeybee)
Product	putative uncharacterized protein DDB_G0282499
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003789385.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 23, 2019 this sequence version replaced XM_012491830.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis florea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGAT ATGCCTGGGT TACATTAGCT ACCAATGATG CTTATTCCTT GGGAGCTTTA 
GTTTTGGCAC ATTCTTTACA TCGAGTTGGT ACCAAATATG AATTGGCTGT TTTGATTACC 
CCTGGAGTAA CGCAGATAAT GAGGGAAAAA CTTTCAGGAA TCTTTTCTGT AGTAATGGAA 
GTAAATGTAC TTGATTCAAA AGATGAAGCT AATTTGGCAC TTTTAGCTAG ACCAGAATTA 
GGTATTACAT TCACTAAACT TCATTGCTGG AGATTGACTC AATATGAAAA GTGTGTGTTT 
CTTGATGCAG ATACACTTGT TGTCAGAAAT TGTGATGAAC TATTTGAAAG AGAAGAACTA 
TCTGCTGCTC CTGATGTGGG TTGGCCTGAT TGTTTCAATT CTGGTGTATT TGTATACAGA 
CCATCCCAAC AAACTTTTGC TTCTATTACT GCATTTGCTG CTGCTAAAGG TTCATTTGAT 
GGTGGTGACC AAGGATTATT GAATATGTAT TTCAGTGATT GGGCTAGAAA AGATATTTCT 
AAACATTTGC CATTTATTTA TAATATGTGT TCTACTGCTA CATACTCTTA TCTACCTGCA 
TTCAAGCAGT TTGGAGATGA TGTTAGAATA ATTCATTTTA TTGGTATTAC AAAACCATGG 
TTACAGTATT TTGACACATT AACTGGTGTT GTTCAACCAC CAATGGGTTC TACACACTTG 
CAACCATTAT TACAATTATG GTGGAATATA TTTTGTGAAA AAGTGCATTC TCAATTATCA 
CCTGTAATGG CTACCAGCAC ATTGGCACCA ATTTGGCACG AATTTTCTCC TATGTTTTTT 
GAATCCTATG TTCCAAAAAA TCCCATTTGT ACAGACATAC AAGACAAAAC ACAAAATGAC 
ATATATCTAG AACTACCAGA TTTCTCAGAA TTTCAAGATC CATGGGAAAA TTATTATGTA 
CAAAATAATT CAGTTATTTA TAAAACAGAA AATAATGAAA GACAACAGTA TTATTCTGTA 
AACAACTCTT CACCTGTATC TGTATTAAAT GATCAATTTA GTCACATAAA GTCTGTTCAA 
CAGACTCATG AATCATCTCC AAAAAAAAAT AGGGAATTAA TAGGGAATCA TAATCAACAA 
TATAATGAAC ATATTTTTTC TTTGCATACT CAATATCATC AAAATCAAGA ATCTAATCAA 
TTTATCAATA AAATTGAAAA ACGACATTCA TTTTCTCATT CTGATAATAC AACTATTATA 
AATATACAAC CAAATTATTT CCAACTTGAA CACAATATAA AAAAATATAT ACCTGTACAA 
AATCATCATT CGAATGAATA TCAACATGAA TCTGTCAACT ATCAATATAA TTCTCAAAAA 
ACTTGCAATA ATAATAATAA TAATAATAAT AATAATAATA ATAATAATAA TAATAATAAG 
AACAACAACA ATAATAATAA CAATAATAAT AATAATAATA TATTACCAAA AGAAACAAAA 
CAACAATATA TTAATTATGA ACATACTTAT CAACATTCTT CTAATAATAT TAATAAATCA 
TTGTCAGAAG AAGTAAAACA TCTACATAAT AAACTTTATA GAGATCATCA TGATCATTAC 
TATCAAATGC TCGATAATAT AAATAATAAA CATTTTCAAC ATCATGAACA AAAAAATGAA 
ACTTGCAATT CTCTAGATGT CAACATTATT CATGATCAAC ATTATATTAT GCTAAATAAT 
