SHPRH cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SHPRH
Entrez Gene ID	476233
Full Name	SNF2 histone linker PHD RING helicase
General protein information	Preferred Names SNF2 histone linker PHD RING helicase Names E3 ubiquitin-protein ligase SHPRH
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005615488.3	XP_005615545.1	E3 ubiquitin-protein ligase SHPRH
XM_005615487.2	XP_005615544.1	E3 ubiquitin-protein ligase SHPRH isoform X1
XM_005615489.2	XP_005615546.1	E3 ubiquitin-protein ligase SHPRH isoform X2
XM_005615488.2	XP_005615545.1	E3 ubiquitin-protein ligase SHPRH isoform X1
XM_005615487.3	XP_005615544.1	E3 ubiquitin-protein ligase SHPRH
XM_533438.5	XP_533438.2	E3 ubiquitin-protein ligase SHPRH
XM_533438.4	XP_533438.2	E3 ubiquitin-protein ligase SHPRH isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SHPRH	XP_001172976.2
Bos taurus (cattle)	SHPRH	XP_002690346.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007417	XP_308421.4
Homo sapiens (human)	SHPRH	NP_001036148.2
Mus musculus (house mouse)	Shprh	NP_001071175.1
Gallus gallus (chicken)	SHPRH	XP_419651.3
	SHPRH	XP_001086512.1
Danio rerio (zebrafish)	shprh	XP_003200358.1
Drosophila melanogaster (fruit fly)	CG7376	NP_648034.1
Canis lupus familiaris (dog)	SHPRH	XP_533438.2
Rattus norvegicus (Norway rat)	Shprh	NP_001100940.1
Xenopus tropicalis (tropical clawed frog)	shprh	XP_004914950.1

Gene Ontology

The following SHPRH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHPRH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe00920	XM_005615489.2 Latest version!	Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCe00919	XM_005615487.3 Latest version!	Canis lupus familiaris SNF2 histone linker PHD RING helicase (SHPRH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe00919	XM_533438.5 Latest version!	Canis lupus familiaris SNF2 histone linker PHD RING helicase (SHPRH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe00919	XM_005615488.3 Latest version!	Canis lupus familiaris SNF2 histone linker PHD RING helicase (SHPRH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCe00919	XM_005615488.2	Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe00919	XM_005615487.2	Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe00919	XM_533438.4	Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHPRH ( XM_005615489.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005615546.1 Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X4, mRNA.

