AgaP_AGAP004707 cDNA ORF clone, Anopheles gambiae str. PEST

Gene
Clones
gRNAs

Summary

Gene Symbol	AgaP_AGAP004707
Entrez Gene ID	1278521
Full Name	AGAP004707-PA
Gene Type	protein-coding
Organism	Anopheles gambiae str. PEST

mRNA and Protein(s)

mRNA	Protein	Name
XM_318122.4	XP_318122.4	AGAP004707-PA
XM_001689051.1	XP_001689103.1	AGAP004707-PB
XM_554647.3	XP_554647.3	AGAP004707-PC

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCN2A	NP_001035233.1
Gallus gallus (chicken)	SCN2A	XP_004942844.1
	SCN2A	XP_001099916.1
Canis lupus familiaris (dog)	SCN2A	XP_535939.2
Rattus norvegicus (Norway rat)	Scn2a1	NP_036779.1
Drosophila melanogaster (fruit fly)	para	NP_001188624.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004707	XP_554647.3
Xenopus tropicalis (tropical clawed frog)	scn3a	XP_002939317.2
Mus musculus (house mouse)	Scn2a1	NP_001092768.1
Bos taurus (cattle)	SCN2A	NP_001137581.1
Pan troglodytes (chimpanzee)	SCN2A	XP_001153227.1
Danio rerio (zebrafish)	scn1lab	NP_001038360.1

Gene Ontology
Bibliography

Related articles in PubMed

The Anopheles gambiae genome: an update.
Mongin E, Louis C, Holt RA, Birney E, Collins FH
Trends in parasitology20(2)49-52(2004 Feb)

The genome sequence of the malaria mosquito Anopheles gambiae.
Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R, Salzberg SL, Loftus B, Yandell M, Majoros WH, Rusch DB, Lai Z, Kraft CL, Abril JF, Anthouard V, Arensburger P, Atkinson PW, Baden H, de Berardinis V, Baldwin D, Benes V, Biedler J, Blass C, Bolanos R, Boscus D, Barnstead M, Cai S, Center A, Chaturverdi K, Christophides GK, Chrystal MA, Clamp M, Cravchik A, Curwen V, Dana A, Delcher A, Dew I, Evans CA, Flanigan M, Grundschober-Freimoser A, Friedli L, Gu Z, Guan P, Guigo R, Hillenmeyer ME, Hladun SL, Hogan JR, Hong YS, Hoover J, Jaillon O, Ke Z, Kodira C, Kokoza E, Koutsos A, Letunic I, Levitsky A, Liang Y, Lin JJ, Lobo NF, Lopez JR, Malek JA, McIntosh TC, Meister S, Miller J, Mobarry C, Mongin E, Murphy SD, O'Brochta DA, Pfannkoch C, Qi R, Regier MA, Remington K, Shao H, Sharakhova MV, Sitter CD, Shetty J, Smith TJ, Strong R, Sun J, Thomasova D, Ton LQ, Topalis P, Tu Z, Unger MF, Walenz B, Wang A, Wang J, Wang M, Wang X, Woodford KJ, Wortman JR, Wu M, Yao A, Zdobnov EM, Zhang H, Zhao Q, Zhao S, Zhu SC, Zhimulev I, Coluzzi M, della Torre A, Roth CW, Louis C, Kalush F, Mural RJ, Myers EW, Adams MD, Smith HO, Broder S, Gardner MJ, Fraser CM, Birney E, Bork P, Brey PT, Venter JC, Weissenbach J, Kafatos FC, Collins FH, Hoffman SL
Science (New York, N.Y.)298(5591)129-49(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mosquitoes in the insecticide-treated net bioassay homozygous for the knockdown resistance kdr-w allele were knocked down significantly faster than those in the WHO bottle bioassay.
Title: The knockdown resistance mutation and knockdown time in Anopheles gambiae collected from Mali evaluated through a bottle bioassay and a novel insecticide-treated net bioassay.

Overall susceptibility (of An. gambiae and An. funestus) to the insecticides was between 80% and 98%, suggesting that there is some level of resistance, except for cyfluthrin 0.15%. The kdr PCR assay however, did not reveal any kdr mutations.
Title: Insecticide resistance profiles for malaria vectors in the Kassena-Nankana district of Ghana.

In bioassays An. funestus was susceptible to permethrin, deltamethrin and DDT. Anopheles gambiae s.l. remained susceptible or tolerant to deltamethrin and resistant to DDT and permethrin, but only when kdr allele carriers reached 90% of the population.
Title: A significant increase in kdr in Anopheles gambiae is associated with an intensive vector control intervention in Burundi highlands.

Knockdown resistance (kdr) is closely associated with DDT and pyrethroid resistance in the major malaria vector Anopheles gambiae and is considered to be causative of the resistance phenotype.
Title: kdr: can a single mutation produce an entire insecticide resistance phenotype?

The frequency of the knockdown resistance (kdr) gene among the M and S subpopulations of An. gambiae in Bioko, Equatorial Guinea, is reported.
Title: An unusual distribution of the kdr gene among populations of Anopheles gambiae on the island of Bioko, Equatorial Guinea.

The following AgaP_AGAP004707 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AgaP_AGAP004707 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAh06073	XM_318122.4 Latest version!	Anopheles gambiae str. PEST AGAP004707-RA (AgaP_AGAP004707), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAh06074	XM_554647.3 Latest version!	Anopheles gambiae str. PEST AGAP004707-RC (AgaP_AGAP004707), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAh06075	XM_001689051.1 Latest version!	Anopheles gambiae str. PEST AGAP004707-RB (AgaP_AGAP004707), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AgaP_AGAP004707 ( XM_318122.4 ) cDNA ORF clone, Anopheles gambiae str. PEST -> XP_318122.4 Anopheles gambiae str. PEST AGAP004707-RA (AgaP_AGAP004707), partial mRNA.

Price & Availability↑

CloneID	OAh06073
Clone ID Related Accession (Same CDS sequence)	XM_318122.4
Accession Version	XM_318122.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-25
Organism	Anopheles gambiae str. PEST
Product	AGAP004707-PA
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078265). On Oct 15, 2007 this sequence version replaced XM_318122.3. COMPLETENESS: incomplete on both ends.

