TTC3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TTC3
Entrez Gene ID	478414
Full Name	tetratricopeptide repeat domain 3
General protein information	Preferred Names tetratricopeptide repeat domain 3 Names E3 ubiquitin-protein ligase TTC3
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005638906.3	XP_005638963.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_005638906.2	XP_005638963.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_005638905.2	XP_005638962.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_005638907.2	XP_005638964.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_005638907.3	XP_005638964.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_014109729.1	XP_013965204.1	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_005638905.3	XP_005638962.1	E3 ubiquitin-protein ligase TTC3 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ttc3	NP_001101785.1
Bos taurus (cattle)	TTC3	NP_001103237.1
Xenopus tropicalis (tropical clawed frog)	ttc3	XP_002941578.2
	TTC3	XP_001098049.2
Gallus gallus (chicken)	TTC3	XP_416727.4
Danio rerio (zebrafish)	LOC100329450	XP_005172636.1
Pan troglodytes (chimpanzee)	TTC3	XP_001169932.1
Canis lupus familiaris (dog)	TTC3	XP_849567.2
Mus musculus (house mouse)	Ttc3	NP_033467.2
Homo sapiens (human)	TTC3	NP_001001894.1

Gene Ontology

The following TTC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe29425	XM_005638906.3 Latest version!	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29427	XM_005638907.3 Latest version!	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29424	XM_014109729.1 Latest version!	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe29424	XM_005638905.3 Latest version!	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe29425	XM_005638906.2	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29427	XM_005638907.2	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29424	XM_005638905.2	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTC3 ( XM_005638906.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638963.1 Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

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CloneID	OCe29425
Clone ID Related Accession (Same CDS sequence)	XM_005638906.3 , XM_005638906.2
Accession Version	XM_005638906.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATT TTGCCCTGGG AGATTTCACT ATAGCAGATG ATGCTTTGTC AGAAGATTGC 
CCTTATGTGG ACGGTTGTGT CTTTGCTCCT GAATTTATGT CCAATGATTA TGTTCGTGTG 
ACTCAACTTT ACTGCGATGG GGTGGGTATG CAGTATAAAG ATTTTACCCA AAGTGAGAGA 
AACTTAGAAT TTGACATCTG TAATATGTGG TGTAGTAAAC CAGTTTCTGT CCTGCAAGAT 
TATTGTGATG CCATTAAAAT ATACATCTTC TGGCCACTTC TGTTTCAACG TCAGCATAGT 
TCTATAATAT CACGGTTACA CCCCTGTGTG GAGGCCACCA ACTCTCATGC TTCTGAGATA 
AGTTTGAAGA AATTGCAACA TCTAGAATTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTCGTGAATG ATTCATTCTT TATTGGGGGC TTATTGAGGA TTGGTTATAA AATAGAAAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT ATACAGGCGA TGTAACAATT 
CTACCTAAAT TAGGAGCAGT TGACAATTGT TGGCCTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AGTTGTAACA GAAAACTGCA ATTTGCTTGA AGAATTTAAA 
ACTCAAAATT GTGCGGAATG TATAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCAA AAGAAAGATT TGATATAGCT ATTATCTATT ACACCAGAGC CATTGAATAT 
AGACCTGAAA ATCACCTTCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG CACTGGACAG 
TTTAGAAATG CACTTGGTGA TGGAAAGAGA GCCACTATCC TGAAGAGCAA TTGGACGAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCGAAC ACCAACTAGA 
AATCCAATTA AAGCCTTTTA TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAGATT CCAGAAAAAC TAACCGCGAA 
ATGGCAAACG GTGGTAATCA GAATCTAAAG GTGGTAGATG AAGCTTTGAA GGTAGATGAT 
TGTGACTGTC ATCCTGAATT TCTACCACCA TCAGGTCAGA CTCCAAAATA CAAAGGAAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGTTCTA GTTCACAGGG GAGTTTACCA 
GTAGATTTGA AGAACATTTT GGAAAAACAA TTCTCTAAAT CTTCCAGAGC TGCACATCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGTTA CACGGCTTTG 
TTGGAGCAGC GTTGCCGCAG TGCTGCACAG GCCTTCACAG AATTGCTCAA TGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAACCTGGCC ATGATTAACT ATGTCTTAGT AGTCTACGGA 
CTTGCTATTT CTCTCCTTGG AATAGGACAG CCTGAGGAAT TATCTGAAGC TGAAAACCAG 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGTTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAGAACAGA TTTCTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATTTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGATT 
ATTGAAGAGA CTCAGCCGGG GAAAATAAAG ATGCTGTTAG AGAAGTTTAT TGAAGAATGC 
AGGTTCCCTC CAGTGCCAGA TGCCATTTGT TGCTATCAGA AGTGCCGTGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGAATCAG CTGCTGCCAG 
TACTGTAAAA TAGAATTTCA CATGAACTGT TGGAAAAAGT TGAAAACAAC AACTTTTAAT 
GATAAAATTG ACAAGGATTT TCTACAAGGA ATTTGTCTTA CCCCTGACTG TGAAGGAATC 
ATTTCTAAGA TTATCATTTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATAAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGCTAAGAC TGAAAGAAGA CAAAAAATTG AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA 
AAATTAGCAC AAGAAAGAAT GGAAGAGGAC CTAAGGGAAA GTAATCCACC CAAAACTGAA 
GAGCAGAAAG AAACTGTAGA CAGTGTTCAG AGTTGCCAGT TCCTTGACGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATA AAATCTGGTA TTCTGAACAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA AGCACAGAAG ACTACACAAC CTGTTTTTCT 
AGCAGAAATT TTCTAAATGA AGCGGTGGAC TACGTCATTC GTCACTTGAT TCAAGAAAAG 
AATAGAGTAA AGACTAGGAT ATTTCTGCAT GTTTTGAGCG AACTTAAAGA AGTGGAGCCA 
AAATTAGCCG CTTGGATCCA GAAACTTAAC AGCTTTGGTT TAGATGCCAC AGGACCTTTT 
TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA ACTAGCCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTGT 
GAGCCAACAT CAGTGAAGGA AGCACGGTGT TTAATATGGC TGCTAGAAGA GCATAGAGAC 
AAGTTCCCAG CGCTACACAA TGCTCTAGAT GAGTTCTTTG ACATAATGGA TAGCCGCTGT 
ACTGTATTAA GGAAACAAGA CAGTGGCGAT GTGCCGTTTA ATTCCGCCAA GATAAAAAAT 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TGGTGGGGTC TGGGACACCT 
TCTGTAACAC CAAATAATGA GACTATCACT TCGGGTGAAG AACATAAACG CAATTCAAAT 
TCTGCAGGGC CATTTGTGGT ACCTGACCAC CTTCGGCAAG ATGTAGAAGA ATTTGAAGCT 
ATCTATGAGC AGCACAGTAG TGAATATGTT GTCCGTAATA AAAAGCTGTG GGACATTAAC 
CCAAAACAAA AATGTTCAAC TCTGTATGAT TATTTCTCTC AGTTGTTGGA GGAGCATGGT 
CCCTTGGACA TGAGTAATAA AATGTTCTCT GAAGAATATG AATTCTTCCC AGAAGAAACT 
AGACAGATAC TAGAAAAAGC TGGAGGTTTG AAATCTTTCC TCCTGGGATG TCCTCGTTTT 
GTTGTGATTG ACAACTGTAT TGCTTTGAAG AAAGTTGCTT TACGGCTCAA GAAAAAGAGA 
AAGAAGAAAA ACATTAAGAC AAAAGTGGAA GAAATTTCAA AAACAGGGGA GTATTTACGA 
GTTAAACTAC CACTGAATCC AACTGCTAGG GAATTTAAAC CAGATGTAAA GTCCAAACCA 
ATGTCTGATT TATCTTCAGC AACAGCTTCT GAGAATGTGA AGCCCAAACC TGTAGCTGCT 
AATTCTCCCA AATCAACCTG TGAAGTTATG AAGCCAAGAC CAGTGTCTGA TAATTCTTCT 
AGATCAGTTT CTGAAGATGA GAAGCCCAAA GAAGTCTCTT CTGATTCTGC CCAACCTGTA 
TCTGAGGATG CAAGTCACAA GCGAGTATCC TCTAATTCTC CTAAACCAGC TTCTGAGGAT 
GTGAAACCAG CCTACTGGAC TCAGCCCCAT TTGGTCACAG GATACTGTAC ATACCTCCCC 
TTTCGGGGAT TTGACATTAC CCAGACACCA CCAACATACA TAAATGTGTT ACCAACTTTG 
CCCCAGTACA CTAACATTTA CACACCTCTG CCCAATGTTT CTTCTGAATA TCAGCTGCAA 
AGATCAGTGC CAGTGGTGCC GTCTTTTATA CCCAGTGAAA GACTAGATGA AAGTGCTACT 
GTGTACTTTG AGGGTCATCA TTTGAATGCT GAGAATGCCC CTGGGAACCA GATTGTCCCT 
AAAACACAGA TCCTTCAGGA CTCTATGGAA GTGTCAGTAA AGACAGAGTG CAGCACAGAT 
GGTGCTGATA CAGCCCTTAG TGAGTCTAAC AGATATGATG GTCACTGTGG AAATTCTGCC 
AACAAGTGGG AAGTAAATCC AGAAAAAAAC AATGAAGTAA CAAATACTCC ACATACACAA 
ATGGTTGCCA TACAGGTGTC TTGGAATATA ACACATCAAG AAGTCAATAC TGAGCCATAT 
GATCCTTTTG AGACACAACA GGGAGATATT TCACAGATTG AGAAGGAGTT CCAGGTTTTA 
CAAGAGCAGC TTAACGAGGC ATGTGAAAAT TATGAGCAGA GAAAACTCAA GGGCTCAGAA 
GAGACCAGGG ATCTGGAAGA AAAATTGAAA AGGAACTTAG AAGAAAACAA GATCTCAAAG 
ACAGAATTAG ATTGGTTCCT TGAAGACTTG GAAAAGGAAA TCAAGAAATG GCAACAGGAG 
AAAAAAGAAA TCCAAGAAAG TCTGAAAGCG CTAACAAAGA AAATGAGAAA GGTTTTAAAT 
GCCAACGAGA TATATGCCCA GAAAAATGAT GGAAAGGAAA AGGAACATGA ATTACTTCTG 
GATCAGTCCC TTGAAATCAG TAATACGTTT ACAAATGAGA AAATGAAAGT AGAAGAATGT 
ATAAAGAAAG GGAAGGAGAA TTATGAAGAG AGTCTTCAAA GAGCTGTGGC TGCAGAGGTA 
TCTGTGCTTG AAAACTGGAA GGACATTGAA GTGTACAAAT TACAGATCAT GGAATCACAA 
GCAGAAGGGT ATCTTAAGAA CCTGAGGCTA CTGAGCAGTG ATTCTGCAAC TTACCCTGAT 
AAGGAATCTG ATATACATTC ATGGGAATCA TTTCTTTCTA AGGTTAAAAA AGAAATTGAA 
AAAGCAAAGT CTCAGTATGA AGAACAAATT CAAGCAATTA AAAATGGCTC TCGACTCAGT 
GAACTTTCTA AAGTGCAAGT TCCTGAGCTT TCATTTCCTG TCTGTAGCAC AATTCTTCCT 
ACATCACTTC CTGAGTCTTC AGGCCATGAA GATCACAGAG CCTCCATTTC TACAAGTCAT 
GAGGCTGGAA ACCAAACAGC AGTTCCTAAG GACTCAAGTC TGGTCTCTGC CAGTGACGGC 
CCAGTGGGGG CTTCCTCTCC ACCATTGACA GGATCGCTCT CCTGTCAGCC CGAAGTCACC 
CAGCTGCCAG AGTGTAAGAG CACAGGCCAG GAAACTCCGC CAAAACAAAG CCTTGTGGCT 
GATCAGAAAC TACCTGTTCT TCCAGGACGT GCTACCCGCT CAAGCCAGTC TCCAAAAAAG 
CCGTTCAACA GTATTATTGA GCACCTGTCA GTGGTATTCC CATGTTACAA CAGCACTGAG 
CTTGCTGGTT TTATTAAAAA AGTGCGAAAC AAGAACAAAA ACTCGCTCTC AGGATTGAGT 
ATTGATGAAA TTGTCCAAAG AGTGACAGAA CACATTCTAG ATGAACAGAA AAAGAAAAAG 
CCAAATTCAA ATTCAGCAAA GGACAAGAGG TTGTCTGAGC CCAGCTCCAC TGCTTCTGTG 
AACAGGGCCT CCCAGGGTCC ACCCTCCGTG ACTGCTGGAC CATCGCCCAA AACCAAGGGA 
CAAAAGACAG AAGATGCCCC TGCATCGGGT GCAGCTTCCT GTGAACTGTG CCATGAGATA 
TTCAAATCCA AAAACGTGCG TGTACTAAAA TGTGGGCACA AGTTTCACAA AGGGTGCTTT 
AAGCAGTGGC TGAAGGGGCA GAGCACTTGC CCGGCCTGCC TCGATCAGGA TCTCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638963.1
CDS	1607..7606
Translation	MDDFALGDFTIADDALSEDCPYVDGCVFAPEFMSNDYVRVTQLYCDGVGMQYKDFTQSERNLEFDICNMWCSKPVSVLQDYCDAIKIYIFWPLLFQRQHSSIISRLHPCVEATNSHASEISLKKLQHLELMEDIVDLAKKVVNDSFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILPKLGAVDNCWPMLSIFFTEYKYHITKVVTENCNLLEEFKTQNCAECIEQGELMKMKGNEEFSKERFDIAIIYYTRAIEYRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKSNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPTRNPIKAFYESRAYIPSLSAPAFSTSLNFVETEKDSRKTNREMANGGNQNLKVVDEALKVDDCDCHPEFLPPSGQTPKYKGKQKSRNNELEKFSSSSQGSLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFIEECRFPPVPDAICCYQKCRGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKIQKKEAKKLAQERMEEDLRESNPPKTEEQKETVDSVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLSTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRIFLHVLSELKEVEPKLAAWIQKLNSFGLDATGPFFSRYGASLKELDFSIMTFLWNEKYGHKLASIEGKQLDYFCEPTSVKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSAKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETITSGEEHKRNSNSAGPFVVPDHLRQDVEEFEAIYEQHSSEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKSFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPMSDLSSATASENVKPKPVAANSPKSTCEVMKPRPVSDNSSRSVSEDEKPKEVSSDSAQPVSEDASHKRVSSNSPKPASEDVKPAYWTQPHLVTGYCTYLPFRGFDITQTPPTYINVLPTLPQYTNIYTPLPNVSSEYQLQRSVPVVPSFIPSERLDESATVYFEGHHLNAENAPGNQIVPKTQILQDSMEVSVKTECSTDGADTALSESNRYDGHCGNSANKWEVNPEKNNEVTNTPHTQMVAIQVSWNITHQEVNTEPYDPFETQQGDISQIEKEFQVLQEQLNEACENYEQRKLKGSEETRDLEEKLKRNLEENKISKTELDWFLEDLEKEIKKWQQEKKEIQESLKALTKKMRKVLNANEIYAQKNDGKEKEHELLLDQSLEISNTFTNEKMKVEECIKKGKENYEESLQRAVAAEVSVLENWKDIEVYKLQIMESQAEGYLKNLRLLSSDSATYPDKESDIHSWESFLSKVKKEIEKAKSQYEEQIQAIKNGSRLSELSKVQVPELSFPVCSTILPTSLPESSGHEDHRASISTSHEAGNQTAVPKDSSLVSASDGPVGASSPPLTGSLSCQPEVTQLPECKSTGQETPPKQSLVADQKLPVLPGRATRSSQSPKKPFNSIIEHLSVVFPCYNSTELAGFIKKVRNKNKNSLSGLSIDEIVQRVTEHILDEQKKKKPNSNSAKDKRLSEPSSTASVNRASQGPPSVTAGPSPKTKGQKTEDAPASGAASCELCHEIFKSKNVRVLKCGHKFHKGCFKQWLKGQSTCPACLDQDLL

Target ORF information:

RefSeq Version	XM_005638906.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638906.3

ORF Insert Sequence:

ATGGATGATT TTGCCCTGGG AGATTTCACT ATAGCAGATG ATGCTTTGTC AGAAGATTGC 
CCTTATGTGG ACGGTTGTGT CTTTGCTCCT GAATTTATGT CCAATGATTA TGTTCGTGTG 
ACTCAACTTT ACTGCGATGG GGTGGGTATG CAGTATAAAG ATTTTACCCA AAGTGAGAGA 
AACTTAGAAT TTGACATCTG TAATATGTGG TGTAGTAAAC CAGTTTCTGT CCTGCAAGAT 
TATTGTGATG CCATTAAAAT ATACATCTTC TGGCCACTTC TGTTTCAACG TCAGCATAGT 
TCTATAATAT CACGGTTACA CCCCTGTGTG GAGGCCACCA ACTCTCATGC TTCTGAGATA 
AGTTTGAAGA AATTGCAACA TCTAGAATTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTCGTGAATG ATTCATTCTT TATTGGGGGC TTATTGAGGA TTGGTTATAA AATAGAAAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT ATACAGGCGA TGTAACAATT 
CTACCTAAAT TAGGAGCAGT TGACAATTGT TGGCCTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AGTTGTAACA GAAAACTGCA ATTTGCTTGA AGAATTTAAA 
ACTCAAAATT GTGCGGAATG TATAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCAA AAGAAAGATT TGATATAGCT ATTATCTATT ACACCAGAGC CATTGAATAT 
AGACCTGAAA ATCACCTTCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG CACTGGACAG 
TTTAGAAATG CACTTGGTGA TGGAAAGAGA GCCACTATCC TGAAGAGCAA TTGGACGAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCGAAC ACCAACTAGA 
AATCCAATTA AAGCCTTTTA TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAGATT CCAGAAAAAC TAACCGCGAA 
ATGGCAAACG GTGGTAATCA GAATCTAAAG GTGGTAGATG AAGCTTTGAA GGTAGATGAT 
TGTGACTGTC ATCCTGAATT TCTACCACCA TCAGGTCAGA CTCCAAAATA CAAAGGAAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGTTCTA GTTCACAGGG GAGTTTACCA 
GTAGATTTGA AGAACATTTT GGAAAAACAA TTCTCTAAAT CTTCCAGAGC TGCACATCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGTTA CACGGCTTTG 
TTGGAGCAGC GTTGCCGCAG TGCTGCACAG GCCTTCACAG AATTGCTCAA TGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAACCTGGCC ATGATTAACT ATGTCTTAGT AGTCTACGGA 
CTTGCTATTT CTCTCCTTGG AATAGGACAG CCTGAGGAAT TATCTGAAGC TGAAAACCAG 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGTTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAGAACAGA TTTCTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATTTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGATT 
ATTGAAGAGA CTCAGCCGGG GAAAATAAAG ATGCTGTTAG AGAAGTTTAT TGAAGAATGC 
AGGTTCCCTC CAGTGCCAGA TGCCATTTGT TGCTATCAGA AGTGCCGTGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGAATCAG CTGCTGCCAG 
TACTGTAAAA TAGAATTTCA CATGAACTGT TGGAAAAAGT TGAAAACAAC AACTTTTAAT 
GATAAAATTG ACAAGGATTT TCTACAAGGA ATTTGTCTTA CCCCTGACTG TGAAGGAATC 
ATTTCTAAGA TTATCATTTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATAAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGCTAAGAC TGAAAGAAGA CAAAAAATTG AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA 
AAATTAGCAC AAGAAAGAAT GGAAGAGGAC CTAAGGGAAA GTAATCCACC CAAAACTGAA 
GAGCAGAAAG AAACTGTAGA CAGTGTTCAG AGTTGCCAGT TCCTTGACGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATA AAATCTGGTA TTCTGAACAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA AGCACAGAAG ACTACACAAC CTGTTTTTCT 
AGCAGAAATT TTCTAAATGA AGCGGTGGAC TACGTCATTC GTCACTTGAT TCAAGAAAAG 
AATAGAGTAA AGACTAGGAT ATTTCTGCAT GTTTTGAGCG AACTTAAAGA AGTGGAGCCA 
AAATTAGCCG CTTGGATCCA GAAACTTAAC AGCTTTGGTT TAGATGCCAC AGGACCTTTT 
TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA ACTAGCCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTGT 
GAGCCAACAT CAGTGAAGGA AGCACGGTGT TTAATATGGC TGCTAGAAGA GCATAGAGAC 
AAGTTCCCAG CGCTACACAA TGCTCTAGAT GAGTTCTTTG ACATAATGGA TAGCCGCTGT 
ACTGTATTAA GGAAACAAGA CAGTGGCGAT GTGCCGTTTA ATTCCGCCAA GATAAAAAAT 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TGGTGGGGTC TGGGACACCT 
TCTGTAACAC CAAATAATGA GACTATCACT TCGGGTGAAG AACATAAACG CAATTCAAAT 
TCTGCAGGGC CATTTGTGGT ACCTGACCAC CTTCGGCAAG ATGTAGAAGA ATTTGAAGCT 
ATCTATGAGC AGCACAGTAG TGAATATGTT GTCCGTAATA AAAAGCTGTG GGACATTAAC 
CCAAAACAAA AATGTTCAAC TCTGTATGAT TATTTCTCTC AGTTGTTGGA GGAGCATGGT 
CCCTTGGACA TGAGTAATAA AATGTTCTCT GAAGAATATG AATTCTTCCC AGAAGAAACT 
AGACAGATAC TAGAAAAAGC TGGAGGTTTG AAATCTTTCC TCCTGGGATG TCCTCGTTTT 
GTTGTGATTG ACAACTGTAT TGCTTTGAAG AAAGTTGCTT TACGGCTCAA GAAAAAGAGA 
AAGAAGAAAA ACATTAAGAC AAAAGTGGAA GAAATTTCAA AAACAGGGGA GTATTTACGA 
GTTAAACTAC CACTGAATCC AACTGCTAGG GAATTTAAAC CAGATGTAAA GTCCAAACCA 
ATGTCTGATT TATCTTCAGC AACAGCTTCT GAGAATGTGA AGCCCAAACC TGTAGCTGCT 
AATTCTCCCA AATCAACCTG TGAAGTTATG AAGCCAAGAC CAGTGTCTGA TAATTCTTCT 
AGATCAGTTT CTGAAGATGA GAAGCCCAAA GAAGTCTCTT CTGATTCTGC CCAACCTGTA 
TCTGAGGATG CAAGTCACAA GCGAGTATCC TCTAATTCTC CTAAACCAGC TTCTGAGGAT 
GTGAAACCAG CCTACTGGAC TCAGCCCCAT TTGGTCACAG GATACTGTAC ATACCTCCCC 
TTTCGGGGAT TTGACATTAC CCAGACACCA CCAACATACA TAAATGTGTT ACCAACTTTG 
CCCCAGTACA CTAACATTTA CACACCTCTG CCCAATGTTT CTTCTGAATA TCAGCTGCAA 
AGATCAGTGC CAGTGGTGCC GTCTTTTATA CCCAGTGAAA GACTAGATGA AAGTGCTACT 
GTGTACTTTG AGGGTCATCA TTTGAATGCT GAGAATGCCC CTGGGAACCA GATTGTCCCT 
AAAACACAGA TCCTTCAGGA CTCTATGGAA GTGTCAGTAA AGACAGAGTG CAGCACAGAT 
GGTGCTGATA CAGCCCTTAG TGAGTCTAAC AGATATGATG GTCACTGTGG AAATTCTGCC 
AACAAGTGGG AAGTAAATCC AGAAAAAAAC AATGAAGTAA CAAATACTCC ACATACACAA 
ATGGTTGCCA TACAGGTGTC TTGGAATATA ACACATCAAG AAGTCAATAC TGAGCCATAT 
GATCCTTTTG AGACACAACA GGGAGATATT TCACAGATTG AGAAGGAGTT CCAGGTTTTA 
CAAGAGCAGC TTAACGAGGC ATGTGAAAAT TATGAGCAGA GAAAACTCAA GGGCTCAGAA 
GAGACCAGGG ATCTGGAAGA AAAATTGAAA AGGAACTTAG AAGAAAACAA GATCTCAAAG 
ACAGAATTAG ATTGGTTCCT TGAAGACTTG GAAAAGGAAA TCAAGAAATG GCAACAGGAG 
AAAAAAGAAA TCCAAGAAAG TCTGAAAGCG CTAACAAAGA AAATGAGAAA GGTTTTAAAT 
GCCAACGAGA TATATGCCCA GAAAAATGAT GGAAAGGAAA AGGAACATGA ATTACTTCTG 
GATCAGTCCC TTGAAATCAG TAATACGTTT ACAAATGAGA AAATGAAAGT AGAAGAATGT 
ATAAAGAAAG GGAAGGAGAA TTATGAAGAG AGTCTTCAAA GAGCTGTGGC TGCAGAGGTA 
TCTGTGCTTG AAAACTGGAA GGACATTGAA GTGTACAAAT TACAGATCAT GGAATCACAA 
GCAGAAGGGT ATCTTAAGAA CCTGAGGCTA CTGAGCAGTG ATTCTGCAAC TTACCCTGAT 
AAGGAATCTG ATATACATTC ATGGGAATCA TTTCTTTCTA AGGTTAAAAA AGAAATTGAA 
AAAGCAAAGT CTCAGTATGA AGAACAAATT CAAGCAATTA AAAATGGCTC TCGACTCAGT 
GAACTTTCTA AAGTGCAAGT TCCTGAGCTT TCATTTCCTG TCTGTAGCAC AATTCTTCCT 
ACATCACTTC CTGAGTCTTC AGGCCATGAA GATCACAGAG CCTCCATTTC TACAAGTCAT 
GAGGCTGGAA ACCAAACAGC AGTTCCTAAG GACTCAAGTC TGGTCTCTGC CAGTGACGGC 
CCAGTGGGGG CTTCCTCTCC ACCATTGACA GGATCGCTCT CCTGTCAGCC CGAAGTCACC 
CAGCTGCCAG AGTGTAAGAG CACAGGCCAG GAAACTCCGC CAAAACAAAG CCTTGTGGCT 
GATCAGAAAC TACCTGTTCT TCCAGGACGT GCTACCCGCT CAAGCCAGTC TCCAAAAAAG 
CCGTTCAACA GTATTATTGA GCACCTGTCA GTGGTATTCC CATGTTACAA CAGCACTGAG 
CTTGCTGGTT TTATTAAAAA AGTGCGAAAC AAGAACAAAA ACTCGCTCTC AGGATTGAGT 
ATTGATGAAA TTGTCCAAAG AGTGACAGAA CACATTCTAG ATGAACAGAA AAAGAAAAAG 
CCAAATTCAA ATTCAGCAAA GGACAAGAGG TTGTCTGAGC CCAGCTCCAC TGCTTCTGTG 
AACAGGGCCT CCCAGGGTCC ACCCTCCGTG ACTGCTGGAC CATCGCCCAA AACCAAGGGA 
CAAAAGACAG AAGATGCCCC TGCATCGGGT GCAGCTTCCT GTGAACTGTG CCATGAGATA 
TTCAAATCCA AAAACGTGCG TGTACTAAAA TGTGGGCACA AGTTTCACAA AGGGTGCTTT 
AAGCAGTGGC TGAAGGGGCA GAGCACTTGC CCGGCCTGCC TCGATCAGGA TCTCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005638906.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638963.1 Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