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CloneID	OCe00920
Clone ID Related Accession (Same CDS sequence)	XM_005615489.2
Accession Version	XM_005615489.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase SHPRH isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615489.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAT TCAATCAACT TATGAAAAGA GTGATGGAAA AGTTATACAA TTTTGTTATT 
CCAGATGTGT TGGAAGAGGA TGAGGAAGAG TCAGAGAGTG AGCCAGAGGG ACAGGAGATT 
GATGAACTCT ATCAGTTTGT GAAGCAAACA CATCAGCAAG AAACACAGTC CATCCAAGTG 
GATGTCCAGC ATCCTGCCTT GATTCCTGTG TTGAGACCAT ACCAGAGAGA GGCTGTCAAT 
TGGATGCTAC AACAAGAGCA TTTCAGAAGT GCTCCTACTA ATGAAAATGC CTTGCACTTC 
TTATGGCGTG AGATTGTTAC ACCTGAGGAT TTGAAACTCT ACTACAATCC ATATACAGGC 
TGCATCATCC GTGATTACCC AAATGCTGGG CCACAGTTGC TTGGTGGAAT CTTAGCAGAT 
GAGATGGGTC TCGGAAAGAC AGTGGAGGTT TTGGCTCTGA TTCTGACACA TACTCGACAA 
GATGTCAAAC AAGATGCTCT CACTCTTCCT GAGGGAAAAG TGGTGAATTA TTTCATTCCA 
TCACATTATT CTGGAGGAAA AGTAAAAAAC ACAGAAACCC AGAATATGGA ATTTGAACCA 
AAAGAAAAAG TTCAGTGCCC CCCTACACGT GTGATGATCC TGACAGCTGT GAAAGAAATG 
AATGGAAAAA AAGGAGTTTC CATCCTTTCC ATCTATAAAT ATGTCAGTTC TATATATAGA 
TATGATGTTC AGCGGAACAG GAGTCTTCTG AAACGGATGC TGAAATGTTT AATTTTTGAA 
GGTCTTGTGA AACAGATCAA AGGCCATGGT TTTTCTGGAA CTTTTACATT GGGGAAGAAT 
TACAAGGAAG AGGATATATA TGATAAAACA AAAAAGCAGG CAGTAGGGAG TCCAAGGAAA 
ATTCAGAAAG AATCCAGAAA ATCAGGGAGC AAAGACACAG ATTCTGAATA CTTGCCATCA 
AACACATCTG ATGATGATGA TGATCCTTAC TATTATTATT ATAAGGCTCG GAGAAATCGT 
AGTAAATTCA GGAAAAAGCC TGTTACATCC ACAAAAAAGG GAAAAGGTCA GCCTAATATC 
AATCTCAGTT CACAAGATCA CTGTCCAGCT ACCAATGACT CTGGAATTAC CGATGTTGCC 
ACATCTAAAA GTGCATGTAT CTTTGAAGGC AAGCAAGAAC ATGCAGCTGA GGACCAAGCT 
GAATCTCTAA ACCCTGCTGA TGGTGATGTG CCACAATCTA CTATCTTGAG TCCCTGTAAT 
TCCTCTGATT ATCGCTTTGA ATGCATATGT GGTGAACTTG ACCAAGTAGA CCGTAAACCT 
CGTGTTCAGT GCCTGAAATG CCATCTGTGG CAACATGCAA AATGTGTGAA TTATGAAGAG 
AAGAATCTGA AGATTAAACC TTTTTACTGT CCCCACTGTC TTGTTGCAAT GGAGCCAGTA 
TCAACAAGAG CAACTCTGAT CATCTCGCCC AGTTCCATCT GTCATCAGTG GGTGGATGAA 
ATCAACAGGC ATGTGAGGTC ATCATCTCTT CGAGTCTTGG TATACCAAGG AGTGAAGAAA 
GATGGTTTTT TACAACCTCA TTTTTTGGCT GAACAGGATA TAGTTATCAT TACCTATGAT 
GTCCTTCGTT CAGAACTAAA CTATGTAGAT ATCCCACACA GCAATAGTGA GGATGGACGT 
CGCTTACGGA ACCAGAAGCG CTATATGGCC ATTCCCAGCC CCCTAGTAGC TGTGGAGTGG 
TGGAGGATCT GCCTTGATGA AGCCCAGATG GTTGAATGTC CTGCTGTGAA AGCTGCAGAA 
ATGGCTCAGC GCTTGAGTGG GATTAATCGC TGGTGCATCA GTGGCACTCC AGTGCAAAGA 
GGATTGGAGG ATCTTTTTGG GTTGGTGGTC TTTCTTGGTA TTGAACCTTA CTGTGTCAAA 
CACTGGTGGG TTCGACTTCT CTATCGCCCT TACTGCAAGA AGAATCCTCA GCATCTCTAC 
AGCTTTATTG CCAAGATACT ATGGAGGTCT GCAAAGAAGG ATGTGATTGA CCAAATCCAG 
ATACCCCCTC AGACAGAAGA AATCCACTGG CTTCACTTTT CTCCAGTGGA AAGACATTTC 
TATCACCGTC AGCATGAAGT GTGCTGCCAG GATGCTGTAG TAAAACTCAG GAAGATTTCA 
GACTGGGCGC TGAAGCTCAG CAGCCTGGAC AGAAGGACTG TCACCTCTAT CCTATATCCA 
TTGCTGAGAC TCAGGCAGGC CTGCTGTCAT CCACAGGCTG TTCGTGGGGA GTTCTTGCCA 
CTCCAGAAAA GCACCATGAC AATGGAAGAG CTGCTGACAT CTCTGCAGAA GAAATGTGGA 
ACTGAATGTG AAGAAGCACA TCGGCAACTA GTTTGTGCTC TCAATGGCTT AGCCGGCATC 
CACATCATTA AAGGTGAGTA TGCCTTGGCA GCAGAACTGT ACAGAGAAGT ATTGCGTTCA 
TCTGAGGAAC ACAAAGGAAA ACTCAAAACA GATTCACTCC AAAGACTACA TGCTACCCAT 
AACTTGATGG AATTGTTGGT AGCCAAGCAC CCAGGAATAC CTCCTACCTT ACGAGATGGC 
AGACTTGAGG AAGAGGCTAA ACAACTGCGA GAGCATTATA TGAGCAAGTG TAATACAGAA 
GTCGCTGAAG CTCAGCAAGC TTTACAGCCC GTGCAGCAGA CCATAAAGGA GCTCCAGAGA 
AAGATTTATT CTAATTCTCC TTGGTGGCTG AATGTGATCC ACAGAGCAAT AGAATTTGCT 
ATTGATGAGG AACTTGCTCA GCGAGTGCGA AATGAAATAA CAAGCAACTA CAAGCAACAA 
ATTGGCAAGC TTTCTATGTC AGAGAAGTTC CACGACTGTA GAGGTCTTCA GTTCTTACTT 
ACAACACAAA TGGAAGAGCT AAATAAATTC CAGAAGCTAG TAAGAGAGGC TGTAAAAAAC 
CTGGAGGGAC CTCCATCTCG TAATGTTATT GAATCGGCGA CAATCTGCCA CCTTCGACCA 
ACCAGACTTC CTCTCAACTG CTGTGTCTTT TGTAAGGCTG ATGAATTGTT CACAGAATAT 
GAATCAAAGC TGTTTTCCCA CACAGTCAAA GGCCAGACTG CAATATTTGA GGAGATGATA 
GAGGATGAAG AAGGATTGGT GGATGATCGA ATACCCACCA CAAGCCGGGG TCTGTGGGCA 
ATAAGTGAAA CAGAGCGGTC TCTGAAAGCA TTGCTCTCAT TTGCAAAATC ACATAGATTT 
GATGTTGAAT TCATTGATGA AGGAAGTACT TCAATGGACC TTTTTGAAGC ATGGAAAAAG 
GAATATAAGT TGCTTCATGA ATATTGGATG GCTCTGAGGA ATCGCGTGTC TGCTATTGAT 
GAACTTGCAA TGGCTACAGA ACGACTAAGA GTACGTCATC CTAGGGAGCC AAAGCCCAAC 
CCACCTGTTC TTCATATCAT TGAACCACAT GAGGTAGAAC AAAATCGTAT AAAACTACTA 
AATGATAAAG CCGTTGCTAC TTCACAGCTT CAGAAAAAAC TTGGGCAGCT TCTTTACCTA 
ACTAACTTGG AAAAGTCTCA AGATAAAACA TCAGGAGGGA TTAATCCAGA ACCTTGTCCA 
ATCTGTGCTC GGCAGCTGGG AAAACAGTGG GCAGTACTGA CCTGTGGACA CTGTTTCTGT 
AATGAGTGCA TTTCAATTAT AATTGAACAG TACAGCGTGG GGTCTCACAG AAGTTCCATT 
AAGTGTGCCA TCTGCCGCCA GACCACATCT CACAAAGAAA TCTCATATGT CTTCACCTCA 
GAGAAAGCCA GCCAGGAAGA AGACATCCCT GTGAAGGGCA GCCATTCTAC AAAAGTGGAA 
GCTGTGGTCA GAACTCTGAT GAGAATCCAA CTTAGAGACC CAGGGGCTAA AGCACTCGTC 
TTCTCAACGT GGCAAGATGT ATTAGATATT ATTTCAAAAG CCCTCACTGA CAACAACATG 
GAATTTGCTC AAATCAGTCG TGTTAAGACA TTTCAGGAGA ACCTTTCAGC ATTTAAACAT 
GATCCCCAAA TCAATATCTT GCTGCTGCCC CTGCACACAG GTTCTAATGG ATTAACTATC 
ATTGAAGCAA CTCATGTTCT CTTGGTGGAG CCCATCTTGA ACCCTGCCCA TGAGCTTCAG 
GCCATTGGGA GGGTGCACCG GATTGGACAA ACAAAACCTA CTATTGTACA CAGATTCTTA 
ATTAAAGCAA CAATAGAAGA AAGAATGCAG GCAATGCTGA AAACTGCTGA GAAGAGTCAC 
ACGAGCTCAT CGGTGAAGCA TCCCGAAGCT TCCGTCTTAA CCTTGGCTGA TCTGGCGGAT 
CTGTTTACCA AAGAAACTGA AGAACTTGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005615546.1
CDS	274..4626
Translation	MKKFNQLMKRVMEKLYNFVIPDVLEEDEEESESEPEGQEIDELYQFVKQTHQQETQSIQVDVQHPALIPVLRPYQREAVNWMLQQEHFRSAPTNENALHFLWREIVTPEDLKLYYNPYTGCIIRDYPNAGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPSHYSGGKVKNTETQNMEFEPKEKVQCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIYRYDVQRNRSLLKRMLKCLIFEGLVKQIKGHGFSGTFTLGKNYKEEDIYDKTKKQAVGSPRKIQKESRKSGSKDTDSEYLPSNTSDDDDDPYYYYYKARRNRSKFRKKPVTSTKKGKGQPNINLSSQDHCPATNDSGITDVATSKSACIFEGKQEHAAEDQAESLNPADGDVPQSTILSPCNSSDYRFECICGELDQVDRKPRVQCLKCHLWQHAKCVNYEEKNLKIKPFYCPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKDGFLQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPAVKAAEMAQRLSGINRWCISGTPVQRGLEDLFGLVVFLGIEPYCVKHWWVRLLYRPYCKKNPQHLYSFIAKILWRSAKKDVIDQIQIPPQTEEIHWLHFSPVERHFYHRQHEVCCQDAVVKLRKISDWALKLSSLDRRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLVAKHPGIPPTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQTIKELQRKIYSNSPWWLNVIHRAIEFAIDEELAQRVRNEITSNYKQQIGKLSMSEKFHDCRGLQFLLTTQMEELNKFQKLVREAVKNLEGPPSRNVIESATICHLRPTRLPLNCCVFCKADELFTEYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRIPTTSRGLWAISETERSLKALLSFAKSHRFDVEFIDEGSTSMDLFEAWKKEYKLLHEYWMALRNRVSAIDELAMATERLRVRHPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSGGINPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSEKASQEEDIPVKGSHSTKVEAVVRTLMRIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKHDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAEKSHTSSSVKHPEASVLTLADLADLFTKETEELE

