LIG3 cDNA ORF clone, Alligator sinensis(Chinese alligator)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIG3
Entrez Gene ID	102376796
Full Name	DNA ligase 3
Gene Type	protein-coding
Organism	Alligator sinensis(Chinese alligator)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006025747.2	XP_006025809.1	LOW QUALITY PROTEIN: DNA ligase 3
XM_006025747.3	XP_006025809.1	LOW QUALITY PROTEIN: DNA ligase 3

Pathways from BioSystems
Gene Ontology

The following LIG3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIG3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAl20129	XM_006025747.3 Latest version!	Alligator sinensis DNA ligase 3 (LIG3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OAl20129	XM_006025747.2	Alligator sinensis ligase III, DNA, ATP-dependent (LIG3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIG3 ( XM_006025747.2 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006025809.1 Alligator sinensis ligase III, DNA, ATP-dependent (LIG3), mRNA.

Price & Availability↑

CloneID	OAl20129
Clone ID Related Accession (Same CDS sequence)	XM_006025747.2 , XM_006025747.3
Accession Version	XM_006025747.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-29
Organism	Alligator sinensis(Chinese alligator)
Product	LOW QUALITY PROTEIN: DNA ligase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005842165.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 30, 2015 this sequence version replaced XM_006025747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Alligator sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGCGGAAC AGCGGTACTG CGTGGACTAC GCCAAGCGCG GCACGGCCGG CTGCAAGAAG 
TGCAAGGAGA AGATCCTGAA GGGCATGGTC CGCATTGGGA AGGTTGTGCC CAACCCCTTC 
ACCGAGTCGG GCGGCGACAT GAAGGAGTGG TACCACGTGA AGTGCATATT CGAGAAGCTG 
GAGCGGGCTC GTGCCACCAC CAAGAAGATC GAGGACATCA CAGACCTCGA GGGGTGGGAG 
GAGCTGCAAG ACGCCGAGAA AGACCTCATC AACCAGCTTA TCTCGGAGGC CACATCCAAG 
TCTGCAAGCA CACCTAAGAA GAAGGCAACA GTCCAGGCTA AGCTGTCCCC CAGTGGGCAG 
ATAACCTCCC CCAAGAAAGA TCCAGGAGCT GTTCCTAATC CATCTCCAAA GAAATTCTCT 
GGTTTCACAG CTAAACCAGG CAGTTCTGAG GACACCCCTG CAAATTCTTC CCACAAGTCT 
ACTCTGTCTT CAAAGAAGTG TGACCCAAAG CACAAGGATT GTTTGTTGCG GGAATTCAGA 
AAATTGTGCG CCATGGTTGC TGACAAACCC AGCTATAACG TGAAAACACA GATCATCCAG 