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CloneID	OCe29425
Clone ID Related Accession (Same CDS sequence)	XM_005638906.3 , XM_005638906.2
Accession Version	XM_005638906.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638906.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATT TTGCCCTGGG AGATTTCACT ATAGCAGATG ATGCTTTGTC AGAAGATTGC 
CCTTATGTGG ACGGTTGTGT CTTTGCTCCT GAATTTATGT CCAATGATTA TGTTCGTGTG 
ACTCAACTTT ACTGCGATGG GGTGGGTATG CAGTATAAAG ATTTTACCCA AAGTGAGAGA 
AACTTAGAAT TTGACATCTG TAATATGTGG TGTAGTAAAC CAGTTTCTGT CCTGCAAGAT 
TATTGTGATG CCATTAAAAT ATACATCTTC TGGCCACTTC TGTTTCAACG TCAGCATAGT 
TCTATAATAT CACGGTTACA CCCCTGTGTG GAGGCCACCA ACTCTCATGC TTCTGAGATA 
AGTTTGAAGA AATTGCAACA TCTAGAATTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTCGTGAATG ATTCATTCTT TATTGGGGGC TTATTGAGGA TTGGTTATAA AATAGAAAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT ATACAGGCGA TGTAACAATT 
CTACCTAAAT TAGGAGCAGT TGACAATTGT TGGCCTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AGTTGTAACA GAAAACTGCA ATTTGCTTGA AGAATTTAAA 
ACTCAAAATT GTGCGGAATG TATAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCAA AAGAAAGATT TGATATAGCT ATTATCTATT ACACCAGAGC CATTGAATAT 
AGACCTGAAA ATCACCTTCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG CACTGGACAG 
TTTAGAAATG CACTTGGTGA TGGAAAGAGA GCCACTATCC TGAAGAGCAA TTGGACGAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCGAAC ACCAACTAGA 
AATCCAATTA AAGCCTTTTA TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAGATT CCAGAAAAAC TAACCGCGAA 
ATGGCAAACG GTGGTAATCA GAATCTAAAG GTGGTAGATG AAGCTTTGAA GGTAGATGAT 
TGTGACTGTC ATCCTGAATT TCTACCACCA TCAGGTCAGA CTCCAAAATA CAAAGGAAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGTTCTA GTTCACAGGG GAGTTTACCA 
GTAGATTTGA AGAACATTTT GGAAAAACAA TTCTCTAAAT CTTCCAGAGC TGCACATCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGTTA CACGGCTTTG 
TTGGAGCAGC GTTGCCGCAG TGCTGCACAG GCCTTCACAG AATTGCTCAA TGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAACCTGGCC ATGATTAACT ATGTCTTAGT AGTCTACGGA 
CTTGCTATTT CTCTCCTTGG AATAGGACAG CCTGAGGAAT TATCTGAAGC TGAAAACCAG 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGTTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAGAACAGA TTTCTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATTTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGATT 
ATTGAAGAGA CTCAGCCGGG GAAAATAAAG ATGCTGTTAG AGAAGTTTAT TGAAGAATGC 
AGGTTCCCTC CAGTGCCAGA TGCCATTTGT TGCTATCAGA AGTGCCGTGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGAATCAG CTGCTGCCAG 
TACTGTAAAA TAGAATTTCA CATGAACTGT TGGAAAAAGT TGAAAACAAC AACTTTTAAT 
GATAAAATTG ACAAGGATTT TCTACAAGGA ATTTGTCTTA CCCCTGACTG TGAAGGAATC 
ATTTCTAAGA TTATCATTTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATAAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGCTAAGAC TGAAAGAAGA CAAAAAATTG AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA 
AAATTAGCAC AAGAAAGAAT GGAAGAGGAC CTAAGGGAAA GTAATCCACC CAAAACTGAA 
GAGCAGAAAG AAACTGTAGA CAGTGTTCAG AGTTGCCAGT TCCTTGACGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATA AAATCTGGTA TTCTGAACAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA AGCACAGAAG ACTACACAAC CTGTTTTTCT 
AGCAGAAATT TTCTAAATGA AGCGGTGGAC TACGTCATTC GTCACTTGAT TCAAGAAAAG 
AATAGAGTAA AGACTAGGAT ATTTCTGCAT GTTTTGAGCG AACTTAAAGA AGTGGAGCCA 
AAATTAGCCG CTTGGATCCA GAAACTTAAC AGCTTTGGTT TAGATGCCAC AGGACCTTTT 
TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA ACTAGCCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTGT 
GAGCCAACAT CAGTGAAGGA AGCACGGTGT TTAATATGGC TGCTAGAAGA GCATAGAGAC 
AAGTTCCCAG CGCTACACAA TGCTCTAGAT GAGTTCTTTG ACATAATGGA TAGCCGCTGT 
ACTGTATTAA GGAAACAAGA CAGTGGCGAT GTGCCGTTTA ATTCCGCCAA GATAAAAAAT 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TGGTGGGGTC TGGGACACCT 
TCTGTAACAC CAAATAATGA GACTATCACT TCGGGTGAAG AACATAAACG CAATTCAAAT 
TCTGCAGGGC CATTTGTGGT ACCTGACCAC CTTCGGCAAG ATGTAGAAGA ATTTGAAGCT 
ATCTATGAGC AGCACAGTAG TGAATATGTT GTCCGTAATA AAAAGCTGTG GGACATTAAC 
CCAAAACAAA AATGTTCAAC TCTGTATGAT TATTTCTCTC AGTTGTTGGA GGAGCATGGT 
CCCTTGGACA TGAGTAATAA AATGTTCTCT GAAGAATATG AATTCTTCCC AGAAGAAACT 
AGACAGATAC TAGAAAAAGC TGGAGGTTTG AAATCTTTCC TCCTGGGATG TCCTCGTTTT 
GTTGTGATTG ACAACTGTAT TGCTTTGAAG AAAGTTGCTT TACGGCTCAA GAAAAAGAGA 
AAGAAGAAAA ACATTAAGAC AAAAGTGGAA GAAATTTCAA AAACAGGGGA GTATTTACGA 
GTTAAACTAC CACTGAATCC AACTGCTAGG GAATTTAAAC CAGATGTAAA GTCCAAACCA 
ATGTCTGATT TATCTTCAGC AACAGCTTCT GAGAATGTGA AGCCCAAACC TGTAGCTGCT 
AATTCTCCCA AATCAACCTG TGAAGTTATG AAGCCAAGAC CAGTGTCTGA TAATTCTTCT 
AGATCAGTTT CTGAAGATGA GAAGCCCAAA GAAGTCTCTT CTGATTCTGC CCAACCTGTA 
TCTGAGGATG CAAGTCACAA GCGAGTATCC TCTAATTCTC CTAAACCAGC TTCTGAGGAT 
GTGAAACCAG CCTACTGGAC TCAGCCCCAT TTGGTCACAG GATACTGTAC ATACCTCCCC 
TTTCGGGGAT TTGACATTAC CCAGACACCA CCAACATACA TAAATGTGTT ACCAACTTTG 
CCCCAGTACA CTAACATTTA CACACCTCTG CCCAATGTTT CTTCTGAATA TCAGCTGCAA 
AGATCAGTGC CAGTGGTGCC GTCTTTTATA CCCAGTGAAA GACTAGATGA AAGTGCTACT 
GTGTACTTTG AGGGTCATCA TTTGAATGCT GAGAATGCCC CTGGGAACCA GATTGTCCCT 
AAAACACAGA TCCTTCAGGA CTCTATGGAA GTGTCAGTAA AGACAGAGTG CAGCACAGAT 
GGTGCTGATA CAGCCCTTAG TGAGTCTAAC AGATATGATG GTCACTGTGG AAATTCTGCC 
AACAAGTGGG AAGTAAATCC AGAAAAAAAC AATGAAGTAA CAAATACTCC ACATACACAA 
ATGGTTGCCA TACAGGTGTC TTGGAATATA ACACATCAAG AAGTCAATAC TGAGCCATAT 
GATCCTTTTG AGACACAACA GGGAGATATT TCACAGATTG AGAAGGAGTT CCAGGTTTTA 
CAAGAGCAGC TTAACGAGGC ATGTGAAAAT TATGAGCAGA GAAAACTCAA GGGCTCAGAA 
GAGACCAGGG ATCTGGAAGA AAAATTGAAA AGGAACTTAG AAGAAAACAA GATCTCAAAG 
ACAGAATTAG ATTGGTTCCT TGAAGACTTG GAAAAGGAAA TCAAGAAATG GCAACAGGAG 
AAAAAAGAAA TCCAAGAAAG TCTGAAAGCG CTAACAAAGA AAATGAGAAA GGTTTTAAAT 
GCCAACGAGA TATATGCCCA GAAAAATGAT GGAAAGGAAA AGGAACATGA ATTACTTCTG 
GATCAGTCCC TTGAAATCAG TAATACGTTT ACAAATGAGA AAATGAAAGT AGAAGAATGT 
ATAAAGAAAG GGAAGGAGAA TTATGAAGAG AGTCTTCAAA GAGCTGTGGC TGCAGAGGTA 
TCTGTGCTTG AAAACTGGAA GGACATTGAA GTGTACAAAT TACAGATCAT GGAATCACAA 
GCAGAAGGGT ATCTTAAGAA CCTGAGGCTA CTGAGCAGTG ATTCTGCAAC TTACCCTGAT 
AAGGAATCTG ATATACATTC ATGGGAATCA TTTCTTTCTA AGGTTAAAAA AGAAATTGAA 
AAAGCAAAGT CTCAGTATGA AGAACAAATT CAAGCAATTA AAAATGGCTC TCGACTCAGT 
GAACTTTCTA AAGTGCAAGT TCCTGAGCTT TCATTTCCTG TCTGTAGCAC AATTCTTCCT 
ACATCACTTC CTGAGTCTTC AGGCCATGAA GATCACAGAG CCTCCATTTC TACAAGTCAT 
GAGGCTGGAA ACCAAACAGC AGTTCCTAAG GACTCAAGTC TGGTCTCTGC CAGTGACGGC 
CCAGTGGGGG CTTCCTCTCC ACCATTGACA GGATCGCTCT CCTGTCAGCC CGAAGTCACC 
CAGCTGCCAG AGTGTAAGAG CACAGGCCAG GAAACTCCGC CAAAACAAAG CCTTGTGGCT 
GATCAGAAAC TACCTGTTCT TCCAGGACGT GCTACCCGCT CAAGCCAGTC TCCAAAAAAG 
CCGTTCAACA GTATTATTGA GCACCTGTCA GTGGTATTCC CATGTTACAA CAGCACTGAG 
CTTGCTGGTT TTATTAAAAA AGTGCGAAAC AAGAACAAAA ACTCGCTCTC AGGATTGAGT 
ATTGATGAAA TTGTCCAAAG AGTGACAGAA CACATTCTAG ATGAACAGAA AAAGAAAAAG 
CCAAATTCAA ATTCAGCAAA GGACAAGAGG TTGTCTGAGC CCAGCTCCAC TGCTTCTGTG 
AACAGGGCCT CCCAGGGTCC ACCCTCCGTG ACTGCTGGAC CATCGCCCAA AACCAAGGGA 
CAAAAGACAG AAGATGCCCC TGCATCGGGT GCAGCTTCCT GTGAACTGTG CCATGAGATA 
TTCAAATCCA AAAACGTGCG TGTACTAAAA TGTGGGCACA AGTTTCACAA AGGGTGCTTT 
AAGCAGTGGC TGAAGGGGCA GAGCACTTGC CCGGCCTGCC TCGATCAGGA TCTCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638963.1
CDS	1938..7937
Translation	MDDFALGDFTIADDALSEDCPYVDGCVFAPEFMSNDYVRVTQLYCDGVGMQYKDFTQSERNLEFDICNMWCSKPVSVLQDYCDAIKIYIFWPLLFQRQHSSIISRLHPCVEATNSHASEISLKKLQHLELMEDIVDLAKKVVNDSFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILPKLGAVDNCWPMLSIFFTEYKYHITKVVTENCNLLEEFKTQNCAECIEQGELMKMKGNEEFSKERFDIAIIYYTRAIEYRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKSNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPTRNPIKAFYESRAYIPSLSAPAFSTSLNFVETEKDSRKTNREMANGGNQNLKVVDEALKVDDCDCHPEFLPPSGQTPKYKGKQKSRNNELEKFSSSSQGSLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFIEECRFPPVPDAICCYQKCRGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKIQKKEAKKLAQERMEEDLRESNPPKTEEQKETVDSVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLSTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRIFLHVLSELKEVEPKLAAWIQKLNSFGLDATGPFFSRYGASLKELDFSIMTFLWNEKYGHKLASIEGKQLDYFCEPTSVKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSAKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETITSGEEHKRNSNSAGPFVVPDHLRQDVEEFEAIYEQHSSEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKSFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPMSDLSSATASENVKPKPVAANSPKSTCEVMKPRPVSDNSSRSVSEDEKPKEVSSDSAQPVSEDASHKRVSSNSPKPASEDVKPAYWTQPHLVTGYCTYLPFRGFDITQTPPTYINVLPTLPQYTNIYTPLPNVSSEYQLQRSVPVVPSFIPSERLDESATVYFEGHHLNAENAPGNQIVPKTQILQDSMEVSVKTECSTDGADTALSESNRYDGHCGNSANKWEVNPEKNNEVTNTPHTQMVAIQVSWNITHQEVNTEPYDPFETQQGDISQIEKEFQVLQEQLNEACENYEQRKLKGSEETRDLEEKLKRNLEENKISKTELDWFLEDLEKEIKKWQQEKKEIQESLKALTKKMRKVLNANEIYAQKNDGKEKEHELLLDQSLEISNTFTNEKMKVEECIKKGKENYEESLQRAVAAEVSVLENWKDIEVYKLQIMESQAEGYLKNLRLLSSDSATYPDKESDIHSWESFLSKVKKEIEKAKSQYEEQIQAIKNGSRLSELSKVQVPELSFPVCSTILPTSLPESSGHEDHRASISTSHEAGNQTAVPKDSSLVSASDGPVGASSPPLTGSLSCQPEVTQLPECKSTGQETPPKQSLVADQKLPVLPGRATRSSQSPKKPFNSIIEHLSVVFPCYNSTELAGFIKKVRNKNKNSLSGLSIDEIVQRVTEHILDEQKKKKPNSNSAKDKRLSEPSSTASVNRASQGPPSVTAGPSPKTKGQKTEDAPASGAASCELCHEIFKSKNVRVLKCGHKFHKGCFKQWLKGQSTCPACLDQDLL

Target ORF information:

RefSeq Version	XM_005638906.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638906.2

ORF Insert Sequence:

ATGGATGATT TTGCCCTGGG AGATTTCACT ATAGCAGATG ATGCTTTGTC AGAAGATTGC 
CCTTATGTGG ACGGTTGTGT CTTTGCTCCT GAATTTATGT CCAATGATTA TGTTCGTGTG 
ACTCAACTTT ACTGCGATGG GGTGGGTATG CAGTATAAAG ATTTTACCCA AAGTGAGAGA 
AACTTAGAAT TTGACATCTG TAATATGTGG TGTAGTAAAC CAGTTTCTGT CCTGCAAGAT 
TATTGTGATG CCATTAAAAT ATACATCTTC TGGCCACTTC TGTTTCAACG TCAGCATAGT 
TCTATAATAT CACGGTTACA CCCCTGTGTG GAGGCCACCA ACTCTCATGC TTCTGAGATA 
AGTTTGAAGA AATTGCAACA TCTAGAATTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTCGTGAATG ATTCATTCTT TATTGGGGGC TTATTGAGGA TTGGTTATAA AATAGAAAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT ATACAGGCGA TGTAACAATT 
CTACCTAAAT TAGGAGCAGT TGACAATTGT TGGCCTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AGTTGTAACA GAAAACTGCA ATTTGCTTGA AGAATTTAAA 
ACTCAAAATT GTGCGGAATG TATAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCAA AAGAAAGATT TGATATAGCT ATTATCTATT ACACCAGAGC CATTGAATAT 
AGACCTGAAA ATCACCTTCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG CACTGGACAG 
TTTAGAAATG CACTTGGTGA TGGAAAGAGA GCCACTATCC TGAAGAGCAA TTGGACGAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCGAAC ACCAACTAGA 
AATCCAATTA AAGCCTTTTA TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAGATT CCAGAAAAAC TAACCGCGAA 
ATGGCAAACG GTGGTAATCA GAATCTAAAG GTGGTAGATG AAGCTTTGAA GGTAGATGAT 
TGTGACTGTC ATCCTGAATT TCTACCACCA TCAGGTCAGA CTCCAAAATA CAAAGGAAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGTTCTA GTTCACAGGG GAGTTTACCA 
GTAGATTTGA AGAACATTTT GGAAAAACAA TTCTCTAAAT CTTCCAGAGC TGCACATCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGTTA CACGGCTTTG 
TTGGAGCAGC GTTGCCGCAG TGCTGCACAG GCCTTCACAG AATTGCTCAA TGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAACCTGGCC ATGATTAACT ATGTCTTAGT AGTCTACGGA 
CTTGCTATTT CTCTCCTTGG AATAGGACAG CCTGAGGAAT TATCTGAAGC TGAAAACCAG 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGTTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAGAACAGA TTTCTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATTTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGATT 
ATTGAAGAGA CTCAGCCGGG GAAAATAAAG ATGCTGTTAG AGAAGTTTAT TGAAGAATGC 
AGGTTCCCTC CAGTGCCAGA TGCCATTTGT TGCTATCAGA AGTGCCGTGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGAATCAG CTGCTGCCAG 
TACTGTAAAA TAGAATTTCA CATGAACTGT TGGAAAAAGT TGAAAACAAC AACTTTTAAT 
GATAAAATTG ACAAGGATTT TCTACAAGGA ATTTGTCTTA CCCCTGACTG TGAAGGAATC 
ATTTCTAAGA TTATCATTTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATAAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGCTAAGAC TGAAAGAAGA CAAAAAATTG AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA 
AAATTAGCAC AAGAAAGAAT GGAAGAGGAC CTAAGGGAAA GTAATCCACC CAAAACTGAA 
GAGCAGAAAG AAACTGTAGA CAGTGTTCAG AGTTGCCAGT TCCTTGACGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATA AAATCTGGTA TTCTGAACAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA AGCACAGAAG ACTACACAAC CTGTTTTTCT 
AGCAGAAATT TTCTAAATGA AGCGGTGGAC TACGTCATTC GTCACTTGAT TCAAGAAAAG 
AATAGAGTAA AGACTAGGAT ATTTCTGCAT GTTTTGAGCG AACTTAAAGA AGTGGAGCCA 
AAATTAGCCG CTTGGATCCA GAAACTTAAC AGCTTTGGTT TAGATGCCAC AGGACCTTTT 
TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA ACTAGCCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTGT 
GAGCCAACAT CAGTGAAGGA AGCACGGTGT TTAATATGGC TGCTAGAAGA GCATAGAGAC 
AAGTTCCCAG CGCTACACAA TGCTCTAGAT GAGTTCTTTG ACATAATGGA TAGCCGCTGT 
ACTGTATTAA GGAAACAAGA CAGTGGCGAT GTGCCGTTTA ATTCCGCCAA GATAAAAAAT 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TGGTGGGGTC TGGGACACCT 
TCTGTAACAC CAAATAATGA GACTATCACT TCGGGTGAAG AACATAAACG CAATTCAAAT 
TCTGCAGGGC CATTTGTGGT ACCTGACCAC CTTCGGCAAG ATGTAGAAGA ATTTGAAGCT 
ATCTATGAGC AGCACAGTAG TGAATATGTT GTCCGTAATA AAAAGCTGTG GGACATTAAC 
CCAAAACAAA AATGTTCAAC TCTGTATGAT TATTTCTCTC AGTTGTTGGA GGAGCATGGT 
CCCTTGGACA TGAGTAATAA AATGTTCTCT GAAGAATATG AATTCTTCCC AGAAGAAACT 
AGACAGATAC TAGAAAAAGC TGGAGGTTTG AAATCTTTCC TCCTGGGATG TCCTCGTTTT 
GTTGTGATTG ACAACTGTAT TGCTTTGAAG AAAGTTGCTT TACGGCTCAA GAAAAAGAGA 
AAGAAGAAAA ACATTAAGAC AAAAGTGGAA GAAATTTCAA AAACAGGGGA GTATTTACGA 
GTTAAACTAC CACTGAATCC AACTGCTAGG GAATTTAAAC CAGATGTAAA GTCCAAACCA 
ATGTCTGATT TATCTTCAGC AACAGCTTCT GAGAATGTGA AGCCCAAACC TGTAGCTGCT 
AATTCTCCCA AATCAACCTG TGAAGTTATG AAGCCAAGAC CAGTGTCTGA TAATTCTTCT 
AGATCAGTTT CTGAAGATGA GAAGCCCAAA GAAGTCTCTT CTGATTCTGC CCAACCTGTA 
TCTGAGGATG CAAGTCACAA GCGAGTATCC TCTAATTCTC CTAAACCAGC TTCTGAGGAT 
GTGAAACCAG CCTACTGGAC TCAGCCCCAT TTGGTCACAG GATACTGTAC ATACCTCCCC 
TTTCGGGGAT TTGACATTAC CCAGACACCA CCAACATACA TAAATGTGTT ACCAACTTTG 
CCCCAGTACA CTAACATTTA CACACCTCTG CCCAATGTTT CTTCTGAATA TCAGCTGCAA 
AGATCAGTGC CAGTGGTGCC GTCTTTTATA CCCAGTGAAA GACTAGATGA AAGTGCTACT 
GTGTACTTTG AGGGTCATCA TTTGAATGCT GAGAATGCCC CTGGGAACCA GATTGTCCCT 
AAAACACAGA TCCTTCAGGA CTCTATGGAA GTGTCAGTAA AGACAGAGTG CAGCACAGAT 
GGTGCTGATA CAGCCCTTAG TGAGTCTAAC AGATATGATG GTCACTGTGG AAATTCTGCC 
AACAAGTGGG AAGTAAATCC AGAAAAAAAC AATGAAGTAA CAAATACTCC ACATACACAA 
ATGGTTGCCA TACAGGTGTC TTGGAATATA ACACATCAAG AAGTCAATAC TGAGCCATAT 
GATCCTTTTG AGACACAACA GGGAGATATT TCACAGATTG AGAAGGAGTT CCAGGTTTTA 
CAAGAGCAGC TTAACGAGGC ATGTGAAAAT TATGAGCAGA GAAAACTCAA GGGCTCAGAA 
GAGACCAGGG ATCTGGAAGA AAAATTGAAA AGGAACTTAG AAGAAAACAA GATCTCAAAG 
ACAGAATTAG ATTGGTTCCT TGAAGACTTG GAAAAGGAAA TCAAGAAATG GCAACAGGAG 
AAAAAAGAAA TCCAAGAAAG TCTGAAAGCG CTAACAAAGA AAATGAGAAA GGTTTTAAAT 
GCCAACGAGA TATATGCCCA GAAAAATGAT GGAAAGGAAA AGGAACATGA ATTACTTCTG 
GATCAGTCCC TTGAAATCAG TAATACGTTT ACAAATGAGA AAATGAAAGT AGAAGAATGT 
ATAAAGAAAG GGAAGGAGAA TTATGAAGAG AGTCTTCAAA GAGCTGTGGC TGCAGAGGTA 
TCTGTGCTTG AAAACTGGAA GGACATTGAA GTGTACAAAT TACAGATCAT GGAATCACAA 
GCAGAAGGGT ATCTTAAGAA CCTGAGGCTA CTGAGCAGTG ATTCTGCAAC TTACCCTGAT 
AAGGAATCTG ATATACATTC ATGGGAATCA TTTCTTTCTA AGGTTAAAAA AGAAATTGAA 
AAAGCAAAGT CTCAGTATGA AGAACAAATT CAAGCAATTA AAAATGGCTC TCGACTCAGT 
GAACTTTCTA AAGTGCAAGT TCCTGAGCTT TCATTTCCTG TCTGTAGCAC AATTCTTCCT 
ACATCACTTC CTGAGTCTTC AGGCCATGAA GATCACAGAG CCTCCATTTC TACAAGTCAT 
GAGGCTGGAA ACCAAACAGC AGTTCCTAAG GACTCAAGTC TGGTCTCTGC CAGTGACGGC 
CCAGTGGGGG CTTCCTCTCC ACCATTGACA GGATCGCTCT CCTGTCAGCC CGAAGTCACC 
CAGCTGCCAG AGTGTAAGAG CACAGGCCAG GAAACTCCGC CAAAACAAAG CCTTGTGGCT 
GATCAGAAAC TACCTGTTCT TCCAGGACGT GCTACCCGCT CAAGCCAGTC TCCAAAAAAG 
CCGTTCAACA GTATTATTGA GCACCTGTCA GTGGTATTCC CATGTTACAA CAGCACTGAG 
CTTGCTGGTT TTATTAAAAA AGTGCGAAAC AAGAACAAAA ACTCGCTCTC AGGATTGAGT 
ATTGATGAAA TTGTCCAAAG AGTGACAGAA CACATTCTAG ATGAACAGAA AAAGAAAAAG 
CCAAATTCAA ATTCAGCAAA GGACAAGAGG TTGTCTGAGC CCAGCTCCAC TGCTTCTGTG 
AACAGGGCCT CCCAGGGTCC ACCCTCCGTG ACTGCTGGAC CATCGCCCAA AACCAAGGGA 
CAAAAGACAG AAGATGCCCC TGCATCGGGT GCAGCTTCCT GTGAACTGTG CCATGAGATA 
TTCAAATCCA AAAACGTGCG TGTACTAAAA TGTGGGCACA AGTTTCACAA AGGGTGCTTT 
AAGCAGTGGC TGAAGGGGCA GAGCACTTGC CCGGCCTGCC TCGATCAGGA TCTCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005638907.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638964.1 Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

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CloneID	OCe29427
Clone ID Related Accession (Same CDS sequence)	XM_005638907.2 , XM_005638907.3
Accession Version	XM_005638907.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638907.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCGAAC ACCAACTAGA AATCCAATTA AAGCCTTTTA TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAGATT CCAGAAAAAC TAACCGCGAA ATGGCAAACG GTGGTAATCA GAATCTAAAG 
GTGGTAGATG AAGCTTTGAA GGTAGATGAT TGTGACTGTC ATCCTGAATT TCTACCACCA 
TCAGGTCAGA CTCCAAAATA CAAAGGAAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGTTCTA GTTCACAGGG GAGTTTACCA GTAGATTTGA AGAACATTTT GGAAAAACAA 
TTCTCTAAAT CTTCCAGAGC TGCACATCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGTTA CACGGCTTTG TTGGAGCAGC GTTGCCGCAG TGCTGCACAG 
GCCTTCACAG AATTGCTCAA TGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC 
ATGATTAACT ATGTCTTAGT AGTCTACGGA CTTGCTATTT CTCTCCTTGG AATAGGACAG 
CCTGAGGAAT TATCTGAAGC TGAAAACCAG TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGTTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAGAACAGA 
TTTCTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATTTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGATT ATTGAAGAGA CTCAGCCGGG GAAAATAAAG 
ATGCTGTTAG AGAAGTTTAT TGAAGAATGC AGGTTCCCTC CAGTGCCAGA TGCCATTTGT 
TGCTATCAGA AGTGCCGTGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGAATCAG CTGCTGCCAG TACTGTAAAA TAGAATTTCA CATGAACTGT 
TGGAAAAAGT TGAAAACAAC AACTTTTAAT GATAAAATTG ACAAGGATTT TCTACAAGGA 
ATTTGTCTTA CCCCTGACTG TGAAGGAATC ATTTCTAAGA TTATCATTTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATAAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGCTAAGAC TGAAAGAAGA CAAAAAATTG 
AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA AAATTAGCAC AAGAAAGAAT GGAAGAGGAC 
CTAAGGGAAA GTAATCCACC CAAAACTGAA GAGCAGAAAG AAACTGTAGA CAGTGTTCAG 
AGTTGCCAGT TCCTTGACGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATA 
AAATCTGGTA TTCTGAACAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
AGCACAGAAG ACTACACAAC CTGTTTTTCT AGCAGAAATT TTCTAAATGA AGCGGTGGAC 
TACGTCATTC GTCACTTGAT TCAAGAAAAG AATAGAGTAA AGACTAGGAT ATTTCTGCAT 
GTTTTGAGCG AACTTAAAGA AGTGGAGCCA AAATTAGCCG CTTGGATCCA GAAACTTAAC 
AGCTTTGGTT TAGATGCCAC AGGACCTTTT TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA ACTAGCCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTGT GAGCCAACAT CAGTGAAGGA AGCACGGTGT 
TTAATATGGC TGCTAGAAGA GCATAGAGAC AAGTTCCCAG CGCTACACAA TGCTCTAGAT 
GAGTTCTTTG ACATAATGGA TAGCCGCTGT ACTGTATTAA GGAAACAAGA CAGTGGCGAT 
GTGCCGTTTA ATTCCGCCAA GATAAAAAAT AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TGGTGGGGTC TGGGACACCT TCTGTAACAC CAAATAATGA GACTATCACT 
TCGGGTGAAG AACATAACAG ACGCAATTCA AATTCTGCAG GGCCATTTGT GGTACCTGAC 
CACCTTCGGC AAGATGTAGA AGAATTTGAA GCTATCTATG AGCAGCACAG TAGTGAATAT 
GTTGTCCGTA ATAAAAAGCT GTGGGACATT AACCCAAAAC AAAAATGTTC AACTCTGTAT 
GATTATTTCT CTCAGTTGTT GGAGGAGCAT GGTCCCTTGG ACATGAGTAA TAAAATGTTC 
TCTGAAGAAT ATGAATTCTT CCCAGAAGAA ACTAGACAGA TACTAGAAAA AGCTGGAGGT 
TTGAAATCTT TCCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCTTTG 
AAGAAAGTTG CTTTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTG 
GAAGAAATTT CAAAAACAGG GGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAATGTCTG ATTTATCTTC AGCAACAGCT 
TCTGAGAATG TGAAGCCCAA ACCTGTAGCT GCTAATTCTC CCAAATCAAC CTGTGAAGTT 
ATGAAGCCAA GACCAGTGTC TGATAATTCT TCTAGATCAG TTTCTGAAGA TGAGAAGCCC 
AAAGAAGTCT CTTCTGATTC TGCCCAACCT GTATCTGAGG ATGCAAGTCA CAAGCGAGTA 
TCCTCTAATT CTCCTAAACC AGCTTCTGAG GATGTGAAAC CAGCCTACTG GACTCAGCCC 
CATTTGGTCA CAGGATACTG TACATACCTC CCCTTTCGGG GATTTGACAT TACCCAGACA 
CCACCAACAT ACATAAATGT GTTACCAACT TTGCCCCAGT ACACTAACAT TTACACACCT 
CTGCCCAATG TTTCTTCTGA ATATCAGCTG CAAAGATCAG TGCCAGTGGT GCCGTCTTTT 
ATACCCAGTG AAAGACTAGA TGAAAGTGCT ACTGTGTACT TTGAGGGTCA TCATTTGAAT 
GCTGAGAATG CCCCTGGGAA CCAGATTGTC CCTAAAACAC AGATCCTTCA GGACTCTATG 
GAAGTGTCAG TAAAGACAGA GTGCAGCACA GATGGTGCTG ATACAGCCCT TAGTGAGTCT 
AACAGATATG ATGGTCACTG TGGAAATTCT GCCAACAAGT GGGAAGTAAA TCCAGAAAAA 
AACAATGAAG TAACAAATAC TCCACATACA CAAATGGTTG CCATACAGGT GTCTTGGAAT 
ATAACACATC AAGAAGTCAA TACTGAGCCA TATGATCCTT TTGAGACACA ACAGGGAGAT 
ATTTCACAGA TTGAGAAGGA GTTCCAGGTT TTACAAGAGC AGCTTAACGA GGCATGTGAA 
AATTATGAGC AGAGAAAACT CAAGGGCTCA GAAGAGACCA GGGATCTGGA AGAAAAATTG 
AAAAGGAACT TAGAAGAAAA CAAGATCTCA AAGACAGAAT TAGATTGGTT CCTTGAAGAC 
TTGGAAAAGG AAATCAAGAA ATGGCAACAG GAGAAAAAAG AAATCCAAGA AAGTCTGAAA 
GCGCTAACAA AGAAAATGAG AAAGGTTTTA AATGCCAACG AGATATATGC CCAGAAAAAT 
GATGGAAAGG AAAAGGAACA TGAATTACTT CTGGATCAGT CCCTTGAAAT CAGTAATACG 
TTTACAAATG AGAAAATGAA AGTAGAAGAA TGTATAAAGA AAGGGAAGGA GAATTATGAA 
GAGAGTCTTC AAAGAGCTGT GGCTGCAGAG GTATCTGTGC TTGAAAACTG GAAGGACATT 
GAAGTGTACA AATTACAGAT CATGGAATCA CAAGCAGAAG GGTATCTTAA GAACCTGAGG 
CTACTGAGCA GTGATTCTGC AACTTACCCT GATAAGGAAT CTGATATACA TTCATGGGAA 
TCATTTCTTT CTAAGGTTAA AAAAGAAATT GAAAAAGCAA AGTCTCAGTA TGAAGAACAA 
ATTCAAGCAA TTAAAAATGG CTCTCGACTC AGTGAACTTT CTAAAGTGCA AGTTCCTGAG 
CTTTCATTTC CTGTCTGTAG CACAATTCTT CCTACATCAC TTCCTGAGTC TTCAGGCCAT 
GAAGATCACA GAGCCTCCAT TTCTACAAGT CATGAGGCTG GAAACCAAAC AGCAGTTCCT 
AAGGACTCAA GTCTGGTCTC TGCCAGTGAC GGCCCAGTGG GGGCTTCCTC TCCACCATTG 
ACAGGATCGC TCTCCTGTCA GCCCGAAGTC ACCCAGCTGC CAGAGTGTAA GAGCACAGGC 
CAGGAAACTC CGCCAAAACA AAGCCTTGTG GCTGATCAGA AACTACCTGT TCTTCCAGGA 
CGTGCTACCC GCTCAAGCCA GTCTCCAAAA AAGCCGTTCA ACAGTATTAT TGAGCACCTG 
TCAGTGGTAT TCCCATGTTA CAACAGCACT GAGCTTGCTG GTTTTATTAA AAAAGTGCGA 
AACAAGAACA AAAACTCGCT CTCAGGATTG AGTATTGATG AAATTGTCCA AAGAGTGACA 
GAACACATTC TAGATGAACA GAAAAAGAAA AAGCCAAATT CAAATTCAGC AAAGGACAAG 
AGGTTGTCTG AGCCCAGCTC CACTGCTTCT GTGAACAGGG CCTCCCAGGG TCCACCCTCC 
GTGACTGCTG GACCATCGCC CAAAACCAAG GGACAAAAGA CAGAAGATGC CCCTGCATCG 
GGTGCAGCTT CCTGTGAACT GTGCCATGAG ATATTCAAAT CCAAAAACGT GCGTGTACTA 
AAATGTGGGC ACAAGTTTCA CAAAGGGTGC TTTAAGCAGT GGCTGAAGGG GCAGAGCACT 
TGCCCGGCCT GCCTCGATCA GGATCTCCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638964.1
CDS	1513..6585
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPTRNPIKAFYESRAYIPSLSAPAFSTSLNFVETEKDSRKTNREMANGGNQNLKVVDEALKVDDCDCHPEFLPPSGQTPKYKGKQKSRNNELEKFSSSSQGSLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFIEECRFPPVPDAICCYQKCRGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKIQKKEAKKLAQERMEEDLRESNPPKTEEQKETVDSVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLSTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRIFLHVLSELKEVEPKLAAWIQKLNSFGLDATGPFFSRYGASLKELDFSIMTFLWNEKYGHKLASIEGKQLDYFCEPTSVKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSAKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETITSGEEHNRRNSNSAGPFVVPDHLRQDVEEFEAIYEQHSSEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKSFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPMSDLSSATASENVKPKPVAANSPKSTCEVMKPRPVSDNSSRSVSEDEKPKEVSSDSAQPVSEDASHKRVSSNSPKPASEDVKPAYWTQPHLVTGYCTYLPFRGFDITQTPPTYINVLPTLPQYTNIYTPLPNVSSEYQLQRSVPVVPSFIPSERLDESATVYFEGHHLNAENAPGNQIVPKTQILQDSMEVSVKTECSTDGADTALSESNRYDGHCGNSANKWEVNPEKNNEVTNTPHTQMVAIQVSWNITHQEVNTEPYDPFETQQGDISQIEKEFQVLQEQLNEACENYEQRKLKGSEETRDLEEKLKRNLEENKISKTELDWFLEDLEKEIKKWQQEKKEIQESLKALTKKMRKVLNANEIYAQKNDGKEKEHELLLDQSLEISNTFTNEKMKVEECIKKGKENYEESLQRAVAAEVSVLENWKDIEVYKLQIMESQAEGYLKNLRLLSSDSATYPDKESDIHSWESFLSKVKKEIEKAKSQYEEQIQAIKNGSRLSELSKVQVPELSFPVCSTILPTSLPESSGHEDHRASISTSHEAGNQTAVPKDSSLVSASDGPVGASSPPLTGSLSCQPEVTQLPECKSTGQETPPKQSLVADQKLPVLPGRATRSSQSPKKPFNSIIEHLSVVFPCYNSTELAGFIKKVRNKNKNSLSGLSIDEIVQRVTEHILDEQKKKKPNSNSAKDKRLSEPSSTASVNRASQGPPSVTAGPSPKTKGQKTEDAPASGAASCELCHEIFKSKNVRVLKCGHKFHKGCFKQWLKGQSTCPACLDQDLL

Target ORF information:

RefSeq Version	XM_005638907.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638907.2