Target ORF information:

RefSeq Version	XM_005615489.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615489.2

ORF Insert Sequence:

ATGAAGAAAT TCAATCAACT TATGAAAAGA GTGATGGAAA AGTTATACAA TTTTGTTATT 
CCAGATGTGT TGGAAGAGGA TGAGGAAGAG TCAGAGAGTG AGCCAGAGGG ACAGGAGATT 
GATGAACTCT ATCAGTTTGT GAAGCAAACA CATCAGCAAG AAACACAGTC CATCCAAGTG 
GATGTCCAGC ATCCTGCCTT GATTCCTGTG TTGAGACCAT ACCAGAGAGA GGCTGTCAAT 
TGGATGCTAC AACAAGAGCA TTTCAGAAGT GCTCCTACTA ATGAAAATGC CTTGCACTTC 
TTATGGCGTG AGATTGTTAC ACCTGAGGAT TTGAAACTCT ACTACAATCC ATATACAGGC 
TGCATCATCC GTGATTACCC AAATGCTGGG CCACAGTTGC TTGGTGGAAT CTTAGCAGAT 
GAGATGGGTC TCGGAAAGAC AGTGGAGGTT TTGGCTCTGA TTCTGACACA TACTCGACAA 
GATGTCAAAC AAGATGCTCT CACTCTTCCT GAGGGAAAAG TGGTGAATTA TTTCATTCCA 
TCACATTATT CTGGAGGAAA AGTAAAAAAC ACAGAAACCC AGAATATGGA ATTTGAACCA 
AAAGAAAAAG TTCAGTGCCC CCCTACACGT GTGATGATCC TGACAGCTGT GAAAGAAATG 
AATGGAAAAA AAGGAGTTTC CATCCTTTCC ATCTATAAAT ATGTCAGTTC TATATATAGA 
TATGATGTTC AGCGGAACAG GAGTCTTCTG AAACGGATGC TGAAATGTTT AATTTTTGAA 
GGTCTTGTGA AACAGATCAA AGGCCATGGT TTTTCTGGAA CTTTTACATT GGGGAAGAAT 
TACAAGGAAG AGGATATATA TGATAAAACA AAAAAGCAGG CAGTAGGGAG TCCAAGGAAA 
ATTCAGAAAG AATCCAGAAA ATCAGGGAGC AAAGACACAG ATTCTGAATA CTTGCCATCA 
AACACATCTG ATGATGATGA TGATCCTTAC TATTATTATT ATAAGGCTCG GAGAAATCGT 
AGTAAATTCA GGAAAAAGCC TGTTACATCC ACAAAAAAGG GAAAAGGTCA GCCTAATATC 
AATCTCAGTT CACAAGATCA CTGTCCAGCT ACCAATGACT CTGGAATTAC CGATGTTGCC 
ACATCTAAAA GTGCATGTAT CTTTGAAGGC AAGCAAGAAC ATGCAGCTGA GGACCAAGCT 
GAATCTCTAA ACCCTGCTGA TGGTGATGTG CCACAATCTA CTATCTTGAG TCCCTGTAAT 
TCCTCTGATT ATCGCTTTGA ATGCATATGT GGTGAACTTG ACCAAGTAGA CCGTAAACCT 
CGTGTTCAGT GCCTGAAATG CCATCTGTGG CAACATGCAA AATGTGTGAA TTATGAAGAG 
AAGAATCTGA AGATTAAACC TTTTTACTGT CCCCACTGTC TTGTTGCAAT GGAGCCAGTA 
TCAACAAGAG CAACTCTGAT CATCTCGCCC AGTTCCATCT GTCATCAGTG GGTGGATGAA 
ATCAACAGGC ATGTGAGGTC ATCATCTCTT CGAGTCTTGG TATACCAAGG AGTGAAGAAA 
GATGGTTTTT TACAACCTCA TTTTTTGGCT GAACAGGATA TAGTTATCAT TACCTATGAT 
GTCCTTCGTT CAGAACTAAA CTATGTAGAT ATCCCACACA GCAATAGTGA GGATGGACGT 
CGCTTACGGA ACCAGAAGCG CTATATGGCC ATTCCCAGCC CCCTAGTAGC TGTGGAGTGG 
TGGAGGATCT GCCTTGATGA AGCCCAGATG GTTGAATGTC CTGCTGTGAA AGCTGCAGAA 
ATGGCTCAGC GCTTGAGTGG GATTAATCGC TGGTGCATCA GTGGCACTCC AGTGCAAAGA 
GGATTGGAGG ATCTTTTTGG GTTGGTGGTC TTTCTTGGTA TTGAACCTTA CTGTGTCAAA 
CACTGGTGGG TTCGACTTCT CTATCGCCCT TACTGCAAGA AGAATCCTCA GCATCTCTAC 
AGCTTTATTG CCAAGATACT ATGGAGGTCT GCAAAGAAGG ATGTGATTGA CCAAATCCAG 
ATACCCCCTC AGACAGAAGA AATCCACTGG CTTCACTTTT CTCCAGTGGA AAGACATTTC 
TATCACCGTC AGCATGAAGT GTGCTGCCAG GATGCTGTAG TAAAACTCAG GAAGATTTCA 
GACTGGGCGC TGAAGCTCAG CAGCCTGGAC AGAAGGACTG TCACCTCTAT CCTATATCCA 
TTGCTGAGAC TCAGGCAGGC CTGCTGTCAT CCACAGGCTG TTCGTGGGGA GTTCTTGCCA 
CTCCAGAAAA GCACCATGAC AATGGAAGAG CTGCTGACAT CTCTGCAGAA GAAATGTGGA 
ACTGAATGTG AAGAAGCACA TCGGCAACTA GTTTGTGCTC TCAATGGCTT AGCCGGCATC 
CACATCATTA AAGGTGAGTA TGCCTTGGCA GCAGAACTGT ACAGAGAAGT ATTGCGTTCA 
TCTGAGGAAC ACAAAGGAAA ACTCAAAACA GATTCACTCC AAAGACTACA TGCTACCCAT 
AACTTGATGG AATTGTTGGT AGCCAAGCAC CCAGGAATAC CTCCTACCTT ACGAGATGGC 
AGACTTGAGG AAGAGGCTAA ACAACTGCGA GAGCATTATA TGAGCAAGTG TAATACAGAA 
GTCGCTGAAG CTCAGCAAGC TTTACAGCCC GTGCAGCAGA CCATAAAGGA GCTCCAGAGA 
AAGATTTATT CTAATTCTCC TTGGTGGCTG AATGTGATCC ACAGAGCAAT AGAATTTGCT 
ATTGATGAGG AACTTGCTCA GCGAGTGCGA AATGAAATAA CAAGCAACTA CAAGCAACAA 
ATTGGCAAGC TTTCTATGTC AGAGAAGTTC CACGACTGTA GAGGTCTTCA GTTCTTACTT 
ACAACACAAA TGGAAGAGCT AAATAAATTC CAGAAGCTAG TAAGAGAGGC TGTAAAAAAC 
CTGGAGGGAC CTCCATCTCG TAATGTTATT GAATCGGCGA CAATCTGCCA CCTTCGACCA 
ACCAGACTTC CTCTCAACTG CTGTGTCTTT TGTAAGGCTG ATGAATTGTT CACAGAATAT 
GAATCAAAGC TGTTTTCCCA CACAGTCAAA GGCCAGACTG CAATATTTGA GGAGATGATA 
GAGGATGAAG AAGGATTGGT GGATGATCGA ATACCCACCA CAAGCCGGGG TCTGTGGGCA 
ATAAGTGAAA CAGAGCGGTC TCTGAAAGCA TTGCTCTCAT TTGCAAAATC ACATAGATTT 
GATGTTGAAT TCATTGATGA AGGAAGTACT TCAATGGACC TTTTTGAAGC ATGGAAAAAG 
