HEG1 cDNA ORF clone, Equus caballus(horse)

The following HEG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb53299 XM_023623607.1
Latest version!
Equus caballus heart development protein with EGF like domains 1 (HEG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb53300 XM_023623608.1
Latest version!
Equus caballus heart development protein with EGF like domains 1 (HEG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb09152 XM_001500006.3
Latest version!
Equus caballus heart development protein with EGF-like domains 1 (HEG1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb53299
    Clone ID Related Accession (Same CDS sequence) XM_023623607.1
    Accession Version XM_023623607.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product protein HEG homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009162.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCTCGC CGCGCGCCTC GCGGGGGCCG CCGCCGCTCC TGCTGCTGCT GCTGCTGCCG 
    CCGCTGCTGC TGCTGCTGCC GCCCGCCGCC CCGGCCCCCG GCGCGCCGAG CGGGACGCCC
    CTCGCCGGCG CGGGGCTGCA GGGGACGCCC GATGCGGGCC CCGAGAGCGA GCCGCCGCCG
    CCCACGCCGC CCGGCCCCCG CCGGCCGGAC CCGGGGCAGG ACGCCGCGAC ACGGCGGGGA
    CCCTCGGGCC CGGCCCCCCC AGGCGCGAGC GCGGCTACGG TAAGGGACGA CTCGCCAGAA
    AGTAACACTG ACCTGCACGT AGAAAACATC ACTGCTGATC ACAATCAAAT GAACTTTTCG
    ACAGCAGCCT CCAGCGAGGG TGTGGAGACT CAGACCCCCA GAGACAGCCA TGCTTCTTCC
    GATGCTCCAG GAAACTCCAC GGCACAACCC AAAACGGCAC GTGCTGAAGG AAGGAACAAC
    TCTTCAAGGA GAGCAGGTTT CACCGTTTCC TCTGTTGGGC CTGAGATAGC GACAGCTCTG
    ACTTCCCAGA GCGGCTCCTT GGCTTCTGAA AGTATGGAGG AGGTGAACAG CTCCACTGTG
    CTCCAGAGCC CCTCCGTCAG TCAGACAAAA CGAGTGCCTG TTGCCACCAC GTTCCCCGAT
    GGTGTCCCAA GGATGCTCCA GTCTTCAACC GTCAGTCTGG GACCTCTAAA CGAGACAGAG
    AGTTTCCCCG AGGACTCTGA AATTGCCACG ACTTCAGCAT CAGTCCATTC TTCACCCTCT
    GAGGCAGAAT CGAAAAGAAA CGATGAAGTC ATGGGGAATC CAGGGCATGG GGAGTTCACT
    GAGCCATTCA CAGAAAATGG ATTTGGCCTT ACATCTTCCA AGGTCTCAGT GGGACTTTGG
    CAAAATGATT CCCCGACCTC CAGAGGACAC CAGCTTGCCA GCAGCTCTGA GGCAGAGAAC
    AGAAGCCCCA TGTCTCCGAC TCAGACTGTG TCTAGGTCAC TCCCATTGGT CACAGATGGA
    GAAGGCACTG CACGCTGGTT CTCGACCGAC AGCAAGACAT TCACAGATGT GACAGGAAGC
    TCTACTTTCT ATCCTGATGT TGTGAACGCT TCGGATTTGA CTCAGTTCTC AGCCTCTGCC
    CCACAGTCTA GAGGAAGCGA CACAGCACTG GGTGATAGGG GCTACTCTGA GCCGGCCACC
    GAGACTTTGT CTTCACCTGC CTCAAAGAAC CAGAATTCCT CCTCACCACG TGGTGAACGC
    TCCACCACTG AAGACGGTCC TGAGCTGAGA GTCATTTCCG AGGCACAGGA GGGGACTTCC
    GAGGGGGCGA CTGGAGCCCG TGCTCCCAGC CCGCACACTT CAGCCACTTT CACTGGAAGC
    GGAGAGCGCA CGCTGAGGTC TCTGACCAAC GGGAGCACCA CTCCTGGGGA CGTGGGGCAC
    TCTGTGGCGG CGCCCAGAGA AACAGAGAGT GCCACCCAGC AGGGGAACGT GACCATGACT
    GATGACGCCC ACCTGGTCTC GGGTTCACCA GCGGCCTCGC CTGCCCTTGG AGTCACTGGA
    ATTGGCAGTC CCTGGAATCA AGTGAGTGGC ACAGACGTTG AAGAGAGAAC TTCCAGCGAC
    TACACAGGCC ACACCTATGT TTCGTCTACT TTCCCCAAAG GAGAATGGGC ATTACTGTCC
    ATCACAGACA ACAGCTCATC CTCGGACGTT AGGGAGAGTT CGACCTCTTC TATTAAGATC
    TCAAACTCTT CCTATTCAGA CTACCCTTCC TCTTCTCAGG CTCAGACTGA AAGAAGTAAT
    GTGTCATCCT ACGAAGGGGA ATATGCTCAG CCTTCTACTC ACTCCCTGCT TCTGCGCACA
    GCCAACCTTC CGTCCTATAC ACCCACCGTT AATATGTCAG ACCCTTTGGT GCTTCTGGAC
    AGTGATACTG GCTCGCTTGG TGACTCATCT TCTTCTTCAT CAGGGTCCCC TTTGCCTCTG
    CCCTCAGTGT CACAATCCCA CCAGTTGTTC TCATCCACCT TACCATCAAC CAGGGCCTCT
    GCTCACCCAC TGCAGTCCAC TCCTGGTGCG CCCATACCTT TGTCTTCCTC ACCACCACCT
    GTACCAATAT CTCTGACAGC ATCTACCCCT GCCTCGAAGT CCATCTTCCA AACAACCCTA
    CCGCCTTCAT CTTCTACACT CGTCCTGCCC AGGGCAAGGG ACACTCCTGT GACTTCAGTT
    TGGATGTCAA CAATGACATC TGTAACAATC CTCCCCAGTA GTCGAACAGC AGATCCTAAG
    AACCAGAGCA ACTCACACCA TGAGCAAATC ATTACGGAAT CAGAGCTACC AAGCCTGGAG
    TCTCTGCCCA CAGAGGCCAC CGAAGCTGTA ACAATGAGGT CTACTTCAGG GATCTCTATG
    TCCCCAGCCT CAACAGAGTC CTCTACTGAG CAGACTCTGC CAGCCACAAG CACCAGCGTA
    GCCCAAACGT CTCCAGCTTT GACAACCACC TATCTCGAAA CCTCCTCTCC CCCTGTGACC
