COL20A1 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_009437613.2	XP_009435888.2	collagen alpha-1(XX) chain isoform X1
XM_009437616.2	XP_009435891.2	collagen alpha-1(XX) chain isoform X4
XM_016938329.1	XP_016793818.1	collagen alpha-1(XX) chain isoform X9
XM_009437623.2	XP_009435898.1	collagen alpha-1(XX) chain isoform X10
XM_009437625.2	XP_009435900.2	collagen alpha-1(XX) chain isoform X13
XM_009437613.3	XP_009435888.2	collagen alpha-1(XX) chain isoform X1
XM_009437621.3	XP_009435896.2	collagen alpha-1(XX) chain isoform X6
XM_009437622.3	XP_009435897.2	collagen alpha-1(XX) chain isoform X7
XM_016938328.2	XP_016793817.1	collagen alpha-1(XX) chain isoform X8
XM_009437623.3	XP_009435898.1	collagen alpha-1(XX) chain isoform X10
XM_009437625.3	XP_009435900.2	collagen alpha-1(XX) chain isoform X13
XM_009437615.2	XP_009435890.2	collagen alpha-1(XX) chain isoform X3
XM_009437624.3	XP_009435899.2	collagen alpha-1(XX) chain isoform X12
XM_009437626.3	XP_009435901.2	collagen alpha-1(XX) chain isoform X14
XM_009437620.2	XP_009435895.2	collagen alpha-1(XX) chain isoform X5
XM_009437621.2	XP_009435896.2	collagen alpha-1(XX) chain isoform X6
XM_009437624.2	XP_009435899.2	collagen alpha-1(XX) chain isoform X12
XM_009437615.3	XP_009435890.2	collagen alpha-1(XX) chain isoform X3
XM_009437616.3	XP_009435891.2	collagen alpha-1(XX) chain isoform X4
XM_009437620.3	XP_009435895.2	collagen alpha-1(XX) chain isoform X5
XM_016938330.2	XP_016793819.1	collagen alpha-1(XX) chain isoform X11
XM_009437614.2	XP_009435889.2	collagen alpha-1(XX) chain isoform X2
XM_009437622.2	XP_009435897.2	collagen alpha-1(XX) chain isoform X7
XM_016938328.1	XP_016793817.1	collagen alpha-1(XX) chain isoform X8
XM_009437626.2	XP_009435901.2	collagen alpha-1(XX) chain isoform X14
XM_016938329.2	XP_016793818.1	collagen alpha-1(XX) chain isoform X9
XM_016938330.1	XP_016793819.1	collagen alpha-1(XX) chain isoform X11
XM_009437614.3	XP_009435889.2	collagen alpha-1(XX) chain isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc102059	XM_009437613.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102060	XM_009437614.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102061	XM_009437615.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102062	XM_009437616.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102063	XM_009437620.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102064	XM_009437621.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102065	XM_009437622.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102066	XM_016938328.2 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102067	XM_016938329.2 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102068	XM_009437623.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102069	XM_016938330.2 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102070	XM_009437624.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc102071	XM_009437625.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc102072	XM_009437626.3 Latest version!	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc77414	XM_009437613.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77415	XM_009437614.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77416	XM_009437615.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77417	XM_009437616.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77418	XM_009437620.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77419	XM_009437621.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77420	XM_009437622.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77421	XM_016938328.1 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77422	XM_016938329.1 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77423	XM_016938330.1 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77424	XM_009437624.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc77425	XM_009437625.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPc77426	XM_009437626.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPc47818	XM_009437623.2 Latest version!	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

COL20A1 ( XM_009437613.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435888.2 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc102059
Clone ID Related Accession (Same CDS sequence)	XM_009437613.3
Accession Version	XM_009437613.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437613.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTC ACGTGTCAAA GTTCGACTCC TTCCACAAGA 
ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT GAGCCCCTGG 
GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT GGTGGCCGCC 
ACCTTGAGGG CAGAGGGGAG CCTGGAGCTG TTGGTCAGAC GGGCAGCCCT GGGCAGCAGG 
GGGCTAGCAC CCAGGGCCTC TGGGAGTGAC AGGACATTTT CTGCACTGCC CCGAGGAACG 
CTGAGCCTTC CTCCCTGGGT TTCTCTGGAC ACCGAGAGCG ACCACATCCT GGAGAAGCCA 
GGAGAAAAGC TCAGGAAGAG CCTGCAGGTG GAAGGAGAGA GAAGCAGCGG CCTCGGCCAA 
GGCCCACCCC ATGCTCTTGG CTCTGTAGCA TTTCCAAGTT CAGATAAACC CCTGAGTGCT 
CACCCAGCCA AGCGAGGCGG GAGTCATTTC CTGGGAGAGT GTCCCCCACC CTGGCTCCGT 
ACACCCCACC TTGAGGCCCT GCAGCTTCAG CCGCAGTGGG GAAGGGGAGG GGCTGGGTTG 
ATGGCGAATT GGGTTCAGGA GGGCAGCCGT GCCCTGTGGC CATCTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435888.2
CDS	145..4455
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAEGSLELLVRRAALGSRGLAPRASGSDRTFSALPRGTLSLPPWVSLDTESDHILEKPGEKLRKSLQVEGERSSGLGQGPPHALGSVAFPSSDKPLSAHPAKRGGSHFLGECPPPWLRTPHLEALQLQPQWGRGGAGLMANWVQEGSRALWPSQ

Target ORF information:

RefSeq Version	XM_009437613.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437613.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTC ACGTGTCAAA GTTCGACTCC TTCCACAAGA 
ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT GAGCCCCTGG 
GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT GGTGGCCGCC 
ACCTTGAGGG CAGAGGGGAG CCTGGAGCTG TTGGTCAGAC GGGCAGCCCT GGGCAGCAGG 
GGGCTAGCAC CCAGGGCCTC TGGGAGTGAC AGGACATTTT CTGCACTGCC CCGAGGAACG 
CTGAGCCTTC CTCCCTGGGT TTCTCTGGAC ACCGAGAGCG ACCACATCCT GGAGAAGCCA 
GGAGAAAAGC TCAGGAAGAG CCTGCAGGTG GAAGGAGAGA GAAGCAGCGG CCTCGGCCAA 
GGCCCACCCC ATGCTCTTGG CTCTGTAGCA TTTCCAAGTT CAGATAAACC CCTGAGTGCT 
CACCCAGCCA AGCGAGGCGG GAGTCATTTC CTGGGAGAGT GTCCCCCACC CTGGCTCCGT 
ACACCCCACC TTGAGGCCCT GCAGCTTCAG CCGCAGTGGG GAAGGGGAGG GGCTGGGTTG 
ATGGCGAATT GGGTTCAGGA GGGCAGCCGT GCCCTGTGGC CATCTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437614.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435889.2 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA.

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CloneID	OPc102060
Clone ID Related Accession (Same CDS sequence)	XM_009437614.3
Accession Version	XM_009437614.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437614.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC GGCTCCAGCC 
CTGGACACCC TCCCTGCCCC CACCAGCCTG GTCCTGAGCC AGGTGACCTC CTCCAGCATC 
CGCCTGTCCT GGACTCCAGC CCCCCGGCAC CCCCTCAAGT ATCTGATCGT TTGGCGAGCC 
TCTAGAGGTG GCACCCCCAG GGAGGTGGTG GTGGAGGGGC CCGCCGCCTC CACGGAGCTG 
CACAACCTGG CCTCCCGCAC AGAGTACCTG GTCTCCGTGT TCCCCATCTA TGAGGGCGGG 
GTTGGCGAAG GCCTGCGGGG CCTGGTGACC ACAGCACCTC TGCCTCCGCC CCGGGCGCTG 
ACCCTGGCCG CAGTGACGCC CAGAACCATC CACCTCACCT GGCAGCCCTC GGCCGGGGCC 
ACCCACTACC TGGTGCGATG TTCTCCTGCT TCCCCCAAGG GTGAGGAGGA GGAGCGAGAG 
GTGCAGGTCG GGCGGCCCGA GGTGCTGCTG GATGGCCTGG AACCTGGCAG GGACTATGAG 
GTCTCGGTGC AGAGCCTGCG AGGCCCTGAG GGCAGCGAGG CCCGGGGCAT CCATGCCAGG 
ACCCCCGCCC TGGCCCCCCC GAGACACCTG GGCTTCTCAG ACGTGAGCCA CGACGCGGCA 
CGAGTGTTCT GGGAGGGTGC CCCAAGGCCT GTGCGCCTGG TCAGGGTCAC CTATGTCTCC 
AGCGAGGGTG GACACTCGGG GCAGACAGAG GCTCCTGGGA ACGCCACCTC GGCCACGCTG 
GGGCCTCTCT CTTCCTCCAC CACCTACACT GTCCGTGTCA CCTGCCTCTA CCCTGGGGGT 
GGCTCCTCTA CGCTGACTGG CCGGGTGACC ACCAAGAAAG CTCCCAGCCC AAGCCAGCTG 
TCCGTGACGG AGCTGCCAGG CGACGCAGTC CAGCTGGCGT GGGTGGCCGC AGCCCCGTCT 
GGCGTGCTTG TCTACCAGAT CACATGGACG CCCCTGGGAG AGGGGAAGGC TCACGAGATC 
TCTGTCCCAG GGAACCTCGG CACGGCCGTC CTGCCTGGCC TAGGGAGGCA CACAGAGTAC 
AACGTCACCA TCTTGGCCTA CTACAGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGC 
TATACCCCCT CCACGGTGAG CAGGAGCCCA CCCTCCAACC TGGCCCTGGC CTCGGAGACC 
CCCGACAGCC TGCAGGTCAG CTGGACACCC CCGCTTGGCC ACGTGCTCCA TTACTGGCTC 
ACCTACGCCC CCGCCTCTGG CTTGGGACCT GAGAAATCCG TCTCTGTGCC AGCACCCAGG 
AGCCACGTGA CACTGCCCGA CCTGCAGGCA GCCACGAAGT ACAGGGTCCT GCTCTCAGCT 
ATCTATGCAG CAGGCAGGAG TGAGGCTGTG TCTGCCATGG GCCAGACAGC AGCCTGCCCA 
GCCCTCCGCC CCGACGGCTC CCTCCCAGGG TTTGACCTGA TGGTGGCCTT CAGCCTGGTG 
GAAAAGGCTT ATGCGTCCAT CCGGGGCGTG GCCATGGAGC CCTCTGCCTT CGGTGGGACC 
CCGACCTTCA CGCTCTTCAA GGACGCCCAG CTGACAAGAC GGGTCAGTGA CGTCCTCCCA 
GCCCCCCTGC CTCCAGAGCA CACCATCGTC TTCCTTCTGC GCCTACTTCC CGAGACACCC 
CGCGAGGCCT TCGCGCTGTG GCAGATGACG GCCGAGGACT TCCAGCCCCT CCTTGGGGTT 
CTGCTGGATG CCGGGAGGAA GTCCCTGACC TACTTCCACC GCGACCCCAG GGCTGCCTTG 
CAGGAGGCCA CCTTCGACCC GCAGGAAGTG AGGAAGATTT TCTTCGGGAG CTTCCACAAG 
GTGCACGTGG CTGTGGGCCG CTCCAAGGTC AGGCTCTATG TGGACTGCCG GAAGGTGGCT 
GAGCGGCCCC TTGGGGAGAT GAGCAGCCCA CCCGCTGCCG GCTTCGTCAC GCTGGGGAGG 
CTGGCCAAAG CCAGGGGCCC CCGGAGCAGT TCGGCCGTGT TTCAGCTCCA GATGCTGCAG 
ATCGTGTGCA GTGACACCTG GGCCGATGAG GACCGGTGCT GTGAGCTCCC TGCCTCGAGG 
GATGGAGAGA CCTGCCCGGC CTTCGTGTCT GCCTGTTCCT GTTCCTCAGA GACCCCTGGG 
CCCCCAGGAC CTCAAGGACC CCCAGGCCTC CCTGGGAGGA ATGGCACCCC AGGAGAGCAG 
GGCTTCCCAG GGCCCAGGGG AGAGCCCGGG CCACCCGGAC AGATGGGACC AGAAGGTCCT 
GGAGGCCAGC AGGGCTCGCC GGGGACCCAG GGCCGCGCAG TCCAGGGGCC TGTGGGTCCA 
CCAGGGGTCA AAGGAGAGAA AGGAGACCAT GGGCTCCCAG GCTTGCAGGG CCACCCCGGC 
CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG 
GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC 
AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG 
CCCAAAGGGG AACGAGGAGA GAAGCTCACG TGTCAAAGTT CGACTCCTTC CACAAGAACA 
CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG CCCCTGGGGT 
CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT GGCCGCCACC 
TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG CAGCAGGGGG 
CTAGCACCCA GGGCCTCTGG GAGTGACAGG ACATTTTCTG CACTGCCCCG AGGAACGCTG 
AGCCTTCCTC CCTGGGTTTC TCTGGACACC GAGAGCGACC ACATCCTGGA GAAGCCAGGA 
GAAAAGCTCA GGAAGAGCCT GCAGGTGGAA GGAGAGAGAA GCAGCGGCCT CGGCCAAGGC 
CCACCCCATG CTCTTGGCTC TGTAGCATTT CCAAGTTCAG ATAAACCCCT GAGTGCTCAC 
CCAGCCAAGC GAGGCGGGAG TCATTTCCTG GGAGAGTGTC CCCCACCCTG GCTCCGTACA 
CCCCACCTTG AGGCCCTGCA GCTTCAGCCG CAGTGGGGAA GGGGAGGGGC TGGGTTGATG 
GCGAATTGGG TTCAGGAGGG CAGCCGTGCC CTGTGGCCAT CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435889.2
CDS	145..4452
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAEGSLELLVRRAALGSRGLAPRASGSDRTFSALPRGTLSLPPWVSLDTESDHILEKPGEKLRKSLQVEGERSSGLGQGPPHALGSVAFPSSDKPLSAHPAKRGGSHFLGECPPPWLRTPHLEALQLQPQWGRGGAGLMANWVQEGSRALWPSQ

Target ORF information:

RefSeq Version	XM_009437614.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437614.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC GGCTCCAGCC 
CTGGACACCC TCCCTGCCCC CACCAGCCTG GTCCTGAGCC AGGTGACCTC CTCCAGCATC 
CGCCTGTCCT GGACTCCAGC CCCCCGGCAC CCCCTCAAGT ATCTGATCGT TTGGCGAGCC 
TCTAGAGGTG GCACCCCCAG GGAGGTGGTG GTGGAGGGGC CCGCCGCCTC CACGGAGCTG 
CACAACCTGG CCTCCCGCAC AGAGTACCTG GTCTCCGTGT TCCCCATCTA TGAGGGCGGG 
GTTGGCGAAG GCCTGCGGGG CCTGGTGACC ACAGCACCTC TGCCTCCGCC CCGGGCGCTG 
ACCCTGGCCG CAGTGACGCC CAGAACCATC CACCTCACCT GGCAGCCCTC GGCCGGGGCC 
ACCCACTACC TGGTGCGATG TTCTCCTGCT TCCCCCAAGG GTGAGGAGGA GGAGCGAGAG 
GTGCAGGTCG GGCGGCCCGA GGTGCTGCTG GATGGCCTGG AACCTGGCAG GGACTATGAG 
GTCTCGGTGC AGAGCCTGCG AGGCCCTGAG GGCAGCGAGG CCCGGGGCAT CCATGCCAGG 
ACCCCCGCCC TGGCCCCCCC GAGACACCTG GGCTTCTCAG ACGTGAGCCA CGACGCGGCA 
CGAGTGTTCT GGGAGGGTGC CCCAAGGCCT GTGCGCCTGG TCAGGGTCAC CTATGTCTCC 
AGCGAGGGTG GACACTCGGG GCAGACAGAG GCTCCTGGGA ACGCCACCTC GGCCACGCTG 
GGGCCTCTCT CTTCCTCCAC CACCTACACT GTCCGTGTCA CCTGCCTCTA CCCTGGGGGT 
GGCTCCTCTA CGCTGACTGG CCGGGTGACC ACCAAGAAAG CTCCCAGCCC AAGCCAGCTG 
TCCGTGACGG AGCTGCCAGG CGACGCAGTC CAGCTGGCGT GGGTGGCCGC AGCCCCGTCT 
GGCGTGCTTG TCTACCAGAT CACATGGACG CCCCTGGGAG AGGGGAAGGC TCACGAGATC 
TCTGTCCCAG GGAACCTCGG CACGGCCGTC CTGCCTGGCC TAGGGAGGCA CACAGAGTAC 
AACGTCACCA TCTTGGCCTA CTACAGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGC 
TATACCCCCT CCACGGTGAG CAGGAGCCCA CCCTCCAACC TGGCCCTGGC CTCGGAGACC 
CCCGACAGCC TGCAGGTCAG CTGGACACCC CCGCTTGGCC ACGTGCTCCA TTACTGGCTC 
ACCTACGCCC CCGCCTCTGG CTTGGGACCT GAGAAATCCG TCTCTGTGCC AGCACCCAGG 
AGCCACGTGA CACTGCCCGA CCTGCAGGCA GCCACGAAGT ACAGGGTCCT GCTCTCAGCT 
ATCTATGCAG CAGGCAGGAG TGAGGCTGTG TCTGCCATGG GCCAGACAGC AGCCTGCCCA 
GCCCTCCGCC CCGACGGCTC CCTCCCAGGG TTTGACCTGA TGGTGGCCTT CAGCCTGGTG 
GAAAAGGCTT ATGCGTCCAT CCGGGGCGTG GCCATGGAGC CCTCTGCCTT CGGTGGGACC 
CCGACCTTCA CGCTCTTCAA GGACGCCCAG CTGACAAGAC GGGTCAGTGA CGTCCTCCCA 
GCCCCCCTGC CTCCAGAGCA CACCATCGTC TTCCTTCTGC GCCTACTTCC CGAGACACCC 
CGCGAGGCCT TCGCGCTGTG GCAGATGACG GCCGAGGACT TCCAGCCCCT CCTTGGGGTT 
CTGCTGGATG CCGGGAGGAA GTCCCTGACC TACTTCCACC GCGACCCCAG GGCTGCCTTG 
CAGGAGGCCA CCTTCGACCC GCAGGAAGTG AGGAAGATTT TCTTCGGGAG CTTCCACAAG 
GTGCACGTGG CTGTGGGCCG CTCCAAGGTC AGGCTCTATG TGGACTGCCG GAAGGTGGCT 
GAGCGGCCCC TTGGGGAGAT GAGCAGCCCA CCCGCTGCCG GCTTCGTCAC GCTGGGGAGG 
CTGGCCAAAG CCAGGGGCCC CCGGAGCAGT TCGGCCGTGT TTCAGCTCCA GATGCTGCAG 
ATCGTGTGCA GTGACACCTG GGCCGATGAG GACCGGTGCT GTGAGCTCCC TGCCTCGAGG 
GATGGAGAGA CCTGCCCGGC CTTCGTGTCT GCCTGTTCCT GTTCCTCAGA GACCCCTGGG 
CCCCCAGGAC CTCAAGGACC CCCAGGCCTC CCTGGGAGGA ATGGCACCCC AGGAGAGCAG 
GGCTTCCCAG GGCCCAGGGG AGAGCCCGGG CCACCCGGAC AGATGGGACC AGAAGGTCCT 
GGAGGCCAGC AGGGCTCGCC GGGGACCCAG GGCCGCGCAG TCCAGGGGCC TGTGGGTCCA 
CCAGGGGTCA AAGGAGAGAA AGGAGACCAT GGGCTCCCAG GCTTGCAGGG CCACCCCGGC 
CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG 
GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC 
AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG 
CCCAAAGGGG AACGAGGAGA GAAGCTCACG TGTCAAAGTT CGACTCCTTC CACAAGAACA 
CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG CCCCTGGGGT 
CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT GGCCGCCACC 
TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG CAGCAGGGGG 
CTAGCACCCA GGGCCTCTGG GAGTGACAGG ACATTTTCTG CACTGCCCCG AGGAACGCTG 
AGCCTTCCTC CCTGGGTTTC TCTGGACACC GAGAGCGACC ACATCCTGGA GAAGCCAGGA 
GAAAAGCTCA GGAAGAGCCT GCAGGTGGAA GGAGAGAGAA GCAGCGGCCT CGGCCAAGGC 
CCACCCCATG CTCTTGGCTC TGTAGCATTT CCAAGTTCAG ATAAACCCCT GAGTGCTCAC 
CCAGCCAAGC GAGGCGGGAG TCATTTCCTG GGAGAGTGTC CCCCACCCTG GCTCCGTACA 
CCCCACCTTG AGGCCCTGCA GCTTCAGCCG CAGTGGGGAA GGGGAGGGGC TGGGTTGATG 
GCGAATTGGG TTCAGGAGGG CAGCCGTGCC CTGTGGCCAT CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437615.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435890.2 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc102061
Clone ID Related Accession (Same CDS sequence)	XM_009437615.3
Accession Version	XM_009437615.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437615.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCCTGCCCA 
GCCCTCCGCC CCGACGGCTC CCTCCCAGGG TTTGACCTGA TGGTGGCCTT CAGCCTGGTG 
GAAAAGGCTT ATGCGTCCAT CCGGGGCGTG GCCATGGAGC CCTCTGCCTT CGGTGGGACC 
CCGACCTTCA CGCTCTTCAA GGACGCCCAG CTGACAAGAC GGGTCAGTGA CGTCCTCCCA 
GCCCCCCTGC CTCCAGAGCA CACCATCGTC TTCCTTCTGC GCCTACTTCC CGAGACACCC 
CGCGAGGCCT TCGCGCTGTG GCAGATGACG GCCGAGGACT TCCAGCCCCT CCTTGGGGTT 
CTGCTGGATG CCGGGAGGAA GTCCCTGACC TACTTCCACC GCGACCCCAG GGCTGCCTTG 
CAGGAGGCCA CCTTCGACCC GCAGGAAGTG AGGAAGATTT TCTTCGGGAG CTTCCACAAG 
GTGCACGTGG CTGTGGGCCG CTCCAAGGTC AGGCTCTATG TGGACTGCCG GAAGGTGGCT 
GAGCGGCCCC TTGGGGAGAT GAGCAGCCCA CCCGCTGCCG GCTTCGTCAC GCTGGGGAGG 
CTGGCCAAAG CCAGGGGCCC CCGGAGCAGT TCGGCCGTGT TTCAGCTCCA GATGCTGCAG 
ATCGTGTGCA GTGACACCTG GGCCGATGAG GACCGGTGCT GTGAGCTCCC TGCCTCGAGG 
GATGGAGAGA CCTGCCCGGC CTTCGTGTCT GCCTGTTCCT GTTCCTCAGA GACCCCTGGG 
CCCCCAGGAC CTCAAGGACC CCCAGGCCTC CCTGGGAGGA ATGGCACCCC AGGAGAGCAG 
GGCTTCCCAG GGCCCAGGGG AGAGCCCGGG CCACCCGGAC AGATGGGACC AGAAGGTCCT 
GGAGGCCAGC AGGGCTCGCC GGGGACCCAG GGCCGCGCAG TCCAGGGGCC TGTGGGTCCA 
CCAGGGGTCA AAGGAGAGAA AGGAGACCAT GGGCTCCCAG GCTTGCAGGG CCACCCCGGC 
CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG 
GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC 
AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG 
CCCAAAGGGG AACGAGGAGA GAAGCTCACG TGTCAAAGTT CGACTCCTTC CACAAGAACA 
CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG CCCCTGGGGT 
CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT GGCCGCCACC 
TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG CAGCAGGGGG 
CTAGCACCCA GGGCCTCTGG GAGTGACAGG ACATTTTCTG CACTGCCCCG AGGAACGCTG 
AGCCTTCCTC CCTGGGTTTC TCTGGACACC GAGAGCGACC ACATCCTGGA GAAGCCAGGA 
GAAAAGCTCA GGAAGAGCCT GCAGGTGGAA GGAGAGAGAA GCAGCGGCCT CGGCCAAGGC 
CCACCCCATG CTCTTGGCTC TGTAGCATTT CCAAGTTCAG ATAAACCCCT GAGTGCTCAC 
CCAGCCAAGC GAGGCGGGAG TCATTTCCTG GGAGAGTGTC CCCCACCCTG GCTCCGTACA 
CCCCACCTTG AGGCCCTGCA GCTTCAGCCG CAGTGGGGAA GGGGAGGGGC TGGGTTGATG 
GCGAATTGGG TTCAGGAGGG CAGCCGTGCC CTGTGGCCAT CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435890.2
CDS	145..4452
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAEGSLELLVRRAALGSRGLAPRASGSDRTFSALPRGTLSLPPWVSLDTESDHILEKPGEKLRKSLQVEGERSSGLGQGPPHALGSVAFPSSDKPLSAHPAKRGGSHFLGECPPPWLRTPHLEALQLQPQWGRGGAGLMANWVQEGSRALWPSQ

Target ORF information:

RefSeq Version	XM_009437615.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437615.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCCTGCCCA 
GCCCTCCGCC CCGACGGCTC CCTCCCAGGG TTTGACCTGA TGGTGGCCTT CAGCCTGGTG 
GAAAAGGCTT ATGCGTCCAT CCGGGGCGTG GCCATGGAGC CCTCTGCCTT CGGTGGGACC 
CCGACCTTCA CGCTCTTCAA GGACGCCCAG CTGACAAGAC GGGTCAGTGA CGTCCTCCCA 
GCCCCCCTGC CTCCAGAGCA CACCATCGTC TTCCTTCTGC GCCTACTTCC CGAGACACCC 
CGCGAGGCCT TCGCGCTGTG GCAGATGACG GCCGAGGACT TCCAGCCCCT CCTTGGGGTT 
CTGCTGGATG CCGGGAGGAA GTCCCTGACC TACTTCCACC GCGACCCCAG GGCTGCCTTG 
CAGGAGGCCA CCTTCGACCC GCAGGAAGTG AGGAAGATTT TCTTCGGGAG CTTCCACAAG 
GTGCACGTGG CTGTGGGCCG CTCCAAGGTC AGGCTCTATG TGGACTGCCG GAAGGTGGCT 
GAGCGGCCCC TTGGGGAGAT GAGCAGCCCA CCCGCTGCCG GCTTCGTCAC GCTGGGGAGG 
CTGGCCAAAG CCAGGGGCCC CCGGAGCAGT TCGGCCGTGT TTCAGCTCCA GATGCTGCAG 
ATCGTGTGCA GTGACACCTG GGCCGATGAG GACCGGTGCT GTGAGCTCCC TGCCTCGAGG 
GATGGAGAGA CCTGCCCGGC CTTCGTGTCT GCCTGTTCCT GTTCCTCAGA GACCCCTGGG 
CCCCCAGGAC CTCAAGGACC CCCAGGCCTC CCTGGGAGGA ATGGCACCCC AGGAGAGCAG 
GGCTTCCCAG GGCCCAGGGG AGAGCCCGGG CCACCCGGAC AGATGGGACC AGAAGGTCCT 
GGAGGCCAGC AGGGCTCGCC GGGGACCCAG GGCCGCGCAG TCCAGGGGCC TGTGGGTCCA 
CCAGGGGTCA AAGGAGAGAA AGGAGACCAT GGGCTCCCAG GCTTGCAGGG CCACCCCGGC 
CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG 
GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC 
AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG 
CCCAAAGGGG AACGAGGAGA GAAGCTCACG TGTCAAAGTT CGACTCCTTC CACAAGAACA 
CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG CCCCTGGGGT 
CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT GGCCGCCACC 
TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG CAGCAGGGGG 
CTAGCACCCA GGGCCTCTGG GAGTGACAGG ACATTTTCTG CACTGCCCCG AGGAACGCTG 
AGCCTTCCTC CCTGGGTTTC TCTGGACACC GAGAGCGACC ACATCCTGGA GAAGCCAGGA 
GAAAAGCTCA GGAAGAGCCT GCAGGTGGAA GGAGAGAGAA GCAGCGGCCT CGGCCAAGGC 
CCACCCCATG CTCTTGGCTC TGTAGCATTT CCAAGTTCAG ATAAACCCCT GAGTGCTCAC 
CCAGCCAAGC GAGGCGGGAG TCATTTCCTG GGAGAGTGTC CCCCACCCTG GCTCCGTACA 
CCCCACCTTG AGGCCCTGCA GCTTCAGCCG CAGTGGGGAA GGGGAGGGGC TGGGTTGATG 
GCGAATTGGG TTCAGGAGGG CAGCCGTGCC CTGTGGCCAT CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437616.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435891.2 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA.

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CloneID	OPc102062
Clone ID Related Accession (Same CDS sequence)	XM_009437616.3
Accession Version	XM_009437616.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437616.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC GGCTCCAGCC 
CTGGACACCC TCCCTGCCCC CACCAGCCTG GTCCTGAGCC AGGTGACCTC CTCCAGCATC 
CGCCTGTCCT GGACTCCAGC CCCCCGGCAC CCCCTCAAGT ATCTGATCGT TTGGCGAGCC 
TCTAGAGGTG GCACCCCCAG GGAGGTGGTG GTGGAGGGGC CCGCCGCCTC CACGGAGCTG 
CACAACCTGG CCTCCCGCAC AGAGTACCTG GTCTCCGTGT TCCCCATCTA TGAGGGCGGG 
GTTGGCGAAG GCCTGCGGGG CCTGGTGACC ACAGCACCTC TGCCTCCGCC CCGGGCGCTG 
ACCCTGGCCG CAGTGACGCC CAGAACCATC CACCTCACCT GGCAGCCCTC GGCCGGGGCC 
ACCCACTACC TGGTGCGATG TTCTCCTGCT TCCCCCAAGG GTGAGGAGGA GGAGCGAGAG 
GTGCAGGTCG GGCGGCCCGA GGTGCTGCTG GATGGCCTGG AACCTGGCAG GGACTATGAG 
GTCTCGGTGC AGAGCCTGCG AGGCCCTGAG GGCAGCGAGG CCCGGGGCAT CCATGCCAGG 
ACCCCCGCCC TGGCCCCCCC GAGACACCTG GGCTTCTCAG ACGTGAGCCA CGACGCGGCA 
CGAGTGTTCT GGGAGGGTGC CCCAAGGCCT GTGCGCCTGG TCAGGGTCAC CTATGTCTCC 
AGCGAGGGTG GACACTCGGG GCAGACAGAG GCTCCTGGGA ACGCCACCTC GGCCACGCTG 
GGGCCTCTCT CTTCCTCCAC CACCTACACT GTCCGTGTCA CCTGCCTCTA CCCTGGGGGT 
GGCTCCTCTA CGCTGACTGG CCGGGTGACC ACCAAGAAAG CTCCCAGCCC AAGCCAGCTG 
TCCGTGACGG AGCTGCCAGG CGACGCAGTC CAGCTGGCGT GGGTGGCCGC AGCCCCGTCT 
GGCGTGCTTG TCTACCAGAT CACATGGACG CCCCTGGGAG AGGGGAAGGC TCACGAGATC 
TCTGTCCCAG GGAACCTCGG CACGGCCGTC CTGCCTGGCC TAGGGAGGCA CACAGAGTAC 
AACGTCACCA TCTTGGCCTA CTACAGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGC 
TATACCCCCT CCACGGTGAG CAGGAGCCCA CCCTCCAACC TGGCCCTGGC CTCGGAGACC 
CCCGACAGCC TGCAGGTCAG CTGGACACCC CCGCTTGGCC ACGTGCTCCA TTACTGGCTC 
ACCTACGCCC CCGCCTCTGG CTTGGGACCT GAGAAATCCG TCTCTGTGCC AGCACCCAGG 
AGCCACGTGA CACTGCCCGA CCTGCAGGCA GCCACGAAGT ACAGGGTCCT GCTCTCAGCT 
ATCTATGCAG CAGGCAGGAG TGAGGCTGTG TCTGCCATGG GCCAGACAGC CTGCCCAGCC 
CTCCGCCCCG ACGGCTCCCT CCCAGGGTTT GACCTGATGG TGGCCTTCAG CCTGGTGGAA 
AAGGCTTATG CGTCCATCCG GGGCGTGGCC ATGGAGCCCT CTGCCTTCGG TGGGACCCCG 
ACCTTCACGC TCTTCAAGGA CGCCCAGCTG ACAAGACGGG TCAGTGACGT CCTCCCAGCC 
CCCCTGCCTC CAGAGCACAC CATCGTCTTC CTTCTGCGCC TACTTCCCGA GACACCCCGC 
GAGGCCTTCG CGCTGTGGCA GATGACGGCC GAGGACTTCC AGCCCCTCCT TGGGGTTCTG 
CTGGATGCCG GGAGGAAGTC CCTGACCTAC TTCCACCGCG ACCCCAGGGC TGCCTTGCAG 
GAGGCCACCT TCGACCCGCA GGAAGTGAGG AAGATTTTCT TCGGGAGCTT CCACAAGGTG 
CACGTGGCTG TGGGCCGCTC CAAGGTCAGG CTCTATGTGG ACTGCCGGAA GGTGGCTGAG 
CGGCCCCTTG GGGAGATGAG CAGCCCACCC GCTGCCGGCT TCGTCACGCT GGGGAGGCTG 
GCCAAAGCCA GGGGCCCCCG GAGCAGTTCG GCCGTGTTTC AGCTCCAGAT GCTGCAGATC 
GTGTGCAGTG ACACCTGGGC CGATGAGGAC CGGTGCTGTG AGCTCCCTGC CTCGAGGGAT 
GGAGAGACCT GCCCGGCCTT CGTGTCTGCC TGTTCCTGTT CCTCAGAGAC CCCTGGGCCC 
CCAGGACCTC AAGGACCCCC AGGCCTCCCT GGGAGGAATG GCACCCCAGG AGAGCAGGGC 
TTCCCAGGGC CCAGGGGAGA GCCCGGGCCA CCCGGACAGA TGGGACCAGA AGGTCCTGGA 
GGCCAGCAGG GCTCGCCGGG GACCCAGGGC CGCGCAGTCC AGGGGCCTGT GGGTCCACCA 
GGGGTCAAAG GAGAGAAAGG AGACCATGGG CTCCCAGGCT TGCAGGGCCA CCCCGGCCAC 
CAGGGCATCC CCGGGAGAGT TGGCCTCCAG GGACCAAAGG GAATGAGAGG CCTGGAGGGA 
ACTGCTGGCC TGCCTGGACC CCCTGGCCCC AGGGGGTTCC AGGGCATGGC AGGGGCCAGG 
GGCACTAGTG GAGAGCGAGG ACCTCCAGGG ACCGTGGGGC CCACAGGACT GCCAGGGCCC 
AAAGGGGAAC GAGGAGAGAA GCTCACGTGT CAAAGTTCGA CTCCTTCCAC AAGAACACCA 
GGCCCCCCAT GCCCATCTTG GAGCAGAAGC TGGAGCCGGG CACTGAGCCC CTGGGGTCCC 
CTGGCACCCG CAGCAAGGCC CTGGTTCCTG GAGAATGGGG GCGTGGTGGC CGCCACCTTG 
AGGGCAGAGG GGAGCCTGGA GCTGTTGGTC AGACGGGCAG CCCTGGGCAG CAGGGGGCTA 
GCACCCAGGG CCTCTGGGAG TGACAGGACA TTTTCTGCAC TGCCCCGAGG AACGCTGAGC 
CTTCCTCCCT GGGTTTCTCT GGACACCGAG AGCGACCACA TCCTGGAGAA GCCAGGAGAA 
AAGCTCAGGA AGAGCCTGCA GGTGGAAGGA GAGAGAAGCA GCGGCCTCGG CCAAGGCCCA 
CCCCATGCTC TTGGCTCTGT AGCATTTCCA AGTTCAGATA AACCCCTGAG TGCTCACCCA 
GCCAAGCGAG GCGGGAGTCA TTTCCTGGGA GAGTGTCCCC CACCCTGGCT CCGTACACCC 
CACCTTGAGG CCCTGCAGCT TCAGCCGCAG TGGGGAAGGG GAGGGGCTGG GTTGATGGCG 
AATTGGGTTC AGGAGGGCAG CCGTGCCCTG TGGCCATCTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435891.2
CDS	145..4449
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAEGSLELLVRRAALGSRGLAPRASGSDRTFSALPRGTLSLPPWVSLDTESDHILEKPGEKLRKSLQVEGERSSGLGQGPPHALGSVAFPSSDKPLSAHPAKRGGSHFLGECPPPWLRTPHLEALQLQPQWGRGGAGLMANWVQEGSRALWPSQ

