shprh cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	shprh
Entrez Gene ID	560526
Full Name	SNF2 histone linker PHD RING helicase
General protein information	Preferred Names SNF2 histone linker PHD RING helicase Names E3 ubiquitin-protein ligase SHPRH
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009292830.3	XP_009291105.1	E3 ubiquitin-protein ligase SHPRH isoform X2
XM_009292830.2	XP_009291105.1	E3 ubiquitin-protein ligase SHPRH isoform X2
XM_003200310.4	XP_003200358.1	E3 ubiquitin-protein ligase SHPRH isoform X1
XM_021473076.1	XP_021328751.1	E3 ubiquitin-protein ligase SHPRH isoform X4
XM_003200310.5	XP_003200358.1	E3 ubiquitin-protein ligase SHPRH isoform X1
XM_021473075.1	XP_021328750.1	E3 ubiquitin-protein ligase SHPRH isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SHPRH	XP_001172976.2
Bos taurus (cattle)	SHPRH	XP_002690346.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007417	XP_308421.4
Homo sapiens (human)	SHPRH	NP_001036148.2
Mus musculus (house mouse)	Shprh	NP_001071175.1
Gallus gallus (chicken)	SHPRH	XP_419651.3
	SHPRH	XP_001086512.1
Danio rerio (zebrafish)	shprh	XP_003200358.1
Drosophila melanogaster (fruit fly)	CG7376	NP_648034.1
Canis lupus familiaris (dog)	SHPRH	XP_533438.2
Rattus norvegicus (Norway rat)	Shprh	NP_001100940.1
Xenopus tropicalis (tropical clawed frog)	shprh	XP_004914950.1

Gene Ontology

The following shprh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the shprh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145841	XM_021473075.1 Latest version!	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa145842	XM_021473076.1 Latest version!	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa22222	XM_003200310.5 Latest version!	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa43299	XM_009292830.3 Latest version!	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa43299	XM_009292830.2	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa22222	XM_003200310.4	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

shprh ( XM_021473075.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328750.1 Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X3, mRNA.

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CloneID	ODa145841
Clone ID Related Accession (Same CDS sequence)	XM_021473075.1
Accession Version	XM_021473075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase SHPRH isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394976.1) and transcript sequence (GDQH01006930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2232 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGCAGCA GGAGGAAACG TGCACCGCCA GTGCGCGTGG ATGAGGATGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGATGA CATTTTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATAGA ACTGAACGTA ATGCCAATAT CTGCTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGAGA TGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAATC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCGTCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTAAG 
TCTGCTGACC AGCCCGCTCC CAGGAGAACA TCTCGTCGAG TTTCTGCTCC GGATAAAGAT 
TCCTCACTGA CCTCCTATAC AACAACAGCG TCAGATTCAG CATCGGAAGA CAGTATGAAT 
CCTCCTCTTC TCACAGAAGA GCTGAAATCT GATCCTGATT CACTTCTGGA CCCAGAAAAT 
CCCCTGCAGA AAACACCTGT AGACATAACA GATGATAAAG AAACACCCGA CAAACCCCAA 
CTGCAAATAA CAGAAGACGT CCATATTGAA TATAACAAAC CAGATCTTAC GCAAACCTCA 
AAGCCTGAAG TCGAAACGCC AACCAGAGCG TCTGTGGTCC CGTTTAATAC TCCAGACTAC 
CGCTTTGAGT GCATCTGTGG AGAGCTGGGC CTGGTGGACT ATAAGCCCCG GGTTCAGTGT 
CTGAACTGTC TGTTATGGCA GCACGCCGAG TGTGTCAACT ATAAGGAGGA GAATCTGGAA 
ACGACGCCGT TTTATTGTCC TCACTGTCTG GTGGCCATGA CACCAGTGTC CACTGGAGCG 
ACTCTTATCA TCTCACCCAG CTCTATTTGT CATCAGTGGG TGGATGAAAT CAACCGGCAC 
ATTCACACCT CCTCCCTCAG AGTGCTGGTG TATCAGGGCG TGAAGAGACA CGGTTTTATC 
CAGCCTCACA TTCTGGCCGA GCAGGATGTG GTCATCACCA CTTATGACGT TTTGCGCACT 
GAACTCAATT ATGTGGATAT TCCTCACAGC AATAGTAAAG ATGGCCGTCG ATTCCGCAAC 
CAGAAGCGCT ACATGGCCAT ACCGAGCCCT CTAGTGGCTG TGGAGTGGTG GCGCGTTTGT 
CTAGATGAAG CTCAGATGGT GGAGTGCACC ACAGCCAAGG CTGCAGAAAT GGCTTTGCGT 
CTCACATCTG TTAATCGCTG GTGTGTGAGT GGAACACCTG TGCAGAGAGG TTTGGAGGAC 
CTGTATGGTC TTGTCCTGTT CTTGGGAGTT GATCCCTACT GGGTGAAGTA CTGGTGGGAT 
CAGTTACTCT ATCGCCCGTA CCGTCACGGC AATACAGCAC CGCTCTACAA CGTCATTGGG 
CAACTACTAT GGCGATCTGC CAAAAAAGAC GTCATTGATC AGATCCAAAT CCCCCCTCAA 
ACAGAGGAGA TCCATTGGCT TAACTTCTCC CCCGTGGAAA GACACTTTTA CCACCGTCAG 
CATGAGGTTT GCTCCCAAGA TGCTTTAGTG AAGCTGAGGA AGATCTCTGA TTGGTCACTG 
AAGCTGGGAA GTTTGGACCG TCGTACCGTC ACTACCATAT TATACCCACT TTTGCGTCTG 
CGCCAGGCCT GCTGCCACCC GCAGGCTGTC CGAGGAGAAT TTCTACCCCT CCAAAAAAGC 
ACAATGACAA TGGAGGAGTT GTTGAAGTCG CTGCAGAAGA AATGTCGTGT GGAGTGTGAA 
GAAGCGCACA GGCAGCTGGT CTGTGCTCTC AACGGTCTGG CTGGAATCCA CATCATTAGA 
AGTGAGTTTG TAGATGCAGC GGAGATGTAC AGGGAAGTGT TGCGATCATC TGAAGAACAC 
AAAGCAAGGC TGAAAACTGA TTCCCTTCAG AGGCTTCATG CAACCCATAA TCTAATGGAA 
CTTCTAAGTG CAAACCACCC TGGAATTCCT CCAACCCTGA GAGATGACAG TCTCAAAGAA 
GAGGCCGAGC AGCTGAAGCA GCATTACATG GCCAAAGTGA ACTCTGAGGT TTCTGAAGCA 
CATCAAAACC TACAGCCTGT TCTTCAGCAC ATTAAAGAAC AAAGACGCAA GGTAAACCTT 
CGCTCCCCCT GGTGGCTGGA TGTCATACAG CAGGCCATGC AGTACTCCAT GGATGATGAT 
CTTGTCAGCC GCATTCAAAA TGAGCTCACA TGCAGTTATA AACAACAAGC CAACAAGCTC 
TCAATGGCAG ACAAATTTCG AGACGCTCGG GGTCTTCAGT TTCTGCTCAG CACTCAAATG 
GATGATTTGA TGAAGTCACA GAAAACAGTC CAGGATGCTG TGAAGAAGCT GGAAGGGCCG 
CCGTCTCAAC AAGTCATCGA GGAAGCAACA CTTTGCCACC TGCGACCTGT TCGCCTGCCA 
CTGAACAACT GTGTGTTTTG CAAAGCAGAT GAACTTTTTA CAGACTACGA GTCTAAGCTT 
TTCTCGCACA CAGTAAAAGG GCAGACAGCC ATCTTTGAGG AGATGATTGA AGACGAGGAA 
GGGCTGGTAG ATGACAGGCT TCCCACCACC AGTAGAGGAT TATGGGCCGC CAGTGAGACC 
GAACGAGCTC TTAAAGCCAT CTTGTCCTTC GCTAAAGTCC GCCGCTTGGA GTCTGGCCTC 
ATCGAGGAGG GAAACTCCTT CATGGAGCTC TTCGAGTCCT GGAAGAAAGA ATACAAGCTT 
CTGCATGAGT ACTGGATGTC TCTGCGGGAT CATGTATCTG CTATAGATGA GCTGGGAATG 
GCCACCGAGA GGCTGCGTGT GCGCTTACCT GATGAGCCCA AACCCAAAGT CCTGCATATT 
ATTGAGCCTC ACGAGGTGGA GCAGAACAGG ATAAAGCTGC TCAATGACAG GGCAGTGGCC 
AAATCACAGC TGCAAAAAAA GCTTGGGCAG TTTCTCTATC TCACAAACCT GGAGAAGTCT 
CAGGATAAGT CCACTGGTGG TCTGAATCCA GAACCATGTC CAATCTGTGC TCGTCCGCTG 
GGTCAGGAGT GGGCCGTACT GACCTGTGGA CACTGTTTCT GTAACGAGTG CATCGCCATC 
ATTGTGGAGC AATACAGCAT CGGCAATCGA CGGAGAGCTA TAAAGTGTGC CATATGCCGA 
CAAACCACTT CACACGCAGA GATCTCATAT GTGTTCACCA CTCAAAACAA CCACCAGGGG 
CAGGACATCC CTGTGAAGGG TAGCCACTCC ACGAAGGTCG AGGCTGTGGT TCGAGTCCTA 
AAAAAAGTCC AGATGACCGA CCCGGGAGCC AAGTCTCTTG TGTTCTCCAC GTGGCAGGGC 
GTCCTGGACA TCATCGCCAA AGCCCTGTTT GACAACAACA TGGAGTTTGC ACAGATCAAC 
GGAATTCACA AGTTTCAGGA GAACCTGAGT GCTTTCAAAT ATGAGGAGAA GATCAACATC 
TTGTTGCTGC CACTGCACAC AGGCTCCAAT GGACTGAACA TCATTGAAGC AACACACGTG 
CTACTGGTGG AGCCCATCCT AAACCCCGCC CACGAGCTGC AGGCCATAGG AAGAGTGCAT 
CGGATTGGCC AGACCAAACC CACATTTGTT CATCGGTTTC TGATTAAGTC CACCATTGAG 
GAACGGATGC AAGCCATGCT GAAAACAGCA GAAAAGAGTC ACAGTTCCAC GTCTATGAAG 
CATTCGGAGG CCTCTGTGTT TACAGTGGCA GACCTGGCAG ATCTGTTCAC CGAGGACAGC 
GAGACTCTTG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328750.1
CDS	142..5076
Translation	MSSRRKRAPPVRVDEDAKRRLDWNMHEDRKNEGACDDILESVPAPSDSCLPVPVLEQGFISSAGTEEEPSEERPSCSTQDSPPDTIELNVMPISALDQGWNALIGEFNLHPKIPIEVADGSFCLQQTGDTLSMTLNTLEMEDEMGRSDPLCSVPVACSLGGLLLEDLDWLQKRKVIKLCHASGGVPVKLKVFLIESGLGRPEFLSEGNGRIKKASQLLQKLMELFYDFIIPEVMENEEEACDTDLERQNVEELYDHVRHLHQTEAAEQSFHVQHPALIPILRPYQSQAVNWMLRREKYKISSSPEQRLHFLWREMVTICGKKLFYNPYTGCLIREFPLAGVDWPGGILADEMGLGKTVEVLALILCNTRQNLEHGVLTLPVGRSVNYFVPPPPLERQRIIKHEPRPKEKIIYPAVREMLLTAIKEMKVGKGASINAIFAYLRTMYKYDTLKNRNHIKKILTKLISENIVEQIKGRGLAGSFKLGKNYKEKKKKPVTARTKSADQPAPRRTSRRVSAPDKDSSLTSYTTTASDSASEDSMNPPLLTEELKSDPDSLLDPENPLQKTPVDITDDKETPDKPQLQITEDVHIEYNKPDLTQTSKPEVETPTRASVVPFNTPDYRFECICGELGLVDYKPRVQCLNCLLWQHAECVNYKEENLETTPFYCPHCLVAMTPVSTGATLIISPSSICHQWVDEINRHIHTSSLRVLVYQGVKRHGFIQPHILAEQDVVITTYDVLRTELNYVDIPHSNSKDGRRFRNQKRYMAIPSPLVAVEWWRVCLDEAQMVECTTAKAAEMALRLTSVNRWCVSGTPVQRGLEDLYGLVLFLGVDPYWVKYWWDQLLYRPYRHGNTAPLYNVIGQLLWRSAKKDVIDQIQIPPQTEEIHWLNFSPVERHFYHRQHEVCSQDALVKLRKISDWSLKLGSLDRRTVTTILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLKSLQKKCRVECEEAHRQLVCALNGLAGIHIIRSEFVDAAEMYREVLRSSEEHKARLKTDSLQRLHATHNLMELLSANHPGIPPTLRDDSLKEEAEQLKQHYMAKVNSEVSEAHQNLQPVLQHIKEQRRKVNLRSPWWLDVIQQAMQYSMDDDLVSRIQNELTCSYKQQANKLSMADKFRDARGLQFLLSTQMDDLMKSQKTVQDAVKKLEGPPSQQVIEEATLCHLRPVRLPLNNCVFCKADELFTDYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRLPTTSRGLWAASETERALKAILSFAKVRRLESGLIEEGNSFMELFESWKKEYKLLHEYWMSLRDHVSAIDELGMATERLRVRLPDEPKPKVLHIIEPHEVEQNRIKLLNDRAVAKSQLQKKLGQFLYLTNLEKSQDKSTGGLNPEPCPICARPLGQEWAVLTCGHCFCNECIAIIVEQYSIGNRRRAIKCAICRQTTSHAEISYVFTTQNNHQGQDIPVKGSHSTKVEAVVRVLKKVQMTDPGAKSLVFSTWQGVLDIIAKALFDNNMEFAQINGIHKFQENLSAFKYEEKINILLLPLHTGSNGLNIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTFVHRFLIKSTIEERMQAMLKTAEKSHSSTSMKHSEASVFTVADLADLFTEDSETLE

Target ORF information:

RefSeq Version	XM_021473075.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473075.1

ORF Insert Sequence:

ATGAGCAGCA GGAGGAAACG TGCACCGCCA GTGCGCGTGG ATGAGGATGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGATGA CATTTTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATAGA ACTGAACGTA ATGCCAATAT CTGCTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGAGA TGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAATC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCGTCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTAAG 
TCTGCTGACC AGCCCGCTCC CAGGAGAACA TCTCGTCGAG TTTCTGCTCC GGATAAAGAT 
TCCTCACTGA CCTCCTATAC AACAACAGCG TCAGATTCAG CATCGGAAGA CAGTATGAAT 
CCTCCTCTTC TCACAGAAGA GCTGAAATCT GATCCTGATT CACTTCTGGA CCCAGAAAAT 
CCCCTGCAGA AAACACCTGT AGACATAACA GATGATAAAG AAACACCCGA CAAACCCCAA 
CTGCAAATAA CAGAAGACGT CCATATTGAA TATAACAAAC CAGATCTTAC GCAAACCTCA 
AAGCCTGAAG TCGAAACGCC AACCAGAGCG TCTGTGGTCC CGTTTAATAC TCCAGACTAC 
CGCTTTGAGT GCATCTGTGG AGAGCTGGGC CTGGTGGACT ATAAGCCCCG GGTTCAGTGT 
CTGAACTGTC TGTTATGGCA GCACGCCGAG TGTGTCAACT ATAAGGAGGA GAATCTGGAA 
ACGACGCCGT TTTATTGTCC TCACTGTCTG GTGGCCATGA CACCAGTGTC CACTGGAGCG 
ACTCTTATCA TCTCACCCAG CTCTATTTGT CATCAGTGGG TGGATGAAAT CAACCGGCAC 
ATTCACACCT CCTCCCTCAG AGTGCTGGTG TATCAGGGCG TGAAGAGACA CGGTTTTATC 
CAGCCTCACA TTCTGGCCGA GCAGGATGTG GTCATCACCA CTTATGACGT TTTGCGCACT 
GAACTCAATT ATGTGGATAT TCCTCACAGC AATAGTAAAG ATGGCCGTCG ATTCCGCAAC 
CAGAAGCGCT ACATGGCCAT ACCGAGCCCT CTAGTGGCTG TGGAGTGGTG GCGCGTTTGT 
CTAGATGAAG CTCAGATGGT GGAGTGCACC ACAGCCAAGG CTGCAGAAAT GGCTTTGCGT 
CTCACATCTG TTAATCGCTG GTGTGTGAGT GGAACACCTG TGCAGAGAGG TTTGGAGGAC 
CTGTATGGTC TTGTCCTGTT CTTGGGAGTT GATCCCTACT GGGTGAAGTA CTGGTGGGAT 
CAGTTACTCT ATCGCCCGTA CCGTCACGGC AATACAGCAC CGCTCTACAA CGTCATTGGG 
CAACTACTAT GGCGATCTGC CAAAAAAGAC GTCATTGATC AGATCCAAAT CCCCCCTCAA 
ACAGAGGAGA TCCATTGGCT TAACTTCTCC CCCGTGGAAA GACACTTTTA CCACCGTCAG 
CATGAGGTTT GCTCCCAAGA TGCTTTAGTG AAGCTGAGGA AGATCTCTGA TTGGTCACTG 
AAGCTGGGAA GTTTGGACCG TCGTACCGTC ACTACCATAT TATACCCACT TTTGCGTCTG 
CGCCAGGCCT GCTGCCACCC GCAGGCTGTC CGAGGAGAAT TTCTACCCCT CCAAAAAAGC 
ACAATGACAA TGGAGGAGTT GTTGAAGTCG CTGCAGAAGA AATGTCGTGT GGAGTGTGAA 
GAAGCGCACA GGCAGCTGGT CTGTGCTCTC AACGGTCTGG CTGGAATCCA CATCATTAGA 
AGTGAGTTTG TAGATGCAGC GGAGATGTAC AGGGAAGTGT TGCGATCATC TGAAGAACAC 
AAAGCAAGGC TGAAAACTGA TTCCCTTCAG AGGCTTCATG CAACCCATAA TCTAATGGAA 
CTTCTAAGTG CAAACCACCC TGGAATTCCT CCAACCCTGA GAGATGACAG TCTCAAAGAA 
GAGGCCGAGC AGCTGAAGCA GCATTACATG GCCAAAGTGA ACTCTGAGGT TTCTGAAGCA 
CATCAAAACC TACAGCCTGT TCTTCAGCAC ATTAAAGAAC AAAGACGCAA GGTAAACCTT 
CGCTCCCCCT GGTGGCTGGA TGTCATACAG CAGGCCATGC AGTACTCCAT GGATGATGAT 
CTTGTCAGCC GCATTCAAAA TGAGCTCACA TGCAGTTATA AACAACAAGC CAACAAGCTC 
TCAATGGCAG ACAAATTTCG AGACGCTCGG GGTCTTCAGT TTCTGCTCAG CACTCAAATG 
GATGATTTGA TGAAGTCACA GAAAACAGTC CAGGATGCTG TGAAGAAGCT GGAAGGGCCG 
CCGTCTCAAC AAGTCATCGA GGAAGCAACA CTTTGCCACC TGCGACCTGT TCGCCTGCCA 
CTGAACAACT GTGTGTTTTG CAAAGCAGAT GAACTTTTTA CAGACTACGA GTCTAAGCTT 
TTCTCGCACA CAGTAAAAGG GCAGACAGCC ATCTTTGAGG AGATGATTGA AGACGAGGAA 
GGGCTGGTAG ATGACAGGCT TCCCACCACC AGTAGAGGAT TATGGGCCGC CAGTGAGACC 
GAACGAGCTC TTAAAGCCAT CTTGTCCTTC GCTAAAGTCC GCCGCTTGGA GTCTGGCCTC 
ATCGAGGAGG GAAACTCCTT CATGGAGCTC TTCGAGTCCT GGAAGAAAGA ATACAAGCTT 
CTGCATGAGT ACTGGATGTC TCTGCGGGAT CATGTATCTG CTATAGATGA GCTGGGAATG 
GCCACCGAGA GGCTGCGTGT GCGCTTACCT GATGAGCCCA AACCCAAAGT CCTGCATATT 
ATTGAGCCTC ACGAGGTGGA GCAGAACAGG ATAAAGCTGC TCAATGACAG GGCAGTGGCC 
AAATCACAGC TGCAAAAAAA GCTTGGGCAG TTTCTCTATC TCACAAACCT GGAGAAGTCT 
CAGGATAAGT CCACTGGTGG TCTGAATCCA GAACCATGTC CAATCTGTGC TCGTCCGCTG 
GGTCAGGAGT GGGCCGTACT GACCTGTGGA CACTGTTTCT GTAACGAGTG CATCGCCATC 
ATTGTGGAGC AATACAGCAT CGGCAATCGA CGGAGAGCTA TAAAGTGTGC CATATGCCGA 
CAAACCACTT CACACGCAGA GATCTCATAT GTGTTCACCA CTCAAAACAA CCACCAGGGG 
CAGGACATCC CTGTGAAGGG TAGCCACTCC ACGAAGGTCG AGGCTGTGGT TCGAGTCCTA 
AAAAAAGTCC AGATGACCGA CCCGGGAGCC AAGTCTCTTG TGTTCTCCAC GTGGCAGGGC 
GTCCTGGACA TCATCGCCAA AGCCCTGTTT GACAACAACA TGGAGTTTGC ACAGATCAAC 
GGAATTCACA AGTTTCAGGA GAACCTGAGT GCTTTCAAAT ATGAGGAGAA GATCAACATC 
TTGTTGCTGC CACTGCACAC AGGCTCCAAT GGACTGAACA TCATTGAAGC AACACACGTG 
CTACTGGTGG AGCCCATCCT AAACCCCGCC CACGAGCTGC AGGCCATAGG AAGAGTGCAT 
CGGATTGGCC AGACCAAACC CACATTTGTT CATCGGTTTC TGATTAAGTC CACCATTGAG 
GAACGGATGC AAGCCATGCT GAAAACAGCA GAAAAGAGTC ACAGTTCCAC GTCTATGAAG 
CATTCGGAGG CCTCTGTGTT TACAGTGGCA GACCTGGCAG ATCTGTTCAC CGAGGACAGC 
GAGACTCTTG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shprh ( XM_021473076.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328751.1 Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X4, mRNA.

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CloneID	ODa145842
Clone ID Related Accession (Same CDS sequence)	XM_021473076.1
Accession Version	XM_021473076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase SHPRH isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394976.1) and transcript sequence (GDQH01006930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 2232 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGCAGCA GGAGGAAACG TGCACCGCCA GTGCGCGTGG ATGAGGATGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGATGA CATTTTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATAGA ACTGAACGTA ATGCCAATAT CTGCTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGAGA TGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAATC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCGTCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTTCT 
GCTGACCAGC CCGCTCCCAG GAGAACATCT CGTCGAGTTT CTGCTCCGGA TAAAGATTCC 
TCACTGACCT CCTATACAAC AACAGCGTCA GATTCAGCAT CGGAAGACAG TATGAATCCT 
CCTCTTCTCA CAGAAGAGCT GAAATCTGAT CCTGATTCAC TTCTGGACCC AGAAAATCCC 
CTGCAGAAAA CACCTGTAGA CATAACAGAT GATAAAGAAA CACCCGACAA ACCCCAACTG 
CAAATAACAG AAGACGTCCA TATTGAATAT AACAAACCAG ATCTTACGCA AACCTCAAAG 
CCTGAAGTCG AAACGCCAAC CAGAGCGTCT GTGGTCCCGT TTAATACTCC AGACTACCGC 
TTTGAGTGCA TCTGTGGAGA GCTGGGCCTG GTGGACTATA AGCCCCGGGT TCAGTGTCTG 
AACTGTCTGT TATGGCAGCA CGCCGAGTGT GTCAACTATA AGGAGGAGAA TCTGGAAACG 
ACGCCGTTTT ATTGTCCTCA CTGTCTGGTG GCCATGACAC CAGTGTCCAC TGGAGCGACT 
CTTATCATCT CACCCAGCTC TATTTGTCAT CAGTGGGTGG ATGAAATCAA CCGGCACATT 
CACACCTCCT CCCTCAGAGT GCTGGTGTAT CAGGGCGTGA AGAGACACGG TTTTATCCAG 
CCTCACATTC TGGCCGAGCA GGATGTGGTC ATCACCACTT ATGACGTTTT GCGCACTGAA 
CTCAATTATG TGGATATTCC TCACAGCAAT AGTAAAGATG GCCGTCGATT CCGCAACCAG 
AAGCGCTACA TGGCCATACC GAGCCCTCTA GTGGCTGTGG AGTGGTGGCG CGTTTGTCTA 
GATGAAGCTC AGATGGTGGA GTGCACCACA GCCAAGGCTG CAGAAATGGC TTTGCGTCTC 
ACATCTGTTA ATCGCTGGTG TGTGAGTGGA ACACCTGTGC AGAGAGGTTT GGAGGACCTG 
TATGGTCTTG TCCTGTTCTT GGGAGTTGAT CCCTACTGGG TGAAGTACTG GTGGGATCAG 
TTACTCTATC GCCCGTACCG TCACGGCAAT ACAGCACCGC TCTACAACGT CATTGGGCAA 
CTACTATGGC GATCTGCCAA AAAAGACGTC ATTGATCAGA TCCAAATCCC CCCTCAAACA 
GAGGAGATCC ATTGGCTTAA CTTCTCCCCC GTGGAAAGAC ACTTTTACCA CCGTCAGCAT 
GAGGTTTGCT CCCAAGATGC TTTAGTGAAG CTGAGGAAGA TCTCTGATTG GTCACTGAAG 
CTGGGAAGTT TGGACCGTCG TACCGTCACT ACCATATTAT ACCCACTTTT GCGTCTGCGC 
CAGGCCTGCT GCCACCCGCA GGCTGTCCGA GGAGAATTTC TACCCCTCCA AAAAAGCACA 
ATGACAATGG AGGAGTTGTT GAAGTCGCTG CAGAAGAAAT GTCGTGTGGA GTGTGAAGAA 
GCGCACAGGC AGCTGGTCTG TGCTCTCAAC GGTCTGGCTG GAATCCACAT CATTAGAAGT 
GAGTTTGTAG ATGCAGCGGA GATGTACAGG GAAGTGTTGC GATCATCTGA AGAACACAAA 
GCAAGGCTGA AAACTGATTC CCTTCAGAGG CTTCATGCAA CCCATAATCT AATGGAACTT 
CTAAGTGCAA ACCACCCTGG AATTCCTCCA ACCCTGAGAG ATGACAGTCT CAAAGAAGAG 
GCCGAGCAGC TGAAGCAGCA TTACATGGCC AAAGTGAACT CTGAGGTTTC TGAAGCACAT 
CAAAACCTAC AGCCTGTTCT TCAGCACATT AAAGAACAAA GACGCAAGGT AAACCTTCGC 
TCCCCCTGGT GGCTGGATGT CATACAGCAG GCCATGCAGT ACTCCATGGA TGATGATCTT 
GTCAGCCGCA TTCAAAATGA GCTCACATGC AGTTATAAAC AACAAGCCAA CAAGCTCTCA 
ATGGCAGACA AATTTCGAGA CGCTCGGGGT CTTCAGTTTC TGCTCAGCAC TCAAATGGAT 
GATTTGATGA AGTCACAGAA AACAGTCCAG GATGCTGTGA AGAAGCTGGA AGGGCCGCCG 
TCTCAACAAG TCATCGAGGA AGCAACACTT TGCCACCTGC GACCTGTTCG CCTGCCACTG 
AACAACTGTG TGTTTTGCAA AGCAGATGAA CTTTTTACAG ACTACGAGTC TAAGCTTTTC 
TCGCACACAG TAAAAGGGCA GACAGCCATC TTTGAGGAGA TGATTGAAGA CGAGGAAGGG 
CTGGTAGATG ACAGGCTTCC CACCACCAGT AGAGGATTAT GGGCCGCCAG TGAGACCGAA 
CGAGCTCTTA AAGCCATCTT GTCCTTCGCT AAAGTCCGCC GCTTGGAGTC TGGCCTCATC 
GAGGAGGGAA ACTCCTTCAT GGAGCTCTTC GAGTCCTGGA AGAAAGAATA CAAGCTTCTG 
CATGAGTACT GGATGTCTCT GCGGGATCAT GTATCTGCTA TAGATGAGCT GGGAATGGCC 
ACCGAGAGGC TGCGTGTGCG CTTACCTGAT GAGCCCAAAC CCAAAGTCCT GCATATTATT 
GAGCCTCACG AGGTGGAGCA GAACAGGATA AAGCTGCTCA ATGACAGGGC AGTGGCCAAA 
TCACAGCTGC AAAAAAAGCT TGGGCAGTTT CTCTATCTCA CAAACCTGGA GAAGTCTCAG 
GATAAGTCCA CTGGTGGTCT GAATCCAGAA CCATGTCCAA TCTGTGCTCG TCCGCTGGGT 
CAGGAGTGGG CCGTACTGAC CTGTGGACAC TGTTTCTGTA ACGAGTGCAT CGCCATCATT 
GTGGAGCAAT ACAGCATCGG CAATCGACGG AGAGCTATAA AGTGTGCCAT ATGCCGACAA 
ACCACTTCAC ACGCAGAGAT CTCATATGTG TTCACCACTC AAAACAACCA CCAGGGGCAG 
GACATCCCTG TGAAGGGTAG CCACTCCACG AAGGTCGAGG CTGTGGTTCG AGTCCTAAAA 
AAAGTCCAGA TGACCGACCC GGGAGCCAAG TCTCTTGTGT TCTCCACGTG GCAGGGCGTC 
CTGGACATCA TCGCCAAAGC CCTGTTTGAC AACAACATGG AGTTTGCACA GATCAACGGA 
ATTCACAAGT TTCAGGAGAA CCTGAGTGCT TTCAAATATG AGGAGAAGAT CAACATCTTG 
TTGCTGCCAC TGCACACAGG CTCCAATGGA CTGAACATCA TTGAAGCAAC ACACGTGCTA 
CTGGTGGAGC CCATCCTAAA CCCCGCCCAC GAGCTGCAGG CCATAGGAAG AGTGCATCGG 
ATTGGCCAGA CCAAACCCAC ATTTGTTCAT CGGTTTCTGA TTAAGTCCAC CATTGAGGAA 
CGGATGCAAG CCATGCTGAA AACAGCAGAA AAGAGTCACA GTTCCACGTC TATGAAGCAT 
TCGGAGGCCT CTGTGTTTAC AGTGGCAGAC CTGGCAGATC TGTTCACCGA GGACAGCGAG 
ACTCTTGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328751.1
CDS	142..5073
Translation	MSSRRKRAPPVRVDEDAKRRLDWNMHEDRKNEGACDDILESVPAPSDSCLPVPVLEQGFISSAGTEEEPSEERPSCSTQDSPPDTIELNVMPISALDQGWNALIGEFNLHPKIPIEVADGSFCLQQTGDTLSMTLNTLEMEDEMGRSDPLCSVPVACSLGGLLLEDLDWLQKRKVIKLCHASGGVPVKLKVFLIESGLGRPEFLSEGNGRIKKASQLLQKLMELFYDFIIPEVMENEEEACDTDLERQNVEELYDHVRHLHQTEAAEQSFHVQHPALIPILRPYQSQAVNWMLRREKYKISSSPEQRLHFLWREMVTICGKKLFYNPYTGCLIREFPLAGVDWPGGILADEMGLGKTVEVLALILCNTRQNLEHGVLTLPVGRSVNYFVPPPPLERQRIIKHEPRPKEKIIYPAVREMLLTAIKEMKVGKGASINAIFAYLRTMYKYDTLKNRNHIKKILTKLISENIVEQIKGRGLAGSFKLGKNYKEKKKKPVTARTSADQPAPRRTSRRVSAPDKDSSLTSYTTTASDSASEDSMNPPLLTEELKSDPDSLLDPENPLQKTPVDITDDKETPDKPQLQITEDVHIEYNKPDLTQTSKPEVETPTRASVVPFNTPDYRFECICGELGLVDYKPRVQCLNCLLWQHAECVNYKEENLETTPFYCPHCLVAMTPVSTGATLIISPSSICHQWVDEINRHIHTSSLRVLVYQGVKRHGFIQPHILAEQDVVITTYDVLRTELNYVDIPHSNSKDGRRFRNQKRYMAIPSPLVAVEWWRVCLDEAQMVECTTAKAAEMALRLTSVNRWCVSGTPVQRGLEDLYGLVLFLGVDPYWVKYWWDQLLYRPYRHGNTAPLYNVIGQLLWRSAKKDVIDQIQIPPQTEEIHWLNFSPVERHFYHRQHEVCSQDALVKLRKISDWSLKLGSLDRRTVTTILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLKSLQKKCRVECEEAHRQLVCALNGLAGIHIIRSEFVDAAEMYREVLRSSEEHKARLKTDSLQRLHATHNLMELLSANHPGIPPTLRDDSLKEEAEQLKQHYMAKVNSEVSEAHQNLQPVLQHIKEQRRKVNLRSPWWLDVIQQAMQYSMDDDLVSRIQNELTCSYKQQANKLSMADKFRDARGLQFLLSTQMDDLMKSQKTVQDAVKKLEGPPSQQVIEEATLCHLRPVRLPLNNCVFCKADELFTDYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRLPTTSRGLWAASETERALKAILSFAKVRRLESGLIEEGNSFMELFESWKKEYKLLHEYWMSLRDHVSAIDELGMATERLRVRLPDEPKPKVLHIIEPHEVEQNRIKLLNDRAVAKSQLQKKLGQFLYLTNLEKSQDKSTGGLNPEPCPICARPLGQEWAVLTCGHCFCNECIAIIVEQYSIGNRRRAIKCAICRQTTSHAEISYVFTTQNNHQGQDIPVKGSHSTKVEAVVRVLKKVQMTDPGAKSLVFSTWQGVLDIIAKALFDNNMEFAQINGIHKFQENLSAFKYEEKINILLLPLHTGSNGLNIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTFVHRFLIKSTIEERMQAMLKTAEKSHSSTSMKHSEASVFTVADLADLFTEDSETLE