ATAACTTATC AGAATAATGT TTTAATTAAG CAAAATATTG CACAAAATGA AAATATGAAC 
GTTACAGACA AACGAACATT AATATCTACT AATCCTTATC CTGATTCCAA ACCTTGTATA 
GATTTCCACA ATGTAGCAAC GCAATCTGAT CCACATATAA TGGATGATCT AAATAATTCA 
AATGCTGGTC TTGCTGGTGC CTTGGCTCAA ATGACATTAG GTGAACCCAG AAGCTCTGAA 
CAAATTGCAT TAGAAGAACA TATGCGGAAA CAAAGTTGGG AACAAGGTCA AATAGACTAT 
ATGGGTCGAG ATAGTTTTGA TAATATTTGG AAAAAAATTT GTGAAACTCT TGCTCTTGCA 
CCTCCACGAT TACCATCTCC TCCTAAAGAA ATACAAAAGT CTGTCAAAAC TACAATTGAT 
GAAACTGATG AATTTATTAA ATCTGCTGAA ATTACTCAAT TTATCAAGTC TACAGATGAA 
GTGGATAAAA GAACCGCTCA AACAAACAGT CATACTGAAG AATCTCTAGT TAATAATGTT 
CCTACCACAA ATGTACCTAA ATTATTGGAA AATCAAAAGA GTGAAGATGA TATTTCAAGA 
AAATCACGAA ATAATACATT TATAGATTTT CCTGCAACAA ACCTTTCTGT ACAAACATCT 
CATGAAGAAA TAACAGAACT TAATACAACT GAATTATTAA ATAAAATTTC ACAACAGACT 
AGTGTACCTA TAGTATCGTC TGATTTAAAT CAATCTTGTT CAATTGGAGA ACAGAAAATA 
ATTATTTCAA CAAAAGAATT AAAAAACAAA TTACAGAATA AAGATCTTAA AATTACCACA 
GATATTCATC AAATGAATCA ACCAGATATT AGAGATGAAA AATATGCAAA AGAATTATTA 
ACTGCATCTG AAATTTTAAC AGTTAGCCCT GTTCCGATTC AAGTTGCAGA ATCTGTATTA 
CAAATGGAAC CAAAAGAATC TGTGTTAGAT TCTCAAATAA TATCGCAAGA AGTTCTTTCT 
TCTATAATTA CACCAGTTCA TTTAACAGAA ACAGAAAATA AAATACATAT TAATGATTCA 
ATAGAGTTCA TAGAAAGTAA AGAGAAATTA AATTTAGATA ATATCACAAA TGTAAAAAAT 
ATTGATGCTA TCAATAAATT AAGCAAATTG TCAATAAAAC CAAAAGAAAT CTCATTGACA 
ATAGAACAAA TTCAACCTCA ATTTGAAGAA GAAATACCTA CATTATCTTC AAATAACTTT 
GAAATACCAG AGAAATTAAT GCAAAAACAA ACAGAACAAC AAAGAAAAAC AAGTGAATAT 
TGTATAAAAG AAGCAATGAA ATTAAATAAA ACAGAATCAG AATATGAAAC AAATAAAACA 
TTAAACAAAA CTCCAGTTAG ACCTTTAAGA ATAAAAGAAT TAAATTCTTC ACCTTCAACA 
AGTATTAAAG AAACTGGACA ATTTGATTCA ACAAGTCAAA AGAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012347284.2
CDS	160..3387
Translation	MGGYAWVTLATNDAYSLGALVLAHSLHRVGTKYELAVLITPGVTQIMREKLSGIFSVVMEVNVLDSKDEANLALLARPELGITFTKLHCWRLTQYEKCVFLDADTLVVRNCDELFEREELSAAPDVGWPDCFNSGVFVYRPSQQTFASITAFAAAKGSFDGGDQGLLNMYFSDWARKDISKHLPFIYNMCSTATYSYLPAFKQFGDDVRIIHFIGITKPWLQYFDTLTGVVQPPMGSTHLQPLLQLWWNIFCEKVHSQLSPVMATSTLAPIWHEFSPMFFESYVPKNPICTDIQDKTQNDIYLELPDFSEFQDPWENYYVQNNSVIYKTENNERQQYYSVNNSSPVSVLNDQFSHIKSVQQTHESSPKKNRELIGNHNQQYNEHIFSLHTQYHQNQESNQFINKIEKRHSFSHSDNTTIINIQPNYFQLEHNIKKYIPVQNHHSNEYQHESVNYQYNSQKTCNNNNNNNNNNNNNNNNNKNNNNNNNNNNNNNILPKETKQQYINYEHTYQHSSNNINKSLSEEVKHLHNKLYRDHHDHYYQMLDNINNKHFQHHEQKNETCNSLDVNIIHDQHYIMLNNITYQNNVLIKQNIAQNENMNVTDKRTLISTNPYPDSKPCIDFHNVATQSDPHIMDDLNNSNAGLAGALAQMTLGEPRSSEQIALEEHMRKQSWEQGQIDYMGRDSFDNIWKKICETLALAPPRLPSPPKEIQKSVKTTIDETDEFIKSAEITQFIKSTDEVDKRTAQTNSHTEESLVNNVPTTNVPKLLENQKSEDDISRKSRNNTFIDFPATNLSVQTSHEEITELNTTELLNKISQQTSVPIVSSDLNQSCSIGEQKIIISTKELKNKLQNKDLKITTDIHQMNQPDIRDEKYAKELLTASEILTVSPVPIQVAESVLQMEPKESVLDSQIISQEVLSSIITPVHLTETENKIHINDSIEFIESKEKLNLDNITNVKNIDAINKLSKLSIKPKEISLTIEQIQPQFEEEIPTLSSNNFEIPEKLMQKQTEQQRKTSEYCIKEAMKLNKTESEYETNKTLNKTPVRPLRIKELNSSPSTSIKETGQFDSTSQKN

Target ORF information:

RefSeq Version	XM_012491830.2
Organism	Apis florea(little honeybee)
Definition	Apis florea putative uncharacterized protein DDB_G0282499 (LOC100865528), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012491830.2

ORF Insert Sequence:

ATGGGTGGAT ATGCCTGGGT TACATTAGCT ACCAATGATG CTTATTCCTT GGGAGCTTTA 
GTTTTGGCAC ATTCTTTACA TCGAGTTGGT ACCAAATATG AATTGGCTGT TTTGATTACC 
CCTGGAGTAA CGCAGATAAT GAGGGAAAAA CTTTCAGGAA TCTTTTCTGT AGTAATGGAA 
GTAAATGTAC TTGATTCAAA AGATGAAGCT AATTTGGCAC TTTTAGCTAG ACCAGAATTA 
GGTATTACAT TCACTAAACT TCATTGCTGG AGATTGACTC AATATGAAAA GTGTGTGTTT 
CTTGATGCAG ATACACTTGT TGTCAGAAAT TGTGATGAAC TATTTGAAAG AGAAGAACTA 
TCTGCTGCTC CTGATGTGGG TTGGCCTGAT TGTTTCAATT CTGGTGTATT TGTATACAGA 
CCATCCCAAC AAACTTTTGC TTCTATTACT GCATTTGCTG CTGCTAAAGG TTCATTTGAT 
GGTGGTGACC AAGGATTATT GAATATGTAT TTCAGTGATT GGGCTAGAAA AGATATTTCT 
AAACATTTGC CATTTATTTA TAATATGTGT TCTACTGCTA CATACTCTTA TCTACCTGCA 
TTCAAGCAGT TTGGAGATGA TGTTAGAATA ATTCATTTTA TTGGTATTAC AAAACCATGG 
TTACAGTATT TTGACACATT AACTGGTGTT GTTCAACCAC CAATGGGTTC TACACACTTG 
CAACCATTAT TACAATTATG GTGGAATATA TTTTGTGAAA AAGTGCATTC TCAATTATCA 
CCTGTAATGG CTACCAGCAC ATTGGCACCA ATTTGGCACG AATTTTCTCC TATGTTTTTT 
GAATCCTATG TTCCAAAAAA TCCCATTTGT ACAGACATAC AAGACAAAAC ACAAAATGAC 
ATATATCTAG AACTACCAGA TTTCTCAGAA TTTCAAGATC CATGGGAAAA TTATTATGTA 
CAAAATAATT CAGTTATTTA TAAAACAGAA AATAATGAAA GACAACAGTA TTATTCTGTA 
AACAACTCTT CACCTGTATC TGTATTAAAT GATCAATTTA GTCACATAAA GTCTGTTCAA 
CAGACTCATG AATCATCTCC AAAAAAAAAT AGGGAATTAA TAGGGAATCA TAATCAACAA 
TATAATGAAC ATATTTTTTC TTTGCATACT CAATATCATC AAAATCAAGA ATCTAATCAA 
TTTATCAATA AAATTGAAAA ACGACATTCA TTTTCTCATT CTGATAATAC AACTATTATA 
AATATACAAC CAAATTATTT CCAACTTGAA CACAATATAA AAAAATATAT ACCTGTACAA 
AATCATCATT CGAATGAATA TCAACATGAA TCTGTCAACT ATCAATATAA TTCTCAAAAA 