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCGAAC ACCAACTAGA AATCCAATTA AAGCCTTTTA TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAGATT CCAGAAAAAC TAACCGCGAA ATGGCAAACG GTGGTAATCA GAATCTAAAG 
GTGGTAGATG AAGCTTTGAA GGTAGATGAT TGTGACTGTC ATCCTGAATT TCTACCACCA 
TCAGGTCAGA CTCCAAAATA CAAAGGAAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGTTCTA GTTCACAGGG GAGTTTACCA GTAGATTTGA AGAACATTTT GGAAAAACAA 
TTCTCTAAAT CTTCCAGAGC TGCACATCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGTTA CACGGCTTTG TTGGAGCAGC GTTGCCGCAG TGCTGCACAG 
GCCTTCACAG AATTGCTCAA TGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC 
ATGATTAACT ATGTCTTAGT AGTCTACGGA CTTGCTATTT CTCTCCTTGG AATAGGACAG 
CCTGAGGAAT TATCTGAAGC TGAAAACCAG TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGTTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAGAACAGA 
TTTCTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATTTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGATT ATTGAAGAGA CTCAGCCGGG GAAAATAAAG 
ATGCTGTTAG AGAAGTTTAT TGAAGAATGC AGGTTCCCTC CAGTGCCAGA TGCCATTTGT 
TGCTATCAGA AGTGCCGTGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGAATCAG CTGCTGCCAG TACTGTAAAA TAGAATTTCA CATGAACTGT 
TGGAAAAAGT TGAAAACAAC AACTTTTAAT GATAAAATTG ACAAGGATTT TCTACAAGGA 
ATTTGTCTTA CCCCTGACTG TGAAGGAATC ATTTCTAAGA TTATCATTTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATAAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGCTAAGAC TGAAAGAAGA CAAAAAATTG 
AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA AAATTAGCAC AAGAAAGAAT GGAAGAGGAC 
CTAAGGGAAA GTAATCCACC CAAAACTGAA GAGCAGAAAG AAACTGTAGA CAGTGTTCAG 
AGTTGCCAGT TCCTTGACGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATA 
AAATCTGGTA TTCTGAACAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
AGCACAGAAG ACTACACAAC CTGTTTTTCT AGCAGAAATT TTCTAAATGA AGCGGTGGAC 
TACGTCATTC GTCACTTGAT TCAAGAAAAG AATAGAGTAA AGACTAGGAT ATTTCTGCAT 
GTTTTGAGCG AACTTAAAGA AGTGGAGCCA AAATTAGCCG CTTGGATCCA GAAACTTAAC 
AGCTTTGGTT TAGATGCCAC AGGACCTTTT TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA ACTAGCCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTGT GAGCCAACAT CAGTGAAGGA AGCACGGTGT 
TTAATATGGC TGCTAGAAGA GCATAGAGAC AAGTTCCCAG CGCTACACAA TGCTCTAGAT 
GAGTTCTTTG ACATAATGGA TAGCCGCTGT ACTGTATTAA GGAAACAAGA CAGTGGCGAT 
GTGCCGTTTA ATTCCGCCAA GATAAAAAAT AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TGGTGGGGTC TGGGACACCT TCTGTAACAC CAAATAATGA GACTATCACT 
TCGGGTGAAG AACATAACAG ACGCAATTCA AATTCTGCAG GGCCATTTGT GGTACCTGAC 
CACCTTCGGC AAGATGTAGA AGAATTTGAA GCTATCTATG AGCAGCACAG TAGTGAATAT 
GTTGTCCGTA ATAAAAAGCT GTGGGACATT AACCCAAAAC AAAAATGTTC AACTCTGTAT 
GATTATTTCT CTCAGTTGTT GGAGGAGCAT GGTCCCTTGG ACATGAGTAA TAAAATGTTC 
TCTGAAGAAT ATGAATTCTT CCCAGAAGAA ACTAGACAGA TACTAGAAAA AGCTGGAGGT 
TTGAAATCTT TCCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCTTTG 
AAGAAAGTTG CTTTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTG 
GAAGAAATTT CAAAAACAGG GGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAATGTCTG ATTTATCTTC AGCAACAGCT 
TCTGAGAATG TGAAGCCCAA ACCTGTAGCT GCTAATTCTC CCAAATCAAC CTGTGAAGTT 
ATGAAGCCAA GACCAGTGTC TGATAATTCT TCTAGATCAG TTTCTGAAGA TGAGAAGCCC 
AAAGAAGTCT CTTCTGATTC TGCCCAACCT GTATCTGAGG ATGCAAGTCA CAAGCGAGTA 
TCCTCTAATT CTCCTAAACC AGCTTCTGAG GATGTGAAAC CAGCCTACTG GACTCAGCCC 
CATTTGGTCA CAGGATACTG TACATACCTC CCCTTTCGGG GATTTGACAT TACCCAGACA 
CCACCAACAT ACATAAATGT GTTACCAACT TTGCCCCAGT ACACTAACAT TTACACACCT 
CTGCCCAATG TTTCTTCTGA ATATCAGCTG CAAAGATCAG TGCCAGTGGT GCCGTCTTTT 
ATACCCAGTG AAAGACTAGA TGAAAGTGCT ACTGTGTACT TTGAGGGTCA TCATTTGAAT 
GCTGAGAATG CCCCTGGGAA CCAGATTGTC CCTAAAACAC AGATCCTTCA GGACTCTATG 
GAAGTGTCAG TAAAGACAGA GTGCAGCACA GATGGTGCTG ATACAGCCCT TAGTGAGTCT 
AACAGATATG ATGGTCACTG TGGAAATTCT GCCAACAAGT GGGAAGTAAA TCCAGAAAAA 
AACAATGAAG TAACAAATAC TCCACATACA CAAATGGTTG CCATACAGGT GTCTTGGAAT 
ATAACACATC AAGAAGTCAA TACTGAGCCA TATGATCCTT TTGAGACACA ACAGGGAGAT 
ATTTCACAGA TTGAGAAGGA GTTCCAGGTT TTACAAGAGC AGCTTAACGA GGCATGTGAA 
AATTATGAGC AGAGAAAACT CAAGGGCTCA GAAGAGACCA GGGATCTGGA AGAAAAATTG 
AAAAGGAACT TAGAAGAAAA CAAGATCTCA AAGACAGAAT TAGATTGGTT CCTTGAAGAC 
TTGGAAAAGG AAATCAAGAA ATGGCAACAG GAGAAAAAAG AAATCCAAGA AAGTCTGAAA 
GCGCTAACAA AGAAAATGAG AAAGGTTTTA AATGCCAACG AGATATATGC CCAGAAAAAT 
GATGGAAAGG AAAAGGAACA TGAATTACTT CTGGATCAGT CCCTTGAAAT CAGTAATACG 
TTTACAAATG AGAAAATGAA AGTAGAAGAA TGTATAAAGA AAGGGAAGGA GAATTATGAA 
GAGAGTCTTC AAAGAGCTGT GGCTGCAGAG GTATCTGTGC TTGAAAACTG GAAGGACATT 
GAAGTGTACA AATTACAGAT CATGGAATCA CAAGCAGAAG GGTATCTTAA GAACCTGAGG 
CTACTGAGCA GTGATTCTGC AACTTACCCT GATAAGGAAT CTGATATACA TTCATGGGAA 
TCATTTCTTT CTAAGGTTAA AAAAGAAATT GAAAAAGCAA AGTCTCAGTA TGAAGAACAA 
ATTCAAGCAA TTAAAAATGG CTCTCGACTC AGTGAACTTT CTAAAGTGCA AGTTCCTGAG 
CTTTCATTTC CTGTCTGTAG CACAATTCTT CCTACATCAC TTCCTGAGTC TTCAGGCCAT 
GAAGATCACA GAGCCTCCAT TTCTACAAGT CATGAGGCTG GAAACCAAAC AGCAGTTCCT 
AAGGACTCAA GTCTGGTCTC TGCCAGTGAC GGCCCAGTGG GGGCTTCCTC TCCACCATTG 
ACAGGATCGC TCTCCTGTCA GCCCGAAGTC ACCCAGCTGC CAGAGTGTAA GAGCACAGGC 
CAGGAAACTC CGCCAAAACA AAGCCTTGTG GCTGATCAGA AACTACCTGT TCTTCCAGGA 
CGTGCTACCC GCTCAAGCCA GTCTCCAAAA AAGCCGTTCA ACAGTATTAT TGAGCACCTG 
TCAGTGGTAT TCCCATGTTA CAACAGCACT GAGCTTGCTG GTTTTATTAA AAAAGTGCGA 
AACAAGAACA AAAACTCGCT CTCAGGATTG AGTATTGATG AAATTGTCCA AAGAGTGACA 
GAACACATTC TAGATGAACA GAAAAAGAAA AAGCCAAATT CAAATTCAGC AAAGGACAAG 
AGGTTGTCTG AGCCCAGCTC CACTGCTTCT GTGAACAGGG CCTCCCAGGG TCCACCCTCC 
GTGACTGCTG GACCATCGCC CAAAACCAAG GGACAAAAGA CAGAAGATGC CCCTGCATCG 
GGTGCAGCTT CCTGTGAACT GTGCCATGAG ATATTCAAAT CCAAAAACGT GCGTGTACTA 
AAATGTGGGC ACAAGTTTCA CAAAGGGTGC TTTAAGCAGT GGCTGAAGGG GCAGAGCACT 
TGCCCGGCCT GCCTCGATCA GGATCTCCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005638907.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638964.1 Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

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CloneID	OCe29427
Clone ID Related Accession (Same CDS sequence)	XM_005638907.2 , XM_005638907.3
Accession Version	XM_005638907.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCGAAC ACCAACTAGA AATCCAATTA AAGCCTTTTA TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAGATT CCAGAAAAAC TAACCGCGAA ATGGCAAACG GTGGTAATCA GAATCTAAAG 
GTGGTAGATG AAGCTTTGAA GGTAGATGAT TGTGACTGTC ATCCTGAATT TCTACCACCA 
TCAGGTCAGA CTCCAAAATA CAAAGGAAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGTTCTA GTTCACAGGG GAGTTTACCA GTAGATTTGA AGAACATTTT GGAAAAACAA 
TTCTCTAAAT CTTCCAGAGC TGCACATCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGTTA CACGGCTTTG TTGGAGCAGC GTTGCCGCAG TGCTGCACAG 
GCCTTCACAG AATTGCTCAA TGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC 
ATGATTAACT ATGTCTTAGT AGTCTACGGA CTTGCTATTT CTCTCCTTGG AATAGGACAG 
CCTGAGGAAT TATCTGAAGC TGAAAACCAG TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGTTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAGAACAGA 
TTTCTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATTTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGATT ATTGAAGAGA CTCAGCCGGG GAAAATAAAG 
ATGCTGTTAG AGAAGTTTAT TGAAGAATGC AGGTTCCCTC CAGTGCCAGA TGCCATTTGT 
TGCTATCAGA AGTGCCGTGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGAATCAG CTGCTGCCAG TACTGTAAAA TAGAATTTCA CATGAACTGT 
TGGAAAAAGT TGAAAACAAC AACTTTTAAT GATAAAATTG ACAAGGATTT TCTACAAGGA 
ATTTGTCTTA CCCCTGACTG TGAAGGAATC ATTTCTAAGA TTATCATTTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATAAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGCTAAGAC TGAAAGAAGA CAAAAAATTG 
AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA AAATTAGCAC AAGAAAGAAT GGAAGAGGAC 
CTAAGGGAAA GTAATCCACC CAAAACTGAA GAGCAGAAAG AAACTGTAGA CAGTGTTCAG 
AGTTGCCAGT TCCTTGACGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATA 
AAATCTGGTA TTCTGAACAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
AGCACAGAAG ACTACACAAC CTGTTTTTCT AGCAGAAATT TTCTAAATGA AGCGGTGGAC 
TACGTCATTC GTCACTTGAT TCAAGAAAAG AATAGAGTAA AGACTAGGAT ATTTCTGCAT 
GTTTTGAGCG AACTTAAAGA AGTGGAGCCA AAATTAGCCG CTTGGATCCA GAAACTTAAC 
AGCTTTGGTT TAGATGCCAC AGGACCTTTT TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA ACTAGCCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTGT GAGCCAACAT CAGTGAAGGA AGCACGGTGT 
TTAATATGGC TGCTAGAAGA GCATAGAGAC AAGTTCCCAG CGCTACACAA TGCTCTAGAT 
GAGTTCTTTG ACATAATGGA TAGCCGCTGT ACTGTATTAA GGAAACAAGA CAGTGGCGAT 
GTGCCGTTTA ATTCCGCCAA GATAAAAAAT AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TGGTGGGGTC TGGGACACCT TCTGTAACAC CAAATAATGA GACTATCACT 
TCGGGTGAAG AACATAACAG ACGCAATTCA AATTCTGCAG GGCCATTTGT GGTACCTGAC 
CACCTTCGGC AAGATGTAGA AGAATTTGAA GCTATCTATG AGCAGCACAG TAGTGAATAT 
GTTGTCCGTA ATAAAAAGCT GTGGGACATT AACCCAAAAC AAAAATGTTC AACTCTGTAT 
GATTATTTCT CTCAGTTGTT GGAGGAGCAT GGTCCCTTGG ACATGAGTAA TAAAATGTTC 
TCTGAAGAAT ATGAATTCTT CCCAGAAGAA ACTAGACAGA TACTAGAAAA AGCTGGAGGT 
TTGAAATCTT TCCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCTTTG 
AAGAAAGTTG CTTTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTG 
GAAGAAATTT CAAAAACAGG GGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAATGTCTG ATTTATCTTC AGCAACAGCT 
TCTGAGAATG TGAAGCCCAA ACCTGTAGCT GCTAATTCTC CCAAATCAAC CTGTGAAGTT 
ATGAAGCCAA GACCAGTGTC TGATAATTCT TCTAGATCAG TTTCTGAAGA TGAGAAGCCC 
AAAGAAGTCT CTTCTGATTC TGCCCAACCT GTATCTGAGG ATGCAAGTCA CAAGCGAGTA 
TCCTCTAATT CTCCTAAACC AGCTTCTGAG GATGTGAAAC CAGCCTACTG GACTCAGCCC 
CATTTGGTCA CAGGATACTG TACATACCTC CCCTTTCGGG GATTTGACAT TACCCAGACA 
CCACCAACAT ACATAAATGT GTTACCAACT TTGCCCCAGT ACACTAACAT TTACACACCT 
CTGCCCAATG TTTCTTCTGA ATATCAGCTG CAAAGATCAG TGCCAGTGGT GCCGTCTTTT 
ATACCCAGTG AAAGACTAGA TGAAAGTGCT ACTGTGTACT TTGAGGGTCA TCATTTGAAT 
GCTGAGAATG CCCCTGGGAA CCAGATTGTC CCTAAAACAC AGATCCTTCA GGACTCTATG 
GAAGTGTCAG TAAAGACAGA GTGCAGCACA GATGGTGCTG ATACAGCCCT TAGTGAGTCT 
AACAGATATG ATGGTCACTG TGGAAATTCT GCCAACAAGT GGGAAGTAAA TCCAGAAAAA 
AACAATGAAG TAACAAATAC TCCACATACA CAAATGGTTG CCATACAGGT GTCTTGGAAT 
ATAACACATC AAGAAGTCAA TACTGAGCCA TATGATCCTT TTGAGACACA ACAGGGAGAT 
ATTTCACAGA TTGAGAAGGA GTTCCAGGTT TTACAAGAGC AGCTTAACGA GGCATGTGAA 
AATTATGAGC AGAGAAAACT CAAGGGCTCA GAAGAGACCA GGGATCTGGA AGAAAAATTG 
AAAAGGAACT TAGAAGAAAA CAAGATCTCA AAGACAGAAT TAGATTGGTT CCTTGAAGAC 
TTGGAAAAGG AAATCAAGAA ATGGCAACAG GAGAAAAAAG AAATCCAAGA AAGTCTGAAA 
GCGCTAACAA AGAAAATGAG AAAGGTTTTA AATGCCAACG AGATATATGC CCAGAAAAAT 
GATGGAAAGG AAAAGGAACA TGAATTACTT CTGGATCAGT CCCTTGAAAT CAGTAATACG 
TTTACAAATG AGAAAATGAA AGTAGAAGAA TGTATAAAGA AAGGGAAGGA GAATTATGAA 
GAGAGTCTTC AAAGAGCTGT GGCTGCAGAG GTATCTGTGC TTGAAAACTG GAAGGACATT 
GAAGTGTACA AATTACAGAT CATGGAATCA CAAGCAGAAG GGTATCTTAA GAACCTGAGG 
CTACTGAGCA GTGATTCTGC AACTTACCCT GATAAGGAAT CTGATATACA TTCATGGGAA 
TCATTTCTTT CTAAGGTTAA AAAAGAAATT GAAAAAGCAA AGTCTCAGTA TGAAGAACAA 
ATTCAAGCAA TTAAAAATGG CTCTCGACTC AGTGAACTTT CTAAAGTGCA AGTTCCTGAG 
CTTTCATTTC CTGTCTGTAG CACAATTCTT CCTACATCAC TTCCTGAGTC TTCAGGCCAT 
GAAGATCACA GAGCCTCCAT TTCTACAAGT CATGAGGCTG GAAACCAAAC AGCAGTTCCT 
AAGGACTCAA GTCTGGTCTC TGCCAGTGAC GGCCCAGTGG GGGCTTCCTC TCCACCATTG 
ACAGGATCGC TCTCCTGTCA GCCCGAAGTC ACCCAGCTGC CAGAGTGTAA GAGCACAGGC 
CAGGAAACTC CGCCAAAACA AAGCCTTGTG GCTGATCAGA AACTACCTGT TCTTCCAGGA 
CGTGCTACCC GCTCAAGCCA GTCTCCAAAA AAGCCGTTCA ACAGTATTAT TGAGCACCTG 
TCAGTGGTAT TCCCATGTTA CAACAGCACT GAGCTTGCTG GTTTTATTAA AAAAGTGCGA 
AACAAGAACA AAAACTCGCT CTCAGGATTG AGTATTGATG AAATTGTCCA AAGAGTGACA 
GAACACATTC TAGATGAACA GAAAAAGAAA AAGCCAAATT CAAATTCAGC AAAGGACAAG 
AGGTTGTCTG AGCCCAGCTC CACTGCTTCT GTGAACAGGG CCTCCCAGGG TCCACCCTCC 
GTGACTGCTG GACCATCGCC CAAAACCAAG GGACAAAAGA CAGAAGATGC CCCTGCATCG 
GGTGCAGCTT CCTGTGAACT GTGCCATGAG ATATTCAAAT CCAAAAACGT GCGTGTACTA 
AAATGTGGGC ACAAGTTTCA CAAAGGGTGC TTTAAGCAGT GGCTGAAGGG GCAGAGCACT 
TGCCCGGCCT GCCTCGATCA GGATCTCCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638964.1
CDS	1503..6575
Translation	MLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPTRNPIKAFYESRAYIPSLSAPAFSTSLNFVETEKDSRKTNREMANGGNQNLKVVDEALKVDDCDCHPEFLPPSGQTPKYKGKQKSRNNELEKFSSSSQGSLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFIEECRFPPVPDAICCYQKCRGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKIQKKEAKKLAQERMEEDLRESNPPKTEEQKETVDSVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLSTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRIFLHVLSELKEVEPKLAAWIQKLNSFGLDATGPFFSRYGASLKELDFSIMTFLWNEKYGHKLASIEGKQLDYFCEPTSVKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSAKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETITSGEEHNRRNSNSAGPFVVPDHLRQDVEEFEAIYEQHSSEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKSFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPMSDLSSATASENVKPKPVAANSPKSTCEVMKPRPVSDNSSRSVSEDEKPKEVSSDSAQPVSEDASHKRVSSNSPKPASEDVKPAYWTQPHLVTGYCTYLPFRGFDITQTPPTYINVLPTLPQYTNIYTPLPNVSSEYQLQRSVPVVPSFIPSERLDESATVYFEGHHLNAENAPGNQIVPKTQILQDSMEVSVKTECSTDGADTALSESNRYDGHCGNSANKWEVNPEKNNEVTNTPHTQMVAIQVSWNITHQEVNTEPYDPFETQQGDISQIEKEFQVLQEQLNEACENYEQRKLKGSEETRDLEEKLKRNLEENKISKTELDWFLEDLEKEIKKWQQEKKEIQESLKALTKKMRKVLNANEIYAQKNDGKEKEHELLLDQSLEISNTFTNEKMKVEECIKKGKENYEESLQRAVAAEVSVLENWKDIEVYKLQIMESQAEGYLKNLRLLSSDSATYPDKESDIHSWESFLSKVKKEIEKAKSQYEEQIQAIKNGSRLSELSKVQVPELSFPVCSTILPTSLPESSGHEDHRASISTSHEAGNQTAVPKDSSLVSASDGPVGASSPPLTGSLSCQPEVTQLPECKSTGQETPPKQSLVADQKLPVLPGRATRSSQSPKKPFNSIIEHLSVVFPCYNSTELAGFIKKVRNKNKNSLSGLSIDEIVQRVTEHILDEQKKKKPNSNSAKDKRLSEPSSTASVNRASQGPPSVTAGPSPKTKGQKTEDAPASGAASCELCHEIFKSKNVRVLKCGHKFHKGCFKQWLKGQSTCPACLDQDLL

Target ORF information:

RefSeq Version	XM_005638907.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638907.3

ORF Insert Sequence:

ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA 
GAAGACCTGC AAGGTCGAAC ACCAACTAGA AATCCAATTA AAGCCTTTTA TGAAAGCAGG 
GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT 
GAAAAAGATT CCAGAAAAAC TAACCGCGAA ATGGCAAACG GTGGTAATCA GAATCTAAAG 
GTGGTAGATG AAGCTTTGAA GGTAGATGAT TGTGACTGTC ATCCTGAATT TCTACCACCA 
TCAGGTCAGA CTCCAAAATA CAAAGGAAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG 
TTCAGTTCTA GTTCACAGGG GAGTTTACCA GTAGATTTGA AGAACATTTT GGAAAAACAA 
TTCTCTAAAT CTTCCAGAGC TGCACATCAG GATTTTGCTA ATATAATGAA AATGCTGAGA 
AGCTTAATCC AGGATGGTTA CACGGCTTTG TTGGAGCAGC GTTGCCGCAG TGCTGCACAG 
GCCTTCACAG AATTGCTCAA TGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC 
ATGATTAACT ATGTCTTAGT AGTCTACGGA CTTGCTATTT CTCTCCTTGG AATAGGACAG 
CCTGAGGAAT TATCTGAAGC TGAAAACCAG TTTAAGAGGA TGATTGAACA CTACCCCAAT 
GAGGGACTTG ATTGTTTGGC CTACTGTGGA ATTGGAAAAG TATATTTGAA AAAGAACAGA 
TTTCTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATTTGTCG TCTTCCTGGA 
GTGTTAACTT GGCCCACAAG TAATGTGATT ATTGAAGAGA CTCAGCCGGG GAAAATAAAG 
ATGCTGTTAG AGAAGTTTAT TGAAGAATGC AGGTTCCCTC CAGTGCCAGA TGCCATTTGT 
TGCTATCAGA AGTGCCGTGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT 
AAGGGTTTTA TACGAATCAG CTGCTGCCAG TACTGTAAAA TAGAATTTCA CATGAACTGT 
TGGAAAAAGT TGAAAACAAC AACTTTTAAT GATAAAATTG ACAAGGATTT TCTACAAGGA 
ATTTGTCTTA CCCCTGACTG TGAAGGAATC ATTTCTAAGA TTATCATTTT CAGCAGTGGT 
GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATAAAAG AAAAGGTTCC TCCAAGACCT 
ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGCTAAGAC TGAAAGAAGA CAAAAAATTG 
AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA AAATTAGCAC AAGAAAGAAT GGAAGAGGAC 
CTAAGGGAAA GTAATCCACC CAAAACTGAA GAGCAGAAAG AAACTGTAGA CAGTGTTCAG 
AGTTGCCAGT TCCTTGACGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATA 
AAATCTGGTA TTCTGAACAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA 
AGCACAGAAG ACTACACAAC CTGTTTTTCT AGCAGAAATT TTCTAAATGA AGCGGTGGAC 
TACGTCATTC GTCACTTGAT TCAAGAAAAG AATAGAGTAA AGACTAGGAT ATTTCTGCAT 
GTTTTGAGCG AACTTAAAGA AGTGGAGCCA AAATTAGCCG CTTGGATCCA GAAACTTAAC 
AGCTTTGGTT TAGATGCCAC AGGACCTTTT TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG 
CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ATGGTCATAA ACTAGCCTCT 
ATAGAAGGAA AGCAACTTGA TTATTTCTGT GAGCCAACAT CAGTGAAGGA AGCACGGTGT 
TTAATATGGC TGCTAGAAGA GCATAGAGAC AAGTTCCCAG CGCTACACAA TGCTCTAGAT 
GAGTTCTTTG ACATAATGGA TAGCCGCTGT ACTGTATTAA GGAAACAAGA CAGTGGCGAT 
GTGCCGTTTA ATTCCGCCAA GATAAAAAAT AAAGGCAAGA AAAAGAAGCC AAAAGATTCA 
AAGCCTATGT TGGTGGGGTC TGGGACACCT TCTGTAACAC CAAATAATGA GACTATCACT 
TCGGGTGAAG AACATAACAG ACGCAATTCA AATTCTGCAG GGCCATTTGT GGTACCTGAC 
CACCTTCGGC AAGATGTAGA AGAATTTGAA GCTATCTATG AGCAGCACAG TAGTGAATAT 
GTTGTCCGTA ATAAAAAGCT GTGGGACATT AACCCAAAAC AAAAATGTTC AACTCTGTAT 
GATTATTTCT CTCAGTTGTT GGAGGAGCAT GGTCCCTTGG ACATGAGTAA TAAAATGTTC 
TCTGAAGAAT ATGAATTCTT CCCAGAAGAA ACTAGACAGA TACTAGAAAA AGCTGGAGGT 
TTGAAATCTT TCCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGACAACTG TATTGCTTTG 
AAGAAAGTTG CTTTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA GACAAAAGTG 
GAAGAAATTT CAAAAACAGG GGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT 
AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAATGTCTG ATTTATCTTC AGCAACAGCT 
TCTGAGAATG TGAAGCCCAA ACCTGTAGCT GCTAATTCTC CCAAATCAAC CTGTGAAGTT 
ATGAAGCCAA GACCAGTGTC TGATAATTCT TCTAGATCAG TTTCTGAAGA TGAGAAGCCC 
AAAGAAGTCT CTTCTGATTC TGCCCAACCT GTATCTGAGG ATGCAAGTCA CAAGCGAGTA 
TCCTCTAATT CTCCTAAACC AGCTTCTGAG GATGTGAAAC CAGCCTACTG GACTCAGCCC 
CATTTGGTCA CAGGATACTG TACATACCTC CCCTTTCGGG GATTTGACAT TACCCAGACA 
CCACCAACAT ACATAAATGT GTTACCAACT TTGCCCCAGT ACACTAACAT TTACACACCT 
CTGCCCAATG TTTCTTCTGA ATATCAGCTG CAAAGATCAG TGCCAGTGGT GCCGTCTTTT 
ATACCCAGTG AAAGACTAGA TGAAAGTGCT ACTGTGTACT TTGAGGGTCA TCATTTGAAT 
GCTGAGAATG CCCCTGGGAA CCAGATTGTC CCTAAAACAC AGATCCTTCA GGACTCTATG 
GAAGTGTCAG TAAAGACAGA GTGCAGCACA GATGGTGCTG ATACAGCCCT TAGTGAGTCT 
AACAGATATG ATGGTCACTG TGGAAATTCT GCCAACAAGT GGGAAGTAAA TCCAGAAAAA 
AACAATGAAG TAACAAATAC TCCACATACA CAAATGGTTG CCATACAGGT GTCTTGGAAT 
ATAACACATC AAGAAGTCAA TACTGAGCCA TATGATCCTT TTGAGACACA ACAGGGAGAT 
ATTTCACAGA TTGAGAAGGA GTTCCAGGTT TTACAAGAGC AGCTTAACGA GGCATGTGAA 
AATTATGAGC AGAGAAAACT CAAGGGCTCA GAAGAGACCA GGGATCTGGA AGAAAAATTG 
AAAAGGAACT TAGAAGAAAA CAAGATCTCA AAGACAGAAT TAGATTGGTT CCTTGAAGAC 
TTGGAAAAGG AAATCAAGAA ATGGCAACAG GAGAAAAAAG AAATCCAAGA AAGTCTGAAA 
GCGCTAACAA AGAAAATGAG AAAGGTTTTA AATGCCAACG AGATATATGC CCAGAAAAAT 
GATGGAAAGG AAAAGGAACA TGAATTACTT CTGGATCAGT CCCTTGAAAT CAGTAATACG 
TTTACAAATG AGAAAATGAA AGTAGAAGAA TGTATAAAGA AAGGGAAGGA GAATTATGAA 
GAGAGTCTTC AAAGAGCTGT GGCTGCAGAG GTATCTGTGC TTGAAAACTG GAAGGACATT 
GAAGTGTACA AATTACAGAT CATGGAATCA CAAGCAGAAG GGTATCTTAA GAACCTGAGG 
CTACTGAGCA GTGATTCTGC AACTTACCCT GATAAGGAAT CTGATATACA TTCATGGGAA 
TCATTTCTTT CTAAGGTTAA AAAAGAAATT GAAAAAGCAA AGTCTCAGTA TGAAGAACAA 
ATTCAAGCAA TTAAAAATGG CTCTCGACTC AGTGAACTTT CTAAAGTGCA AGTTCCTGAG 
CTTTCATTTC CTGTCTGTAG CACAATTCTT CCTACATCAC TTCCTGAGTC TTCAGGCCAT 
GAAGATCACA GAGCCTCCAT TTCTACAAGT CATGAGGCTG GAAACCAAAC AGCAGTTCCT 
AAGGACTCAA GTCTGGTCTC TGCCAGTGAC GGCCCAGTGG GGGCTTCCTC TCCACCATTG 
ACAGGATCGC TCTCCTGTCA GCCCGAAGTC ACCCAGCTGC CAGAGTGTAA GAGCACAGGC 
CAGGAAACTC CGCCAAAACA AAGCCTTGTG GCTGATCAGA AACTACCTGT TCTTCCAGGA 
CGTGCTACCC GCTCAAGCCA GTCTCCAAAA AAGCCGTTCA ACAGTATTAT TGAGCACCTG 
TCAGTGGTAT TCCCATGTTA CAACAGCACT GAGCTTGCTG GTTTTATTAA AAAAGTGCGA 
AACAAGAACA AAAACTCGCT CTCAGGATTG AGTATTGATG AAATTGTCCA AAGAGTGACA 
GAACACATTC TAGATGAACA GAAAAAGAAA AAGCCAAATT CAAATTCAGC AAAGGACAAG 
AGGTTGTCTG AGCCCAGCTC CACTGCTTCT GTGAACAGGG CCTCCCAGGG TCCACCCTCC 
GTGACTGCTG GACCATCGCC CAAAACCAAG GGACAAAAGA CAGAAGATGC CCCTGCATCG 
GGTGCAGCTT CCTGTGAACT GTGCCATGAG ATATTCAAAT CCAAAAACGT GCGTGTACTA 
AAATGTGGGC ACAAGTTTCA CAAAGGGTGC TTTAAGCAGT GGCTGAAGGG GCAGAGCACT 
TGCCCGGCCT GCCTCGATCA GGATCTCCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005638905.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638962.1 Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe29424
Clone ID Related Accession (Same CDS sequence)	XM_005638905.2 , XM_014109729.1 , XM_005638905.3
Accession Version	XM_005638905.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638905.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATT TTGCCCTGGG AGATTTCACT ATAGCAGATG ATGCTTTGTC AGAAGATTGC 
CCTTATGTGG ACGGTTGTGT CTTTGCTCCT GAATTTATGT CCAATGATTA TGTTCGTGTG 
ACTCAACTTT ACTGCGATGG GGTGGGTATG CAGTATAAAG ATTTTACCCA AAGTGAGAGA 
AACTTAGAAT TTGACATCTG TAATATGTGG TGTAGTAAAC CAGTTTCTGT CCTGCAAGAT 
TATTGTGATG CCATTAAAAT ATACATCTTC TGGCCACTTC TGTTTCAACG TCAGCATAGT 
TCTATAATAT CACGGTTACA CCCCTGTGTG GAGGCCACCA ACTCTCATGC TTCTGAGATA 
AGTTTGAAGA AATTGCAACA TCTAGAATTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTCGTGAATG ATTCATTCTT TATTGGGGGC TTATTGAGGA TTGGTTATAA AATAGAAAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT ATACAGGCGA TGTAACAATT 
CTACCTAAAT TAGGAGCAGT TGACAATTGT TGGCCTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AGTTGTAACA GAAAACTGCA ATTTGCTTGA AGAATTTAAA 
ACTCAAAATT GTGCGGAATG TATAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCAA AAGAAAGATT TGATATAGCT ATTATCTATT ACACCAGAGC CATTGAATAT 
AGACCTGAAA ATCACCTTCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG CACTGGACAG 
TTTAGAAATG CACTTGGTGA TGGAAAGAGA GCCACTATCC TGAAGAGCAA TTGGACGAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCGAAC ACCAACTAGA 
AATCCAATTA AAGCCTTTTA TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAGATT CCAGAAAAAC TAACCGCGAA 
ATGGCAAACG GTGGTAATCA GAATCTAAAG GTGGTAGATG AAGCTTTGAA GGTAGATGAT 
TGTGACTGTC ATCCTGAATT TCTACCACCA TCAGGTCAGA CTCCAAAATA CAAAGGAAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGTTCTA GTTCACAGGG GAGTTTACCA 
GTAGATTTGA AGAACATTTT GGAAAAACAA TTCTCTAAAT CTTCCAGAGC TGCACATCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGTTA CACGGCTTTG 
TTGGAGCAGC GTTGCCGCAG TGCTGCACAG GCCTTCACAG AATTGCTCAA TGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAACCTGGCC ATGATTAACT ATGTCTTAGT AGTCTACGGA 
CTTGCTATTT CTCTCCTTGG AATAGGACAG CCTGAGGAAT TATCTGAAGC TGAAAACCAG 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGTTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAGAACAGA TTTCTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATTTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGATT 
ATTGAAGAGA CTCAGCCGGG GAAAATAAAG ATGCTGTTAG AGAAGTTTAT TGAAGAATGC 
AGGTTCCCTC CAGTGCCAGA TGCCATTTGT TGCTATCAGA AGTGCCGTGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGAATCAG CTGCTGCCAG 
TACTGTAAAA TAGAATTTCA CATGAACTGT TGGAAAAAGT TGAAAACAAC AACTTTTAAT 
GATAAAATTG ACAAGGATTT TCTACAAGGA ATTTGTCTTA CCCCTGACTG TGAAGGAATC 
ATTTCTAAGA TTATCATTTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATAAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGCTAAGAC TGAAAGAAGA CAAAAAATTG AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA 
AAATTAGCAC AAGAAAGAAT GGAAGAGGAC CTAAGGGAAA GTAATCCACC CAAAACTGAA 
GAGCAGAAAG AAACTGTAGA CAGTGTTCAG AGTTGCCAGT TCCTTGACGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATA AAATCTGGTA TTCTGAACAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA AGCACAGAAG ACTACACAAC CTGTTTTTCT 
AGCAGAAATT TTCTAAATGA AGCGGTGGAC TACGTCATTC GTCACTTGAT TCAAGAAAAG 
AATAGAGTAA AGACTAGGAT ATTTCTGCAT GTTTTGAGCG AACTTAAAGA AGTGGAGCCA 
AAATTAGCCG CTTGGATCCA GAAACTTAAC AGCTTTGGTT TAGATGCCAC AGGACCTTTT 
TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA ACTAGCCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTGT 
GAGCCAACAT CAGTGAAGGA AGCACGGTGT TTAATATGGC TGCTAGAAGA GCATAGAGAC 
AAGTTCCCAG CGCTACACAA TGCTCTAGAT GAGTTCTTTG ACATAATGGA TAGCCGCTGT 
ACTGTATTAA GGAAACAAGA CAGTGGCGAT GTGCCGTTTA ATTCCGCCAA GATAAAAAAT 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TGGTGGGGTC TGGGACACCT 
TCTGTAACAC CAAATAATGA GACTATCACT TCGGGTGAAG AACATAACAG ACGCAATTCA 
AATTCTGCAG GGCCATTTGT GGTACCTGAC CACCTTCGGC AAGATGTAGA AGAATTTGAA 
GCTATCTATG AGCAGCACAG TAGTGAATAT GTTGTCCGTA ATAAAAAGCT GTGGGACATT 
AACCCAAAAC AAAAATGTTC AACTCTGTAT GATTATTTCT CTCAGTTGTT GGAGGAGCAT 
GGTCCCTTGG ACATGAGTAA TAAAATGTTC TCTGAAGAAT ATGAATTCTT CCCAGAAGAA 
ACTAGACAGA TACTAGAAAA AGCTGGAGGT TTGAAATCTT TCCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCTTTG AAGAAAGTTG CTTTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTG GAAGAAATTT CAAAAACAGG GGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAATGTCTG ATTTATCTTC AGCAACAGCT TCTGAGAATG TGAAGCCCAA ACCTGTAGCT 
GCTAATTCTC CCAAATCAAC CTGTGAAGTT ATGAAGCCAA GACCAGTGTC TGATAATTCT 
TCTAGATCAG TTTCTGAAGA TGAGAAGCCC AAAGAAGTCT CTTCTGATTC TGCCCAACCT 
GTATCTGAGG ATGCAAGTCA CAAGCGAGTA TCCTCTAATT CTCCTAAACC AGCTTCTGAG 
GATGTGAAAC CAGCCTACTG GACTCAGCCC CATTTGGTCA CAGGATACTG TACATACCTC 
CCCTTTCGGG GATTTGACAT TACCCAGACA CCACCAACAT ACATAAATGT GTTACCAACT 
TTGCCCCAGT ACACTAACAT TTACACACCT CTGCCCAATG TTTCTTCTGA ATATCAGCTG 
CAAAGATCAG TGCCAGTGGT GCCGTCTTTT ATACCCAGTG AAAGACTAGA TGAAAGTGCT 
ACTGTGTACT TTGAGGGTCA TCATTTGAAT GCTGAGAATG CCCCTGGGAA CCAGATTGTC 
CCTAAAACAC AGATCCTTCA GGACTCTATG GAAGTGTCAG TAAAGACAGA GTGCAGCACA 
GATGGTGCTG ATACAGCCCT TAGTGAGTCT AACAGATATG ATGGTCACTG TGGAAATTCT 
GCCAACAAGT GGGAAGTAAA TCCAGAAAAA AACAATGAAG TAACAAATAC TCCACATACA 
CAAATGGTTG CCATACAGGT GTCTTGGAAT ATAACACATC AAGAAGTCAA TACTGAGCCA 
TATGATCCTT TTGAGACACA ACAGGGAGAT ATTTCACAGA TTGAGAAGGA GTTCCAGGTT 
TTACAAGAGC AGCTTAACGA GGCATGTGAA AATTATGAGC AGAGAAAACT CAAGGGCTCA 
GAAGAGACCA GGGATCTGGA AGAAAAATTG AAAAGGAACT TAGAAGAAAA CAAGATCTCA 
AAGACAGAAT TAGATTGGTT CCTTGAAGAC TTGGAAAAGG AAATCAAGAA ATGGCAACAG 
GAGAAAAAAG AAATCCAAGA AAGTCTGAAA GCGCTAACAA AGAAAATGAG AAAGGTTTTA 
AATGCCAACG AGATATATGC CCAGAAAAAT GATGGAAAGG AAAAGGAACA TGAATTACTT 
CTGGATCAGT CCCTTGAAAT CAGTAATACG TTTACAAATG AGAAAATGAA AGTAGAAGAA 
TGTATAAAGA AAGGGAAGGA GAATTATGAA GAGAGTCTTC AAAGAGCTGT GGCTGCAGAG 
GTATCTGTGC TTGAAAACTG GAAGGACATT GAAGTGTACA AATTACAGAT CATGGAATCA 
CAAGCAGAAG GGTATCTTAA GAACCTGAGG CTACTGAGCA GTGATTCTGC AACTTACCCT 
GATAAGGAAT CTGATATACA TTCATGGGAA TCATTTCTTT CTAAGGTTAA AAAAGAAATT 
GAAAAAGCAA AGTCTCAGTA TGAAGAACAA ATTCAAGCAA TTAAAAATGG CTCTCGACTC 
AGTGAACTTT CTAAAGTGCA AGTTCCTGAG CTTTCATTTC CTGTCTGTAG CACAATTCTT 
CCTACATCAC TTCCTGAGTC TTCAGGCCAT GAAGATCACA GAGCCTCCAT TTCTACAAGT 
CATGAGGCTG GAAACCAAAC AGCAGTTCCT AAGGACTCAA GTCTGGTCTC TGCCAGTGAC 
GGCCCAGTGG GGGCTTCCTC TCCACCATTG ACAGGATCGC TCTCCTGTCA GCCCGAAGTC 
ACCCAGCTGC CAGAGTGTAA GAGCACAGGC CAGGAAACTC CGCCAAAACA AAGCCTTGTG 
GCTGATCAGA AACTACCTGT TCTTCCAGGA CGTGCTACCC GCTCAAGCCA GTCTCCAAAA 
AAGCCGTTCA ACAGTATTAT TGAGCACCTG TCAGTGGTAT TCCCATGTTA CAACAGCACT 
GAGCTTGCTG GTTTTATTAA AAAAGTGCGA AACAAGAACA AAAACTCGCT CTCAGGATTG 
AGTATTGATG AAATTGTCCA AAGAGTGACA GAACACATTC TAGATGAACA GAAAAAGAAA 
AAGCCAAATT CAAATTCAGC AAAGGACAAG AGGTTGTCTG AGCCCAGCTC CACTGCTTCT 
GTGAACAGGG CCTCCCAGGG TCCACCCTCC GTGACTGCTG GACCATCGCC CAAAACCAAG 
GGACAAAAGA CAGAAGATGC CCCTGCATCG GGTGCAGCTT CCTGTGAACT GTGCCATGAG 
ATATTCAAAT CCAAAAACGT GCGTGTACTA AAATGTGGGC ACAAGTTTCA CAAAGGGTGC 
TTTAAGCAGT GGCTGAAGGG GCAGAGCACT TGCCCGGCCT GCCTCGATCA GGATCTCCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638962.1
CDS	1586..7588
Translation	MDDFALGDFTIADDALSEDCPYVDGCVFAPEFMSNDYVRVTQLYCDGVGMQYKDFTQSERNLEFDICNMWCSKPVSVLQDYCDAIKIYIFWPLLFQRQHSSIISRLHPCVEATNSHASEISLKKLQHLELMEDIVDLAKKVVNDSFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILPKLGAVDNCWPMLSIFFTEYKYHITKVVTENCNLLEEFKTQNCAECIEQGELMKMKGNEEFSKERFDIAIIYYTRAIEYRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKSNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPTRNPIKAFYESRAYIPSLSAPAFSTSLNFVETEKDSRKTNREMANGGNQNLKVVDEALKVDDCDCHPEFLPPSGQTPKYKGKQKSRNNELEKFSSSSQGSLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFIEECRFPPVPDAICCYQKCRGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKIQKKEAKKLAQERMEEDLRESNPPKTEEQKETVDSVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLSTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRIFLHVLSELKEVEPKLAAWIQKLNSFGLDATGPFFSRYGASLKELDFSIMTFLWNEKYGHKLASIEGKQLDYFCEPTSVKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSAKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETITSGEEHNRRNSNSAGPFVVPDHLRQDVEEFEAIYEQHSSEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKSFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPMSDLSSATASENVKPKPVAANSPKSTCEVMKPRPVSDNSSRSVSEDEKPKEVSSDSAQPVSEDASHKRVSSNSPKPASEDVKPAYWTQPHLVTGYCTYLPFRGFDITQTPPTYINVLPTLPQYTNIYTPLPNVSSEYQLQRSVPVVPSFIPSERLDESATVYFEGHHLNAENAPGNQIVPKTQILQDSMEVSVKTECSTDGADTALSESNRYDGHCGNSANKWEVNPEKNNEVTNTPHTQMVAIQVSWNITHQEVNTEPYDPFETQQGDISQIEKEFQVLQEQLNEACENYEQRKLKGSEETRDLEEKLKRNLEENKISKTELDWFLEDLEKEIKKWQQEKKEIQESLKALTKKMRKVLNANEIYAQKNDGKEKEHELLLDQSLEISNTFTNEKMKVEECIKKGKENYEESLQRAVAAEVSVLENWKDIEVYKLQIMESQAEGYLKNLRLLSSDSATYPDKESDIHSWESFLSKVKKEIEKAKSQYEEQIQAIKNGSRLSELSKVQVPELSFPVCSTILPTSLPESSGHEDHRASISTSHEAGNQTAVPKDSSLVSASDGPVGASSPPLTGSLSCQPEVTQLPECKSTGQETPPKQSLVADQKLPVLPGRATRSSQSPKKPFNSIIEHLSVVFPCYNSTELAGFIKKVRNKNKNSLSGLSIDEIVQRVTEHILDEQKKKKPNSNSAKDKRLSEPSSTASVNRASQGPPSVTAGPSPKTKGQKTEDAPASGAASCELCHEIFKSKNVRVLKCGHKFHKGCFKQWLKGQSTCPACLDQDLL