Target ORF information:

RefSeq Version	XM_021473076.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473076.1

ORF Insert Sequence:

ATGAGCAGCA GGAGGAAACG TGCACCGCCA GTGCGCGTGG ATGAGGATGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGATGA CATTTTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATAGA ACTGAACGTA ATGCCAATAT CTGCTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGAGA TGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAATC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCGTCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTTCT 
GCTGACCAGC CCGCTCCCAG GAGAACATCT CGTCGAGTTT CTGCTCCGGA TAAAGATTCC 
TCACTGACCT CCTATACAAC AACAGCGTCA GATTCAGCAT CGGAAGACAG TATGAATCCT 
CCTCTTCTCA CAGAAGAGCT GAAATCTGAT CCTGATTCAC TTCTGGACCC AGAAAATCCC 
CTGCAGAAAA CACCTGTAGA CATAACAGAT GATAAAGAAA CACCCGACAA ACCCCAACTG 
CAAATAACAG AAGACGTCCA TATTGAATAT AACAAACCAG ATCTTACGCA AACCTCAAAG 
CCTGAAGTCG AAACGCCAAC CAGAGCGTCT GTGGTCCCGT TTAATACTCC AGACTACCGC 
TTTGAGTGCA TCTGTGGAGA GCTGGGCCTG GTGGACTATA AGCCCCGGGT TCAGTGTCTG 
AACTGTCTGT TATGGCAGCA CGCCGAGTGT GTCAACTATA AGGAGGAGAA TCTGGAAACG 
ACGCCGTTTT ATTGTCCTCA CTGTCTGGTG GCCATGACAC CAGTGTCCAC TGGAGCGACT 
CTTATCATCT CACCCAGCTC TATTTGTCAT CAGTGGGTGG ATGAAATCAA CCGGCACATT 
CACACCTCCT CCCTCAGAGT GCTGGTGTAT CAGGGCGTGA AGAGACACGG TTTTATCCAG 
CCTCACATTC TGGCCGAGCA GGATGTGGTC ATCACCACTT ATGACGTTTT GCGCACTGAA 
CTCAATTATG TGGATATTCC TCACAGCAAT AGTAAAGATG GCCGTCGATT CCGCAACCAG 
AAGCGCTACA TGGCCATACC GAGCCCTCTA GTGGCTGTGG AGTGGTGGCG CGTTTGTCTA 
GATGAAGCTC AGATGGTGGA GTGCACCACA GCCAAGGCTG CAGAAATGGC TTTGCGTCTC 
ACATCTGTTA ATCGCTGGTG TGTGAGTGGA ACACCTGTGC AGAGAGGTTT GGAGGACCTG 
TATGGTCTTG TCCTGTTCTT GGGAGTTGAT CCCTACTGGG TGAAGTACTG GTGGGATCAG 
TTACTCTATC GCCCGTACCG TCACGGCAAT ACAGCACCGC TCTACAACGT CATTGGGCAA 
CTACTATGGC GATCTGCCAA AAAAGACGTC ATTGATCAGA TCCAAATCCC CCCTCAAACA 
GAGGAGATCC ATTGGCTTAA CTTCTCCCCC GTGGAAAGAC ACTTTTACCA CCGTCAGCAT 
GAGGTTTGCT CCCAAGATGC TTTAGTGAAG CTGAGGAAGA TCTCTGATTG GTCACTGAAG 
CTGGGAAGTT TGGACCGTCG TACCGTCACT ACCATATTAT ACCCACTTTT GCGTCTGCGC 
CAGGCCTGCT GCCACCCGCA GGCTGTCCGA GGAGAATTTC TACCCCTCCA AAAAAGCACA 
ATGACAATGG AGGAGTTGTT GAAGTCGCTG CAGAAGAAAT GTCGTGTGGA GTGTGAAGAA 
GCGCACAGGC AGCTGGTCTG TGCTCTCAAC GGTCTGGCTG GAATCCACAT CATTAGAAGT 
GAGTTTGTAG ATGCAGCGGA GATGTACAGG GAAGTGTTGC GATCATCTGA AGAACACAAA 
GCAAGGCTGA AAACTGATTC CCTTCAGAGG CTTCATGCAA CCCATAATCT AATGGAACTT 
CTAAGTGCAA ACCACCCTGG AATTCCTCCA ACCCTGAGAG ATGACAGTCT CAAAGAAGAG 
GCCGAGCAGC TGAAGCAGCA TTACATGGCC AAAGTGAACT CTGAGGTTTC TGAAGCACAT 
CAAAACCTAC AGCCTGTTCT TCAGCACATT AAAGAACAAA GACGCAAGGT AAACCTTCGC 
TCCCCCTGGT GGCTGGATGT CATACAGCAG GCCATGCAGT ACTCCATGGA TGATGATCTT 
GTCAGCCGCA TTCAAAATGA GCTCACATGC AGTTATAAAC AACAAGCCAA CAAGCTCTCA 
ATGGCAGACA AATTTCGAGA CGCTCGGGGT CTTCAGTTTC TGCTCAGCAC TCAAATGGAT 
GATTTGATGA AGTCACAGAA AACAGTCCAG GATGCTGTGA AGAAGCTGGA AGGGCCGCCG 
TCTCAACAAG TCATCGAGGA AGCAACACTT TGCCACCTGC GACCTGTTCG CCTGCCACTG 
AACAACTGTG TGTTTTGCAA AGCAGATGAA CTTTTTACAG ACTACGAGTC TAAGCTTTTC 
TCGCACACAG TAAAAGGGCA GACAGCCATC TTTGAGGAGA TGATTGAAGA CGAGGAAGGG 
CTGGTAGATG ACAGGCTTCC CACCACCAGT AGAGGATTAT GGGCCGCCAG TGAGACCGAA 
CGAGCTCTTA AAGCCATCTT GTCCTTCGCT AAAGTCCGCC GCTTGGAGTC TGGCCTCATC 
GAGGAGGGAA ACTCCTTCAT GGAGCTCTTC GAGTCCTGGA AGAAAGAATA CAAGCTTCTG 
CATGAGTACT GGATGTCTCT GCGGGATCAT GTATCTGCTA TAGATGAGCT GGGAATGGCC 
ACCGAGAGGC TGCGTGTGCG CTTACCTGAT GAGCCCAAAC CCAAAGTCCT GCATATTATT 
GAGCCTCACG AGGTGGAGCA GAACAGGATA AAGCTGCTCA ATGACAGGGC AGTGGCCAAA 
TCACAGCTGC AAAAAAAGCT TGGGCAGTTT CTCTATCTCA CAAACCTGGA GAAGTCTCAG 
GATAAGTCCA CTGGTGGTCT GAATCCAGAA CCATGTCCAA TCTGTGCTCG TCCGCTGGGT 
CAGGAGTGGG CCGTACTGAC CTGTGGACAC TGTTTCTGTA ACGAGTGCAT CGCCATCATT 
GTGGAGCAAT ACAGCATCGG CAATCGACGG AGAGCTATAA AGTGTGCCAT ATGCCGACAA 
ACCACTTCAC ACGCAGAGAT CTCATATGTG TTCACCACTC AAAACAACCA CCAGGGGCAG 
GACATCCCTG TGAAGGGTAG CCACTCCACG AAGGTCGAGG CTGTGGTTCG AGTCCTAAAA 
AAAGTCCAGA TGACCGACCC GGGAGCCAAG TCTCTTGTGT TCTCCACGTG GCAGGGCGTC 
CTGGACATCA TCGCCAAAGC CCTGTTTGAC AACAACATGG AGTTTGCACA GATCAACGGA 
ATTCACAAGT TTCAGGAGAA CCTGAGTGCT TTCAAATATG AGGAGAAGAT CAACATCTTG 
TTGCTGCCAC TGCACACAGG CTCCAATGGA CTGAACATCA TTGAAGCAAC ACACGTGCTA 
CTGGTGGAGC CCATCCTAAA CCCCGCCCAC GAGCTGCAGG CCATAGGAAG AGTGCATCGG 
ATTGGCCAGA CCAAACCCAC ATTTGTTCAT CGGTTTCTGA TTAAGTCCAC CATTGAGGAA 
CGGATGCAAG CCATGCTGAA AACAGCAGAA AAGAGTCACA GTTCCACGTC TATGAAGCAT 
TCGGAGGCCT CTGTGTTTAC AGTGGCAGAC CTGGCAGATC TGTTCACCGA GGACAGCGAG 
ACTCTTGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shprh ( XM_003200310.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200358.1 Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22222
Clone ID Related Accession (Same CDS sequence)	XM_003200310.4 , XM_003200310.5
Accession Version	XM_003200310.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase SHPRH isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200310.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA GGAGGAAACG TGCGCCGCCA GTGCGCGTGG ATGAGGACGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGAAGA CATTCTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATTGA ACTGAATGTA ATGCCAATTT CTGTTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGGGA GGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAAGC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCATCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTAAG 
TCTGCTGACC AGCCCGCTCC CAGGAGAACA TCTCGTCGAG TTTCTGCTCC GGATAAAGAT 
TCCTCACTGA CCTCCTATAC AACAACAGCA TCAGATTCAG CATCGGAAGA CAGTATGAAT 
CCTCCTCTTC TCACAGAAGA GCTGAAATCT GATCCTGATT CACTTCTGGA CCCAGAAAAT 
CCCCTGCAGA AAAAACCTGT AGACATAACA GATGATAAAG AAATACCCGA CAAACCCCAA 
CTGCAAATAA CAGAAGACGT CCATATTGAA TCTAACAAAC CAGATCTTAT GCAAACCTCA 
AAACCTGAAG TCGAAACGCC AACCAGAGCG TCTGTGGTCC CGTTTAATAC TCCAGACTAC 
CGCTTTGAGT GCATCTGTGG AGAGCTGGGC CTGGTGGACT ATAAGCCCCG GGTTCAGTGT 
CTGAACTGTC TGTTATGGCA GCATGCCGAG TGTGTCAACT ATAAGGAGGA GAATCTGGAA 
ACGACGCCAT TTTATTGTCC TCACTGTCTG GTGGCCATGA CACCAGTGTC CACTGGAGCG 
ACTCTTATCA TCTCACCCAG CTCAATTTGT CATCAGTGGG TGGATGAAAT CAACCGGCAC 
ATTCACACCT CCTCCCTCAG AGTGCTGGTG TATCAGGGCG TGAAGAGACA CGGTTTTATC 
CAGCCTCACA TTCTGGCCGA GCAGGATGTG GTCATCACCA CTTATGACGT TCTGCGCACT 
GAACTCAATT ATGTGGATAT TCCTCACAGC AATAGTAAAG ATGGCCGTCG ATTCCGCAAC 
CAGAAGCGCT ACATGGCCAT ACCGAGCCCT CTAGTGGCTG TGGAGTGGTG GCGCGTTTGT 
CTAGATGAAG CTCAGATGGT GGAGTGCACC ACAGCCAAGG CTGCAGAAAT GGCTTTGCGT 
CTCACATCTG TTAATCGCTG GTGTGTGAGT GGAACACCTG TGCAGAGAGG TTTGGAGGAC 
CTGTATGGTC TCGTCCTGTT CTTGGGAGTT GATCCCTACT GGGTGAAGTA CTGGTGGGAT 
CAGTTACTCT ATCGCCCGTA CCGTCACGGC AATACAGCAC CGCTCTACAA CGTCATTGGT 
CAACTACTAT GGCGATCTGC CAAAAAAGAC GTCATTGATC AGATCCAAAT CCCCCCTCAA 
ACAGAGGAGA TCCATTGGCT TAACTTCTCC CCCGTGGAAA GACACTTTTA CCACCGTCAG 
CATGAGGTTT GCTCCCAGGA TGCTTTAGTG AAGCTAAGGA AGATCTCAGA TTGGTCACTG 
AAGCTAGGAA GTTTGGACCG TCGTACCGTC ACTACCATAT TATACCCACT TTTGCGTCTG 
CGCCAGGCCT GCTGCCACCC GCAGGCCGTC CGAGGAGAAT TTCTACCCCT TCAAAAAAGC 
ACAATGACAA TGGAGGAGTT GTTGAAGTCG CTGCAGAAGA AATGTCGTGT GGAGTGTGAA 
GAAGCGCACA GGCAGCTGGT CTGTGCTCTC AACGGTCTGG CTGGAATCCA CATCATTAGA 
AGTGAGTTTG TAGATGCAGC GGAGATGTAC AGGGAAGTGT TGCGATCATC TGAAGAACAC 
AAAGCAAGGC TGAAAACTGA TTCCCTTCAG AGGCTTCATG CAACCCACAA TCTAATGGAA 
CTTCTAAGTG CAAACCACCC TGGGATTCCT CCAACCCTGA GAGATGACAG TCTCAAAGAA 
GAGGCCGAGC AGCTGAAGCA GCATTACATG GCCAAAGTGA ACTCTGAGGT TTCTGAAGCA 
CATCAAAACC TACAGCCTGT TCTTCAGCAC ATTAAAGAAC AAAGACGCAA GGTAAACCTT 
CGCTCCCCCT GGTGGCTGGA TGTCATACAG CAGGCCATGC AGTACTCCAT GGATGATGAT 
CTTGTCAGCC GCATTCAAAA TGAGCTTACA TGCAGTTATA AACAACAAGC CAACAAGCTC 
TCAATGGCAG ACAAATTTCG AGACGCTCGG GGTCTTCAGT TTCTGCTCAG CACTCAAATG 
GATGATTTGA TGAAGTCACA GAAAACAGTC CAGGATGCTG TGAAGAAGCT GGAAGGGCCG 
CCGTCTCAAC AAGTCATCGA GGAAGCAACA CTTTGCCACC TGCGACCTGT TCGTCTGCCA 
CTGAACAACT GTGTGTTTTG CAAAGCAGAT GAACTTTTTA CAGACTACGA GTCGAAGCTT 
TTCTCGCACA CAGTAAAAGG GCAGACGGCT ATCTTTGAGG AGATGATTGA AGACGAGGAA 
GGGCTGGTAG ATGACAGGCT TCCCACCACC AGTAGAGGAT TATGGGCCGC CAGTGAGACC 
GAACGAGCTC TTAAAGCCAT CTTGTCCTTC GCTAAAGTCC GCCGCTTGGA GTCTGGCCTC 
ATCGAGGAGG GAAACTCCTT CATGGAGCTC TTCGAGTCCT GGAAGAAAGA ATACAAGCTT 
CTGCATGAGT ACTGGATGTC TCTGCGGGAT CATGTATCTG CTATAGATGA GCTGGGAATG 
GCCACCGAGA GGCTGCGTGT GCGCTTACCT GATGAGCCCA AACCCAAAGT CCTGCATATT 
ATTGAGCCTC ACGAGGTGGA GCAGAACAGG ATAAAGCTGC TCAATGACAG GGCAGTGGCC 
AAATCACAGC TGCAAAAAAA GCTTGGGCAG TTTCTCTATC TCACAAACCT GGAGAAGTCT 
CAGGATAAGT CCACTGGTGG TCTGAATCCA GAACCATGTC CAATCTGTGC TCGTCCGCTG 
GGTCAGGAGT GGGCCGTACT GACCTGTGGA CACTGTTTCT GTAACGAGTG CATCGCCATC 
ATTGTGGAGC AATACAGCAT CGGCAATCGA CGGAGAGCTA TAAAGTGTGC CATATGCCGA 
CAAACCACTT CACACGCAGA GATCTCATAT GTGTTCACCA CTCAAAACAA CCACCAGGGG 
CAGGACATCC CTGTGAAGGG TAGCCACTCC ACGAAGGTCG AGGCTGTGGT TCGAGTCCTA 
AAAAAAGTCC AGATGACCGA CCCGGGAGCC AAGTCTCTTG TGTTCTCCAC GTGGCAGGGC 
GTCCTGGACA TCATCGCCAA AGCCCTGTTT GACAACAACA TGGAGTTTGC ACAGATCAAC 
GGAATTCACA AGTTTCAGGA GAACCTGAGT GCTTTCAAAT ATGAGGAGAA GATCAACATC 
TTGTTGCTGC CACTGCACAC AGGCTCCAAT GGACTGAACA TCATTGAAGC AACACACGTG 
CTACTGGTGG AGCCCATCCT AAACCCCGCC CACGAGCTGC AGGCCATAGG AAGAGTGCAT 
CGGATTGGCC AGACCAAACC CACATTTGTT CATCGGTTTC TGATTAAGTC CACCATTGAG 
GAACGGATGC AAGCCATGCT GAAAACAGCA GAAAAGAGTC ACAGTTCCAC GTCTATGAAG 
CATTCGGAGG CCTCTGTGTT TACAGTGGCA GACCTGGCGG ATCTGTTCAC CGAGGACAGC 
GAGACTCTTG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200358.1
CDS	122..5056
Translation	MSSRRKRAPPVRVDEDAKRRLDWNMHEDRKNEGACEDILESVPAPSDSCLPVPVLEQGFISSAGTEEEPSEERPSCSTQDSPPDTIELNVMPISVLDQGWNALIGEFNLHPKIPIEVADGSFCLQQTGDTLSMTLNTLEMEDGRGRSDPLCSVPVACSLGGLLLEDLDWLQKRKVIKLCHASGGVPVKLKVFLIESGLGRPEFLSEGNGRIKKASQLLQKLMELFYDFIIPEVMENEEEACDTDLERQNVEELYDHVRHLHQTEAAEQSFHVQHPALIPILRPYQSQAVNWMLRREKYKSSSSPEQRLHFLWREMVTICGKKLFYNPYTGCLIREFPLAGVDWPGGILADEMGLGKTVEVLALILCNTRQNLEHGVLTLPVGRSVNYFVPPPPLERQRIIKHEPRPKEKIIYPAVREMLLTAIKEMKVGKGASINAIFAYLRTMYKYDTLKNRNHIKKILTKLISENIVEQIKGRGLAGSFKLGKNYKEKKKKPVTARTKSADQPAPRRTSRRVSAPDKDSSLTSYTTTASDSASEDSMNPPLLTEELKSDPDSLLDPENPLQKKPVDITDDKEIPDKPQLQITEDVHIESNKPDLMQTSKPEVETPTRASVVPFNTPDYRFECICGELGLVDYKPRVQCLNCLLWQHAECVNYKEENLETTPFYCPHCLVAMTPVSTGATLIISPSSICHQWVDEINRHIHTSSLRVLVYQGVKRHGFIQPHILAEQDVVITTYDVLRTELNYVDIPHSNSKDGRRFRNQKRYMAIPSPLVAVEWWRVCLDEAQMVECTTAKAAEMALRLTSVNRWCVSGTPVQRGLEDLYGLVLFLGVDPYWVKYWWDQLLYRPYRHGNTAPLYNVIGQLLWRSAKKDVIDQIQIPPQTEEIHWLNFSPVERHFYHRQHEVCSQDALVKLRKISDWSLKLGSLDRRTVTTILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLKSLQKKCRVECEEAHRQLVCALNGLAGIHIIRSEFVDAAEMYREVLRSSEEHKARLKTDSLQRLHATHNLMELLSANHPGIPPTLRDDSLKEEAEQLKQHYMAKVNSEVSEAHQNLQPVLQHIKEQRRKVNLRSPWWLDVIQQAMQYSMDDDLVSRIQNELTCSYKQQANKLSMADKFRDARGLQFLLSTQMDDLMKSQKTVQDAVKKLEGPPSQQVIEEATLCHLRPVRLPLNNCVFCKADELFTDYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRLPTTSRGLWAASETERALKAILSFAKVRRLESGLIEEGNSFMELFESWKKEYKLLHEYWMSLRDHVSAIDELGMATERLRVRLPDEPKPKVLHIIEPHEVEQNRIKLLNDRAVAKSQLQKKLGQFLYLTNLEKSQDKSTGGLNPEPCPICARPLGQEWAVLTCGHCFCNECIAIIVEQYSIGNRRRAIKCAICRQTTSHAEISYVFTTQNNHQGQDIPVKGSHSTKVEAVVRVLKKVQMTDPGAKSLVFSTWQGVLDIIAKALFDNNMEFAQINGIHKFQENLSAFKYEEKINILLLPLHTGSNGLNIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTFVHRFLIKSTIEERMQAMLKTAEKSHSSTSMKHSEASVFTVADLADLFTEDSETLE