ATGACCGAAG ACTCCGATTC GATATCTGAG GAAGAACGTA GTTTGTTCCG TCCTTTCACT 
CGTGAATCAT TACAAGCTAT CGAAGCACGC ATTGCAGATG AAGAAGCCAA ACAGCGAGAA 
TTGGAAAGAA AACGAGCTGA GGGGGAGGAT GAGGATGAAG GTCCCCAACC GGACCCTACT 
CTTGAACAGG GTGTACCAGT CCCAGTTCGA ATGCAAGGCA GCTTCCCCCC GGAGTTGGCC 
TCCACGCCTC TCGAGGATAT TGACAGTTTC TATTCAAATC AAAGGACATT CGTAGTGATT 
AGTAAAGGAA AAGATATATT TCGTTTCTCC GCAACTAACG CATTATATGT ACTTGATCCG 
TTTAACCCCA TACGCCGCGT AGCTATTTAT ATTTTAGTAC ATCCACTGTT TTCACTTTTT 
ATAATAACGA CCATTCTTGT TAATTGTATA TTGATGATTA TGCCTACCAC GCCGACAGTC 
GAATCTACCG AGGTGATATT CACCGGCATC TACACGTTCG AATCAGCTGT AAAAGTGATG 
GCGCGAGGTT TCATATTACA ACCGTTTACT TATCTTAGAG ATGCATGGAA TTGGTTGGAC 
TTCGTAGTAA TAGCATTAGC ATATGTAACT ATGGGTATAG ATTTGGGTAA TCTTGCTGCG 
TTGAGAACAT TCAGGGTATT ACGAGCTCTT AAAACGGTAG CCATCGTTCC AGGCTTAAAA 
ACCATCGTCG GAGCCGTTAT AGAATCCGTA AAGAATCTCA GAGATGTGAT AATTTTAACA 
ATGTTTTCGT TATCCGTGTT TGCTTTGATG GGTCTACAAA TCTACATGGG AGTACTAACA 
CAAAAGTGCA TAAAAGAGTT CCCATTGGAT GGTTCCTGGG GTAATCTAAC CGACGAAAGC 
TGGGAGCTGT TCAACAGCAA TGACACAAAT TGGTTCTATT CCGAGAGTGG CGACATTCCT 
CTTTGTGGAA ACTCATCTGG AGCTGGACAA TGTGATGAAG GCTACATTTG TTTACAAGGC 
TATGGCAAAA ATCCAAATTA CGGGTATACA AGTTTTGATA CATTCGGATG GGCATTCTTG 
TCTGCCTTTC GTCTAATGAC TCAAGATTAT TGGGAGAATT TATATCAACT GGTGTTACGA 
TCAGCTGGAC CGTGGCACAT GCTCTTCTTC ATTGTGATTA TCTTCTTGGG TTCGTTTTAC 
CTTGTAAATT TAATCTTGGC CATTGTCGCC ATGTCGTACG ACGAACTCCA GAAGAAGGCC 
GAAGAGGAAG AGGCCGCCGA GGAAGAAGCA CTTCGGGAAG CAGAAGAAGC TGCGGCAGCA 
AAGGCAGCTA AACTGGAAGC ACAACAAGCG GCCGCAGCAG CAGCGGCGAA CCCAGAAATC 
GCTAAAAGCC CTTCGGATTT CTCCTGTCAC AGCTATGAGT TGTTTGTCGG ACAGGAGAAA 
GGCAACGACG ATAACAATAA GGAGAAAATG TCCATTAGAA GCGAAGGATT GGAGTCGGTG 
AGCGAAATCA CAAGAACAAC CGCACCAACA GCTACTGCAG CTGGCACTGC AAAAGCCCGT 
AAAGTGAGCG CGGCTTCACT TTCATTACCC GGTTCACCAT TTAATCTTCG TAGAGGATCT 
AGAGGATCAC ACCAGTTCAC GATACGTAAC GGTAGAGGAC GTTTCGTTGG TGTACCTGGT 
AGCGATAGAA AACCACTGGT ACTATCAACA TATCTCGATG CACAAGAACA CCTGCCATAC 
GCTGATGATT CCAACGCGGT AACGCCGATG TCGGAAGAAA ATGGTGCAAT CATCGTTCCA 
GTATACTATG CTAATTTAGG TTCAAGGCAC TCGTCGTATA CTTCGCATCA GTCGCGTATT 
TCGTACACAT CTCACGGTGA CCTGCTCGGG GGCATGACAA AAGAGAGCCG TCTGCGAAAT 
CGATCAGCCC GTAACACTAA CCATTCAATT GTACCACCTC CGAATGCTAA CAATCTATCC 
TACGCTGAAA CAAACCATAA AGGACAGCGA GATTTCGACT TGACACAGGA CTGTACAGAC 
GATGCCGGAA AAATAAAACA TAACGACAAT CCTTTCATAG AACCTGCTCA AACTCAAACT 
GTGGTAGATA TGAAAGACGT GATGGTGTTA AATGACATTA TTGAGCAAGC TGCTGGTCGG 
CACAGCAGGG CAAGTGATCA CGGAGTCTCT GTTTATTACT TCCCCACAGA GGACGACGAC 
GAAGATGGCC CAACGTTTAA GGACAAAGCC CTCGAGTTTC TGATGAAGAT GATCGACATT 
TTCTGTGTGT GGGACTGTTG TTGGGTTTGG CTTAAATTTC AGGAATGGGT TGCTTTTATT 
GTGTTTGACC CATTTGTAGA GCTATTCATT ACGCTCTGCA TTGTGGTAAA TACACTGTTT 
ATGGCTTTAG ATCATCACGA TATGGATCCA GATATGGAAA AGGCACTGAA AAGTGGCAAC 
TACTTCTTCA CAGCTACATT TGCCATCGAA GCTACAATGA AGCTCATAGC AATGAGCCCC 
AAATATTACT TTCAAGAGGG TTGGAATATC TTCGATTTTA TTATCGTAGC ACTGTCTCTG 
CTAGAATTGG GACTTGAGGG TGTTCAAGGA TTGTCAGTAT TACGATCGTT CCGTTTGCTA 
AGAGTTTTCA AGCTGGCAAA ATCATGGCCT ACATTGAATC TTCTAATTTC CATCATGGGA 
CGTACAGTTG GTGCCCTTGG TAATTTAACC TTCGTCTTAT GCATCATCAT TTTCATCTTC 
GCCGTGATGG GGATGCAACT TTTTGGCAAA AACTACACAG ATAATGTGGA TAGATTCCCC 
GACCATGATC TGCCAAGATG GAATTTTACA GATTTCATGC ATTCCTTCAT GATTGTGTTC 
CGTGTGCTAT GCGGAGAATG GATTGAATCA ATGTGGGATT GTATGCTTGT CGGTGATGTA 
TCCTGCATAC CATTTTTCTT GGCCACTGTA GTGATAGGAA ATTTAGTCGT GCTTAACCTT 
TTCTTAGCCT TGCTTTTGTC AAATTTTGGT TCATCATCCT TGTCTGCACC AACGGCAGAT 
AATGAGACCA ACAAGATTGC AGAAGCGTTC AACAGAATAT CACGCTTTTC TAACTGGATT 
AAAATGAATT TAGCAAACGC TCTCAAGTTT GTAAAAAATA AATTAACAAG CCAAATAGCA 
TCCGTTCAAC CGACAGGCAA AGGCGTATGT CCATGTATAT CTTCAGAGCA TGGTGAAAAT 
GAACTGGAAC TTACTCCAGA CGATATTTTG GCGGATGGAC TATTGAAGAA AGGAATCAAA 
GAGCACAACC AACTGGAAGT AGCGATTGGC GATGGCATGG AATTCACCAT TCATGGTGAT 
CTGAAGAACA AAGCAAAAAA GAATAAACAA ATCATGAACA ACTCTAAGGT GATAGGCAAT 
TCTATTAGTA ATCATCAAGA TAATAAATTA GATCATGAAC TGAATCATAG AGGCGTGTCC 
TTACAGGACG ATGATACTGC TAGTATCAAA TCTTATGGCA GTCACAAGAA TCGCCCATTT 
AAGGATGAAA GCCACAAAGG CAGCGCCGAA ACGATGGAGG GTGAAGAAAA ACGTGATGCC 
AGCAAGGAGG ATCTAGGAAT TGACGAAGAA CTCGACGACG AAGGCGAGGG AGATGAAGGT 
CCTCTGGATG GAGAGCTGAT TATTCATGCA GAAGAAGACG AAGTGATTGA GGATTCACCG 
GCGGATTGCT GCCCGGACAA CTGCTACAAA AAATTTCCTG TTCTTGCTGG GGATGATGAC 
GCGCCGTTCT GGCAGGGTTG GGGAAATTTA CGTCTCAAAA CGTTTCAGCT AATAGAAAAT 
AAGTATTTTG AGACAGCTGT AATTACAATG ATTCTGCTTA GTAGCTTAGC TTTGGCCCTC 
GAAGATGTGC ATCTTCCACA GCGCCCAATC CTTCAAGATA TTCTTTATTA CATGGATCGA 
ATTTTCACAG TGATCTTTTT TTTAGAGATG TTAATCAAAT GGTTAGCTTT AGGTTTTAAA 
GTATATTTTA CAAATGCTTG GTGTTGGCTT GATTTCATTA TCGTGATGGT ATCTTTGATA 
AACTTCGTTG CTTCACTTTG TGGAGCTGGT GGTATTCAAG CATTCAAAAC CATGCGAACT 
CTTAGAGCCC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGAATGAG GGTCGTCGTG 
AATGCGTTGG TTCAAGCTAT ACCGTCCATC TTCAACGTGC TGCTGGTTTG TTTGATATTC 
TGGCTAATAT TTGCAATCAT GGGGGTGCAA TTATTTGCTG GCAAATACTT CAAGTGTGTG 
GATAAAAATA AAACTACATT ACCTCACGAA ATTATACCGG ATGTAAATGC TTGCAAAGCC 
GAAAACTATT CATGGGAAAA TTCACCAATG AACTTCGATC ATGTAGGTAA AGCATATTTG 
TGTCTGTTTC AAGTAGCCAC ATTCAAAGGA TGGATACAAA TAATGAACGA TGCTATTGAT 
TCTAGAGACG TAAGTTTTGT CGGTAAACAG CCTATACGGG AAACGAATAT CTACATGTAT 
CTGTACTTTG TGTTCTTTAT TATCTTTGGG TCATTCTTCA CGTTGAATCT ATTCATTGGT 
GTTATAATTG ACAACTTCAA TGAACAGAAA AAGAAAGCTG GTGGATCGCT AGAAATGTTC 
ATGACAGAGG ATCAGAAAAA GTACTATAAT GCAATGAAAA AAATGGGTTC GAAGAAACCT 
CTAAAGGCAA TTCCTCGTCC AAGGTGGCGG CCTCAAGCAA TAGTTTTTGA AATAGTGACG 
AACAAAAAGT TTGACATGAT TATCATGTTG TTCATCGGAT TCAATATGTT AACTATGACA 
CTGGACCACT ACAAACAATC AGAAACTTTT AGTGCTGTTT TGGATTACTT GAATATGATA 
TTCATCTGCA TATTCAGCAG CGAATGTTTA ATGAAGATTT TTGCACTTCG TTATCATTAC 
TTTATCGAGC CATGGAATTT GTTTGATTTT GTTGTCGTCA TTCTTTCGAT TTTGGGCCTT 
GTTCTAAGTG ATATCATTGA AAAATATTTT GTATCTCCCA CACTTCTACG AGTCGTGCGA 
GTGGCAAAAG TGGGCCGAGT ATTGCGTTTG GTTAAAGGAG CCAAGGGTAT CCGAACGTTG 
CTGTTTGCAT TAGCAATGTC GCTACCTGCA CTATTTAACA TCTGCTTGTT ACTCTTTTTG 
GTGATGTTTA TATTTGCCAT TTTTGGAATG TCATTTTTCA TGCACGTCAA AGATAAGAGT 
GGCTTAGATG ACGTGTACAA TTTTAAAACG TTTGGCCAGA GCATGATTTT ACTATTTCAG 
ATGTCAACCT CGGCTGGGTG GGATGGTGTT TTAGATGGTA TTATCAATGA AGAAGACTGT 
CTTCCACCAG ACAATGATAA GGGCTATCCG GGAAATTGTG GTTCATCAAC AATTGGCATA 
ACGTACTTAT TGGCGTATCT TGTAATAAGT TTCCTTATCG TTATTAACAT GTACATTGCT 
GTTATCCTCG AAAACTACTC GCAAGCTACG GAAGATGTTC AAGAAGGCTT AACTGATGAC 
GATTATGATA TGTACTACGA AATATGGCAG CAATTCGATC CTGACGGTAC ACAATACGTT 
CGATATGATC AGCTATCAGA CTTTTTGGAT GTGCTGGAAC CGCCTCTACA GATTCATAAA 
CCAAATCGTT ATAAGATTAT TTCGATGGAT ATTCCGATAT GCCGCGGAGA TATGATGTTC 
TGTGTCGATA TTCTAGATGC ACTAACGAAA GATTTTTTTG CTAGAAAAGG AAATCCTATA 
GAAGAAACAG CCGAATTAGG TGAAGTTCAA CAACGCCCAG ACGAAGTTGG TTACGAACCA 
GTATCATCAA CACTTTGGAG GCAGCGTGAA GAGTACTGTG CTCGACTGAT ACAGCATGCG 
TGGAAACGCT ATAAACAGCG TCACGGAGGC GGAACAGACG CTTCAGGAGA TGATCTTGAA 
ATAGATGCCT GTGATAACGG TTGTGGTGGT GGTAATGGCA ATGAAAATGA TGATAGTGGA 
GATGGTGCAA CAGGTAGTGG TGACAACGGA AGTCAGCATG GTGGTGGCAG CATAAGTGGC 
GGAGGAGGAA CTCCTGGTGG TGGTAAAAGT AAAGGAATTA TTGGCAGTAC TCAGGCTAAC 
ATAGGCATAG TGGATAGTAA TATATCACCA AAGGAATCAC CGGATAGCAT CGGCGATCCC 
CAAGGTCGTC AGACGGCCGT TCTTGTGGAG AGCGACGGAT TTGTGACGAA AAACGGTCAC 
CGTGTCGTCA TACACTCTCG ATCTCCCAGC ATAACATCGC GAACGGCAGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_318122.4
CDS	1..6357
Translation	MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAASLSLPGSPFNLRRGSRGSHQFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKVIGNSISNHQDNKLDHELNHRGVSLQDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVSFVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	XM_318122.4
Organism	Anopheles gambiae str. PEST
Definition	Anopheles gambiae str. PEST AGAP004707-RA (AgaP_AGAP004707), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_318122.4

ORF Insert Sequence:

ATGACCGAAG ACTCCGATTC GATATCTGAG GAAGAACGTA GTTTGTTCCG TCCTTTCACT 
CGTGAATCAT TACAAGCTAT CGAAGCACGC ATTGCAGATG AAGAAGCCAA ACAGCGAGAA 
TTGGAAAGAA AACGAGCTGA GGGGGAGGAT GAGGATGAAG GTCCCCAACC GGACCCTACT 
CTTGAACAGG GTGTACCAGT CCCAGTTCGA ATGCAAGGCA GCTTCCCCCC GGAGTTGGCC 
TCCACGCCTC TCGAGGATAT TGACAGTTTC TATTCAAATC AAAGGACATT CGTAGTGATT 
AGTAAAGGAA AAGATATATT TCGTTTCTCC GCAACTAACG CATTATATGT ACTTGATCCG 
TTTAACCCCA TACGCCGCGT AGCTATTTAT ATTTTAGTAC ATCCACTGTT TTCACTTTTT 
ATAATAACGA CCATTCTTGT TAATTGTATA TTGATGATTA TGCCTACCAC GCCGACAGTC 
GAATCTACCG AGGTGATATT CACCGGCATC TACACGTTCG AATCAGCTGT AAAAGTGATG 
GCGCGAGGTT TCATATTACA ACCGTTTACT TATCTTAGAG ATGCATGGAA TTGGTTGGAC 
TTCGTAGTAA TAGCATTAGC ATATGTAACT ATGGGTATAG ATTTGGGTAA TCTTGCTGCG 
TTGAGAACAT TCAGGGTATT ACGAGCTCTT AAAACGGTAG CCATCGTTCC AGGCTTAAAA 
ACCATCGTCG GAGCCGTTAT AGAATCCGTA AAGAATCTCA GAGATGTGAT AATTTTAACA 
ATGTTTTCGT TATCCGTGTT TGCTTTGATG GGTCTACAAA TCTACATGGG AGTACTAACA 
CAAAAGTGCA TAAAAGAGTT CCCATTGGAT GGTTCCTGGG GTAATCTAAC CGACGAAAGC 
TGGGAGCTGT TCAACAGCAA TGACACAAAT TGGTTCTATT CCGAGAGTGG CGACATTCCT 
CTTTGTGGAA ACTCATCTGG AGCTGGACAA TGTGATGAAG GCTACATTTG TTTACAAGGC 
TATGGCAAAA ATCCAAATTA CGGGTATACA AGTTTTGATA CATTCGGATG GGCATTCTTG 
TCTGCCTTTC GTCTAATGAC TCAAGATTAT TGGGAGAATT TATATCAACT GGTGTTACGA 
TCAGCTGGAC CGTGGCACAT GCTCTTCTTC ATTGTGATTA TCTTCTTGGG TTCGTTTTAC 
CTTGTAAATT TAATCTTGGC CATTGTCGCC ATGTCGTACG ACGAACTCCA GAAGAAGGCC 
GAAGAGGAAG AGGCCGCCGA GGAAGAAGCA CTTCGGGAAG CAGAAGAAGC TGCGGCAGCA 
AAGGCAGCTA AACTGGAAGC ACAACAAGCG GCCGCAGCAG CAGCGGCGAA CCCAGAAATC 
GCTAAAAGCC CTTCGGATTT CTCCTGTCAC AGCTATGAGT TGTTTGTCGG ACAGGAGAAA 
GGCAACGACG ATAACAATAA GGAGAAAATG TCCATTAGAA GCGAAGGATT GGAGTCGGTG 
AGCGAAATCA CAAGAACAAC CGCACCAACA GCTACTGCAG CTGGCACTGC AAAAGCCCGT 
AAAGTGAGCG CGGCTTCACT TTCATTACCC GGTTCACCAT TTAATCTTCG TAGAGGATCT 
AGAGGATCAC ACCAGTTCAC GATACGTAAC GGTAGAGGAC GTTTCGTTGG TGTACCTGGT 
AGCGATAGAA AACCACTGGT ACTATCAACA TATCTCGATG CACAAGAACA CCTGCCATAC 
GCTGATGATT CCAACGCGGT AACGCCGATG TCGGAAGAAA ATGGTGCAAT CATCGTTCCA 
GTATACTATG CTAATTTAGG TTCAAGGCAC TCGTCGTATA CTTCGCATCA GTCGCGTATT 
TCGTACACAT CTCACGGTGA CCTGCTCGGG GGCATGACAA AAGAGAGCCG TCTGCGAAAT 
CGATCAGCCC GTAACACTAA CCATTCAATT GTACCACCTC CGAATGCTAA CAATCTATCC 
TACGCTGAAA CAAACCATAA AGGACAGCGA GATTTCGACT TGACACAGGA CTGTACAGAC 
GATGCCGGAA AAATAAAACA TAACGACAAT CCTTTCATAG AACCTGCTCA AACTCAAACT 
GTGGTAGATA TGAAAGACGT GATGGTGTTA AATGACATTA TTGAGCAAGC TGCTGGTCGG 
CACAGCAGGG CAAGTGATCA CGGAGTCTCT GTTTATTACT TCCCCACAGA GGACGACGAC 
GAAGATGGCC CAACGTTTAA GGACAAAGCC CTCGAGTTTC TGATGAAGAT GATCGACATT 
TTCTGTGTGT GGGACTGTTG TTGGGTTTGG CTTAAATTTC AGGAATGGGT TGCTTTTATT 
GTGTTTGACC CATTTGTAGA GCTATTCATT ACGCTCTGCA TTGTGGTAAA TACACTGTTT 
ATGGCTTTAG ATCATCACGA TATGGATCCA GATATGGAAA AGGCACTGAA AAGTGGCAAC 
TACTTCTTCA CAGCTACATT TGCCATCGAA GCTACAATGA AGCTCATAGC AATGAGCCCC 
AAATATTACT TTCAAGAGGG TTGGAATATC TTCGATTTTA TTATCGTAGC ACTGTCTCTG 
CTAGAATTGG GACTTGAGGG TGTTCAAGGA TTGTCAGTAT TACGATCGTT CCGTTTGCTA 
AGAGTTTTCA AGCTGGCAAA ATCATGGCCT ACATTGAATC TTCTAATTTC CATCATGGGA 
CGTACAGTTG GTGCCCTTGG TAATTTAACC TTCGTCTTAT GCATCATCAT TTTCATCTTC 
GCCGTGATGG GGATGCAACT TTTTGGCAAA AACTACACAG ATAATGTGGA TAGATTCCCC 
GACCATGATC TGCCAAGATG GAATTTTACA GATTTCATGC ATTCCTTCAT GATTGTGTTC 
CGTGTGCTAT GCGGAGAATG GATTGAATCA ATGTGGGATT GTATGCTTGT CGGTGATGTA 
TCCTGCATAC CATTTTTCTT GGCCACTGTA GTGATAGGAA ATTTAGTCGT GCTTAACCTT 
TTCTTAGCCT TGCTTTTGTC AAATTTTGGT TCATCATCCT TGTCTGCACC AACGGCAGAT 
AATGAGACCA ACAAGATTGC AGAAGCGTTC AACAGAATAT CACGCTTTTC TAACTGGATT 
AAAATGAATT TAGCAAACGC TCTCAAGTTT GTAAAAAATA AATTAACAAG CCAAATAGCA 
TCCGTTCAAC CGACAGGCAA AGGCGTATGT CCATGTATAT CTTCAGAGCA TGGTGAAAAT 
GAACTGGAAC TTACTCCAGA CGATATTTTG GCGGATGGAC TATTGAAGAA AGGAATCAAA 
GAGCACAACC AACTGGAAGT AGCGATTGGC GATGGCATGG AATTCACCAT TCATGGTGAT 
CTGAAGAACA AAGCAAAAAA GAATAAACAA ATCATGAACA ACTCTAAGGT GATAGGCAAT 
TCTATTAGTA ATCATCAAGA TAATAAATTA GATCATGAAC TGAATCATAG AGGCGTGTCC 
TTACAGGACG ATGATACTGC TAGTATCAAA TCTTATGGCA GTCACAAGAA TCGCCCATTT 
AAGGATGAAA GCCACAAAGG CAGCGCCGAA ACGATGGAGG GTGAAGAAAA ACGTGATGCC 
AGCAAGGAGG ATCTAGGAAT TGACGAAGAA CTCGACGACG AAGGCGAGGG AGATGAAGGT 
CCTCTGGATG GAGAGCTGAT TATTCATGCA GAAGAAGACG AAGTGATTGA GGATTCACCG 
GCGGATTGCT GCCCGGACAA CTGCTACAAA AAATTTCCTG TTCTTGCTGG GGATGATGAC 
GCGCCGTTCT GGCAGGGTTG GGGAAATTTA CGTCTCAAAA CGTTTCAGCT AATAGAAAAT 
AAGTATTTTG AGACAGCTGT AATTACAATG ATTCTGCTTA GTAGCTTAGC TTTGGCCCTC 
GAAGATGTGC ATCTTCCACA GCGCCCAATC CTTCAAGATA TTCTTTATTA CATGGATCGA 
ATTTTCACAG TGATCTTTTT TTTAGAGATG TTAATCAAAT GGTTAGCTTT AGGTTTTAAA 
GTATATTTTA CAAATGCTTG GTGTTGGCTT GATTTCATTA TCGTGATGGT ATCTTTGATA 
AACTTCGTTG CTTCACTTTG TGGAGCTGGT GGTATTCAAG CATTCAAAAC CATGCGAACT 
CTTAGAGCCC TGAGACCACT ACGTGCCATG TCCCGTATGC AGGGAATGAG GGTCGTCGTG 
AATGCGTTGG TTCAAGCTAT ACCGTCCATC TTCAACGTGC TGCTGGTTTG TTTGATATTC 
TGGCTAATAT TTGCAATCAT GGGGGTGCAA TTATTTGCTG GCAAATACTT CAAGTGTGTG 
GATAAAAATA AAACTACATT ACCTCACGAA ATTATACCGG ATGTAAATGC TTGCAAAGCC 
GAAAACTATT CATGGGAAAA TTCACCAATG AACTTCGATC ATGTAGGTAA AGCATATTTG 
TGTCTGTTTC AAGTAGCCAC ATTCAAAGGA TGGATACAAA TAATGAACGA TGCTATTGAT 
TCTAGAGACG TAAGTTTTGT CGGTAAACAG CCTATACGGG AAACGAATAT CTACATGTAT 
CTGTACTTTG TGTTCTTTAT TATCTTTGGG TCATTCTTCA CGTTGAATCT ATTCATTGGT 
GTTATAATTG ACAACTTCAA TGAACAGAAA AAGAAAGCTG GTGGATCGCT AGAAATGTTC 
ATGACAGAGG ATCAGAAAAA GTACTATAAT GCAATGAAAA AAATGGGTTC GAAGAAACCT 
CTAAAGGCAA TTCCTCGTCC AAGGTGGCGG CCTCAAGCAA TAGTTTTTGA AATAGTGACG 
AACAAAAAGT TTGACATGAT TATCATGTTG TTCATCGGAT TCAATATGTT AACTATGACA 
CTGGACCACT ACAAACAATC AGAAACTTTT AGTGCTGTTT TGGATTACTT GAATATGATA 
TTCATCTGCA TATTCAGCAG CGAATGTTTA ATGAAGATTT TTGCACTTCG TTATCATTAC 
TTTATCGAGC CATGGAATTT GTTTGATTTT GTTGTCGTCA TTCTTTCGAT TTTGGGCCTT 
GTTCTAAGTG ATATCATTGA AAAATATTTT GTATCTCCCA CACTTCTACG AGTCGTGCGA 
GTGGCAAAAG TGGGCCGAGT ATTGCGTTTG GTTAAAGGAG CCAAGGGTAT CCGAACGTTG 
CTGTTTGCAT TAGCAATGTC GCTACCTGCA CTATTTAACA TCTGCTTGTT ACTCTTTTTG 
GTGATGTTTA TATTTGCCAT TTTTGGAATG TCATTTTTCA TGCACGTCAA AGATAAGAGT 
GGCTTAGATG ACGTGTACAA TTTTAAAACG TTTGGCCAGA GCATGATTTT ACTATTTCAG 
ATGTCAACCT CGGCTGGGTG GGATGGTGTT TTAGATGGTA TTATCAATGA AGAAGACTGT 
CTTCCACCAG ACAATGATAA GGGCTATCCG GGAAATTGTG GTTCATCAAC AATTGGCATA 
ACGTACTTAT TGGCGTATCT TGTAATAAGT TTCCTTATCG TTATTAACAT GTACATTGCT 
GTTATCCTCG AAAACTACTC GCAAGCTACG GAAGATGTTC AAGAAGGCTT AACTGATGAC 
GATTATGATA TGTACTACGA AATATGGCAG CAATTCGATC CTGACGGTAC ACAATACGTT 
CGATATGATC AGCTATCAGA CTTTTTGGAT GTGCTGGAAC CGCCTCTACA GATTCATAAA 
CCAAATCGTT ATAAGATTAT TTCGATGGAT ATTCCGATAT GCCGCGGAGA TATGATGTTC 
TGTGTCGATA TTCTAGATGC ACTAACGAAA GATTTTTTTG CTAGAAAAGG AAATCCTATA 
GAAGAAACAG CCGAATTAGG TGAAGTTCAA CAACGCCCAG ACGAAGTTGG TTACGAACCA 
GTATCATCAA CACTTTGGAG GCAGCGTGAA GAGTACTGTG CTCGACTGAT ACAGCATGCG 
TGGAAACGCT ATAAACAGCG TCACGGAGGC GGAACAGACG CTTCAGGAGA TGATCTTGAA 
ATAGATGCCT GTGATAACGG TTGTGGTGGT GGTAATGGCA ATGAAAATGA TGATAGTGGA 
GATGGTGCAA CAGGTAGTGG TGACAACGGA AGTCAGCATG GTGGTGGCAG CATAAGTGGC 
GGAGGAGGAA CTCCTGGTGG TGGTAAAAGT AAAGGAATTA TTGGCAGTAC TCAGGCTAAC 
ATAGGCATAG TGGATAGTAA TATATCACCA AAGGAATCAC CGGATAGCAT CGGCGATCCC 
CAAGGTCGTC AGACGGCCGT TCTTGTGGAG AGCGACGGAT TTGTGACGAA AAACGGTCAC 
CGTGTCGTCA TACACTCTCG ATCTCCCAGC ATAACATCGC GAACGGCAGA TGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AgaP_AGAP004707 ( XM_554647.3 ) cDNA ORF clone, Anopheles gambiae str. PEST -> XP_554647.3 Anopheles gambiae str. PEST AGAP004707-RC (AgaP_AGAP004707), partial mRNA.

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CloneID	OAh06074
Clone ID Related Accession (Same CDS sequence)	XM_554647.3
Accession Version	XM_554647.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-25
Organism	Anopheles gambiae str. PEST
Product	AGAP004707-PC
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078265). On Oct 15, 2007 this sequence version replaced XM_554647.2. COMPLETENESS: incomplete on both ends.