    ACTCCCAGCC CCGCATCAAG CACAGCAGCT CTGACGGCTG GCCCCACAGT ACAGACTACA
    ACCAGAAAAC AGCTCTTGAC AACCAGTCCT GAAATTCCAG CACCTCCGAT CTCAACAGAA
    GGTTCTGTCA CCACAGAAAG GAACCAAGTC CGTATAGATG CTACCATCCG ATTGGTCCCC
    CTGACCAGTA CACCCACTTC GGCAGAAGAA CTGACCACGG GGGTTGGCAT TACAGAAGAG
    GACACCCCAA CTTCACATTT CCTCAGAACA TCTCCTTCTC CCCAAACCAC AGATGTCTCT
    ACAGCTAAAA TGTTGCCTCC TAAATCTACT GCCTCCACTG CTCGGAGCAG CACGCAGTCA
    CCAACAGCAT TGTCCTCTCC AGCCTCAGCT AACAGCTGTG CCACTAACCC TTGTCTTCAT
    GACGGGAAGT GCGTTGTGGA CCCCGCCAGC CGAGGGTATC AATGCGTGTG CTCACCTTCC
    TGGCAAGGAG ATGACTGCAG TGTAGATGTG AATGAATGCC TCTTGAACCC CTGCCCACCC
    CTGGCCACGT GCAACAATAC TCAGGGATCC TTCACCTGCA AATGCCCGGT TGGGTACCAG
    TTGGAAAAAG AAATATGCAA TTTGGTTAGA ACCTTCGTGA CAGATTTTAA ATTAAAGAAA
    ACATTTCTTA ATACCACAGT GGAAAAGTAT TCAGACCTAC GTGAAGCTGA AAAGGAGATC
    ACCAGAACAT TAAATCTGTG TTTCTCCACA TTACCTGGTT ATACCCGATC CACAGTTCAC
    GCTTCTAGGG AGTCCAGCGC GGTGGCCATG TCACTACAAA CCACCTTCTC CCTGGCCTCC
    AACGTGACGC TGTTCGACCT GGCTGACAGG ATGCAGAAGT GTGTCAACTC CTGCAGGTCC
    TCCGCGGAGG TCTGTCAGCT CTTGGGATCT CAGAAGAGGC TCTTTAGAGC GGGCAGCTTG
    TGCAAGCGGA AGACTCCGGA ATGTGACAAA GAGACCTCCA TCTGCACTGA TTTGGACGGC
    GTGGCGCTGT GTCAGTGCAA GTCGGGGTAC TTTCAGTTCA ACAAGATGGA TCACTCCTGC
    CGAGCATGTG AAGATGGATA CAGGCTTGAA AATGAAACCT GTATGAGTTG CCCATTCGGC
    CTTGGTGGTC TCAACTGTGG AAACCCCTAT CAGCTCATCA CCGTGGTGAT TGCAGCAGCC
    GGAGGCGGGC TCCTGCTCAT CCTGGGCATT GCACTGATTG TCACCTGTTG CAGAAAGAAT
    AAAAATGACA TCAGCAAACT CATCTTCAAA AGTGGGGATT TCCAAATGTC CCCATATGCT
    GAGTACCCCA AGAACCCTCG CTCACAAGAG TGGGGCCGAG AAGCCATTGA GATGCATGAG
    AATGGAAGCA CCAAAAACCT CCTCCAGATG ACAGATGTGT ATTACTCGCC CACAAGTGTA
    AGGAATCCTG AACTTGAACG AAATGGACTC TACCCGGCCT ACACTGGATT GCCGGGGTCA
    CGGCATTCTT GCATCTTCCC TGGACAGTAT AACCCATCTT TCATCAGTGA TGAGAGCAGG
    AGAAGAGACT ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023479375.1
    CDS16..4113
    Translation

    Target ORF information:

    RefSeq Version XM_023623607.1
    Organism Equus caballus(horse)
    Definition Equus caballus heart development protein with EGF like domains 1 (HEG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023623607.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCTCGC CGCGCGCCTC GCGGGGGCCG CCGCCGCTCC TGCTGCTGCT GCTGCTGCCG 
    CCGCTGCTGC TGCTGCTGCC GCCCGCCGCC CCGGCCCCCG GCGCGCCGAG CGGGACGCCC
    CTCGCCGGCG CGGGGCTGCA GGGGACGCCC GATGCGGGCC CCGAGAGCGA GCCGCCGCCG
    CCCACGCCGC CCGGCCCCCG CCGGCCGGAC CCGGGGCAGG ACGCCGCGAC ACGGCGGGGA
    CCCTCGGGCC CGGCCCCCCC AGGCGCGAGC GCGGCTACGG TAAGGGACGA CTCGCCAGAA
    AGTAACACTG ACCTGCACGT AGAAAACATC ACTGCTGATC ACAATCAAAT GAACTTTTCG
    ACAGCAGCCT CCAGCGAGGG TGTGGAGACT CAGACCCCCA GAGACAGCCA TGCTTCTTCC
    GATGCTCCAG GAAACTCCAC GGCACAACCC AAAACGGCAC GTGCTGAAGG AAGGAACAAC
    TCTTCAAGGA GAGCAGGTTT CACCGTTTCC TCTGTTGGGC CTGAGATAGC GACAGCTCTG
    ACTTCCCAGA GCGGCTCCTT GGCTTCTGAA AGTATGGAGG AGGTGAACAG CTCCACTGTG
    CTCCAGAGCC CCTCCGTCAG TCAGACAAAA CGAGTGCCTG TTGCCACCAC GTTCCCCGAT
    GGTGTCCCAA GGATGCTCCA GTCTTCAACC GTCAGTCTGG GACCTCTAAA CGAGACAGAG
    AGTTTCCCCG AGGACTCTGA AATTGCCACG ACTTCAGCAT CAGTCCATTC TTCACCCTCT
    GAGGCAGAAT CGAAAAGAAA CGATGAAGTC ATGGGGAATC CAGGGCATGG GGAGTTCACT
    GAGCCATTCA CAGAAAATGG ATTTGGCCTT ACATCTTCCA AGGTCTCAGT GGGACTTTGG
    CAAAATGATT CCCCGACCTC CAGAGGACAC CAGCTTGCCA GCAGCTCTGA GGCAGAGAAC
    AGAAGCCCCA TGTCTCCGAC TCAGACTGTG TCTAGGTCAC TCCCATTGGT CACAGATGGA
    GAAGGCACTG CACGCTGGTT CTCGACCGAC AGCAAGACAT TCACAGATGT GACAGGAAGC
    TCTACTTTCT ATCCTGATGT TGTGAACGCT TCGGATTTGA CTCAGTTCTC AGCCTCTGCC
    CCACAGTCTA GAGGAAGCGA CACAGCACTG GGTGATAGGG GCTACTCTGA GCCGGCCACC
    GAGACTTTGT CTTCACCTGC CTCAAAGAAC CAGAATTCCT CCTCACCACG TGGTGAACGC
    TCCACCACTG AAGACGGTCC TGAGCTGAGA GTCATTTCCG AGGCACAGGA GGGGACTTCC
    GAGGGGGCGA CTGGAGCCCG TGCTCCCAGC CCGCACACTT CAGCCACTTT CACTGGAAGC