Target ORF information:

RefSeq Version	XM_003200310.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200310.4

ORF Insert Sequence:

ATGAGCAGCA GGAGGAAACG TGCGCCGCCA GTGCGCGTGG ATGAGGACGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGAAGA CATTCTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATTGA ACTGAATGTA ATGCCAATTT CTGTTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGGGA GGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAAGC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCATCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTAAG 
TCTGCTGACC AGCCCGCTCC CAGGAGAACA TCTCGTCGAG TTTCTGCTCC GGATAAAGAT 
TCCTCACTGA CCTCCTATAC AACAACAGCA TCAGATTCAG CATCGGAAGA CAGTATGAAT 
CCTCCTCTTC TCACAGAAGA GCTGAAATCT GATCCTGATT CACTTCTGGA CCCAGAAAAT 
CCCCTGCAGA AAAAACCTGT AGACATAACA GATGATAAAG AAATACCCGA CAAACCCCAA 
CTGCAAATAA CAGAAGACGT CCATATTGAA TCTAACAAAC CAGATCTTAT GCAAACCTCA 
AAACCTGAAG TCGAAACGCC AACCAGAGCG TCTGTGGTCC CGTTTAATAC TCCAGACTAC 
CGCTTTGAGT GCATCTGTGG AGAGCTGGGC CTGGTGGACT ATAAGCCCCG GGTTCAGTGT 
CTGAACTGTC TGTTATGGCA GCATGCCGAG TGTGTCAACT ATAAGGAGGA GAATCTGGAA 
ACGACGCCAT TTTATTGTCC TCACTGTCTG GTGGCCATGA CACCAGTGTC CACTGGAGCG 
ACTCTTATCA TCTCACCCAG CTCAATTTGT CATCAGTGGG TGGATGAAAT CAACCGGCAC 
ATTCACACCT CCTCCCTCAG AGTGCTGGTG TATCAGGGCG TGAAGAGACA CGGTTTTATC 
CAGCCTCACA TTCTGGCCGA GCAGGATGTG GTCATCACCA CTTATGACGT TCTGCGCACT 
GAACTCAATT ATGTGGATAT TCCTCACAGC AATAGTAAAG ATGGCCGTCG ATTCCGCAAC 
CAGAAGCGCT ACATGGCCAT ACCGAGCCCT CTAGTGGCTG TGGAGTGGTG GCGCGTTTGT 
CTAGATGAAG CTCAGATGGT GGAGTGCACC ACAGCCAAGG CTGCAGAAAT GGCTTTGCGT 
CTCACATCTG TTAATCGCTG GTGTGTGAGT GGAACACCTG TGCAGAGAGG TTTGGAGGAC 
CTGTATGGTC TCGTCCTGTT CTTGGGAGTT GATCCCTACT GGGTGAAGTA CTGGTGGGAT 
CAGTTACTCT ATCGCCCGTA CCGTCACGGC AATACAGCAC CGCTCTACAA CGTCATTGGT 
CAACTACTAT GGCGATCTGC CAAAAAAGAC GTCATTGATC AGATCCAAAT CCCCCCTCAA 
ACAGAGGAGA TCCATTGGCT TAACTTCTCC CCCGTGGAAA GACACTTTTA CCACCGTCAG 
CATGAGGTTT GCTCCCAGGA TGCTTTAGTG AAGCTAAGGA AGATCTCAGA TTGGTCACTG 
AAGCTAGGAA GTTTGGACCG TCGTACCGTC ACTACCATAT TATACCCACT TTTGCGTCTG 
CGCCAGGCCT GCTGCCACCC GCAGGCCGTC CGAGGAGAAT TTCTACCCCT TCAAAAAAGC 
ACAATGACAA TGGAGGAGTT GTTGAAGTCG CTGCAGAAGA AATGTCGTGT GGAGTGTGAA 
GAAGCGCACA GGCAGCTGGT CTGTGCTCTC AACGGTCTGG CTGGAATCCA CATCATTAGA 
AGTGAGTTTG TAGATGCAGC GGAGATGTAC AGGGAAGTGT TGCGATCATC TGAAGAACAC 
AAAGCAAGGC TGAAAACTGA TTCCCTTCAG AGGCTTCATG CAACCCACAA TCTAATGGAA 
CTTCTAAGTG CAAACCACCC TGGGATTCCT CCAACCCTGA GAGATGACAG TCTCAAAGAA 
GAGGCCGAGC AGCTGAAGCA GCATTACATG GCCAAAGTGA ACTCTGAGGT TTCTGAAGCA 
CATCAAAACC TACAGCCTGT TCTTCAGCAC ATTAAAGAAC AAAGACGCAA GGTAAACCTT 
CGCTCCCCCT GGTGGCTGGA TGTCATACAG CAGGCCATGC AGTACTCCAT GGATGATGAT 
CTTGTCAGCC GCATTCAAAA TGAGCTTACA TGCAGTTATA AACAACAAGC CAACAAGCTC 
TCAATGGCAG ACAAATTTCG AGACGCTCGG GGTCTTCAGT TTCTGCTCAG CACTCAAATG 
GATGATTTGA TGAAGTCACA GAAAACAGTC CAGGATGCTG TGAAGAAGCT GGAAGGGCCG 
CCGTCTCAAC AAGTCATCGA GGAAGCAACA CTTTGCCACC TGCGACCTGT TCGTCTGCCA 
CTGAACAACT GTGTGTTTTG CAAAGCAGAT GAACTTTTTA CAGACTACGA GTCGAAGCTT 
TTCTCGCACA CAGTAAAAGG GCAGACGGCT ATCTTTGAGG AGATGATTGA AGACGAGGAA 
GGGCTGGTAG ATGACAGGCT TCCCACCACC AGTAGAGGAT TATGGGCCGC CAGTGAGACC 
GAACGAGCTC TTAAAGCCAT CTTGTCCTTC GCTAAAGTCC GCCGCTTGGA GTCTGGCCTC 
ATCGAGGAGG GAAACTCCTT CATGGAGCTC TTCGAGTCCT GGAAGAAAGA ATACAAGCTT 
CTGCATGAGT ACTGGATGTC TCTGCGGGAT CATGTATCTG CTATAGATGA GCTGGGAATG 
GCCACCGAGA GGCTGCGTGT GCGCTTACCT GATGAGCCCA AACCCAAAGT CCTGCATATT 
ATTGAGCCTC ACGAGGTGGA GCAGAACAGG ATAAAGCTGC TCAATGACAG GGCAGTGGCC 
AAATCACAGC TGCAAAAAAA GCTTGGGCAG TTTCTCTATC TCACAAACCT GGAGAAGTCT 
CAGGATAAGT CCACTGGTGG TCTGAATCCA GAACCATGTC CAATCTGTGC TCGTCCGCTG 
GGTCAGGAGT GGGCCGTACT GACCTGTGGA CACTGTTTCT GTAACGAGTG CATCGCCATC 
ATTGTGGAGC AATACAGCAT CGGCAATCGA CGGAGAGCTA TAAAGTGTGC CATATGCCGA 
CAAACCACTT CACACGCAGA GATCTCATAT GTGTTCACCA CTCAAAACAA CCACCAGGGG 
CAGGACATCC CTGTGAAGGG TAGCCACTCC ACGAAGGTCG AGGCTGTGGT TCGAGTCCTA 
AAAAAAGTCC AGATGACCGA CCCGGGAGCC AAGTCTCTTG TGTTCTCCAC GTGGCAGGGC 
GTCCTGGACA TCATCGCCAA AGCCCTGTTT GACAACAACA TGGAGTTTGC ACAGATCAAC 
GGAATTCACA AGTTTCAGGA GAACCTGAGT GCTTTCAAAT ATGAGGAGAA GATCAACATC 
TTGTTGCTGC CACTGCACAC AGGCTCCAAT GGACTGAACA TCATTGAAGC AACACACGTG 
CTACTGGTGG AGCCCATCCT AAACCCCGCC CACGAGCTGC AGGCCATAGG AAGAGTGCAT 
CGGATTGGCC AGACCAAACC CACATTTGTT CATCGGTTTC TGATTAAGTC CACCATTGAG 
GAACGGATGC AAGCCATGCT GAAAACAGCA GAAAAGAGTC ACAGTTCCAC GTCTATGAAG 
CATTCGGAGG CCTCTGTGTT TACAGTGGCA GACCTGGCGG ATCTGTTCAC CGAGGACAGC 
GAGACTCTTG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shprh ( XM_003200310.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200358.1 Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X1, mRNA.