GACTTTCTGC AGAAAGGGAC TGGGGGAGAT GGCTTTCATG GTGACTTGTA CCTGACTATT 
AAGCTGCTGT TGCCGGGTGT CATTAAAATT GTGTACAACT TGAATGACAA GCAGATTGTA 
AAGCTGTTCA GCAGAATTTT CAACTGCAGC CAGGATGAAA TGGTCCGGGA CCTGGAGCAG 
GGAGATGTTT CAGAAACAGT CCGCATCTTT TTTGAGGAGA GCAGGTCCTG TCCTCCAGCT 
GCCAAAAGTC TCCTCACAAT CCAGGAAGTG GACGAGTTCC TAATCGGACT GTCAAAGCTT 
ACAAAGGAGG ATGACCAGCA AACTCTGCTT CAGGGTATCA CTCGCAGGTG CACGGGCAAT 
GACCTGAAAT GCATCATCAG GCTTATAAAG CACGATTTGA AAATGAATGC TGGTGCAAAG 
CATGTGTTAG ATGCTTTGGA TCCAAATGCC TATGAGGCAT TCAAAGCATC ACGCAATCTT 
CAGGATGTTG TGGAGCGTGT GCTAAGAAAC CAGCAGGAGG CAGAGAAGCA GCCGGGTCTG 
AAGCGAACCC TCAGTGTGCA GGCCTCTCTG ATGACCCCTG TTCAGCCTAT GCTGGCTGAA 
GCCTGCAAGT CAATAGAATA TGCCATGAAG AAGTGTCCGA ATGGGATGTA CGCAGAGATC 
AAGTATGATG GGGAGCGAGT GCAGGTCCAC AAGAATGGAG ATCATTTCAG CTACTTCAGC 
CGAAGCCTCA AGCCAGTCCT TCCTCACAAA GTGGCCCATT TTAAGGACTT CATCCCCAGA 
GCTTTTCCTG GTGGACACAG TATGATCTTG GATTCAGAAG TTCTTCTCAT TGACAACAAA 
ACAGGCAAAC CACTCCCTTT TGGGACCCTT GGAGTCCACA AGAAAGCTGC TTTCCAAGAT 
GCCAATGTCT GCTTGTTTGT ATTTGATTGT ATCTACTTCA ATGATGTCAG CCTGATGGAC 
AGGCCTTTGT GTGAGCGTCG TAAGTTTCTC CATGATAACA TGGTTGAAAT CCCCNAGCCG 
GATCCTCTTC TCCGAGATGA AGCATGTCAC AAAAGCTTCA GATCTGGCAG ATATGATAAT 
AGAGTCATCC GTGAGGGACT GGAAGGACTG GTGCTGAAGG ATATAAAGAG CACGTATGAG 
CCAGGAAAAC GTCACTGGCT GAAAGTGAAA AAGGACTATC TAAATGAAGG TGCCATGGCT 
GATACAGCAG ACCTGGTGGT TCTGGGAGCT TTCTACGGAC AAGGCAGTAA AGGTGGAATG 
ATGTCAATTT TCCTAATGGG CTGCTATGAT CCCAAGACTG AAAAATGGTG TACGGTGACA 
AAGTGCTCTG GTGGGCATGA TGATGCCACC TTAGCTCGCC TGCAGAATGA GCTGGACATG 
GTGAAGATCA GCAAGGATCC CAATAAAATT CCAAGCTGGC TAAAAATAAA TAAGCTCTAC 
TATCCAGATT TCATTGTTCC AGACCCAAAG AAGGCCCCAG TGTGGGAGAT AACAGGTGCG 
GAGTTCTCTA AAGCAGAAGC CCACACGGCA GATGGGATTT CCATTCGTTT CCCTCGTTGT 
ACCCGGATCC GAGATGACAA AGACTGGAAG ACAGCTACCA ATCTCCCTCA ACTCAAGGAG 
CTGTACCAGC TCTCCAAAGA GAAGTCTGAT TTCAGTGTTG TTGCTGGAGA GGAGGAGGTA 
TCTCCAGCTG GTAGCAGTGG AGAGAATGAT GGAAATTCAG GGTCTTCCAC ACCACACAAT 
ACTCCTAAAA CTCCCCCAAG GAAGTCGCCT GCAAAAACCA AGAAGTCTGA AGGTAACAAT 
TTAGTCTATG TGACCCCCGA AAAGAAAGTG GAGGATAAAC GAGGGGAGAA GCGGAAAGCA 
GCCGAGCTGG ATGACAATGG GAAGAAGATA TTGCTGGACA TATTTACAGG GGTGAGGCTG 
TACCTACCAC CCTCCGTGGA GAACTTCAGC AAAATCCGGC GGTACTTTGT GGCATATGAT 
GGGGACCTTG TACAAGAGTT TGATACGGCT TCTGCAACAC ATGTGATAGG GGACCTTGAG 