Target ORF information:

RefSeq Version	XM_009437616.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437616.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC GGCTCCAGCC 
CTGGACACCC TCCCTGCCCC CACCAGCCTG GTCCTGAGCC AGGTGACCTC CTCCAGCATC 
CGCCTGTCCT GGACTCCAGC CCCCCGGCAC CCCCTCAAGT ATCTGATCGT TTGGCGAGCC 
TCTAGAGGTG GCACCCCCAG GGAGGTGGTG GTGGAGGGGC CCGCCGCCTC CACGGAGCTG 
CACAACCTGG CCTCCCGCAC AGAGTACCTG GTCTCCGTGT TCCCCATCTA TGAGGGCGGG 
GTTGGCGAAG GCCTGCGGGG CCTGGTGACC ACAGCACCTC TGCCTCCGCC CCGGGCGCTG 
ACCCTGGCCG CAGTGACGCC CAGAACCATC CACCTCACCT GGCAGCCCTC GGCCGGGGCC 
ACCCACTACC TGGTGCGATG TTCTCCTGCT TCCCCCAAGG GTGAGGAGGA GGAGCGAGAG 
GTGCAGGTCG GGCGGCCCGA GGTGCTGCTG GATGGCCTGG AACCTGGCAG GGACTATGAG 
GTCTCGGTGC AGAGCCTGCG AGGCCCTGAG GGCAGCGAGG CCCGGGGCAT CCATGCCAGG 
ACCCCCGCCC TGGCCCCCCC GAGACACCTG GGCTTCTCAG ACGTGAGCCA CGACGCGGCA 
CGAGTGTTCT GGGAGGGTGC CCCAAGGCCT GTGCGCCTGG TCAGGGTCAC CTATGTCTCC 
AGCGAGGGTG GACACTCGGG GCAGACAGAG GCTCCTGGGA ACGCCACCTC GGCCACGCTG 
GGGCCTCTCT CTTCCTCCAC CACCTACACT GTCCGTGTCA CCTGCCTCTA CCCTGGGGGT 
GGCTCCTCTA CGCTGACTGG CCGGGTGACC ACCAAGAAAG CTCCCAGCCC AAGCCAGCTG 
TCCGTGACGG AGCTGCCAGG CGACGCAGTC CAGCTGGCGT GGGTGGCCGC AGCCCCGTCT 
GGCGTGCTTG TCTACCAGAT CACATGGACG CCCCTGGGAG AGGGGAAGGC TCACGAGATC 
TCTGTCCCAG GGAACCTCGG CACGGCCGTC CTGCCTGGCC TAGGGAGGCA CACAGAGTAC 
AACGTCACCA TCTTGGCCTA CTACAGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGC 
TATACCCCCT CCACGGTGAG CAGGAGCCCA CCCTCCAACC TGGCCCTGGC CTCGGAGACC 
CCCGACAGCC TGCAGGTCAG CTGGACACCC CCGCTTGGCC ACGTGCTCCA TTACTGGCTC 
ACCTACGCCC CCGCCTCTGG CTTGGGACCT GAGAAATCCG TCTCTGTGCC AGCACCCAGG 
AGCCACGTGA CACTGCCCGA CCTGCAGGCA GCCACGAAGT ACAGGGTCCT GCTCTCAGCT 
ATCTATGCAG CAGGCAGGAG TGAGGCTGTG TCTGCCATGG GCCAGACAGC CTGCCCAGCC 
CTCCGCCCCG ACGGCTCCCT CCCAGGGTTT GACCTGATGG TGGCCTTCAG CCTGGTGGAA 
AAGGCTTATG CGTCCATCCG GGGCGTGGCC ATGGAGCCCT CTGCCTTCGG TGGGACCCCG 
ACCTTCACGC TCTTCAAGGA CGCCCAGCTG ACAAGACGGG TCAGTGACGT CCTCCCAGCC 
CCCCTGCCTC CAGAGCACAC CATCGTCTTC CTTCTGCGCC TACTTCCCGA GACACCCCGC 
GAGGCCTTCG CGCTGTGGCA GATGACGGCC GAGGACTTCC AGCCCCTCCT TGGGGTTCTG 
CTGGATGCCG GGAGGAAGTC CCTGACCTAC TTCCACCGCG ACCCCAGGGC TGCCTTGCAG 
GAGGCCACCT TCGACCCGCA GGAAGTGAGG AAGATTTTCT TCGGGAGCTT CCACAAGGTG 
CACGTGGCTG TGGGCCGCTC CAAGGTCAGG CTCTATGTGG ACTGCCGGAA GGTGGCTGAG 
CGGCCCCTTG GGGAGATGAG CAGCCCACCC GCTGCCGGCT TCGTCACGCT GGGGAGGCTG 
GCCAAAGCCA GGGGCCCCCG GAGCAGTTCG GCCGTGTTTC AGCTCCAGAT GCTGCAGATC 
GTGTGCAGTG ACACCTGGGC CGATGAGGAC CGGTGCTGTG AGCTCCCTGC CTCGAGGGAT 
GGAGAGACCT GCCCGGCCTT CGTGTCTGCC TGTTCCTGTT CCTCAGAGAC CCCTGGGCCC 
CCAGGACCTC AAGGACCCCC AGGCCTCCCT GGGAGGAATG GCACCCCAGG AGAGCAGGGC 
TTCCCAGGGC CCAGGGGAGA GCCCGGGCCA CCCGGACAGA TGGGACCAGA AGGTCCTGGA 
GGCCAGCAGG GCTCGCCGGG GACCCAGGGC CGCGCAGTCC AGGGGCCTGT GGGTCCACCA 
GGGGTCAAAG GAGAGAAAGG AGACCATGGG CTCCCAGGCT TGCAGGGCCA CCCCGGCCAC 
CAGGGCATCC CCGGGAGAGT TGGCCTCCAG GGACCAAAGG GAATGAGAGG CCTGGAGGGA 
ACTGCTGGCC TGCCTGGACC CCCTGGCCCC AGGGGGTTCC AGGGCATGGC AGGGGCCAGG 
GGCACTAGTG GAGAGCGAGG ACCTCCAGGG ACCGTGGGGC CCACAGGACT GCCAGGGCCC 
AAAGGGGAAC GAGGAGAGAA GCTCACGTGT CAAAGTTCGA CTCCTTCCAC AAGAACACCA 
GGCCCCCCAT GCCCATCTTG GAGCAGAAGC TGGAGCCGGG CACTGAGCCC CTGGGGTCCC 
CTGGCACCCG CAGCAAGGCC CTGGTTCCTG GAGAATGGGG GCGTGGTGGC CGCCACCTTG 
AGGGCAGAGG GGAGCCTGGA GCTGTTGGTC AGACGGGCAG CCCTGGGCAG CAGGGGGCTA 
GCACCCAGGG CCTCTGGGAG TGACAGGACA TTTTCTGCAC TGCCCCGAGG AACGCTGAGC 
CTTCCTCCCT GGGTTTCTCT GGACACCGAG AGCGACCACA TCCTGGAGAA GCCAGGAGAA 
AAGCTCAGGA AGAGCCTGCA GGTGGAAGGA GAGAGAAGCA GCGGCCTCGG CCAAGGCCCA 
CCCCATGCTC TTGGCTCTGT AGCATTTCCA AGTTCAGATA AACCCCTGAG TGCTCACCCA 
GCCAAGCGAG GCGGGAGTCA TTTCCTGGGA GAGTGTCCCC CACCCTGGCT CCGTACACCC 
CACCTTGAGG CCCTGCAGCT TCAGCCGCAG TGGGGAAGGG GAGGGGCTGG GTTGATGGCG 
AATTGGGTTC AGGAGGGCAG CCGTGCCCTG TGGCCATCTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437620.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435895.2 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X5, mRNA.

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CloneID	OPc102063
Clone ID Related Accession (Same CDS sequence)	XM_009437620.3
Accession Version	XM_009437620.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437620.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGTACTGG GCTCCTGGCT 
CTTGGGGAGG GAGTTGTGGC CGGTGGGGGC GCCGGTTGTC CCCTGGTCCT GGGGCTGGGG 
CTTCAATCTG TGTCAGCACA GCAGCTCCTG CTGGAGATGC CACTGTCCAG CCTCTGGGGT 
GCTGGGGTGA GGACACAAGG CTTCCCTGAG CCCACACAAA GCCAGTCATG GCAAAGAGGT 
CAGCCTCGGC CTGGCCTTCC AGGGCCCTCA AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435895.2
CDS	148..4323
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLHHLPFQGEPQSLATLYQLVSQACESAIQTHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGTGLLALGEGVVAGGGAGCPLVLGLGLQSVSAQQLLLEMPLSSLWGAGVRTQGFPEPTQSQSWQRGQPRPGLPGPSR

Target ORF information:

RefSeq Version	XM_009437620.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437620.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGTACTGG GCTCCTGGCT 
CTTGGGGAGG GAGTTGTGGC CGGTGGGGGC GCCGGTTGTC CCCTGGTCCT GGGGCTGGGG 
CTTCAATCTG TGTCAGCACA GCAGCTCCTG CTGGAGATGC CACTGTCCAG CCTCTGGGGT 
GCTGGGGTGA GGACACAAGG CTTCCCTGAG CCCACACAAA GCCAGTCATG GCAAAGAGGT 
CAGCCTCGGC CTGGCCTTCC AGGGCCCTCA AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437621.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435896.2 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X6, mRNA.

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CloneID	OPc102064
Clone ID Related Accession (Same CDS sequence)	XM_009437621.3
Accession Version	XM_009437621.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437621.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTCTC ACGTGTCAAA GTTCGACTCC 
TTCCACAAGA ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT 
GAGCCCCTGG GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT 
GGTGGCCGCC ACCTTGAGGG CAGAGGTACT GGGCTCCTGG CTCTTGGGGA GGGAGTTGTG 
GCCGGTGGGG GCGCCGGTTG TCCCCTGGTC CTGGGGCTGG GGCTTCAATC TGTGTCAGCA 
CAGCAGCTCC TGCTGGAGAT GCCACTGTCC AGCCTCTGGG GTGCTGGGGT GAGGACACAA 
GGCTTCCCTG AGCCCACACA AAGCCAGTCA TGGCAAAGAG GTCAGCCTCG GCCTGGCCTT 
CCAGGGCCCT CAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435896.2
CDS	149..4306
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLHHLPFQGEPQSLATLYQLVSQASHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGTGLLALGEGVVAGGGAGCPLVLGLGLQSVSAQQLLLEMPLSSLWGAGVRTQGFPEPTQSQSWQRGQPRPGLPGPSR

Target ORF information:

RefSeq Version	XM_009437621.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437621.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTCTC ACGTGTCAAA GTTCGACTCC 
TTCCACAAGA ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT 
GAGCCCCTGG GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT 
GGTGGCCGCC ACCTTGAGGG CAGAGGTACT GGGCTCCTGG CTCTTGGGGA GGGAGTTGTG 
GCCGGTGGGG GCGCCGGTTG TCCCCTGGTC CTGGGGCTGG GGCTTCAATC TGTGTCAGCA 
CAGCAGCTCC TGCTGGAGAT GCCACTGTCC AGCCTCTGGG GTGCTGGGGT GAGGACACAA 
GGCTTCCCTG AGCCCACACA AAGCCAGTCA TGGCAAAGAG GTCAGCCTCG GCCTGGCCTT 
CCAGGGCCCT CAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437622.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435897.2 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPc102065
Clone ID Related Accession (Same CDS sequence)	XM_009437622.3
Accession Version	XM_009437622.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTGTGA GTCTGCCATT CAGACTCACG TGTCAAAGTT CGACTCCTTC 
CACAAGAACA CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG 
CCCCTGGGGT CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT 
GGCCGCCACC TTGAGGGCAG AGGTACTGGG CTCCTGGCTC TTGGGGAGGG AGTTGTGGCC 
GGTGGGGGCG CCGGTTGTCC CCTGGTCCTG GGGCTGGGGC TTCAATCTGT GTCAGCACAG 
CAGCTCCTGC TGGAGATGCC ACTGTCCAGC CTCTGGGGTG CTGGGGTGAG GACACAAGGC 
TTCCCTGAGC CCACACAAAG CCAGTCATGG CAAAGAGGTC AGCCTCGGCC TGGCCTTCCA 
GGGCCCTCAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435897.2
CDS	147..4301
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKGEPQSLATLYQLVSQACESAIQTHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGTGLLALGEGVVAGGGAGCPLVLGLGLQSVSAQQLLLEMPLSSLWGAGVRTQGFPEPTQSQSWQRGQPRPGLPGPSR

Target ORF information:

RefSeq Version	XM_009437622.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437622.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTGTGA GTCTGCCATT CAGACTCACG TGTCAAAGTT CGACTCCTTC 
CACAAGAACA CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG 
CCCCTGGGGT CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT 
GGCCGCCACC TTGAGGGCAG AGGTACTGGG CTCCTGGCTC TTGGGGAGGG AGTTGTGGCC 
GGTGGGGGCG CCGGTTGTCC CCTGGTCCTG GGGCTGGGGC TTCAATCTGT GTCAGCACAG 
CAGCTCCTGC TGGAGATGCC ACTGTCCAGC CTCTGGGGTG CTGGGGTGAG GACACAAGGC 
TTCCCTGAGC CCACACAAAG CCAGTCATGG CAAAGAGGTC AGCCTCGGCC TGGCCTTCCA 
GGGCCCTCAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_016938328.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016793817.1 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X8, mRNA.