ACTTGCAATA ATAATAATAA TAATAATAAT AATAATAATA ATAATAATAA TAATAATAAG 
AACAACAACA ATAATAATAA CAATAATAAT AATAATAATA TATTACCAAA AGAAACAAAA 
CAACAATATA TTAATTATGA ACATACTTAT CAACATTCTT CTAATAATAT TAATAAATCA 
TTGTCAGAAG AAGTAAAACA TCTACATAAT AAACTTTATA GAGATCATCA TGATCATTAC 
TATCAAATGC TCGATAATAT AAATAATAAA CATTTTCAAC ATCATGAACA AAAAAATGAA 
ACTTGCAATT CTCTAGATGT CAACATTATT CATGATCAAC ATTATATTAT GCTAAATAAT 
ATAACTTATC AGAATAATGT TTTAATTAAG CAAAATATTG CACAAAATGA AAATATGAAC 
GTTACAGACA AACGAACATT AATATCTACT AATCCTTATC CTGATTCCAA ACCTTGTATA 
GATTTCCACA ATGTAGCAAC GCAATCTGAT CCACATATAA TGGATGATCT AAATAATTCA 
AATGCTGGTC TTGCTGGTGC CTTGGCTCAA ATGACATTAG GTGAACCCAG AAGCTCTGAA 
CAAATTGCAT TAGAAGAACA TATGCGGAAA CAAAGTTGGG AACAAGGTCA AATAGACTAT 
ATGGGTCGAG ATAGTTTTGA TAATATTTGG AAAAAAATTT GTGAAACTCT TGCTCTTGCA 
CCTCCACGAT TACCATCTCC TCCTAAAGAA ATACAAAAGT CTGTCAAAAC TACAATTGAT 
GAAACTGATG AATTTATTAA ATCTGCTGAA ATTACTCAAT TTATCAAGTC TACAGATGAA 
GTGGATAAAA GAACCGCTCA AACAAACAGT CATACTGAAG AATCTCTAGT TAATAATGTT 
CCTACCACAA ATGTACCTAA ATTATTGGAA AATCAAAAGA GTGAAGATGA TATTTCAAGA 
AAATCACGAA ATAATACATT TATAGATTTT CCTGCAACAA ACCTTTCTGT ACAAACATCT 
CATGAAGAAA TAACAGAACT TAATACAACT GAATTATTAA ATAAAATTTC ACAACAGACT 
AGTGTACCTA TAGTATCGTC TGATTTAAAT CAATCTTGTT CAATTGGAGA ACAGAAAATA 
ATTATTTCAA CAAAAGAATT AAAAAACAAA TTACAGAATA AAGATCTTAA AATTACCACA 
GATATTCATC AAATGAATCA ACCAGATATT AGAGATGAAA AATATGCAAA AGAATTATTA 
ACTGCATCTG AAATTTTAAC AGTTAGCCCT GTTCCGATTC AAGTTGCAGA ATCTGTATTA 
CAAATGGAAC CAAAAGAATC TGTGTTAGAT TCTCAAATAA TATCGCAAGA AGTTCTTTCT 
TCTATAATTA CACCAGTTCA TTTAACAGAA ACAGAAAATA AAATACATAT TAATGATTCA 
ATAGAGTTCA TAGAAAGTAA AGAGAAATTA AATTTAGATA ATATCACAAA TGTAAAAAAT 
ATTGATGCTA TCAATAAATT AAGCAAATTG TCAATAAAAC CAAAAGAAAT CTCATTGACA 
ATAGAACAAA TTCAACCTCA ATTTGAAGAA GAAATACCTA CATTATCTTC AAATAACTTT 
GAAATACCAG AGAAATTAAT GCAAAAACAA ACAGAACAAC AAAGAAAAAC AAGTGAATAT 
TGTATAAAAG AAGCAATGAA ATTAAATAAA ACAGAATCAG AATATGAAAC AAATAAAACA 
TTAAACAAAA CTCCAGTTAG ACCTTTAAGA ATAAAAGAAT TAAATTCTTC ACCTTCAACA 
AGTATTAAAG AAACTGGACA ATTTGATTCA ACAAGTCAAA AGAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100865528 ( XM_012491830.1 ) cDNA ORF clone, Apis florea(little honeybee) -> XP_012347284.1 Apis florea putative uncharacterized protein DDB_G0282499 (LOC100865528), mRNA.