GAGAACCCTG GAGCTAAGCG TGTGTCACCC AGTTGGATCT GGGAGTGTAT CCGCAAGCGG 
AGACTGGTAG CCCCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006025809.1
CDS	17..2737
Translation	MAEQRYCVDYAKRGTAGCKKCKEKILKGMVRIGKVVPNPFTESGGDMKEWYHVKCIFEKLERARATTKKIEDITDLEGWEELQDAEKDLINQLISEATSKSASTPKKKATVQAKLSPSGQITSPKKDPGAVPNPSPKKFSGFTAKPGSSEDTPANSSHKSTLSSKKCDPKHKDCLLREFRKLCAMVADKPSYNVKTQIIQDFLQKGTGGDGFHGDLYLTIKLLLPGVIKIVYNLNDKQIVKLFSRIFNCSQDEMVRDLEQGDVSETVRIFFEESRSCPPAAKSLLTIQEVDEFLIGLSKLTKEDDQQTLLQGITRRCTGNDLKCIIRLIKHDLKMNAGAKHVLDALDPNAYEAFKASRNLQDVVERVLRNQQEAEKQPGLKRTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDFIPRAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPnullPDPLLRDEACHKSFRSGRYDNRVIREGLEGLVLKDIKSTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPKTEKWCTVTKCSGGHDDATLARLQNELDMVKISKDPNKIPSWLKINKLYYPDFIVPDPKKAPVWEITGAEFSKAEAHTADGISIRFPRCTRIRDDKDWKTATNLPQLKELYQLSKEKSDFSVVAGEEEVSPAGSSGENDGNSGSSTPHNTPKTPPRKSPAKTKKSEGNNLVYVTPEKKVEDKRGEKRKAAELDDNGKKILLDIFTGVRLYLPPSVENFSKIRRYFVAYDGDLVQEFDTASATHVIGDLEENPGAKRVSPSWIWECIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_006025747.2
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis ligase III, DNA, ATP-dependent (LIG3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006025747.2

ORF Insert Sequence:

ATGGCGGAAC AGCGGTACTG CGTGGACTAC GCCAAGCGCG GCACGGCCGG CTGCAAGAAG 
TGCAAGGAGA AGATCCTGAA GGGCATGGTC CGCATTGGGA AGGTTGTGCC CAACCCCTTC 
ACCGAGTCGG GCGGCGACAT GAAGGAGTGG TACCACGTGA AGTGCATATT CGAGAAGCTG 
GAGCGGGCTC GTGCCACCAC CAAGAAGATC GAGGACATCA CAGACCTCGA GGGGTGGGAG 
GAGCTGCAAG ACGCCGAGAA AGACCTCATC AACCAGCTTA TCTCGGAGGC CACATCCAAG 
TCTGCAAGCA CACCTAAGAA GAAGGCAACA GTCCAGGCTA AGCTGTCCCC CAGTGGGCAG 
ATAACCTCCC CCAAGAAAGA TCCAGGAGCT GTTCCTAATC CATCTCCAAA GAAATTCTCT 
GGTTTCACAG CTAAACCAGG CAGTTCTGAG GACACCCCTG CAAATTCTTC CCACAAGTCT 
ACTCTGTCTT CAAAGAAGTG TGACCCAAAG CACAAGGATT GTTTGTTGCG GGAATTCAGA 
AAATTGTGCG CCATGGTTGC TGACAAACCC AGCTATAACG TGAAAACACA GATCATCCAG 
GACTTTCTGC AGAAAGGGAC TGGGGGAGAT GGCTTTCATG GTGACTTGTA CCTGACTATT 
AAGCTGCTGT TGCCGGGTGT CATTAAAATT GTGTACAACT TGAATGACAA GCAGATTGTA 
AAGCTGTTCA GCAGAATTTT CAACTGCAGC CAGGATGAAA TGGTCCGGGA CCTGGAGCAG 
GGAGATGTTT CAGAAACAGT CCGCATCTTT TTTGAGGAGA GCAGGTCCTG TCCTCCAGCT 