GAATATAAGT TGCTTCATGA ATATTGGATG GCTCTGAGGA ATCGCGTGTC TGCTATTGAT 
GAACTTGCAA TGGCTACAGA ACGACTAAGA GTACGTCATC CTAGGGAGCC AAAGCCCAAC 
CCACCTGTTC TTCATATCAT TGAACCACAT GAGGTAGAAC AAAATCGTAT AAAACTACTA 
AATGATAAAG CCGTTGCTAC TTCACAGCTT CAGAAAAAAC TTGGGCAGCT TCTTTACCTA 
ACTAACTTGG AAAAGTCTCA AGATAAAACA TCAGGAGGGA TTAATCCAGA ACCTTGTCCA 
ATCTGTGCTC GGCAGCTGGG AAAACAGTGG GCAGTACTGA CCTGTGGACA CTGTTTCTGT 
AATGAGTGCA TTTCAATTAT AATTGAACAG TACAGCGTGG GGTCTCACAG AAGTTCCATT 
AAGTGTGCCA TCTGCCGCCA GACCACATCT CACAAAGAAA TCTCATATGT CTTCACCTCA 
GAGAAAGCCA GCCAGGAAGA AGACATCCCT GTGAAGGGCA GCCATTCTAC AAAAGTGGAA 
GCTGTGGTCA GAACTCTGAT GAGAATCCAA CTTAGAGACC CAGGGGCTAA AGCACTCGTC 
TTCTCAACGT GGCAAGATGT ATTAGATATT ATTTCAAAAG CCCTCACTGA CAACAACATG 
GAATTTGCTC AAATCAGTCG TGTTAAGACA TTTCAGGAGA ACCTTTCAGC ATTTAAACAT 
GATCCCCAAA TCAATATCTT GCTGCTGCCC CTGCACACAG GTTCTAATGG ATTAACTATC 
ATTGAAGCAA CTCATGTTCT CTTGGTGGAG CCCATCTTGA ACCCTGCCCA TGAGCTTCAG 
GCCATTGGGA GGGTGCACCG GATTGGACAA ACAAAACCTA CTATTGTACA CAGATTCTTA 
ATTAAAGCAA CAATAGAAGA AAGAATGCAG GCAATGCTGA AAACTGCTGA GAAGAGTCAC 
ACGAGCTCAT CGGTGAAGCA TCCCGAAGCT TCCGTCTTAA CCTTGGCTGA TCTGGCGGAT 
CTGTTTACCA AAGAAACTGA AGAACTTGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHPRH ( XM_005615488.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005615545.1 Canis lupus familiaris SNF2 histone linker PHD RING helicase (SHPRH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe00919
Clone ID Related Accession (Same CDS sequence)	XM_005615488.3 , XM_005615487.2 , XM_005615488.2 , XM_005615487.3 , XM_533438.5 , XM_533438.4
Accession Version	XM_005615488.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase SHPRH
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005615545.1
CDS	144..5201
Translation	MSSRRKRAPPVRVDEEKQQQLHWNMHEDRRNEPLTLTDDENPCSGSDFSSAHCIILGDSLKEEVAHKDKKRCSEAVSISKSNDKEETCGTFSPLSIKLNIVISPYHFDNSWKAFLGELTLQLLPEQSLIEHFSEKSFTLMSSESSDHFLIYVNSECEDVEKQEKGLNEPISTCDKGIRVESSFSGEILEDLGWLQKKRRIKLYQKPEGNHMIKVGIYLLEGGLAKLDFLSDANSRMKKFNQLMKRVMEKLYNFVIPDVLEEDEEESESEPEGQEIDELYQFVKQTHQQETQSIQVDVQHPALIPVLRPYQREAVNWMLQQEHFRSAPTNENALHFLWREIVTPEDLKLYYNPYTGCIIRDYPNAGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPSHYSGGKVKNTETQNMEFEPKEKVQCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIYRYDVQRNRSLLKRMLKCLIFEGLVKQIKGHGFSGTFTLGKNYKEEDIYDKTKKQAVGSPRKIQKESRKSGSKDTDSEYLPSNTSDDDDDPYYYYYKARRNRSKFRKKPVTSTKKGKGQPNINLSSQDHCPATNDSGITDVATSKSACIFEGKQEHAAEDQAESLNPADGDVPQSTILSPCNSSDYRFECICGELDQVDRKPRVQCLKCHLWQHAKCVNYEEKNLKIKPFYCPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKDGFLQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPAVKAAEMAQRLSGINRWCISGTPVQRGLEDLFGLVVFLGIEPYCVKHWWVRLLYRPYCKKNPQHLYSFIAKILWRSAKKDVIDQIQIPPQTEEIHWLHFSPVERHFYHRQHEVCCQDAVVKLRKISDWALKLSSLDRRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLVAKHPGIPPTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQTIKELQRKIYSNSPWWLNVIHRAIEFAIDEELAQRVRNEITSNYKQQIGKLSMSEKFHDCRGLQFLLTTQMEELNKFQKLVREAVKNLEGPPSRNVIESATICHLRPTRLPLNCCVFCKADELFTEYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRIPTTSRGLWAISETERSLKALLSFAKSHRFDVEFIDEGSTSMDLFEAWKKEYKLLHEYWMALRNRVSAIDELAMATERLRVRHPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSGGINPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSEKASQEEDIPVKGSHSTKVEAVVRTLMRIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKHDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAEKSHTSSSVKHPEASVLTLADLADLFTKETEELE

Target ORF information:

RefSeq Version	XM_005615488.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SNF2 histone linker PHD RING helicase (SHPRH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615488.3

ORF Insert Sequence:

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHPRH ( XM_005615487.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005615544.1 Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X1, mRNA.