    GGAGAGCGCA CGCTGAGGTC TCTGACCAAC GGGAGCACCA CTCCTGGGGA CGTGGGGCAC
    TCTGTGGCGG CGCCCAGAGA AACAGAGAGT GCCACCCAGC AGGGGAACGT GACCATGACT
    GATGACGCCC ACCTGGTCTC GGGTTCACCA GCGGCCTCGC CTGCCCTTGG AGTCACTGGA
    ATTGGCAGTC CCTGGAATCA AGTGAGTGGC ACAGACGTTG AAGAGAGAAC TTCCAGCGAC
    TACACAGGCC ACACCTATGT TTCGTCTACT TTCCCCAAAG GAGAATGGGC ATTACTGTCC
    ATCACAGACA ACAGCTCATC CTCGGACGTT AGGGAGAGTT CGACCTCTTC TATTAAGATC
    TCAAACTCTT CCTATTCAGA CTACCCTTCC TCTTCTCAGG CTCAGACTGA AAGAAGTAAT
    GTGTCATCCT ACGAAGGGGA ATATGCTCAG CCTTCTACTC ACTCCCTGCT TCTGCGCACA
    GCCAACCTTC CGTCCTATAC ACCCACCGTT AATATGTCAG ACCCTTTGGT GCTTCTGGAC
    AGTGATACTG GCTCGCTTGG TGACTCATCT TCTTCTTCAT CAGGGTCCCC TTTGCCTCTG
    CCCTCAGTGT CACAATCCCA CCAGTTGTTC TCATCCACCT TACCATCAAC CAGGGCCTCT
    GCTCACCCAC TGCAGTCCAC TCCTGGTGCG CCCATACCTT TGTCTTCCTC ACCACCACCT
    GTACCAATAT CTCTGACAGC ATCTACCCCT GCCTCGAAGT CCATCTTCCA AACAACCCTA
    CCGCCTTCAT CTTCTACACT CGTCCTGCCC AGGGCAAGGG ACACTCCTGT GACTTCAGTT
    TGGATGTCAA CAATGACATC TGTAACAATC CTCCCCAGTA GTCGAACAGC AGATCCTAAG
    AACCAGAGCA ACTCACACCA TGAGCAAATC ATTACGGAAT CAGAGCTACC AAGCCTGGAG
    TCTCTGCCCA CAGAGGCCAC CGAAGCTGTA ACAATGAGGT CTACTTCAGG GATCTCTATG
    TCCCCAGCCT CAACAGAGTC CTCTACTGAG CAGACTCTGC CAGCCACAAG CACCAGCGTA
    GCCCAAACGT CTCCAGCTTT GACAACCACC TATCTCGAAA CCTCCTCTCC CCCTGTGACC
    ACTCCCAGCC CCGCATCAAG CACAGCAGCT CTGACGGCTG GCCCCACAGT ACAGACTACA
    ACCAGAAAAC AGCTCTTGAC AACCAGTCCT GAAATTCCAG CACCTCCGAT CTCAACAGAA
    GGTTCTGTCA CCACAGAAAG GAACCAAGTC CGTATAGATG CTACCATCCG ATTGGTCCCC
    CTGACCAGTA CACCCACTTC GGCAGAAGAA CTGACCACGG GGGTTGGCAT TACAGAAGAG
    GACACCCCAA CTTCACATTT CCTCAGAACA TCTCCTTCTC CCCAAACCAC AGATGTCTCT
    ACAGCTAAAA TGTTGCCTCC TAAATCTACT GCCTCCACTG CTCGGAGCAG CACGCAGTCA
    CCAACAGCAT TGTCCTCTCC AGCCTCAGCT AACAGCTGTG CCACTAACCC TTGTCTTCAT
    GACGGGAAGT GCGTTGTGGA CCCCGCCAGC CGAGGGTATC AATGCGTGTG CTCACCTTCC
    TGGCAAGGAG ATGACTGCAG TGTAGATGTG AATGAATGCC TCTTGAACCC CTGCCCACCC
    CTGGCCACGT GCAACAATAC TCAGGGATCC TTCACCTGCA AATGCCCGGT TGGGTACCAG
    TTGGAAAAAG AAATATGCAA TTTGGTTAGA ACCTTCGTGA CAGATTTTAA ATTAAAGAAA
    ACATTTCTTA ATACCACAGT GGAAAAGTAT TCAGACCTAC GTGAAGCTGA AAAGGAGATC
    ACCAGAACAT TAAATCTGTG TTTCTCCACA TTACCTGGTT ATACCCGATC CACAGTTCAC
    GCTTCTAGGG AGTCCAGCGC GGTGGCCATG TCACTACAAA CCACCTTCTC CCTGGCCTCC
    AACGTGACGC TGTTCGACCT GGCTGACAGG ATGCAGAAGT GTGTCAACTC CTGCAGGTCC
    TCCGCGGAGG TCTGTCAGCT CTTGGGATCT CAGAAGAGGC TCTTTAGAGC GGGCAGCTTG
    TGCAAGCGGA AGACTCCGGA ATGTGACAAA GAGACCTCCA TCTGCACTGA TTTGGACGGC
    GTGGCGCTGT GTCAGTGCAA GTCGGGGTAC TTTCAGTTCA ACAAGATGGA TCACTCCTGC
    CGAGCATGTG AAGATGGATA CAGGCTTGAA AATGAAACCT GTATGAGTTG CCCATTCGGC
    CTTGGTGGTC TCAACTGTGG AAACCCCTAT CAGCTCATCA CCGTGGTGAT TGCAGCAGCC
    GGAGGCGGGC TCCTGCTCAT CCTGGGCATT GCACTGATTG TCACCTGTTG CAGAAAGAAT
    AAAAATGACA TCAGCAAACT CATCTTCAAA AGTGGGGATT TCCAAATGTC CCCATATGCT
    GAGTACCCCA AGAACCCTCG CTCACAAGAG TGGGGCCGAG AAGCCATTGA GATGCATGAG
    AATGGAAGCA CCAAAAACCT CCTCCAGATG ACAGATGTGT ATTACTCGCC CACAAGTGTA
    AGGAATCCTG AACTTGAACG AAATGGACTC TACCCGGCCT ACACTGGATT GCCGGGGTCA
    CGGCATTCTT GCATCTTCCC TGGACAGTAT AACCCATCTT TCATCAGTGA TGAGAGCAGG
    AGAAGAGACT ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb53300
    Clone ID Related Accession (Same CDS sequence) XM_023623608.1
    Accession Version XM_023623608.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product protein HEG homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009162.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCTCGC CGCGCGCCTC GCGGGGGCCG CCGCCGCTCC TGCTGCTGCT GCTGCTGCCG 
    CCGCTGCTGC TGCTGCTGCC GCCCGCCGCC CCGGCCCCCG GCGCGCCGAG CGGGACGCCC
    CTCGCCGGCG CGGGGCTGCA GGGGACGCCC GATGCGGGCC CCGAGAGCGA GCCGCCGCCG
    CCCACGCCGC CCGGCCCCCG CCGGCCGGAC CCGGGGCAGG ACGCCGCGAC ACGGCGGGGA
    CCCTCGGGCC CGGCCCCCCC AGGCGCGAGC GCGGCTACGG TAAGGGACGA CTCGCCAGAA