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CloneID	OPc102066
Clone ID Related Accession (Same CDS sequence)	XM_016938328.2
Accession Version	XM_016938328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016938328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTCTCA CGTGTCAAAG TTCGACTCCT TCCACAAGAA CACCAGGCCC 
CCCATGCCCA TCTTGGAGCA GAAGCTGGAG CCGGGCACTG AGCCCCTGGG GTCCCCTGGC 
ACCCGCAGCA AGGCCCTGGT TCCTGGAGAA TGGGGGCGTG GTGGCCGCCA CCTTGAGGGC 
AGAGGTACTG GGCTCCTGGC TCTTGGGGAG GGAGTTGTGG CCGGTGGGGG CGCCGGTTGT 
CCCCTGGTCC TGGGGCTGGG GCTTCAATCT GTGTCAGCAC AGCAGCTCCT GCTGGAGATG 
CCACTGTCCA GCCTCTGGGG TGCTGGGGTG AGGACACAAG GCTTCCCTGA GCCCACACAA 
AGCCAGTCAT GGCAAAGAGG TCAGCCTCGG CCTGGCCTTC CAGGGCCCTC AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016793817.1
CDS	148..4284
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKGEPQSLATLYQLVSQASHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGTGLLALGEGVVAGGGAGCPLVLGLGLQSVSAQQLLLEMPLSSLWGAGVRTQGFPEPTQSQSWQRGQPRPGLPGPSR

Target ORF information:

RefSeq Version	XM_016938328.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016938328.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTCTCA CGTGTCAAAG TTCGACTCCT TCCACAAGAA CACCAGGCCC 
CCCATGCCCA TCTTGGAGCA GAAGCTGGAG CCGGGCACTG AGCCCCTGGG GTCCCCTGGC 
ACCCGCAGCA AGGCCCTGGT TCCTGGAGAA TGGGGGCGTG GTGGCCGCCA CCTTGAGGGC 
AGAGGTACTG GGCTCCTGGC TCTTGGGGAG GGAGTTGTGG CCGGTGGGGG CGCCGGTTGT 
CCCCTGGTCC TGGGGCTGGG GCTTCAATCT GTGTCAGCAC AGCAGCTCCT GCTGGAGATG 
CCACTGTCCA GCCTCTGGGG TGCTGGGGTG AGGACACAAG GCTTCCCTGA GCCCACACAA 
AGCCAGTCAT GGCAAAGAGG TCAGCCTCGG CCTGGCCTTC CAGGGCCCTC AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_016938329.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016793818.1 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X9, mRNA.

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CloneID	OPc102067
Clone ID Related Accession (Same CDS sequence)	XM_016938329.2
Accession Version	XM_016938329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016938329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGACGTCTC AGGAAACTGG 
GGGCAACCTG GGGGGAGCCC CGTGGGCTGT GGGAACCCCT GCCCCCGGCT TGGAAGCTGC 
CCACGGTGGC CTGGTTGCAG CCTGGCAGAT GAGGGGTACA TCACTACTCA GAATAAACGG 
CCCCCTCTGC ACCCACCTGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016793818.1
CDS	147..4250
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLHHLPFQGEPQSLATLYQLVSQACESAIQTHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRDVSGNWGQPGGSPVGCGNPCPRLGSCPRWPGCSLADEGYITTQNKRPPLHPPV

Target ORF information:

RefSeq Version	XM_016938329.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016938329.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGACGTCTC AGGAAACTGG 
GGGCAACCTG GGGGGAGCCC CGTGGGCTGT GGGAACCCCT GCCCCCGGCT TGGAAGCTGC 
CCACGGTGGC CTGGTTGCAG CCTGGCAGAT GAGGGGTACA TCACTACTCA GAATAAACGG 
CCCCCTCTGC ACCCACCTGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437623.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435898.1 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OPc102068
Clone ID Related Accession (Same CDS sequence)	XM_009437623.3
Accession Version	XM_009437623.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437623.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
GTCTCTGTGC CAGCACCCAG GAGCCACGTG ACACTGCCCG ACCTGCAGGC AGCCACGAAG 
TACAGGGTCC TGCTCTCAGC TATCTATGCA GCAGGCAGGA GTGAGGCTGT GTCTGCCATG 
GGCCAGACAG CAGCCTGCCC AGCCCTCCGC CCCGACGGCT CCCTCCCAGG GTTTGACCTG 
ATGGTGGCCT TCAGCCTGGT GGAAAAGGCT TATGCGTCCA TCCGGGGCGT GGCCATGGAG 
CCCTCTGCCT TCGGTGGGAC CCCGACCTTC ACGCTCTTCA AGGACGCCCA GCTGACAAGA 
CGGGTCAGTG ACGTCCTCCC AGCCCCCCTG CCTCCAGAGC ACACCATCGT CTTCCTTCTG 
CGCCTACTTC CCGAGACACC CCGCGAGGCC TTCGCGCTGT GGCAGATGAC GGCCGAGGAC 
TTCCAGCCCC TCCTTGGGGT TCTGCTGGAT GCCGGGAGGA AGTCCCTGAC CTACTTCCAC 
CGCGACCCCA GGGCTGCCTT GCAGGAGGCC ACCTTCGACC CGCAGGAAGT GAGGAAGATT 
TTCTTCGGGA GCTTCCACAA GGTGCACGTG GCTGTGGGCC GCTCCAAGGT CAGGCTCTAT 
GTGGACTGCC GGAAGGTGGC TGAGCGGCCC CTTGGGGAGA TGAGCAGCCC ACCCGCTGCC 
GGCTTCGTCA CGCTGGGGAG GCTGGCCAAA GCCAGGGGCC CCCGGAGCAG TTCGGCCGTG 
TTTCAGCTCC AGATGCTGCA GATCGTGTGC AGTGACACCT GGGCCGATGA GGACCGGTGC 
TGTGAGCTCC CTGCCTCGAG GGATGGAGAG ACCTGCCCGG CCTTCGTGTC TGCCTGTTCC 
TGTTCCTCAG AGACCCCTGG GCCCCCAGGA CCTCAAGGAC CCCCAGGCCT CCCTGGGAGG 
AATGGCACCC CAGGAGAGCA GGGCTTCCCA GGGCCCAGGG GAGAGCCCGG GCCACCCGGA 
CAGATGGGAC CAGAAGGTCC TGGAGGCCAG CAGGGCTCGC CGGGGACCCA GGGCCGCGCA 
GTCCAGGGGC CTGTGGGTCC ACCAGGGGTC AAAGGAGAGA AAGGAGACCA TGGGCTCCCA 
GGCTTGCAGG GCCACCCCGG CCACCAGGGC ATCCCCGGGA GAGTTGGCCT CCAGGGACCA 
AAGGGAATGA GAGGCCTGGA GGGAACTGCT GGCCTGCCTG GACCCCCTGG CCCCAGGGGG 
TTCCAGGGCA TGGCAGGGGC CAGGGGCACT AGTGGAGAGC GAGGACCTCC AGGGACCGTG 
GGGCCCACAG GACTGCCAGG GCCCAAAGGG GAACGAGGAG AGAAGCTCAC GTGTCAAAGT 
TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG AAGCTGGAGC 
CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT CCTGGAGAAT 
GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGGGAGCC TGGAGCTGTT GGTCAGACGG 
GCAGCCCTGG GCAGCAGGGG GCTAGCACCC AGGGCCTCTG GGAGTGACAG GACATTTTCT 
GCACTGCCCC GAGGAACGCT GAGCCTTCCT CCCTGGGTTT CTCTGGACAC CGAGAGCGAC 
CACATCCTGG AGAAGCCAGG AGAAAAGCTC AGGAAGAGCC TGCAGGTGGA AGGAGAGAGA 
AGCAGCGGCC TCGGCCAAGG CCCACCCCAT GCTCTTGGCT CTGTAGCATT TCCAAGTTCA 
GATAAACCCC TGAGTGCTCA CCCAGCCAAG CGAGGCGGGA GTCATTTCCT GGGAGAGTGT 
CCCCCACCCT GGCTCCGTAC ACCCCACCTT GAGGCCCTGC AGCTTCAGCC GCAGTGGGGA 
AGGGGAGGGG CTGGGTTGAT GGCGAATTGG GTTCAGGAGG GCAGCCGTGC CCTGTGGCCA 
TCTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435898.1
CDS	145..4173
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAEGSLELLVRRAALGSRGLAPRASGSDRTFSALPRGTLSLPPWVSLDTESDHILEKPGEKLRKSLQVEGERSSGLGQGPPHALGSVAFPSSDKPLSAHPAKRGGSHFLGECPPPWLRTPHLEALQLQPQWGRGGAGLMANWVQEGSRALWPSQ

Target ORF information:

RefSeq Version	XM_009437623.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437623.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
GTCTCTGTGC CAGCACCCAG GAGCCACGTG ACACTGCCCG ACCTGCAGGC AGCCACGAAG 
TACAGGGTCC TGCTCTCAGC TATCTATGCA GCAGGCAGGA GTGAGGCTGT GTCTGCCATG 
GGCCAGACAG CAGCCTGCCC AGCCCTCCGC CCCGACGGCT CCCTCCCAGG GTTTGACCTG 
ATGGTGGCCT TCAGCCTGGT GGAAAAGGCT TATGCGTCCA TCCGGGGCGT GGCCATGGAG 
CCCTCTGCCT TCGGTGGGAC CCCGACCTTC ACGCTCTTCA AGGACGCCCA GCTGACAAGA 
CGGGTCAGTG ACGTCCTCCC AGCCCCCCTG CCTCCAGAGC ACACCATCGT CTTCCTTCTG 
CGCCTACTTC CCGAGACACC CCGCGAGGCC TTCGCGCTGT GGCAGATGAC GGCCGAGGAC 
TTCCAGCCCC TCCTTGGGGT TCTGCTGGAT GCCGGGAGGA AGTCCCTGAC CTACTTCCAC 
CGCGACCCCA GGGCTGCCTT GCAGGAGGCC ACCTTCGACC CGCAGGAAGT GAGGAAGATT 
TTCTTCGGGA GCTTCCACAA GGTGCACGTG GCTGTGGGCC GCTCCAAGGT CAGGCTCTAT 
GTGGACTGCC GGAAGGTGGC TGAGCGGCCC CTTGGGGAGA TGAGCAGCCC ACCCGCTGCC 
GGCTTCGTCA CGCTGGGGAG GCTGGCCAAA GCCAGGGGCC CCCGGAGCAG TTCGGCCGTG 
TTTCAGCTCC AGATGCTGCA GATCGTGTGC AGTGACACCT GGGCCGATGA GGACCGGTGC 
TGTGAGCTCC CTGCCTCGAG GGATGGAGAG ACCTGCCCGG CCTTCGTGTC TGCCTGTTCC 
TGTTCCTCAG AGACCCCTGG GCCCCCAGGA CCTCAAGGAC CCCCAGGCCT CCCTGGGAGG 
AATGGCACCC CAGGAGAGCA GGGCTTCCCA GGGCCCAGGG GAGAGCCCGG GCCACCCGGA 
CAGATGGGAC CAGAAGGTCC TGGAGGCCAG CAGGGCTCGC CGGGGACCCA GGGCCGCGCA 
GTCCAGGGGC CTGTGGGTCC ACCAGGGGTC AAAGGAGAGA AAGGAGACCA TGGGCTCCCA 
GGCTTGCAGG GCCACCCCGG CCACCAGGGC ATCCCCGGGA GAGTTGGCCT CCAGGGACCA 
AAGGGAATGA GAGGCCTGGA GGGAACTGCT GGCCTGCCTG GACCCCCTGG CCCCAGGGGG 
TTCCAGGGCA TGGCAGGGGC CAGGGGCACT AGTGGAGAGC GAGGACCTCC AGGGACCGTG 
GGGCCCACAG GACTGCCAGG GCCCAAAGGG GAACGAGGAG AGAAGCTCAC GTGTCAAAGT 
TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG AAGCTGGAGC 
CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT CCTGGAGAAT 
GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGGGAGCC TGGAGCTGTT GGTCAGACGG 
GCAGCCCTGG GCAGCAGGGG GCTAGCACCC AGGGCCTCTG GGAGTGACAG GACATTTTCT 
GCACTGCCCC GAGGAACGCT GAGCCTTCCT CCCTGGGTTT CTCTGGACAC CGAGAGCGAC 
CACATCCTGG AGAAGCCAGG AGAAAAGCTC AGGAAGAGCC TGCAGGTGGA AGGAGAGAGA 
AGCAGCGGCC TCGGCCAAGG CCCACCCCAT GCTCTTGGCT CTGTAGCATT TCCAAGTTCA 
GATAAACCCC TGAGTGCTCA CCCAGCCAAG CGAGGCGGGA GTCATTTCCT GGGAGAGTGT 
CCCCCACCCT GGCTCCGTAC ACCCCACCTT GAGGCCCTGC AGCTTCAGCC GCAGTGGGGA 
AGGGGAGGGG CTGGGTTGAT GGCGAATTGG GTTCAGGAGG GCAGCCGTGC CCTGTGGCCA 
TCTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_016938330.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016793819.1 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X11, mRNA.

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CloneID	OPc102069
Clone ID Related Accession (Same CDS sequence)	XM_016938330.2
Accession Version	XM_016938330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016938330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTC ACGTGTCAAA GTTCGACTCC TTCCACAAGA 
ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT GAGCCCCTGG 
GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT GGTGGCCGCC 
ACCTTGAGGG CAGAGACGTC TCAGGAAACT GGGGGCAACC TGGGGGGAGC CCCGTGGGCT 
GTGGGAACCC CTGCCCCCGG CTTGGAAGCT GCCCACGGTG GCCTGGTTGC AGCCTGGCAG 
ATGAGGGGTA CATCACTACT CAGAATAAAC GGCCCCCTCT GCACCCACCT GTCTAGACGA 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016793819.1
CDS	148..4173
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAETSQETGGNLGGAPWAVGTPAPGLEAAHGGLVAAWQMRGTSLLRINGPLCTHLSRRG

Target ORF information:

RefSeq Version	XM_016938330.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016938330.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTC ACGTGTCAAA GTTCGACTCC TTCCACAAGA 
ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT GAGCCCCTGG 
GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT GGTGGCCGCC 
ACCTTGAGGG CAGAGACGTC TCAGGAAACT GGGGGCAACC TGGGGGGAGC CCCGTGGGCT 
GTGGGAACCC CTGCCCCCGG CTTGGAAGCT GCCCACGGTG GCCTGGTTGC AGCCTGGCAG 
ATGAGGGGTA CATCACTACT CAGAATAAAC GGCCCCCTCT GCACCCACCT GTCTAGACGA 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437624.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435899.2 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X12, mRNA.

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CloneID	OPc102070
Clone ID Related Accession (Same CDS sequence)	XM_009437624.3
Accession Version	XM_009437624.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437624.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGGGAGCC TGGAGCTGTT 
GGTCAGACGG GCAGCCCTGG GCAGCAGGGG GCTAGCACCC AGGGCCTCTG GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435899.2
CDS	146..4162
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLHHLPFQGEPQSLATLYQLVSQACESAIQTHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGEPGAVGQTGSPGQQGASTQGLWE

Target ORF information:

RefSeq Version	XM_009437624.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437624.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGGGAGCC TGGAGCTGTT 
GGTCAGACGG GCAGCCCTGG GCAGCAGGGG GCTAGCACCC AGGGCCTCTG GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437625.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435900.2 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OPc102071
Clone ID Related Accession (Same CDS sequence)	XM_009437625.3
Accession Version	XM_009437625.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437625.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTGTGA GTCTGCCATT CAGACTCACG TGTCAAAGTT CGACTCCTTC 
CACAAGAACA CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG 
CCCCTGGGGT CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT 
GGCCGCCACC TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG 
CAGCAGGGGG CTAGCACCCA GGGCCTCTGG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435900.2
CDS	145..4140
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKGEPQSLATLYQLVSQACESAIQTHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGEPGAVGQTGSPGQQGASTQGLWE

Target ORF information:

RefSeq Version	XM_009437625.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437625.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTGTGA GTCTGCCATT CAGACTCACG TGTCAAAGTT CGACTCCTTC 
CACAAGAACA CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG 
CCCCTGGGGT CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT 
GGCCGCCACC TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG 
CAGCAGGGGG CTAGCACCCA GGGCCTCTGG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437626.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435901.2 Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X14, mRNA.

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CloneID	OPc102072
Clone ID Related Accession (Same CDS sequence)	XM_009437626.3
Accession Version	XM_009437626.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036899.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009437626.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTCTCA CGTGTCAAAG TTCGACTCCT TCCACAAGAA CACCAGGCCC 
CCCATGCCCA TCTTGGAGCA GAAGCTGGAG CCGGGCACTG AGCCCCTGGG GTCCCCTGGC 
ACCCGCAGCA AGGCCCTGGT TCCTGGAGAA TGGGGGCGTG GTGGCCGCCA CCTTGAGGGC 
AGAGGGGAGC CTGGAGCTGT TGGTCAGACG GGCAGCCCTG GGCAGCAGGG GGCTAGCACC 
CAGGGCCTCT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435901.2
CDS	146..4123
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKGEPQSLATLYQLVSQASHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGEPGAVGQTGSPGQQGASTQGLWE

Target ORF information:

RefSeq Version	XM_009437626.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 chain (COL20A1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437626.3

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AAGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTCTCA CGTGTCAAAG TTCGACTCCT TCCACAAGAA CACCAGGCCC 
CCCATGCCCA TCTTGGAGCA GAAGCTGGAG CCGGGCACTG AGCCCCTGGG GTCCCCTGGC 
ACCCGCAGCA AGGCCCTGGT TCCTGGAGAA TGGGGGCGTG GTGGCCGCCA CCTTGAGGGC 
AGAGGGGAGC CTGGAGCTGT TGGTCAGACG GGCAGCCCTG GGCAGCAGGG GGCTAGCACC 
CAGGGCCTCT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437613.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435888.2 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X1, mRNA.

