tbc1d8 cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbc1d8
Entrez Gene ID	110956147
Full Name	TBC1 domain family member 8
Gene Type	protein-coding
Organism	Acanthochromis polyacanthus(spiny chromis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022201464.1	XP_022057156.1	TBC1 domain family member 8 isoform X1
XM_022201465.1	XP_022057157.1	TBC1 domain family member 8 isoform X2

Pathways from BioSystems
Gene Ontology

The following tbc1d8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc197957	XM_022201465.1 Latest version!	Acanthochromis polyacanthus TBC1 domain family member 8 (tbc1d8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAc197956	XM_022201464.1 Latest version!	Acanthochromis polyacanthus TBC1 domain family member 8 (tbc1d8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbc1d8 ( XM_022201465.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022057157.1 Acanthochromis polyacanthus TBC1 domain family member 8 (tbc1d8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc197957
Clone ID Related Accession (Same CDS sequence)	XM_022201465.1
Accession Version	XM_022201465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	TBC1 domain family member 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTTGG AGCGGGTCTC CACCGGCCTC ATGACCGAGG CGATCCGGGT GAACACGCGG 
CAGCGACAAA GAGAGTTCTC CATGTTCTTG AACATGGACG AGGCGTTCGG GGTCGTCAGT 
CAGCTGGCTG ACATTGCTCT GAGGCGGCTG CTGGACAGCG AAGGCCTGGA GCTGGACCGA 
GTCCTGCAGC AGCCCACAAG GATCACCAAG AGGATTCTGG AGGAGCAGGC CATAAGGGAA 
TACGTTCTCG CTCTGTTTCG ACTTCCTCGT GCCGAGAAAC TCTACGAAGT GGCGTCCTGC 
TCGGTGTGGA CGCCTCACGC TCGATGCCAT ACGGCTGGAA GTCTGTACAC CACCGACAGC 
TACCTGTGTT TCTCCAGCAG AGAGGAAGGA AACTGTATCC TGCTCATACC GCTGTCAGAG 
GTGTTGTCCA TAGAAAAAGC AGAGAGCACC AGCCTCCTTC CCAACCCGGT CATTGTGAGC 
ATTCGGACTA AAAAGGCCTT CCAGCTGATT GAGCTGCAGG ACAGAGATGA GCTGGTGGAA 
AGTCTGAACT CCAGACTTCG ATCTATGCAG TGGAAACAGT CTATGTTCCG CAGCAGGAAG 
GACAGCAAGA GGAGCGTGAG CTCTCCAACA CCCTACTACA CTTTCTACTA TGACACTGGA 
TTCTCTGATG AAGAAGAGAT AGAGGAGCAG GTTCTACGTA ACACCGTCAA CAGCGAAGCT 
CTGATGACAG CTTTTCACCA AAACCAACCA GGAACCAACG GCAAGAAGAG TGCTGAGGAG 
GTGAAGGAGC GGTTATGGGA GGATCACTTC ACCGAGTTCG GTCGTGGCGT CCACATGTTT 
CGCACCGAGA AAATCCAAAG GCTGGTTGCT ATGGGCATAC CAGAGTCGCT GCGAGGGGAG 
CTGTGGATGG CACTTTCTGA TGCATCCTCA GAGCTGGAGT CTCATCAGGG CTACTACACC 