Price & Availability↑

CloneID	OAp128610
Clone ID Related Accession (Same CDS sequence)	XM_012491830.1
Accession Version	XM_012491830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-11
Organism	Apis florea(little honeybee)
Product	LOW QUALITY PROTEIN: putative uncharacterized protein DDB_G0282499
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003789385.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis florea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGGTGGAT ATGCCTGGGT TACATTAGCT ACCAATGATG CTTATTCCTT GGGAGCTTTA 
GTTTTGGCAC ATTCTTTACA TCGAGTTGGT ACCAAATATG AATTGGCTGT TTTGATTACC 
CCTGGAGTAA CGCAGATAAT GAGGGAAAAA CTTTCAGGAA TCTTTTCTGT AGTAATGGAA 
GTAAATGTAC TTGATTCAAA AGATGAAGCT AATTTGGCAC TTTTAGCTAG ACCAGAATTA 
GGTATTACAT TCACTAAACT TCATTGCTGG AGATTGACTC AATATGAAAA GTGTGTGTTT 
CTTGATGCAG ATACACTTGT TGTCAGAAAT TGTGATGAAC TATTTGAAAG AGAAGAACTA 
TCTGCTGCTC CTGATGTGGG TTGGCCTGAT TGTTTCAATT CTGGTGTATT TGTATACAGA 
CCATCCCAAC AAACTTTTGC TTCTATTACT GCATTTGCTG CTGCTAAAGG TTCATTTGAT 
GGTGGTGACC AAGGATTATT GAATATGTAT TTCAGTGATT GGGCTAGAAA AGATATTTCT 
AAACATTTGC CATTTATTTA TAATATGTGT TCTACTGCTA CATACTCTTA TCTACCTGCA 
TTCAAGCAGT TTGGAGATGA TGTTAGAATA ATTCATTTTA TTGGTATTAC AAAACCATGG 
TTACAGTATT TTGACACATT AACTGGTGTT GTTCAACCAC CAATGGGTTC TACACACTTG 
CAACCATTAT TACAATTATG GTGGAATATA TTTTGTGAAA AAGTGCATTC TCAATTATCA 
CCTGTAATGG CTACCAGCAC ATTGGCACCA ATTTGGCACG AATTTTCTCC TATGTTTTTT 
GAATCCTATG TTCCAAAAAA TCCCATTTGT ACAGACATAC AAGACAAAAC ACAAAATGAC 
ATATATCTAG AACTACCAGA TTTCTCAGAA TTTCAAGATC CATGGGAAAA TTATTATGTA 
CAAAATAATT CAGTTATTTA TAAAACAGAA AATAATGAAA GACAACAGTA TTATTCTGTA 
AACAACTCTT CACCTGTATC TGTATTAAAT GATCAATTTA GTCACATAAA GTCTGTTCAA 
CAGACTCATG AATCATCTCC AAAAAAAAAT AGGGAATTAA TAGGGAATCA TAATCAACAA 
TATAATGAAC ATATTTTTTC TTTGCATACT CAATATCATC AAAATCAAGA ATCTAATCAA 
TTTATCAATA AAATTGAAAA ACGACATTCA TTTTCTCATT CTGATAATAC AACTATTATA 
AATATACAAC CAAATTATTT CCAACTTGAA CACAATATAA AAAAATATAT ACCTGTACAA 
AATCATCATT CGAATGAATA TCAACATGAA TCTGTCAACT ATCAATATAA TTCTCAAAAA 
ACTTGCAATA ATAATAATAA TAATAATAAT AATAATAATA ATAATAATAA TAATAATAAG 
AACAACAACA ATAATAATAA CAATAATAAT AATAATAATA TATTACCAAA AGAAACAAAA 
CAACAATATA TTAATTATGA ACATACTTAT CAACATTCTT CTAATAATAT TAATAAATCA 
TTGTCAGAAG AAGTAAAACA TCTACATAAT AAACTTTATA GAGATCATCA TGATCATTAC 
TATCAAATGC TCGATAATAT AAATAATAAA CATTTTCAAC ATCATGAACA AAAAAATGAA 
ACTTGCAATT CTCTAGATGT CAACATTATT CATGATCAAC ATTATATTAT GCTAAATAAT 
ATAACTTATC AGAATAATGT TTTAATTAAG CAAAATATTG CACAAAATGA AAATATGAAC 