GCCAAAAGTC TCCTCACAAT CCAGGAAGTG GACGAGTTCC TAATCGGACT GTCAAAGCTT 
ACAAAGGAGG ATGACCAGCA AACTCTGCTT CAGGGTATCA CTCGCAGGTG CACGGGCAAT 
GACCTGAAAT GCATCATCAG GCTTATAAAG CACGATTTGA AAATGAATGC TGGTGCAAAG 
CATGTGTTAG ATGCTTTGGA TCCAAATGCC TATGAGGCAT TCAAAGCATC ACGCAATCTT 
CAGGATGTTG TGGAGCGTGT GCTAAGAAAC CAGCAGGAGG CAGAGAAGCA GCCGGGTCTG 
AAGCGAACCC TCAGTGTGCA GGCCTCTCTG ATGACCCCTG TTCAGCCTAT GCTGGCTGAA 
GCCTGCAAGT CAATAGAATA TGCCATGAAG AAGTGTCCGA ATGGGATGTA CGCAGAGATC 
AAGTATGATG GGGAGCGAGT GCAGGTCCAC AAGAATGGAG ATCATTTCAG CTACTTCAGC 
CGAAGCCTCA AGCCAGTCCT TCCTCACAAA GTGGCCCATT TTAAGGACTT CATCCCCAGA 
GCTTTTCCTG GTGGACACAG TATGATCTTG GATTCAGAAG TTCTTCTCAT TGACAACAAA 
ACAGGCAAAC CACTCCCTTT TGGGACCCTT GGAGTCCACA AGAAAGCTGC TTTCCAAGAT 
GCCAATGTCT GCTTGTTTGT ATTTGATTGT ATCTACTTCA ATGATGTCAG CCTGATGGAC 
AGGCCTTTGT GTGAGCGTCG TAAGTTTCTC CATGATAACA TGGTTGAAAT CCCCNAGCCG 
GATCCTCTTC TCCGAGATGA AGCATGTCAC AAAAGCTTCA GATCTGGCAG ATATGATAAT 
AGAGTCATCC GTGAGGGACT GGAAGGACTG GTGCTGAAGG ATATAAAGAG CACGTATGAG 
CCAGGAAAAC GTCACTGGCT GAAAGTGAAA AAGGACTATC TAAATGAAGG TGCCATGGCT 
GATACAGCAG ACCTGGTGGT TCTGGGAGCT TTCTACGGAC AAGGCAGTAA AGGTGGAATG 
ATGTCAATTT TCCTAATGGG CTGCTATGAT CCCAAGACTG AAAAATGGTG TACGGTGACA 
AAGTGCTCTG GTGGGCATGA TGATGCCACC TTAGCTCGCC TGCAGAATGA GCTGGACATG 
GTGAAGATCA GCAAGGATCC CAATAAAATT CCAAGCTGGC TAAAAATAAA TAAGCTCTAC 
TATCCAGATT TCATTGTTCC AGACCCAAAG AAGGCCCCAG TGTGGGAGAT AACAGGTGCG 
GAGTTCTCTA AAGCAGAAGC CCACACGGCA GATGGGATTT CCATTCGTTT CCCTCGTTGT 
ACCCGGATCC GAGATGACAA AGACTGGAAG ACAGCTACCA ATCTCCCTCA ACTCAAGGAG 
CTGTACCAGC TCTCCAAAGA GAAGTCTGAT TTCAGTGTTG TTGCTGGAGA GGAGGAGGTA 
TCTCCAGCTG GTAGCAGTGG AGAGAATGAT GGAAATTCAG GGTCTTCCAC ACCACACAAT 
ACTCCTAAAA CTCCCCCAAG GAAGTCGCCT GCAAAAACCA AGAAGTCTGA AGGTAACAAT 
TTAGTCTATG TGACCCCCGA AAAGAAAGTG GAGGATAAAC GAGGGGAGAA GCGGAAAGCA 
GCCGAGCTGG ATGACAATGG GAAGAAGATA TTGCTGGACA TATTTACAGG GGTGAGGCTG 
TACCTACCAC CCTCCGTGGA GAACTTCAGC AAAATCCGGC GGTACTTTGT GGCATATGAT 
GGGGACCTTG TACAAGAGTT TGATACGGCT TCTGCAACAC ATGTGATAGG GGACCTTGAG 
GAGAACCCTG GAGCTAAGCG TGTGTCACCC AGTTGGATCT GGGAGTGTAT CCGCAAGCGG 
AGACTGGTAG CCCCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG3 ( XM_006025747.3 ) cDNA ORF clone, Alligator sinensis(Chinese alligator) -> XP_006025809.1 Alligator sinensis DNA ligase 3 (LIG3), mRNA.