ATGACCGAAG ACTCCGATTC GATATCTGAG GAAGAACGTA GTTTGTTCCG TCCTTTCACT 
CGTGAATCAT TACAAGCTAT CGAAGCACGC ATTGCAGATG AAGAAGCCAA ACAGCGAGAA 
TTGGAAAGAA AACGAGCTGA GGGGGAGGAT GAGGATGAAG GTCCCCAACC GGACCCTACT 
CTTGAACAGG GTGTACCAGT CCCAGTTCGA ATGCAAGGCA GCTTCCCCCC GGAGTTGGCC 
TCCACGCCTC TCGAGGATAT TGACAGTTTC TATTCAAATC AAAGGACATT CGTAGTGATT 
AGTAAAGGAA AAGATATATT TCGTTTCTCC GCAACTAACG CATTATATGT ACTTGATCCG 
TTTAACCCCA TACGCCGCGT AGCTATTTAT ATTTTAGTAC ATCCACTGTT TTCACTTTTT 
ATAATAACGA CCATTCTTGT TAATTGTATA TTGATGATTA TGCCTACCAC GCCGACAGTC 
GAATCTACCG AGGTGATATT CACCGGCATC TACACGTTCG AATCAGCTGT AAAAGTGATG 
GCGCGAGGTT TCATATTACA ACCGTTTACT TATCTTAGAG ATGCATGGAA TTGGTTGGAC 
TTCGTAGTAA TAGCATTAGC ATATGTAACT ATGGGTATAG ATTTGGGTAA TCTTGCTGCG 
TTGAGAACAT TCAGGGTATT ACGAGCTCTT AAAACGGTAG CCATCGTTCC AGGCTTAAAA 
ACCATCGTCG GAGCCGTTAT AGAATCCGTA AAGAATCTCA GAGATGTGAT AATTTTAACA 
ATGTTTTCGT TATCCGTGTT TGCTTTGATG GGTCTACAAA TCTACATGGG AGTACTAACA 
CAAAAGTGCA TAAAAGAGTT CCCATTGGAT GGTTCCTGGG GTAATCTAAC CGACGAAAGC 
TGGGAGCTGT TCAACAGCAA TGACACAAAT TGGTTCTATT CCGAGAGTGG CGACATTCCT 
CTTTGTGGAA ACTCATCTGG AGCTGGACAA TGTGATGAAG GCTACATTTG TTTACAAGGC 
TATGGCAAAA ATCCAAATTA CGGGTATACA AGTTTTGATA CATTCGGATG GGCATTCTTG 
TCTGCCTTTC GTCTAATGAC TCAAGATTAT TGGGAGAATT TATATCAACT GGTGTTACGA 
TCAGCTGGAC CGTGGCACAT GCTCTTCTTC ATTGTGATTA TCTTCTTGGG TTCGTTTTAC 
CTTGTAAATT TAATCTTGGC CATTGTCGCC ATGTCGTACG ACGAACTCCA GAAGAAGGCC 
GAAGAGGAAG AGGCCGCCGA GGAAGAAGCA CTTCGGGAAG CAGAAGAAGC TGCGGCAGCA 
AAGGCAGCTA AACTGGAAGC ACAACAAGCG GCCGCAGCAG CAGCGGCGAA CCCAGAAATC 
GCTAAAAGCC CTTCGGATTT CTCCTGTCAC AGCTATGAGT TGTTTGTCGG ACAGGAGAAA 
GGCAACGACG ATAACAATAA GGAGAAAATG TCCATTAGAA GCGAAGGATT GGAGTCGGTG 
AGCGAAATCA CAAGAACAAC CGCACCAACA GCTACTGCAG CTGGCACTGC AAAAGCCCGT 
AAAGTGAGCG CGGCTTCACT TTCATTACCC GGTTCACCAT TTAATCTTCG TAGAGGATCT 
AGAGGATCAC ACCAGTTCAC GATACGTAAC GGTAGAGGAC GTTTCGTTGG TGTACCTGGT 
AGCGATAGAA AACCACTGGT ACTATCAACA TATCTCGATG CACAAGAACA CCTGCCATAC 
GCTGATGATT CCAACGCGGT AACGCCGATG TCGGAAGAAA ATGGTGCAAT CATCGTTCCA 
GTATACTATG CTAATTTAGG TTCAAGGCAC TCGTCGTATA CTTCGCATCA GTCGCGTATT 
TCGTACACAT CTCACGGTGA CCTGCTCGGG GGCATGACAA AAGAGAGCCG TCTGCGAAAT 
CGATCAGCCC GTAACACTAA CCATTCAATT GTACCACCTC CGAATGCTAA CAATCTATCC 
TACGCTGAAA CAAACCATAA AGGACAGCGA GATTTCGACT TGACACAGGA CTGTACAGAC 
GATGCCGGAA AAATAAAACA TAACGACAAT CCTTTCATAG AACCTGCTCA AACTCAAACT 
GTGGTAGATA TGAAAGACGT GATGGTGTTA AATGACATTA TTGAGCAAGC TGCTGGTCGG 
CACAGCAGGG CAAGTGATCA CGGAGTCTCT GTTTATTACT TCCCCACAGA GGACGACGAC 
GAAGATGGCC CAACGTTTAA GGACAAAGCC CTCGAGTTTC TGATGAAGAT GATCGACATT 
TTCTGTGTGT GGGACTGTTG TTGGGTTTGG CTTAAATTTC AGGAATGGGT TGCTTTTATT 
GTGTTTGACC CATTTGTAGA GCTATTCATT ACGCTCTGCA TTGTGGTAAA TACACTGTTT 
ATGGCTTTAG ATCATCACGA TATGGATCCA GATATGGAAA AGGCACTGAA AAGTGGCAAC 
TACTTCTTCA CAGCTACATT TGCCATCGAA GCTACAATGA AGCTCATAGC AATGAGCCCC 
AAATATTACT TTCAAGAGGG TTGGAATATC TTCGATTTTA TTATCGTAGC ACTGTCTCTG 
CTAGAATTGG GACTTGAGGG TGTTCAAGGA TTGTCAGTAT TACGATCGTT CCGTTTGCTA 
AGAGTTTTCA AGCTGGCAAA ATCATGGCCT ACATTGAATC TTCTAATTTC CATCATGGGA 
CGTACAGTTG GTGCCCTTGG TAATTTAACC TTCGTCTTAT GCATCATCAT TTTCATCTTC 
GCCGTGATGG GGATGCAACT TTTTGGCAAA AACTACACAG ATAATGTGGA TAGATTCCCC 
GACCATGATC TGCCAAGATG GAATTTTACA GATTTCATGC ATTCCTTCAT GATTGTGTTC 
CGTGTGCTAT GCGGAGAATG GATTGAATCA ATGTGGGATT GTATGCTTGT CGGTGATGTA 
TCCTGCATAC CATTTTTCTT GGCCACTGTA GTGATAGGAA ATTTAGTCGT GCTTAACCTT 
TTCTTAGCCT TGCTTTTGTC AAATTTTGGT TCATCATCCT TGTCTGCACC AACGGCAGAT 
AATGAGACCA ACAAGATTGC AGAAGCGTTC AACAGAATAT CACGCTTTTC TAACTGGATT 
AAAATGAATT TAGCAAACGC TCTCAAGTTT GTAAAAAATA AATTAACAAG CCAAATAGCA 
TCCGTTCAAC CGACAGGCAA AGGCGTATGT CCATGTATAT CTTCAGAGCA TGGTGAAAAT 
GAACTGGAAC TTACTCCAGA CGATATTTTG GCGGATGGAC TATTGAAGAA AGGAATCAAA 
GAGCACAACC AACTGGAAGT AGCGATTGGC GATGGCATGG AATTCACCAT TCATGGTGAT 
CTGAAGAACA AAGCAAAAAA GAATAAACAA ATCATGAACA ACTCTAAGGA CGATGATACT 
GCTAGTATCA AATCTTATGG CAGTCACAAG AATCGCCCAT TTAAGGATGA AAGCCACAAA 
GGCAGCGCCG AAACGATGGA GGGTGAAGAA AAACGTGATG CCAGCAAGGA GGATCTAGGA 
ATTGACGAAG AACTCGACGA CGAAGGCGAG GGAGATGAAG GTCCTCTGGA TGGAGAGCTG 
ATTATTCATG CAGAAGAAGA CGAAGTGATT GAGGATTCAC CGGCGGATTG CTGCCCGGAC 
AACTGCTACA AAAAATTTCC TGTTCTTGCT GGGGATGATG ACGCGCCGTT CTGGCAGGGT 
TGGGGAAATT TACGTCTCAA AACGTTTCAG CTAATAGAAA ATAAGTATTT TGAGACAGCT 
GTAATTACAA TGATTCTGCT TAGTAGCTTA GCTTTGGCCC TCGAAGATGT GCATCTTCCA 
CAGCGCCCAA TCCTTCAAGA TATTCTTTAT TACATGGATC GAATTTTCAC AGTGATCTTT 
TTTTTAGAGA TGTTAATCAA ATGGTTAGCT TTAGGTTTTA AAGTATATTT TACAAATGCT 
TGGTGTTGGC TTGATTTCAT TATCGTGATG GTATCTTTGA TAAACTTCGT TGCTTCACTT 
TGTGGAGCTG GTGGTATTCA AGCATTCAAA ACCATGCGAA CTCTTAGAGC CCTGAGACCA 
CTACGTGCCA TGTCCCGTAT GCAGGGAATG AGGGTCGTCG TGAATGCGTT GGTTCAAGCT 
ATACCGTCCA TCTTCAACGT GCTGCTGGTT TGTTTGATAT TCTGGCTAAT ATTTGCAATC 
ATGGGGGTGC AATTATTTGC TGGCAAATAC TTCAAGTGTG TGGATAAAAA TAAAACTACA 
TTACCTCACG AAATTATACC GGATGTAAAT GCTTGCAAAG CCGAAAACTA TTCATGGGAA 
AATTCACCAA TGAACTTCGA TCATGTAGGT AAAGCATATT TGTGTCTGTT TCAAGTAGCC 
ACATTCAAAG GATGGATACA AATAATGAAC GATGCTATTG ATTCTAGAGA CGTAAGTTTT 
GTCGGTAAAC AGCCTATACG GGAAACGAAT ATCTACATGT ATCTGTACTT TGTGTTCTTT 
ATTATCTTTG GGTCATTCTT CACGTTGAAT CTATTCATTG GTGTTATAAT TGACAACTTC 
AATGAACAGA AAAAGAAAGC TGGTGGATCG CTAGAAATGT TCATGACAGA GGATCAGAAA 
AAGTACTATA ATGCAATGAA AAAAATGGGT TCGAAGAAAC CTCTAAAGGC AATTCCTCGT 
CCAAGGTGGC GGCCTCAAGC AATAGTTTTT GAAATAGTGA CGAACAAAAA GTTTGACATG 
ATTATCATGT TGTTCATCGG ATTCAATATG TTAACTATGA CACTGGACCA CTACAAACAA 
TCAGAAACTT TTAGTGCTGT TTTGGATTAC TTGAATATGA TATTCATCTG CATATTCAGC 
AGCGAATGTT TAATGAAGAT TTTTGCACTT CGTTATCATT ACTTTATCGA GCCATGGAAT 
TTGTTTGATT TTGTTGTCGT CATTCTTTCG ATTTTGGGCC TTGTTCTAAG TGATATCATT 
GAAAAATATT TTGTATCTCC CACACTTCTA CGAGTCGTGC GAGTGGCAAA AGTGGGCCGA 
GTATTGCGTT TGGTTAAAGG AGCCAAGGGT ATCCGAACGT TGCTGTTTGC ATTAGCAATG 
TCGCTACCTG CACTATTTAA CATCTGCTTG TTACTCTTTT TGGTGATGTT TATATTTGCC 
ATTTTTGGAA TGTCATTTTT CATGCACGTC AAAGATAAGA GTGGCTTAGA TGACGTGTAC 
AATTTTAAAA CGTTTGGCCA GAGCATGATT TTACTATTTC AGATGTCAAC CTCGGCTGGG 
TGGGATGGTG TTTTAGATGG TATTATCAAT GAAGAAGACT GTCTTCCACC AGACAATGAT 
AAGGGCTATC CGGGAAATTG TGGTTCATCA ACAATTGGCA TAACGTACTT ATTGGCGTAT 
CTTGTAATAA GTTTCCTTAT CGTTATTAAC ATGTACATTG CTGTTATCCT CGAAAACTAC 
TCGCAAGCTA CGGAAGATGT TCAAGAAGGC TTAACTGATG ACGATTATGA TATGTACTAC 
GAAATATGGC AGCAATTCGA TCCTGACGGT ACACAATACG TTCGATATGA TCAGCTATCA 
GACTTTTTGG ATGTGCTGGA ACCGCCTCTA CAGATTCATA AACCAAATCG TTATAAGATT 
ATTTCGATGG ATATTCCGAT ATGCCGCGGA GATATGATGT TCTGTGTCGA TATTCTAGAT 
GCACTAACGA AAGATTTTTT TGCTAGAAAA GGAAATCCTA TAGAAGAAAC AGCCGAATTA 
GGTGAAGTTC AACAACGCCC AGACGAAGTT GGTTACGAAC CAGTATCATC AACACTTTGG 
AGGCAGCGTG AAGAGTACTG TGCTCGACTG ATACAGCATG CGTGGAAACG CTATAAACAG 
CGTCACGGAG GCGGAACAGA CGCTTCAGGA GATGATCTTG AAATAGATGC CTGTGATAAC 
GGTTGTGGTG GTGGTAATGG CAATGAAAAT GATGATAGTG GAGATGGTGC AACAGGTAGT 
GGTGACAACG GAAGTCAGCA TGGTGGTGGC AGCATAAGTG GCGGAGGAGG AACTCCTGGT 
GGTGGTAAAA GTAAAGGAAT TATTGGCAGT ACTCAGGCTA ACATAGGCAT AGTGGATAGT 
AATATATCAC CAAAGGAATC ACCGGATAGC ATCGGCGATC CCCAAGGTCG TCAGACGGCC 
GTTCTTGTGG AGAGCGACGG ATTTGTGACG AAAAACGGTC ACCGTGTCGT CATACACTCT 
CGATCTCCCA GCATAACATC GCGAACGGCA GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_554647.3
CDS	1..6279
Translation	MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAASLSLPGSPFNLRRGSRGSHQFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVSFVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	XM_554647.3
Organism	Anopheles gambiae str. PEST
Definition	Anopheles gambiae str. PEST AGAP004707-RC (AgaP_AGAP004707), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_554647.3