    AGTAACACTG ACCTGCACGT AGAAAACATC ACTGCTGATC ACAATCAAAT GAACTTTTCG
    ACAGCAGCCT CCAGCGAGGG TGTGGAGACT CAGACCCCCA GAGACAGCCA TGCTTCTTCC
    GATGCTCCAG GAAACTCCAC GGCACAACCC AAAACGGCAC GTGCTGAAGG AAGGAACAAC
    TCTTCAAGGA GAGCAGGTTT CACCGTTTCC TCTGTTGGGC CTGAGATAGC GACAGCTCTG
    ACTTCCCAGA GCGGCTCCTT GGCTTCTGAA AGTATGGAGG AGGTGAACAG CTCCACTGTG
    CTCCAGAGCC CCTCCGTCAG TCAGACAAAA CGAGTGCCTG TTGCCACCAC GTTCCCCGAT
    GGTGTCCCAA GGATGCTCCA GTCTTCAACC GTCAGTCTGG GACCTCTAAA CGAGACAGAG
    AGTTTCCCCG AGGACTCTGA AATTGCCACG ACTTCAGCAT CAGTCCATTC TTCACCCTCT
    GAGGCAGAAT CGAAAAGAAA CGATGAAGTC ATGGGGAATC CAGGGCATGG GGAGTTCACT
    GAGCCATTCA CAGAAAATGG ATTTGGCCTT ACATCTTCCA AGGTCTCAGT GGGACTTTGG
    CAAAATGATT CCCCGACCTC CAGAGGACAC CAGCTTGCCA GCAGCTCTGA GGCAGAGAAC
    AGAAGCCCCA TGTCTCCGAC TCAGACTGTG TCTAGGTCAC TCCCATTGGT CACAGATGGA
    GAAGGCACTG CACGCTGGTT CTCGACCGAC AGCAAGACAT TCACAGATGT GACAGGAAGC
    TCTACTTTCT ATCCTGATGT TGTGAACGCT TCGGATTTGA CTCAGTTCTC AGCCTCTGCC
    CCACAGTCTA GAGGAAGCGA CACAGCACTG GGTGATAGGG GCTACTCTGA GCCGGCCACC
    GAGACTTTGT CTTCACCTGC CTCAAAGAAC CAGAATTCCT CCTCACCACG TGGTGAACGC
    TCCACCACTG AAGACGGTCC TGAGCTGAGA GTCATTTCCG AGGCACAGGA GGGGACTTCC
    GAGGGGGCGA CTGGAGCCCG TGCTCCCAGC CCGCACACTT CAGCCACTTT CACTGGAAGC
    GGAGAGCGCA CGCTGAGGTC TCTGACCAAC GGGAGCACCA CTCCTGGGGA CGTGGGGCAC
    TCTGTGGCGG CGCCCAGAGA AACAGAGAGT GCCACCCAGC AGGGGAACGT GACCATGACT
    GATGACGCCC ACCTGGTCTC GGGTTCACCA GCGGCCTCGC CTGCCCTTGG AGTCACTGGA
    ATTGGCAGTC CCTGGAATCA AGTGAGTGGC ACAGACGTTG AAGAGAGAAC TTCCAGCGAC
    TACACAGGCC ACACCTATGT TTCGTCTACT TTCCCCAAAG GAGAATGGGC ATTACTGTCC
    ATCACAGACA ACAGCTCATC CTCGGACGTT AGGGAGAGTT CGACCTCTTC TATTAAGATC
    TCAAACTCTT CCTATTCAGA CTACCCTTCC TCTTCTCAGG CTCAGACTGA AAGAAGTAAT
    GTGTCATCCT ACGAAGGGGA ATATGCTCAG CCTTCTACTC ACTCCCTGCT TCTGCGCACA
    GCCAACCTTC CGTCCTATAC ACCCACCGTT AATATGTCAG ACCCTTTGGT GCTTCTGGAC
    AGTGATACTG GCTCGCTTGG TGACTCATCT TCTTCTTCAT CAGGGTCCCC TTTGCCTCTG
    CCCTCAGTGT CACAATCCCA CCAGTTGTTC TCATCCACCT TACCATCAAC CAGGGCCTCT
    GCTCACCCAC TGCAGTCCAC TCCTGGTGCG CCCATACCTT TGTCTTCCTC ACCACCACCT
    GTACCAATAT CTCTGACAGC ATCTACCCCT GCCTCGAAGT CCATCTTCCA AACAACCCTA
    CCGCCTTCAT CTTCTACACT CGTCCTGCCC AGGGCAAGGG ACACTCCTGT GACTTCAGTT
    TGGATGTCAA CAATGACATC TGTAACAATC CTCCCCAGTA GTCGAACAGC AGATCCTAAG
    AACCAGAGCA ACTCACACCA TGAGCAAATC ATTACGGAAT CAGAGCTACC AAGCCTGGAG
    TCTCTGCCCA CAGAGGCCAC CGAAGCTGTA ACAATGAGGT CTACTTCAGG GATCTCTATG
    TCCCCAGCCT CAACAGAGTC CTCTACTGAG CAGACTCTGC CAGCCACAAG CACCAGCGTA
    GCCCAAACGT CTCCAGCTTT GACAACCACC TATCTCGAAA CCTCCTCTCC CCCTGTGACC
    ACTCCCAGCC CCGCATCAAG CACAGCAGCT CTGACGGCTG GCCCCACAGT ACAGACTACA
    ACCAGAAAAC AGCTCTTGAC AACCAGTCCT GAAATTCCAG CACCTCCGAT CTCAACAGAA
    GGTTCTGTCA CCACAGAAAG GAACCAAGTC CGTATAGATG CTACCATCCG ATTGGTCCCC
    CTGACCAGTA CACCCACTTC GGCAGAAGAA CTGACCACGG GGGTTGGCAT TACAGAAGAG
    GACACCCCAA CTTCACATTT CCTCAGAACA TCTCCTTCTC CCCAAACCAC AGATGTCTCT
    ACAGCTAAAA TGTTGCCTCC TAAATCTACT GCCTCCACTG CTCGGAGCAG CACGCAGTCA
    CCAACAGCAT TGTCCTCTCC AGCCTCAGAT GTGAATGAAT GCCTCTTGAA CCCCTGCCCA
    CCCCTGGCCA CGTGCAACAA TACTCAGGGA TCCTTCACCT GCAAATGCCC GGTTGGGTAC
    CAGTTGGAAA AAGAAATATG CAATTTGGTT AGAACCTTCG TGACAGATTT TAAATTAAAG
    AAAACATTTC TTAATACCAC AGTGGAAAAG TATTCAGACC TACGTGAAGC TGAAAAGGAG
    ATCACCAGAA CATTAAATCT GTGTTTCTCC ACATTACCTG GTTATACCCG ATCCACAGTT
    CACGCTTCTA GGGAGTCCAG CGCGGTGGCC ATGTCACTAC AAACCACCTT CTCCCTGGCC
    TCCAACGTGA CGCTGTTCGA CCTGGCTGAC AGGATGCAGA AGTGTGTCAA CTCCTGCAGG
    TCCTCCGCGG AGGTCTGTCA GCTCTTGGGA TCTCAGAAGA GGCTCTTTAG AGCGGGCAGC
    TTGTGCAAGC GGAAGACTCC GGAATGTGAC AAAGAGACCT CCATCTGCAC TGATTTGGAC
    GGCGTGGCGC TGTGTCAGTG CAAGTCGGGG TACTTTCAGT TCAACAAGAT GGATCACTCC
    TGCCGAGCAT GTGAAGATGG ATACAGGCTT GAAAATGAAA CCTGTATGAG TTGCCCATTC
    GGCCTTGGTG GTCTCAACTG TGGAAACCCC TATCAGCTCA TCACCGTGGT GATTGCAGCA
