AgaP_AGAP007417 cDNA ORF clone, Anopheles gambiae str. PEST

The following AgaP_AGAP007417 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AgaP_AGAP007417 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAh00773 XM_308421.4
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Anopheles gambiae str. PEST AGAP007417-RA (AgaP_AGAP007417), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OAh00773
    Clone ID Related Accession (Same CDS sequence) XM_308421.4
    Accession Version XM_308421.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-25
    Organism Anopheles gambiae str. PEST
    Product AGAP007417-PA
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_078265). On Oct 15, 2007 this sequence version replaced XM_308421.3. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCTACA GCTTGTTGGA CATTGCTGTT TCAAAGAAGA AGTCGGGAAA CAATCGTGTA 
    ATCTCCGATA GCTGCAACAC CGACGATAGT GATGAACCAT CAACATCCAG CATCCCACCG
    GCGGCACCAA CATTTACATA CAAAGGCAAG AAGTATACAC AGCTAGACCC GCAATTGAAA
    GTGAAACCAA TCCTACGCGA TGTCGAACGC ATTCCGGAGT ACCAGCTGAT AGAGAGAAAC
    GAGCCAGACG GTTGGACTCT CTCCATTACC CTGCGAATGC GACGGCGGGA TGAGCCCACC
    GATGATGGCT GCATCCGGCA CAGGCAGCGC ATGGTTGAGC TTTTGTTCGA AGAAATCGAC
    CGGCACTCGT TCATCGAGGA GGAGCTGGCG CAGAAACTAC CAACCTCACC GGAGGAAGAC
    AGCAACGAAC CGCTCTCCAT ACAAGAGTTT TACAATCGAT TACGATTGCG CCACCGAACG
    CAATCGGGTG ATAAAAGCTC GAAGGAATTG GTAATTCCAC TGTTGCGCCC CGAATTGCGC
    TTGTACCAAG AGCAAGCGAT CCGTTGGTTG CTAAAGCGGG AAACCGTCGT CGATCGATTG
    CCCGGCCAGT ACGTTCTGCT TCGTTGCCGT GCCCAGCCAG AGGTGAGCTT CTACATGGAT
    CTGTACGACT GTACGATAAG CGATAAGAAA CCGAACCCAA AAATACCCGC CGGTGGTATA
    CTGGCCGATG AGATGGGTAT GGGCAAAACG GTCGAAATCC TAGGCCTAAT GCTGCTGAAC
    CCGAAGAAAC AAGACTCGCC CAAGGCGCCC CAGGAGCCCC AGGAGCCCCA GGAGCCCCAA
    GATCCCAACG AAGTTAAACT TAAGGCGACG AAAAATGAAG GTGAACTAAG GTGCTTGTGT
    GCTAGCACAC TGACCAAGAA GACAATCGCC TGCCGCAAGT GCGGCCGGCT GCAGCATCGC
    AAGTGTGTTT TGAACCATTT TACCCAGCCA AACGAGCAGT ACATCTGTCC CGAGTGTTGG
    CGTACGCAAC CGATGGTCAA ATCCGGTGCT ACCATCATCG TATCGCCCGC CTCGATCAAG
    CACCAGTGGG AAAGCGAAAT CCGCAAGCAC GTGACCGATC CGAACTTTCG CGTGTTCATC
    TACAACGGCA TCGCGGACAA ATGGATCAGC CCGCAGGATC TCGCTGCGTA CGACGTCGTG
    CTGACGGACT ACACCGTGCT CAGTCCGGAA ATCTATCGCG TACCGGAGTA CGCTCGTAGC
    TCCCGGCACG AGCAGCGCTT CCTCAAACCG TCCACCCCGC TCACGATGAT CCACTGGTGG
    CGCGTCGTCC TGGACGAAGC GCAAATGGTC GAGTCGGTGA ACAATAACGC GGCCAAGATG