ORF Insert Sequence:

ATGACCGAAG ACTCCGATTC GATATCTGAG GAAGAACGTA GTTTGTTCCG TCCTTTCACT 
CGTGAATCAT TACAAGCTAT CGAAGCACGC ATTGCAGATG AAGAAGCCAA ACAGCGAGAA 
TTGGAAAGAA AACGAGCTGA GGGGGAGGAT GAGGATGAAG GTCCCCAACC GGACCCTACT 
CTTGAACAGG GTGTACCAGT CCCAGTTCGA ATGCAAGGCA GCTTCCCCCC GGAGTTGGCC 
TCCACGCCTC TCGAGGATAT TGACAGTTTC TATTCAAATC AAAGGACATT CGTAGTGATT 
AGTAAAGGAA AAGATATATT TCGTTTCTCC GCAACTAACG CATTATATGT ACTTGATCCG 
TTTAACCCCA TACGCCGCGT AGCTATTTAT ATTTTAGTAC ATCCACTGTT TTCACTTTTT 
ATAATAACGA CCATTCTTGT TAATTGTATA TTGATGATTA TGCCTACCAC GCCGACAGTC 
GAATCTACCG AGGTGATATT CACCGGCATC TACACGTTCG AATCAGCTGT AAAAGTGATG 
GCGCGAGGTT TCATATTACA ACCGTTTACT TATCTTAGAG ATGCATGGAA TTGGTTGGAC 
TTCGTAGTAA TAGCATTAGC ATATGTAACT ATGGGTATAG ATTTGGGTAA TCTTGCTGCG 
TTGAGAACAT TCAGGGTATT ACGAGCTCTT AAAACGGTAG CCATCGTTCC AGGCTTAAAA 
ACCATCGTCG GAGCCGTTAT AGAATCCGTA AAGAATCTCA GAGATGTGAT AATTTTAACA 
ATGTTTTCGT TATCCGTGTT TGCTTTGATG GGTCTACAAA TCTACATGGG AGTACTAACA 
CAAAAGTGCA TAAAAGAGTT CCCATTGGAT GGTTCCTGGG GTAATCTAAC CGACGAAAGC 
TGGGAGCTGT TCAACAGCAA TGACACAAAT TGGTTCTATT CCGAGAGTGG CGACATTCCT 
CTTTGTGGAA ACTCATCTGG AGCTGGACAA TGTGATGAAG GCTACATTTG TTTACAAGGC 
TATGGCAAAA ATCCAAATTA CGGGTATACA AGTTTTGATA CATTCGGATG GGCATTCTTG 
TCTGCCTTTC GTCTAATGAC TCAAGATTAT TGGGAGAATT TATATCAACT GGTGTTACGA 
TCAGCTGGAC CGTGGCACAT GCTCTTCTTC ATTGTGATTA TCTTCTTGGG TTCGTTTTAC 
CTTGTAAATT TAATCTTGGC CATTGTCGCC ATGTCGTACG ACGAACTCCA GAAGAAGGCC 
GAAGAGGAAG AGGCCGCCGA GGAAGAAGCA CTTCGGGAAG CAGAAGAAGC TGCGGCAGCA 
AAGGCAGCTA AACTGGAAGC ACAACAAGCG GCCGCAGCAG CAGCGGCGAA CCCAGAAATC 
GCTAAAAGCC CTTCGGATTT CTCCTGTCAC AGCTATGAGT TGTTTGTCGG ACAGGAGAAA 
GGCAACGACG ATAACAATAA GGAGAAAATG TCCATTAGAA GCGAAGGATT GGAGTCGGTG 
AGCGAAATCA CAAGAACAAC CGCACCAACA GCTACTGCAG CTGGCACTGC AAAAGCCCGT 
AAAGTGAGCG CGGCTTCACT TTCATTACCC GGTTCACCAT TTAATCTTCG TAGAGGATCT 
AGAGGATCAC ACCAGTTCAC GATACGTAAC GGTAGAGGAC GTTTCGTTGG TGTACCTGGT 
AGCGATAGAA AACCACTGGT ACTATCAACA TATCTCGATG CACAAGAACA CCTGCCATAC 
GCTGATGATT CCAACGCGGT AACGCCGATG TCGGAAGAAA ATGGTGCAAT CATCGTTCCA 
GTATACTATG CTAATTTAGG TTCAAGGCAC TCGTCGTATA CTTCGCATCA GTCGCGTATT 
TCGTACACAT CTCACGGTGA CCTGCTCGGG GGCATGACAA AAGAGAGCCG TCTGCGAAAT 
CGATCAGCCC GTAACACTAA CCATTCAATT GTACCACCTC CGAATGCTAA CAATCTATCC 
TACGCTGAAA CAAACCATAA AGGACAGCGA GATTTCGACT TGACACAGGA CTGTACAGAC 
GATGCCGGAA AAATAAAACA TAACGACAAT CCTTTCATAG AACCTGCTCA AACTCAAACT 
GTGGTAGATA TGAAAGACGT GATGGTGTTA AATGACATTA TTGAGCAAGC TGCTGGTCGG 
CACAGCAGGG CAAGTGATCA CGGAGTCTCT GTTTATTACT TCCCCACAGA GGACGACGAC 
GAAGATGGCC CAACGTTTAA GGACAAAGCC CTCGAGTTTC TGATGAAGAT GATCGACATT 
TTCTGTGTGT GGGACTGTTG TTGGGTTTGG CTTAAATTTC AGGAATGGGT TGCTTTTATT 
GTGTTTGACC CATTTGTAGA GCTATTCATT ACGCTCTGCA TTGTGGTAAA TACACTGTTT 
ATGGCTTTAG ATCATCACGA TATGGATCCA GATATGGAAA AGGCACTGAA AAGTGGCAAC 
TACTTCTTCA CAGCTACATT TGCCATCGAA GCTACAATGA AGCTCATAGC AATGAGCCCC 
AAATATTACT TTCAAGAGGG TTGGAATATC TTCGATTTTA TTATCGTAGC ACTGTCTCTG 
CTAGAATTGG GACTTGAGGG TGTTCAAGGA TTGTCAGTAT TACGATCGTT CCGTTTGCTA 
AGAGTTTTCA AGCTGGCAAA ATCATGGCCT ACATTGAATC TTCTAATTTC CATCATGGGA 
CGTACAGTTG GTGCCCTTGG TAATTTAACC TTCGTCTTAT GCATCATCAT TTTCATCTTC 
GCCGTGATGG GGATGCAACT TTTTGGCAAA AACTACACAG ATAATGTGGA TAGATTCCCC 
GACCATGATC TGCCAAGATG GAATTTTACA GATTTCATGC ATTCCTTCAT GATTGTGTTC 
CGTGTGCTAT GCGGAGAATG GATTGAATCA ATGTGGGATT GTATGCTTGT CGGTGATGTA 
TCCTGCATAC CATTTTTCTT GGCCACTGTA GTGATAGGAA ATTTAGTCGT GCTTAACCTT 
TTCTTAGCCT TGCTTTTGTC AAATTTTGGT TCATCATCCT TGTCTGCACC AACGGCAGAT 
AATGAGACCA ACAAGATTGC AGAAGCGTTC AACAGAATAT CACGCTTTTC TAACTGGATT 
AAAATGAATT TAGCAAACGC TCTCAAGTTT GTAAAAAATA AATTAACAAG CCAAATAGCA 
TCCGTTCAAC CGACAGGCAA AGGCGTATGT CCATGTATAT CTTCAGAGCA TGGTGAAAAT 
GAACTGGAAC TTACTCCAGA CGATATTTTG GCGGATGGAC TATTGAAGAA AGGAATCAAA 
GAGCACAACC AACTGGAAGT AGCGATTGGC GATGGCATGG AATTCACCAT TCATGGTGAT 
CTGAAGAACA AAGCAAAAAA GAATAAACAA ATCATGAACA ACTCTAAGGA CGATGATACT 
GCTAGTATCA AATCTTATGG CAGTCACAAG AATCGCCCAT TTAAGGATGA AAGCCACAAA 
GGCAGCGCCG AAACGATGGA GGGTGAAGAA AAACGTGATG CCAGCAAGGA GGATCTAGGA 
ATTGACGAAG AACTCGACGA CGAAGGCGAG GGAGATGAAG GTCCTCTGGA TGGAGAGCTG 
ATTATTCATG CAGAAGAAGA CGAAGTGATT GAGGATTCAC CGGCGGATTG CTGCCCGGAC 
AACTGCTACA AAAAATTTCC TGTTCTTGCT GGGGATGATG ACGCGCCGTT CTGGCAGGGT 
TGGGGAAATT TACGTCTCAA AACGTTTCAG CTAATAGAAA ATAAGTATTT TGAGACAGCT 
GTAATTACAA TGATTCTGCT TAGTAGCTTA GCTTTGGCCC TCGAAGATGT GCATCTTCCA 
CAGCGCCCAA TCCTTCAAGA TATTCTTTAT TACATGGATC GAATTTTCAC AGTGATCTTT 
TTTTTAGAGA TGTTAATCAA ATGGTTAGCT TTAGGTTTTA AAGTATATTT TACAAATGCT 
TGGTGTTGGC TTGATTTCAT TATCGTGATG GTATCTTTGA TAAACTTCGT TGCTTCACTT 
TGTGGAGCTG GTGGTATTCA AGCATTCAAA ACCATGCGAA CTCTTAGAGC CCTGAGACCA 
CTACGTGCCA TGTCCCGTAT GCAGGGAATG AGGGTCGTCG TGAATGCGTT GGTTCAAGCT 
ATACCGTCCA TCTTCAACGT GCTGCTGGTT TGTTTGATAT TCTGGCTAAT ATTTGCAATC 
ATGGGGGTGC AATTATTTGC TGGCAAATAC TTCAAGTGTG TGGATAAAAA TAAAACTACA 
TTACCTCACG AAATTATACC GGATGTAAAT GCTTGCAAAG CCGAAAACTA TTCATGGGAA 
AATTCACCAA TGAACTTCGA TCATGTAGGT AAAGCATATT TGTGTCTGTT TCAAGTAGCC 
ACATTCAAAG GATGGATACA AATAATGAAC GATGCTATTG ATTCTAGAGA CGTAAGTTTT 
GTCGGTAAAC AGCCTATACG GGAAACGAAT ATCTACATGT ATCTGTACTT TGTGTTCTTT 
ATTATCTTTG GGTCATTCTT CACGTTGAAT CTATTCATTG GTGTTATAAT TGACAACTTC 
AATGAACAGA AAAAGAAAGC TGGTGGATCG CTAGAAATGT TCATGACAGA GGATCAGAAA 
AAGTACTATA ATGCAATGAA AAAAATGGGT TCGAAGAAAC CTCTAAAGGC AATTCCTCGT 
CCAAGGTGGC GGCCTCAAGC AATAGTTTTT GAAATAGTGA CGAACAAAAA GTTTGACATG 
ATTATCATGT TGTTCATCGG ATTCAATATG TTAACTATGA CACTGGACCA CTACAAACAA 
TCAGAAACTT TTAGTGCTGT TTTGGATTAC TTGAATATGA TATTCATCTG CATATTCAGC 
AGCGAATGTT TAATGAAGAT TTTTGCACTT CGTTATCATT ACTTTATCGA GCCATGGAAT 
TTGTTTGATT TTGTTGTCGT CATTCTTTCG ATTTTGGGCC TTGTTCTAAG TGATATCATT 
GAAAAATATT TTGTATCTCC CACACTTCTA CGAGTCGTGC GAGTGGCAAA AGTGGGCCGA 
GTATTGCGTT TGGTTAAAGG AGCCAAGGGT ATCCGAACGT TGCTGTTTGC ATTAGCAATG 
TCGCTACCTG CACTATTTAA CATCTGCTTG TTACTCTTTT TGGTGATGTT TATATTTGCC 
ATTTTTGGAA TGTCATTTTT CATGCACGTC AAAGATAAGA GTGGCTTAGA TGACGTGTAC 
AATTTTAAAA CGTTTGGCCA GAGCATGATT TTACTATTTC AGATGTCAAC CTCGGCTGGG 
TGGGATGGTG TTTTAGATGG TATTATCAAT GAAGAAGACT GTCTTCCACC AGACAATGAT 
AAGGGCTATC CGGGAAATTG TGGTTCATCA ACAATTGGCA TAACGTACTT ATTGGCGTAT 
CTTGTAATAA GTTTCCTTAT CGTTATTAAC ATGTACATTG CTGTTATCCT CGAAAACTAC 
TCGCAAGCTA CGGAAGATGT TCAAGAAGGC TTAACTGATG ACGATTATGA TATGTACTAC 
GAAATATGGC AGCAATTCGA TCCTGACGGT ACACAATACG TTCGATATGA TCAGCTATCA 
GACTTTTTGG ATGTGCTGGA ACCGCCTCTA CAGATTCATA AACCAAATCG TTATAAGATT 
ATTTCGATGG ATATTCCGAT ATGCCGCGGA GATATGATGT TCTGTGTCGA TATTCTAGAT 
GCACTAACGA AAGATTTTTT TGCTAGAAAA GGAAATCCTA TAGAAGAAAC AGCCGAATTA 
GGTGAAGTTC AACAACGCCC AGACGAAGTT GGTTACGAAC CAGTATCATC AACACTTTGG 
AGGCAGCGTG AAGAGTACTG TGCTCGACTG ATACAGCATG CGTGGAAACG CTATAAACAG 
CGTCACGGAG GCGGAACAGA CGCTTCAGGA GATGATCTTG AAATAGATGC CTGTGATAAC 
GGTTGTGGTG GTGGTAATGG CAATGAAAAT GATGATAGTG GAGATGGTGC AACAGGTAGT 
GGTGACAACG GAAGTCAGCA TGGTGGTGGC AGCATAAGTG GCGGAGGAGG AACTCCTGGT 
GGTGGTAAAA GTAAAGGAAT TATTGGCAGT ACTCAGGCTA ACATAGGCAT AGTGGATAGT 
AATATATCAC CAAAGGAATC ACCGGATAGC ATCGGCGATC CCCAAGGTCG TCAGACGGCC 
GTTCTTGTGG AGAGCGACGG ATTTGTGACG AAAAACGGTC ACCGTGTCGT CATACACTCT 
CGATCTCCCA GCATAACATC GCGAACGGCA GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AgaP_AGAP004707 ( XM_001689051.1 ) cDNA ORF clone, Anopheles gambiae str. PEST -> XP_001689103.1 Anopheles gambiae str. PEST AGAP004707-RB (AgaP_AGAP004707), partial mRNA.