    GCCGGAGGCG GGCTCCTGCT CATCCTGGGC ATTGCACTGA TTGTCACCTG TTGCAGAAAG
    AATAAAAATG ACATCAGCAA ACTCATCTTC AAAAGTGGGG ATTTCCAAAT GTCCCCATAT
    GCTGAGTACC CCAAGAACCC TCGCTCACAA GAGTGGGGCC GAGAAGCCAT TGAGATGCAT
    GAGAATGGAA GCACCAAAAA CCTCCTCCAG ATGACAGATG TGTATTACTC GCCCACAAGT
    GTAAGGAATC CTGAACTTGA ACGAAATGGA CTCTACCCGG CCTACACTGG ATTGCCGGGG
    TCACGGCATT CTTGCATCTT CCCTGGACAG TATAACCCAT CTTTCATCAG TGATGAGAGC
    AGGAGAAGAG ACTACTTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023479376.1
    CDS16..3996
    Translation

    Target ORF information:

    RefSeq Version XM_023623608.1
    Organism Equus caballus(horse)
    Definition Equus caballus heart development protein with EGF like domains 1 (HEG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023623608.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCTCGC CGCGCGCCTC GCGGGGGCCG CCGCCGCTCC TGCTGCTGCT GCTGCTGCCG 
    CCGCTGCTGC TGCTGCTGCC GCCCGCCGCC CCGGCCCCCG GCGCGCCGAG CGGGACGCCC
    CTCGCCGGCG CGGGGCTGCA GGGGACGCCC GATGCGGGCC CCGAGAGCGA GCCGCCGCCG
    CCCACGCCGC CCGGCCCCCG CCGGCCGGAC CCGGGGCAGG ACGCCGCGAC ACGGCGGGGA
    CCCTCGGGCC CGGCCCCCCC AGGCGCGAGC GCGGCTACGG TAAGGGACGA CTCGCCAGAA
    AGTAACACTG ACCTGCACGT AGAAAACATC ACTGCTGATC ACAATCAAAT GAACTTTTCG
    ACAGCAGCCT CCAGCGAGGG TGTGGAGACT CAGACCCCCA GAGACAGCCA TGCTTCTTCC
    GATGCTCCAG GAAACTCCAC GGCACAACCC AAAACGGCAC GTGCTGAAGG AAGGAACAAC
    TCTTCAAGGA GAGCAGGTTT CACCGTTTCC TCTGTTGGGC CTGAGATAGC GACAGCTCTG
    ACTTCCCAGA GCGGCTCCTT GGCTTCTGAA AGTATGGAGG AGGTGAACAG CTCCACTGTG
    CTCCAGAGCC CCTCCGTCAG TCAGACAAAA CGAGTGCCTG TTGCCACCAC GTTCCCCGAT
    GGTGTCCCAA GGATGCTCCA GTCTTCAACC GTCAGTCTGG GACCTCTAAA CGAGACAGAG
    AGTTTCCCCG AGGACTCTGA AATTGCCACG ACTTCAGCAT CAGTCCATTC TTCACCCTCT
    GAGGCAGAAT CGAAAAGAAA CGATGAAGTC ATGGGGAATC CAGGGCATGG GGAGTTCACT
    GAGCCATTCA CAGAAAATGG ATTTGGCCTT ACATCTTCCA AGGTCTCAGT GGGACTTTGG
    CAAAATGATT CCCCGACCTC CAGAGGACAC CAGCTTGCCA GCAGCTCTGA GGCAGAGAAC
    AGAAGCCCCA TGTCTCCGAC TCAGACTGTG TCTAGGTCAC TCCCATTGGT CACAGATGGA
    GAAGGCACTG CACGCTGGTT CTCGACCGAC AGCAAGACAT TCACAGATGT GACAGGAAGC
    TCTACTTTCT ATCCTGATGT TGTGAACGCT TCGGATTTGA CTCAGTTCTC AGCCTCTGCC
    CCACAGTCTA GAGGAAGCGA CACAGCACTG GGTGATAGGG GCTACTCTGA GCCGGCCACC
    GAGACTTTGT CTTCACCTGC CTCAAAGAAC CAGAATTCCT CCTCACCACG TGGTGAACGC
    TCCACCACTG AAGACGGTCC TGAGCTGAGA GTCATTTCCG AGGCACAGGA GGGGACTTCC
    GAGGGGGCGA CTGGAGCCCG TGCTCCCAGC CCGCACACTT CAGCCACTTT CACTGGAAGC
    GGAGAGCGCA CGCTGAGGTC TCTGACCAAC GGGAGCACCA CTCCTGGGGA CGTGGGGCAC
    TCTGTGGCGG CGCCCAGAGA AACAGAGAGT GCCACCCAGC AGGGGAACGT GACCATGACT
    GATGACGCCC ACCTGGTCTC GGGTTCACCA GCGGCCTCGC CTGCCCTTGG AGTCACTGGA
    ATTGGCAGTC CCTGGAATCA AGTGAGTGGC ACAGACGTTG AAGAGAGAAC TTCCAGCGAC
    TACACAGGCC ACACCTATGT TTCGTCTACT TTCCCCAAAG GAGAATGGGC ATTACTGTCC
    ATCACAGACA ACAGCTCATC CTCGGACGTT AGGGAGAGTT CGACCTCTTC TATTAAGATC
    TCAAACTCTT CCTATTCAGA CTACCCTTCC TCTTCTCAGG CTCAGACTGA AAGAAGTAAT
    GTGTCATCCT ACGAAGGGGA ATATGCTCAG CCTTCTACTC ACTCCCTGCT TCTGCGCACA
    GCCAACCTTC CGTCCTATAC ACCCACCGTT AATATGTCAG ACCCTTTGGT GCTTCTGGAC
    AGTGATACTG GCTCGCTTGG TGACTCATCT TCTTCTTCAT CAGGGTCCCC TTTGCCTCTG
    CCCTCAGTGT CACAATCCCA CCAGTTGTTC TCATCCACCT TACCATCAAC CAGGGCCTCT
    GCTCACCCAC TGCAGTCCAC TCCTGGTGCG CCCATACCTT TGTCTTCCTC ACCACCACCT
    GTACCAATAT CTCTGACAGC ATCTACCCCT GCCTCGAAGT CCATCTTCCA AACAACCCTA
    CCGCCTTCAT CTTCTACACT CGTCCTGCCC AGGGCAAGGG ACACTCCTGT GACTTCAGTT
    TGGATGTCAA CAATGACATC TGTAACAATC CTCCCCAGTA GTCGAACAGC AGATCCTAAG
    AACCAGAGCA ACTCACACCA TGAGCAAATC ATTACGGAAT CAGAGCTACC AAGCCTGGAG
    TCTCTGCCCA CAGAGGCCAC CGAAGCTGTA ACAATGAGGT CTACTTCAGG GATCTCTATG
    TCCCCAGCCT CAACAGAGTC CTCTACTGAG CAGACTCTGC CAGCCACAAG CACCAGCGTA
    GCCCAAACGT CTCCAGCTTT GACAACCACC TATCTCGAAA CCTCCTCTCC CCCTGTGACC
    ACTCCCAGCC CCGCATCAAG CACAGCAGCT CTGACGGCTG GCCCCACAGT ACAGACTACA