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CloneID	ODa22222
Clone ID Related Accession (Same CDS sequence)	XM_003200310.4 , XM_003200310.5
Accession Version	XM_003200310.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase SHPRH isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200310.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA GGAGGAAACG TGCGCCGCCA GTGCGCGTGG ATGAGGACGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGAAGA CATTCTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATTGA ACTGAATGTA ATGCCAATTT CTGTTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGGGA GGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAAGC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCATCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTAAG 
TCTGCTGACC AGCCCGCTCC CAGGAGAACA TCTCGTCGAG TTTCTGCTCC GGATAAAGAT 
TCCTCACTGA CCTCCTATAC AACAACAGCA TCAGATTCAG CATCGGAAGA CAGTATGAAT 
CCTCCTCTTC TCACAGAAGA GCTGAAATCT GATCCTGATT CACTTCTGGA CCCAGAAAAT 
CCCCTGCAGA AAAAACCTGT AGACATAACA GATGATAAAG AAATACCCGA CAAACCCCAA 
CTGCAAATAA CAGAAGACGT CCATATTGAA TCTAACAAAC CAGATCTTAT GCAAACCTCA 
AAACCTGAAG TCGAAACGCC AACCAGAGCG TCTGTGGTCC CGTTTAATAC TCCAGACTAC 
CGCTTTGAGT GCATCTGTGG AGAGCTGGGC CTGGTGGACT ATAAGCCCCG GGTTCAGTGT 
CTGAACTGTC TGTTATGGCA GCATGCCGAG TGTGTCAACT ATAAGGAGGA GAATCTGGAA 
ACGACGCCAT TTTATTGTCC TCACTGTCTG GTGGCCATGA CACCAGTGTC CACTGGAGCG 
ACTCTTATCA TCTCACCCAG CTCAATTTGT CATCAGTGGG TGGATGAAAT CAACCGGCAC 
ATTCACACCT CCTCCCTCAG AGTGCTGGTG TATCAGGGCG TGAAGAGACA CGGTTTTATC 
CAGCCTCACA TTCTGGCCGA GCAGGATGTG GTCATCACCA CTTATGACGT TCTGCGCACT 
GAACTCAATT ATGTGGATAT TCCTCACAGC AATAGTAAAG ATGGCCGTCG ATTCCGCAAC 
CAGAAGCGCT ACATGGCCAT ACCGAGCCCT CTAGTGGCTG TGGAGTGGTG GCGCGTTTGT 
CTAGATGAAG CTCAGATGGT GGAGTGCACC ACAGCCAAGG CTGCAGAAAT GGCTTTGCGT 
CTCACATCTG TTAATCGCTG GTGTGTGAGT GGAACACCTG TGCAGAGAGG TTTGGAGGAC 
CTGTATGGTC TCGTCCTGTT CTTGGGAGTT GATCCCTACT GGGTGAAGTA CTGGTGGGAT 
CAGTTACTCT ATCGCCCGTA CCGTCACGGC AATACAGCAC CGCTCTACAA CGTCATTGGT 
CAACTACTAT GGCGATCTGC CAAAAAAGAC GTCATTGATC AGATCCAAAT CCCCCCTCAA 
ACAGAGGAGA TCCATTGGCT TAACTTCTCC CCCGTGGAAA GACACTTTTA CCACCGTCAG 
CATGAGGTTT GCTCCCAGGA TGCTTTAGTG AAGCTAAGGA AGATCTCAGA TTGGTCACTG 
AAGCTAGGAA GTTTGGACCG TCGTACCGTC ACTACCATAT TATACCCACT TTTGCGTCTG 
CGCCAGGCCT GCTGCCACCC GCAGGCCGTC CGAGGAGAAT TTCTACCCCT TCAAAAAAGC 
ACAATGACAA TGGAGGAGTT GTTGAAGTCG CTGCAGAAGA AATGTCGTGT GGAGTGTGAA 
GAAGCGCACA GGCAGCTGGT CTGTGCTCTC AACGGTCTGG CTGGAATCCA CATCATTAGA 
AGTGAGTTTG TAGATGCAGC GGAGATGTAC AGGGAAGTGT TGCGATCATC TGAAGAACAC 
AAAGCAAGGC TGAAAACTGA TTCCCTTCAG AGGCTTCATG CAACCCACAA TCTAATGGAA 
CTTCTAAGTG CAAACCACCC TGGGATTCCT CCAACCCTGA GAGATGACAG TCTCAAAGAA 
GAGGCCGAGC AGCTGAAGCA GCATTACATG GCCAAAGTGA ACTCTGAGGT TTCTGAAGCA 
CATCAAAACC TACAGCCTGT TCTTCAGCAC ATTAAAGAAC AAAGACGCAA GGTAAACCTT 
CGCTCCCCCT GGTGGCTGGA TGTCATACAG CAGGCCATGC AGTACTCCAT GGATGATGAT 
CTTGTCAGCC GCATTCAAAA TGAGCTTACA TGCAGTTATA AACAACAAGC CAACAAGCTC 
TCAATGGCAG ACAAATTTCG AGACGCTCGG GGTCTTCAGT TTCTGCTCAG CACTCAAATG 
GATGATTTGA TGAAGTCACA GAAAACAGTC CAGGATGCTG TGAAGAAGCT GGAAGGGCCG 
CCGTCTCAAC AAGTCATCGA GGAAGCAACA CTTTGCCACC TGCGACCTGT TCGTCTGCCA 
CTGAACAACT GTGTGTTTTG CAAAGCAGAT GAACTTTTTA CAGACTACGA GTCGAAGCTT 
TTCTCGCACA CAGTAAAAGG GCAGACGGCT ATCTTTGAGG AGATGATTGA AGACGAGGAA 
GGGCTGGTAG ATGACAGGCT TCCCACCACC AGTAGAGGAT TATGGGCCGC CAGTGAGACC 
GAACGAGCTC TTAAAGCCAT CTTGTCCTTC GCTAAAGTCC GCCGCTTGGA GTCTGGCCTC 
ATCGAGGAGG GAAACTCCTT CATGGAGCTC TTCGAGTCCT GGAAGAAAGA ATACAAGCTT 
CTGCATGAGT ACTGGATGTC TCTGCGGGAT CATGTATCTG CTATAGATGA GCTGGGAATG 
GCCACCGAGA GGCTGCGTGT GCGCTTACCT GATGAGCCCA AACCCAAAGT CCTGCATATT 
ATTGAGCCTC ACGAGGTGGA GCAGAACAGG ATAAAGCTGC TCAATGACAG GGCAGTGGCC 
AAATCACAGC TGCAAAAAAA GCTTGGGCAG TTTCTCTATC TCACAAACCT GGAGAAGTCT 
CAGGATAAGT CCACTGGTGG TCTGAATCCA GAACCATGTC CAATCTGTGC TCGTCCGCTG 
GGTCAGGAGT GGGCCGTACT GACCTGTGGA CACTGTTTCT GTAACGAGTG CATCGCCATC 
ATTGTGGAGC AATACAGCAT CGGCAATCGA CGGAGAGCTA TAAAGTGTGC CATATGCCGA 
CAAACCACTT CACACGCAGA GATCTCATAT GTGTTCACCA CTCAAAACAA CCACCAGGGG 
CAGGACATCC CTGTGAAGGG TAGCCACTCC ACGAAGGTCG AGGCTGTGGT TCGAGTCCTA 
AAAAAAGTCC AGATGACCGA CCCGGGAGCC AAGTCTCTTG TGTTCTCCAC GTGGCAGGGC 
GTCCTGGACA TCATCGCCAA AGCCCTGTTT GACAACAACA TGGAGTTTGC ACAGATCAAC 
GGAATTCACA AGTTTCAGGA GAACCTGAGT GCTTTCAAAT ATGAGGAGAA GATCAACATC 
TTGTTGCTGC CACTGCACAC AGGCTCCAAT GGACTGAACA TCATTGAAGC AACACACGTG 
CTACTGGTGG AGCCCATCCT AAACCCCGCC CACGAGCTGC AGGCCATAGG AAGAGTGCAT 
CGGATTGGCC AGACCAAACC CACATTTGTT CATCGGTTTC TGATTAAGTC CACCATTGAG 
GAACGGATGC AAGCCATGCT GAAAACAGCA GAAAAGAGTC ACAGTTCCAC GTCTATGAAG 
CATTCGGAGG CCTCTGTGTT TACAGTGGCA GACCTGGCGG ATCTGTTCAC CGAGGACAGC 
GAGACTCTTG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200358.1
CDS	142..5076
Translation	MSSRRKRAPPVRVDEDAKRRLDWNMHEDRKNEGACEDILESVPAPSDSCLPVPVLEQGFISSAGTEEEPSEERPSCSTQDSPPDTIELNVMPISVLDQGWNALIGEFNLHPKIPIEVADGSFCLQQTGDTLSMTLNTLEMEDGRGRSDPLCSVPVACSLGGLLLEDLDWLQKRKVIKLCHASGGVPVKLKVFLIESGLGRPEFLSEGNGRIKKASQLLQKLMELFYDFIIPEVMENEEEACDTDLERQNVEELYDHVRHLHQTEAAEQSFHVQHPALIPILRPYQSQAVNWMLRREKYKSSSSPEQRLHFLWREMVTICGKKLFYNPYTGCLIREFPLAGVDWPGGILADEMGLGKTVEVLALILCNTRQNLEHGVLTLPVGRSVNYFVPPPPLERQRIIKHEPRPKEKIIYPAVREMLLTAIKEMKVGKGASINAIFAYLRTMYKYDTLKNRNHIKKILTKLISENIVEQIKGRGLAGSFKLGKNYKEKKKKPVTARTKSADQPAPRRTSRRVSAPDKDSSLTSYTTTASDSASEDSMNPPLLTEELKSDPDSLLDPENPLQKKPVDITDDKEIPDKPQLQITEDVHIESNKPDLMQTSKPEVETPTRASVVPFNTPDYRFECICGELGLVDYKPRVQCLNCLLWQHAECVNYKEENLETTPFYCPHCLVAMTPVSTGATLIISPSSICHQWVDEINRHIHTSSLRVLVYQGVKRHGFIQPHILAEQDVVITTYDVLRTELNYVDIPHSNSKDGRRFRNQKRYMAIPSPLVAVEWWRVCLDEAQMVECTTAKAAEMALRLTSVNRWCVSGTPVQRGLEDLYGLVLFLGVDPYWVKYWWDQLLYRPYRHGNTAPLYNVIGQLLWRSAKKDVIDQIQIPPQTEEIHWLNFSPVERHFYHRQHEVCSQDALVKLRKISDWSLKLGSLDRRTVTTILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLKSLQKKCRVECEEAHRQLVCALNGLAGIHIIRSEFVDAAEMYREVLRSSEEHKARLKTDSLQRLHATHNLMELLSANHPGIPPTLRDDSLKEEAEQLKQHYMAKVNSEVSEAHQNLQPVLQHIKEQRRKVNLRSPWWLDVIQQAMQYSMDDDLVSRIQNELTCSYKQQANKLSMADKFRDARGLQFLLSTQMDDLMKSQKTVQDAVKKLEGPPSQQVIEEATLCHLRPVRLPLNNCVFCKADELFTDYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRLPTTSRGLWAASETERALKAILSFAKVRRLESGLIEEGNSFMELFESWKKEYKLLHEYWMSLRDHVSAIDELGMATERLRVRLPDEPKPKVLHIIEPHEVEQNRIKLLNDRAVAKSQLQKKLGQFLYLTNLEKSQDKSTGGLNPEPCPICARPLGQEWAVLTCGHCFCNECIAIIVEQYSIGNRRRAIKCAICRQTTSHAEISYVFTTQNNHQGQDIPVKGSHSTKVEAVVRVLKKVQMTDPGAKSLVFSTWQGVLDIIAKALFDNNMEFAQINGIHKFQENLSAFKYEEKINILLLPLHTGSNGLNIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTFVHRFLIKSTIEERMQAMLKTAEKSHSSTSMKHSEASVFTVADLADLFTEDSETLE

Target ORF information:

RefSeq Version	XM_003200310.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200310.5

ORF Insert Sequence:

ATGAGCAGCA GGAGGAAACG TGCGCCGCCA GTGCGCGTGG ATGAGGACGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGAAGA CATTCTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATTGA ACTGAATGTA ATGCCAATTT CTGTTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGGGA GGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAAGC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCATCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTAAG 
TCTGCTGACC AGCCCGCTCC CAGGAGAACA TCTCGTCGAG TTTCTGCTCC GGATAAAGAT 
TCCTCACTGA CCTCCTATAC AACAACAGCA TCAGATTCAG CATCGGAAGA CAGTATGAAT 
CCTCCTCTTC TCACAGAAGA GCTGAAATCT GATCCTGATT CACTTCTGGA CCCAGAAAAT 
CCCCTGCAGA AAAAACCTGT AGACATAACA GATGATAAAG AAATACCCGA CAAACCCCAA 
CTGCAAATAA CAGAAGACGT CCATATTGAA TCTAACAAAC CAGATCTTAT GCAAACCTCA 
AAACCTGAAG TCGAAACGCC AACCAGAGCG TCTGTGGTCC CGTTTAATAC TCCAGACTAC 
CGCTTTGAGT GCATCTGTGG AGAGCTGGGC CTGGTGGACT ATAAGCCCCG GGTTCAGTGT 
CTGAACTGTC TGTTATGGCA GCATGCCGAG TGTGTCAACT ATAAGGAGGA GAATCTGGAA 
ACGACGCCAT TTTATTGTCC TCACTGTCTG GTGGCCATGA CACCAGTGTC CACTGGAGCG 
ACTCTTATCA TCTCACCCAG CTCAATTTGT CATCAGTGGG TGGATGAAAT CAACCGGCAC 
ATTCACACCT CCTCCCTCAG AGTGCTGGTG TATCAGGGCG TGAAGAGACA CGGTTTTATC 
CAGCCTCACA TTCTGGCCGA GCAGGATGTG GTCATCACCA CTTATGACGT TCTGCGCACT 
GAACTCAATT ATGTGGATAT TCCTCACAGC AATAGTAAAG ATGGCCGTCG ATTCCGCAAC 
CAGAAGCGCT ACATGGCCAT ACCGAGCCCT CTAGTGGCTG TGGAGTGGTG GCGCGTTTGT 
CTAGATGAAG CTCAGATGGT GGAGTGCACC ACAGCCAAGG CTGCAGAAAT GGCTTTGCGT 
CTCACATCTG TTAATCGCTG GTGTGTGAGT GGAACACCTG TGCAGAGAGG TTTGGAGGAC 
CTGTATGGTC TCGTCCTGTT CTTGGGAGTT GATCCCTACT GGGTGAAGTA CTGGTGGGAT 
CAGTTACTCT ATCGCCCGTA CCGTCACGGC AATACAGCAC CGCTCTACAA CGTCATTGGT 
CAACTACTAT GGCGATCTGC CAAAAAAGAC GTCATTGATC AGATCCAAAT CCCCCCTCAA 
ACAGAGGAGA TCCATTGGCT TAACTTCTCC CCCGTGGAAA GACACTTTTA CCACCGTCAG 
CATGAGGTTT GCTCCCAGGA TGCTTTAGTG AAGCTAAGGA AGATCTCAGA TTGGTCACTG 
AAGCTAGGAA GTTTGGACCG TCGTACCGTC ACTACCATAT TATACCCACT TTTGCGTCTG 
CGCCAGGCCT GCTGCCACCC GCAGGCCGTC CGAGGAGAAT TTCTACCCCT TCAAAAAAGC 
ACAATGACAA TGGAGGAGTT GTTGAAGTCG CTGCAGAAGA AATGTCGTGT GGAGTGTGAA 
GAAGCGCACA GGCAGCTGGT CTGTGCTCTC AACGGTCTGG CTGGAATCCA CATCATTAGA 
AGTGAGTTTG TAGATGCAGC GGAGATGTAC AGGGAAGTGT TGCGATCATC TGAAGAACAC 
AAAGCAAGGC TGAAAACTGA TTCCCTTCAG AGGCTTCATG CAACCCACAA TCTAATGGAA 
CTTCTAAGTG CAAACCACCC TGGGATTCCT CCAACCCTGA GAGATGACAG TCTCAAAGAA 
GAGGCCGAGC AGCTGAAGCA GCATTACATG GCCAAAGTGA ACTCTGAGGT TTCTGAAGCA 
CATCAAAACC TACAGCCTGT TCTTCAGCAC ATTAAAGAAC AAAGACGCAA GGTAAACCTT 
CGCTCCCCCT GGTGGCTGGA TGTCATACAG CAGGCCATGC AGTACTCCAT GGATGATGAT 
CTTGTCAGCC GCATTCAAAA TGAGCTTACA TGCAGTTATA AACAACAAGC CAACAAGCTC 
TCAATGGCAG ACAAATTTCG AGACGCTCGG GGTCTTCAGT TTCTGCTCAG CACTCAAATG 
GATGATTTGA TGAAGTCACA GAAAACAGTC CAGGATGCTG TGAAGAAGCT GGAAGGGCCG 
CCGTCTCAAC AAGTCATCGA GGAAGCAACA CTTTGCCACC TGCGACCTGT TCGTCTGCCA 
CTGAACAACT GTGTGTTTTG CAAAGCAGAT GAACTTTTTA CAGACTACGA GTCGAAGCTT 
TTCTCGCACA CAGTAAAAGG GCAGACGGCT ATCTTTGAGG AGATGATTGA AGACGAGGAA 
GGGCTGGTAG ATGACAGGCT TCCCACCACC AGTAGAGGAT TATGGGCCGC CAGTGAGACC 
GAACGAGCTC TTAAAGCCAT CTTGTCCTTC GCTAAAGTCC GCCGCTTGGA GTCTGGCCTC 
ATCGAGGAGG GAAACTCCTT CATGGAGCTC TTCGAGTCCT GGAAGAAAGA ATACAAGCTT 
CTGCATGAGT ACTGGATGTC TCTGCGGGAT CATGTATCTG CTATAGATGA GCTGGGAATG 
GCCACCGAGA GGCTGCGTGT GCGCTTACCT GATGAGCCCA AACCCAAAGT CCTGCATATT 
ATTGAGCCTC ACGAGGTGGA GCAGAACAGG ATAAAGCTGC TCAATGACAG GGCAGTGGCC 
AAATCACAGC TGCAAAAAAA GCTTGGGCAG TTTCTCTATC TCACAAACCT GGAGAAGTCT 
CAGGATAAGT CCACTGGTGG TCTGAATCCA GAACCATGTC CAATCTGTGC TCGTCCGCTG 
GGTCAGGAGT GGGCCGTACT GACCTGTGGA CACTGTTTCT GTAACGAGTG CATCGCCATC 
ATTGTGGAGC AATACAGCAT CGGCAATCGA CGGAGAGCTA TAAAGTGTGC CATATGCCGA 
CAAACCACTT CACACGCAGA GATCTCATAT GTGTTCACCA CTCAAAACAA CCACCAGGGG 
CAGGACATCC CTGTGAAGGG TAGCCACTCC ACGAAGGTCG AGGCTGTGGT TCGAGTCCTA 
AAAAAAGTCC AGATGACCGA CCCGGGAGCC AAGTCTCTTG TGTTCTCCAC GTGGCAGGGC 
GTCCTGGACA TCATCGCCAA AGCCCTGTTT GACAACAACA TGGAGTTTGC ACAGATCAAC 
GGAATTCACA AGTTTCAGGA GAACCTGAGT GCTTTCAAAT ATGAGGAGAA GATCAACATC 
TTGTTGCTGC CACTGCACAC AGGCTCCAAT GGACTGAACA TCATTGAAGC AACACACGTG 
CTACTGGTGG AGCCCATCCT AAACCCCGCC CACGAGCTGC AGGCCATAGG AAGAGTGCAT 
CGGATTGGCC AGACCAAACC CACATTTGTT CATCGGTTTC TGATTAAGTC CACCATTGAG 
GAACGGATGC AAGCCATGCT GAAAACAGCA GAAAAGAGTC ACAGTTCCAC GTCTATGAAG 
CATTCGGAGG CCTCTGTGTT TACAGTGGCA GACCTGGCGG ATCTGTTCAC CGAGGACAGC 
GAGACTCTTG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shprh ( XM_009292830.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291105.1 Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X2, mRNA.