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CloneID	OAh06075
Clone ID Related Accession (Same CDS sequence)	XM_001689051.1
Accession Version	XM_001689051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-25
Organism	Anopheles gambiae str. PEST
Product	AGAP004707-PB
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078265). COMPLETENESS: incomplete on both ends.

ATGACCGAAG ACTCCGATTC GATATCTGAG GAAGAACGTA GTTTGTTCCG TCCTTTCACT 
CGTGAATCAT TACAAGCTAT CGAAGCACGC ATTGCAGATG AAGAAGCCAA ACAGCGAGAA 
TTGGAAAGAA AACGAGCTGA GGGGGAGGAT GAGGATGAAG GTCCCCAACC GGACCCTACT 
CTTGAACAGG GTGTACCAGT CCCAGTTCGA ATGCAAGGCA GCTTCCCCCC GGAGTTGGCC 
TCCACGCCTC TCGAGGATAT TGACAGTTTC TATTCAAATC AAAGGACATT CGTAGTGATT 
AGTAAAGGAA AAGATATATT TCGTTTCTCC GCAACTAACG CATTATATGT ACTTGATCCG 
TTTAACCCCA TACGCCGCGT AGCTATTTAT ATTTTAGTAC ATCCACTGTT TTCACTTTTT 
ATAATAACGA CCATTCTTGT TAATTGTATA TTGATGATTA TGCCTACCAC GCCGACAGTC 
GAATCTACCG AGGTGATATT CACCGGCATC TACACGTTCG AATCAGCTGT AAAAGTGATG 
GCGCGAGGTT TCATATTACA ACCGTTTACT TATCTTAGAG ATGCATGGAA TTGGTTGGAC 
TTCGTAGTAA TAGCATTAGC ATATGTAACT ATGGGTATAG ATTTGGGTAA TCTTGCTGCG 
TTGAGAACAT TCAGGGTATT ACGAGCTCTT AAAACGGTAG CCATCGTTCC AGGCTTAAAA 
ACCATCGTCG GAGCCGTTAT AGAATCCGTA AAGAATCTCA GAGATGTGAT AATTTTAACA 
ATGTTTTCGT TATCCGTGTT TGCTTTGATG GGTCTACAAA TCTACATGGG AGTACTAACA 
CAAAAGTGCA TAAAAGAGTT CCCATTGGAT GGTTCCTGGG GTAATCTAAC CGACGAAAGC 
TGGGAGCTGT TCAACAGCAA TGACACAAAT TGGTTCTATT CCGAGAGTGG CGACATTCCT 
CTTTGTGGAA ACTCATCTGG AGCTGGACAA TGTGATGAAG GCTACATTTG TTTACAAGGC 
TATGGCAAAA ATCCAAATTA CGGGTATACA AGTTTTGATA CATTCGGATG GGCATTCTTG 
TCTGCCTTTC GTCTAATGAC TCAAGATTAT TGGGAGAATT TATATCAACT GGTGTTACGA 
TCAGCTGGAC CGTGGCACAT GCTCTTCTTC ATTGTGATTA TCTTCTTGGG TTCGTTTTAC 
CTTGTAAATT TAATCTTGGC CATTGTCGCC ATGTCGTACG ACGAACTCCA GAAGAAGGCC 
GAAGAGGAAG AGGCCGCCGA GGAAGAAGCA CTTCGGGAAG CAGAAGAAGC TGCGGCAGCA 
AAGGCAGCTA AACTGGAAGC ACAACAAGCG GCCGCAGCAG CAGCGGCGAA CCCAGAAATC 
GCTAAAAGCC CTTCGGATTT CTCCTGTCAC AGCTATGAGT TGTTTGTCGG ACAGGAGAAA 
GGCAACGACG ATAACAATAA GGAGAAAATG TCCATTAGAA GCGAAGGATT GGAGTCGGTG 
AGCGAAATCA CAAGAACAAC CGCACCAACA GCTACTGCAG CTGGCACTGC AAAAGCCCGT 
AAAGTGAGCG CGGCTTCACT TTCATTACCC GGTTCACCAT TTAATCTTCG TAGAGGATCT 
AGAGGATCAC ACCAGTTCAC GATACGTAAC GGTAGAGGAC GTTTCGTTGG TGTACCTGGT 
AGCGATAGAA AACCACTGGT ACTATCAACA TATCTCGATG CACAAGAACA CCTGCCATAC 
GCTGATGATT CCAACGCGGT AACGCCGATG TCGGAAGAAA ATGGTGCAAT CATCGTTCCA 
GTATACTATG CTAATTTAGG TTCAAGGCAC TCGTCGTATA CTTCGCATCA GTCGCGTATT 
TCGTACACAT CTCACGGTGA CCTGCTCGGG GGCATGACAA AAGAGAGCCG TCTGCGAAAT 
CGATCAGCCC GTAACACTAA CCATTCAATT GTACCACCTC CGAATGCTAA CAATCTATCC 
TACGCTGAAA CAAACCATAA AGGACAGCGA GATTTCGACT TGACACAGGA CTGTACAGAC 
GATGCCGGAA AAATAAAACA TAACGACAAT CCTTTCATAG AACCTGCTCA AACTCAAACT 
GTGGTAGATA TGAAAGACGT GATGGTGTTA AATGACATTA TTGAGCAAGC TGCTGGTCGG 
CACAGCAGGG CAAGTGATCA CGGAGTCTCT GTTTATTACT TCCCCACAGA GGACGACGAC 
GAAGATGGCC CAACGTTTAA GGACAAAGCC CTCGAGTTTC TGATGAAGAT GATCGACATT 
TTCTGTGTGT GGGACTGTTG TTGGGTTTGG CTTAAATTTC AGGAATGGGT TGCTTTTATT 
GTGTTTGACC CATTTGTAGA GCTATTCATT ACGCTCTGCA TTGTGGTAAA TACACTGTTT 
ATGGCTTTAG ATCATCACGA TATGGATCCA GATATGGAAA AGGCACTGAA AAGTGGCAAC 
TACTTCTTCA CAGCTACATT TGCCATCGAA GCTACAATGA AGCTCATAGC AATGAGCCCC 
AAATATTACT TTCAAGAGGG TTGGAATATC TTCGATTTTA TTATCGTAGC ACTGTCTCTG 
CTAGAATTGG GACTTGAGGG TGTTCAAGGA TTGTCAGTAT TACGATCGTT CCGTTTGCTT 
CGAGTTTTTA AGCTCGCTAA ATCGTGGCCA ACGCTGAATT TACTCATTTC CATCATGGGC 
AGAACGATGG GTGCGTTAGG TAATCTGACG TTCGTACTCT GCATTATCAT CTTCATCTTT 
GCCGTGATGG GAATGCAGCT GTTCGGAAAG AACTATGTCG ATAATGTGGA TAGATTCCCC 
GACCATGATC TGCCAAGATG GAATTTTACA GATTTCATGC ATTCCTTCAT GATTGTGTTC 
CGTGTGCTAT GCGGAGAATG GATTGAATCA ATGTGGGATT GTATGCTTGT CGGTGATGTA 
TCCTGCATAC CATTTTTCTT GGCCACTGTA GTGATAGGAA ATTTAGTCGT GCTTAACCTT 
TTCTTAGCCT TGCTTTTGTC AAATTTTGGT TCATCATCCT TGTCTGCACC AACGGCAGAT 
AATGAGACCA ACAAGATTGC AGAAGCGTTC AACAGAATAT CACGCTTTTC TAACTGGATT 
AAAATGAATT TAGCAAACGC TCTCAAGTTT GTAAAAAATA AATTAACAAG CCAAATAGCA 
TCCGTTCAAC CGACAGGCAA AGGCGTATGT CCATGTATAT CTTCAGAGCA TGGTGAAAAT 
GAACTGGAAC TTACTCCAGA CGATATTTTG GCGGATGGAC TATTGAAGAA AGGAATCAAA 
GAGCACAACC AACTGGAAGT AGCGATTGGC GATGGCATGG AATTCACCAT TCATGGTGAT 
CTGAAGAACA AAGCAAAAAA GAATAAACAA ATCATGAACA ACTCTAAGGA CGATGATACT 
GCTAGTATCA AATCTTATGG CAGTCACAAG AATCGCCCAT TTAAGGATGA AAGCCACAAA 
GGCAGCGCCG AAACGATGGA GGGTGAAGAA AAACGTGATG CCAGCAAGGA GGATCTAGGA 
ATTGACGAAG AACTCGACGA CGAAGGCGAG GGAGATGAAG GTCCTCTGGA TGGAGAGCTG 
ATTATTCATG CAGAAGAAGA CGAAGTGATT GAGGATTCAC CGGCGGATTG CTGCCCGGAC 
AACTGCTACA AAAAATTTCC TGTTCTTGCT GGGGATGATG ACGCGCCGTT CTGGCAGGGT 
TGGGGAAATT TACGTCTCAA AACGTTTCAG CTAATAGAAA ATAAGTATTT TGAGACAGCT 
GTAATTACAA TGATTCTGCT TAGTAGCTTA GCTTTGGCCC TCGAAGATGT GCATCTTCCA 
CAGCGCCCAA TCCTTCAAGA TATTCTTTAT TACATGGATC GAATTTTCAC AGTGATCTTT 