    ACCAGAAAAC AGCTCTTGAC AACCAGTCCT GAAATTCCAG CACCTCCGAT CTCAACAGAA
    GGTTCTGTCA CCACAGAAAG GAACCAAGTC CGTATAGATG CTACCATCCG ATTGGTCCCC
    CTGACCAGTA CACCCACTTC GGCAGAAGAA CTGACCACGG GGGTTGGCAT TACAGAAGAG
    GACACCCCAA CTTCACATTT CCTCAGAACA TCTCCTTCTC CCCAAACCAC AGATGTCTCT
    ACAGCTAAAA TGTTGCCTCC TAAATCTACT GCCTCCACTG CTCGGAGCAG CACGCAGTCA
    CCAACAGCAT TGTCCTCTCC AGCCTCAGAT GTGAATGAAT GCCTCTTGAA CCCCTGCCCA
    CCCCTGGCCA CGTGCAACAA TACTCAGGGA TCCTTCACCT GCAAATGCCC GGTTGGGTAC
    CAGTTGGAAA AAGAAATATG CAATTTGGTT AGAACCTTCG TGACAGATTT TAAATTAAAG
    AAAACATTTC TTAATACCAC AGTGGAAAAG TATTCAGACC TACGTGAAGC TGAAAAGGAG
    ATCACCAGAA CATTAAATCT GTGTTTCTCC ACATTACCTG GTTATACCCG ATCCACAGTT
    CACGCTTCTA GGGAGTCCAG CGCGGTGGCC ATGTCACTAC AAACCACCTT CTCCCTGGCC
    TCCAACGTGA CGCTGTTCGA CCTGGCTGAC AGGATGCAGA AGTGTGTCAA CTCCTGCAGG
    TCCTCCGCGG AGGTCTGTCA GCTCTTGGGA TCTCAGAAGA GGCTCTTTAG AGCGGGCAGC
    TTGTGCAAGC GGAAGACTCC GGAATGTGAC AAAGAGACCT CCATCTGCAC TGATTTGGAC
    GGCGTGGCGC TGTGTCAGTG CAAGTCGGGG TACTTTCAGT TCAACAAGAT GGATCACTCC
    TGCCGAGCAT GTGAAGATGG ATACAGGCTT GAAAATGAAA CCTGTATGAG TTGCCCATTC
    GGCCTTGGTG GTCTCAACTG TGGAAACCCC TATCAGCTCA TCACCGTGGT GATTGCAGCA
    GCCGGAGGCG GGCTCCTGCT CATCCTGGGC ATTGCACTGA TTGTCACCTG TTGCAGAAAG
    AATAAAAATG ACATCAGCAA ACTCATCTTC AAAAGTGGGG ATTTCCAAAT GTCCCCATAT
    GCTGAGTACC CCAAGAACCC TCGCTCACAA GAGTGGGGCC GAGAAGCCAT TGAGATGCAT
    GAGAATGGAA GCACCAAAAA CCTCCTCCAG ATGACAGATG TGTATTACTC GCCCACAAGT
    GTAAGGAATC CTGAACTTGA ACGAAATGGA CTCTACCCGG CCTACACTGG ATTGCCGGGG
    TCACGGCATT CTTGCATCTT CCCTGGACAG TATAACCCAT CTTTCATCAG TGATGAGAGC
    AGGAGAAGAG ACTACTTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb09152
    Clone ID Related Accession (Same CDS sequence) XM_001500006.3
    Accession Version XM_001500006.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product protein HEG homolog 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867385.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001500006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAATCT GCATTTGTGT CTGCAGTACC ACGTGTAACC AGGCTACGGT AAGGGACGAC 
    TCGCCAGAAA GTAACACTGA CCTGCACGTA GAAAACATCA CTGCTGATCA CAATCAAATG
    AACTTTTCGA CAGCAGCCTC CAGCGAGGGT GTGGAGACTC AGACCCCCAG CGACAGCCAT
    GCTTCTTCCG ATGCTCCAGG AAACTCCACG GCACAACCCA AAACGGCACG TGCTGAAGGA
    AGGAACAACT CTTCAAGGAG AGCAGGTTTC ACCGTTTCCT CTGTTGGGCC TGAGATAGCG
    ACAGCTCTGA CTTCCCAGAG CGGCTCCTTG GCTTCTGAAA GTATGGAGGA GGTGAACAGC
    TCCACTGTGC TCCAGAGCCC CTCCGTCAGT CAGACAAAAC GAGTGCCTGT TGCCACCACG
    TTCCCCGATG GTGTCCCAAG GATGCTCCAG TCTTCAACCG TCAGTCTGGG ACCTCTAAAC
    GAGACAGAGA GTTTCCCCGA GGACTCTGAA ATTGCCACGA CTTCAGCATC AGTCCATTCT
    TCACCCTCTG AGGCAGAATC GAAAAGAAAC GATGAAGTCA TGGGGAATCC AGGGCATGGG
    GAGTTCACTG AGCCATTCAC AGAAAATGGA TTTGGCCTTA CATCTTCCAA GGTCTCAGTG
    GGACTTTGGC AAAATGATTC CCCGACCTCC AGAGGACACC AGCTTGCCAG CAGCTCTGAG
    GCAGAGAACA GAAGCCCCAT GTCTCCGACT CAGACTGTGT CTAGGTCACT CCCATTGGTC
    ACAGATGGAG AAGGCACTGC ACGCTGGTTC TCGACCGACA GCAAGACATT CACAGATGTG
    ACAGGAAGCT CTACTTTCTA TCCTGATGTT GTGAACGCTT CGGATTTGAC TCAGTTCTCA
    GCCTCTGCCC CACAGTCTAG AGGAAGCGAC ACAGCACTGG GTGATAGGGG CTACTCTGAG
    CCGGCCACCG AGACTTTGTC TTCACCTGCC TCAAAGAACC AGAATTCCTC CTCACCACGT
    GGTGAACGCT CCACCACTGA AGACGGTCCT GAGCTGAGAG TCATTTCCGA GGCACAGGAG
    GGGACTTCCG AGGGGGCGAC TGGAGCCCGT GCTCCCAGCC CGCACACTTC AGCCACTTTC
    ACTGGAAGCG GAGAGCGCAC GCTGAGGTCT CTGACCAACG GGAGCACCAC TCCTGGGGAC
    GTGGGGCACT CTGTGGCGGC GCCCAGAGAA ACAGAGAGTG CCACCCAGCA GGGGAACGTG
    ACCATGACTG ATGACGCCCA CCTGGTCTCG GGTTCACCAG CGGCCTCGCC TGCCCTTGGA
    GTCACTGGAA TTGGCAGTCC CTGGAATCAA GTGAGTGGCA CAGACGTTGA AGAGAGAACT
    TCCAGCGACT ACACAGGCCA CACCTATGTT TCGTCTACTT TCCCCAAAGG AGAATGGGCA
    TTACTGTCCA TCACAGACAA CAGCTCATCC TCGGACGTTA GGGAGAGTTC GACCTCTTCT