Price & Availability↑

CloneID	OAl20129
Clone ID Related Accession (Same CDS sequence)	XM_006025747.2 , XM_006025747.3
Accession Version	XM_006025747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Alligator sinensis(Chinese alligator)
Product	LOW QUALITY PROTEIN: DNA ligase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005842165.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_006025747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Alligator sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGCGGAAC AGCGGTACTG CGTGGACTAC GCCAAGCGCG GCACGGCCGG CTGCAAGAAG 
TGCAAGGAGA AGATCCTGAA GGGCATGGTC CGCATTGGGA AGGTTGTGCC CAACCCCTTC 
ACCGAGTCGG GCGGCGACAT GAAGGAGTGG TACCACGTGA AGTGCATATT CGAGAAGCTG 
GAGCGGGCTC GTGCCACCAC CAAGAAGATC GAGGACATCA CAGACCTCGA GGGGTGGGAG 
GAGCTGCAAG ACGCCGAGAA AGACCTCATC AACCAGCTTA TCTCGGAGGC CACATCCAAG 
TCTGCAAGCA CACCTAAGAA GAAGGCAACA GTCCAGGCTA AGCTGTCCCC CAGTGGGCAG 
ATAACCTCCC CCAAGAAAGA TCCAGGAGCT GTTCCTAATC CATCTCCAAA GAAATTCTCT 
GGTTTCACAG CTAAACCAGG CAGTTCTGAG GACACCCCTG CAAATTCTTC CCACAAGTCT 
ACTCTGTCTT CAAAGAAGTG TGACCCAAAG CACAAGGATT GTTTGTTGCG GGAATTCAGA 
AAATTGTGCG CCATGGTTGC TGACAAACCC AGCTATAACG TGAAAACACA GATCATCCAG 
GACTTTCTGC AGAAAGGGAC TGGGGGAGAT GGCTTTCATG GTGACTTGTA CCTGACTATT 
AAGCTGCTGT TGCCGGGTGT CATTAAAATT GTGTACAACT TGAATGACAA GCAGATTGTA 
AAGCTGTTCA GCAGAATTTT CAACTGCAGC CAGGATGAAA TGGTCCGGGA CCTGGAGCAG 
GGAGATGTTT CAGAAACAGT CCGCATCTTT TTTGAGGAGA GCAGGTCCTG TCCTCCAGCT 
GCCAAAAGTC TCCTCACAAT CCAGGAAGTG GACGAGTTCC TAATCGGACT GTCAAAGCTT 
ACAAAGGAGG ATGACCAGCA AACTCTGCTT CAGGGTATCA CTCGCAGGTG CACGGGCAAT 
GACCTGAAAT GCATCATCAG GCTTATAAAG CACGATTTGA AAATGAATGC TGGTGCAAAG 
CATGTGTTAG ATGCTTTGGA TCCAAATGCC TATGAGGCAT TCAAAGCATC ACGCAATCTT 
CAGGATGTTG TGGAGCGTGT GCTAAGAAAC CAGCAGGAGG CAGAGAAGCA GCCGGGTCTG 
AAGCGAACCC TCAGTGTGCA GGCCTCTCTG ATGACCCCTG TTCAGCCTAT GCTGGCTGAA 
GCCTGCAAGT CAATAGAATA TGCCATGAAG AAGTGTCCGA ATGGGATGTA CGCAGAGATC 