TTTTTAGAGA TGTTAATCAA ATGGTTAGCT TTAGGTTTTA AAGTATATTT TACAAATGCT 
TGGTGTTGGC TTGATTTCAT TATCGTGATG GTATCTTTGA TAAACTTCGT TGCTTCACTT 
TGTGGAGCTG GTGGTATTCA AGCATTCAAA ACCATGCGAA CTCTTAGAGC CCTGAGACCA 
CTACGTGCCA TGTCCCGTAT GCAGGGAATG AGGGTCGTCG TGAATGCGTT GGTTCAAGCT 
ATACCGTCCA TCTTCAACGT GCTGCTGGTT TGTTTGATAT TCTGGCTAAT ATTTGCAATC 
ATGGGGGTGC AATTATTTGC TGGCAAATAC TTCAAGTGTG TGGATAAAAA TAAAACTACA 
TTACCTCACG AAATTATACC GGATGTAAAT GCTTGCAAAG CCGAAAACTA TTCATGGGAA 
AATTCACCAA TGAACTTCGA TCATGTAGGT AAAGCATATT TGTGTCTGTT TCAAGTAGCC 
ACATTCAAAG GATGGATACA AATAATGAAC GATGCTATTG ATTCTAGAGA CGTAAGTTTT 
GTCGGTAAAC AGCCTATACG GGAAACGAAT ATCTACATGT ATCTGTACTT TGTGTTCTTT 
ATTATCTTTG GGTCATTCTT CACGTTGAAT CTATTCATTG GTGTTATAAT TGACAACTTC 
AATGAACAGA AAAAGAAAGC TGGTGGATCG CTAGAAATGT TCATGACAGA GGATCAGAAA 
AAGTACTATA ATGCAATGAA AAAAATGGGT TCGAAGAAAC CTCTAAAGGC AATTCCTCGT 
CCAAGGTGGC GGCCTCAAGC AATAGTTTTT GAAATAGTGA CGAACAAAAA GTTTGACATG 
ATTATCATGT TGTTCATCGG ATTCAATATG TTAACTATGA CACTGGACCA CTACAAACAA 
TCAGAAACTT TTAGTGCTGT TTTGGATTAC TTGAATATGA TATTCATCTG CATATTCAGC 
AGCGAATGTT TAATGAAGAT TTTTGCACTT CGTTATCATT ACTTTATCGA GCCATGGAAT 
TTGTTTGATT TTGTTGTCGT CATTCTTTCG ATTTTGGGCC TTGTTCTAAG TGATATCATT 
GAAAAATATT TTGTATCTCC CACACTTCTA CGAGTCGTGC GAGTGGCAAA AGTGGGCCGA 
GTATTGCGTT TGGTTAAAGG AGCCAAGGGT ATCCGAACGT TGCTGTTTGC ATTAGCAATG 
TCGCTACCTG CACTATTTAA CATCTGCTTG TTACTCTTTT TGGTGATGTT TATATTTGCC 
ATTTTTGGAA TGTCATTTTT CATGCACGTC AAAGATAAGA GTGGCTTAGA TGACGTGTAC 
AATTTTAAAA CGTTTGGCCA GAGCATGATT TTACTATTTC AGATGTCAAC CTCGGCTGGG 
TGGGATGGTG TTTTAGATGG TATTATCAAT GAAGAAGACT GTCTTCCACC AGACAATGAT 
AAGGGCTATC CGGGAAATTG TGGTTCATCA ACAATTGGCA TAACGTACTT ATTGGCGTAT 
CTTGTAATAA GTTTCCTTAT CGTTATTAAC ATGTACATTG CTGTTATCCT CGAAAACTAC 
TCGCAAGCTA CGGAAGATGT TCAAGAAGGC TTAACTGATG ACGATTATGA TATGTACTAC 
GAAATATGGC AGCAATTCGA TCCTGACGGT ACACAATACG TTCGATATGA TCAGCTATCA 
GACTTTTTGG ATGTGCTGGA ACCGCCTCTA CAGATTCATA AACCAAATCG TTATAAGATT 
ATTTCGATGG ATATTCCGAT ATGCCGCGGA GATATGATGT TCTGTGTCGA TATTCTAGAT 
GCACTAACGA AAGATTTTTT TGCTAGAAAA GGAAATCCTA TAGAAGAAAC AGCCGAATTA 
GGTGAAGTTC AACAACGCCC AGACGAAGTT GGTTACGAAC CAGTATCATC AACACTTTGG 
AGGCAGCGTG AAGAGTACTG TGCTCGACTG ATACAGCATG CGTGGAAACG CTATAAACAG 
CGTCACGGAG GCGGAACAGA CGCTTCAGGA GATGATCTTG AAATAGATGC CTGTGATAAC 
GGTTGTGGTG GTGGTAATGG CAATGAAAAT GATGATAGTG GAGATGGTGC AACAGGTAGT 
GGTGACAACG GAAGTCAGCA TGGTGGTGGC AGCATAAGTG GCGGAGGAGG AACTCCTGGT 
GGTGGTAAAA GTAAAGGAAT TATTGGCAGT ACTCAGGCTA ACATAGGCAT AGTGGATAGT 
AATATATCAC CAAAGGAATC ACCGGATAGC ATCGGCGATC CCCAAGGTCG TCAGACGGCC 
GTTCTTGTGG AGAGCGACGG ATTTGTGACG AAAAACGGTC ACCGTGTCGT CATACACTCT 
CGATCTCCCA GCATAACATC GCGAACGGCA GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001689103.1
CDS	1..6279
Translation	MTEDSDSISEEERSLFRPFTRESLQAIEARIADEEAKQRELERKRAEGEDEDEGPQPDPTLEQGVPVPVRMQGSFPPELASTPLEDIDSFYSNQRTFVVISKGKDIFRFSATNALYVLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILQPFTYLRDAWNWLDFVVIALAYVTMGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKEFPLDGSWGNLTDESWELFNSNDTNWFYSESGDIPLCGNSSGAGQCDEGYICLQGYGKNPNYGYTSFDTFGWAFLSAFRLMTQDYWENLYQLVLRSAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSYDELQKKAEEEEAAEEEALREAEEAAAAKAAKLEAQQAAAAAAANPEIAKSPSDFSCHSYELFVGQEKGNDDNNKEKMSIRSEGLESVSEITRTTAPTATAAGTAKARKVSAASLSLPGSPFNLRRGSRGSHQFTIRNGRGRFVGVPGSDRKPLVLSTYLDAQEHLPYADDSNAVTPMSEENGAIIVPVYYANLGSRHSSYTSHQSRISYTSHGDLLGGMTKESRLRNRSARNTNHSIVPPPNANNLSYAETNHKGQRDFDLTQDCTDDAGKIKHNDNPFIEPAQTQTVVDMKDVMVLNDIIEQAAGRHSRASDHGVSVYYFPTEDDDEDGPTFKDKALEFLMKMIDIFCVWDCCWVWLKFQEWVAFIVFDPFVELFITLCIVVNTLFMALDHHDMDPDMEKALKSGNYFFTATFAIEATMKLIAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYVDNVDRFPDHDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMLVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADNETNKIAEAFNRISRFSNWIKMNLANALKFVKNKLTSQIASVQPTGKGVCPCISSEHGENELELTPDDILADGLLKKGIKEHNQLEVAIGDGMEFTIHGDLKNKAKKNKQIMNNSKDDDTASIKSYGSHKNRPFKDESHKGSAETMEGEEKRDASKEDLGIDEELDDEGEGDEGPLDGELIIHAEEDEVIEDSPADCCPDNCYKKFPVLAGDDDAPFWQGWGNLRLKTFQLIENKYFETAVITMILLSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFIIVMVSLINFVASLCGAGGIQAFKTMRTLRALRPLRAMSRMQGMRVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCVDKNKTTLPHEIIPDVNACKAENYSWENSPMNFDHVGKAYLCLFQVATFKGWIQIMNDAIDSRDVSFVGKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTNKKFDMIIMLFIGFNMLTMTLDHYKQSETFSAVLDYLNMIFICIFSSECLMKIFALRYHYFIEPWNLFDFVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKDKSGLDDVYNFKTFGQSMILLFQMSTSAGWDGVLDGIINEEDCLPPDNDKGYPGNCGSSTIGITYLLAYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPDGTQYVRYDQLSDFLDVLEPPLQIHKPNRYKIISMDIPICRGDMMFCVDILDALTKDFFARKGNPIEETAELGEVQQRPDEVGYEPVSSTLWRQREEYCARLIQHAWKRYKQRHGGGTDASGDDLEIDACDNGCGGGNGNENDDSGDGATGSGDNGSQHGGGSISGGGGTPGGGKSKGIIGSTQANIGIVDSNISPKESPDSIGDPQGRQTAVLVESDGFVTKNGHRVVIHSRSPSITSRTADV