    GTCAAAGCAC TGCCGACCAA GCACCGGTGG GCCGTGACCG GTACGCCGAT CGAAAAGACG
    ATCAACAATC TGCACGGACT GGTCAGCTTC CTGGACTACG AGCCGTACTC TTACTGGCGC
    ACGTGGAAAG CGTACGCGGA ACGCTTCCAG CAGGGCAATG CCGAACCGCT GCTCACGATG
    ATGGCGCGCG TCATGTGGCG AACGTGCAAG CATTCCGTCT TCGATCAGCT GGACATTCCG
    CCGCAAACCG AGCGCATCCA CCACATCCAG ATGTCGGACC TGCAGAACTA CTTCTACCGC
    TGCGAGCATC TCGCCTGTGC GCAGGCATTC AACGAGAAGG CGCGCCGCAT CGGAGCCAAC
    GAGCGGATGG CACAGATGCA CATCGCCACG CTGAATCAGC TGCTGGAACC GCTGCGCAAG
    CTGCGGCAGG ATTGCACCAT CCCGTCCGTG TTGGGCGTGG GTCAGTCAGC ACTACAAGGG
    AAGAGACTGC TGACGCCGGC CGAACTGCAC GAGCATCTAA TCGCCTCCAA CGTTAACGAG
    TGCAAGGGCA AGCTGCGATC GGTCGTGTCG TCGCTAAACG GTATGGCGGC GGTCGAGATC
    CTGCAGGAGC ATTACGATCA AGCATTCCGG CTGTACCAGG CGAGTCTGCG GTGCGCCGAC
    GACTATCAGG GTGCGATCAG TGTGGACTCG CTGCTCCAGA TCCATGCGCT GCACAATCTG
    CTCGATCTGG TGCGTAGGTT TGAGGAGAAA CTAAGTCCCA GCTCGAAGCT TACGGTGGAA
    CATATCGCTG CGTACGAAGA GCGACGCTCC AAGCTGGAAG GACGCTACAT TGAGCAGTAC
    GCCAACAAGG TGCGTTCGAT CGAGGCTACA CTCCGTCCGG CGACGGAAAA GGTGGACGAA
    TTGATCAACG GCAATGATAG CTCCCAGCGG TTACAGCTCG AGGGAGAATG GTGGCGCGAT
    CTGTTCGCCC TGTTCGAGGA AAGTCCCCAG CGCCACTCGG CCATCCAAAC GCGCGTACTG
    CTCGACCAAA GCTTGATCGG CAACACGGGC GTCAATTCGT GGCGAACGCT TGACCTGTGG
    CTTGTGAACT GGTTGGATGG GCTGCTCGAA CGGCGCAGTT CCCTTGTGAA GGGCTTCCAA
    TCGTTGGGCT TCTTTGTGGA GTATTTGCAA CCGGACAGGA CGGACTGGTC GCAGGAGGCA
    CGTGCTCGAA TCGATGAGCT GGTACGGACG GCGTTCGCGT GCCATCTCGA TCCGGCTCTG
    TTCGAGGAGG ATGAGTTCGG AGTACGCAAG AAGGAGCGCC AAGAAGCGAA GGGCTTACCG
    GGTCCACCCG TTTGCTTACT GTGCAAGGTA AAGGACAAGC TGAATGAGCT GGAATCGGCA
    CTGTTCCAGA TTCGAAAGAC CCAAGTCGCC ACCGGCGGAT TGTGGCAGGT TTCGCGACAA
    GAATCAGTAC TTAAGCTGAT ACATGCGTAT GCAAAGCGAC TGGAACAGGA CCGTACCGTA
    TGGAGCAGGA TGGTGTCGCA AGACACGATC GTCGAAAGTG ATCGGTTCAT TGCGTTTCTC
    GAGCACGCTA AGGTCGAGTT TAAGGAGTAC TCACAGTATT GGGTGGAGAT TAACTACACC
    GTCGCTGCGT ACGACGAGCT GACCATGTGC AAGTCTCGCC TGCAGCTGCT CACACTAGCG
    GAACGGCGCG AGATAGAGAA GAAGAAGAAA AAACCGTCCC CTTTGCAAGT ACTGGAATGT
    GAACTGGCCG ATACGCTGGC CGAGCTGCAG CTCACCAAAG CGGAGGCAGA GCGTGATTTC
    GTACGGCTGA AGGGTACGCG CAAGTATCTC GAACATCTTG GCTCGCGAAA GGAGCTCGAA
    CCATGCCCGA TCTGTCACAG CATCCCGGAG GGCCGGTATG CCGTGCTGCA GTGCGGCCAT
    CATCTGTGTA GCATCTGTTT GATGCGCATT TTCCAGCTAG CTCGAGCGCA CGGGAACATG
    ACCACGTGTG GTATCTGCCG GCATGAGCAA CACGTGAAAG ACATCCAGTA CGTGCAGCCA