AGCCTGGTGC AGAAATCGAT GGGCCACAGC AGTCTGGCCA CAGAGGAGAT AGAGAGGGAC 
CTTCACCGCT CGCTGCCGGA CCATCCGGCC TTCCAGAACC CGACCGGCAT TGCTGCACTC 
AGGCGTGTCC TGACCGCCTA CGCTCACCGC AACCCAAAGA TTGGATACTG TCAGTCTATG 
AACATCCTGG CATCGGTGTT GCTGCTTTAC GCCAAAGAAG AAGAAGCGTT TTGGCTGCTG 
GTGGCTGTTT GTGAGAGGAT GCTGCCTGAC TACTTCAACC GCAGGGTCAT AGGAGCTCAG 
GTGGATCAGT CGGTGTTTGA GGAGCTGATC AGGGAACGTT TACCAGAGTT GGCCGAACAG 
GTTCCAGACC TCTCGACTCT CTCCTCCGTC TCCCTCTCCT GGTTCCTCAC CCTCTTCCTC 
AGTGTCCTGC CCTTCCAGAG CGCCGTCTGC GTGGTCGACT GCTTCTTCTT CCACGGAATC 
AAAGCCATCT TCCAGCTGGG TTTGGCTGTT CTGGATGCCA ACGCTGCTCA GCTCTCAGCA 
AGCACAGACG ATGGACAGGC ACTGATGATT CTCACAAGTT TCCTGGATCA GGTTGGAGGT 
GAGGATTCGT CTTCTGTCCC GTCCTGTCCG CCTGCTGCAG ATGAAAGCAG CTGCAGCGAT 
GCTGATGTTC CAGCTGTGAG ACACACAAAC ATCACTGACC TCATCAACGA GTCCTATGAG 
AAATTTGGAG ATCTGACAGT GCGGCAGATC GAGAGACTTC GCTGTCGACA CAGAATCCGA 
GTACTGCAGG CTCATGAAGA CACCACCAAA GAAAATGCTT TAAGGCTGGT GACTCCAGAT 
GTTTCCATCT CTCCAGAGAA CCTGGCTGAT GTTTATGATC TCTTCAAGAC GGAGCACTTC 
ATCAGTTTAT ATTGGGGCGA CAGCAGCTCT GCAGCAGCAG CCGAGGCAGC AGCTTGGCGT 
TACGGAGACT CTGGTCGCTC CTACGTGGAG CGTCAGTATC GGCTGGACCG ACCCCAGTTT 
AAAAGCCTGT ACGGACTGCT GGTCCCGTGG CCTGGAGGCT CAAACCAGCA CACTGACACT 
CTGGCCAACC GAACCTTCAC CTTGCTGGAC CAAGACCGAG ACAACCTGGT CACCTTTAGA 
GAGTTTGCCA GCTGGCTGGA CACTTTGTAC TGTGAAGAGC TGAATGAGAA AATTCGGCTG 
CTGTATCGGC TTCACATCCC TCCAGCTTTG ACTGAATGTG AGGCCGACCC CTCCCTGATG 
AAAAGCCCCC TGCTGTCCAC AAACAGACCA CTTTATGTTA ATCTGTCCTC TGGTGATGAA 
GATGAAGTTA AAGACTACCA GGAGCAGCTG AAGCAGATGC TGCAGGATCT GATGAAAGAA 
AAGGAGAAAG ACGTAGAGAA GCCTTTGCCA CTCATGAACC AGCGGGAGTT CATCCAGTTC 
TGTAAAACGC TCTACAGCAT GTTCCACAGT GACCCTGAGG AGAATGACCT CTTTCAGGCT 
ATCGCCACGG TAACCAGTCT GGTGCTGCAG ATCGGCGAGG CTGGCCACAA AGGACAAACA 
TCGGGGTCGG AGGTCATCAG CCAAGAGAAG GCGAAAGCGG CGGCACCCCC TCAGGCTGAG 
GACACCAGCG GTGACGGGTG CTCGGAGAGC GAGTGGACGG TCAGCTATGC TCAGATCCTG 
GCCTCACTGC TCACTGAACA GGCGCTGGTG AACTTTTTCG AGAAGCCGGT GGATCTGACG 
GCGAAAATCG CAGAAGCCAA GGAGAGCCAG TATCACCAGC GGGCGGGTTT GCTCACTCTG 
CAACAAGGGA CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022057157.1
CDS	114..2891