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CloneID	OPc77414
Clone ID Related Accession (Same CDS sequence)	XM_009437613.2
Accession Version	XM_009437613.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437613.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTC ACGTGTCAAA GTTCGACTCC TTCCACAAGA 
ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT GAGCCCCTGG 
GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT GGTGGCCGCC 
ACCTTGAGGG CAGAGGGGAG CCTGGAGCTG TTGGTCAGAC GGGCAGCCCT GGGCAGCAGG 
GGGCTAGCAC CCAGGGCCTC TGGGAGTGAC AGGACATTTT CTGCACTGCC CCGAGGAACG 
CTGAGCCTTC CTCCCTGGGT TTCTCTGGAC ACCGAGAGCG ACCACATCCT GGAGAAGCCA 
GGAGAAAAGC TCAGGAAGAG CCTGCAGGTG GAAGGAGAGA GAAGCAGCGG CCTCGGCCAA 
GGCCCACCCC ATGCTCTTGG CTCTGTAGCA TTTCCAAGTT CAGATAAACC CCTGAGTGCT 
CACCCAGCCA AGCGAGGCGG GAGTCATTTC CTGGGAGAGT GTCCCCCACC CTGGCTCCGT 
ACACCCCACC TTGAGGCCCT GCAGCTTCAG CCGCAGTGGG GAAGGGGAGG GGCTGGGTTG 
ATGGCGAATT GGGTTCAGGA GGGCAGCCGT GCCCTGTGGC CATCTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435888.2
CDS	145..4455
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAEGSLELLVRRAALGSRGLAPRASGSDRTFSALPRGTLSLPPWVSLDTESDHILEKPGEKLRKSLQVEGERSSGLGQGPPHALGSVAFPSSDKPLSAHPAKRGGSHFLGECPPPWLRTPHLEALQLQPQWGRGGAGLMANWVQEGSRALWPSQ

Target ORF information:

RefSeq Version	XM_009437613.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437613.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTC ACGTGTCAAA GTTCGACTCC TTCCACAAGA 
ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT GAGCCCCTGG 
GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT GGTGGCCGCC 
ACCTTGAGGG CAGAGGGGAG CCTGGAGCTG TTGGTCAGAC GGGCAGCCCT GGGCAGCAGG 
GGGCTAGCAC CCAGGGCCTC TGGGAGTGAC AGGACATTTT CTGCACTGCC CCGAGGAACG 
CTGAGCCTTC CTCCCTGGGT TTCTCTGGAC ACCGAGAGCG ACCACATCCT GGAGAAGCCA 
GGAGAAAAGC TCAGGAAGAG CCTGCAGGTG GAAGGAGAGA GAAGCAGCGG CCTCGGCCAA 
GGCCCACCCC ATGCTCTTGG CTCTGTAGCA TTTCCAAGTT CAGATAAACC CCTGAGTGCT 
CACCCAGCCA AGCGAGGCGG GAGTCATTTC CTGGGAGAGT GTCCCCCACC CTGGCTCCGT 
ACACCCCACC TTGAGGCCCT GCAGCTTCAG CCGCAGTGGG GAAGGGGAGG GGCTGGGTTG 
ATGGCGAATT GGGTTCAGGA GGGCAGCCGT GCCCTGTGGC CATCTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437614.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435889.2 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc77415
Clone ID Related Accession (Same CDS sequence)	XM_009437614.2
Accession Version	XM_009437614.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437614.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC GGCTCCAGCC 
CTGGACACCC TCCCTGCCCC CACCAGCCTG GTCCTGAGCC AGGTGACCTC CTCCAGCATC 
CGCCTGTCCT GGACTCCAGC CCCCCGGCAC CCCCTCAAGT ATCTGATCGT TTGGCGAGCC 
TCTAGAGGTG GCACCCCCAG GGAGGTGGTG GTGGAGGGGC CCGCCGCCTC CACGGAGCTG 
CACAACCTGG CCTCCCGCAC AGAGTACCTG GTCTCCGTGT TCCCCATCTA TGAGGGCGGG 
GTTGGCGAAG GCCTGCGGGG CCTGGTGACC ACAGCACCTC TGCCTCCGCC CCGGGCGCTG 
ACCCTGGCCG CAGTGACGCC CAGAACCATC CACCTCACCT GGCAGCCCTC GGCCGGGGCC 
ACCCACTACC TGGTGCGATG TTCTCCTGCT TCCCCCAAGG GTGAGGAGGA GGAGCGAGAG 
GTGCAGGTCG GGCGGCCCGA GGTGCTGCTG GATGGCCTGG AACCTGGCAG GGACTATGAG 
GTCTCGGTGC AGAGCCTGCG AGGCCCTGAG GGCAGCGAGG CCCGGGGCAT CCATGCCAGG 
ACCCCCGCCC TGGCCCCCCC GAGACACCTG GGCTTCTCAG ACGTGAGCCA CGACGCGGCA 
CGAGTGTTCT GGGAGGGTGC CCCAAGGCCT GTGCGCCTGG TCAGGGTCAC CTATGTCTCC 
AGCGAGGGTG GACACTCGGG GCAGACAGAG GCTCCTGGGA ACGCCACCTC GGCCACGCTG 
GGGCCTCTCT CTTCCTCCAC CACCTACACT GTCCGTGTCA CCTGCCTCTA CCCTGGGGGT 
GGCTCCTCTA CGCTGACTGG CCGGGTGACC ACCAAGAAAG CTCCCAGCCC AAGCCAGCTG 
TCCGTGACGG AGCTGCCAGG CGACGCAGTC CAGCTGGCGT GGGTGGCCGC AGCCCCGTCT 
GGCGTGCTTG TCTACCAGAT CACATGGACG CCCCTGGGAG AGGGGAAGGC TCACGAGATC 
TCTGTCCCAG GGAACCTCGG CACGGCCGTC CTGCCTGGCC TAGGGAGGCA CACAGAGTAC 
AACGTCACCA TCTTGGCCTA CTACAGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGC 
TATACCCCCT CCACGGTGAG CAGGAGCCCA CCCTCCAACC TGGCCCTGGC CTCGGAGACC 
CCCGACAGCC TGCAGGTCAG CTGGACACCC CCGCTTGGCC ACGTGCTCCA TTACTGGCTC 
ACCTACGCCC CCGCCTCTGG CTTGGGACCT GAGAAATCCG TCTCTGTGCC AGCACCCAGG 
AGCCACGTGA CACTGCCCGA CCTGCAGGCA GCCACGAAGT ACAGGGTCCT GCTCTCAGCT 
ATCTATGCAG CAGGCAGGAG TGAGGCTGTG TCTGCCATGG GCCAGACAGC AGCCTGCCCA 
GCCCTCCGCC CCGACGGCTC CCTCCCAGGG TTTGACCTGA TGGTGGCCTT CAGCCTGGTG 
GAAAAGGCTT ATGCGTCCAT CCGGGGCGTG GCCATGGAGC CCTCTGCCTT CGGTGGGACC 
CCGACCTTCA CGCTCTTCAA GGACGCCCAG CTGACAAGAC GGGTCAGTGA CGTCCTCCCA 
GCCCCCCTGC CTCCAGAGCA CACCATCGTC TTCCTTCTGC GCCTACTTCC CGAGACACCC 
CGCGAGGCCT TCGCGCTGTG GCAGATGACG GCCGAGGACT TCCAGCCCCT CCTTGGGGTT 
CTGCTGGATG CCGGGAGGAA GTCCCTGACC TACTTCCACC GCGACCCCAG GGCTGCCTTG 
CAGGAGGCCA CCTTCGACCC GCAGGAAGTG AGGAAGATTT TCTTCGGGAG CTTCCACAAG 
GTGCACGTGG CTGTGGGCCG CTCCAAGGTC AGGCTCTATG TGGACTGCCG GAAGGTGGCT 
GAGCGGCCCC TTGGGGAGAT GAGCAGCCCA CCCGCTGCCG GCTTCGTCAC GCTGGGGAGG 
CTGGCCAAGG CCAGGGGCCC CCGGAGCAGT TCGGCCGTGT TTCAGCTCCA GATGCTGCAG 
ATCGTGTGCA GTGACACCTG GGCCGATGAG GACCGGTGCT GTGAGCTCCC TGCCTCGAGG 
GATGGAGAGA CCTGCCCGGC CTTCGTGTCT GCCTGTTCCT GTTCCTCAGA GACCCCTGGG 
CCCCCAGGAC CTCAAGGACC CCCAGGCCTC CCTGGGAGGA ATGGCACCCC AGGAGAGCAG 
GGCTTCCCAG GGCCCAGGGG AGAGCCCGGG CCACCCGGAC AGATGGGACC AGAAGGTCCT 
GGAGGCCAGC AGGGCTCGCC GGGGACCCAG GGCCGCGCAG TCCAGGGGCC TGTGGGTCCA 
CCAGGGGTCA AAGGAGAGAA AGGAGACCAT GGGCTCCCAG GCTTGCAGGG CCACCCCGGC 
CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG 
GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC 
AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG 
CCCAAAGGGG AACGAGGAGA GAAGCTCACG TGTCAAAGTT CGACTCCTTC CACAAGAACA 
CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG CCCCTGGGGT 
CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT GGCCGCCACC 
TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG CAGCAGGGGG 
CTAGCACCCA GGGCCTCTGG GAGTGACAGG ACATTTTCTG CACTGCCCCG AGGAACGCTG 
AGCCTTCCTC CCTGGGTTTC TCTGGACACC GAGAGCGACC ACATCCTGGA GAAGCCAGGA 
GAAAAGCTCA GGAAGAGCCT GCAGGTGGAA GGAGAGAGAA GCAGCGGCCT CGGCCAAGGC 
CCACCCCATG CTCTTGGCTC TGTAGCATTT CCAAGTTCAG ATAAACCCCT GAGTGCTCAC 
CCAGCCAAGC GAGGCGGGAG TCATTTCCTG GGAGAGTGTC CCCCACCCTG GCTCCGTACA 
CCCCACCTTG AGGCCCTGCA GCTTCAGCCG CAGTGGGGAA GGGGAGGGGC TGGGTTGATG 
GCGAATTGGG TTCAGGAGGG CAGCCGTGCC CTGTGGCCAT CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435889.2
CDS	144..4451
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAEGSLELLVRRAALGSRGLAPRASGSDRTFSALPRGTLSLPPWVSLDTESDHILEKPGEKLRKSLQVEGERSSGLGQGPPHALGSVAFPSSDKPLSAHPAKRGGSHFLGECPPPWLRTPHLEALQLQPQWGRGGAGLMANWVQEGSRALWPSQ

Target ORF information:

RefSeq Version	XM_009437614.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437614.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC GGCTCCAGCC 
CTGGACACCC TCCCTGCCCC CACCAGCCTG GTCCTGAGCC AGGTGACCTC CTCCAGCATC 
CGCCTGTCCT GGACTCCAGC CCCCCGGCAC CCCCTCAAGT ATCTGATCGT TTGGCGAGCC 
TCTAGAGGTG GCACCCCCAG GGAGGTGGTG GTGGAGGGGC CCGCCGCCTC CACGGAGCTG 
CACAACCTGG CCTCCCGCAC AGAGTACCTG GTCTCCGTGT TCCCCATCTA TGAGGGCGGG 
GTTGGCGAAG GCCTGCGGGG CCTGGTGACC ACAGCACCTC TGCCTCCGCC CCGGGCGCTG 
ACCCTGGCCG CAGTGACGCC CAGAACCATC CACCTCACCT GGCAGCCCTC GGCCGGGGCC 
ACCCACTACC TGGTGCGATG TTCTCCTGCT TCCCCCAAGG GTGAGGAGGA GGAGCGAGAG 
GTGCAGGTCG GGCGGCCCGA GGTGCTGCTG GATGGCCTGG AACCTGGCAG GGACTATGAG 
GTCTCGGTGC AGAGCCTGCG AGGCCCTGAG GGCAGCGAGG CCCGGGGCAT CCATGCCAGG 
ACCCCCGCCC TGGCCCCCCC GAGACACCTG GGCTTCTCAG ACGTGAGCCA CGACGCGGCA 
CGAGTGTTCT GGGAGGGTGC CCCAAGGCCT GTGCGCCTGG TCAGGGTCAC CTATGTCTCC 
AGCGAGGGTG GACACTCGGG GCAGACAGAG GCTCCTGGGA ACGCCACCTC GGCCACGCTG 
GGGCCTCTCT CTTCCTCCAC CACCTACACT GTCCGTGTCA CCTGCCTCTA CCCTGGGGGT 
GGCTCCTCTA CGCTGACTGG CCGGGTGACC ACCAAGAAAG CTCCCAGCCC AAGCCAGCTG 
TCCGTGACGG AGCTGCCAGG CGACGCAGTC CAGCTGGCGT GGGTGGCCGC AGCCCCGTCT 
GGCGTGCTTG TCTACCAGAT CACATGGACG CCCCTGGGAG AGGGGAAGGC TCACGAGATC 
TCTGTCCCAG GGAACCTCGG CACGGCCGTC CTGCCTGGCC TAGGGAGGCA CACAGAGTAC 
AACGTCACCA TCTTGGCCTA CTACAGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGC 
TATACCCCCT CCACGGTGAG CAGGAGCCCA CCCTCCAACC TGGCCCTGGC CTCGGAGACC 
CCCGACAGCC TGCAGGTCAG CTGGACACCC CCGCTTGGCC ACGTGCTCCA TTACTGGCTC 
ACCTACGCCC CCGCCTCTGG CTTGGGACCT GAGAAATCCG TCTCTGTGCC AGCACCCAGG 
AGCCACGTGA CACTGCCCGA CCTGCAGGCA GCCACGAAGT ACAGGGTCCT GCTCTCAGCT 
ATCTATGCAG CAGGCAGGAG TGAGGCTGTG TCTGCCATGG GCCAGACAGC AGCCTGCCCA 
GCCCTCCGCC CCGACGGCTC CCTCCCAGGG TTTGACCTGA TGGTGGCCTT CAGCCTGGTG 
GAAAAGGCTT ATGCGTCCAT CCGGGGCGTG GCCATGGAGC CCTCTGCCTT CGGTGGGACC 
CCGACCTTCA CGCTCTTCAA GGACGCCCAG CTGACAAGAC GGGTCAGTGA CGTCCTCCCA 
GCCCCCCTGC CTCCAGAGCA CACCATCGTC TTCCTTCTGC GCCTACTTCC CGAGACACCC 
CGCGAGGCCT TCGCGCTGTG GCAGATGACG GCCGAGGACT TCCAGCCCCT CCTTGGGGTT 
CTGCTGGATG CCGGGAGGAA GTCCCTGACC TACTTCCACC GCGACCCCAG GGCTGCCTTG 
CAGGAGGCCA CCTTCGACCC GCAGGAAGTG AGGAAGATTT TCTTCGGGAG CTTCCACAAG 
GTGCACGTGG CTGTGGGCCG CTCCAAGGTC AGGCTCTATG TGGACTGCCG GAAGGTGGCT 
GAGCGGCCCC TTGGGGAGAT GAGCAGCCCA CCCGCTGCCG GCTTCGTCAC GCTGGGGAGG 
CTGGCCAAGG CCAGGGGCCC CCGGAGCAGT TCGGCCGTGT TTCAGCTCCA GATGCTGCAG 
ATCGTGTGCA GTGACACCTG GGCCGATGAG GACCGGTGCT GTGAGCTCCC TGCCTCGAGG 
GATGGAGAGA CCTGCCCGGC CTTCGTGTCT GCCTGTTCCT GTTCCTCAGA GACCCCTGGG 
CCCCCAGGAC CTCAAGGACC CCCAGGCCTC CCTGGGAGGA ATGGCACCCC AGGAGAGCAG 
GGCTTCCCAG GGCCCAGGGG AGAGCCCGGG CCACCCGGAC AGATGGGACC AGAAGGTCCT 
GGAGGCCAGC AGGGCTCGCC GGGGACCCAG GGCCGCGCAG TCCAGGGGCC TGTGGGTCCA 
CCAGGGGTCA AAGGAGAGAA AGGAGACCAT GGGCTCCCAG GCTTGCAGGG CCACCCCGGC 
CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG 
GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC 
AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG 
CCCAAAGGGG AACGAGGAGA GAAGCTCACG TGTCAAAGTT CGACTCCTTC CACAAGAACA 
CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG CCCCTGGGGT 
CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT GGCCGCCACC 
TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG CAGCAGGGGG 
CTAGCACCCA GGGCCTCTGG GAGTGACAGG ACATTTTCTG CACTGCCCCG AGGAACGCTG 
AGCCTTCCTC CCTGGGTTTC TCTGGACACC GAGAGCGACC ACATCCTGGA GAAGCCAGGA 
GAAAAGCTCA GGAAGAGCCT GCAGGTGGAA GGAGAGAGAA GCAGCGGCCT CGGCCAAGGC 
CCACCCCATG CTCTTGGCTC TGTAGCATTT CCAAGTTCAG ATAAACCCCT GAGTGCTCAC 
CCAGCCAAGC GAGGCGGGAG TCATTTCCTG GGAGAGTGTC CCCCACCCTG GCTCCGTACA 
CCCCACCTTG AGGCCCTGCA GCTTCAGCCG CAGTGGGGAA GGGGAGGGGC TGGGTTGATG 
GCGAATTGGG TTCAGGAGGG CAGCCGTGCC CTGTGGCCAT CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437615.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435890.2 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X3, mRNA.