    ATCGATCCAA TGCACAAGAT CCGGGGCAAC TTTTCCAACA AAGTGTCCAA AATTGTGCAA
    ACGGCACTGG AACTGGTAGC GGAGGATCCG TCCGTTAAGA TCGTCATCTT CTCCCACTGG
    GAACCGATAC TGACCGAGGT GGGTGTGGCG CTCGCCGCGA ACGATATCAC GTTGCGCGAA
    AAGTCGGCCA AATTCTATCA GTGTGTGGCA GATTTTAAGG ATCCGGAGAA GGGAATTACC
    TGCCTGCTGC TGCCACTGCG CTTTGGTTCA AAGGGATTGA ATCTAACCGA GGCGACGCAC
    GTGTTTCTGG TCGAGCCGAT ACTGAACCCC GGGGAGGAGA TGCAAGCAAT CGGGCGCGTG
    CATCGTATCG GCCAGACGCG GCCCACGTTT GTGCATCGGT TCATTATGCT GAACACGATC
    GAGACGACGA TCCACGAGAC GATTCAGGGC GATCGGAGCG GCCGATGGTG GTCGAAGGAT
    GTTACCGTAG AGCAGCTGGC GCAGCTATTT CGGCTGGACC AAGACGACGG CAGTGAGCTG
    CCCGCACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_308421.4
    CDS1..3972
    Translation

    Target ORF information:

    RefSeq Version XM_308421.4
    Organism Anopheles gambiae str. PEST
    Definition Anopheles gambiae str. PEST AGAP007417-RA (AgaP_AGAP007417), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_308421.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCTACA GCTTGTTGGA CATTGCTGTT TCAAAGAAGA AGTCGGGAAA CAATCGTGTA 
    ATCTCCGATA GCTGCAACAC CGACGATAGT GATGAACCAT CAACATCCAG CATCCCACCG
    GCGGCACCAA CATTTACATA CAAAGGCAAG AAGTATACAC AGCTAGACCC GCAATTGAAA
    GTGAAACCAA TCCTACGCGA TGTCGAACGC ATTCCGGAGT ACCAGCTGAT AGAGAGAAAC
    GAGCCAGACG GTTGGACTCT CTCCATTACC CTGCGAATGC GACGGCGGGA TGAGCCCACC
    GATGATGGCT GCATCCGGCA CAGGCAGCGC ATGGTTGAGC TTTTGTTCGA AGAAATCGAC
    CGGCACTCGT TCATCGAGGA GGAGCTGGCG CAGAAACTAC CAACCTCACC GGAGGAAGAC
    AGCAACGAAC CGCTCTCCAT ACAAGAGTTT TACAATCGAT TACGATTGCG CCACCGAACG
    CAATCGGGTG ATAAAAGCTC GAAGGAATTG GTAATTCCAC TGTTGCGCCC CGAATTGCGC
    TTGTACCAAG AGCAAGCGAT CCGTTGGTTG CTAAAGCGGG AAACCGTCGT CGATCGATTG
    CCCGGCCAGT ACGTTCTGCT TCGTTGCCGT GCCCAGCCAG AGGTGAGCTT CTACATGGAT
    CTGTACGACT GTACGATAAG CGATAAGAAA CCGAACCCAA AAATACCCGC CGGTGGTATA
    CTGGCCGATG AGATGGGTAT GGGCAAAACG GTCGAAATCC TAGGCCTAAT GCTGCTGAAC
    CCGAAGAAAC AAGACTCGCC CAAGGCGCCC CAGGAGCCCC AGGAGCCCCA GGAGCCCCAA
    GATCCCAACG AAGTTAAACT TAAGGCGACG AAAAATGAAG GTGAACTAAG GTGCTTGTGT
    GCTAGCACAC TGACCAAGAA GACAATCGCC TGCCGCAAGT GCGGCCGGCT GCAGCATCGC
    AAGTGTGTTT TGAACCATTT TACCCAGCCA AACGAGCAGT ACATCTGTCC CGAGTGTTGG
    CGTACGCAAC CGATGGTCAA ATCCGGTGCT ACCATCATCG TATCGCCCGC CTCGATCAAG
    CACCAGTGGG AAAGCGAAAT CCGCAAGCAC GTGACCGATC CGAACTTTCG CGTGTTCATC