Translation	MRLERVSTGLMTEAIRVNTRQRQREFSMFLNMDEAFGVVSQLADIALRRLLDSEGLELDRVLQQPTRITKRILEEQAIREYVLALFRLPRAEKLYEVASCSVWTPHARCHTAGSLYTTDSYLCFSSREEGNCILLIPLSEVLSIEKAESTSLLPNPVIVSIRTKKAFQLIELQDRDELVESLNSRLRSMQWKQSMFRSRKDSKRSVSSPTPYYTFYYDTGFSDEEEIEEQVLRNTVNSEALMTAFHQNQPGTNGKKSAEEVKERLWEDHFTEFGRGVHMFRTEKIQRLVAMGIPESLRGELWMALSDASSELESHQGYYTSLVQKSMGHSSLATEEIERDLHRSLPDHPAFQNPTGIAALRRVLTAYAHRNPKIGYCQSMNILASVLLLYAKEEEAFWLLVAVCERMLPDYFNRRVIGAQVDQSVFEELIRERLPELAEQVPDLSTLSSVSLSWFLTLFLSVLPFQSAVCVVDCFFFHGIKAIFQLGLAVLDANAAQLSASTDDGQALMILTSFLDQVGGEDSSSVPSCPPAADESSCSDADVPAVRHTNITDLINESYEKFGDLTVRQIERLRCRHRIRVLQAHEDTTKENALRLVTPDVSISPENLADVYDLFKTEHFISLYWGDSSSAAAAEAAAWRYGDSGRSYVERQYRLDRPQFKSLYGLLVPWPGGSNQHTDTLANRTFTLLDQDRDNLVTFREFASWLDTLYCEELNEKIRLLYRLHIPPALTECEADPSLMKSPLLSTNRPLYVNLSSGDEDEVKDYQEQLKQMLQDLMKEKEKDVEKPLPLMNQREFIQFCKTLYSMFHSDPEENDLFQAIATVTSLVLQIGEAGHKGQTSGSEVISQEKAKAAAPPQAEDTSGDGCSESEWTVSYAQILASLLTEQALVNFFEKPVDLTAKIAEAKESQYHQRAGLLTLQQGTS

Target ORF information:

RefSeq Version	XM_022201465.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus TBC1 domain family member 8 (tbc1d8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022201465.1

ORF Insert Sequence:

ATGCGGTTGG AGCGGGTCTC CACCGGCCTC ATGACCGAGG CGATCCGGGT GAACACGCGG 
CAGCGACAAA GAGAGTTCTC CATGTTCTTG AACATGGACG AGGCGTTCGG GGTCGTCAGT 
CAGCTGGCTG ACATTGCTCT GAGGCGGCTG CTGGACAGCG AAGGCCTGGA GCTGGACCGA 
GTCCTGCAGC AGCCCACAAG GATCACCAAG AGGATTCTGG AGGAGCAGGC CATAAGGGAA 
TACGTTCTCG CTCTGTTTCG ACTTCCTCGT GCCGAGAAAC TCTACGAAGT GGCGTCCTGC 
TCGGTGTGGA CGCCTCACGC TCGATGCCAT ACGGCTGGAA GTCTGTACAC CACCGACAGC 
TACCTGTGTT TCTCCAGCAG AGAGGAAGGA AACTGTATCC TGCTCATACC GCTGTCAGAG 
GTGTTGTCCA TAGAAAAAGC AGAGAGCACC AGCCTCCTTC CCAACCCGGT CATTGTGAGC 
ATTCGGACTA AAAAGGCCTT CCAGCTGATT GAGCTGCAGG ACAGAGATGA GCTGGTGGAA 
AGTCTGAACT CCAGACTTCG ATCTATGCAG TGGAAACAGT CTATGTTCCG CAGCAGGAAG 
GACAGCAAGA GGAGCGTGAG CTCTCCAACA CCCTACTACA CTTTCTACTA TGACACTGGA 
TTCTCTGATG AAGAAGAGAT AGAGGAGCAG GTTCTACGTA ACACCGTCAA CAGCGAAGCT 
CTGATGACAG CTTTTCACCA AAACCAACCA GGAACCAACG GCAAGAAGAG TGCTGAGGAG 
GTGAAGGAGC GGTTATGGGA GGATCACTTC ACCGAGTTCG GTCGTGGCGT CCACATGTTT 
CGCACCGAGA AAATCCAAAG GCTGGTTGCT ATGGGCATAC CAGAGTCGCT GCGAGGGGAG 
CTGTGGATGG CACTTTCTGA TGCATCCTCA GAGCTGGAGT CTCATCAGGG CTACTACACC 
AGCCTGGTGC AGAAATCGAT GGGCCACAGC AGTCTGGCCA CAGAGGAGAT AGAGAGGGAC 
CTTCACCGCT CGCTGCCGGA CCATCCGGCC TTCCAGAACC CGACCGGCAT TGCTGCACTC 
AGGCGTGTCC TGACCGCCTA CGCTCACCGC AACCCAAAGA TTGGATACTG TCAGTCTATG 
AACATCCTGG CATCGGTGTT GCTGCTTTAC GCCAAAGAAG AAGAAGCGTT TTGGCTGCTG 