Target ORF information:

RefSeq Version	XM_005638905.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638905.2

ORF Insert Sequence:

ATGGATGATT TTGCCCTGGG AGATTTCACT ATAGCAGATG ATGCTTTGTC AGAAGATTGC 
CCTTATGTGG ACGGTTGTGT CTTTGCTCCT GAATTTATGT CCAATGATTA TGTTCGTGTG 
ACTCAACTTT ACTGCGATGG GGTGGGTATG CAGTATAAAG ATTTTACCCA AAGTGAGAGA 
AACTTAGAAT TTGACATCTG TAATATGTGG TGTAGTAAAC CAGTTTCTGT CCTGCAAGAT 
TATTGTGATG CCATTAAAAT ATACATCTTC TGGCCACTTC TGTTTCAACG TCAGCATAGT 
TCTATAATAT CACGGTTACA CCCCTGTGTG GAGGCCACCA ACTCTCATGC TTCTGAGATA 
AGTTTGAAGA AATTGCAACA TCTAGAATTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTCGTGAATG ATTCATTCTT TATTGGGGGC TTATTGAGGA TTGGTTATAA AATAGAAAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT ATACAGGCGA TGTAACAATT 
CTACCTAAAT TAGGAGCAGT TGACAATTGT TGGCCTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AGTTGTAACA GAAAACTGCA ATTTGCTTGA AGAATTTAAA 
ACTCAAAATT GTGCGGAATG TATAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCAA AAGAAAGATT TGATATAGCT ATTATCTATT ACACCAGAGC CATTGAATAT 
AGACCTGAAA ATCACCTTCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG CACTGGACAG 
TTTAGAAATG CACTTGGTGA TGGAAAGAGA GCCACTATCC TGAAGAGCAA TTGGACGAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCGAAC ACCAACTAGA 
AATCCAATTA AAGCCTTTTA TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAGATT CCAGAAAAAC TAACCGCGAA 
ATGGCAAACG GTGGTAATCA GAATCTAAAG GTGGTAGATG AAGCTTTGAA GGTAGATGAT 
TGTGACTGTC ATCCTGAATT TCTACCACCA TCAGGTCAGA CTCCAAAATA CAAAGGAAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGTTCTA GTTCACAGGG GAGTTTACCA 
GTAGATTTGA AGAACATTTT GGAAAAACAA TTCTCTAAAT CTTCCAGAGC TGCACATCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGTTA CACGGCTTTG 
TTGGAGCAGC GTTGCCGCAG TGCTGCACAG GCCTTCACAG AATTGCTCAA TGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAACCTGGCC ATGATTAACT ATGTCTTAGT AGTCTACGGA 
CTTGCTATTT CTCTCCTTGG AATAGGACAG CCTGAGGAAT TATCTGAAGC TGAAAACCAG 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGTTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAGAACAGA TTTCTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATTTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGATT 
ATTGAAGAGA CTCAGCCGGG GAAAATAAAG ATGCTGTTAG AGAAGTTTAT TGAAGAATGC 
AGGTTCCCTC CAGTGCCAGA TGCCATTTGT TGCTATCAGA AGTGCCGTGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGAATCAG CTGCTGCCAG 
TACTGTAAAA TAGAATTTCA CATGAACTGT TGGAAAAAGT TGAAAACAAC AACTTTTAAT 
GATAAAATTG ACAAGGATTT TCTACAAGGA ATTTGTCTTA CCCCTGACTG TGAAGGAATC 
ATTTCTAAGA TTATCATTTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATAAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGCTAAGAC TGAAAGAAGA CAAAAAATTG AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA 
AAATTAGCAC AAGAAAGAAT GGAAGAGGAC CTAAGGGAAA GTAATCCACC CAAAACTGAA 
GAGCAGAAAG AAACTGTAGA CAGTGTTCAG AGTTGCCAGT TCCTTGACGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATA AAATCTGGTA TTCTGAACAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA AGCACAGAAG ACTACACAAC CTGTTTTTCT 
AGCAGAAATT TTCTAAATGA AGCGGTGGAC TACGTCATTC GTCACTTGAT TCAAGAAAAG 
AATAGAGTAA AGACTAGGAT ATTTCTGCAT GTTTTGAGCG AACTTAAAGA AGTGGAGCCA 
AAATTAGCCG CTTGGATCCA GAAACTTAAC AGCTTTGGTT TAGATGCCAC AGGACCTTTT 
TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA ACTAGCCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTGT 
GAGCCAACAT CAGTGAAGGA AGCACGGTGT TTAATATGGC TGCTAGAAGA GCATAGAGAC 
AAGTTCCCAG CGCTACACAA TGCTCTAGAT GAGTTCTTTG ACATAATGGA TAGCCGCTGT 
ACTGTATTAA GGAAACAAGA CAGTGGCGAT GTGCCGTTTA ATTCCGCCAA GATAAAAAAT 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TGGTGGGGTC TGGGACACCT 
TCTGTAACAC CAAATAATGA GACTATCACT TCGGGTGAAG AACATAACAG ACGCAATTCA 
AATTCTGCAG GGCCATTTGT GGTACCTGAC CACCTTCGGC AAGATGTAGA AGAATTTGAA 
GCTATCTATG AGCAGCACAG TAGTGAATAT GTTGTCCGTA ATAAAAAGCT GTGGGACATT 
AACCCAAAAC AAAAATGTTC AACTCTGTAT GATTATTTCT CTCAGTTGTT GGAGGAGCAT 
GGTCCCTTGG ACATGAGTAA TAAAATGTTC TCTGAAGAAT ATGAATTCTT CCCAGAAGAA 
ACTAGACAGA TACTAGAAAA AGCTGGAGGT TTGAAATCTT TCCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCTTTG AAGAAAGTTG CTTTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTG GAAGAAATTT CAAAAACAGG GGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAATGTCTG ATTTATCTTC AGCAACAGCT TCTGAGAATG TGAAGCCCAA ACCTGTAGCT 
GCTAATTCTC CCAAATCAAC CTGTGAAGTT ATGAAGCCAA GACCAGTGTC TGATAATTCT 
TCTAGATCAG TTTCTGAAGA TGAGAAGCCC AAAGAAGTCT CTTCTGATTC TGCCCAACCT 
GTATCTGAGG ATGCAAGTCA CAAGCGAGTA TCCTCTAATT CTCCTAAACC AGCTTCTGAG 
GATGTGAAAC CAGCCTACTG GACTCAGCCC CATTTGGTCA CAGGATACTG TACATACCTC 
CCCTTTCGGG GATTTGACAT TACCCAGACA CCACCAACAT ACATAAATGT GTTACCAACT 
TTGCCCCAGT ACACTAACAT TTACACACCT CTGCCCAATG TTTCTTCTGA ATATCAGCTG 
CAAAGATCAG TGCCAGTGGT GCCGTCTTTT ATACCCAGTG AAAGACTAGA TGAAAGTGCT 
ACTGTGTACT TTGAGGGTCA TCATTTGAAT GCTGAGAATG CCCCTGGGAA CCAGATTGTC 
CCTAAAACAC AGATCCTTCA GGACTCTATG GAAGTGTCAG TAAAGACAGA GTGCAGCACA 
GATGGTGCTG ATACAGCCCT TAGTGAGTCT AACAGATATG ATGGTCACTG TGGAAATTCT 
GCCAACAAGT GGGAAGTAAA TCCAGAAAAA AACAATGAAG TAACAAATAC TCCACATACA 
CAAATGGTTG CCATACAGGT GTCTTGGAAT ATAACACATC AAGAAGTCAA TACTGAGCCA 
TATGATCCTT TTGAGACACA ACAGGGAGAT ATTTCACAGA TTGAGAAGGA GTTCCAGGTT 
TTACAAGAGC AGCTTAACGA GGCATGTGAA AATTATGAGC AGAGAAAACT CAAGGGCTCA 
GAAGAGACCA GGGATCTGGA AGAAAAATTG AAAAGGAACT TAGAAGAAAA CAAGATCTCA 
AAGACAGAAT TAGATTGGTT CCTTGAAGAC TTGGAAAAGG AAATCAAGAA ATGGCAACAG 
GAGAAAAAAG AAATCCAAGA AAGTCTGAAA GCGCTAACAA AGAAAATGAG AAAGGTTTTA 
AATGCCAACG AGATATATGC CCAGAAAAAT GATGGAAAGG AAAAGGAACA TGAATTACTT 
CTGGATCAGT CCCTTGAAAT CAGTAATACG TTTACAAATG AGAAAATGAA AGTAGAAGAA 
TGTATAAAGA AAGGGAAGGA GAATTATGAA GAGAGTCTTC AAAGAGCTGT GGCTGCAGAG 
GTATCTGTGC TTGAAAACTG GAAGGACATT GAAGTGTACA AATTACAGAT CATGGAATCA 
CAAGCAGAAG GGTATCTTAA GAACCTGAGG CTACTGAGCA GTGATTCTGC AACTTACCCT 
GATAAGGAAT CTGATATACA TTCATGGGAA TCATTTCTTT CTAAGGTTAA AAAAGAAATT 
GAAAAAGCAA AGTCTCAGTA TGAAGAACAA ATTCAAGCAA TTAAAAATGG CTCTCGACTC 
AGTGAACTTT CTAAAGTGCA AGTTCCTGAG CTTTCATTTC CTGTCTGTAG CACAATTCTT 
CCTACATCAC TTCCTGAGTC TTCAGGCCAT GAAGATCACA GAGCCTCCAT TTCTACAAGT 
CATGAGGCTG GAAACCAAAC AGCAGTTCCT AAGGACTCAA GTCTGGTCTC TGCCAGTGAC 
GGCCCAGTGG GGGCTTCCTC TCCACCATTG ACAGGATCGC TCTCCTGTCA GCCCGAAGTC 
ACCCAGCTGC CAGAGTGTAA GAGCACAGGC CAGGAAACTC CGCCAAAACA AAGCCTTGTG 
GCTGATCAGA AACTACCTGT TCTTCCAGGA CGTGCTACCC GCTCAAGCCA GTCTCCAAAA 
AAGCCGTTCA ACAGTATTAT TGAGCACCTG TCAGTGGTAT TCCCATGTTA CAACAGCACT 
GAGCTTGCTG GTTTTATTAA AAAAGTGCGA AACAAGAACA AAAACTCGCT CTCAGGATTG 
AGTATTGATG AAATTGTCCA AAGAGTGACA GAACACATTC TAGATGAACA GAAAAAGAAA 
AAGCCAAATT CAAATTCAGC AAAGGACAAG AGGTTGTCTG AGCCCAGCTC CACTGCTTCT 
GTGAACAGGG CCTCCCAGGG TCCACCCTCC GTGACTGCTG GACCATCGCC CAAAACCAAG 
GGACAAAAGA CAGAAGATGC CCCTGCATCG GGTGCAGCTT CCTGTGAACT GTGCCATGAG 
ATATTCAAAT CCAAAAACGT GCGTGTACTA AAATGTGGGC ACAAGTTTCA CAAAGGGTGC 
TTTAAGCAGT GGCTGAAGGG GCAGAGCACT TGCCCGGCCT GCCTCGATCA GGATCTCCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_014109729.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965204.1 Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

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CloneID	OCe29424
Clone ID Related Accession (Same CDS sequence)	XM_005638905.2 , XM_014109729.1 , XM_005638905.3
Accession Version	XM_014109729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATT TTGCCCTGGG AGATTTCACT ATAGCAGATG ATGCTTTGTC AGAAGATTGC 
CCTTATGTGG ACGGTTGTGT CTTTGCTCCT GAATTTATGT CCAATGATTA TGTTCGTGTG 
ACTCAACTTT ACTGCGATGG GGTGGGTATG CAGTATAAAG ATTTTACCCA AAGTGAGAGA 
AACTTAGAAT TTGACATCTG TAATATGTGG TGTAGTAAAC CAGTTTCTGT CCTGCAAGAT 
TATTGTGATG CCATTAAAAT ATACATCTTC TGGCCACTTC TGTTTCAACG TCAGCATAGT 
TCTATAATAT CACGGTTACA CCCCTGTGTG GAGGCCACCA ACTCTCATGC TTCTGAGATA 
AGTTTGAAGA AATTGCAACA TCTAGAATTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTCGTGAATG ATTCATTCTT TATTGGGGGC TTATTGAGGA TTGGTTATAA AATAGAAAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT ATACAGGCGA TGTAACAATT 
CTACCTAAAT TAGGAGCAGT TGACAATTGT TGGCCTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AGTTGTAACA GAAAACTGCA ATTTGCTTGA AGAATTTAAA 
ACTCAAAATT GTGCGGAATG TATAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCAA AAGAAAGATT TGATATAGCT ATTATCTATT ACACCAGAGC CATTGAATAT 
AGACCTGAAA ATCACCTTCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG CACTGGACAG 
TTTAGAAATG CACTTGGTGA TGGAAAGAGA GCCACTATCC TGAAGAGCAA TTGGACGAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCGAAC ACCAACTAGA 
AATCCAATTA AAGCCTTTTA TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAGATT CCAGAAAAAC TAACCGCGAA 
ATGGCAAACG GTGGTAATCA GAATCTAAAG GTGGTAGATG AAGCTTTGAA GGTAGATGAT 
TGTGACTGTC ATCCTGAATT TCTACCACCA TCAGGTCAGA CTCCAAAATA CAAAGGAAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGTTCTA GTTCACAGGG GAGTTTACCA 
GTAGATTTGA AGAACATTTT GGAAAAACAA TTCTCTAAAT CTTCCAGAGC TGCACATCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGTTA CACGGCTTTG 
TTGGAGCAGC GTTGCCGCAG TGCTGCACAG GCCTTCACAG AATTGCTCAA TGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAACCTGGCC ATGATTAACT ATGTCTTAGT AGTCTACGGA 
CTTGCTATTT CTCTCCTTGG AATAGGACAG CCTGAGGAAT TATCTGAAGC TGAAAACCAG 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGTTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAGAACAGA TTTCTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATTTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGATT 
ATTGAAGAGA CTCAGCCGGG GAAAATAAAG ATGCTGTTAG AGAAGTTTAT TGAAGAATGC 
AGGTTCCCTC CAGTGCCAGA TGCCATTTGT TGCTATCAGA AGTGCCGTGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGAATCAG CTGCTGCCAG 
TACTGTAAAA TAGAATTTCA CATGAACTGT TGGAAAAAGT TGAAAACAAC AACTTTTAAT 
GATAAAATTG ACAAGGATTT TCTACAAGGA ATTTGTCTTA CCCCTGACTG TGAAGGAATC 
ATTTCTAAGA TTATCATTTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATAAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGCTAAGAC TGAAAGAAGA CAAAAAATTG AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA 
AAATTAGCAC AAGAAAGAAT GGAAGAGGAC CTAAGGGAAA GTAATCCACC CAAAACTGAA 
GAGCAGAAAG AAACTGTAGA CAGTGTTCAG AGTTGCCAGT TCCTTGACGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATA AAATCTGGTA TTCTGAACAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA AGCACAGAAG ACTACACAAC CTGTTTTTCT 
AGCAGAAATT TTCTAAATGA AGCGGTGGAC TACGTCATTC GTCACTTGAT TCAAGAAAAG 
AATAGAGTAA AGACTAGGAT ATTTCTGCAT GTTTTGAGCG AACTTAAAGA AGTGGAGCCA 
AAATTAGCCG CTTGGATCCA GAAACTTAAC AGCTTTGGTT TAGATGCCAC AGGACCTTTT 
TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA ACTAGCCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTGT 
GAGCCAACAT CAGTGAAGGA AGCACGGTGT TTAATATGGC TGCTAGAAGA GCATAGAGAC 
AAGTTCCCAG CGCTACACAA TGCTCTAGAT GAGTTCTTTG ACATAATGGA TAGCCGCTGT 
ACTGTATTAA GGAAACAAGA CAGTGGCGAT GTGCCGTTTA ATTCCGCCAA GATAAAAAAT 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TGGTGGGGTC TGGGACACCT 
TCTGTAACAC CAAATAATGA GACTATCACT TCGGGTGAAG AACATAACAG ACGCAATTCA 
AATTCTGCAG GGCCATTTGT GGTACCTGAC CACCTTCGGC AAGATGTAGA AGAATTTGAA 
GCTATCTATG AGCAGCACAG TAGTGAATAT GTTGTCCGTA ATAAAAAGCT GTGGGACATT 
AACCCAAAAC AAAAATGTTC AACTCTGTAT GATTATTTCT CTCAGTTGTT GGAGGAGCAT 
GGTCCCTTGG ACATGAGTAA TAAAATGTTC TCTGAAGAAT ATGAATTCTT CCCAGAAGAA 
ACTAGACAGA TACTAGAAAA AGCTGGAGGT TTGAAATCTT TCCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCTTTG AAGAAAGTTG CTTTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTG GAAGAAATTT CAAAAACAGG GGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAATGTCTG ATTTATCTTC AGCAACAGCT TCTGAGAATG TGAAGCCCAA ACCTGTAGCT 
GCTAATTCTC CCAAATCAAC CTGTGAAGTT ATGAAGCCAA GACCAGTGTC TGATAATTCT 
TCTAGATCAG TTTCTGAAGA TGAGAAGCCC AAAGAAGTCT CTTCTGATTC TGCCCAACCT 
GTATCTGAGG ATGCAAGTCA CAAGCGAGTA TCCTCTAATT CTCCTAAACC AGCTTCTGAG 
GATGTGAAAC CAGCCTACTG GACTCAGCCC CATTTGGTCA CAGGATACTG TACATACCTC 
CCCTTTCGGG GATTTGACAT TACCCAGACA CCACCAACAT ACATAAATGT GTTACCAACT 
TTGCCCCAGT ACACTAACAT TTACACACCT CTGCCCAATG TTTCTTCTGA ATATCAGCTG 
CAAAGATCAG TGCCAGTGGT GCCGTCTTTT ATACCCAGTG AAAGACTAGA TGAAAGTGCT 
ACTGTGTACT TTGAGGGTCA TCATTTGAAT GCTGAGAATG CCCCTGGGAA CCAGATTGTC 
CCTAAAACAC AGATCCTTCA GGACTCTATG GAAGTGTCAG TAAAGACAGA GTGCAGCACA 
GATGGTGCTG ATACAGCCCT TAGTGAGTCT AACAGATATG ATGGTCACTG TGGAAATTCT 
GCCAACAAGT GGGAAGTAAA TCCAGAAAAA AACAATGAAG TAACAAATAC TCCACATACA 
CAAATGGTTG CCATACAGGT GTCTTGGAAT ATAACACATC AAGAAGTCAA TACTGAGCCA 
TATGATCCTT TTGAGACACA ACAGGGAGAT ATTTCACAGA TTGAGAAGGA GTTCCAGGTT 
TTACAAGAGC AGCTTAACGA GGCATGTGAA AATTATGAGC AGAGAAAACT CAAGGGCTCA 
GAAGAGACCA GGGATCTGGA AGAAAAATTG AAAAGGAACT TAGAAGAAAA CAAGATCTCA 
AAGACAGAAT TAGATTGGTT CCTTGAAGAC TTGGAAAAGG AAATCAAGAA ATGGCAACAG 
GAGAAAAAAG AAATCCAAGA AAGTCTGAAA GCGCTAACAA AGAAAATGAG AAAGGTTTTA 
AATGCCAACG AGATATATGC CCAGAAAAAT GATGGAAAGG AAAAGGAACA TGAATTACTT 
CTGGATCAGT CCCTTGAAAT CAGTAATACG TTTACAAATG AGAAAATGAA AGTAGAAGAA 
TGTATAAAGA AAGGGAAGGA GAATTATGAA GAGAGTCTTC AAAGAGCTGT GGCTGCAGAG 
GTATCTGTGC TTGAAAACTG GAAGGACATT GAAGTGTACA AATTACAGAT CATGGAATCA 
CAAGCAGAAG GGTATCTTAA GAACCTGAGG CTACTGAGCA GTGATTCTGC AACTTACCCT 
GATAAGGAAT CTGATATACA TTCATGGGAA TCATTTCTTT CTAAGGTTAA AAAAGAAATT 
GAAAAAGCAA AGTCTCAGTA TGAAGAACAA ATTCAAGCAA TTAAAAATGG CTCTCGACTC 
AGTGAACTTT CTAAAGTGCA AGTTCCTGAG CTTTCATTTC CTGTCTGTAG CACAATTCTT 
CCTACATCAC TTCCTGAGTC TTCAGGCCAT GAAGATCACA GAGCCTCCAT TTCTACAAGT 
CATGAGGCTG GAAACCAAAC AGCAGTTCCT AAGGACTCAA GTCTGGTCTC TGCCAGTGAC 
GGCCCAGTGG GGGCTTCCTC TCCACCATTG ACAGGATCGC TCTCCTGTCA GCCCGAAGTC 
ACCCAGCTGC CAGAGTGTAA GAGCACAGGC CAGGAAACTC CGCCAAAACA AAGCCTTGTG 
GCTGATCAGA AACTACCTGT TCTTCCAGGA CGTGCTACCC GCTCAAGCCA GTCTCCAAAA 
AAGCCGTTCA ACAGTATTAT TGAGCACCTG TCAGTGGTAT TCCCATGTTA CAACAGCACT 
GAGCTTGCTG GTTTTATTAA AAAAGTGCGA AACAAGAACA AAAACTCGCT CTCAGGATTG 
AGTATTGATG AAATTGTCCA AAGAGTGACA GAACACATTC TAGATGAACA GAAAAAGAAA 
AAGCCAAATT CAAATTCAGC AAAGGACAAG AGGTTGTCTG AGCCCAGCTC CACTGCTTCT 
GTGAACAGGG CCTCCCAGGG TCCACCCTCC GTGACTGCTG GACCATCGCC CAAAACCAAG 
GGACAAAAGA CAGAAGATGC CCCTGCATCG GGTGCAGCTT CCTGTGAACT GTGCCATGAG 
ATATTCAAAT CCAAAAACGT GCGTGTACTA AAATGTGGGC ACAAGTTTCA CAAAGGGTGC 
TTTAAGCAGT GGCTGAAGGG GCAGAGCACT TGCCCGGCCT GCCTCGATCA GGATCTCCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965204.1
CDS	1936..7938
Translation	MDDFALGDFTIADDALSEDCPYVDGCVFAPEFMSNDYVRVTQLYCDGVGMQYKDFTQSERNLEFDICNMWCSKPVSVLQDYCDAIKIYIFWPLLFQRQHSSIISRLHPCVEATNSHASEISLKKLQHLELMEDIVDLAKKVVNDSFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILPKLGAVDNCWPMLSIFFTEYKYHITKVVTENCNLLEEFKTQNCAECIEQGELMKMKGNEEFSKERFDIAIIYYTRAIEYRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKSNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPTRNPIKAFYESRAYIPSLSAPAFSTSLNFVETEKDSRKTNREMANGGNQNLKVVDEALKVDDCDCHPEFLPPSGQTPKYKGKQKSRNNELEKFSSSSQGSLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFIEECRFPPVPDAICCYQKCRGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKIQKKEAKKLAQERMEEDLRESNPPKTEEQKETVDSVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLSTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRIFLHVLSELKEVEPKLAAWIQKLNSFGLDATGPFFSRYGASLKELDFSIMTFLWNEKYGHKLASIEGKQLDYFCEPTSVKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSAKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETITSGEEHNRRNSNSAGPFVVPDHLRQDVEEFEAIYEQHSSEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKSFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPMSDLSSATASENVKPKPVAANSPKSTCEVMKPRPVSDNSSRSVSEDEKPKEVSSDSAQPVSEDASHKRVSSNSPKPASEDVKPAYWTQPHLVTGYCTYLPFRGFDITQTPPTYINVLPTLPQYTNIYTPLPNVSSEYQLQRSVPVVPSFIPSERLDESATVYFEGHHLNAENAPGNQIVPKTQILQDSMEVSVKTECSTDGADTALSESNRYDGHCGNSANKWEVNPEKNNEVTNTPHTQMVAIQVSWNITHQEVNTEPYDPFETQQGDISQIEKEFQVLQEQLNEACENYEQRKLKGSEETRDLEEKLKRNLEENKISKTELDWFLEDLEKEIKKWQQEKKEIQESLKALTKKMRKVLNANEIYAQKNDGKEKEHELLLDQSLEISNTFTNEKMKVEECIKKGKENYEESLQRAVAAEVSVLENWKDIEVYKLQIMESQAEGYLKNLRLLSSDSATYPDKESDIHSWESFLSKVKKEIEKAKSQYEEQIQAIKNGSRLSELSKVQVPELSFPVCSTILPTSLPESSGHEDHRASISTSHEAGNQTAVPKDSSLVSASDGPVGASSPPLTGSLSCQPEVTQLPECKSTGQETPPKQSLVADQKLPVLPGRATRSSQSPKKPFNSIIEHLSVVFPCYNSTELAGFIKKVRNKNKNSLSGLSIDEIVQRVTEHILDEQKKKKPNSNSAKDKRLSEPSSTASVNRASQGPPSVTAGPSPKTKGQKTEDAPASGAASCELCHEIFKSKNVRVLKCGHKFHKGCFKQWLKGQSTCPACLDQDLL