    TACAACGGCA TCGCGGACAA ATGGATCAGC CCGCAGGATC TCGCTGCGTA CGACGTCGTG
    CTGACGGACT ACACCGTGCT CAGTCCGGAA ATCTATCGCG TACCGGAGTA CGCTCGTAGC
    TCCCGGCACG AGCAGCGCTT CCTCAAACCG TCCACCCCGC TCACGATGAT CCACTGGTGG
    CGCGTCGTCC TGGACGAAGC GCAAATGGTC GAGTCGGTGA ACAATAACGC GGCCAAGATG
    GTCAAAGCAC TGCCGACCAA GCACCGGTGG GCCGTGACCG GTACGCCGAT CGAAAAGACG
    ATCAACAATC TGCACGGACT GGTCAGCTTC CTGGACTACG AGCCGTACTC TTACTGGCGC
    ACGTGGAAAG CGTACGCGGA ACGCTTCCAG CAGGGCAATG CCGAACCGCT GCTCACGATG
    ATGGCGCGCG TCATGTGGCG AACGTGCAAG CATTCCGTCT TCGATCAGCT GGACATTCCG
    CCGCAAACCG AGCGCATCCA CCACATCCAG ATGTCGGACC TGCAGAACTA CTTCTACCGC
    TGCGAGCATC TCGCCTGTGC GCAGGCATTC AACGAGAAGG CGCGCCGCAT CGGAGCCAAC
    GAGCGGATGG CACAGATGCA CATCGCCACG CTGAATCAGC TGCTGGAACC GCTGCGCAAG
    CTGCGGCAGG ATTGCACCAT CCCGTCCGTG TTGGGCGTGG GTCAGTCAGC ACTACAAGGG
    AAGAGACTGC TGACGCCGGC CGAACTGCAC GAGCATCTAA TCGCCTCCAA CGTTAACGAG
    TGCAAGGGCA AGCTGCGATC GGTCGTGTCG TCGCTAAACG GTATGGCGGC GGTCGAGATC
    CTGCAGGAGC ATTACGATCA AGCATTCCGG CTGTACCAGG CGAGTCTGCG GTGCGCCGAC
    GACTATCAGG GTGCGATCAG TGTGGACTCG CTGCTCCAGA TCCATGCGCT GCACAATCTG
    CTCGATCTGG TGCGTAGGTT TGAGGAGAAA CTAAGTCCCA GCTCGAAGCT TACGGTGGAA
    CATATCGCTG CGTACGAAGA GCGACGCTCC AAGCTGGAAG GACGCTACAT TGAGCAGTAC
    GCCAACAAGG TGCGTTCGAT CGAGGCTACA CTCCGTCCGG CGACGGAAAA GGTGGACGAA
    TTGATCAACG GCAATGATAG CTCCCAGCGG TTACAGCTCG AGGGAGAATG GTGGCGCGAT
    CTGTTCGCCC TGTTCGAGGA AAGTCCCCAG CGCCACTCGG CCATCCAAAC GCGCGTACTG
    CTCGACCAAA GCTTGATCGG CAACACGGGC GTCAATTCGT GGCGAACGCT TGACCTGTGG
    CTTGTGAACT GGTTGGATGG GCTGCTCGAA CGGCGCAGTT CCCTTGTGAA GGGCTTCCAA
    TCGTTGGGCT TCTTTGTGGA GTATTTGCAA CCGGACAGGA CGGACTGGTC GCAGGAGGCA
    CGTGCTCGAA TCGATGAGCT GGTACGGACG GCGTTCGCGT GCCATCTCGA TCCGGCTCTG
    TTCGAGGAGG ATGAGTTCGG AGTACGCAAG AAGGAGCGCC AAGAAGCGAA GGGCTTACCG
    GGTCCACCCG TTTGCTTACT GTGCAAGGTA AAGGACAAGC TGAATGAGCT GGAATCGGCA
    CTGTTCCAGA TTCGAAAGAC CCAAGTCGCC ACCGGCGGAT TGTGGCAGGT TTCGCGACAA
    GAATCAGTAC TTAAGCTGAT ACATGCGTAT GCAAAGCGAC TGGAACAGGA CCGTACCGTA
    TGGAGCAGGA TGGTGTCGCA AGACACGATC GTCGAAAGTG ATCGGTTCAT TGCGTTTCTC
    GAGCACGCTA AGGTCGAGTT TAAGGAGTAC TCACAGTATT GGGTGGAGAT TAACTACACC
    GTCGCTGCGT ACGACGAGCT GACCATGTGC AAGTCTCGCC TGCAGCTGCT CACACTAGCG
    GAACGGCGCG AGATAGAGAA GAAGAAGAAA AAACCGTCCC CTTTGCAAGT ACTGGAATGT
    GAACTGGCCG ATACGCTGGC CGAGCTGCAG CTCACCAAAG CGGAGGCAGA GCGTGATTTC