GTGGCTGTTT GTGAGAGGAT GCTGCCTGAC TACTTCAACC GCAGGGTCAT AGGAGCTCAG 
GTGGATCAGT CGGTGTTTGA GGAGCTGATC AGGGAACGTT TACCAGAGTT GGCCGAACAG 
GTTCCAGACC TCTCGACTCT CTCCTCCGTC TCCCTCTCCT GGTTCCTCAC CCTCTTCCTC 
AGTGTCCTGC CCTTCCAGAG CGCCGTCTGC GTGGTCGACT GCTTCTTCTT CCACGGAATC 
AAAGCCATCT TCCAGCTGGG TTTGGCTGTT CTGGATGCCA ACGCTGCTCA GCTCTCAGCA 
AGCACAGACG ATGGACAGGC ACTGATGATT CTCACAAGTT TCCTGGATCA GGTTGGAGGT 
GAGGATTCGT CTTCTGTCCC GTCCTGTCCG CCTGCTGCAG ATGAAAGCAG CTGCAGCGAT 
GCTGATGTTC CAGCTGTGAG ACACACAAAC ATCACTGACC TCATCAACGA GTCCTATGAG 
AAATTTGGAG ATCTGACAGT GCGGCAGATC GAGAGACTTC GCTGTCGACA CAGAATCCGA 
GTACTGCAGG CTCATGAAGA CACCACCAAA GAAAATGCTT TAAGGCTGGT GACTCCAGAT 
GTTTCCATCT CTCCAGAGAA CCTGGCTGAT GTTTATGATC TCTTCAAGAC GGAGCACTTC 
ATCAGTTTAT ATTGGGGCGA CAGCAGCTCT GCAGCAGCAG CCGAGGCAGC AGCTTGGCGT 
TACGGAGACT CTGGTCGCTC CTACGTGGAG CGTCAGTATC GGCTGGACCG ACCCCAGTTT 
AAAAGCCTGT ACGGACTGCT GGTCCCGTGG CCTGGAGGCT CAAACCAGCA CACTGACACT 
CTGGCCAACC GAACCTTCAC CTTGCTGGAC CAAGACCGAG ACAACCTGGT CACCTTTAGA 
GAGTTTGCCA GCTGGCTGGA CACTTTGTAC TGTGAAGAGC TGAATGAGAA AATTCGGCTG 
CTGTATCGGC TTCACATCCC TCCAGCTTTG ACTGAATGTG AGGCCGACCC CTCCCTGATG 
AAAAGCCCCC TGCTGTCCAC AAACAGACCA CTTTATGTTA ATCTGTCCTC TGGTGATGAA 
GATGAAGTTA AAGACTACCA GGAGCAGCTG AAGCAGATGC TGCAGGATCT GATGAAAGAA 
AAGGAGAAAG ACGTAGAGAA GCCTTTGCCA CTCATGAACC AGCGGGAGTT CATCCAGTTC 
TGTAAAACGC TCTACAGCAT GTTCCACAGT GACCCTGAGG AGAATGACCT CTTTCAGGCT 
ATCGCCACGG TAACCAGTCT GGTGCTGCAG ATCGGCGAGG CTGGCCACAA AGGACAAACA 
TCGGGGTCGG AGGTCATCAG CCAAGAGAAG GCGAAAGCGG CGGCACCCCC TCAGGCTGAG 
GACACCAGCG GTGACGGGTG CTCGGAGAGC GAGTGGACGG TCAGCTATGC TCAGATCCTG 
GCCTCACTGC TCACTGAACA GGCGCTGGTG AACTTTTTCG AGAAGCCGGT GGATCTGACG 
GCGAAAATCG CAGAAGCCAA GGAGAGCCAG TATCACCAGC GGGCGGGTTT GCTCACTCTG 
CAACAAGGGA CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d8 ( XM_022201464.1 ) cDNA ORF clone, Acanthochromis polyacanthus(spiny chromis) -> XP_022057156.1 Acanthochromis polyacanthus TBC1 domain family member 8 (tbc1d8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc197956
Clone ID Related Accession (Same CDS sequence)	XM_022201464.1
Accession Version	XM_022201464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-06
Organism	Acanthochromis polyacanthus(spiny chromis)