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CloneID	OCe00919
Clone ID Related Accession (Same CDS sequence)	XM_005615488.3 , XM_005615487.2 , XM_005615488.2 , XM_005615487.3 , XM_533438.5 , XM_533438.4
Accession Version	XM_005615487.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase SHPRH isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005615544.1
CDS	240..5297
Translation	MSSRRKRAPPVRVDEEKQQQLHWNMHEDRRNEPLTLTDDENPCSGSDFSSAHCIILGDSLKEEVAHKDKKRCSEAVSISKSNDKEETCGTFSPLSIKLNIVISPYHFDNSWKAFLGELTLQLLPEQSLIEHFSEKSFTLMSSESSDHFLIYVNSECEDVEKQEKGLNEPISTCDKGIRVESSFSGEILEDLGWLQKKRRIKLYQKPEGNHMIKVGIYLLEGGLAKLDFLSDANSRMKKFNQLMKRVMEKLYNFVIPDVLEEDEEESESEPEGQEIDELYQFVKQTHQQETQSIQVDVQHPALIPVLRPYQREAVNWMLQQEHFRSAPTNENALHFLWREIVTPEDLKLYYNPYTGCIIRDYPNAGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPSHYSGGKVKNTETQNMEFEPKEKVQCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIYRYDVQRNRSLLKRMLKCLIFEGLVKQIKGHGFSGTFTLGKNYKEEDIYDKTKKQAVGSPRKIQKESRKSGSKDTDSEYLPSNTSDDDDDPYYYYYKARRNRSKFRKKPVTSTKKGKGQPNINLSSQDHCPATNDSGITDVATSKSACIFEGKQEHAAEDQAESLNPADGDVPQSTILSPCNSSDYRFECICGELDQVDRKPRVQCLKCHLWQHAKCVNYEEKNLKIKPFYCPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKDGFLQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPAVKAAEMAQRLSGINRWCISGTPVQRGLEDLFGLVVFLGIEPYCVKHWWVRLLYRPYCKKNPQHLYSFIAKILWRSAKKDVIDQIQIPPQTEEIHWLHFSPVERHFYHRQHEVCCQDAVVKLRKISDWALKLSSLDRRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLVAKHPGIPPTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQTIKELQRKIYSNSPWWLNVIHRAIEFAIDEELAQRVRNEITSNYKQQIGKLSMSEKFHDCRGLQFLLTTQMEELNKFQKLVREAVKNLEGPPSRNVIESATICHLRPTRLPLNCCVFCKADELFTEYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRIPTTSRGLWAISETERSLKALLSFAKSHRFDVEFIDEGSTSMDLFEAWKKEYKLLHEYWMALRNRVSAIDELAMATERLRVRHPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSGGINPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSEKASQEEDIPVKGSHSTKVEAVVRTLMRIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKHDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAEKSHTSSSVKHPEASVLTLADLADLFTKETEELE

Target ORF information:

RefSeq Version	XM_005615487.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615487.2

ORF Insert Sequence:

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHPRH ( XM_005615488.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005615545.1 Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X3, mRNA.

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CloneID	OCe00919
Clone ID Related Accession (Same CDS sequence)	XM_005615488.3 , XM_005615487.2 , XM_005615488.2 , XM_005615487.3 , XM_533438.5 , XM_533438.4
Accession Version	XM_005615488.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase SHPRH isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005615545.1
CDS	191..5248
Translation	MSSRRKRAPPVRVDEEKQQQLHWNMHEDRRNEPLTLTDDENPCSGSDFSSAHCIILGDSLKEEVAHKDKKRCSEAVSISKSNDKEETCGTFSPLSIKLNIVISPYHFDNSWKAFLGELTLQLLPEQSLIEHFSEKSFTLMSSESSDHFLIYVNSECEDVEKQEKGLNEPISTCDKGIRVESSFSGEILEDLGWLQKKRRIKLYQKPEGNHMIKVGIYLLEGGLAKLDFLSDANSRMKKFNQLMKRVMEKLYNFVIPDVLEEDEEESESEPEGQEIDELYQFVKQTHQQETQSIQVDVQHPALIPVLRPYQREAVNWMLQQEHFRSAPTNENALHFLWREIVTPEDLKLYYNPYTGCIIRDYPNAGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPSHYSGGKVKNTETQNMEFEPKEKVQCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIYRYDVQRNRSLLKRMLKCLIFEGLVKQIKGHGFSGTFTLGKNYKEEDIYDKTKKQAVGSPRKIQKESRKSGSKDTDSEYLPSNTSDDDDDPYYYYYKARRNRSKFRKKPVTSTKKGKGQPNINLSSQDHCPATNDSGITDVATSKSACIFEGKQEHAAEDQAESLNPADGDVPQSTILSPCNSSDYRFECICGELDQVDRKPRVQCLKCHLWQHAKCVNYEEKNLKIKPFYCPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKDGFLQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPAVKAAEMAQRLSGINRWCISGTPVQRGLEDLFGLVVFLGIEPYCVKHWWVRLLYRPYCKKNPQHLYSFIAKILWRSAKKDVIDQIQIPPQTEEIHWLHFSPVERHFYHRQHEVCCQDAVVKLRKISDWALKLSSLDRRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLVAKHPGIPPTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQTIKELQRKIYSNSPWWLNVIHRAIEFAIDEELAQRVRNEITSNYKQQIGKLSMSEKFHDCRGLQFLLTTQMEELNKFQKLVREAVKNLEGPPSRNVIESATICHLRPTRLPLNCCVFCKADELFTEYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRIPTTSRGLWAISETERSLKALLSFAKSHRFDVEFIDEGSTSMDLFEAWKKEYKLLHEYWMALRNRVSAIDELAMATERLRVRHPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSGGINPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSEKASQEEDIPVKGSHSTKVEAVVRTLMRIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKHDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAEKSHTSSSVKHPEASVLTLADLADLFTKETEELE

Target ORF information:

RefSeq Version	XM_005615488.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615488.2

ORF Insert Sequence:

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHPRH ( XM_005615487.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005615544.1 Canis lupus familiaris SNF2 histone linker PHD RING helicase (SHPRH), transcript variant X1, mRNA.