Target ORF information:

RefSeq Version	XM_014109729.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109729.1

ORF Insert Sequence:

ATGGATGATT TTGCCCTGGG AGATTTCACT ATAGCAGATG ATGCTTTGTC AGAAGATTGC 
CCTTATGTGG ACGGTTGTGT CTTTGCTCCT GAATTTATGT CCAATGATTA TGTTCGTGTG 
ACTCAACTTT ACTGCGATGG GGTGGGTATG CAGTATAAAG ATTTTACCCA AAGTGAGAGA 
AACTTAGAAT TTGACATCTG TAATATGTGG TGTAGTAAAC CAGTTTCTGT CCTGCAAGAT 
TATTGTGATG CCATTAAAAT ATACATCTTC TGGCCACTTC TGTTTCAACG TCAGCATAGT 
TCTATAATAT CACGGTTACA CCCCTGTGTG GAGGCCACCA ACTCTCATGC TTCTGAGATA 
AGTTTGAAGA AATTGCAACA TCTAGAATTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTCGTGAATG ATTCATTCTT TATTGGGGGC TTATTGAGGA TTGGTTATAA AATAGAAAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT ATACAGGCGA TGTAACAATT 
CTACCTAAAT TAGGAGCAGT TGACAATTGT TGGCCTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AGTTGTAACA GAAAACTGCA ATTTGCTTGA AGAATTTAAA 
ACTCAAAATT GTGCGGAATG TATAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCAA AAGAAAGATT TGATATAGCT ATTATCTATT ACACCAGAGC CATTGAATAT 
AGACCTGAAA ATCACCTTCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG CACTGGACAG 
TTTAGAAATG CACTTGGTGA TGGAAAGAGA GCCACTATCC TGAAGAGCAA TTGGACGAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCGAAC ACCAACTAGA 
AATCCAATTA AAGCCTTTTA TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAGATT CCAGAAAAAC TAACCGCGAA 
ATGGCAAACG GTGGTAATCA GAATCTAAAG GTGGTAGATG AAGCTTTGAA GGTAGATGAT 
TGTGACTGTC ATCCTGAATT TCTACCACCA TCAGGTCAGA CTCCAAAATA CAAAGGAAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGTTCTA GTTCACAGGG GAGTTTACCA 
GTAGATTTGA AGAACATTTT GGAAAAACAA TTCTCTAAAT CTTCCAGAGC TGCACATCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGTTA CACGGCTTTG 
TTGGAGCAGC GTTGCCGCAG TGCTGCACAG GCCTTCACAG AATTGCTCAA TGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAACCTGGCC ATGATTAACT ATGTCTTAGT AGTCTACGGA 
CTTGCTATTT CTCTCCTTGG AATAGGACAG CCTGAGGAAT TATCTGAAGC TGAAAACCAG 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGTTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAGAACAGA TTTCTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATTTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGATT 
ATTGAAGAGA CTCAGCCGGG GAAAATAAAG ATGCTGTTAG AGAAGTTTAT TGAAGAATGC 
AGGTTCCCTC CAGTGCCAGA TGCCATTTGT TGCTATCAGA AGTGCCGTGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGAATCAG CTGCTGCCAG 
TACTGTAAAA TAGAATTTCA CATGAACTGT TGGAAAAAGT TGAAAACAAC AACTTTTAAT 
GATAAAATTG ACAAGGATTT TCTACAAGGA ATTTGTCTTA CCCCTGACTG TGAAGGAATC 
ATTTCTAAGA TTATCATTTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATAAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGCTAAGAC TGAAAGAAGA CAAAAAATTG AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA 
AAATTAGCAC AAGAAAGAAT GGAAGAGGAC CTAAGGGAAA GTAATCCACC CAAAACTGAA 
GAGCAGAAAG AAACTGTAGA CAGTGTTCAG AGTTGCCAGT TCCTTGACGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATA AAATCTGGTA TTCTGAACAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA AGCACAGAAG ACTACACAAC CTGTTTTTCT 
AGCAGAAATT TTCTAAATGA AGCGGTGGAC TACGTCATTC GTCACTTGAT TCAAGAAAAG 
AATAGAGTAA AGACTAGGAT ATTTCTGCAT GTTTTGAGCG AACTTAAAGA AGTGGAGCCA 
AAATTAGCCG CTTGGATCCA GAAACTTAAC AGCTTTGGTT TAGATGCCAC AGGACCTTTT 
TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA ACTAGCCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTGT 
GAGCCAACAT CAGTGAAGGA AGCACGGTGT TTAATATGGC TGCTAGAAGA GCATAGAGAC 
AAGTTCCCAG CGCTACACAA TGCTCTAGAT GAGTTCTTTG ACATAATGGA TAGCCGCTGT 
ACTGTATTAA GGAAACAAGA CAGTGGCGAT GTGCCGTTTA ATTCCGCCAA GATAAAAAAT 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TGGTGGGGTC TGGGACACCT 
TCTGTAACAC CAAATAATGA GACTATCACT TCGGGTGAAG AACATAACAG ACGCAATTCA 
AATTCTGCAG GGCCATTTGT GGTACCTGAC CACCTTCGGC AAGATGTAGA AGAATTTGAA 
GCTATCTATG AGCAGCACAG TAGTGAATAT GTTGTCCGTA ATAAAAAGCT GTGGGACATT 
AACCCAAAAC AAAAATGTTC AACTCTGTAT GATTATTTCT CTCAGTTGTT GGAGGAGCAT 
GGTCCCTTGG ACATGAGTAA TAAAATGTTC TCTGAAGAAT ATGAATTCTT CCCAGAAGAA 
ACTAGACAGA TACTAGAAAA AGCTGGAGGT TTGAAATCTT TCCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCTTTG AAGAAAGTTG CTTTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTG GAAGAAATTT CAAAAACAGG GGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAATGTCTG ATTTATCTTC AGCAACAGCT TCTGAGAATG TGAAGCCCAA ACCTGTAGCT 
GCTAATTCTC CCAAATCAAC CTGTGAAGTT ATGAAGCCAA GACCAGTGTC TGATAATTCT 
TCTAGATCAG TTTCTGAAGA TGAGAAGCCC AAAGAAGTCT CTTCTGATTC TGCCCAACCT 
GTATCTGAGG ATGCAAGTCA CAAGCGAGTA TCCTCTAATT CTCCTAAACC AGCTTCTGAG 
GATGTGAAAC CAGCCTACTG GACTCAGCCC CATTTGGTCA CAGGATACTG TACATACCTC 
CCCTTTCGGG GATTTGACAT TACCCAGACA CCACCAACAT ACATAAATGT GTTACCAACT 
TTGCCCCAGT ACACTAACAT TTACACACCT CTGCCCAATG TTTCTTCTGA ATATCAGCTG 
CAAAGATCAG TGCCAGTGGT GCCGTCTTTT ATACCCAGTG AAAGACTAGA TGAAAGTGCT 
ACTGTGTACT TTGAGGGTCA TCATTTGAAT GCTGAGAATG CCCCTGGGAA CCAGATTGTC 
CCTAAAACAC AGATCCTTCA GGACTCTATG GAAGTGTCAG TAAAGACAGA GTGCAGCACA 
GATGGTGCTG ATACAGCCCT TAGTGAGTCT AACAGATATG ATGGTCACTG TGGAAATTCT 
GCCAACAAGT GGGAAGTAAA TCCAGAAAAA AACAATGAAG TAACAAATAC TCCACATACA 
CAAATGGTTG CCATACAGGT GTCTTGGAAT ATAACACATC AAGAAGTCAA TACTGAGCCA 
TATGATCCTT TTGAGACACA ACAGGGAGAT ATTTCACAGA TTGAGAAGGA GTTCCAGGTT 
TTACAAGAGC AGCTTAACGA GGCATGTGAA AATTATGAGC AGAGAAAACT CAAGGGCTCA 
GAAGAGACCA GGGATCTGGA AGAAAAATTG AAAAGGAACT TAGAAGAAAA CAAGATCTCA 
AAGACAGAAT TAGATTGGTT CCTTGAAGAC TTGGAAAAGG AAATCAAGAA ATGGCAACAG 
GAGAAAAAAG AAATCCAAGA AAGTCTGAAA GCGCTAACAA AGAAAATGAG AAAGGTTTTA 
AATGCCAACG AGATATATGC CCAGAAAAAT GATGGAAAGG AAAAGGAACA TGAATTACTT 
CTGGATCAGT CCCTTGAAAT CAGTAATACG TTTACAAATG AGAAAATGAA AGTAGAAGAA 
TGTATAAAGA AAGGGAAGGA GAATTATGAA GAGAGTCTTC AAAGAGCTGT GGCTGCAGAG 
GTATCTGTGC TTGAAAACTG GAAGGACATT GAAGTGTACA AATTACAGAT CATGGAATCA 
CAAGCAGAAG GGTATCTTAA GAACCTGAGG CTACTGAGCA GTGATTCTGC AACTTACCCT 
GATAAGGAAT CTGATATACA TTCATGGGAA TCATTTCTTT CTAAGGTTAA AAAAGAAATT 
GAAAAAGCAA AGTCTCAGTA TGAAGAACAA ATTCAAGCAA TTAAAAATGG CTCTCGACTC 
AGTGAACTTT CTAAAGTGCA AGTTCCTGAG CTTTCATTTC CTGTCTGTAG CACAATTCTT 
CCTACATCAC TTCCTGAGTC TTCAGGCCAT GAAGATCACA GAGCCTCCAT TTCTACAAGT 
CATGAGGCTG GAAACCAAAC AGCAGTTCCT AAGGACTCAA GTCTGGTCTC TGCCAGTGAC 
GGCCCAGTGG GGGCTTCCTC TCCACCATTG ACAGGATCGC TCTCCTGTCA GCCCGAAGTC 
ACCCAGCTGC CAGAGTGTAA GAGCACAGGC CAGGAAACTC CGCCAAAACA AAGCCTTGTG 
GCTGATCAGA AACTACCTGT TCTTCCAGGA CGTGCTACCC GCTCAAGCCA GTCTCCAAAA 
AAGCCGTTCA ACAGTATTAT TGAGCACCTG TCAGTGGTAT TCCCATGTTA CAACAGCACT 
GAGCTTGCTG GTTTTATTAA AAAAGTGCGA AACAAGAACA AAAACTCGCT CTCAGGATTG 
AGTATTGATG AAATTGTCCA AAGAGTGACA GAACACATTC TAGATGAACA GAAAAAGAAA 
AAGCCAAATT CAAATTCAGC AAAGGACAAG AGGTTGTCTG AGCCCAGCTC CACTGCTTCT 
GTGAACAGGG CCTCCCAGGG TCCACCCTCC GTGACTGCTG GACCATCGCC CAAAACCAAG 
GGACAAAAGA CAGAAGATGC CCCTGCATCG GGTGCAGCTT CCTGTGAACT GTGCCATGAG 
ATATTCAAAT CCAAAAACGT GCGTGTACTA AAATGTGGGC ACAAGTTTCA CAAAGGGTGC 
TTTAAGCAGT GGCTGAAGGG GCAGAGCACT TGCCCGGCCT GCCTCGATCA GGATCTCCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_005638905.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638962.1 Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