AAGTATGATG GGGAGCGAGT GCAGGTCCAC AAGAATGGAG ATCATTTCAG CTACTTCAGC 
CGAAGCCTCA AGCCAGTCCT TCCTCACAAA GTGGCCCATT TTAAGGACTT CATCCCCAGA 
GCTTTTCCTG GTGGACACAG TATGATCTTG GATTCAGAAG TTCTTCTCAT TGACAACAAA 
ACAGGCAAAC CACTCCCTTT TGGGACCCTT GGAGTCCACA AGAAAGCTGC TTTCCAAGAT 
GCCAATGTCT GCTTGTTTGT ATTTGATTGT ATCTACTTCA ATGATGTCAG CCTGATGGAC 
AGGCCTTTGT GTGAGCGTCG TAAGTTTCTC CATGATAACA TGGTTGAAAT CCCCNAGCCG 
GATCCTCTTC TCCGAGATGA AGCATGTCAC AAAAGCTTCA GATCTGGCAG ATATGATAAT 
AGAGTCATCC GTGAGGGACT GGAAGGACTG GTGCTGAAGG ATATAAAGAG CACGTATGAG 
CCAGGAAAAC GTCACTGGCT GAAAGTGAAA AAGGACTATC TAAATGAAGG TGCCATGGCT 
GATACAGCAG ACCTGGTGGT TCTGGGAGCT TTCTACGGAC AAGGCAGTAA AGGTGGAATG 
ATGTCAATTT TCCTAATGGG CTGCTATGAT CCCAAGACTG AAAAATGGTG TACGGTGACA 
AAGTGCTCTG GTGGGCATGA TGATGCCACC TTAGCTCGCC TGCAGAATGA GCTGGACATG 
GTGAAGATCA GCAAGGATCC CAATAAAATT CCAAGCTGGC TAAAAATAAA TAAGCTCTAC 
TATCCAGATT TCATTGTTCC AGACCCAAAG AAGGCCCCAG TGTGGGAGAT AACAGGTGCG 
GAGTTCTCTA AAGCAGAAGC CCACACGGCA GATGGGATTT CCATTCGTTT CCCTCGTTGT 
ACCCGGATCC GAGATGACAA AGACTGGAAG ACAGCTACCA ATCTCCCTCA ACTCAAGGAG 
CTGTACCAGC TCTCCAAAGA GAAGTCTGAT TTCAGTGTTG TTGCTGGAGA GGAGGAGGTA 
TCTCCAGCTG GTAGCAGTGG AGAGAATGAT GGAAATTCAG GGTCTTCCAC ACCACACAAT 
ACTCCTAAAA CTCCCCCAAG GAAGTCGCCT GCAAAAACCA AGAAGTCTGA AGGTAACAAT 
TTAGTCTATG TGACCCCCGA AAAGAAAGTG GAGGATAAAC GAGGGGAGAA GCGGAAAGCA 
GCCGAGCTGG ATGACAATGG GAAGAAGATA TTGCTGGACA TATTTACAGG GGTGAGGCTG 
TACCTACCAC CCTCCGTGGA GAACTTCAGC AAAATCCGGC GGTACTTTGT GGCATATGAT 
GGGGACCTTG TACAAGAGTT TGATACGGCT TCTGCAACAC ATGTGATAGG GGACCTTGAG 
GAGAACCCTG GAGCTAAGCG TGTGTCACCC AGTTGGATCT GGGAGTGTAT CCGCAAGCGG 
AGACTGGTAG CCCCCTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006025809.1
CDS	32..2752