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CloneID	OPc77416
Clone ID Related Accession (Same CDS sequence)	XM_009437615.2
Accession Version	XM_009437615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437615.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCCTGCCCA 
GCCCTCCGCC CCGACGGCTC CCTCCCAGGG TTTGACCTGA TGGTGGCCTT CAGCCTGGTG 
GAAAAGGCTT ATGCGTCCAT CCGGGGCGTG GCCATGGAGC CCTCTGCCTT CGGTGGGACC 
CCGACCTTCA CGCTCTTCAA GGACGCCCAG CTGACAAGAC GGGTCAGTGA CGTCCTCCCA 
GCCCCCCTGC CTCCAGAGCA CACCATCGTC TTCCTTCTGC GCCTACTTCC CGAGACACCC 
CGCGAGGCCT TCGCGCTGTG GCAGATGACG GCCGAGGACT TCCAGCCCCT CCTTGGGGTT 
CTGCTGGATG CCGGGAGGAA GTCCCTGACC TACTTCCACC GCGACCCCAG GGCTGCCTTG 
CAGGAGGCCA CCTTCGACCC GCAGGAAGTG AGGAAGATTT TCTTCGGGAG CTTCCACAAG 
GTGCACGTGG CTGTGGGCCG CTCCAAGGTC AGGCTCTATG TGGACTGCCG GAAGGTGGCT 
GAGCGGCCCC TTGGGGAGAT GAGCAGCCCA CCCGCTGCCG GCTTCGTCAC GCTGGGGAGG 
CTGGCCAAGG CCAGGGGCCC CCGGAGCAGT TCGGCCGTGT TTCAGCTCCA GATGCTGCAG 
ATCGTGTGCA GTGACACCTG GGCCGATGAG GACCGGTGCT GTGAGCTCCC TGCCTCGAGG 
GATGGAGAGA CCTGCCCGGC CTTCGTGTCT GCCTGTTCCT GTTCCTCAGA GACCCCTGGG 
CCCCCAGGAC CTCAAGGACC CCCAGGCCTC CCTGGGAGGA ATGGCACCCC AGGAGAGCAG 
GGCTTCCCAG GGCCCAGGGG AGAGCCCGGG CCACCCGGAC AGATGGGACC AGAAGGTCCT 
GGAGGCCAGC AGGGCTCGCC GGGGACCCAG GGCCGCGCAG TCCAGGGGCC TGTGGGTCCA 
CCAGGGGTCA AAGGAGAGAA AGGAGACCAT GGGCTCCCAG GCTTGCAGGG CCACCCCGGC 
CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG 
GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC 
AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG 
CCCAAAGGGG AACGAGGAGA GAAGCTCACG TGTCAAAGTT CGACTCCTTC CACAAGAACA 
CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG CCCCTGGGGT 
CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT GGCCGCCACC 
TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG CAGCAGGGGG 
CTAGCACCCA GGGCCTCTGG GAGTGACAGG ACATTTTCTG CACTGCCCCG AGGAACGCTG 
AGCCTTCCTC CCTGGGTTTC TCTGGACACC GAGAGCGACC ACATCCTGGA GAAGCCAGGA 
GAAAAGCTCA GGAAGAGCCT GCAGGTGGAA GGAGAGAGAA GCAGCGGCCT CGGCCAAGGC 
CCACCCCATG CTCTTGGCTC TGTAGCATTT CCAAGTTCAG ATAAACCCCT GAGTGCTCAC 
CCAGCCAAGC GAGGCGGGAG TCATTTCCTG GGAGAGTGTC CCCCACCCTG GCTCCGTACA 
CCCCACCTTG AGGCCCTGCA GCTTCAGCCG CAGTGGGGAA GGGGAGGGGC TGGGTTGATG 
GCGAATTGGG TTCAGGAGGG CAGCCGTGCC CTGTGGCCAT CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435890.2
CDS	145..4452
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAEGSLELLVRRAALGSRGLAPRASGSDRTFSALPRGTLSLPPWVSLDTESDHILEKPGEKLRKSLQVEGERSSGLGQGPPHALGSVAFPSSDKPLSAHPAKRGGSHFLGECPPPWLRTPHLEALQLQPQWGRGGAGLMANWVQEGSRALWPSQ

Target ORF information:

RefSeq Version	XM_009437615.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437615.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCCTGCCCA 
GCCCTCCGCC CCGACGGCTC CCTCCCAGGG TTTGACCTGA TGGTGGCCTT CAGCCTGGTG 
GAAAAGGCTT ATGCGTCCAT CCGGGGCGTG GCCATGGAGC CCTCTGCCTT CGGTGGGACC 
CCGACCTTCA CGCTCTTCAA GGACGCCCAG CTGACAAGAC GGGTCAGTGA CGTCCTCCCA 
GCCCCCCTGC CTCCAGAGCA CACCATCGTC TTCCTTCTGC GCCTACTTCC CGAGACACCC 
CGCGAGGCCT TCGCGCTGTG GCAGATGACG GCCGAGGACT TCCAGCCCCT CCTTGGGGTT 
CTGCTGGATG CCGGGAGGAA GTCCCTGACC TACTTCCACC GCGACCCCAG GGCTGCCTTG 
CAGGAGGCCA CCTTCGACCC GCAGGAAGTG AGGAAGATTT TCTTCGGGAG CTTCCACAAG 
GTGCACGTGG CTGTGGGCCG CTCCAAGGTC AGGCTCTATG TGGACTGCCG GAAGGTGGCT 
GAGCGGCCCC TTGGGGAGAT GAGCAGCCCA CCCGCTGCCG GCTTCGTCAC GCTGGGGAGG 
CTGGCCAAGG CCAGGGGCCC CCGGAGCAGT TCGGCCGTGT TTCAGCTCCA GATGCTGCAG 
ATCGTGTGCA GTGACACCTG GGCCGATGAG GACCGGTGCT GTGAGCTCCC TGCCTCGAGG 
GATGGAGAGA CCTGCCCGGC CTTCGTGTCT GCCTGTTCCT GTTCCTCAGA GACCCCTGGG 
CCCCCAGGAC CTCAAGGACC CCCAGGCCTC CCTGGGAGGA ATGGCACCCC AGGAGAGCAG 
GGCTTCCCAG GGCCCAGGGG AGAGCCCGGG CCACCCGGAC AGATGGGACC AGAAGGTCCT 
GGAGGCCAGC AGGGCTCGCC GGGGACCCAG GGCCGCGCAG TCCAGGGGCC TGTGGGTCCA 
CCAGGGGTCA AAGGAGAGAA AGGAGACCAT GGGCTCCCAG GCTTGCAGGG CCACCCCGGC 
CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG 
GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC 
AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG 
CCCAAAGGGG AACGAGGAGA GAAGCTCACG TGTCAAAGTT CGACTCCTTC CACAAGAACA 
CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG CCCCTGGGGT 
CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT GGCCGCCACC 
TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG CAGCAGGGGG 
CTAGCACCCA GGGCCTCTGG GAGTGACAGG ACATTTTCTG CACTGCCCCG AGGAACGCTG 
AGCCTTCCTC CCTGGGTTTC TCTGGACACC GAGAGCGACC ACATCCTGGA GAAGCCAGGA 
GAAAAGCTCA GGAAGAGCCT GCAGGTGGAA GGAGAGAGAA GCAGCGGCCT CGGCCAAGGC 
CCACCCCATG CTCTTGGCTC TGTAGCATTT CCAAGTTCAG ATAAACCCCT GAGTGCTCAC 
CCAGCCAAGC GAGGCGGGAG TCATTTCCTG GGAGAGTGTC CCCCACCCTG GCTCCGTACA 
CCCCACCTTG AGGCCCTGCA GCTTCAGCCG CAGTGGGGAA GGGGAGGGGC TGGGTTGATG 
GCGAATTGGG TTCAGGAGGG CAGCCGTGCC CTGTGGCCAT CTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437616.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435891.2 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X4, mRNA.

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CloneID	OPc77417
Clone ID Related Accession (Same CDS sequence)	XM_009437616.2
Accession Version	XM_009437616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437616.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC GGCTCCAGCC 
CTGGACACCC TCCCTGCCCC CACCAGCCTG GTCCTGAGCC AGGTGACCTC CTCCAGCATC 
CGCCTGTCCT GGACTCCAGC CCCCCGGCAC CCCCTCAAGT ATCTGATCGT TTGGCGAGCC 
TCTAGAGGTG GCACCCCCAG GGAGGTGGTG GTGGAGGGGC CCGCCGCCTC CACGGAGCTG 
CACAACCTGG CCTCCCGCAC AGAGTACCTG GTCTCCGTGT TCCCCATCTA TGAGGGCGGG 
GTTGGCGAAG GCCTGCGGGG CCTGGTGACC ACAGCACCTC TGCCTCCGCC CCGGGCGCTG 
ACCCTGGCCG CAGTGACGCC CAGAACCATC CACCTCACCT GGCAGCCCTC GGCCGGGGCC 
ACCCACTACC TGGTGCGATG TTCTCCTGCT TCCCCCAAGG GTGAGGAGGA GGAGCGAGAG 
GTGCAGGTCG GGCGGCCCGA GGTGCTGCTG GATGGCCTGG AACCTGGCAG GGACTATGAG 
GTCTCGGTGC AGAGCCTGCG AGGCCCTGAG GGCAGCGAGG CCCGGGGCAT CCATGCCAGG 
ACCCCCGCCC TGGCCCCCCC GAGACACCTG GGCTTCTCAG ACGTGAGCCA CGACGCGGCA 
CGAGTGTTCT GGGAGGGTGC CCCAAGGCCT GTGCGCCTGG TCAGGGTCAC CTATGTCTCC 
AGCGAGGGTG GACACTCGGG GCAGACAGAG GCTCCTGGGA ACGCCACCTC GGCCACGCTG 
GGGCCTCTCT CTTCCTCCAC CACCTACACT GTCCGTGTCA CCTGCCTCTA CCCTGGGGGT 
GGCTCCTCTA CGCTGACTGG CCGGGTGACC ACCAAGAAAG CTCCCAGCCC AAGCCAGCTG 
TCCGTGACGG AGCTGCCAGG CGACGCAGTC CAGCTGGCGT GGGTGGCCGC AGCCCCGTCT 
GGCGTGCTTG TCTACCAGAT CACATGGACG CCCCTGGGAG AGGGGAAGGC TCACGAGATC 
TCTGTCCCAG GGAACCTCGG CACGGCCGTC CTGCCTGGCC TAGGGAGGCA CACAGAGTAC 
AACGTCACCA TCTTGGCCTA CTACAGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGC 
TATACCCCCT CCACGGTGAG CAGGAGCCCA CCCTCCAACC TGGCCCTGGC CTCGGAGACC 
CCCGACAGCC TGCAGGTCAG CTGGACACCC CCGCTTGGCC ACGTGCTCCA TTACTGGCTC 
ACCTACGCCC CCGCCTCTGG CTTGGGACCT GAGAAATCCG TCTCTGTGCC AGCACCCAGG 
AGCCACGTGA CACTGCCCGA CCTGCAGGCA GCCACGAAGT ACAGGGTCCT GCTCTCAGCT 
ATCTATGCAG CAGGCAGGAG TGAGGCTGTG TCTGCCATGG GCCAGACAGC CTGCCCAGCC 
CTCCGCCCCG ACGGCTCCCT CCCAGGGTTT GACCTGATGG TGGCCTTCAG CCTGGTGGAA 
AAGGCTTATG CGTCCATCCG GGGCGTGGCC ATGGAGCCCT CTGCCTTCGG TGGGACCCCG 
ACCTTCACGC TCTTCAAGGA CGCCCAGCTG ACAAGACGGG TCAGTGACGT CCTCCCAGCC 
CCCCTGCCTC CAGAGCACAC CATCGTCTTC CTTCTGCGCC TACTTCCCGA GACACCCCGC 
GAGGCCTTCG CGCTGTGGCA GATGACGGCC GAGGACTTCC AGCCCCTCCT TGGGGTTCTG 
CTGGATGCCG GGAGGAAGTC CCTGACCTAC TTCCACCGCG ACCCCAGGGC TGCCTTGCAG 
GAGGCCACCT TCGACCCGCA GGAAGTGAGG AAGATTTTCT TCGGGAGCTT CCACAAGGTG 
CACGTGGCTG TGGGCCGCTC CAAGGTCAGG CTCTATGTGG ACTGCCGGAA GGTGGCTGAG 
CGGCCCCTTG GGGAGATGAG CAGCCCACCC GCTGCCGGCT TCGTCACGCT GGGGAGGCTG 
GCCAAGGCCA GGGGCCCCCG GAGCAGTTCG GCCGTGTTTC AGCTCCAGAT GCTGCAGATC 
GTGTGCAGTG ACACCTGGGC CGATGAGGAC CGGTGCTGTG AGCTCCCTGC CTCGAGGGAT 
GGAGAGACCT GCCCGGCCTT CGTGTCTGCC TGTTCCTGTT CCTCAGAGAC CCCTGGGCCC 
CCAGGACCTC AAGGACCCCC AGGCCTCCCT GGGAGGAATG GCACCCCAGG AGAGCAGGGC 
TTCCCAGGGC CCAGGGGAGA GCCCGGGCCA CCCGGACAGA TGGGACCAGA AGGTCCTGGA 
GGCCAGCAGG GCTCGCCGGG GACCCAGGGC CGCGCAGTCC AGGGGCCTGT GGGTCCACCA 
GGGGTCAAAG GAGAGAAAGG AGACCATGGG CTCCCAGGCT TGCAGGGCCA CCCCGGCCAC 
CAGGGCATCC CCGGGAGAGT TGGCCTCCAG GGACCAAAGG GAATGAGAGG CCTGGAGGGA 
ACTGCTGGCC TGCCTGGACC CCCTGGCCCC AGGGGGTTCC AGGGCATGGC AGGGGCCAGG 
GGCACTAGTG GAGAGCGAGG ACCTCCAGGG ACCGTGGGGC CCACAGGACT GCCAGGGCCC 
AAAGGGGAAC GAGGAGAGAA GCTCACGTGT CAAAGTTCGA CTCCTTCCAC AAGAACACCA 
GGCCCCCCAT GCCCATCTTG GAGCAGAAGC TGGAGCCGGG CACTGAGCCC CTGGGGTCCC 
CTGGCACCCG CAGCAAGGCC CTGGTTCCTG GAGAATGGGG GCGTGGTGGC CGCCACCTTG 
AGGGCAGAGG GGAGCCTGGA GCTGTTGGTC AGACGGGCAG CCCTGGGCAG CAGGGGGCTA 
GCACCCAGGG CCTCTGGGAG TGACAGGACA TTTTCTGCAC TGCCCCGAGG AACGCTGAGC 
CTTCCTCCCT GGGTTTCTCT GGACACCGAG AGCGACCACA TCCTGGAGAA GCCAGGAGAA 
AAGCTCAGGA AGAGCCTGCA GGTGGAAGGA GAGAGAAGCA GCGGCCTCGG CCAAGGCCCA 
CCCCATGCTC TTGGCTCTGT AGCATTTCCA AGTTCAGATA AACCCCTGAG TGCTCACCCA 
GCCAAGCGAG GCGGGAGTCA TTTCCTGGGA GAGTGTCCCC CACCCTGGCT CCGTACACCC 
CACCTTGAGG CCCTGCAGCT TCAGCCGCAG TGGGGAAGGG GAGGGGCTGG GTTGATGGCG 
AATTGGGTTC AGGAGGGCAG CCGTGCCCTG TGGCCATCTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435891.2
CDS	145..4449
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAEGSLELLVRRAALGSRGLAPRASGSDRTFSALPRGTLSLPPWVSLDTESDHILEKPGEKLRKSLQVEGERSSGLGQGPPHALGSVAFPSSDKPLSAHPAKRGGSHFLGECPPPWLRTPHLEALQLQPQWGRGGAGLMANWVQEGSRALWPSQ