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CloneID	OCe29424
Clone ID Related Accession (Same CDS sequence)	XM_005638905.2 , XM_014109729.1 , XM_005638905.3
Accession Version	XM_005638905.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATT TTGCCCTGGG AGATTTCACT ATAGCAGATG ATGCTTTGTC AGAAGATTGC 
CCTTATGTGG ACGGTTGTGT CTTTGCTCCT GAATTTATGT CCAATGATTA TGTTCGTGTG 
ACTCAACTTT ACTGCGATGG GGTGGGTATG CAGTATAAAG ATTTTACCCA AAGTGAGAGA 
AACTTAGAAT TTGACATCTG TAATATGTGG TGTAGTAAAC CAGTTTCTGT CCTGCAAGAT 
TATTGTGATG CCATTAAAAT ATACATCTTC TGGCCACTTC TGTTTCAACG TCAGCATAGT 
TCTATAATAT CACGGTTACA CCCCTGTGTG GAGGCCACCA ACTCTCATGC TTCTGAGATA 
AGTTTGAAGA AATTGCAACA TCTAGAATTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTCGTGAATG ATTCATTCTT TATTGGGGGC TTATTGAGGA TTGGTTATAA AATAGAAAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT ATACAGGCGA TGTAACAATT 
CTACCTAAAT TAGGAGCAGT TGACAATTGT TGGCCTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AGTTGTAACA GAAAACTGCA ATTTGCTTGA AGAATTTAAA 
ACTCAAAATT GTGCGGAATG TATAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCAA AAGAAAGATT TGATATAGCT ATTATCTATT ACACCAGAGC CATTGAATAT 
AGACCTGAAA ATCACCTTCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG CACTGGACAG 
TTTAGAAATG CACTTGGTGA TGGAAAGAGA GCCACTATCC TGAAGAGCAA TTGGACGAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCGAAC ACCAACTAGA 
AATCCAATTA AAGCCTTTTA TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAGATT CCAGAAAAAC TAACCGCGAA 
ATGGCAAACG GTGGTAATCA GAATCTAAAG GTGGTAGATG AAGCTTTGAA GGTAGATGAT 
TGTGACTGTC ATCCTGAATT TCTACCACCA TCAGGTCAGA CTCCAAAATA CAAAGGAAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGTTCTA GTTCACAGGG GAGTTTACCA 
GTAGATTTGA AGAACATTTT GGAAAAACAA TTCTCTAAAT CTTCCAGAGC TGCACATCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGTTA CACGGCTTTG 
TTGGAGCAGC GTTGCCGCAG TGCTGCACAG GCCTTCACAG AATTGCTCAA TGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAACCTGGCC ATGATTAACT ATGTCTTAGT AGTCTACGGA 
CTTGCTATTT CTCTCCTTGG AATAGGACAG CCTGAGGAAT TATCTGAAGC TGAAAACCAG 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGTTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAGAACAGA TTTCTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATTTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGATT 
ATTGAAGAGA CTCAGCCGGG GAAAATAAAG ATGCTGTTAG AGAAGTTTAT TGAAGAATGC 
AGGTTCCCTC CAGTGCCAGA TGCCATTTGT TGCTATCAGA AGTGCCGTGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGAATCAG CTGCTGCCAG 
TACTGTAAAA TAGAATTTCA CATGAACTGT TGGAAAAAGT TGAAAACAAC AACTTTTAAT 
GATAAAATTG ACAAGGATTT TCTACAAGGA ATTTGTCTTA CCCCTGACTG TGAAGGAATC 
ATTTCTAAGA TTATCATTTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATAAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGCTAAGAC TGAAAGAAGA CAAAAAATTG AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA 
AAATTAGCAC AAGAAAGAAT GGAAGAGGAC CTAAGGGAAA GTAATCCACC CAAAACTGAA 
GAGCAGAAAG AAACTGTAGA CAGTGTTCAG AGTTGCCAGT TCCTTGACGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATA AAATCTGGTA TTCTGAACAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA AGCACAGAAG ACTACACAAC CTGTTTTTCT 
AGCAGAAATT TTCTAAATGA AGCGGTGGAC TACGTCATTC GTCACTTGAT TCAAGAAAAG 
AATAGAGTAA AGACTAGGAT ATTTCTGCAT GTTTTGAGCG AACTTAAAGA AGTGGAGCCA 
AAATTAGCCG CTTGGATCCA GAAACTTAAC AGCTTTGGTT TAGATGCCAC AGGACCTTTT 
TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA ACTAGCCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTGT 
GAGCCAACAT CAGTGAAGGA AGCACGGTGT TTAATATGGC TGCTAGAAGA GCATAGAGAC 
AAGTTCCCAG CGCTACACAA TGCTCTAGAT GAGTTCTTTG ACATAATGGA TAGCCGCTGT 
ACTGTATTAA GGAAACAAGA CAGTGGCGAT GTGCCGTTTA ATTCCGCCAA GATAAAAAAT 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TGGTGGGGTC TGGGACACCT 
TCTGTAACAC CAAATAATGA GACTATCACT TCGGGTGAAG AACATAACAG ACGCAATTCA 
AATTCTGCAG GGCCATTTGT GGTACCTGAC CACCTTCGGC AAGATGTAGA AGAATTTGAA 
GCTATCTATG AGCAGCACAG TAGTGAATAT GTTGTCCGTA ATAAAAAGCT GTGGGACATT 
AACCCAAAAC AAAAATGTTC AACTCTGTAT GATTATTTCT CTCAGTTGTT GGAGGAGCAT 
GGTCCCTTGG ACATGAGTAA TAAAATGTTC TCTGAAGAAT ATGAATTCTT CCCAGAAGAA 
ACTAGACAGA TACTAGAAAA AGCTGGAGGT TTGAAATCTT TCCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCTTTG AAGAAAGTTG CTTTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTG GAAGAAATTT CAAAAACAGG GGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAATGTCTG ATTTATCTTC AGCAACAGCT TCTGAGAATG TGAAGCCCAA ACCTGTAGCT 
GCTAATTCTC CCAAATCAAC CTGTGAAGTT ATGAAGCCAA GACCAGTGTC TGATAATTCT 
TCTAGATCAG TTTCTGAAGA TGAGAAGCCC AAAGAAGTCT CTTCTGATTC TGCCCAACCT 
GTATCTGAGG ATGCAAGTCA CAAGCGAGTA TCCTCTAATT CTCCTAAACC AGCTTCTGAG 
GATGTGAAAC CAGCCTACTG GACTCAGCCC CATTTGGTCA CAGGATACTG TACATACCTC 
CCCTTTCGGG GATTTGACAT TACCCAGACA CCACCAACAT ACATAAATGT GTTACCAACT 
TTGCCCCAGT ACACTAACAT TTACACACCT CTGCCCAATG TTTCTTCTGA ATATCAGCTG 
CAAAGATCAG TGCCAGTGGT GCCGTCTTTT ATACCCAGTG AAAGACTAGA TGAAAGTGCT 
ACTGTGTACT TTGAGGGTCA TCATTTGAAT GCTGAGAATG CCCCTGGGAA CCAGATTGTC 
CCTAAAACAC AGATCCTTCA GGACTCTATG GAAGTGTCAG TAAAGACAGA GTGCAGCACA 
GATGGTGCTG ATACAGCCCT TAGTGAGTCT AACAGATATG ATGGTCACTG TGGAAATTCT 
GCCAACAAGT GGGAAGTAAA TCCAGAAAAA AACAATGAAG TAACAAATAC TCCACATACA 
CAAATGGTTG CCATACAGGT GTCTTGGAAT ATAACACATC AAGAAGTCAA TACTGAGCCA 
TATGATCCTT TTGAGACACA ACAGGGAGAT ATTTCACAGA TTGAGAAGGA GTTCCAGGTT 
TTACAAGAGC AGCTTAACGA GGCATGTGAA AATTATGAGC AGAGAAAACT CAAGGGCTCA 
GAAGAGACCA GGGATCTGGA AGAAAAATTG AAAAGGAACT TAGAAGAAAA CAAGATCTCA 
AAGACAGAAT TAGATTGGTT CCTTGAAGAC TTGGAAAAGG AAATCAAGAA ATGGCAACAG 
GAGAAAAAAG AAATCCAAGA AAGTCTGAAA GCGCTAACAA AGAAAATGAG AAAGGTTTTA 
AATGCCAACG AGATATATGC CCAGAAAAAT GATGGAAAGG AAAAGGAACA TGAATTACTT 
CTGGATCAGT CCCTTGAAAT CAGTAATACG TTTACAAATG AGAAAATGAA AGTAGAAGAA 
TGTATAAAGA AAGGGAAGGA GAATTATGAA GAGAGTCTTC AAAGAGCTGT GGCTGCAGAG 
GTATCTGTGC TTGAAAACTG GAAGGACATT GAAGTGTACA AATTACAGAT CATGGAATCA 
CAAGCAGAAG GGTATCTTAA GAACCTGAGG CTACTGAGCA GTGATTCTGC AACTTACCCT 
GATAAGGAAT CTGATATACA TTCATGGGAA TCATTTCTTT CTAAGGTTAA AAAAGAAATT 
GAAAAAGCAA AGTCTCAGTA TGAAGAACAA ATTCAAGCAA TTAAAAATGG CTCTCGACTC 
AGTGAACTTT CTAAAGTGCA AGTTCCTGAG CTTTCATTTC CTGTCTGTAG CACAATTCTT 
CCTACATCAC TTCCTGAGTC TTCAGGCCAT GAAGATCACA GAGCCTCCAT TTCTACAAGT 
CATGAGGCTG GAAACCAAAC AGCAGTTCCT AAGGACTCAA GTCTGGTCTC TGCCAGTGAC 
GGCCCAGTGG GGGCTTCCTC TCCACCATTG ACAGGATCGC TCTCCTGTCA GCCCGAAGTC 
ACCCAGCTGC CAGAGTGTAA GAGCACAGGC CAGGAAACTC CGCCAAAACA AAGCCTTGTG 
GCTGATCAGA AACTACCTGT TCTTCCAGGA CGTGCTACCC GCTCAAGCCA GTCTCCAAAA 
AAGCCGTTCA ACAGTATTAT TGAGCACCTG TCAGTGGTAT TCCCATGTTA CAACAGCACT 
GAGCTTGCTG GTTTTATTAA AAAAGTGCGA AACAAGAACA AAAACTCGCT CTCAGGATTG 
AGTATTGATG AAATTGTCCA AAGAGTGACA GAACACATTC TAGATGAACA GAAAAAGAAA 
AAGCCAAATT CAAATTCAGC AAAGGACAAG AGGTTGTCTG AGCCCAGCTC CACTGCTTCT 
GTGAACAGGG CCTCCCAGGG TCCACCCTCC GTGACTGCTG GACCATCGCC CAAAACCAAG 
GGACAAAAGA CAGAAGATGC CCCTGCATCG GGTGCAGCTT CCTGTGAACT GTGCCATGAG 
ATATTCAAAT CCAAAAACGT GCGTGTACTA AAATGTGGGC ACAAGTTTCA CAAAGGGTGC 
TTTAAGCAGT GGCTGAAGGG GCAGAGCACT TGCCCGGCCT GCCTCGATCA GGATCTCCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638962.1
CDS	1606..7608
Translation	MDDFALGDFTIADDALSEDCPYVDGCVFAPEFMSNDYVRVTQLYCDGVGMQYKDFTQSERNLEFDICNMWCSKPVSVLQDYCDAIKIYIFWPLLFQRQHSSIISRLHPCVEATNSHASEISLKKLQHLELMEDIVDLAKKVVNDSFFIGGLLRIGYKIENKILAMEEALNWVKYTGDVTILPKLGAVDNCWPMLSIFFTEYKYHITKVVTENCNLLEEFKTQNCAECIEQGELMKMKGNEEFSKERFDIAIIYYTRAIEYRPENHLLYGNRALCFLRTGQFRNALGDGKRATILKSNWTKGHYRYCAALSMLGEYDWALQANIKAQKLCKNDPEGIKDLIQQHVKLQKQIEDLQGRTPTRNPIKAFYESRAYIPSLSAPAFSTSLNFVETEKDSRKTNREMANGGNQNLKVVDEALKVDDCDCHPEFLPPSGQTPKYKGKQKSRNNELEKFSSSSQGSLPVDLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYTALLEQRCRSAAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRMIEHYPNEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLICRLPGVLTWPTSNVIIEETQPGKIKMLLEKFIEECRFPPVPDAICCYQKCRGYSKIQIYITDPDFKGFIRISCCQYCKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGIISKIIIFSSGGQVKCEFEHKVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKIQKKEAKKLAQERMEEDLRESNPPKTEEQKETVDSVQSCQFLDDRILQCIKQYADKIKSGILNTSKLLKELLSWKVLSTEDYTTCFSSRNFLNEAVDYVIRHLIQEKNRVKTRIFLHVLSELKEVEPKLAAWIQKLNSFGLDATGPFFSRYGASLKELDFSIMTFLWNEKYGHKLASIEGKQLDYFCEPTSVKEARCLIWLLEEHRDKFPALHNALDEFFDIMDSRCTVLRKQDSGDVPFNSAKIKNKGKKKKPKDSKPMLVGSGTPSVTPNNETITSGEEHNRRNSNSAGPFVVPDHLRQDVEEFEAIYEQHSSEYVVRNKKLWDINPKQKCSTLYDYFSQLLEEHGPLDMSNKMFSEEYEFFPEETRQILEKAGGLKSFLLGCPRFVVIDNCIALKKVALRLKKKRKKKNIKTKVEEISKTGEYLRVKLPLNPTAREFKPDVKSKPMSDLSSATASENVKPKPVAANSPKSTCEVMKPRPVSDNSSRSVSEDEKPKEVSSDSAQPVSEDASHKRVSSNSPKPASEDVKPAYWTQPHLVTGYCTYLPFRGFDITQTPPTYINVLPTLPQYTNIYTPLPNVSSEYQLQRSVPVVPSFIPSERLDESATVYFEGHHLNAENAPGNQIVPKTQILQDSMEVSVKTECSTDGADTALSESNRYDGHCGNSANKWEVNPEKNNEVTNTPHTQMVAIQVSWNITHQEVNTEPYDPFETQQGDISQIEKEFQVLQEQLNEACENYEQRKLKGSEETRDLEEKLKRNLEENKISKTELDWFLEDLEKEIKKWQQEKKEIQESLKALTKKMRKVLNANEIYAQKNDGKEKEHELLLDQSLEISNTFTNEKMKVEECIKKGKENYEESLQRAVAAEVSVLENWKDIEVYKLQIMESQAEGYLKNLRLLSSDSATYPDKESDIHSWESFLSKVKKEIEKAKSQYEEQIQAIKNGSRLSELSKVQVPELSFPVCSTILPTSLPESSGHEDHRASISTSHEAGNQTAVPKDSSLVSASDGPVGASSPPLTGSLSCQPEVTQLPECKSTGQETPPKQSLVADQKLPVLPGRATRSSQSPKKPFNSIIEHLSVVFPCYNSTELAGFIKKVRNKNKNSLSGLSIDEIVQRVTEHILDEQKKKKPNSNSAKDKRLSEPSSTASVNRASQGPPSVTAGPSPKTKGQKTEDAPASGAASCELCHEIFKSKNVRVLKCGHKFHKGCFKQWLKGQSTCPACLDQDLL

Target ORF information:

RefSeq Version	XM_005638905.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638905.3

ORF Insert Sequence:

ATGGATGATT TTGCCCTGGG AGATTTCACT ATAGCAGATG ATGCTTTGTC AGAAGATTGC 
CCTTATGTGG ACGGTTGTGT CTTTGCTCCT GAATTTATGT CCAATGATTA TGTTCGTGTG 
ACTCAACTTT ACTGCGATGG GGTGGGTATG CAGTATAAAG ATTTTACCCA AAGTGAGAGA 
AACTTAGAAT TTGACATCTG TAATATGTGG TGTAGTAAAC CAGTTTCTGT CCTGCAAGAT 
TATTGTGATG CCATTAAAAT ATACATCTTC TGGCCACTTC TGTTTCAACG TCAGCATAGT 
TCTATAATAT CACGGTTACA CCCCTGTGTG GAGGCCACCA ACTCTCATGC TTCTGAGATA 
AGTTTGAAGA AATTGCAACA TCTAGAATTG ATGGAAGATA TTGTGGATTT GGCAAAGAAA 
GTCGTGAATG ATTCATTCTT TATTGGGGGC TTATTGAGGA TTGGTTATAA AATAGAAAAT 
AAAATCTTGG CAATGGAAGA AGCTTTGAAT TGGGTGAAAT ATACAGGCGA TGTAACAATT 
CTACCTAAAT TAGGAGCAGT TGACAATTGT TGGCCTATGT TAAGTATTTT CTTTACTGAA 
TACAAGTATC ACATAACTAA AGTTGTAACA GAAAACTGCA ATTTGCTTGA AGAATTTAAA 
ACTCAAAATT GTGCGGAATG TATAGAGCAA GGAGAACTAA TGAAAATGAA AGGAAATGAA 
GAGTTTTCAA AAGAAAGATT TGATATAGCT ATTATCTATT ACACCAGAGC CATTGAATAT 
AGACCTGAAA ATCACCTTCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG CACTGGACAG 
TTTAGAAATG CACTTGGTGA TGGAAAGAGA GCCACTATCC TGAAGAGCAA TTGGACGAAG 
GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA 
GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT 
CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCGAAC ACCAACTAGA 
AATCCAATTA AAGCCTTTTA TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA 
TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAGATT CCAGAAAAAC TAACCGCGAA 
ATGGCAAACG GTGGTAATCA GAATCTAAAG GTGGTAGATG AAGCTTTGAA GGTAGATGAT 
TGTGACTGTC ATCCTGAATT TCTACCACCA TCAGGTCAGA CTCCAAAATA CAAAGGAAAA 
CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGTTCTA GTTCACAGGG GAGTTTACCA 
GTAGATTTGA AGAACATTTT GGAAAAACAA TTCTCTAAAT CTTCCAGAGC TGCACATCAG 
GATTTTGCTA ATATAATGAA AATGCTGAGA AGCTTAATCC AGGATGGTTA CACGGCTTTG 
TTGGAGCAGC GTTGCCGCAG TGCTGCACAG GCCTTCACAG AATTGCTCAA TGGTTTAGAT 
CCTCAAAAAA TAAAGCAATT GAACCTGGCC ATGATTAACT ATGTCTTAGT AGTCTACGGA 
CTTGCTATTT CTCTCCTTGG AATAGGACAG CCTGAGGAAT TATCTGAAGC TGAAAACCAG 
TTTAAGAGGA TGATTGAACA CTACCCCAAT GAGGGACTTG ATTGTTTGGC CTACTGTGGA 
ATTGGAAAAG TATATTTGAA AAAGAACAGA TTTCTAGAAG CTCTTAATCA TTTTGAGAAA 
GCAAGAACCT TGATTTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGATT 
ATTGAAGAGA CTCAGCCGGG GAAAATAAAG ATGCTGTTAG AGAAGTTTAT TGAAGAATGC 
AGGTTCCCTC CAGTGCCAGA TGCCATTTGT TGCTATCAGA AGTGCCGTGG ATATTCCAAA 
ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGAATCAG CTGCTGCCAG 
TACTGTAAAA TAGAATTTCA CATGAACTGT TGGAAAAAGT TGAAAACAAC AACTTTTAAT 
GATAAAATTG ACAAGGATTT TCTACAAGGA ATTTGTCTTA CCCCTGACTG TGAAGGAATC 
ATTTCTAAGA TTATCATTTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG 
GTCATAAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG 
AAGCTAAGAC TGAAAGAAGA CAAAAAATTG AAGAGAAAGA TCCAAAAAAA AGAAGCAAAA 
AAATTAGCAC AAGAAAGAAT GGAAGAGGAC CTAAGGGAAA GTAATCCACC CAAAACTGAA 
GAGCAGAAAG AAACTGTAGA CAGTGTTCAG AGTTGCCAGT TCCTTGACGA CAGAATTCTG 
CAGTGCATAA AGCAGTACGC TGACAAGATA AAATCTGGTA TTCTGAACAC TTCCAAGCTT 
CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA AGCACAGAAG ACTACACAAC CTGTTTTTCT 
AGCAGAAATT TTCTAAATGA AGCGGTGGAC TACGTCATTC GTCACTTGAT TCAAGAAAAG 
AATAGAGTAA AGACTAGGAT ATTTCTGCAT GTTTTGAGCG AACTTAAAGA AGTGGAGCCA 
AAATTAGCCG CTTGGATCCA GAAACTTAAC AGCTTTGGTT TAGATGCCAC AGGACCTTTT 
TTTTCTCGTT ATGGAGCCTC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG 
AATGAGAAAT ATGGTCATAA ACTAGCCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTGT 
GAGCCAACAT CAGTGAAGGA AGCACGGTGT TTAATATGGC TGCTAGAAGA GCATAGAGAC 
AAGTTCCCAG CGCTACACAA TGCTCTAGAT GAGTTCTTTG ACATAATGGA TAGCCGCTGT 
ACTGTATTAA GGAAACAAGA CAGTGGCGAT GTGCCGTTTA ATTCCGCCAA GATAAAAAAT 
AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TGGTGGGGTC TGGGACACCT 
TCTGTAACAC CAAATAATGA GACTATCACT TCGGGTGAAG AACATAACAG ACGCAATTCA 
AATTCTGCAG GGCCATTTGT GGTACCTGAC CACCTTCGGC AAGATGTAGA AGAATTTGAA 
GCTATCTATG AGCAGCACAG TAGTGAATAT GTTGTCCGTA ATAAAAAGCT GTGGGACATT 
AACCCAAAAC AAAAATGTTC AACTCTGTAT GATTATTTCT CTCAGTTGTT GGAGGAGCAT 
GGTCCCTTGG ACATGAGTAA TAAAATGTTC TCTGAAGAAT ATGAATTCTT CCCAGAAGAA 
ACTAGACAGA TACTAGAAAA AGCTGGAGGT TTGAAATCTT TCCTCCTGGG ATGTCCTCGT 
TTTGTTGTGA TTGACAACTG TATTGCTTTG AAGAAAGTTG CTTTACGGCT CAAGAAAAAG 
AGAAAGAAGA AAAACATTAA GACAAAAGTG GAAGAAATTT CAAAAACAGG GGAGTATTTA 
CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA 
CCAATGTCTG ATTTATCTTC AGCAACAGCT TCTGAGAATG TGAAGCCCAA ACCTGTAGCT 
GCTAATTCTC CCAAATCAAC CTGTGAAGTT ATGAAGCCAA GACCAGTGTC TGATAATTCT 
TCTAGATCAG TTTCTGAAGA TGAGAAGCCC AAAGAAGTCT CTTCTGATTC TGCCCAACCT 
GTATCTGAGG ATGCAAGTCA CAAGCGAGTA TCCTCTAATT CTCCTAAACC AGCTTCTGAG 
GATGTGAAAC CAGCCTACTG GACTCAGCCC CATTTGGTCA CAGGATACTG TACATACCTC 
CCCTTTCGGG GATTTGACAT TACCCAGACA CCACCAACAT ACATAAATGT GTTACCAACT 
TTGCCCCAGT ACACTAACAT TTACACACCT CTGCCCAATG TTTCTTCTGA ATATCAGCTG 
CAAAGATCAG TGCCAGTGGT GCCGTCTTTT ATACCCAGTG AAAGACTAGA TGAAAGTGCT 
ACTGTGTACT TTGAGGGTCA TCATTTGAAT GCTGAGAATG CCCCTGGGAA CCAGATTGTC 
CCTAAAACAC AGATCCTTCA GGACTCTATG GAAGTGTCAG TAAAGACAGA GTGCAGCACA 
GATGGTGCTG ATACAGCCCT TAGTGAGTCT AACAGATATG ATGGTCACTG TGGAAATTCT 
GCCAACAAGT GGGAAGTAAA TCCAGAAAAA AACAATGAAG TAACAAATAC TCCACATACA 
CAAATGGTTG CCATACAGGT GTCTTGGAAT ATAACACATC AAGAAGTCAA TACTGAGCCA 
TATGATCCTT TTGAGACACA ACAGGGAGAT ATTTCACAGA TTGAGAAGGA GTTCCAGGTT 
TTACAAGAGC AGCTTAACGA GGCATGTGAA AATTATGAGC AGAGAAAACT CAAGGGCTCA 
GAAGAGACCA GGGATCTGGA AGAAAAATTG AAAAGGAACT TAGAAGAAAA CAAGATCTCA 
AAGACAGAAT TAGATTGGTT CCTTGAAGAC TTGGAAAAGG AAATCAAGAA ATGGCAACAG 
GAGAAAAAAG AAATCCAAGA AAGTCTGAAA GCGCTAACAA AGAAAATGAG AAAGGTTTTA 
AATGCCAACG AGATATATGC CCAGAAAAAT GATGGAAAGG AAAAGGAACA TGAATTACTT 
CTGGATCAGT CCCTTGAAAT CAGTAATACG TTTACAAATG AGAAAATGAA AGTAGAAGAA 
TGTATAAAGA AAGGGAAGGA GAATTATGAA GAGAGTCTTC AAAGAGCTGT GGCTGCAGAG 
GTATCTGTGC TTGAAAACTG GAAGGACATT GAAGTGTACA AATTACAGAT CATGGAATCA 
CAAGCAGAAG GGTATCTTAA GAACCTGAGG CTACTGAGCA GTGATTCTGC AACTTACCCT 
GATAAGGAAT CTGATATACA TTCATGGGAA TCATTTCTTT CTAAGGTTAA AAAAGAAATT 
GAAAAAGCAA AGTCTCAGTA TGAAGAACAA ATTCAAGCAA TTAAAAATGG CTCTCGACTC 
AGTGAACTTT CTAAAGTGCA AGTTCCTGAG CTTTCATTTC CTGTCTGTAG CACAATTCTT 
CCTACATCAC TTCCTGAGTC TTCAGGCCAT GAAGATCACA GAGCCTCCAT TTCTACAAGT 
CATGAGGCTG GAAACCAAAC AGCAGTTCCT AAGGACTCAA GTCTGGTCTC TGCCAGTGAC 
GGCCCAGTGG GGGCTTCCTC TCCACCATTG ACAGGATCGC TCTCCTGTCA GCCCGAAGTC 
ACCCAGCTGC CAGAGTGTAA GAGCACAGGC CAGGAAACTC CGCCAAAACA AAGCCTTGTG 
GCTGATCAGA AACTACCTGT TCTTCCAGGA CGTGCTACCC GCTCAAGCCA GTCTCCAAAA 
AAGCCGTTCA ACAGTATTAT TGAGCACCTG TCAGTGGTAT TCCCATGTTA CAACAGCACT 
GAGCTTGCTG GTTTTATTAA AAAAGTGCGA AACAAGAACA AAAACTCGCT CTCAGGATTG 
AGTATTGATG AAATTGTCCA AAGAGTGACA GAACACATTC TAGATGAACA GAAAAAGAAA 
AAGCCAAATT CAAATTCAGC AAAGGACAAG AGGTTGTCTG AGCCCAGCTC CACTGCTTCT 
GTGAACAGGG CCTCCCAGGG TCCACCCTCC GTGACTGCTG GACCATCGCC CAAAACCAAG 
GGACAAAAGA CAGAAGATGC CCCTGCATCG GGTGCAGCTT CCTGTGAACT GTGCCATGAG 
ATATTCAAAT CCAAAAACGT GCGTGTACTA AAATGTGGGC ACAAGTTTCA CAAAGGGTGC 
TTTAAGCAGT GGCTGAAGGG GCAGAGCACT TGCCCGGCCT GCCTCGATCA GGATCTCCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TTC3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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