Translation	MAEQRYCVDYAKRGTAGCKKCKEKILKGMVRIGKVVPNPFTESGGDMKEWYHVKCIFEKLERARATTKKIEDITDLEGWEELQDAEKDLINQLISEATSKSASTPKKKATVQAKLSPSGQITSPKKDPGAVPNPSPKKFSGFTAKPGSSEDTPANSSHKSTLSSKKCDPKHKDCLLREFRKLCAMVADKPSYNVKTQIIQDFLQKGTGGDGFHGDLYLTIKLLLPGVIKIVYNLNDKQIVKLFSRIFNCSQDEMVRDLEQGDVSETVRIFFEESRSCPPAAKSLLTIQEVDEFLIGLSKLTKEDDQQTLLQGITRRCTGNDLKCIIRLIKHDLKMNAGAKHVLDALDPNAYEAFKASRNLQDVVERVLRNQQEAEKQPGLKRTLSVQASLMTPVQPMLAEACKSIEYAMKKCPNGMYAEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDFIPRAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPnullPDPLLRDEACHKSFRSGRYDNRVIREGLEGLVLKDIKSTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPKTEKWCTVTKCSGGHDDATLARLQNELDMVKISKDPNKIPSWLKINKLYYPDFIVPDPKKAPVWEITGAEFSKAEAHTADGISIRFPRCTRIRDDKDWKTATNLPQLKELYQLSKEKSDFSVVAGEEEVSPAGSSGENDGNSGSSTPHNTPKTPPRKSPAKTKKSEGNNLVYVTPEKKVEDKRGEKRKAAELDDNGKKILLDIFTGVRLYLPPSVENFSKIRRYFVAYDGDLVQEFDTASATHVIGDLEENPGAKRVSPSWIWECIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_006025747.3
Organism	Alligator sinensis(Chinese alligator)
Definition	Alligator sinensis DNA ligase 3 (LIG3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006025747.3

ORF Insert Sequence:

ATGGCGGAAC AGCGGTACTG CGTGGACTAC GCCAAGCGCG GCACGGCCGG CTGCAAGAAG 
TGCAAGGAGA AGATCCTGAA GGGCATGGTC CGCATTGGGA AGGTTGTGCC CAACCCCTTC 
ACCGAGTCGG GCGGCGACAT GAAGGAGTGG TACCACGTGA AGTGCATATT CGAGAAGCTG 
GAGCGGGCTC GTGCCACCAC CAAGAAGATC GAGGACATCA CAGACCTCGA GGGGTGGGAG 
GAGCTGCAAG ACGCCGAGAA AGACCTCATC AACCAGCTTA TCTCGGAGGC CACATCCAAG 
TCTGCAAGCA CACCTAAGAA GAAGGCAACA GTCCAGGCTA AGCTGTCCCC CAGTGGGCAG 
ATAACCTCCC CCAAGAAAGA TCCAGGAGCT GTTCCTAATC CATCTCCAAA GAAATTCTCT 
GGTTTCACAG CTAAACCAGG CAGTTCTGAG GACACCCCTG CAAATTCTTC CCACAAGTCT 
ACTCTGTCTT CAAAGAAGTG TGACCCAAAG CACAAGGATT GTTTGTTGCG GGAATTCAGA 
AAATTGTGCG CCATGGTTGC TGACAAACCC AGCTATAACG TGAAAACACA GATCATCCAG 
GACTTTCTGC AGAAAGGGAC TGGGGGAGAT GGCTTTCATG GTGACTTGTA CCTGACTATT 
AAGCTGCTGT TGCCGGGTGT CATTAAAATT GTGTACAACT TGAATGACAA GCAGATTGTA 
AAGCTGTTCA GCAGAATTTT CAACTGCAGC CAGGATGAAA TGGTCCGGGA CCTGGAGCAG 
GGAGATGTTT CAGAAACAGT CCGCATCTTT TTTGAGGAGA GCAGGTCCTG TCCTCCAGCT 
GCCAAAAGTC TCCTCACAAT CCAGGAAGTG GACGAGTTCC TAATCGGACT GTCAAAGCTT 
ACAAAGGAGG ATGACCAGCA AACTCTGCTT CAGGGTATCA CTCGCAGGTG CACGGGCAAT 