Product	TBC1 domain family member 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019029565.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthochromis polyacanthus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTGA ACCCGGAGGA AGTTCTGCTG AAAAACGCTC TGAAGCTCTG GGTAACCGAG 
AGGAGCAACG ACTTCTTCCT GCTGCAGCGG AGGAGAGGCC ACGGAGAGCC CACCAGCAGG 
ATCACAGGGC TCCTGGTGGG GGCTCTGGAC TCAGTGCTGG ACTCCAATGC CAGGGTCACC 
CCGTTCCGAA TCCTGCTCCA GGTTCCTGGT TCCCAGATCA GCTGGGTCAT CGCTAGCGGA 
GCCGTCATCG AGGAGGTGAA CAAGCACTGG GACTGGCTGG TCCACAACCT GCTTCACTCG 
CTGTCGGTGT TTGAGAACAA AGAGGATGTT GCCAGCTTTG TCAAAGGAAA AGTGAAGGGC 
CTCATCGCAG AGGAGGTTCG GGGTCGCCAG GCCGCCCAGG AGGACGATCC GGAGAAGTTC 
AGGGAGGCGC TGCTGAAGTT TGAGCTGCAC TTCGGCCTCC CGTCATCGGA GAAGCTGGTG 
ACATACTTCT CCTGCTGCTG CTGGAAGGGC CGGGTGCCTC GACAGGGCTT CCTCTACCTC 
AGCATCAACC ATATGGCCTT CTACTCCTTC CTGCTGGGGA AGGAAGTTAA GTTTGTGATT 
CCATGGGCAG AGGTGATGCG GTTGGAGCGG GTCTCCACCG GCCTCATGAC CGAGGCGATC 
CGGGTGAACA CGCGGCAGCG ACAAAGAGAG TTCTCCATGT TCTTGAACAT GGACGAGGCG 
TTCGGGGTCG TCAGTCAGCT GGCTGACATT GCTCTGAGGC GGCTGCTGGA CAGCGAAGGC 
CTGGAGCTGG ACCGAGTCCT GCAGCAGCCC ACAAGGATCA CCAAGAGGAT TCTGGAGGAG 
CAGGCCATAA GGGAATACGT TCTCGCTCTG TTTCGACTTC CTCGTGCCGA GAAACTCTAC 
GAAGTGGCGT CCTGCTCGGT GTGGACGCCT CACGCTCGAT GCCATACGGC TGGAAGTCTG 
TACACCACCG ACAGCTACCT GTGTTTCTCC AGCAGAGAGG AAGGAAACTG TATCCTGCTC 
ATACCGCTGT CAGAGGTGTT GTCCATAGAA AAAGCAGAGA GCACCAGCCT CCTTCCCAAC 
CCGGTCATTG TGAGCATTCG GACTAAAAAG GCCTTCCAGC TGATTGAGCT GCAGGACAGA 
GATGAGCTGG TGGAAAGTCT GAACTCCAGA CTTCGATCTA TGCAGTGGAA ACAGTCTATG 
TTCCGCAGCA GGAAGGACAG CAAGAGGAGC GTGAGCTCTC CAACACCCTA CTACACTTTC 
TACTATGACA CTGGATTCTC TGATGAAGAA GAGATAGAGG AGCAGGTTCT ACGTAACACC 
GTCAACAGCG AAGCTCTGAT GACAGCTTTT CACCAAAACC AACCAGGAAC CAACGGCAAG 
AAGAGTGCTG AGGAGGTGAA GGAGCGGTTA TGGGAGGATC ACTTCACCGA GTTCGGTCGT 
GGCGTCCACA TGTTTCGCAC CGAGAAAATC CAAAGGCTGG TTGCTATGGG CATACCAGAG 
TCGCTGCGAG GGGAGCTGTG GATGGCACTT TCTGATGCAT CCTCAGAGCT GGAGTCTCAT 
CAGGGCTACT ACACCAGCCT GGTGCAGAAA TCGATGGGCC ACAGCAGTCT GGCCACAGAG 
GAGATAGAGA GGGACCTTCA CCGCTCGCTG CCGGACCATC CGGCCTTCCA GAACCCGACC 
GGCATTGCTG CACTCAGGCG TGTCCTGACC GCCTACGCTC ACCGCAACCC AAAGATTGGA 
TACTGTCAGT CTATGAACAT CCTGGCATCG GTGTTGCTGC TTTACGCCAA AGAAGAAGAA 
GCGTTTTGGC TGCTGGTGGC TGTTTGTGAG AGGATGCTGC CTGACTACTT CAACCGCAGG 
GTCATAGGAG CTCAGGTGGA TCAGTCGGTG TTTGAGGAGC TGATCAGGGA ACGTTTACCA 