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CloneID	ODa43299
Clone ID Related Accession (Same CDS sequence)	XM_009292830.3 , XM_009292830.2
Accession Version	XM_009292830.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase SHPRH isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292830.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA GGAGGAAACG TGCGCCGCCA GTGCGCGTGG ATGAGGACGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGAAGA CATTCTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATTGA ACTGAATGTA ATGCCAATTT CTGTTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGGGA GGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAAGC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCATCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTTCT 
GCTGACCAGC CCGCTCCCAG GAGAACATCT CGTCGAGTTT CTGCTCCGGA TAAAGATTCC 
TCACTGACCT CCTATACAAC AACAGCATCA GATTCAGCAT CGGAAGACAG TATGAATCCT 
CCTCTTCTCA CAGAAGAGCT GAAATCTGAT CCTGATTCAC TTCTGGACCC AGAAAATCCC 
CTGCAGAAAA AACCTGTAGA CATAACAGAT GATAAAGAAA TACCCGACAA ACCCCAACTG 
CAAATAACAG AAGACGTCCA TATTGAATCT AACAAACCAG ATCTTATGCA AACCTCAAAA 
CCTGAAGTCG AAACGCCAAC CAGAGCGTCT GTGGTCCCGT TTAATACTCC AGACTACCGC 
TTTGAGTGCA TCTGTGGAGA GCTGGGCCTG GTGGACTATA AGCCCCGGGT TCAGTGTCTG 
AACTGTCTGT TATGGCAGCA TGCCGAGTGT GTCAACTATA AGGAGGAGAA TCTGGAAACG 
ACGCCATTTT ATTGTCCTCA CTGTCTGGTG GCCATGACAC CAGTGTCCAC TGGAGCGACT 
CTTATCATCT CACCCAGCTC AATTTGTCAT CAGTGGGTGG ATGAAATCAA CCGGCACATT 
CACACCTCCT CCCTCAGAGT GCTGGTGTAT CAGGGCGTGA AGAGACACGG TTTTATCCAG 
CCTCACATTC TGGCCGAGCA GGATGTGGTC ATCACCACTT ATGACGTTCT GCGCACTGAA 
CTCAATTATG TGGATATTCC TCACAGCAAT AGTAAAGATG GCCGTCGATT CCGCAACCAG 
AAGCGCTACA TGGCCATACC GAGCCCTCTA GTGGCTGTGG AGTGGTGGCG CGTTTGTCTA 
GATGAAGCTC AGATGGTGGA GTGCACCACA GCCAAGGCTG CAGAAATGGC TTTGCGTCTC 
ACATCTGTTA ATCGCTGGTG TGTGAGTGGA ACACCTGTGC AGAGAGGTTT GGAGGACCTG 
TATGGTCTCG TCCTGTTCTT GGGAGTTGAT CCCTACTGGG TGAAGTACTG GTGGGATCAG 
TTACTCTATC GCCCGTACCG TCACGGCAAT ACAGCACCGC TCTACAACGT CATTGGTCAA 
CTACTATGGC GATCTGCCAA AAAAGACGTC ATTGATCAGA TCCAAATCCC CCCTCAAACA 
GAGGAGATCC ATTGGCTTAA CTTCTCCCCC GTGGAAAGAC ACTTTTACCA CCGTCAGCAT 
GAGGTTTGCT CCCAGGATGC TTTAGTGAAG CTAAGGAAGA TCTCAGATTG GTCACTGAAG 
CTAGGAAGTT TGGACCGTCG TACCGTCACT ACCATATTAT ACCCACTTTT GCGTCTGCGC 
CAGGCCTGCT GCCACCCGCA GGCCGTCCGA GGAGAATTTC TACCCCTTCA AAAAAGCACA 
ATGACAATGG AGGAGTTGTT GAAGTCGCTG CAGAAGAAAT GTCGTGTGGA GTGTGAAGAA 
GCGCACAGGC AGCTGGTCTG TGCTCTCAAC GGTCTGGCTG GAATCCACAT CATTAGAAGT 
GAGTTTGTAG ATGCAGCGGA GATGTACAGG GAAGTGTTGC GATCATCTGA AGAACACAAA 
GCAAGGCTGA AAACTGATTC CCTTCAGAGG CTTCATGCAA CCCACAATCT AATGGAACTT 
CTAAGTGCAA ACCACCCTGG GATTCCTCCA ACCCTGAGAG ATGACAGTCT CAAAGAAGAG 
GCCGAGCAGC TGAAGCAGCA TTACATGGCC AAAGTGAACT CTGAGGTTTC TGAAGCACAT 
CAAAACCTAC AGCCTGTTCT TCAGCACATT AAAGAACAAA GACGCAAGGT AAACCTTCGC 
TCCCCCTGGT GGCTGGATGT CATACAGCAG GCCATGCAGT ACTCCATGGA TGATGATCTT 
GTCAGCCGCA TTCAAAATGA GCTTACATGC AGTTATAAAC AACAAGCCAA CAAGCTCTCA 
ATGGCAGACA AATTTCGAGA CGCTCGGGGT CTTCAGTTTC TGCTCAGCAC TCAAATGGAT 
GATTTGATGA AGTCACAGAA AACAGTCCAG GATGCTGTGA AGAAGCTGGA AGGGCCGCCG 
TCTCAACAAG TCATCGAGGA AGCAACACTT TGCCACCTGC GACCTGTTCG TCTGCCACTG 
AACAACTGTG TGTTTTGCAA AGCAGATGAA CTTTTTACAG ACTACGAGTC GAAGCTTTTC 
TCGCACACAG TAAAAGGGCA GACGGCTATC TTTGAGGAGA TGATTGAAGA CGAGGAAGGG 
CTGGTAGATG ACAGGCTTCC CACCACCAGT AGAGGATTAT GGGCCGCCAG TGAGACCGAA 
CGAGCTCTTA AAGCCATCTT GTCCTTCGCT AAAGTCCGCC GCTTGGAGTC TGGCCTCATC 
GAGGAGGGAA ACTCCTTCAT GGAGCTCTTC GAGTCCTGGA AGAAAGAATA CAAGCTTCTG 
CATGAGTACT GGATGTCTCT GCGGGATCAT GTATCTGCTA TAGATGAGCT GGGAATGGCC 
ACCGAGAGGC TGCGTGTGCG CTTACCTGAT GAGCCCAAAC CCAAAGTCCT GCATATTATT 
GAGCCTCACG AGGTGGAGCA GAACAGGATA AAGCTGCTCA ATGACAGGGC AGTGGCCAAA 
TCACAGCTGC AAAAAAAGCT TGGGCAGTTT CTCTATCTCA CAAACCTGGA GAAGTCTCAG 
GATAAGTCCA CTGGTGGTCT GAATCCAGAA CCATGTCCAA TCTGTGCTCG TCCGCTGGGT 
CAGGAGTGGG CCGTACTGAC CTGTGGACAC TGTTTCTGTA ACGAGTGCAT CGCCATCATT 
GTGGAGCAAT ACAGCATCGG CAATCGACGG AGAGCTATAA AGTGTGCCAT ATGCCGACAA 
ACCACTTCAC ACGCAGAGAT CTCATATGTG TTCACCACTC AAAACAACCA CCAGGGGCAG 
GACATCCCTG TGAAGGGTAG CCACTCCACG AAGGTCGAGG CTGTGGTTCG AGTCCTAAAA 
AAAGTCCAGA TGACCGACCC GGGAGCCAAG TCTCTTGTGT TCTCCACGTG GCAGGGCGTC 
CTGGACATCA TCGCCAAAGC CCTGTTTGAC AACAACATGG AGTTTGCACA GATCAACGGA 
ATTCACAAGT TTCAGGAGAA CCTGAGTGCT TTCAAATATG AGGAGAAGAT CAACATCTTG 
TTGCTGCCAC TGCACACAGG CTCCAATGGA CTGAACATCA TTGAAGCAAC ACACGTGCTA 
CTGGTGGAGC CCATCCTAAA CCCCGCCCAC GAGCTGCAGG CCATAGGAAG AGTGCATCGG 
ATTGGCCAGA CCAAACCCAC ATTTGTTCAT CGGTTTCTGA TTAAGTCCAC CATTGAGGAA 
CGGATGCAAG CCATGCTGAA AACAGCAGAA AAGAGTCACA GTTCCACGTC TATGAAGCAT 
TCGGAGGCCT CTGTGTTTAC AGTGGCAGAC CTGGCGGATC TGTTCACCGA GGACAGCGAG 
ACTCTTGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291105.1
CDS	142..5073
Translation	MSSRRKRAPPVRVDEDAKRRLDWNMHEDRKNEGACEDILESVPAPSDSCLPVPVLEQGFISSAGTEEEPSEERPSCSTQDSPPDTIELNVMPISVLDQGWNALIGEFNLHPKIPIEVADGSFCLQQTGDTLSMTLNTLEMEDGRGRSDPLCSVPVACSLGGLLLEDLDWLQKRKVIKLCHASGGVPVKLKVFLIESGLGRPEFLSEGNGRIKKASQLLQKLMELFYDFIIPEVMENEEEACDTDLERQNVEELYDHVRHLHQTEAAEQSFHVQHPALIPILRPYQSQAVNWMLRREKYKSSSSPEQRLHFLWREMVTICGKKLFYNPYTGCLIREFPLAGVDWPGGILADEMGLGKTVEVLALILCNTRQNLEHGVLTLPVGRSVNYFVPPPPLERQRIIKHEPRPKEKIIYPAVREMLLTAIKEMKVGKGASINAIFAYLRTMYKYDTLKNRNHIKKILTKLISENIVEQIKGRGLAGSFKLGKNYKEKKKKPVTARTSADQPAPRRTSRRVSAPDKDSSLTSYTTTASDSASEDSMNPPLLTEELKSDPDSLLDPENPLQKKPVDITDDKEIPDKPQLQITEDVHIESNKPDLMQTSKPEVETPTRASVVPFNTPDYRFECICGELGLVDYKPRVQCLNCLLWQHAECVNYKEENLETTPFYCPHCLVAMTPVSTGATLIISPSSICHQWVDEINRHIHTSSLRVLVYQGVKRHGFIQPHILAEQDVVITTYDVLRTELNYVDIPHSNSKDGRRFRNQKRYMAIPSPLVAVEWWRVCLDEAQMVECTTAKAAEMALRLTSVNRWCVSGTPVQRGLEDLYGLVLFLGVDPYWVKYWWDQLLYRPYRHGNTAPLYNVIGQLLWRSAKKDVIDQIQIPPQTEEIHWLNFSPVERHFYHRQHEVCSQDALVKLRKISDWSLKLGSLDRRTVTTILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLKSLQKKCRVECEEAHRQLVCALNGLAGIHIIRSEFVDAAEMYREVLRSSEEHKARLKTDSLQRLHATHNLMELLSANHPGIPPTLRDDSLKEEAEQLKQHYMAKVNSEVSEAHQNLQPVLQHIKEQRRKVNLRSPWWLDVIQQAMQYSMDDDLVSRIQNELTCSYKQQANKLSMADKFRDARGLQFLLSTQMDDLMKSQKTVQDAVKKLEGPPSQQVIEEATLCHLRPVRLPLNNCVFCKADELFTDYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRLPTTSRGLWAASETERALKAILSFAKVRRLESGLIEEGNSFMELFESWKKEYKLLHEYWMSLRDHVSAIDELGMATERLRVRLPDEPKPKVLHIIEPHEVEQNRIKLLNDRAVAKSQLQKKLGQFLYLTNLEKSQDKSTGGLNPEPCPICARPLGQEWAVLTCGHCFCNECIAIIVEQYSIGNRRRAIKCAICRQTTSHAEISYVFTTQNNHQGQDIPVKGSHSTKVEAVVRVLKKVQMTDPGAKSLVFSTWQGVLDIIAKALFDNNMEFAQINGIHKFQENLSAFKYEEKINILLLPLHTGSNGLNIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTFVHRFLIKSTIEERMQAMLKTAEKSHSSTSMKHSEASVFTVADLADLFTEDSETLE

Target ORF information:

RefSeq Version	XM_009292830.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292830.3

ORF Insert Sequence:

ATGAGCAGCA GGAGGAAACG TGCGCCGCCA GTGCGCGTGG ATGAGGACGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGAAGA CATTCTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATTGA ACTGAATGTA ATGCCAATTT CTGTTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGGGA GGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAAGC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCATCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTTCT 
GCTGACCAGC CCGCTCCCAG GAGAACATCT CGTCGAGTTT CTGCTCCGGA TAAAGATTCC 
TCACTGACCT CCTATACAAC AACAGCATCA GATTCAGCAT CGGAAGACAG TATGAATCCT 
CCTCTTCTCA CAGAAGAGCT GAAATCTGAT CCTGATTCAC TTCTGGACCC AGAAAATCCC 
CTGCAGAAAA AACCTGTAGA CATAACAGAT GATAAAGAAA TACCCGACAA ACCCCAACTG 
CAAATAACAG AAGACGTCCA TATTGAATCT AACAAACCAG ATCTTATGCA AACCTCAAAA 
CCTGAAGTCG AAACGCCAAC CAGAGCGTCT GTGGTCCCGT TTAATACTCC AGACTACCGC 
TTTGAGTGCA TCTGTGGAGA GCTGGGCCTG GTGGACTATA AGCCCCGGGT TCAGTGTCTG 
AACTGTCTGT TATGGCAGCA TGCCGAGTGT GTCAACTATA AGGAGGAGAA TCTGGAAACG 
ACGCCATTTT ATTGTCCTCA CTGTCTGGTG GCCATGACAC CAGTGTCCAC TGGAGCGACT 
CTTATCATCT CACCCAGCTC AATTTGTCAT CAGTGGGTGG ATGAAATCAA CCGGCACATT 
CACACCTCCT CCCTCAGAGT GCTGGTGTAT CAGGGCGTGA AGAGACACGG TTTTATCCAG 
CCTCACATTC TGGCCGAGCA GGATGTGGTC ATCACCACTT ATGACGTTCT GCGCACTGAA 
CTCAATTATG TGGATATTCC TCACAGCAAT AGTAAAGATG GCCGTCGATT CCGCAACCAG 
AAGCGCTACA TGGCCATACC GAGCCCTCTA GTGGCTGTGG AGTGGTGGCG CGTTTGTCTA 
GATGAAGCTC AGATGGTGGA GTGCACCACA GCCAAGGCTG CAGAAATGGC TTTGCGTCTC 
ACATCTGTTA ATCGCTGGTG TGTGAGTGGA ACACCTGTGC AGAGAGGTTT GGAGGACCTG 
TATGGTCTCG TCCTGTTCTT GGGAGTTGAT CCCTACTGGG TGAAGTACTG GTGGGATCAG 
TTACTCTATC GCCCGTACCG TCACGGCAAT ACAGCACCGC TCTACAACGT CATTGGTCAA 
CTACTATGGC GATCTGCCAA AAAAGACGTC ATTGATCAGA TCCAAATCCC CCCTCAAACA 
GAGGAGATCC ATTGGCTTAA CTTCTCCCCC GTGGAAAGAC ACTTTTACCA CCGTCAGCAT 
GAGGTTTGCT CCCAGGATGC TTTAGTGAAG CTAAGGAAGA TCTCAGATTG GTCACTGAAG 
CTAGGAAGTT TGGACCGTCG TACCGTCACT ACCATATTAT ACCCACTTTT GCGTCTGCGC 
CAGGCCTGCT GCCACCCGCA GGCCGTCCGA GGAGAATTTC TACCCCTTCA AAAAAGCACA 
ATGACAATGG AGGAGTTGTT GAAGTCGCTG CAGAAGAAAT GTCGTGTGGA GTGTGAAGAA 
GCGCACAGGC AGCTGGTCTG TGCTCTCAAC GGTCTGGCTG GAATCCACAT CATTAGAAGT 
GAGTTTGTAG ATGCAGCGGA GATGTACAGG GAAGTGTTGC GATCATCTGA AGAACACAAA 
GCAAGGCTGA AAACTGATTC CCTTCAGAGG CTTCATGCAA CCCACAATCT AATGGAACTT 
CTAAGTGCAA ACCACCCTGG GATTCCTCCA ACCCTGAGAG ATGACAGTCT CAAAGAAGAG 
GCCGAGCAGC TGAAGCAGCA TTACATGGCC AAAGTGAACT CTGAGGTTTC TGAAGCACAT 
CAAAACCTAC AGCCTGTTCT TCAGCACATT AAAGAACAAA GACGCAAGGT AAACCTTCGC 
TCCCCCTGGT GGCTGGATGT CATACAGCAG GCCATGCAGT ACTCCATGGA TGATGATCTT 
GTCAGCCGCA TTCAAAATGA GCTTACATGC AGTTATAAAC AACAAGCCAA CAAGCTCTCA 
ATGGCAGACA AATTTCGAGA CGCTCGGGGT CTTCAGTTTC TGCTCAGCAC TCAAATGGAT 
GATTTGATGA AGTCACAGAA AACAGTCCAG GATGCTGTGA AGAAGCTGGA AGGGCCGCCG 
TCTCAACAAG TCATCGAGGA AGCAACACTT TGCCACCTGC GACCTGTTCG TCTGCCACTG 
AACAACTGTG TGTTTTGCAA AGCAGATGAA CTTTTTACAG ACTACGAGTC GAAGCTTTTC 
TCGCACACAG TAAAAGGGCA GACGGCTATC TTTGAGGAGA TGATTGAAGA CGAGGAAGGG 
CTGGTAGATG ACAGGCTTCC CACCACCAGT AGAGGATTAT GGGCCGCCAG TGAGACCGAA 
CGAGCTCTTA AAGCCATCTT GTCCTTCGCT AAAGTCCGCC GCTTGGAGTC TGGCCTCATC 
GAGGAGGGAA ACTCCTTCAT GGAGCTCTTC GAGTCCTGGA AGAAAGAATA CAAGCTTCTG 
CATGAGTACT GGATGTCTCT GCGGGATCAT GTATCTGCTA TAGATGAGCT GGGAATGGCC 
ACCGAGAGGC TGCGTGTGCG CTTACCTGAT GAGCCCAAAC CCAAAGTCCT GCATATTATT 
GAGCCTCACG AGGTGGAGCA GAACAGGATA AAGCTGCTCA ATGACAGGGC AGTGGCCAAA 
TCACAGCTGC AAAAAAAGCT TGGGCAGTTT CTCTATCTCA CAAACCTGGA GAAGTCTCAG 
GATAAGTCCA CTGGTGGTCT GAATCCAGAA CCATGTCCAA TCTGTGCTCG TCCGCTGGGT 
CAGGAGTGGG CCGTACTGAC CTGTGGACAC TGTTTCTGTA ACGAGTGCAT CGCCATCATT 
GTGGAGCAAT ACAGCATCGG CAATCGACGG AGAGCTATAA AGTGTGCCAT ATGCCGACAA 
ACCACTTCAC ACGCAGAGAT CTCATATGTG TTCACCACTC AAAACAACCA CCAGGGGCAG 
GACATCCCTG TGAAGGGTAG CCACTCCACG AAGGTCGAGG CTGTGGTTCG AGTCCTAAAA 
AAAGTCCAGA TGACCGACCC GGGAGCCAAG TCTCTTGTGT TCTCCACGTG GCAGGGCGTC 
CTGGACATCA TCGCCAAAGC CCTGTTTGAC AACAACATGG AGTTTGCACA GATCAACGGA 
ATTCACAAGT TTCAGGAGAA CCTGAGTGCT TTCAAATATG AGGAGAAGAT CAACATCTTG 
TTGCTGCCAC TGCACACAGG CTCCAATGGA CTGAACATCA TTGAAGCAAC ACACGTGCTA 
CTGGTGGAGC CCATCCTAAA CCCCGCCCAC GAGCTGCAGG CCATAGGAAG AGTGCATCGG 
ATTGGCCAGA CCAAACCCAC ATTTGTTCAT CGGTTTCTGA TTAAGTCCAC CATTGAGGAA 
CGGATGCAAG CCATGCTGAA AACAGCAGAA AAGAGTCACA GTTCCACGTC TATGAAGCAT 
TCGGAGGCCT CTGTGTTTAC AGTGGCAGAC CTGGCGGATC TGTTCACCGA GGACAGCGAG 
ACTCTTGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shprh ( XM_009292830.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291105.1 Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X2, mRNA.

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CloneID	ODa43299
Clone ID Related Accession (Same CDS sequence)	XM_009292830.3 , XM_009292830.2
Accession Version	XM_009292830.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase SHPRH isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292830.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA GGAGGAAACG TGCGCCGCCA GTGCGCGTGG ATGAGGACGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGAAGA CATTCTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATTGA ACTGAATGTA ATGCCAATTT CTGTTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGGGA GGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAAGC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCATCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTTCT 
GCTGACCAGC CCGCTCCCAG GAGAACATCT CGTCGAGTTT CTGCTCCGGA TAAAGATTCC 
TCACTGACCT CCTATACAAC AACAGCATCA GATTCAGCAT CGGAAGACAG TATGAATCCT 
CCTCTTCTCA CAGAAGAGCT GAAATCTGAT CCTGATTCAC TTCTGGACCC AGAAAATCCC 
CTGCAGAAAA AACCTGTAGA CATAACAGAT GATAAAGAAA TACCCGACAA ACCCCAACTG 
CAAATAACAG AAGACGTCCA TATTGAATCT AACAAACCAG ATCTTATGCA AACCTCAAAA 
CCTGAAGTCG AAACGCCAAC CAGAGCGTCT GTGGTCCCGT TTAATACTCC AGACTACCGC 
TTTGAGTGCA TCTGTGGAGA GCTGGGCCTG GTGGACTATA AGCCCCGGGT TCAGTGTCTG 
AACTGTCTGT TATGGCAGCA TGCCGAGTGT GTCAACTATA AGGAGGAGAA TCTGGAAACG 
ACGCCATTTT ATTGTCCTCA CTGTCTGGTG GCCATGACAC CAGTGTCCAC TGGAGCGACT 
CTTATCATCT CACCCAGCTC AATTTGTCAT CAGTGGGTGG ATGAAATCAA CCGGCACATT 
CACACCTCCT CCCTCAGAGT GCTGGTGTAT CAGGGCGTGA AGAGACACGG TTTTATCCAG 
CCTCACATTC TGGCCGAGCA GGATGTGGTC ATCACCACTT ATGACGTTCT GCGCACTGAA 
CTCAATTATG TGGATATTCC TCACAGCAAT AGTAAAGATG GCCGTCGATT CCGCAACCAG 
AAGCGCTACA TGGCCATACC GAGCCCTCTA GTGGCTGTGG AGTGGTGGCG CGTTTGTCTA 
GATGAAGCTC AGATGGTGGA GTGCACCACA GCCAAGGCTG CAGAAATGGC TTTGCGTCTC 
ACATCTGTTA ATCGCTGGTG TGTGAGTGGA ACACCTGTGC AGAGAGGTTT GGAGGACCTG 
TATGGTCTCG TCCTGTTCTT GGGAGTTGAT CCCTACTGGG TGAAGTACTG GTGGGATCAG 
TTACTCTATC GCCCGTACCG TCACGGCAAT ACAGCACCGC TCTACAACGT CATTGGTCAA 
CTACTATGGC GATCTGCCAA AAAAGACGTC ATTGATCAGA TCCAAATCCC CCCTCAAACA 
GAGGAGATCC ATTGGCTTAA CTTCTCCCCC GTGGAAAGAC ACTTTTACCA CCGTCAGCAT 
GAGGTTTGCT CCCAGGATGC TTTAGTGAAG CTAAGGAAGA TCTCAGATTG GTCACTGAAG 
CTAGGAAGTT TGGACCGTCG TACCGTCACT ACCATATTAT ACCCACTTTT GCGTCTGCGC 
CAGGCCTGCT GCCACCCGCA GGCCGTCCGA GGAGAATTTC TACCCCTTCA AAAAAGCACA 
ATGACAATGG AGGAGTTGTT GAAGTCGCTG CAGAAGAAAT GTCGTGTGGA GTGTGAAGAA 
GCGCACAGGC AGCTGGTCTG TGCTCTCAAC GGTCTGGCTG GAATCCACAT CATTAGAAGT 
GAGTTTGTAG ATGCAGCGGA GATGTACAGG GAAGTGTTGC GATCATCTGA AGAACACAAA 
GCAAGGCTGA AAACTGATTC CCTTCAGAGG CTTCATGCAA CCCACAATCT AATGGAACTT 
CTAAGTGCAA ACCACCCTGG GATTCCTCCA ACCCTGAGAG ATGACAGTCT CAAAGAAGAG 
GCCGAGCAGC TGAAGCAGCA TTACATGGCC AAAGTGAACT CTGAGGTTTC TGAAGCACAT 
CAAAACCTAC AGCCTGTTCT TCAGCACATT AAAGAACAAA GACGCAAGGT AAACCTTCGC 
TCCCCCTGGT GGCTGGATGT CATACAGCAG GCCATGCAGT ACTCCATGGA TGATGATCTT 
GTCAGCCGCA TTCAAAATGA GCTTACATGC AGTTATAAAC AACAAGCCAA CAAGCTCTCA 
ATGGCAGACA AATTTCGAGA CGCTCGGGGT CTTCAGTTTC TGCTCAGCAC TCAAATGGAT 
GATTTGATGA AGTCACAGAA AACAGTCCAG GATGCTGTGA AGAAGCTGGA AGGGCCGCCG 
TCTCAACAAG TCATCGAGGA AGCAACACTT TGCCACCTGC GACCTGTTCG TCTGCCACTG 
AACAACTGTG TGTTTTGCAA AGCAGATGAA CTTTTTACAG ACTACGAGTC GAAGCTTTTC 
TCGCACACAG TAAAAGGGCA GACGGCTATC TTTGAGGAGA TGATTGAAGA CGAGGAAGGG 
CTGGTAGATG ACAGGCTTCC CACCACCAGT AGAGGATTAT GGGCCGCCAG TGAGACCGAA 
CGAGCTCTTA AAGCCATCTT GTCCTTCGCT AAAGTCCGCC GCTTGGAGTC TGGCCTCATC 
GAGGAGGGAA ACTCCTTCAT GGAGCTCTTC GAGTCCTGGA AGAAAGAATA CAAGCTTCTG 
CATGAGTACT GGATGTCTCT GCGGGATCAT GTATCTGCTA TAGATGAGCT GGGAATGGCC 
ACCGAGAGGC TGCGTGTGCG CTTACCTGAT GAGCCCAAAC CCAAAGTCCT GCATATTATT 
GAGCCTCACG AGGTGGAGCA GAACAGGATA AAGCTGCTCA ATGACAGGGC AGTGGCCAAA 
TCACAGCTGC AAAAAAAGCT TGGGCAGTTT CTCTATCTCA CAAACCTGGA GAAGTCTCAG 
GATAAGTCCA CTGGTGGTCT GAATCCAGAA CCATGTCCAA TCTGTGCTCG TCCGCTGGGT 
CAGGAGTGGG CCGTACTGAC CTGTGGACAC TGTTTCTGTA ACGAGTGCAT CGCCATCATT 
GTGGAGCAAT ACAGCATCGG CAATCGACGG AGAGCTATAA AGTGTGCCAT ATGCCGACAA 
ACCACTTCAC ACGCAGAGAT CTCATATGTG TTCACCACTC AAAACAACCA CCAGGGGCAG 
GACATCCCTG TGAAGGGTAG CCACTCCACG AAGGTCGAGG CTGTGGTTCG AGTCCTAAAA 
AAAGTCCAGA TGACCGACCC GGGAGCCAAG TCTCTTGTGT TCTCCACGTG GCAGGGCGTC 
CTGGACATCA TCGCCAAAGC CCTGTTTGAC AACAACATGG AGTTTGCACA GATCAACGGA 
ATTCACAAGT TTCAGGAGAA CCTGAGTGCT TTCAAATATG AGGAGAAGAT CAACATCTTG 
TTGCTGCCAC TGCACACAGG CTCCAATGGA CTGAACATCA TTGAAGCAAC ACACGTGCTA 
CTGGTGGAGC CCATCCTAAA CCCCGCCCAC GAGCTGCAGG CCATAGGAAG AGTGCATCGG 
ATTGGCCAGA CCAAACCCAC ATTTGTTCAT CGGTTTCTGA TTAAGTCCAC CATTGAGGAA 
CGGATGCAAG CCATGCTGAA AACAGCAGAA AAGAGTCACA GTTCCACGTC TATGAAGCAT 
TCGGAGGCCT CTGTGTTTAC AGTGGCAGAC CTGGCGGATC TGTTCACCGA GGACAGCGAG 
ACTCTTGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291105.1
CDS	122..5053
Translation	MSSRRKRAPPVRVDEDAKRRLDWNMHEDRKNEGACEDILESVPAPSDSCLPVPVLEQGFISSAGTEEEPSEERPSCSTQDSPPDTIELNVMPISVLDQGWNALIGEFNLHPKIPIEVADGSFCLQQTGDTLSMTLNTLEMEDGRGRSDPLCSVPVACSLGGLLLEDLDWLQKRKVIKLCHASGGVPVKLKVFLIESGLGRPEFLSEGNGRIKKASQLLQKLMELFYDFIIPEVMENEEEACDTDLERQNVEELYDHVRHLHQTEAAEQSFHVQHPALIPILRPYQSQAVNWMLRREKYKSSSSPEQRLHFLWREMVTICGKKLFYNPYTGCLIREFPLAGVDWPGGILADEMGLGKTVEVLALILCNTRQNLEHGVLTLPVGRSVNYFVPPPPLERQRIIKHEPRPKEKIIYPAVREMLLTAIKEMKVGKGASINAIFAYLRTMYKYDTLKNRNHIKKILTKLISENIVEQIKGRGLAGSFKLGKNYKEKKKKPVTARTSADQPAPRRTSRRVSAPDKDSSLTSYTTTASDSASEDSMNPPLLTEELKSDPDSLLDPENPLQKKPVDITDDKEIPDKPQLQITEDVHIESNKPDLMQTSKPEVETPTRASVVPFNTPDYRFECICGELGLVDYKPRVQCLNCLLWQHAECVNYKEENLETTPFYCPHCLVAMTPVSTGATLIISPSSICHQWVDEINRHIHTSSLRVLVYQGVKRHGFIQPHILAEQDVVITTYDVLRTELNYVDIPHSNSKDGRRFRNQKRYMAIPSPLVAVEWWRVCLDEAQMVECTTAKAAEMALRLTSVNRWCVSGTPVQRGLEDLYGLVLFLGVDPYWVKYWWDQLLYRPYRHGNTAPLYNVIGQLLWRSAKKDVIDQIQIPPQTEEIHWLNFSPVERHFYHRQHEVCSQDALVKLRKISDWSLKLGSLDRRTVTTILYPLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLKSLQKKCRVECEEAHRQLVCALNGLAGIHIIRSEFVDAAEMYREVLRSSEEHKARLKTDSLQRLHATHNLMELLSANHPGIPPTLRDDSLKEEAEQLKQHYMAKVNSEVSEAHQNLQPVLQHIKEQRRKVNLRSPWWLDVIQQAMQYSMDDDLVSRIQNELTCSYKQQANKLSMADKFRDARGLQFLLSTQMDDLMKSQKTVQDAVKKLEGPPSQQVIEEATLCHLRPVRLPLNNCVFCKADELFTDYESKLFSHTVKGQTAIFEEMIEDEEGLVDDRLPTTSRGLWAASETERALKAILSFAKVRRLESGLIEEGNSFMELFESWKKEYKLLHEYWMSLRDHVSAIDELGMATERLRVRLPDEPKPKVLHIIEPHEVEQNRIKLLNDRAVAKSQLQKKLGQFLYLTNLEKSQDKSTGGLNPEPCPICARPLGQEWAVLTCGHCFCNECIAIIVEQYSIGNRRRAIKCAICRQTTSHAEISYVFTTQNNHQGQDIPVKGSHSTKVEAVVRVLKKVQMTDPGAKSLVFSTWQGVLDIIAKALFDNNMEFAQINGIHKFQENLSAFKYEEKINILLLPLHTGSNGLNIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTFVHRFLIKSTIEERMQAMLKTAEKSHSSTSMKHSEASVFTVADLADLFTEDSETLE