    ATTAAGATCT CAAACTCTTC CTATTCAGAC TACCCTTCCT CTTCTCAGGC TCAGACTGAA
    AGAAGTAATG TGTCATCCTA CGAAGGGGAA TATGCTCAGC CTTCTACTCA CTCCCTGCTT
    CTGCGCACAG CCAACCTTCC GTCCTATACA CCCACCGTTA ATATGTCAGA CCCTTTGGTG
    CTTCTGGACA GTGATACTGG CTCGCTTGGT GACTCATCTT CTTCTTCATC AGGGTCCCCT
    TTGCCTCTGC CCTCAGTGTC ACAATCCCAC CAGTTGTTCT CATCCACCTT ACCATCAACC
    AGGGCCTCTG CTCACCCACT GCAGTCCACT CCTGGTGCGC CCATACCTTT GTCTTCCTCA
    CCACCACCTG TACCAATATC TCTGACAGCA TCTACCCCTG CCTCGAAGTC CATCTTCCAA
    ACAACCCTAC CGCCTTCATC TTCTACACTC GTCCTGCCCA GGGCAAGGGA CACTCCTGTG
    ACTTCAGTTT GGATGTCAAC AATGACATCT GTAACAATCC TCCCCAGTAG TCGAACAGCA
    GATCCTAAGA ACCAGAGCAA CTCACACCAT GAGCAAATCA TTACGGAATC AGAGCTACCA
    AGCCTGGAGT CTCTGCCCAC AGAGGCCACC GAAGCTGTAA CAATGAGGTC TACTTCAGGG
    ATCTCTATGT CCCCAGCCTC AACAGAGTCC TCTACTGAGC AGACTCTGCC AGCCACAAGC
    ACCAGCGTAG CCCAAACGTC TCCAGCTTTG ACAACCACCT ATCTCGAAAC CTCCTCTCCC
    CCTGTGACCA CTCCCAGCCC CGCATCAAGC ACAGCAGCTC TGACGGCTGG CCCCACAGTA
    CAGACTACAA CCAGAAAACA GCTCTTGACA ACCAGTCCTG AAATTCCAGC ACCTCCGATC
    TCAACAGAAG GTTCTGTCAC CACAGAAAGG AACCAAGTCC GTATAGATGC TACCATCCGA
    TTGGTCCCCC TGACCAGTAC ACCCACTTCG GCAGAAGAAC TGACCACGGG GGTTGGCATT
    ACAGAAGAGG ACACCCCAAC TTCACATTTC CTCAGAACAT CTCCTTCTCC CCAAACCACA
    GATGTCTCTA CAGCTAAAAT GTTGCCTCCT AAATCTACTG CCTCCACTGC TCGGAGCAGC
    ACGCAGTCAC CAACAGCATT GTCCTCTCCA GCCTCAGCTA ACAGCTGTGC CACTAACCCT
    TGTCTTCATG ACGGGAAGTG CGTTGTGGAC CCCGCCAGCC GAGGGTATCA ATGCGTGTGC
    TCACCTTCCT GGCAAGGAGA TGACTGCAGT GTAGATGTGA ATGAATGCCT CTTGAACCCC
    TGCCCACCCC TGGCCACGTG CAACAATACT CAGGGATCCT TCACCTGCAA ATGCCCGGTT
    GGGTACCAGT TGGAAAAAGA AATATGCAAT TTGGTTAGAA CCTTCGTGAC AGATTTTAAA
    TTAAAGAAAA CATTTCTTAA TACCACAGTG GAAAAGTATT CAGACCTACG TGAAGCTGAA
    AAGGAGATCA CCAGAACATT AAATCTGTGT TTCTCCACAT TACCTGGTTA TACCCGATCC
    ACAGTTCACG CTTCTAGGGA GTCCAGCGCG GTGGCCATGT CACTACAAAC CACCTTCTCC
    CTGGCCTCCA ACGTGACGCT GTTCGACCTG GCTGACAGGA TGCAGAAGTG TGTCAACTCC
    TGCAGGTCCT CCGCGGAGGT CTGTCAGCTC TTGGGATCTC AGAAGAGGCT CTTTAGAGCG
    GGCAGCTTGT GCAAGCGGAA GACTCCGGAA TGTGACAAAG AGACCTCCAT CTGCACTGAT
    TTGGACGGCG TGGCGCTGTG TCAGTGCAAG TCGGGGTACT TTCAGTTCAA CAAGATGGAT
    CACTCCTGCC GAGCATGTGA AGATGGATAC AGGCTTGAAA ATGAAACCTG TATGAGTTGC
    CCATTCGGCC TTGGTGGTCT CAACTGTGGA AACCCCTATC AGCTCATCAC CGTGGTGATT
    GCAGCAGCCG GAGGCGGGCT CCTGCTCATC CTGGGCATTG CACTGATTGT CACCTGTTGC
    AGAAAGAATA AAAATGACAT CAGCAAACTC ATCTTCAAAA GTGGGGATTT CCAAATGTCC
    CCATATGCTG AGTACCCCAA GAACCCTCGC TCACAAGAGT GGGGCCGAGA AGCCATTGAG
    ATGCATGAGA ATGGAAGCAC CAAAAACCTC CTCCAGATGA CAGATGTGTA TTACTCGCCC
    ACAAGTGTAA GGAATCCTGA ACTTGAACGA AATGGACTCT ACCCGGCCTA CACTGGATTG
    CCGGGGTCAC GGCATTCTTG CATCTTCCCT GGACAGTATA ACCCATCTTT CATCAGTGAT
    GAGAGCAGGA GAAGAGACTA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001500056.2
    CDS1..3867
    Translation

    Target ORF information:

    RefSeq Version XM_001500006.3
    Organism Equus caballus(horse)
    Definition Equus caballus heart development protein with EGF-like domains 1 (HEG1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001500006.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAATCT GCATTTGTGT CTGCAGTACC ACGTGTAACC AGGCTACGGT AAGGGACGAC 
    TCGCCAGAAA GTAACACTGA CCTGCACGTA GAAAACATCA CTGCTGATCA CAATCAAATG
    AACTTTTCGA CAGCAGCCTC CAGCGAGGGT GTGGAGACTC AGACCCCCAG CGACAGCCAT
    GCTTCTTCCG ATGCTCCAGG AAACTCCACG GCACAACCCA AAACGGCACG TGCTGAAGGA
    AGGAACAACT CTTCAAGGAG AGCAGGTTTC ACCGTTTCCT CTGTTGGGCC TGAGATAGCG
    ACAGCTCTGA CTTCCCAGAG CGGCTCCTTG GCTTCTGAAA GTATGGAGGA GGTGAACAGC
    TCCACTGTGC TCCAGAGCCC CTCCGTCAGT CAGACAAAAC GAGTGCCTGT TGCCACCACG
    TTCCCCGATG GTGTCCCAAG GATGCTCCAG TCTTCAACCG TCAGTCTGGG ACCTCTAAAC
    GAGACAGAGA GTTTCCCCGA GGACTCTGAA ATTGCCACGA CTTCAGCATC AGTCCATTCT
    TCACCCTCTG AGGCAGAATC GAAAAGAAAC GATGAAGTCA TGGGGAATCC AGGGCATGGG