GAGTTGGCCG AACAGGTTCC AGACCTCTCG ACTCTCTCCT CCGTCTCCCT CTCCTGGTTC 
CTCACCCTCT TCCTCAGTGT CCTGCCCTTC CAGAGCGCCG TCTGCGTGGT CGACTGCTTC 
TTCTTCCACG GAATCAAAGC CATCTTCCAG CTGGGTTTGG CTGTTCTGGA TGCCAACGCT 
GCTCAGCTCT CAGCAAGCAC AGACGATGGA CAGGCACTGA TGATTCTCAC AAGTTTCCTG 
GATCAGGTTG GAGGTGAGGA TTCGTCTTCT GTCCCGTCCT GTCCGCCTGC TGCAGATGAA 
AGCAGCTGCA GCGATGCTGA TGTTCCAGCT GTGAGACACA CAAACATCAC TGACCTCATC 
AACGAGTCCT ATGAGAAATT TGGAGATCTG ACAGTGCGGC AGATCGAGAG ACTTCGCTGT 
CGACACAGAA TCCGAGTACT GCAGGCTCAT GAAGACACCA CCAAAGAAAA TGCTTTAAGG 
CTGGTGACTC CAGATGTTTC CATCTCTCCA GAGAACCTGG CTGATGTTTA TGATCTCTTC 
AAGACGGAGC ACTTCATCAG TTTATATTGG GGCGACAGCA GCTCTGCAGC AGCAGCCGAG 
GCAGCAGCTT GGCGTTACGG AGACTCTGGT CGCTCCTACG TGGAGCGTCA GTATCGGCTG 
GACCGACCCC AGTTTAAAAG CCTGTACGGA CTGCTGGTCC CGTGGCCTGG AGGCTCAAAC 
CAGCACACTG ACACTCTGGC CAACCGAACC TTCACCTTGC TGGACCAAGA CCGAGACAAC 
CTGGTCACCT TTAGAGAGTT TGCCAGCTGG CTGGACACTT TGTACTGTGA AGAGCTGAAT 
GAGAAAATTC GGCTGCTGTA TCGGCTTCAC ATCCCTCCAG CTTTGACTGA ATGTGAGGCC 
GACCCCTCCC TGATGAAAAG CCCCCTGCTG TCCACAAACA GACCACTTTA TGTTAATCTG 
TCCTCTGGTG ATGAAGATGA AGTTAAAGAC TACCAGGAGC AGCTGAAGCA GATGCTGCAG 
GATCTGATGA AAGAAAAGGA GAAAGACGTA GAGAAGCCTT TGCCACTCAT GAACCAGCGG 
GAGTTCATCC AGTTCTGTAA AACGCTCTAC AGCATGTTCC ACAGTGACCC TGAGGAGAAT 
GACCTCTTTC AGGCTATCGC CACGGTAACC AGTCTGGTGC TGCAGATCGG CGAGGCTGGC 
CACAAAGGAC AAACATCGGG GTCGGAGGTC ATCAGCCAAG AGAAGGCGAA AGCGGCGGCA 
CCCCCTCAGG CTGAGGACAC CAGCGGTGAC GGGTGCTCGG AGAGCGAGTG GACGGTCAGC 
TATGCTCAGA TCCTGGCCTC ACTGCTCACT GAACAGGCGC TGGTGAACTT TTTCGAGAAG 
CCGGTGGATC TGACGGCGAA AATCGCAGAA GCCAAGGAGA GCCAGTATCA CCAGCGGGCG 
GGTTTGCTCA CTCTGCAACA AGGGACCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022057156.1
CDS	240..3632
Translation	MWLNPEEVLLKNALKLWVTERSNDFFLLQRRRGHGEPTSRITGLLVGALDSVLDSNARVTPFRILLQVPGSQISWVIASGAVIEEVNKHWDWLVHNLLHSLSVFENKEDVASFVKGKVKGLIAEEVRGRQAAQEDDPEKFREALLKFELHFGLPSSEKLVTYFSCCCWKGRVPRQGFLYLSINHMAFYSFLLGKEVKFVIPWAEVMRLERVSTGLMTEAIRVNTRQRQREFSMFLNMDEAFGVVSQLADIALRRLLDSEGLELDRVLQQPTRITKRILEEQAIREYVLALFRLPRAEKLYEVASCSVWTPHARCHTAGSLYTTDSYLCFSSREEGNCILLIPLSEVLSIEKAESTSLLPNPVIVSIRTKKAFQLIELQDRDELVESLNSRLRSMQWKQSMFRSRKDSKRSVSSPTPYYTFYYDTGFSDEEEIEEQVLRNTVNSEALMTAFHQNQPGTNGKKSAEEVKERLWEDHFTEFGRGVHMFRTEKIQRLVAMGIPESLRGELWMALSDASSELESHQGYYTSLVQKSMGHSSLATEEIERDLHRSLPDHPAFQNPTGIAALRRVLTAYAHRNPKIGYCQSMNILASVLLLYAKEEEAFWLLVAVCERMLPDYFNRRVIGAQVDQSVFEELIRERLPELAEQVPDLSTLSSVSLSWFLTLFLSVLPFQSAVCVVDCFFFHGIKAIFQLGLAVLDANAAQLSASTDDGQALMILTSFLDQVGGEDSSSVPSCPPAADESSCSDADVPAVRHTNITDLINESYEKFGDLTVRQIERLRCRHRIRVLQAHEDTTKENALRLVTPDVSISPENLADVYDLFKTEHFISLYWGDSSSAAAAEAAAWRYGDSGRSYVERQYRLDRPQFKSLYGLLVPWPGGSNQHTDTLANRTFTLLDQDRDNLVTFREFASWLDTLYCEELNEKIRLLYRLHIPPALTECEADPSLMKSPLLSTNRPLYVNLSSGDEDEVKDYQEQLKQMLQDLMKEKEKDVEKPLPLMNQREFIQFCKTLYSMFHSDPEENDLFQAIATVTSLVLQIGEAGHKGQTSGSEVISQEKAKAAAPPQAEDTSGDGCSESEWTVSYAQILASLLTEQALVNFFEKPVDLTAKIAEAKESQYHQRAGLLTLQQGTS

Target ORF information:

RefSeq Version	XM_022201464.1
Organism	Acanthochromis polyacanthus(spiny chromis)
Definition	Acanthochromis polyacanthus TBC1 domain family member 8 (tbc1d8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022201464.1

ORF Insert Sequence:

ATGTGGCTGA ACCCGGAGGA AGTTCTGCTG AAAAACGCTC TGAAGCTCTG GGTAACCGAG 
AGGAGCAACG ACTTCTTCCT GCTGCAGCGG AGGAGAGGCC ACGGAGAGCC CACCAGCAGG 
ATCACAGGGC TCCTGGTGGG GGCTCTGGAC TCAGTGCTGG ACTCCAATGC CAGGGTCACC 
CCGTTCCGAA TCCTGCTCCA GGTTCCTGGT TCCCAGATCA GCTGGGTCAT CGCTAGCGGA 
GCCGTCATCG AGGAGGTGAA CAAGCACTGG GACTGGCTGG TCCACAACCT GCTTCACTCG 
CTGTCGGTGT TTGAGAACAA AGAGGATGTT GCCAGCTTTG TCAAAGGAAA AGTGAAGGGC 
CTCATCGCAG AGGAGGTTCG GGGTCGCCAG GCCGCCCAGG AGGACGATCC GGAGAAGTTC 
AGGGAGGCGC TGCTGAAGTT TGAGCTGCAC TTCGGCCTCC CGTCATCGGA GAAGCTGGTG 
ACATACTTCT CCTGCTGCTG CTGGAAGGGC CGGGTGCCTC GACAGGGCTT CCTCTACCTC 
AGCATCAACC ATATGGCCTT CTACTCCTTC CTGCTGGGGA AGGAAGTTAA GTTTGTGATT 
CCATGGGCAG AGGTGATGCG GTTGGAGCGG GTCTCCACCG GCCTCATGAC CGAGGCGATC 
CGGGTGAACA CGCGGCAGCG ACAAAGAGAG TTCTCCATGT TCTTGAACAT GGACGAGGCG 
TTCGGGGTCG TCAGTCAGCT GGCTGACATT GCTCTGAGGC GGCTGCTGGA CAGCGAAGGC 
CTGGAGCTGG ACCGAGTCCT GCAGCAGCCC ACAAGGATCA CCAAGAGGAT TCTGGAGGAG 
CAGGCCATAA GGGAATACGT TCTCGCTCTG TTTCGACTTC CTCGTGCCGA GAAACTCTAC 
GAAGTGGCGT CCTGCTCGGT GTGGACGCCT CACGCTCGAT GCCATACGGC TGGAAGTCTG 
TACACCACCG ACAGCTACCT GTGTTTCTCC AGCAGAGAGG AAGGAAACTG TATCCTGCTC 
ATACCGCTGT CAGAGGTGTT GTCCATAGAA AAAGCAGAGA GCACCAGCCT CCTTCCCAAC 
CCGGTCATTG TGAGCATTCG GACTAAAAAG GCCTTCCAGC TGATTGAGCT GCAGGACAGA 
GATGAGCTGG TGGAAAGTCT GAACTCCAGA CTTCGATCTA TGCAGTGGAA ACAGTCTATG 
TTCCGCAGCA GGAAGGACAG CAAGAGGAGC GTGAGCTCTC CAACACCCTA CTACACTTTC 
TACTATGACA CTGGATTCTC TGATGAAGAA GAGATAGAGG AGCAGGTTCT ACGTAACACC 
