LOC101338727 cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101338727
Entrez Gene ID	101338727
Full Name	diacylglycerol kinase iota
Synonyms	DGKI
Gene Type	protein-coding
Organism	Tursiops truncatus(common bottlenose dolphin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019950114.1	XP_019805673.1	diacylglycerol kinase iota

Pathways from BioSystems
Gene Ontology

The following LOC101338727 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101338727 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTu29980	XM_019950114.1 Latest version!	Tursiops truncatus diacylglycerol kinase iota (LOC101338727), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101338727 ( XM_019950114.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019805673.1 Tursiops truncatus diacylglycerol kinase iota (LOC101338727), mRNA.

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CloneID	OTu29980
Clone ID Related Accession (Same CDS sequence)	XM_019950114.1
Accession Version	XM_019950114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	diacylglycerol kinase iota
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017842131.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCACC AGATTGAGGA ACCCTGCTCC CTGGGGGCTC ACGCTGCTGT TATTGTCCCG 
CCCACCTGGA TCATTAAGGT GAAGAAACCT CAGAACTCGC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GAACTGAAGT AAGTGAAAAC 
AAAGGTCGTC CTTTTGTGAT AAAACCTATC TCTTCTCCTC TCATGAAACC TTTGCTTGTG 
TTTGTGAACC CCAAGAGTGG AGGCAACCAG GGAACCAAGG TCATGCAGAT GTTCATGTGG 
TACCTGAACC CACGGCAAGT CTTTGATCTT TCTCAGGAAG GGCCAAAAGA CGCGCTTGAA 
TTATATAGGA AAGTACCAAA TCTGCGGATT CTGGCCTGTG GTGGGGATGG AACGGTGGGC 
TGGATCCTTT CCATTCTGGA TGAACTGCAG CTGAGCCCTC AACCTCCGGT TGGGGTCCTT 
CCTCTGGGGA CCGGGAACGA CCTGGCTCGA ACTCTCAACT GGGGAGGGGG CTACACTGAT 
GAACCTGTTT CTAAGATCCT TTGCCAAGTA GAAGATGGGA CGATTGTACA GCTAGATCGC 
TGGAACCTCC ATGTGGAAAG AAACCCAGAC TTGCCTCCAG AAGAACTCGA AGATGGCGTG 
TGTAAGCTTC CTCTGAATGT TTTCAATAAT TACTTCAGCC TCGGATTTGA TGCCCACGTC 
ACACTGGAGT TCCATGAATC CAGAGAAGCA AATCCGGAGA AATTCAACAG TCGTTTTCGA 
AATAAAATGT TCTATGCAGG GGCAGCTTTT TCTGATTTCC TCCAGAGAAG CTCTAGAGAT 
TTATCCAAGC ATGTTAAAGT TGTCTGTGAT GGAACAGATC TCACCCCAAA GATTCAGGAA 
CTGAAGTTCC AGTGTATAGT ATTTTTAAAT ATACCCAGAT ATTGTGCTGG CACAATGCCT 
TGGGGGAACC CAGGAGATCA CCATGACTTT GAACCTCAGC GTCATGACGA TGGTTATATT 
GAAGTCATTG GATTCACCAT GGCCTCTCTG GCTGCCCTGC AAGTCGGGGG CCACGGAGAA 
AGGCTGCACC AGTGTCGGGA GGTCATGCTG CTAACTTACA AATCCATCCC CATGCAAGTG 
GATGGGGAGC CCTGTAGGTT GGCCCCGGCT ATGATTCGGA TCTCGTTGAG GAATCAGGCC 
AACATGGTAC AGAAGAGCAA GAGAAGGACG TCCATGCCTT TACTCAATGA TCCCCAGTCT 
GTCCCAGACC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AAGAGAAGCT CCGGGAAGCT TCCATCCCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGATC GCCTGCAGGA GGACCTGCAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ACGAAACCTC CTTCCCCAGG 
GCGCTCTCAG CTCAGAGACT CTCCCCACGG TGGTGCTTTC TAGATGCTAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CACTTGCACT TTGTGATGGA GATTTCTCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGTTGTCAC AGCAGGCAGG GACACCCCCA 
GGAATGCCTG ATCTGGTGGT GGAGCAAGCC TCCGGAATGA CAGACTGGTG GAATCCTGCC 
CTCCGGAAAC GCATGCTGAG TGACAGTGGG CTGGGAATGA TAACTCCGTA TTACGAAGAA 
GACTCAGATC TGAAAGATTT CAGCCACTCC CGAGTGCTAC AGTCACCAGT CTCTTCAGAA 
GATCACGCAA TTTTGCAGGC AGTAATAGCT GGTGATCTTA TGAAGCTAAT AGAAAGCTAT 
AAAAATGGAG GCAGCCTGCT AATTCAGGGA CCAGACCACT GTTCACTCCT TCACTACGCA 
GCTAAAACCG GCAACGGGGA GATTGTGAAA TATATCCTTG ACCATGGACC TTCTGAGTTA 
TTGGATATGG CAGACAGCGA AACGGGTGAG ACCGCACTGC ACAAGGCAGC TTGCCAGCGG 
AACCGGGCTG TGTGCCAGCT TCTGGTGGAC GCAGGAGCAT CTCTGAGAAA GACGGACTCC 
AAGGGTAAGA CACCTCAAGA GAGAGCACAG CAGGCCGGGG ACCCGGATTT GGCAGCCTAC 
CTAGAAAGCC GCCAGAACTA TAAGATCATT GGCCATGAGG ACCTGGAAAC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019805673.1
CDS	15..2294
Translation	MLHQIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEVSENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVMQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGMTDWWNPALRKRMLSDSGLGMITPYYEEDSDLKDFSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_019950114.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus diacylglycerol kinase iota (LOC101338727), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019950114.1