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CloneID	OCe00919
Clone ID Related Accession (Same CDS sequence)	XM_005615488.3 , XM_005615487.2 , XM_005615488.2 , XM_005615487.3 , XM_533438.5 , XM_533438.4
Accession Version	XM_005615487.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase SHPRH
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005615544.1
CDS	219..5276
Translation	MSSRRKRAPPVRVDEEKQQQLHWNMHEDRRNEPLTLTDDENPCSGSDFSSAHCIILGDSLKEEVAHKDKKRCSEAVSISKSNDKEETCGTFSPLSIKLNIVISPYHFDNSWKAFLGELTLQLLPEQSLIEHFSEKSFTLMSSESSDHFLIYVNSECEDVEKQEKGLNEPISTCDKGIRVESSFSGEILEDLGWLQKKRRIKLYQKPEGNHMIKVGIYLLEGGLAKLDFLSDANSRMKKFNQLMKRVMEKLYNFVIPDVLEEDEEESESEPEGQEIDELYQFVKQTHQQETQSIQVDVQHPALIPVLRPYQREAVNWMLQQEHFRSAPTNENALHFLWREIVTPEDLKLYYNPYTGCIIRDYPNAGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPSHYSGGKVKNTETQNMEFEPKEKVQCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIYRYDVQRNRSLLKRMLKCLIFEGLVKQIKGHGFSGTFTLGKNYKEEDIYDKTKKQAVGSPRKIQKESRKSGSKDTDSEYLPSNTSDDDDDPYYYYYKARRNRSKFRKKPVTSTKKGKGQPNINLSSQDHCPATNDSGITDVATSKSACIFEGKQEHAAEDQAESLNPADGDVPQSTILSPCNSSDYRFECICGELDQVDRKPRVQCLKCHLWQHAKCVNYEEKNLKIKPFYCPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKDGFLQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPAVKAAEMAQRLSGINRWCISGTPVQRGLEDLFGLVVFLGIEPYCVKHWWVRLLYRPYCKKNPQHLYSFIAKILWRSAKKDVIDQIQIPPQTEEIHWLHFSPVERHFYHRQHEVCCQDAVVKLRKISDWALKLSSLDRRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLVAKHPGIPPTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQTIKELQRKIYSNSPWWLNVIHRAIEFAIDEELAQRVRNEITSNYKQQIGKLSMSEKFHDCRGLQFLLTTQMEELNKFQKLVREAVKNLEGPPSRNVIESATICHLRPTRLPLNCCVFCKADELFTEYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRIPTTSRGLWAISETERSLKALLSFAKSHRFDVEFIDEGSTSMDLFEAWKKEYKLLHEYWMALRNRVSAIDELAMATERLRVRHPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSGGINPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSEKASQEEDIPVKGSHSTKVEAVVRTLMRIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKHDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAEKSHTSSSVKHPEASVLTLADLADLFTKETEELE

Target ORF information:

RefSeq Version	XM_005615487.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SNF2 histone linker PHD RING helicase (SHPRH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005615487.3

ORF Insert Sequence:

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHPRH ( XM_533438.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_533438.2 Canis lupus familiaris SNF2 histone linker PHD RING helicase (SHPRH), transcript variant X2, mRNA.

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CloneID	OCe00919
Clone ID Related Accession (Same CDS sequence)	XM_005615488.3 , XM_005615487.2 , XM_005615488.2 , XM_005615487.3 , XM_533438.5 , XM_533438.4
Accession Version	XM_533438.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase SHPRH
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_533438.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533438.2
CDS	213..5270
Translation	MSSRRKRAPPVRVDEEKQQQLHWNMHEDRRNEPLTLTDDENPCSGSDFSSAHCIILGDSLKEEVAHKDKKRCSEAVSISKSNDKEETCGTFSPLSIKLNIVISPYHFDNSWKAFLGELTLQLLPEQSLIEHFSEKSFTLMSSESSDHFLIYVNSECEDVEKQEKGLNEPISTCDKGIRVESSFSGEILEDLGWLQKKRRIKLYQKPEGNHMIKVGIYLLEGGLAKLDFLSDANSRMKKFNQLMKRVMEKLYNFVIPDVLEEDEEESESEPEGQEIDELYQFVKQTHQQETQSIQVDVQHPALIPVLRPYQREAVNWMLQQEHFRSAPTNENALHFLWREIVTPEDLKLYYNPYTGCIIRDYPNAGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPSHYSGGKVKNTETQNMEFEPKEKVQCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIYRYDVQRNRSLLKRMLKCLIFEGLVKQIKGHGFSGTFTLGKNYKEEDIYDKTKKQAVGSPRKIQKESRKSGSKDTDSEYLPSNTSDDDDDPYYYYYKARRNRSKFRKKPVTSTKKGKGQPNINLSSQDHCPATNDSGITDVATSKSACIFEGKQEHAAEDQAESLNPADGDVPQSTILSPCNSSDYRFECICGELDQVDRKPRVQCLKCHLWQHAKCVNYEEKNLKIKPFYCPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKDGFLQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPAVKAAEMAQRLSGINRWCISGTPVQRGLEDLFGLVVFLGIEPYCVKHWWVRLLYRPYCKKNPQHLYSFIAKILWRSAKKDVIDQIQIPPQTEEIHWLHFSPVERHFYHRQHEVCCQDAVVKLRKISDWALKLSSLDRRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLVAKHPGIPPTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQTIKELQRKIYSNSPWWLNVIHRAIEFAIDEELAQRVRNEITSNYKQQIGKLSMSEKFHDCRGLQFLLTTQMEELNKFQKLVREAVKNLEGPPSRNVIESATICHLRPTRLPLNCCVFCKADELFTEYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRIPTTSRGLWAISETERSLKALLSFAKSHRFDVEFIDEGSTSMDLFEAWKKEYKLLHEYWMALRNRVSAIDELAMATERLRVRHPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSGGINPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSEKASQEEDIPVKGSHSTKVEAVVRTLMRIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKHDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAEKSHTSSSVKHPEASVLTLADLADLFTKETEELE

Target ORF information:

RefSeq Version	XM_533438.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SNF2 histone linker PHD RING helicase (SHPRH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533438.5

ORF Insert Sequence:

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHPRH ( XM_533438.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_533438.2 Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X2, mRNA.