GTCAACAGCG AAGCTCTGAT GACAGCTTTT CACCAAAACC AACCAGGAAC CAACGGCAAG 
AAGAGTGCTG AGGAGGTGAA GGAGCGGTTA TGGGAGGATC ACTTCACCGA GTTCGGTCGT 
GGCGTCCACA TGTTTCGCAC CGAGAAAATC CAAAGGCTGG TTGCTATGGG CATACCAGAG 
TCGCTGCGAG GGGAGCTGTG GATGGCACTT TCTGATGCAT CCTCAGAGCT GGAGTCTCAT 
CAGGGCTACT ACACCAGCCT GGTGCAGAAA TCGATGGGCC ACAGCAGTCT GGCCACAGAG 
GAGATAGAGA GGGACCTTCA CCGCTCGCTG CCGGACCATC CGGCCTTCCA GAACCCGACC 
GGCATTGCTG CACTCAGGCG TGTCCTGACC GCCTACGCTC ACCGCAACCC AAAGATTGGA 
TACTGTCAGT CTATGAACAT CCTGGCATCG GTGTTGCTGC TTTACGCCAA AGAAGAAGAA 
GCGTTTTGGC TGCTGGTGGC TGTTTGTGAG AGGATGCTGC CTGACTACTT CAACCGCAGG 
GTCATAGGAG CTCAGGTGGA TCAGTCGGTG TTTGAGGAGC TGATCAGGGA ACGTTTACCA 
GAGTTGGCCG AACAGGTTCC AGACCTCTCG ACTCTCTCCT CCGTCTCCCT CTCCTGGTTC 
CTCACCCTCT TCCTCAGTGT CCTGCCCTTC CAGAGCGCCG TCTGCGTGGT CGACTGCTTC 
TTCTTCCACG GAATCAAAGC CATCTTCCAG CTGGGTTTGG CTGTTCTGGA TGCCAACGCT 
GCTCAGCTCT CAGCAAGCAC AGACGATGGA CAGGCACTGA TGATTCTCAC AAGTTTCCTG 
GATCAGGTTG GAGGTGAGGA TTCGTCTTCT GTCCCGTCCT GTCCGCCTGC TGCAGATGAA 
AGCAGCTGCA GCGATGCTGA TGTTCCAGCT GTGAGACACA CAAACATCAC TGACCTCATC 
AACGAGTCCT ATGAGAAATT TGGAGATCTG ACAGTGCGGC AGATCGAGAG ACTTCGCTGT 
CGACACAGAA TCCGAGTACT GCAGGCTCAT GAAGACACCA CCAAAGAAAA TGCTTTAAGG 
CTGGTGACTC CAGATGTTTC CATCTCTCCA GAGAACCTGG CTGATGTTTA TGATCTCTTC 
AAGACGGAGC ACTTCATCAG TTTATATTGG GGCGACAGCA GCTCTGCAGC AGCAGCCGAG 
GCAGCAGCTT GGCGTTACGG AGACTCTGGT CGCTCCTACG TGGAGCGTCA GTATCGGCTG 
GACCGACCCC AGTTTAAAAG CCTGTACGGA CTGCTGGTCC CGTGGCCTGG AGGCTCAAAC 
CAGCACACTG ACACTCTGGC CAACCGAACC TTCACCTTGC TGGACCAAGA CCGAGACAAC 
CTGGTCACCT TTAGAGAGTT TGCCAGCTGG CTGGACACTT TGTACTGTGA AGAGCTGAAT 
GAGAAAATTC GGCTGCTGTA TCGGCTTCAC ATCCCTCCAG CTTTGACTGA ATGTGAGGCC 
GACCCCTCCC TGATGAAAAG CCCCCTGCTG TCCACAAACA GACCACTTTA TGTTAATCTG 
TCCTCTGGTG ATGAAGATGA AGTTAAAGAC TACCAGGAGC AGCTGAAGCA GATGCTGCAG 
GATCTGATGA AAGAAAAGGA GAAAGACGTA GAGAAGCCTT TGCCACTCAT GAACCAGCGG 
GAGTTCATCC AGTTCTGTAA AACGCTCTAC AGCATGTTCC ACAGTGACCC TGAGGAGAAT 
GACCTCTTTC AGGCTATCGC CACGGTAACC AGTCTGGTGC TGCAGATCGG CGAGGCTGGC 
CACAAAGGAC AAACATCGGG GTCGGAGGTC ATCAGCCAAG AGAAGGCGAA AGCGGCGGCA 
CCCCCTCAGG CTGAGGACAC CAGCGGTGAC GGGTGCTCGG AGAGCGAGTG GACGGTCAGC 
TATGCTCAGA TCCTGGCCTC ACTGCTCACT GAACAGGCGC TGGTGAACTT TTTCGAGAAG 
CCGGTGGATC TGACGGCGAA AATCGCAGAA GCCAAGGAGA GCCAGTATCA CCAGCGGGCG 
GGTTTGCTCA CTCTGCAACA AGGGACCAGC TGA