Target ORF information:

RefSeq Version	XM_009292830.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SNF2 histone linker PHD RING helicase (shprh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292830.2

ORF Insert Sequence:

ATGAGCAGCA GGAGGAAACG TGCGCCGCCA GTGCGCGTGG ATGAGGACGC CAAGAGGAGA 
CTGGACTGGA ATATGCATGA AGACCGCAAG AATGAAGGAG CTTGTGAAGA CATTCTGGAG 
TCCGTCCCAG CACCGTCTGA CAGCTGTCTG CCTGTACCAG TGCTTGAGCA AGGTTTTATA 
TCTTCTGCAG GCACAGAAGA AGAACCATCA GAAGAGCGTC CAAGCTGCTC CACACAGGAT 
TCTCCTCCAG ACACCATTGA ACTGAATGTA ATGCCAATTT CTGTTCTGGA TCAAGGGTGG 
AACGCACTGA TCGGAGAGTT TAACTTGCAT CCAAAGATTC CTATTGAGGT TGCAGATGGT 
TCATTCTGCC TGCAGCAAAC AGGAGACACA CTGAGCATGA CTCTGAACAC TCTTGAGATG 
GAGGATGGGA GGGGTAGAAG TGATCCGCTC TGTTCGGTGC CTGTCGCATG TTCACTTGGT 
GGACTGTTGT TGGAGGATCT GGATTGGCTG CAGAAGAGGA AGGTCATTAA ATTGTGTCAT 
GCATCAGGAG GTGTGCCGGT GAAGCTGAAA GTTTTTCTTA TTGAATCAGG ATTGGGCAGA 
CCGGAATTCC TCAGTGAAGG AAACGGACGG ATTAAAAAAG CCAGTCAGCT TCTGCAGAAA 
TTAATGGAAT TGTTTTACGA CTTCATCATC CCTGAGGTGA TGGAGAATGA AGAGGAGGCG 
TGTGACACTG ATCTGGAGCG TCAGAACGTG GAGGAGCTGT ACGATCACGT CAGACACCTC 
CACCAGACCG AAGCAGCCGA GCAGAGCTTC CACGTCCAGC ACCCGGCTCT CATCCCCATC 
CTGAGACCCT ACCAGAGTCA GGCCGTCAAC TGGATGCTAA GGAGGGAGAA ATACAAAAGC 
AGCAGCTCTC CAGAGCAAAG GTTGCATTTT CTTTGGAGGG AAATGGTCAC GATTTGCGGG 
AAAAAACTCT TTTACAATCC CTACACTGGA TGCCTGATTC GAGAGTTTCC TCTGGCTGGA 
GTGGACTGGC CCGGCGGGAT CCTTGCTGAT GAGATGGGAC TTGGGAAAAC TGTGGAAGTA 
CTGGCTCTGA TTCTGTGTAA CACTCGGCAA AACCTGGAGC ACGGGGTCCT CACTTTACCT 
GTGGGTCGGT CTGTGAATTA CTTTGTTCCT CCTCCACCAT TAGAGAGACA AAGAATCATA 
AAGCATGAGC CGAGACCCAA AGAGAAGATC ATATATCCAG CTGTTCGTGA GATGCTGTTA 
ACTGCTATTA AGGAAATGAA GGTTGGAAAA GGAGCATCGA TTAATGCAAT TTTTGCTTAT 
CTGCGGACCA TGTACAAGTA CGACACCCTG AAGAACCGCA ACCACATCAA GAAAATCCTG 
ACCAAGCTCA TTAGCGAGAA CATTGTGGAG CAGATCAAAG GACGAGGCTT AGCGGGATCC 
TTCAAACTGG GGAAGAACTA CAAAGAGAAG AAGAAGAAGC CTGTCACTGC ACGAACTTCT 
GCTGACCAGC CCGCTCCCAG GAGAACATCT CGTCGAGTTT CTGCTCCGGA TAAAGATTCC 
TCACTGACCT CCTATACAAC AACAGCATCA GATTCAGCAT CGGAAGACAG TATGAATCCT 
CCTCTTCTCA CAGAAGAGCT GAAATCTGAT CCTGATTCAC TTCTGGACCC AGAAAATCCC 
CTGCAGAAAA AACCTGTAGA CATAACAGAT GATAAAGAAA TACCCGACAA ACCCCAACTG 
CAAATAACAG AAGACGTCCA TATTGAATCT AACAAACCAG ATCTTATGCA AACCTCAAAA 
CCTGAAGTCG AAACGCCAAC CAGAGCGTCT GTGGTCCCGT TTAATACTCC AGACTACCGC 
TTTGAGTGCA TCTGTGGAGA GCTGGGCCTG GTGGACTATA AGCCCCGGGT TCAGTGTCTG 
AACTGTCTGT TATGGCAGCA TGCCGAGTGT GTCAACTATA AGGAGGAGAA TCTGGAAACG 
ACGCCATTTT ATTGTCCTCA CTGTCTGGTG GCCATGACAC CAGTGTCCAC TGGAGCGACT 
CTTATCATCT CACCCAGCTC AATTTGTCAT CAGTGGGTGG ATGAAATCAA CCGGCACATT 
CACACCTCCT CCCTCAGAGT GCTGGTGTAT CAGGGCGTGA AGAGACACGG TTTTATCCAG 
CCTCACATTC TGGCCGAGCA GGATGTGGTC ATCACCACTT ATGACGTTCT GCGCACTGAA 
CTCAATTATG TGGATATTCC TCACAGCAAT AGTAAAGATG GCCGTCGATT CCGCAACCAG 
AAGCGCTACA TGGCCATACC GAGCCCTCTA GTGGCTGTGG AGTGGTGGCG CGTTTGTCTA 
GATGAAGCTC AGATGGTGGA GTGCACCACA GCCAAGGCTG CAGAAATGGC TTTGCGTCTC 
ACATCTGTTA ATCGCTGGTG TGTGAGTGGA ACACCTGTGC AGAGAGGTTT GGAGGACCTG 
TATGGTCTCG TCCTGTTCTT GGGAGTTGAT CCCTACTGGG TGAAGTACTG GTGGGATCAG 
TTACTCTATC GCCCGTACCG TCACGGCAAT ACAGCACCGC TCTACAACGT CATTGGTCAA 
CTACTATGGC GATCTGCCAA AAAAGACGTC ATTGATCAGA TCCAAATCCC CCCTCAAACA 
GAGGAGATCC ATTGGCTTAA CTTCTCCCCC GTGGAAAGAC ACTTTTACCA CCGTCAGCAT 
GAGGTTTGCT CCCAGGATGC TTTAGTGAAG CTAAGGAAGA TCTCAGATTG GTCACTGAAG 
CTAGGAAGTT TGGACCGTCG TACCGTCACT ACCATATTAT ACCCACTTTT GCGTCTGCGC 
CAGGCCTGCT GCCACCCGCA GGCCGTCCGA GGAGAATTTC TACCCCTTCA AAAAAGCACA 
ATGACAATGG AGGAGTTGTT GAAGTCGCTG CAGAAGAAAT GTCGTGTGGA GTGTGAAGAA 
GCGCACAGGC AGCTGGTCTG TGCTCTCAAC GGTCTGGCTG GAATCCACAT CATTAGAAGT 
GAGTTTGTAG ATGCAGCGGA GATGTACAGG GAAGTGTTGC GATCATCTGA AGAACACAAA 
GCAAGGCTGA AAACTGATTC CCTTCAGAGG CTTCATGCAA CCCACAATCT AATGGAACTT 
CTAAGTGCAA ACCACCCTGG GATTCCTCCA ACCCTGAGAG ATGACAGTCT CAAAGAAGAG 
GCCGAGCAGC TGAAGCAGCA TTACATGGCC AAAGTGAACT CTGAGGTTTC TGAAGCACAT 
CAAAACCTAC AGCCTGTTCT TCAGCACATT AAAGAACAAA GACGCAAGGT AAACCTTCGC 
TCCCCCTGGT GGCTGGATGT CATACAGCAG GCCATGCAGT ACTCCATGGA TGATGATCTT 
GTCAGCCGCA TTCAAAATGA GCTTACATGC AGTTATAAAC AACAAGCCAA CAAGCTCTCA 
ATGGCAGACA AATTTCGAGA CGCTCGGGGT CTTCAGTTTC TGCTCAGCAC TCAAATGGAT 
GATTTGATGA AGTCACAGAA AACAGTCCAG GATGCTGTGA AGAAGCTGGA AGGGCCGCCG 
TCTCAACAAG TCATCGAGGA AGCAACACTT TGCCACCTGC GACCTGTTCG TCTGCCACTG 
AACAACTGTG TGTTTTGCAA AGCAGATGAA CTTTTTACAG ACTACGAGTC GAAGCTTTTC 
TCGCACACAG TAAAAGGGCA GACGGCTATC TTTGAGGAGA TGATTGAAGA CGAGGAAGGG 
CTGGTAGATG ACAGGCTTCC CACCACCAGT AGAGGATTAT GGGCCGCCAG TGAGACCGAA 
CGAGCTCTTA AAGCCATCTT GTCCTTCGCT AAAGTCCGCC GCTTGGAGTC TGGCCTCATC 
GAGGAGGGAA ACTCCTTCAT GGAGCTCTTC GAGTCCTGGA AGAAAGAATA CAAGCTTCTG 
CATGAGTACT GGATGTCTCT GCGGGATCAT GTATCTGCTA TAGATGAGCT GGGAATGGCC 
ACCGAGAGGC TGCGTGTGCG CTTACCTGAT GAGCCCAAAC CCAAAGTCCT GCATATTATT 
GAGCCTCACG AGGTGGAGCA GAACAGGATA AAGCTGCTCA ATGACAGGGC AGTGGCCAAA 
TCACAGCTGC AAAAAAAGCT TGGGCAGTTT CTCTATCTCA CAAACCTGGA GAAGTCTCAG 
GATAAGTCCA CTGGTGGTCT GAATCCAGAA CCATGTCCAA TCTGTGCTCG TCCGCTGGGT 
CAGGAGTGGG CCGTACTGAC CTGTGGACAC TGTTTCTGTA ACGAGTGCAT CGCCATCATT 
GTGGAGCAAT ACAGCATCGG CAATCGACGG AGAGCTATAA AGTGTGCCAT ATGCCGACAA 
ACCACTTCAC ACGCAGAGAT CTCATATGTG TTCACCACTC AAAACAACCA CCAGGGGCAG 
GACATCCCTG TGAAGGGTAG CCACTCCACG AAGGTCGAGG CTGTGGTTCG AGTCCTAAAA 
AAAGTCCAGA TGACCGACCC GGGAGCCAAG TCTCTTGTGT TCTCCACGTG GCAGGGCGTC 
CTGGACATCA TCGCCAAAGC CCTGTTTGAC AACAACATGG AGTTTGCACA GATCAACGGA 
ATTCACAAGT TTCAGGAGAA CCTGAGTGCT TTCAAATATG AGGAGAAGAT CAACATCTTG 
TTGCTGCCAC TGCACACAGG CTCCAATGGA CTGAACATCA TTGAAGCAAC ACACGTGCTA 
CTGGTGGAGC CCATCCTAAA CCCCGCCCAC GAGCTGCAGG CCATAGGAAG AGTGCATCGG 
ATTGGCCAGA CCAAACCCAC ATTTGTTCAT CGGTTTCTGA TTAAGTCCAC CATTGAGGAA 
CGGATGCAAG CCATGCTGAA AACAGCAGAA AAGAGTCACA GTTCCACGTC TATGAAGCAT 
TCGGAGGCCT CTGTGTTTAC AGTGGCAGAC CTGGCGGATC TGTTCACCGA GGACAGCGAG 
ACTCTTGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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shprh cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

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FAX

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