Target ORF information:

RefSeq Version	XM_009437616.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437616.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC GGCTCCAGCC 
CTGGACACCC TCCCTGCCCC CACCAGCCTG GTCCTGAGCC AGGTGACCTC CTCCAGCATC 
CGCCTGTCCT GGACTCCAGC CCCCCGGCAC CCCCTCAAGT ATCTGATCGT TTGGCGAGCC 
TCTAGAGGTG GCACCCCCAG GGAGGTGGTG GTGGAGGGGC CCGCCGCCTC CACGGAGCTG 
CACAACCTGG CCTCCCGCAC AGAGTACCTG GTCTCCGTGT TCCCCATCTA TGAGGGCGGG 
GTTGGCGAAG GCCTGCGGGG CCTGGTGACC ACAGCACCTC TGCCTCCGCC CCGGGCGCTG 
ACCCTGGCCG CAGTGACGCC CAGAACCATC CACCTCACCT GGCAGCCCTC GGCCGGGGCC 
ACCCACTACC TGGTGCGATG TTCTCCTGCT TCCCCCAAGG GTGAGGAGGA GGAGCGAGAG 
GTGCAGGTCG GGCGGCCCGA GGTGCTGCTG GATGGCCTGG AACCTGGCAG GGACTATGAG 
GTCTCGGTGC AGAGCCTGCG AGGCCCTGAG GGCAGCGAGG CCCGGGGCAT CCATGCCAGG 
ACCCCCGCCC TGGCCCCCCC GAGACACCTG GGCTTCTCAG ACGTGAGCCA CGACGCGGCA 
CGAGTGTTCT GGGAGGGTGC CCCAAGGCCT GTGCGCCTGG TCAGGGTCAC CTATGTCTCC 
AGCGAGGGTG GACACTCGGG GCAGACAGAG GCTCCTGGGA ACGCCACCTC GGCCACGCTG 
GGGCCTCTCT CTTCCTCCAC CACCTACACT GTCCGTGTCA CCTGCCTCTA CCCTGGGGGT 
GGCTCCTCTA CGCTGACTGG CCGGGTGACC ACCAAGAAAG CTCCCAGCCC AAGCCAGCTG 
TCCGTGACGG AGCTGCCAGG CGACGCAGTC CAGCTGGCGT GGGTGGCCGC AGCCCCGTCT 
GGCGTGCTTG TCTACCAGAT CACATGGACG CCCCTGGGAG AGGGGAAGGC TCACGAGATC 
TCTGTCCCAG GGAACCTCGG CACGGCCGTC CTGCCTGGCC TAGGGAGGCA CACAGAGTAC 
AACGTCACCA TCTTGGCCTA CTACAGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGC 
TATACCCCCT CCACGGTGAG CAGGAGCCCA CCCTCCAACC TGGCCCTGGC CTCGGAGACC 
CCCGACAGCC TGCAGGTCAG CTGGACACCC CCGCTTGGCC ACGTGCTCCA TTACTGGCTC 
ACCTACGCCC CCGCCTCTGG CTTGGGACCT GAGAAATCCG TCTCTGTGCC AGCACCCAGG 
AGCCACGTGA CACTGCCCGA CCTGCAGGCA GCCACGAAGT ACAGGGTCCT GCTCTCAGCT 
ATCTATGCAG CAGGCAGGAG TGAGGCTGTG TCTGCCATGG GCCAGACAGC CTGCCCAGCC 
CTCCGCCCCG ACGGCTCCCT CCCAGGGTTT GACCTGATGG TGGCCTTCAG CCTGGTGGAA 
AAGGCTTATG CGTCCATCCG GGGCGTGGCC ATGGAGCCCT CTGCCTTCGG TGGGACCCCG 
ACCTTCACGC TCTTCAAGGA CGCCCAGCTG ACAAGACGGG TCAGTGACGT CCTCCCAGCC 
CCCCTGCCTC CAGAGCACAC CATCGTCTTC CTTCTGCGCC TACTTCCCGA GACACCCCGC 
GAGGCCTTCG CGCTGTGGCA GATGACGGCC GAGGACTTCC AGCCCCTCCT TGGGGTTCTG 
CTGGATGCCG GGAGGAAGTC CCTGACCTAC TTCCACCGCG ACCCCAGGGC TGCCTTGCAG 
GAGGCCACCT TCGACCCGCA GGAAGTGAGG AAGATTTTCT TCGGGAGCTT CCACAAGGTG 
CACGTGGCTG TGGGCCGCTC CAAGGTCAGG CTCTATGTGG ACTGCCGGAA GGTGGCTGAG 
CGGCCCCTTG GGGAGATGAG CAGCCCACCC GCTGCCGGCT TCGTCACGCT GGGGAGGCTG 
GCCAAGGCCA GGGGCCCCCG GAGCAGTTCG GCCGTGTTTC AGCTCCAGAT GCTGCAGATC 
GTGTGCAGTG ACACCTGGGC CGATGAGGAC CGGTGCTGTG AGCTCCCTGC CTCGAGGGAT 
GGAGAGACCT GCCCGGCCTT CGTGTCTGCC TGTTCCTGTT CCTCAGAGAC CCCTGGGCCC 
CCAGGACCTC AAGGACCCCC AGGCCTCCCT GGGAGGAATG GCACCCCAGG AGAGCAGGGC 
TTCCCAGGGC CCAGGGGAGA GCCCGGGCCA CCCGGACAGA TGGGACCAGA AGGTCCTGGA 
GGCCAGCAGG GCTCGCCGGG GACCCAGGGC CGCGCAGTCC AGGGGCCTGT GGGTCCACCA 
GGGGTCAAAG GAGAGAAAGG AGACCATGGG CTCCCAGGCT TGCAGGGCCA CCCCGGCCAC 
CAGGGCATCC CCGGGAGAGT TGGCCTCCAG GGACCAAAGG GAATGAGAGG CCTGGAGGGA 
ACTGCTGGCC TGCCTGGACC CCCTGGCCCC AGGGGGTTCC AGGGCATGGC AGGGGCCAGG 
GGCACTAGTG GAGAGCGAGG ACCTCCAGGG ACCGTGGGGC CCACAGGACT GCCAGGGCCC 
AAAGGGGAAC GAGGAGAGAA GCTCACGTGT CAAAGTTCGA CTCCTTCCAC AAGAACACCA 
GGCCCCCCAT GCCCATCTTG GAGCAGAAGC TGGAGCCGGG CACTGAGCCC CTGGGGTCCC 
CTGGCACCCG CAGCAAGGCC CTGGTTCCTG GAGAATGGGG GCGTGGTGGC CGCCACCTTG 
AGGGCAGAGG GGAGCCTGGA GCTGTTGGTC AGACGGGCAG CCCTGGGCAG CAGGGGGCTA 
GCACCCAGGG CCTCTGGGAG TGACAGGACA TTTTCTGCAC TGCCCCGAGG AACGCTGAGC 
CTTCCTCCCT GGGTTTCTCT GGACACCGAG AGCGACCACA TCCTGGAGAA GCCAGGAGAA 
AAGCTCAGGA AGAGCCTGCA GGTGGAAGGA GAGAGAAGCA GCGGCCTCGG CCAAGGCCCA 
CCCCATGCTC TTGGCTCTGT AGCATTTCCA AGTTCAGATA AACCCCTGAG TGCTCACCCA 
GCCAAGCGAG GCGGGAGTCA TTTCCTGGGA GAGTGTCCCC CACCCTGGCT CCGTACACCC 
CACCTTGAGG CCCTGCAGCT TCAGCCGCAG TGGGGAAGGG GAGGGGCTGG GTTGATGGCG 
AATTGGGTTC AGGAGGGCAG CCGTGCCCTG TGGCCATCTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437620.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435895.2 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X5, mRNA.

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CloneID	OPc77418
Clone ID Related Accession (Same CDS sequence)	XM_009437620.2
Accession Version	XM_009437620.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437620.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGTACTGG GCTCCTGGCT 
CTTGGGGAGG GAGTTGTGGC CGGTGGGGGC GCCGGTTGTC CCCTGGTCCT GGGGCTGGGG 
CTTCAATCTG TGTCAGCACA GCAGCTCCTG CTGGAGATGC CACTGTCCAG CCTCTGGGGT 
GCTGGGGTGA GGACACAAGG CTTCCCTGAG CCCACACAAA GCCAGTCATG GCAAAGAGGT 
CAGCCTCGGC CTGGCCTTCC AGGGCCCTCA AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435895.2
CDS	146..4321
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLHHLPFQGEPQSLATLYQLVSQACESAIQTHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGTGLLALGEGVVAGGGAGCPLVLGLGLQSVSAQQLLLEMPLSSLWGAGVRTQGFPEPTQSQSWQRGQPRPGLPGPSR

Target ORF information:

RefSeq Version	XM_009437620.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437620.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGTACTGG GCTCCTGGCT 
CTTGGGGAGG GAGTTGTGGC CGGTGGGGGC GCCGGTTGTC CCCTGGTCCT GGGGCTGGGG 
CTTCAATCTG TGTCAGCACA GCAGCTCCTG CTGGAGATGC CACTGTCCAG CCTCTGGGGT 
GCTGGGGTGA GGACACAAGG CTTCCCTGAG CCCACACAAA GCCAGTCATG GCAAAGAGGT 
CAGCCTCGGC CTGGCCTTCC AGGGCCCTCA AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437621.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435896.2 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPc77419
Clone ID Related Accession (Same CDS sequence)	XM_009437621.2
Accession Version	XM_009437621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTCTC ACGTGTCAAA GTTCGACTCC 
TTCCACAAGA ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT 
GAGCCCCTGG GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT 
GGTGGCCGCC ACCTTGAGGG CAGAGGTACT GGGCTCCTGG CTCTTGGGGA GGGAGTTGTG 
GCCGGTGGGG GCGCCGGTTG TCCCCTGGTC CTGGGGCTGG GGCTTCAATC TGTGTCAGCA 
CAGCAGCTCC TGCTGGAGAT GCCACTGTCC AGCCTCTGGG GTGCTGGGGT GAGGACACAA 
GGCTTCCCTG AGCCCACACA AAGCCAGTCA TGGCAAAGAG GTCAGCCTCG GCCTGGCCTT 
CCAGGGCCCT CAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435896.2
CDS	147..4304
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLHHLPFQGEPQSLATLYQLVSQASHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGTGLLALGEGVVAGGGAGCPLVLGLGLQSVSAQQLLLEMPLSSLWGAGVRTQGFPEPTQSQSWQRGQPRPGLPGPSR

Target ORF information:

RefSeq Version	XM_009437621.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437621.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTCTC ACGTGTCAAA GTTCGACTCC 
TTCCACAAGA ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT 
GAGCCCCTGG GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT 
GGTGGCCGCC ACCTTGAGGG CAGAGGTACT GGGCTCCTGG CTCTTGGGGA GGGAGTTGTG 
GCCGGTGGGG GCGCCGGTTG TCCCCTGGTC CTGGGGCTGG GGCTTCAATC TGTGTCAGCA 
CAGCAGCTCC TGCTGGAGAT GCCACTGTCC AGCCTCTGGG GTGCTGGGGT GAGGACACAA 
GGCTTCCCTG AGCCCACACA AAGCCAGTCA TGGCAAAGAG GTCAGCCTCG GCCTGGCCTT 
CCAGGGCCCT CAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437622.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435897.2 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X7, mRNA.

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CloneID	OPc77420
Clone ID Related Accession (Same CDS sequence)	XM_009437622.2
Accession Version	XM_009437622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTGTGA GTCTGCCATT CAGACTCACG TGTCAAAGTT CGACTCCTTC 
CACAAGAACA CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG 
CCCCTGGGGT CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT 
GGCCGCCACC TTGAGGGCAG AGGTACTGGG CTCCTGGCTC TTGGGGAGGG AGTTGTGGCC 
GGTGGGGGCG CCGGTTGTCC CCTGGTCCTG GGGCTGGGGC TTCAATCTGT GTCAGCACAG 
CAGCTCCTGC TGGAGATGCC ACTGTCCAGC CTCTGGGGTG CTGGGGTGAG GACACAAGGC 
TTCCCTGAGC CCACACAAAG CCAGTCATGG CAAAGAGGTC AGCCTCGGCC TGGCCTTCCA 
GGGCCCTCAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435897.2
CDS	146..4300
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKGEPQSLATLYQLVSQACESAIQTHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGTGLLALGEGVVAGGGAGCPLVLGLGLQSVSAQQLLLEMPLSSLWGAGVRTQGFPEPTQSQSWQRGQPRPGLPGPSR

Target ORF information:

RefSeq Version	XM_009437622.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437622.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTGTGA GTCTGCCATT CAGACTCACG TGTCAAAGTT CGACTCCTTC 
CACAAGAACA CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG 
CCCCTGGGGT CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT 
GGCCGCCACC TTGAGGGCAG AGGTACTGGG CTCCTGGCTC TTGGGGAGGG AGTTGTGGCC 
GGTGGGGGCG CCGGTTGTCC CCTGGTCCTG GGGCTGGGGC TTCAATCTGT GTCAGCACAG 
CAGCTCCTGC TGGAGATGCC ACTGTCCAGC CTCTGGGGTG CTGGGGTGAG GACACAAGGC 
TTCCCTGAGC CCACACAAAG CCAGTCATGG CAAAGAGGTC AGCCTCGGCC TGGCCTTCCA 
GGGCCCTCAA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_016938328.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016793817.1 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X8, mRNA.

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CloneID	OPc77421
Clone ID Related Accession (Same CDS sequence)	XM_016938328.1
Accession Version	XM_016938328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTCTCA CGTGTCAAAG TTCGACTCCT TCCACAAGAA CACCAGGCCC 
CCCATGCCCA TCTTGGAGCA GAAGCTGGAG CCGGGCACTG AGCCCCTGGG GTCCCCTGGC 
ACCCGCAGCA AGGCCCTGGT TCCTGGAGAA TGGGGGCGTG GTGGCCGCCA CCTTGAGGGC 
AGAGGTACTG GGCTCCTGGC TCTTGGGGAG GGAGTTGTGG CCGGTGGGGG CGCCGGTTGT 
CCCCTGGTCC TGGGGCTGGG GCTTCAATCT GTGTCAGCAC AGCAGCTCCT GCTGGAGATG 
CCACTGTCCA GCCTCTGGGG TGCTGGGGTG AGGACACAAG GCTTCCCTGA GCCCACACAA 
AGCCAGTCAT GGCAAAGAGG TCAGCCTCGG CCTGGCCTTC CAGGGCCCTC AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016793817.1
CDS	147..4283
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKGEPQSLATLYQLVSQASHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGTGLLALGEGVVAGGGAGCPLVLGLGLQSVSAQQLLLEMPLSSLWGAGVRTQGFPEPTQSQSWQRGQPRPGLPGPSR

Target ORF information:

RefSeq Version	XM_016938328.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016938328.1

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTCTCA CGTGTCAAAG TTCGACTCCT TCCACAAGAA CACCAGGCCC 
CCCATGCCCA TCTTGGAGCA GAAGCTGGAG CCGGGCACTG AGCCCCTGGG GTCCCCTGGC 
ACCCGCAGCA AGGCCCTGGT TCCTGGAGAA TGGGGGCGTG GTGGCCGCCA CCTTGAGGGC 
AGAGGTACTG GGCTCCTGGC TCTTGGGGAG GGAGTTGTGG CCGGTGGGGG CGCCGGTTGT 
CCCCTGGTCC TGGGGCTGGG GCTTCAATCT GTGTCAGCAC AGCAGCTCCT GCTGGAGATG 
CCACTGTCCA GCCTCTGGGG TGCTGGGGTG AGGACACAAG GCTTCCCTGA GCCCACACAA 
AGCCAGTCAT GGCAAAGAGG TCAGCCTCGG CCTGGCCTTC CAGGGCCCTC AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_016938329.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016793818.1 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X9, mRNA.