    GTACGGCTGA AGGGTACGCG CAAGTATCTC GAACATCTTG GCTCGCGAAA GGAGCTCGAA
    CCATGCCCGA TCTGTCACAG CATCCCGGAG GGCCGGTATG CCGTGCTGCA GTGCGGCCAT
    CATCTGTGTA GCATCTGTTT GATGCGCATT TTCCAGCTAG CTCGAGCGCA CGGGAACATG
    ACCACGTGTG GTATCTGCCG GCATGAGCAA CACGTGAAAG ACATCCAGTA CGTGCAGCCA
    ATCGATCCAA TGCACAAGAT CCGGGGCAAC TTTTCCAACA AAGTGTCCAA AATTGTGCAA
    ACGGCACTGG AACTGGTAGC GGAGGATCCG TCCGTTAAGA TCGTCATCTT CTCCCACTGG
    GAACCGATAC TGACCGAGGT GGGTGTGGCG CTCGCCGCGA ACGATATCAC GTTGCGCGAA
    AAGTCGGCCA AATTCTATCA GTGTGTGGCA GATTTTAAGG ATCCGGAGAA GGGAATTACC
    TGCCTGCTGC TGCCACTGCG CTTTGGTTCA AAGGGATTGA ATCTAACCGA GGCGACGCAC
    GTGTTTCTGG TCGAGCCGAT ACTGAACCCC GGGGAGGAGA TGCAAGCAAT CGGGCGCGTG
    CATCGTATCG GCCAGACGCG GCCCACGTTT GTGCATCGGT TCATTATGCT GAACACGATC
    GAGACGACGA TCCACGAGAC GATTCAGGGC GATCGGAGCG GCCGATGGTG GTCGAAGGAT
    GTTACCGTAG AGCAGCTGGC GCAGCTATTT CGGCTGGACC AAGACGACGG CAGTGAGCTG
    CCCGCACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Anopheles gambiae genome: an update.
    Trends in parasitology20(2)49-52(2004 Feb)
    Mongin E,Louis C,Holt RA,Birney E,Collins FH


    The genome sequence of the malaria mosquito Anopheles gambiae.
    Science (New York, N.Y.)298(5591)129-49(2002 Oct)
    Holt RA,Subramanian GM,Halpern A,Sutton GG,Charlab R,Nusskern DR,Wincker P,Clark AG,Ribeiro JM,Wides R,Salzberg SL,Loftus B,Yandell M,Majoros WH,Rusch DB,Lai Z,Kraft CL,Abril JF,Anthouard V,Arensburger P,Atkinson PW,Baden H,de Berardinis V,Baldwin D,Benes V,Biedler J,Blass C,Bolanos R,Boscus D,Barnstead M,Cai S,Center A,Chaturverdi K,Christophides GK,Chrystal MA,Clamp M,Cravchik A,Curwen V,Dana A,Delcher A,Dew I,Evans CA,Flanigan M,Grundschober-Freimoser A,Friedli L,Gu Z,Guan P,Guigo R,Hillenmeyer ME,Hladun SL,Hogan JR,Hong YS,Hoover J,Jaillon O,Ke Z,Kodira C,Kokoza E,Koutsos A,Letunic I,Levitsky A,Liang Y,Lin JJ,Lobo NF,Lopez JR,Malek JA,McIntosh TC,Meister S,Miller J,Mobarry C,Mongin E,Murphy SD,O'Brochta DA,Pfannkoch C,Qi R,Regier MA,Remington K,Shao H,Sharakhova MV,Sitter CD,Shetty J,Smith TJ,Strong R,Sun J,Thomasova D,Ton LQ,Topalis P,Tu Z,Unger MF,Walenz B,Wang A,Wang J,Wang M,Wang X,Woodford KJ,Wortman JR,Wu M,Yao A,Zdobnov EM,Zhang H,Zhao Q,Zhao S,Zhu SC,Zhimulev I,Coluzzi M,della Torre A,Roth CW,Louis C,Kalush F,Mural RJ,Myers EW,Adams MD,Smith HO,Broder S,Gardner MJ,Fraser CM,Birney E,Bork P,Brey PT,Venter JC,Weissenbach J,Kafatos FC,Collins FH,Hoffman SL