    GAGTTCACTG AGCCATTCAC AGAAAATGGA TTTGGCCTTA CATCTTCCAA GGTCTCAGTG
    GGACTTTGGC AAAATGATTC CCCGACCTCC AGAGGACACC AGCTTGCCAG CAGCTCTGAG
    GCAGAGAACA GAAGCCCCAT GTCTCCGACT CAGACTGTGT CTAGGTCACT CCCATTGGTC
    ACAGATGGAG AAGGCACTGC ACGCTGGTTC TCGACCGACA GCAAGACATT CACAGATGTG
    ACAGGAAGCT CTACTTTCTA TCCTGATGTT GTGAACGCTT CGGATTTGAC TCAGTTCTCA
    GCCTCTGCCC CACAGTCTAG AGGAAGCGAC ACAGCACTGG GTGATAGGGG CTACTCTGAG
    CCGGCCACCG AGACTTTGTC TTCACCTGCC TCAAAGAACC AGAATTCCTC CTCACCACGT
    GGTGAACGCT CCACCACTGA AGACGGTCCT GAGCTGAGAG TCATTTCCGA GGCACAGGAG
    GGGACTTCCG AGGGGGCGAC TGGAGCCCGT GCTCCCAGCC CGCACACTTC AGCCACTTTC
    ACTGGAAGCG GAGAGCGCAC GCTGAGGTCT CTGACCAACG GGAGCACCAC TCCTGGGGAC
    GTGGGGCACT CTGTGGCGGC GCCCAGAGAA ACAGAGAGTG CCACCCAGCA GGGGAACGTG
    ACCATGACTG ATGACGCCCA CCTGGTCTCG GGTTCACCAG CGGCCTCGCC TGCCCTTGGA
    GTCACTGGAA TTGGCAGTCC CTGGAATCAA GTGAGTGGCA CAGACGTTGA AGAGAGAACT
    TCCAGCGACT ACACAGGCCA CACCTATGTT TCGTCTACTT TCCCCAAAGG AGAATGGGCA
    TTACTGTCCA TCACAGACAA CAGCTCATCC TCGGACGTTA GGGAGAGTTC GACCTCTTCT
    ATTAAGATCT CAAACTCTTC CTATTCAGAC TACCCTTCCT CTTCTCAGGC TCAGACTGAA
    AGAAGTAATG TGTCATCCTA CGAAGGGGAA TATGCTCAGC CTTCTACTCA CTCCCTGCTT
    CTGCGCACAG CCAACCTTCC GTCCTATACA CCCACCGTTA ATATGTCAGA CCCTTTGGTG
    CTTCTGGACA GTGATACTGG CTCGCTTGGT GACTCATCTT CTTCTTCATC AGGGTCCCCT
    TTGCCTCTGC CCTCAGTGTC ACAATCCCAC CAGTTGTTCT CATCCACCTT ACCATCAACC
    AGGGCCTCTG CTCACCCACT GCAGTCCACT CCTGGTGCGC CCATACCTTT GTCTTCCTCA
    CCACCACCTG TACCAATATC TCTGACAGCA TCTACCCCTG CCTCGAAGTC CATCTTCCAA
    ACAACCCTAC CGCCTTCATC TTCTACACTC GTCCTGCCCA GGGCAAGGGA CACTCCTGTG
    ACTTCAGTTT GGATGTCAAC AATGACATCT GTAACAATCC TCCCCAGTAG TCGAACAGCA
    GATCCTAAGA ACCAGAGCAA CTCACACCAT GAGCAAATCA TTACGGAATC AGAGCTACCA
    AGCCTGGAGT CTCTGCCCAC AGAGGCCACC GAAGCTGTAA CAATGAGGTC TACTTCAGGG
    ATCTCTATGT CCCCAGCCTC AACAGAGTCC TCTACTGAGC AGACTCTGCC AGCCACAAGC
    ACCAGCGTAG CCCAAACGTC TCCAGCTTTG ACAACCACCT ATCTCGAAAC CTCCTCTCCC
    CCTGTGACCA CTCCCAGCCC CGCATCAAGC ACAGCAGCTC TGACGGCTGG CCCCACAGTA
    CAGACTACAA CCAGAAAACA GCTCTTGACA ACCAGTCCTG AAATTCCAGC ACCTCCGATC
    TCAACAGAAG GTTCTGTCAC CACAGAAAGG AACCAAGTCC GTATAGATGC TACCATCCGA
    TTGGTCCCCC TGACCAGTAC ACCCACTTCG GCAGAAGAAC TGACCACGGG GGTTGGCATT
    ACAGAAGAGG ACACCCCAAC TTCACATTTC CTCAGAACAT CTCCTTCTCC CCAAACCACA
    GATGTCTCTA CAGCTAAAAT GTTGCCTCCT AAATCTACTG CCTCCACTGC TCGGAGCAGC
    ACGCAGTCAC CAACAGCATT GTCCTCTCCA GCCTCAGCTA ACAGCTGTGC CACTAACCCT
    TGTCTTCATG ACGGGAAGTG CGTTGTGGAC CCCGCCAGCC GAGGGTATCA ATGCGTGTGC
    TCACCTTCCT GGCAAGGAGA TGACTGCAGT GTAGATGTGA ATGAATGCCT CTTGAACCCC
    TGCCCACCCC TGGCCACGTG CAACAATACT CAGGGATCCT TCACCTGCAA ATGCCCGGTT
    GGGTACCAGT TGGAAAAAGA AATATGCAAT TTGGTTAGAA CCTTCGTGAC AGATTTTAAA
    TTAAAGAAAA CATTTCTTAA TACCACAGTG GAAAAGTATT CAGACCTACG TGAAGCTGAA
    AAGGAGATCA CCAGAACATT AAATCTGTGT TTCTCCACAT TACCTGGTTA TACCCGATCC
    ACAGTTCACG CTTCTAGGGA GTCCAGCGCG GTGGCCATGT CACTACAAAC CACCTTCTCC
    CTGGCCTCCA ACGTGACGCT GTTCGACCTG GCTGACAGGA TGCAGAAGTG TGTCAACTCC
    TGCAGGTCCT CCGCGGAGGT CTGTCAGCTC TTGGGATCTC AGAAGAGGCT CTTTAGAGCG
    GGCAGCTTGT GCAAGCGGAA GACTCCGGAA TGTGACAAAG AGACCTCCAT CTGCACTGAT
    TTGGACGGCG TGGCGCTGTG TCAGTGCAAG TCGGGGTACT TTCAGTTCAA CAAGATGGAT
    CACTCCTGCC GAGCATGTGA AGATGGATAC AGGCTTGAAA ATGAAACCTG TATGAGTTGC
    CCATTCGGCC TTGGTGGTCT CAACTGTGGA AACCCCTATC AGCTCATCAC CGTGGTGATT
    GCAGCAGCCG GAGGCGGGCT CCTGCTCATC CTGGGCATTG CACTGATTGT CACCTGTTGC
    AGAAAGAATA AAAATGACAT CAGCAAACTC ATCTTCAAAA GTGGGGATTT CCAAATGTCC
    CCATATGCTG AGTACCCCAA GAACCCTCGC TCACAAGAGT GGGGCCGAGA AGCCATTGAG
    ATGCATGAGA ATGGAAGCAC CAAAAACCTC CTCCAGATGA CAGATGTGTA TTACTCGCCC
    ACAAGTGTAA GGAATCCTGA ACTTGAACGA AATGGACTCT ACCCGGCCTA CACTGGATTG
    CCGGGGTCAC GGCATTCTTG CATCTTCCCT GGACAGTATA ACCCATCTTT CATCAGTGAT
    GAGAGCAGGA GAAGAGACTA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.