Target ORF information:

RefSeq Version	XM_001689051.1
Organism	Anopheles gambiae str. PEST
Definition	Anopheles gambiae str. PEST AGAP004707-RB (AgaP_AGAP004707), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001689051.1

ORF Insert Sequence:

ATGACCGAAG ACTCCGATTC GATATCTGAG GAAGAACGTA GTTTGTTCCG TCCTTTCACT 
CGTGAATCAT TACAAGCTAT CGAAGCACGC ATTGCAGATG AAGAAGCCAA ACAGCGAGAA 
TTGGAAAGAA AACGAGCTGA GGGGGAGGAT GAGGATGAAG GTCCCCAACC GGACCCTACT 
CTTGAACAGG GTGTACCAGT CCCAGTTCGA ATGCAAGGCA GCTTCCCCCC GGAGTTGGCC 
TCCACGCCTC TCGAGGATAT TGACAGTTTC TATTCAAATC AAAGGACATT CGTAGTGATT 
AGTAAAGGAA AAGATATATT TCGTTTCTCC GCAACTAACG CATTATATGT ACTTGATCCG 
TTTAACCCCA TACGCCGCGT AGCTATTTAT ATTTTAGTAC ATCCACTGTT TTCACTTTTT 
ATAATAACGA CCATTCTTGT TAATTGTATA TTGATGATTA TGCCTACCAC GCCGACAGTC 
GAATCTACCG AGGTGATATT CACCGGCATC TACACGTTCG AATCAGCTGT AAAAGTGATG 
GCGCGAGGTT TCATATTACA ACCGTTTACT TATCTTAGAG ATGCATGGAA TTGGTTGGAC 
TTCGTAGTAA TAGCATTAGC ATATGTAACT ATGGGTATAG ATTTGGGTAA TCTTGCTGCG 
TTGAGAACAT TCAGGGTATT ACGAGCTCTT AAAACGGTAG CCATCGTTCC AGGCTTAAAA 
ACCATCGTCG GAGCCGTTAT AGAATCCGTA AAGAATCTCA GAGATGTGAT AATTTTAACA 
ATGTTTTCGT TATCCGTGTT TGCTTTGATG GGTCTACAAA TCTACATGGG AGTACTAACA 
CAAAAGTGCA TAAAAGAGTT CCCATTGGAT GGTTCCTGGG GTAATCTAAC CGACGAAAGC 
TGGGAGCTGT TCAACAGCAA TGACACAAAT TGGTTCTATT CCGAGAGTGG CGACATTCCT 
CTTTGTGGAA ACTCATCTGG AGCTGGACAA TGTGATGAAG GCTACATTTG TTTACAAGGC 
TATGGCAAAA ATCCAAATTA CGGGTATACA AGTTTTGATA CATTCGGATG GGCATTCTTG 
TCTGCCTTTC GTCTAATGAC TCAAGATTAT TGGGAGAATT TATATCAACT GGTGTTACGA 
TCAGCTGGAC CGTGGCACAT GCTCTTCTTC ATTGTGATTA TCTTCTTGGG TTCGTTTTAC 
CTTGTAAATT TAATCTTGGC CATTGTCGCC ATGTCGTACG ACGAACTCCA GAAGAAGGCC 
GAAGAGGAAG AGGCCGCCGA GGAAGAAGCA CTTCGGGAAG CAGAAGAAGC TGCGGCAGCA 
AAGGCAGCTA AACTGGAAGC ACAACAAGCG GCCGCAGCAG CAGCGGCGAA CCCAGAAATC 
GCTAAAAGCC CTTCGGATTT CTCCTGTCAC AGCTATGAGT TGTTTGTCGG ACAGGAGAAA 
GGCAACGACG ATAACAATAA GGAGAAAATG TCCATTAGAA GCGAAGGATT GGAGTCGGTG 
AGCGAAATCA CAAGAACAAC CGCACCAACA GCTACTGCAG CTGGCACTGC AAAAGCCCGT 
AAAGTGAGCG CGGCTTCACT TTCATTACCC GGTTCACCAT TTAATCTTCG TAGAGGATCT 
AGAGGATCAC ACCAGTTCAC GATACGTAAC GGTAGAGGAC GTTTCGTTGG TGTACCTGGT 
AGCGATAGAA AACCACTGGT ACTATCAACA TATCTCGATG CACAAGAACA CCTGCCATAC 
GCTGATGATT CCAACGCGGT AACGCCGATG TCGGAAGAAA ATGGTGCAAT CATCGTTCCA 
GTATACTATG CTAATTTAGG TTCAAGGCAC TCGTCGTATA CTTCGCATCA GTCGCGTATT 
TCGTACACAT CTCACGGTGA CCTGCTCGGG GGCATGACAA AAGAGAGCCG TCTGCGAAAT 
CGATCAGCCC GTAACACTAA CCATTCAATT GTACCACCTC CGAATGCTAA CAATCTATCC 
TACGCTGAAA CAAACCATAA AGGACAGCGA GATTTCGACT TGACACAGGA CTGTACAGAC 
GATGCCGGAA AAATAAAACA TAACGACAAT CCTTTCATAG AACCTGCTCA AACTCAAACT 
GTGGTAGATA TGAAAGACGT GATGGTGTTA AATGACATTA TTGAGCAAGC TGCTGGTCGG 
CACAGCAGGG CAAGTGATCA CGGAGTCTCT GTTTATTACT TCCCCACAGA GGACGACGAC 
GAAGATGGCC CAACGTTTAA GGACAAAGCC CTCGAGTTTC TGATGAAGAT GATCGACATT 
TTCTGTGTGT GGGACTGTTG TTGGGTTTGG CTTAAATTTC AGGAATGGGT TGCTTTTATT 
GTGTTTGACC CATTTGTAGA GCTATTCATT ACGCTCTGCA TTGTGGTAAA TACACTGTTT 
ATGGCTTTAG ATCATCACGA TATGGATCCA GATATGGAAA AGGCACTGAA AAGTGGCAAC 
TACTTCTTCA CAGCTACATT TGCCATCGAA GCTACAATGA AGCTCATAGC AATGAGCCCC 
AAATATTACT TTCAAGAGGG TTGGAATATC TTCGATTTTA TTATCGTAGC ACTGTCTCTG 
CTAGAATTGG GACTTGAGGG TGTTCAAGGA TTGTCAGTAT TACGATCGTT CCGTTTGCTT 
CGAGTTTTTA AGCTCGCTAA ATCGTGGCCA ACGCTGAATT TACTCATTTC CATCATGGGC 
AGAACGATGG GTGCGTTAGG TAATCTGACG TTCGTACTCT GCATTATCAT CTTCATCTTT 
GCCGTGATGG GAATGCAGCT GTTCGGAAAG AACTATGTCG ATAATGTGGA TAGATTCCCC 
GACCATGATC TGCCAAGATG GAATTTTACA GATTTCATGC ATTCCTTCAT GATTGTGTTC 
CGTGTGCTAT GCGGAGAATG GATTGAATCA ATGTGGGATT GTATGCTTGT CGGTGATGTA 
TCCTGCATAC CATTTTTCTT GGCCACTGTA GTGATAGGAA ATTTAGTCGT GCTTAACCTT 
TTCTTAGCCT TGCTTTTGTC AAATTTTGGT TCATCATCCT TGTCTGCACC AACGGCAGAT 
AATGAGACCA ACAAGATTGC AGAAGCGTTC AACAGAATAT CACGCTTTTC TAACTGGATT 
AAAATGAATT TAGCAAACGC TCTCAAGTTT GTAAAAAATA AATTAACAAG CCAAATAGCA 
TCCGTTCAAC CGACAGGCAA AGGCGTATGT CCATGTATAT CTTCAGAGCA TGGTGAAAAT 
GAACTGGAAC TTACTCCAGA CGATATTTTG GCGGATGGAC TATTGAAGAA AGGAATCAAA 
GAGCACAACC AACTGGAAGT AGCGATTGGC GATGGCATGG AATTCACCAT TCATGGTGAT 
CTGAAGAACA AAGCAAAAAA GAATAAACAA ATCATGAACA ACTCTAAGGA CGATGATACT 
GCTAGTATCA AATCTTATGG CAGTCACAAG AATCGCCCAT TTAAGGATGA AAGCCACAAA 
GGCAGCGCCG AAACGATGGA GGGTGAAGAA AAACGTGATG CCAGCAAGGA GGATCTAGGA 
ATTGACGAAG AACTCGACGA CGAAGGCGAG GGAGATGAAG GTCCTCTGGA TGGAGAGCTG 
ATTATTCATG CAGAAGAAGA CGAAGTGATT GAGGATTCAC CGGCGGATTG CTGCCCGGAC 
AACTGCTACA AAAAATTTCC TGTTCTTGCT GGGGATGATG ACGCGCCGTT CTGGCAGGGT 
TGGGGAAATT TACGTCTCAA AACGTTTCAG CTAATAGAAA ATAAGTATTT TGAGACAGCT 
GTAATTACAA TGATTCTGCT TAGTAGCTTA GCTTTGGCCC TCGAAGATGT GCATCTTCCA 
CAGCGCCCAA TCCTTCAAGA TATTCTTTAT TACATGGATC GAATTTTCAC AGTGATCTTT 
TTTTTAGAGA TGTTAATCAA ATGGTTAGCT TTAGGTTTTA AAGTATATTT TACAAATGCT 
TGGTGTTGGC TTGATTTCAT TATCGTGATG GTATCTTTGA TAAACTTCGT TGCTTCACTT 
TGTGGAGCTG GTGGTATTCA AGCATTCAAA ACCATGCGAA CTCTTAGAGC CCTGAGACCA 
CTACGTGCCA TGTCCCGTAT GCAGGGAATG AGGGTCGTCG TGAATGCGTT GGTTCAAGCT 
ATACCGTCCA TCTTCAACGT GCTGCTGGTT TGTTTGATAT TCTGGCTAAT ATTTGCAATC 
ATGGGGGTGC AATTATTTGC TGGCAAATAC TTCAAGTGTG TGGATAAAAA TAAAACTACA 
TTACCTCACG AAATTATACC GGATGTAAAT GCTTGCAAAG CCGAAAACTA TTCATGGGAA 
AATTCACCAA TGAACTTCGA TCATGTAGGT AAAGCATATT TGTGTCTGTT TCAAGTAGCC 
ACATTCAAAG GATGGATACA AATAATGAAC GATGCTATTG ATTCTAGAGA CGTAAGTTTT 
GTCGGTAAAC AGCCTATACG GGAAACGAAT ATCTACATGT ATCTGTACTT TGTGTTCTTT 
ATTATCTTTG GGTCATTCTT CACGTTGAAT CTATTCATTG GTGTTATAAT TGACAACTTC 
AATGAACAGA AAAAGAAAGC TGGTGGATCG CTAGAAATGT TCATGACAGA GGATCAGAAA 
AAGTACTATA ATGCAATGAA AAAAATGGGT TCGAAGAAAC CTCTAAAGGC AATTCCTCGT 
CCAAGGTGGC GGCCTCAAGC AATAGTTTTT GAAATAGTGA CGAACAAAAA GTTTGACATG 
ATTATCATGT TGTTCATCGG ATTCAATATG TTAACTATGA CACTGGACCA CTACAAACAA 
TCAGAAACTT TTAGTGCTGT TTTGGATTAC TTGAATATGA TATTCATCTG CATATTCAGC 
AGCGAATGTT TAATGAAGAT TTTTGCACTT CGTTATCATT ACTTTATCGA GCCATGGAAT 
TTGTTTGATT TTGTTGTCGT CATTCTTTCG ATTTTGGGCC TTGTTCTAAG TGATATCATT 
GAAAAATATT TTGTATCTCC CACACTTCTA CGAGTCGTGC GAGTGGCAAA AGTGGGCCGA 
GTATTGCGTT TGGTTAAAGG AGCCAAGGGT ATCCGAACGT TGCTGTTTGC ATTAGCAATG 
TCGCTACCTG CACTATTTAA CATCTGCTTG TTACTCTTTT TGGTGATGTT TATATTTGCC 
ATTTTTGGAA TGTCATTTTT CATGCACGTC AAAGATAAGA GTGGCTTAGA TGACGTGTAC 
AATTTTAAAA CGTTTGGCCA GAGCATGATT TTACTATTTC AGATGTCAAC CTCGGCTGGG 
TGGGATGGTG TTTTAGATGG TATTATCAAT GAAGAAGACT GTCTTCCACC AGACAATGAT 
AAGGGCTATC CGGGAAATTG TGGTTCATCA ACAATTGGCA TAACGTACTT ATTGGCGTAT 
CTTGTAATAA GTTTCCTTAT CGTTATTAAC ATGTACATTG CTGTTATCCT CGAAAACTAC 
TCGCAAGCTA CGGAAGATGT TCAAGAAGGC TTAACTGATG ACGATTATGA TATGTACTAC 
GAAATATGGC AGCAATTCGA TCCTGACGGT ACACAATACG TTCGATATGA TCAGCTATCA 
GACTTTTTGG ATGTGCTGGA ACCGCCTCTA CAGATTCATA AACCAAATCG TTATAAGATT 
ATTTCGATGG ATATTCCGAT ATGCCGCGGA GATATGATGT TCTGTGTCGA TATTCTAGAT 
GCACTAACGA AAGATTTTTT TGCTAGAAAA GGAAATCCTA TAGAAGAAAC AGCCGAATTA 
GGTGAAGTTC AACAACGCCC AGACGAAGTT GGTTACGAAC CAGTATCATC AACACTTTGG 
AGGCAGCGTG AAGAGTACTG TGCTCGACTG ATACAGCATG CGTGGAAACG CTATAAACAG 
CGTCACGGAG GCGGAACAGA CGCTTCAGGA GATGATCTTG AAATAGATGC CTGTGATAAC 
GGTTGTGGTG GTGGTAATGG CAATGAAAAT GATGATAGTG GAGATGGTGC AACAGGTAGT 
GGTGACAACG GAAGTCAGCA TGGTGGTGGC AGCATAAGTG GCGGAGGAGG AACTCCTGGT 
GGTGGTAAAA GTAAAGGAAT TATTGGCAGT ACTCAGGCTA ACATAGGCAT AGTGGATAGT 
AATATATCAC CAAAGGAATC ACCGGATAGC ATCGGCGATC CCCAAGGTCG TCAGACGGCC 
GTTCTTGTGG AGAGCGACGG ATTTGTGACG AAAAACGGTC ACCGTGTCGT CATACACTCT 
CGATCTCCCA GCATAACATC GCGAACGGCA GATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Anopheles gambiae genome: an update.
Trends in parasitology20(2)49-52(2004 Feb)
Mongin E,Louis C,Holt RA,Birney E,Collins FH

The genome sequence of the malaria mosquito Anopheles gambiae.
Science (New York, N.Y.)298(5591)129-49(2002 Oct)
Holt RA,Subramanian GM,Halpern A,Sutton GG,Charlab R,Nusskern DR,Wincker P,Clark AG,Ribeiro JM,Wides R,Salzberg SL,Loftus B,Yandell M,Majoros WH,Rusch DB,Lai Z,Kraft CL,Abril JF,Anthouard V,Arensburger P,Atkinson PW,Baden H,de Berardinis V,Baldwin D,Benes V,Biedler J,Blass C,Bolanos R,Boscus D,Barnstead M,Cai S,Center A,Chaturverdi K,Christophides GK,Chrystal MA,Clamp M,Cravchik A,Curwen V,Dana A,Delcher A,Dew I,Evans CA,Flanigan M,Grundschober-Freimoser A,Friedli L,Gu Z,Guan P,Guigo R,Hillenmeyer ME,Hladun SL,Hogan JR,Hong YS,Hoover J,Jaillon O,Ke Z,Kodira C,Kokoza E,Koutsos A,Letunic I,Levitsky A,Liang Y,Lin JJ,Lobo NF,Lopez JR,Malek JA,McIntosh TC,Meister S,Miller J,Mobarry C,Mongin E,Murphy SD,O'Brochta DA,Pfannkoch C,Qi R,Regier MA,Remington K,Shao H,Sharakhova MV,Sitter CD,Shetty J,Smith TJ,Strong R,Sun J,Thomasova D,Ton LQ,Topalis P,Tu Z,Unger MF,Walenz B,Wang A,Wang J,Wang M,Wang X,Woodford KJ,Wortman JR,Wu M,Yao A,Zdobnov EM,Zhang H,Zhao Q,Zhao S,Zhu SC,Zhimulev I,Coluzzi M,della Torre A,Roth CW,Louis C,Kalush F,Mural RJ,Myers EW,Adams MD,Smith HO,Broder S,Gardner MJ,Fraser CM,Birney E,Bork P,Brey PT,Venter JC,Weissenbach J,Kafatos FC,Collins FH,Hoffman SL

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AgaP_AGAP004707 cDNA ORF clone, Anopheles gambiae str. PEST

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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