GACCTGAAAT GCATCATCAG GCTTATAAAG CACGATTTGA AAATGAATGC TGGTGCAAAG 
CATGTGTTAG ATGCTTTGGA TCCAAATGCC TATGAGGCAT TCAAAGCATC ACGCAATCTT 
CAGGATGTTG TGGAGCGTGT GCTAAGAAAC CAGCAGGAGG CAGAGAAGCA GCCGGGTCTG 
AAGCGAACCC TCAGTGTGCA GGCCTCTCTG ATGACCCCTG TTCAGCCTAT GCTGGCTGAA 
GCCTGCAAGT CAATAGAATA TGCCATGAAG AAGTGTCCGA ATGGGATGTA CGCAGAGATC 
AAGTATGATG GGGAGCGAGT GCAGGTCCAC AAGAATGGAG ATCATTTCAG CTACTTCAGC 
CGAAGCCTCA AGCCAGTCCT TCCTCACAAA GTGGCCCATT TTAAGGACTT CATCCCCAGA 
GCTTTTCCTG GTGGACACAG TATGATCTTG GATTCAGAAG TTCTTCTCAT TGACAACAAA 
ACAGGCAAAC CACTCCCTTT TGGGACCCTT GGAGTCCACA AGAAAGCTGC TTTCCAAGAT 
GCCAATGTCT GCTTGTTTGT ATTTGATTGT ATCTACTTCA ATGATGTCAG CCTGATGGAC 
AGGCCTTTGT GTGAGCGTCG TAAGTTTCTC CATGATAACA TGGTTGAAAT CCCCNAGCCG 
GATCCTCTTC TCCGAGATGA AGCATGTCAC AAAAGCTTCA GATCTGGCAG ATATGATAAT 
AGAGTCATCC GTGAGGGACT GGAAGGACTG GTGCTGAAGG ATATAAAGAG CACGTATGAG 
CCAGGAAAAC GTCACTGGCT GAAAGTGAAA AAGGACTATC TAAATGAAGG TGCCATGGCT 
GATACAGCAG ACCTGGTGGT TCTGGGAGCT TTCTACGGAC AAGGCAGTAA AGGTGGAATG 
ATGTCAATTT TCCTAATGGG CTGCTATGAT CCCAAGACTG AAAAATGGTG TACGGTGACA 
AAGTGCTCTG GTGGGCATGA TGATGCCACC TTAGCTCGCC TGCAGAATGA GCTGGACATG 
GTGAAGATCA GCAAGGATCC CAATAAAATT CCAAGCTGGC TAAAAATAAA TAAGCTCTAC 
TATCCAGATT TCATTGTTCC AGACCCAAAG AAGGCCCCAG TGTGGGAGAT AACAGGTGCG 
GAGTTCTCTA AAGCAGAAGC CCACACGGCA GATGGGATTT CCATTCGTTT CCCTCGTTGT 
ACCCGGATCC GAGATGACAA AGACTGGAAG ACAGCTACCA ATCTCCCTCA ACTCAAGGAG 
CTGTACCAGC TCTCCAAAGA GAAGTCTGAT TTCAGTGTTG TTGCTGGAGA GGAGGAGGTA 
TCTCCAGCTG GTAGCAGTGG AGAGAATGAT GGAAATTCAG GGTCTTCCAC ACCACACAAT 
ACTCCTAAAA CTCCCCCAAG GAAGTCGCCT GCAAAAACCA AGAAGTCTGA AGGTAACAAT 
TTAGTCTATG TGACCCCCGA AAAGAAAGTG GAGGATAAAC GAGGGGAGAA GCGGAAAGCA 
GCCGAGCTGG ATGACAATGG GAAGAAGATA TTGCTGGACA TATTTACAGG GGTGAGGCTG 
TACCTACCAC CCTCCGTGGA GAACTTCAGC AAAATCCGGC GGTACTTTGT GGCATATGAT 
GGGGACCTTG TACAAGAGTT TGATACGGCT TCTGCAACAC ATGTGATAGG GGACCTTGAG 
GAGAACCCTG GAGCTAAGCG TGTGTCACCC AGTTGGATCT GGGAGTGTAT CCGCAAGCGG 
AGACTGGTAG CCCCCTGCTA A