GTTACAGACA AACGAACATT AATATCTACT AATCCTTATC CTGATTCCAA ACCTTGTATA 
GATTTCCACA ATGTAGCAAC GCAATCTGAT CCACATATAA TGGATGATCT AAATAATTCA 
AATGCTGGTC TTGCTGGTGC CTTGGCTCAA ATGACATTAG GTGAACCCAG AAGCTCTGAA 
CAAATTGCAT TAGAAGAACA TATGCGGAAA CAAAGTTGGG AACAAGGTCA AATAGACTAT 
ATGGGTCGAG ATAGTTTTGA TAATATTTGG AAAAAAATTT GTGAAACTCT TGCTCTTGCA 
CCTCCACGAT TACCATCTCC TCCTAAAGAA ATACAAAAGT CTGTCAAAAC TACAATTGAT 
GAAACTGATG AATTTATTAA ATCTGCTGAA ATTACTCAAT TTATCAAGTC TACAGATGAA 
GTGGATAAAA GAACCGCTCA AACAAACAGT CATACTGAAG AATCTCTAGT TAATAATGTT 
CCTACCACAA ATGTACCTAA ATTATTGGAA AATCAAAAGA GTGAAGATGA TATTTCAAGA 
AAATCACGAA ATAATACATT TATAGATTTT CCTGCAACAA ACCTTTCTGT ACAAACATCT 
CATGAAGAAA TAACAGAACT TAATACAACT GAATTATTAA ATAAAATTTC ACAACAGACT 
AGTGTACCTA TAGTATCGTC TGATTTAAAT CAATCTTGTT CAATTGGAGA ACAGAAAATA 
ATTATTTCAA CAAAAGAATT AAAAAACAAA TTACAGAATA AAGATCTTAA AATTACCACA 
GATATTCATC AAATGAATCA ACCAGATATT AGAGATGAAA AATATGCAAA AGAATTATTA 
ACTGCATCTG AAATTTTAAC AGTTAGCCCT GTTCCGATTC AAGTTGCAGA ATCTGTATTA 
CAAATGGAAC CAAAAGAATC TGTGTTAGAT TCTCAAATAA TATCGCAAGA AGTTCTTTCT 
TCTATAATTA CACCAGTTCA TTTAACAGAA ACAGAAAATA AAATACATAT TAATGATTCA 
ATAGAGTTCA TAGAAAGTAA AGAGAAATTA AATTTAGATA ATATCACAAA TGTAAAAAAT 
ATTGATGCTA TCAATAAATT AAGCAAATTG TCAATAAAAC CAAAAGAAAT CTCATTGACA 
ATAGAACAAA TTCAACCTCA ATTTGAAGAA GAAATACCTA CATTATCTTC AAATAACTTT 
GAAATACCAG AGAAATTAAT GCAAAAACAA ACAGAACAAC AAAGAAAAAC AAGTGAATAT 
TGTATAAAAG AAGCAATGAA ATTAAATAAA ACAGAATCAG AATATGAAAC AAATAAAACA 
TTAAACAAAA CTCCAGTTAG ACCTTTAAGA ATAAAAGAAT TAAATTCTTC ACCTTCAACA 
AGTATTAAAG AAACTGGACA ATTTGATTCA ACAAGTCAAA AGNAATTAAT AAAAAAGATA 
AAAAAATCTA CAGAAAAATC AGTATCAATA GGAGAACATG TAGAAGCAAT TGATGATGAT 
ACTGAAAAAA AAATAACAAA GAAGATAGTA AAAAAGAAAA AATTAAAATC AGAAGAAGGA 
CTAGATGATA ATATAGTAGA CAGCAGTTCT TTTAATAAAC AAAAAAAGAC TTTAAAGTTG 
TTAAAAAAAG GTATAAAATC ATCACAAAAA ACTTCTACTG ATGCTACTAT TTCTGAAATT 
CCAACATCTA GCACTTCTGA TTTAACTCCA GTTCCACCTA AAAGAAAAGT GAAAACTATA 
GCTGCAAAAT TGGTTATTAA AAAATCAGAT ATAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012347284.1
CDS	163..3741
Translation	MGGYAWVTLATNDAYSLGALVLAHSLHRVGTKYELAVLITPGVTQIMREKLSGIFSVVMEVNVLDSKDEANLALLARPELGITFTKLHCWRLTQYEKCVFLDADTLVVRNCDELFEREELSAAPDVGWPDCFNSGVFVYRPSQQTFASITAFAAAKGSFDGGDQGLLNMYFSDWARKDISKHLPFIYNMCSTATYSYLPAFKQFGDDVRIIHFIGITKPWLQYFDTLTGVVQPPMGSTHLQPLLQLWWNIFCEKVHSQLSPVMATSTLAPIWHEFSPMFFESYVPKNPICTDIQDKTQNDIYLELPDFSEFQDPWENYYVQNNSVIYKTENNERQQYYSVNNSSPVSVLNDQFSHIKSVQQTHESSPKKNRELIGNHNQQYNEHIFSLHTQYHQNQESNQFINKIEKRHSFSHSDNTTIINIQPNYFQLEHNIKKYIPVQNHHSNEYQHESVNYQYNSQKTCNNNNNNNNNNNNNNNNNKNNNNNNNNNNNNNILPKETKQQYINYEHTYQHSSNNINKSLSEEVKHLHNKLYRDHHDHYYQMLDNINNKHFQHHEQKNETCNSLDVNIIHDQHYIMLNNITYQNNVLIKQNIAQNENMNVTDKRTLISTNPYPDSKPCIDFHNVATQSDPHIMDDLNNSNAGLAGALAQMTLGEPRSSEQIALEEHMRKQSWEQGQIDYMGRDSFDNIWKKICETLALAPPRLPSPPKEIQKSVKTTIDETDEFIKSAEITQFIKSTDEVDKRTAQTNSHTEESLVNNVPTTNVPKLLENQKSEDDISRKSRNNTFIDFPATNLSVQTSHEEITELNTTELLNKISQQTSVPIVSSDLNQSCSIGEQKIIISTKELKNKLQNKDLKITTDIHQMNQPDIRDEKYAKELLTASEILTVSPVPIQVAESVLQMEPKESVLDSQIISQEVLSSIITPVHLTETENKIHINDSIEFIESKEKLNLDNITNVKNIDAINKLSKLSIKPKEISLTIEQIQPQFEEEIPTLSSNNFEIPEKLMQKQTEQQRKTSEYCIKEAMKLNKTESEYETNKTLNKTPVRPLRIKELNSSPSTSIKETGQFDSTSQKnullLIKKIKKSTEKSVSIGEHVEAIDDDTEKKITKKIVKKKKLKSEEGLDDNIVDSSSFNKQKKTLKLLKKGIKSSQKTSTDATISEIPTSSTSDLTPVPPKRKVKTIAAKLVIKKSDIE

Target ORF information:

RefSeq Version	XM_012491830.1
Organism	Apis florea(little honeybee)
Definition	Apis florea putative uncharacterized protein DDB_G0282499 (LOC100865528), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012491830.1

ORF Insert Sequence:

ATGGGTGGAT ATGCCTGGGT TACATTAGCT ACCAATGATG CTTATTCCTT GGGAGCTTTA 
GTTTTGGCAC ATTCTTTACA TCGAGTTGGT ACCAAATATG AATTGGCTGT TTTGATTACC 
CCTGGAGTAA CGCAGATAAT GAGGGAAAAA CTTTCAGGAA TCTTTTCTGT AGTAATGGAA 
GTAAATGTAC TTGATTCAAA AGATGAAGCT AATTTGGCAC TTTTAGCTAG ACCAGAATTA 
GGTATTACAT TCACTAAACT TCATTGCTGG AGATTGACTC AATATGAAAA GTGTGTGTTT 
CTTGATGCAG ATACACTTGT TGTCAGAAAT TGTGATGAAC TATTTGAAAG AGAAGAACTA 
TCTGCTGCTC CTGATGTGGG TTGGCCTGAT TGTTTCAATT CTGGTGTATT TGTATACAGA 
CCATCCCAAC AAACTTTTGC TTCTATTACT GCATTTGCTG CTGCTAAAGG TTCATTTGAT 
GGTGGTGACC AAGGATTATT GAATATGTAT TTCAGTGATT GGGCTAGAAA AGATATTTCT 
AAACATTTGC CATTTATTTA TAATATGTGT TCTACTGCTA CATACTCTTA TCTACCTGCA 
TTCAAGCAGT TTGGAGATGA TGTTAGAATA ATTCATTTTA TTGGTATTAC AAAACCATGG 
TTACAGTATT TTGACACATT AACTGGTGTT GTTCAACCAC CAATGGGTTC TACACACTTG 
CAACCATTAT TACAATTATG GTGGAATATA TTTTGTGAAA AAGTGCATTC TCAATTATCA 
CCTGTAATGG CTACCAGCAC ATTGGCACCA ATTTGGCACG AATTTTCTCC TATGTTTTTT 
GAATCCTATG TTCCAAAAAA TCCCATTTGT ACAGACATAC AAGACAAAAC ACAAAATGAC 
ATATATCTAG AACTACCAGA TTTCTCAGAA TTTCAAGATC CATGGGAAAA TTATTATGTA 
CAAAATAATT CAGTTATTTA TAAAACAGAA AATAATGAAA GACAACAGTA TTATTCTGTA 
AACAACTCTT CACCTGTATC TGTATTAAAT GATCAATTTA GTCACATAAA GTCTGTTCAA 
CAGACTCATG AATCATCTCC AAAAAAAAAT AGGGAATTAA TAGGGAATCA TAATCAACAA 
TATAATGAAC ATATTTTTTC TTTGCATACT CAATATCATC AAAATCAAGA ATCTAATCAA 
TTTATCAATA AAATTGAAAA ACGACATTCA TTTTCTCATT CTGATAATAC AACTATTATA 
AATATACAAC CAAATTATTT CCAACTTGAA CACAATATAA AAAAATATAT ACCTGTACAA 