ORF Insert Sequence:

ATGCTGCACC AGATTGAGGA ACCCTGCTCC CTGGGGGCTC ACGCTGCTGT TATTGTCCCG 
CCCACCTGGA TCATTAAGGT GAAGAAACCT CAGAACTCGC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GAACTGAAGT AAGTGAAAAC 
AAAGGTCGTC CTTTTGTGAT AAAACCTATC TCTTCTCCTC TCATGAAACC TTTGCTTGTG 
TTTGTGAACC CCAAGAGTGG AGGCAACCAG GGAACCAAGG TCATGCAGAT GTTCATGTGG 
TACCTGAACC CACGGCAAGT CTTTGATCTT TCTCAGGAAG GGCCAAAAGA CGCGCTTGAA 
TTATATAGGA AAGTACCAAA TCTGCGGATT CTGGCCTGTG GTGGGGATGG AACGGTGGGC 
TGGATCCTTT CCATTCTGGA TGAACTGCAG CTGAGCCCTC AACCTCCGGT TGGGGTCCTT 
CCTCTGGGGA CCGGGAACGA CCTGGCTCGA ACTCTCAACT GGGGAGGGGG CTACACTGAT 
GAACCTGTTT CTAAGATCCT TTGCCAAGTA GAAGATGGGA CGATTGTACA GCTAGATCGC 
TGGAACCTCC ATGTGGAAAG AAACCCAGAC TTGCCTCCAG AAGAACTCGA AGATGGCGTG 
TGTAAGCTTC CTCTGAATGT TTTCAATAAT TACTTCAGCC TCGGATTTGA TGCCCACGTC 
ACACTGGAGT TCCATGAATC CAGAGAAGCA AATCCGGAGA AATTCAACAG TCGTTTTCGA 
AATAAAATGT TCTATGCAGG GGCAGCTTTT TCTGATTTCC TCCAGAGAAG CTCTAGAGAT 
TTATCCAAGC ATGTTAAAGT TGTCTGTGAT GGAACAGATC TCACCCCAAA GATTCAGGAA 
CTGAAGTTCC AGTGTATAGT ATTTTTAAAT ATACCCAGAT ATTGTGCTGG CACAATGCCT 
TGGGGGAACC CAGGAGATCA CCATGACTTT GAACCTCAGC GTCATGACGA TGGTTATATT 
GAAGTCATTG GATTCACCAT GGCCTCTCTG GCTGCCCTGC AAGTCGGGGG CCACGGAGAA 
AGGCTGCACC AGTGTCGGGA GGTCATGCTG CTAACTTACA AATCCATCCC CATGCAAGTG 
GATGGGGAGC CCTGTAGGTT GGCCCCGGCT ATGATTCGGA TCTCGTTGAG GAATCAGGCC 
AACATGGTAC AGAAGAGCAA GAGAAGGACG TCCATGCCTT TACTCAATGA TCCCCAGTCT 
GTCCCAGACC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AAGAGAAGCT CCGGGAAGCT TCCATCCCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGATC GCCTGCAGGA GGACCTGCAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ACGAAACCTC CTTCCCCAGG 
GCGCTCTCAG CTCAGAGACT CTCCCCACGG TGGTGCTTTC TAGATGCTAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CACTTGCACT TTGTGATGGA GATTTCTCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGTTGTCAC AGCAGGCAGG GACACCCCCA 
GGAATGCCTG ATCTGGTGGT GGAGCAAGCC TCCGGAATGA CAGACTGGTG GAATCCTGCC 
CTCCGGAAAC GCATGCTGAG TGACAGTGGG CTGGGAATGA TAACTCCGTA TTACGAAGAA 
GACTCAGATC TGAAAGATTT CAGCCACTCC CGAGTGCTAC AGTCACCAGT CTCTTCAGAA 
GATCACGCAA TTTTGCAGGC AGTAATAGCT GGTGATCTTA TGAAGCTAAT AGAAAGCTAT 
AAAAATGGAG GCAGCCTGCT AATTCAGGGA CCAGACCACT GTTCACTCCT TCACTACGCA 
GCTAAAACCG GCAACGGGGA GATTGTGAAA TATATCCTTG ACCATGGACC TTCTGAGTTA 
TTGGATATGG CAGACAGCGA AACGGGTGAG ACCGCACTGC ACAAGGCAGC TTGCCAGCGG 
AACCGGGCTG TGTGCCAGCT TCTGGTGGAC GCAGGAGCAT CTCTGAGAAA GACGGACTCC 
AAGGGTAAGA CACCTCAAGA GAGAGCACAG CAGGCCGGGG ACCCGGATTT GGCAGCCTAC 
CTAGAAAGCC GCCAGAACTA TAAGATCATT GGCCATGAGG ACCTGGAAAC TGCTGTTTGA