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CloneID	OCe00919
Clone ID Related Accession (Same CDS sequence)	XM_005615488.3 , XM_005615487.2 , XM_005615488.2 , XM_005615487.3 , XM_533438.5 , XM_533438.4
Accession Version	XM_533438.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase SHPRH isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_533438.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_533438.2
CDS	211..5268
Translation	MSSRRKRAPPVRVDEEKQQQLHWNMHEDRRNEPLTLTDDENPCSGSDFSSAHCIILGDSLKEEVAHKDKKRCSEAVSISKSNDKEETCGTFSPLSIKLNIVISPYHFDNSWKAFLGELTLQLLPEQSLIEHFSEKSFTLMSSESSDHFLIYVNSECEDVEKQEKGLNEPISTCDKGIRVESSFSGEILEDLGWLQKKRRIKLYQKPEGNHMIKVGIYLLEGGLAKLDFLSDANSRMKKFNQLMKRVMEKLYNFVIPDVLEEDEEESESEPEGQEIDELYQFVKQTHQQETQSIQVDVQHPALIPVLRPYQREAVNWMLQQEHFRSAPTNENALHFLWREIVTPEDLKLYYNPYTGCIIRDYPNAGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPSHYSGGKVKNTETQNMEFEPKEKVQCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIYRYDVQRNRSLLKRMLKCLIFEGLVKQIKGHGFSGTFTLGKNYKEEDIYDKTKKQAVGSPRKIQKESRKSGSKDTDSEYLPSNTSDDDDDPYYYYYKARRNRSKFRKKPVTSTKKGKGQPNINLSSQDHCPATNDSGITDVATSKSACIFEGKQEHAAEDQAESLNPADGDVPQSTILSPCNSSDYRFECICGELDQVDRKPRVQCLKCHLWQHAKCVNYEEKNLKIKPFYCPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKDGFLQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPAVKAAEMAQRLSGINRWCISGTPVQRGLEDLFGLVVFLGIEPYCVKHWWVRLLYRPYCKKNPQHLYSFIAKILWRSAKKDVIDQIQIPPQTEEIHWLHFSPVERHFYHRQHEVCCQDAVVKLRKISDWALKLSSLDRRTVTSILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLVAKHPGIPPTLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALQPVQQTIKELQRKIYSNSPWWLNVIHRAIEFAIDEELAQRVRNEITSNYKQQIGKLSMSEKFHDCRGLQFLLTTQMEELNKFQKLVREAVKNLEGPPSRNVIESATICHLRPTRLPLNCCVFCKADELFTEYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRIPTTSRGLWAISETERSLKALLSFAKSHRFDVEFIDEGSTSMDLFEAWKKEYKLLHEYWMALRNRVSAIDELAMATERLRVRHPREPKPNPPVLHIIEPHEVEQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSGGINPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSEKASQEEDIPVKGSHSTKVEAVVRTLMRIQLRDPGAKALVFSTWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKHDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAEKSHTSSSVKHPEASVLTLADLADLFTKETEELE

Target ORF information:

RefSeq Version	XM_533438.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase (SHPRH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_533438.4

ORF Insert Sequence:

ATGAGTAGCC GGAGGAAACG TGCTCCTCCA GTGAGAGTTG ATGAGGAAAA ACAACAGCAG 
CTTCATTGGA ATATGCATGA AGACAGAAGA AATGAACCTC TCACCCTAAC TGATGATGAG 
AACCCCTGCT CTGGTTCAGA TTTCTCTTCT GCTCACTGCA TCATCCTAGG TGATAGTCTA 
AAGGAAGAAG TGGCTCACAA AGATAAAAAG AGGTGTTCGG AGGCAGTGAG CATCTCAAAA 
TCAAACGATA AAGAAGAGAC TTGTGGCACT TTTTCTCCTT TGTCTATAAA GCTGAATATC 
GTGATTTCTC CCTATCACTT CGATAATTCT TGGAAGGCAT TTCTAGGAGA ATTAACTCTT 
CAGCTTCTTC CTGAACAGAG TTTAATTGAA CATTTTTCTG AAAAGAGTTT TACACTGATG 
AGTTCAGAGT CAAGCGATCA TTTCCTGATC TATGTTAATT CAGAATGTGA AGATGTAGAG 
AAGCAAGAAA AAGGACTCAA TGAGCCAATT AGTACTTGTG ACAAGGGTAT TCGAGTGGAA 
TCATCTTTCA GTGGTGAAAT ATTAGAAGAT TTGGGGTGGC TACAAAAGAA GAGAAGAATA 
AAACTTTACC AAAAACCAGA AGGAAATCAC ATGATCAAGG TTGGAATTTA TCTTTTGGAA 
GGTGGTCTAG CGAAACTGGA CTTTTTGAGT GATGCAAATT CAAGAATGAA GAAATTCAAT 
CAACTTATGA AAAGAGTGAT GGAAAAGTTA TACAATTTTG TTATTCCAGA TGTGTTGGAA 
GAGGATGAGG AAGAGTCAGA GAGTGAGCCA GAGGGACAGG AGATTGATGA ACTCTATCAG 
TTTGTGAAGC AAACACATCA GCAAGAAACA CAGTCCATCC AAGTGGATGT CCAGCATCCT 
GCCTTGATTC CTGTGTTGAG ACCATACCAG AGAGAGGCTG TCAATTGGAT GCTACAACAA 
GAGCATTTCA GAAGTGCTCC TACTAATGAA AATGCCTTGC ACTTCTTATG GCGTGAGATT 
GTTACACCTG AGGATTTGAA ACTCTACTAC AATCCATATA CAGGCTGCAT CATCCGTGAT 
TACCCAAATG CTGGGCCACA GTTGCTTGGT GGAATCTTAG CAGATGAGAT GGGTCTCGGA 
AAGACAGTGG AGGTTTTGGC TCTGATTCTG ACACATACTC GACAAGATGT CAAACAAGAT 
GCTCTCACTC TTCCTGAGGG AAAAGTGGTG AATTATTTCA TTCCATCACA TTATTCTGGA 
GGAAAAGTAA AAAACACAGA AACCCAGAAT ATGGAATTTG AACCAAAAGA AAAAGTTCAG 
TGCCCCCCTA CACGTGTGAT GATCCTGACA GCTGTGAAAG AAATGAATGG AAAAAAAGGA 
GTTTCCATCC TTTCCATCTA TAAATATGTC AGTTCTATAT ATAGATATGA TGTTCAGCGG 
AACAGGAGTC TTCTGAAACG GATGCTGAAA TGTTTAATTT TTGAAGGTCT TGTGAAACAG 
ATCAAAGGCC ATGGTTTTTC TGGAACTTTT ACATTGGGGA AGAATTACAA GGAAGAGGAT 
ATATATGATA AAACAAAAAA GCAGGCAGTA GGGAGTCCAA GGAAAATTCA GAAAGAATCC 
AGAAAATCAG GGAGCAAAGA CACAGATTCT GAATACTTGC CATCAAACAC ATCTGATGAT 
GATGATGATC CTTACTATTA TTATTATAAG GCTCGGAGAA ATCGTAGTAA ATTCAGGAAA 
AAGCCTGTTA CATCCACAAA AAAGGGAAAA GGTCAGCCTA ATATCAATCT CAGTTCACAA 
GATCACTGTC CAGCTACCAA TGACTCTGGA ATTACCGATG TTGCCACATC TAAAAGTGCA 
TGTATCTTTG AAGGCAAGCA AGAACATGCA GCTGAGGACC AAGCTGAATC TCTAAACCCT 
GCTGATGGTG ATGTGCCACA ATCTACTATC TTGAGTCCCT GTAATTCCTC TGATTATCGC 
TTTGAATGCA TATGTGGTGA ACTTGACCAA GTAGACCGTA AACCTCGTGT TCAGTGCCTG 
AAATGCCATC TGTGGCAACA TGCAAAATGT GTGAATTATG AAGAGAAGAA TCTGAAGATT 
AAACCTTTTT ACTGTCCCCA CTGTCTTGTT GCAATGGAGC CAGTATCAAC AAGAGCAACT 
CTGATCATCT CGCCCAGTTC CATCTGTCAT CAGTGGGTGG ATGAAATCAA CAGGCATGTG 
AGGTCATCAT CTCTTCGAGT CTTGGTATAC CAAGGAGTGA AGAAAGATGG TTTTTTACAA 
CCTCATTTTT TGGCTGAACA GGATATAGTT ATCATTACCT ATGATGTCCT TCGTTCAGAA 
CTAAACTATG TAGATATCCC ACACAGCAAT AGTGAGGATG GACGTCGCTT ACGGAACCAG 
AAGCGCTATA TGGCCATTCC CAGCCCCCTA GTAGCTGTGG AGTGGTGGAG GATCTGCCTT 
GATGAAGCCC AGATGGTTGA ATGTCCTGCT GTGAAAGCTG CAGAAATGGC TCAGCGCTTG 
AGTGGGATTA ATCGCTGGTG CATCAGTGGC ACTCCAGTGC AAAGAGGATT GGAGGATCTT 
TTTGGGTTGG TGGTCTTTCT TGGTATTGAA CCTTACTGTG TCAAACACTG GTGGGTTCGA 
CTTCTCTATC GCCCTTACTG CAAGAAGAAT CCTCAGCATC TCTACAGCTT TATTGCCAAG 
ATACTATGGA GGTCTGCAAA GAAGGATGTG ATTGACCAAA TCCAGATACC CCCTCAGACA 
GAAGAAATCC ACTGGCTTCA CTTTTCTCCA GTGGAAAGAC ATTTCTATCA CCGTCAGCAT 
GAAGTGTGCT GCCAGGATGC TGTAGTAAAA CTCAGGAAGA TTTCAGACTG GGCGCTGAAG 
CTCAGCAGCC TGGACAGAAG GACTGTCACC TCTATCCTAT ATCCATTGCT GAGACTCAGG 
CAGGCCTGCT GTCATCCACA GGCTGTTCGT GGGGAGTTCT TGCCACTCCA GAAAAGCACC 
ATGACAATGG AAGAGCTGCT GACATCTCTG CAGAAGAAAT GTGGAACTGA ATGTGAAGAA 
GCACATCGGC AACTAGTTTG TGCTCTCAAT GGCTTAGCCG GCATCCACAT CATTAAAGGT 
GAGTATGCCT TGGCAGCAGA ACTGTACAGA GAAGTATTGC GTTCATCTGA GGAACACAAA 
GGAAAACTCA AAACAGATTC ACTCCAAAGA CTACATGCTA CCCATAACTT GATGGAATTG 
TTGGTAGCCA AGCACCCAGG AATACCTCCT ACCTTACGAG ATGGCAGACT TGAGGAAGAG 
GCTAAACAAC TGCGAGAGCA TTATATGAGC AAGTGTAATA CAGAAGTCGC TGAAGCTCAG 
CAAGCTTTAC AGCCCGTGCA GCAGACCATA AAGGAGCTCC AGAGAAAGAT TTATTCTAAT 
TCTCCTTGGT GGCTGAATGT GATCCACAGA GCAATAGAAT TTGCTATTGA TGAGGAACTT 
GCTCAGCGAG TGCGAAATGA AATAACAAGC AACTACAAGC AACAAATTGG CAAGCTTTCT 
ATGTCAGAGA AGTTCCACGA CTGTAGAGGT CTTCAGTTCT TACTTACAAC ACAAATGGAA 
GAGCTAAATA AATTCCAGAA GCTAGTAAGA GAGGCTGTAA AAAACCTGGA GGGACCTCCA 
TCTCGTAATG TTATTGAATC GGCGACAATC TGCCACCTTC GACCAACCAG ACTTCCTCTC 
AACTGCTGTG TCTTTTGTAA GGCTGATGAA TTGTTCACAG AATATGAATC AAAGCTGTTT 
TCCCACACAG TCAAAGGCCA GACTGCAATA TTTGAGGAGA TGATAGAGGA TGAAGAAGGA 
TTGGTGGATG ATCGAATACC CACCACAAGC CGGGGTCTGT GGGCAATAAG TGAAACAGAG 
CGGTCTCTGA AAGCATTGCT CTCATTTGCA AAATCACATA GATTTGATGT TGAATTCATT 
GATGAAGGAA GTACTTCAAT GGACCTTTTT GAAGCATGGA AAAAGGAATA TAAGTTGCTT 
CATGAATATT GGATGGCTCT GAGGAATCGC GTGTCTGCTA TTGATGAACT TGCAATGGCT 
ACAGAACGAC TAAGAGTACG TCATCCTAGG GAGCCAAAGC CCAACCCACC TGTTCTTCAT 
ATCATTGAAC CACATGAGGT AGAACAAAAT CGTATAAAAC TACTAAATGA TAAAGCCGTT 
GCTACTTCAC AGCTTCAGAA AAAACTTGGG CAGCTTCTTT ACCTAACTAA CTTGGAAAAG 
TCTCAAGATA AAACATCAGG AGGGATTAAT CCAGAACCTT GTCCAATCTG TGCTCGGCAG 
CTGGGAAAAC AGTGGGCAGT ACTGACCTGT GGACACTGTT TCTGTAATGA GTGCATTTCA 
ATTATAATTG AACAGTACAG CGTGGGGTCT CACAGAAGTT CCATTAAGTG TGCCATCTGC 
CGCCAGACCA CATCTCACAA AGAAATCTCA TATGTCTTCA CCTCAGAGAA AGCCAGCCAG 
GAAGAAGACA TCCCTGTGAA GGGCAGCCAT TCTACAAAAG TGGAAGCTGT GGTCAGAACT 
CTGATGAGAA TCCAACTTAG AGACCCAGGG GCTAAAGCAC TCGTCTTCTC AACGTGGCAA 
GATGTATTAG ATATTATTTC AAAAGCCCTC ACTGACAACA ACATGGAATT TGCTCAAATC 
AGTCGTGTTA AGACATTTCA GGAGAACCTT TCAGCATTTA AACATGATCC CCAAATCAAT 
ATCTTGCTGC TGCCCCTGCA CACAGGTTCT AATGGATTAA CTATCATTGA AGCAACTCAT 
GTTCTCTTGG TGGAGCCCAT CTTGAACCCT GCCCATGAGC TTCAGGCCAT TGGGAGGGTG 
CACCGGATTG GACAAACAAA ACCTACTATT GTACACAGAT TCTTAATTAA AGCAACAATA 
GAAGAAAGAA TGCAGGCAAT GCTGAAAACT GCTGAGAAGA GTCACACGAG CTCATCGGTG 
AAGCATCCCG AAGCTTCCGT CTTAACCTTG GCTGATCTGG CGGATCTGTT TACCAAAGAA 
ACTGAAGAAC TTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SHPRH cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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