AATCATCATT CGAATGAATA TCAACATGAA TCTGTCAACT ATCAATATAA TTCTCAAAAA 
ACTTGCAATA ATAATAATAA TAATAATAAT AATAATAATA ATAATAATAA TAATAATAAG 
AACAACAACA ATAATAATAA CAATAATAAT AATAATAATA TATTACCAAA AGAAACAAAA 
CAACAATATA TTAATTATGA ACATACTTAT CAACATTCTT CTAATAATAT TAATAAATCA 
TTGTCAGAAG AAGTAAAACA TCTACATAAT AAACTTTATA GAGATCATCA TGATCATTAC 
TATCAAATGC TCGATAATAT AAATAATAAA CATTTTCAAC ATCATGAACA AAAAAATGAA 
ACTTGCAATT CTCTAGATGT CAACATTATT CATGATCAAC ATTATATTAT GCTAAATAAT 
ATAACTTATC AGAATAATGT TTTAATTAAG CAAAATATTG CACAAAATGA AAATATGAAC 
GTTACAGACA AACGAACATT AATATCTACT AATCCTTATC CTGATTCCAA ACCTTGTATA 
GATTTCCACA ATGTAGCAAC GCAATCTGAT CCACATATAA TGGATGATCT AAATAATTCA 
AATGCTGGTC TTGCTGGTGC CTTGGCTCAA ATGACATTAG GTGAACCCAG AAGCTCTGAA 
CAAATTGCAT TAGAAGAACA TATGCGGAAA CAAAGTTGGG AACAAGGTCA AATAGACTAT 
ATGGGTCGAG ATAGTTTTGA TAATATTTGG AAAAAAATTT GTGAAACTCT TGCTCTTGCA 
CCTCCACGAT TACCATCTCC TCCTAAAGAA ATACAAAAGT CTGTCAAAAC TACAATTGAT 
GAAACTGATG AATTTATTAA ATCTGCTGAA ATTACTCAAT TTATCAAGTC TACAGATGAA 
GTGGATAAAA GAACCGCTCA AACAAACAGT CATACTGAAG AATCTCTAGT TAATAATGTT 
CCTACCACAA ATGTACCTAA ATTATTGGAA AATCAAAAGA GTGAAGATGA TATTTCAAGA 
AAATCACGAA ATAATACATT TATAGATTTT CCTGCAACAA ACCTTTCTGT ACAAACATCT 
CATGAAGAAA TAACAGAACT TAATACAACT GAATTATTAA ATAAAATTTC ACAACAGACT 
AGTGTACCTA TAGTATCGTC TGATTTAAAT CAATCTTGTT CAATTGGAGA ACAGAAAATA 
ATTATTTCAA CAAAAGAATT AAAAAACAAA TTACAGAATA AAGATCTTAA AATTACCACA 
GATATTCATC AAATGAATCA ACCAGATATT AGAGATGAAA AATATGCAAA AGAATTATTA 
ACTGCATCTG AAATTTTAAC AGTTAGCCCT GTTCCGATTC AAGTTGCAGA ATCTGTATTA 
CAAATGGAAC CAAAAGAATC TGTGTTAGAT TCTCAAATAA TATCGCAAGA AGTTCTTTCT 
TCTATAATTA CACCAGTTCA TTTAACAGAA ACAGAAAATA AAATACATAT TAATGATTCA 
ATAGAGTTCA TAGAAAGTAA AGAGAAATTA AATTTAGATA ATATCACAAA TGTAAAAAAT 
ATTGATGCTA TCAATAAATT AAGCAAATTG TCAATAAAAC CAAAAGAAAT CTCATTGACA 
ATAGAACAAA TTCAACCTCA ATTTGAAGAA GAAATACCTA CATTATCTTC AAATAACTTT 
GAAATACCAG AGAAATTAAT GCAAAAACAA ACAGAACAAC AAAGAAAAAC AAGTGAATAT 
TGTATAAAAG AAGCAATGAA ATTAAATAAA ACAGAATCAG AATATGAAAC AAATAAAACA 
TTAAACAAAA CTCCAGTTAG ACCTTTAAGA ATAAAAGAAT TAAATTCTTC ACCTTCAACA 
AGTATTAAAG AAACTGGACA ATTTGATTCA ACAAGTCAAA AGNAATTAAT AAAAAAGATA 
AAAAAATCTA CAGAAAAATC AGTATCAATA GGAGAACATG TAGAAGCAAT TGATGATGAT 
ACTGAAAAAA AAATAACAAA GAAGATAGTA AAAAAGAAAA AATTAAAATC AGAAGAAGGA 
CTAGATGATA ATATAGTAGA CAGCAGTTCT TTTAATAAAC AAAAAAAGAC TTTAAAGTTG 
TTAAAAAAAG GTATAAAATC ATCACAAAAA ACTTCTACTG ATGCTACTAT TTCTGAAATT 
CCAACATCTA GCACTTCTGA TTTAACTCCA GTTCCACCTA AAAGAAAAGT GAAAACTATA 
GCTGCAAAAT TGGTTATTAA AAAATCAGAT ATAGAATAG