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CloneID	OPc77422
Clone ID Related Accession (Same CDS sequence)	XM_016938329.1
Accession Version	XM_016938329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGACGTCTC AGGAAACTGG 
GGGCAACCTG GGGGGAGCCC CGTGGGCTGT GGGAACCCCT GCCCCCGGCT TGGAAGCTGC 
CCACGGTGGC CTGGTTGCAG CCTGGCAGAT GAGGGGTACA TCACTACTCA GAATAAACGG 
CCCCCTCTGC ACCCACCTGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016793818.1
CDS	146..4249
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLHHLPFQGEPQSLATLYQLVSQACESAIQTHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRDVSGNWGQPGGSPVGCGNPCPRLGSCPRWPGCSLADEGYITTQNKRPPLHPPV

Target ORF information:

RefSeq Version	XM_016938329.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016938329.1

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGACGTCTC AGGAAACTGG 
GGGCAACCTG GGGGGAGCCC CGTGGGCTGT GGGAACCCCT GCCCCCGGCT TGGAAGCTGC 
CCACGGTGGC CTGGTTGCAG CCTGGCAGAT GAGGGGTACA TCACTACTCA GAATAAACGG 
CCCCCTCTGC ACCCACCTGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_016938330.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016793819.1 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OPc77423
Clone ID Related Accession (Same CDS sequence)	XM_016938330.1
Accession Version	XM_016938330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTC ACGTGTCAAA GTTCGACTCC TTCCACAAGA 
ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT GAGCCCCTGG 
GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT GGTGGCCGCC 
ACCTTGAGGG CAGAGACGTC TCAGGAAACT GGGGGCAACC TGGGGGGAGC CCCGTGGGCT 
GTGGGAACCC CTGCCCCCGG CTTGGAAGCT GCCCACGGTG GCCTGGTTGC AGCCTGGCAG 
ATGAGGGGTA CATCACTACT CAGAATAAAC GGCCCCCTCT GCACCCACCT GTCTAGACGA 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016793819.1
CDS	147..4172
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAETSQETGGNLGGAPWAVGTPAPGLEAAHGGLVAAWQMRGTSLLRINGPLCTHLSRRG

Target ORF information:

RefSeq Version	XM_016938330.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016938330.1

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTC ACGTGTCAAA GTTCGACTCC TTCCACAAGA 
ACACCAGGCC CCCCATGCCC ATCTTGGAGC AGAAGCTGGA GCCGGGCACT GAGCCCCTGG 
GGTCCCCTGG CACCCGCAGC AAGGCCCTGG TTCCTGGAGA ATGGGGGCGT GGTGGCCGCC 
ACCTTGAGGG CAGAGACGTC TCAGGAAACT GGGGGCAACC TGGGGGGAGC CCCGTGGGCT 
GTGGGAACCC CTGCCCCCGG CTTGGAAGCT GCCCACGGTG GCCTGGTTGC AGCCTGGCAG 
ATGAGGGGTA CATCACTACT CAGAATAAAC GGCCCCCTCT GCACCCACCT GTCTAGACGA 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437624.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435899.2 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X12, mRNA.

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CloneID	OPc77424
Clone ID Related Accession (Same CDS sequence)	XM_009437624.2
Accession Version	XM_009437624.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437624.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGGGAGCC TGGAGCTGTT 
GGTCAGACGG GCAGCCCTGG GCAGCAGGGG GCTAGCACCC AGGGCCTCTG GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435899.2
CDS	145..4161
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLHHLPFQGEPQSLATLYQLVSQACESAIQTHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGEPGAVGQTGSPGQQGASTQGLWE

Target ORF information:

RefSeq Version	XM_009437624.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437624.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGCTG CACCACCTTC CCTTCCAGGG CGAGCCGCAG 
TCCCTTGCCA CCCTCTACCA GCTTGTGAGC CAGGCCTGTG AGTCTGCCAT TCAGACTCAC 
GTGTCAAAGT TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG 
AAGCTGGAGC CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT 
CCTGGAGAAT GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGGGAGCC TGGAGCTGTT 
GGTCAGACGG GCAGCCCTGG GCAGCAGGGG GCTAGCACCC AGGGCCTCTG GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437625.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435900.2 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X13, mRNA.

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CloneID	OPc77425
Clone ID Related Accession (Same CDS sequence)	XM_009437625.2
Accession Version	XM_009437625.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTGTGA GTCTGCCATT CAGACTCACG TGTCAAAGTT CGACTCCTTC 
CACAAGAACA CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG 
CCCCTGGGGT CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT 
GGCCGCCACC TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG 
CAGCAGGGGG CTAGCACCCA GGGCCTCTGG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435900.2
CDS	144..4139
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKGEPQSLATLYQLVSQACESAIQTHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGEPGAVGQTGSPGQQGASTQGLWE

Target ORF information:

RefSeq Version	XM_009437625.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437625.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTGTGA GTCTGCCATT CAGACTCACG TGTCAAAGTT CGACTCCTTC 
CACAAGAACA CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG 
CCCCTGGGGT CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT 
GGCCGCCACC TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGACGGG CAGCCCTGGG 
CAGCAGGGGG CTAGCACCCA GGGCCTCTGG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437626.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435901.2 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OPc77426
Clone ID Related Accession (Same CDS sequence)	XM_009437626.2
Accession Version	XM_009437626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTCTCA CGTGTCAAAG TTCGACTCCT TCCACAAGAA CACCAGGCCC 
CCCATGCCCA TCTTGGAGCA GAAGCTGGAG CCGGGCACTG AGCCCCTGGG GTCCCCTGGC 
ACCCGCAGCA AGGCCCTGGT TCCTGGAGAA TGGGGGCGTG GTGGCCGCCA CCTTGAGGGC 
AGAGGGGAGC CTGGAGCTGT TGGTCAGACG GGCAGCCCTG GGCAGCAGGG GGCTAGCACC 
CAGGGCCTCT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435901.2
CDS	145..4122
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYNVTILAYYRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGHVLHYWLTYAPASGLGPEKSVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKGEPQSLATLYQLVSQASHVSKFDSFHKNTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGRGEPGAVGQTGSPGQQGASTQGLWE

Target ORF information:

RefSeq Version	XM_009437626.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437626.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG 
TACAACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC 
CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG 
ACCCCCGACA GCCTGCAGGT CAGCTGGACA CCCCCGCTTG GCCACGTGCT CCATTACTGG 
CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCTGAGAAAT CCGTCTCTGT GCCAGCACCC 
AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGCTCTCA 
GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA TGGGCCAGAC AGCAGCCTGC 
CCAGCCCTCC GCCCCGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG 
GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG 
ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCCTC 
CCAGCCCCCC TGCCTCCAGA GCACACCATC GTCTTCCTTC TGCGCCTACT TCCCGAGACA 
CCCCGCGAGG CCTTCGCGCT GTGGCAGATG ACGGCCGAGG ACTTCCAGCC CCTCCTTGGG 
GTTCTGCTGG ATGCCGGGAG GAAGTCCCTG ACCTACTTCC ACCGCGACCC CAGGGCTGCC 
TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC 
AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG 
GCTGAGCGGC CCCTTGGGGA GATGAGCAGC CCACCCGCTG CCGGCTTCGT CACGCTGGGG 
AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG TGTTTCAGCT CCAGATGCTG 
CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG 
AGGGATGGAG AGACCTGCCC GGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT 
GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG 
CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT 
CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGCG CAGTCCAGGG GCCTGTGGGT 
CCACCAGGGG TCAAAGGAGA GAAAGGAGAC CATGGGCTCC CAGGCTTGCA GGGCCACCCC 
GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG 
GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG 
GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA 
GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG 
CTTGTGAGCC AGGCCTCTCA CGTGTCAAAG TTCGACTCCT TCCACAAGAA CACCAGGCCC 
CCCATGCCCA TCTTGGAGCA GAAGCTGGAG CCGGGCACTG AGCCCCTGGG GTCCCCTGGC 
ACCCGCAGCA AGGCCCTGGT TCCTGGAGAA TGGGGGCGTG GTGGCCGCCA CCTTGAGGGC 
AGAGGGGAGC CTGGAGCTGT TGGTCAGACG GGCAGCCCTG GGCAGCAGGG GGCTAGCACC 
CAGGGCCTCT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_009437623.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009435898.1 Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X10, mRNA.

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CloneID	OPc47818
Clone ID Related Accession (Same CDS sequence)	XM_009437623.2
Accession Version	XM_009437623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-1(XX) chain isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006487.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009437623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
GTCTCTGTGC CAGCACCCAG GAGCCACGTG ACACTGCCCG ACCTGCAGGC AGCCACGAAG 
TACAGGGTCC TGCTCTCAGC TATCTATGCA GCAGGCAGGA GTGAGGCTGT GTCTGCCATG 
GGCCAGACAG CAGCCTGCCC AGCCCTCCGC CCCGACGGCT CCCTCCCAGG GTTTGACCTG 
ATGGTGGCCT TCAGCCTGGT GGAAAAGGCT TATGCGTCCA TCCGGGGCGT GGCCATGGAG 
CCCTCTGCCT TCGGTGGGAC CCCGACCTTC ACGCTCTTCA AGGACGCCCA GCTGACAAGA 
CGGGTCAGTG ACGTCCTCCC AGCCCCCCTG CCTCCAGAGC ACACCATCGT CTTCCTTCTG 
CGCCTACTTC CCGAGACACC CCGCGAGGCC TTCGCGCTGT GGCAGATGAC GGCCGAGGAC 
TTCCAGCCCC TCCTTGGGGT TCTGCTGGAT GCCGGGAGGA AGTCCCTGAC CTACTTCCAC 
CGCGACCCCA GGGCTGCCTT GCAGGAGGCC ACCTTCGACC CGCAGGAAGT GAGGAAGATT 
TTCTTCGGGA GCTTCCACAA GGTGCACGTG GCTGTGGGCC GCTCCAAGGT CAGGCTCTAT 
GTGGACTGCC GGAAGGTGGC TGAGCGGCCC CTTGGGGAGA TGAGCAGCCC ACCCGCTGCC 
GGCTTCGTCA CGCTGGGGAG GCTGGCCAAG GCCAGGGGCC CCCGGAGCAG TTCGGCCGTG 
TTTCAGCTCC AGATGCTGCA GATCGTGTGC AGTGACACCT GGGCCGATGA GGACCGGTGC 
TGTGAGCTCC CTGCCTCGAG GGATGGAGAG ACCTGCCCGG CCTTCGTGTC TGCCTGTTCC 
TGTTCCTCAG AGACCCCTGG GCCCCCAGGA CCTCAAGGAC CCCCAGGCCT CCCTGGGAGG 
AATGGCACCC CAGGAGAGCA GGGCTTCCCA GGGCCCAGGG GAGAGCCCGG GCCACCCGGA 
CAGATGGGAC CAGAAGGTCC TGGAGGCCAG CAGGGCTCGC CGGGGACCCA GGGCCGCGCA 
GTCCAGGGGC CTGTGGGTCC ACCAGGGGTC AAAGGAGAGA AAGGAGACCA TGGGCTCCCA 
GGCTTGCAGG GCCACCCCGG CCACCAGGGC ATCCCCGGGA GAGTTGGCCT CCAGGGACCA 
AAGGGAATGA GAGGCCTGGA GGGAACTGCT GGCCTGCCTG GACCCCCTGG CCCCAGGGGG 
TTCCAGGGCA TGGCAGGGGC CAGGGGCACT AGTGGAGAGC GAGGACCTCC AGGGACCGTG 
GGGCCCACAG GACTGCCAGG GCCCAAAGGG GAACGAGGAG AGAAGCTCAC GTGTCAAAGT 
TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG AAGCTGGAGC 
CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT CCTGGAGAAT 
GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGGGAGCC TGGAGCTGTT GGTCAGACGG 
GCAGCCCTGG GCAGCAGGGG GCTAGCACCC AGGGCCTCTG GGAGTGACAG GACATTTTCT 
GCACTGCCCC GAGGAACGCT GAGCCTTCCT CCCTGGGTTT CTCTGGACAC CGAGAGCGAC 
CACATCCTGG AGAAGCCAGG AGAAAAGCTC AGGAAGAGCC TGCAGGTGGA AGGAGAGAGA 
AGCAGCGGCC TCGGCCAAGG CCCACCCCAT GCTCTTGGCT CTGTAGCATT TCCAAGTTCA 
GATAAACCCC TGAGTGCTCA CCCAGCCAAG CGAGGCGGGA GTCATTTCCT GGGAGAGTGT 
CCCCCACCCT GGCTCCGTAC ACCCCACCTT GAGGCCCTGC AGCTTCAGCC GCAGTGGGGA 
AGGGGAGGGG CTGGGTTGAT GGCGAATTGG GTTCAGGAGG GCAGCCGTGC CCTGTGGCCA 
TCTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009435898.1
CDS	145..4173
Translation	MSSGAPAHLGLCLWLWLGATLGREQVQASGLLRLSVLPEDRLQMKWRESEGSGLGYLVQVKPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSSLDRSSQRPLGSGAPEPTPSHMGSPDPEQASEPQVAFTPSQDPPTPGGSEWRETGPQFRCLPPVPADMVFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKEQVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPVAGLRPEAAKVVILVTDGKSQDDVHTAARVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGTLAGLLSRLICQRLQGGSPRQGPAAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPREVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTPRTIHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLRGPEGSEARGIHARTPALAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSGQTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSVTELPGDAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEVSVPAPRSHVTLPDLQAATKYRVLLSAIYAAGRSEAVSAMGQTAACPALRPDGSLPGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVLPAPLPPEHTIVFLLRLLPETPREAFALWQMTAEDFQPLLGVLLDAGRKSLTYFHRDPRAALQEATFDPQEVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMSSPPAAGFVTLGRLAKARGPRSSSAVFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPPGLPGRNGTPGEQGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRAVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLEGTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKLTCQSSTPSTRTPGPPCPSWSRSWSRALSPWGPLAPAARPWFLENGGVVAATLRAEGSLELLVRRAALGSRGLAPRASGSDRTFSALPRGTLSLPPWVSLDTESDHILEKPGEKLRKSLQVEGERSSGLGQGPPHALGSVAFPSSDKPLSAHPAKRGGSHFLGECPPPWLRTPHLEALQLQPQWGRGGAGLMANWVQEGSRALWPSQ

Target ORF information:

RefSeq Version	XM_009437623.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type XX alpha 1 (COL20A1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009437623.2

ORF Insert Sequence:

ATGAGCTCCG GAGCCCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
CTGGGAAGAG AGCAAGTTCA AGCAAGTGGC CTCCTGAGGC TGTCTGTGCT GCCTGAGGAC 
CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG 
AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC 
ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC 
TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC 
CTGGACAGAA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC 
ATGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG 
GATCCGCCCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG 
CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGTAT TGGCCACAGT 
CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGCGTCATCG CACCCTTTGA AATCGGGCCG 
GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG 
AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG 
GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGTG 
GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG 
GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG 
GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC 
ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTTGGCACAC TGGCTGGCCT GCTCAGCCGT 
CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA 
GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC 
ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA 
GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GGCCCGCCGC CTCCACGGAG 
CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC 
GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG 
CTGACCCTGG CCGCAGTGAC GCCCAGAACC ATCCACCTCA CCTGGCAGCC CTCGGCCGGG 
GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAGGA GGAGGAGCGA 
GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT 
GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCATGCC 
AGGACCCCCG CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG 
GCACGAGTGT TCTGGGAGGG TGCCCCAAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTC 
TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG 
CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG 
GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG 
CTGTCCGTGA CGGAGCTGCC AGGCGACGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG 
TCTGGCGTGC TTGTCTACCA GATCACATGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG 
GTCTCTGTGC CAGCACCCAG GAGCCACGTG ACACTGCCCG ACCTGCAGGC AGCCACGAAG 
TACAGGGTCC TGCTCTCAGC TATCTATGCA GCAGGCAGGA GTGAGGCTGT GTCTGCCATG 
GGCCAGACAG CAGCCTGCCC AGCCCTCCGC CCCGACGGCT CCCTCCCAGG GTTTGACCTG 
ATGGTGGCCT TCAGCCTGGT GGAAAAGGCT TATGCGTCCA TCCGGGGCGT GGCCATGGAG 
CCCTCTGCCT TCGGTGGGAC CCCGACCTTC ACGCTCTTCA AGGACGCCCA GCTGACAAGA 
CGGGTCAGTG ACGTCCTCCC AGCCCCCCTG CCTCCAGAGC ACACCATCGT CTTCCTTCTG 
CGCCTACTTC CCGAGACACC CCGCGAGGCC TTCGCGCTGT GGCAGATGAC GGCCGAGGAC 
TTCCAGCCCC TCCTTGGGGT TCTGCTGGAT GCCGGGAGGA AGTCCCTGAC CTACTTCCAC 
CGCGACCCCA GGGCTGCCTT GCAGGAGGCC ACCTTCGACC CGCAGGAAGT GAGGAAGATT 
TTCTTCGGGA GCTTCCACAA GGTGCACGTG GCTGTGGGCC GCTCCAAGGT CAGGCTCTAT 
GTGGACTGCC GGAAGGTGGC TGAGCGGCCC CTTGGGGAGA TGAGCAGCCC ACCCGCTGCC 
GGCTTCGTCA CGCTGGGGAG GCTGGCCAAG GCCAGGGGCC CCCGGAGCAG TTCGGCCGTG 
TTTCAGCTCC AGATGCTGCA GATCGTGTGC AGTGACACCT GGGCCGATGA GGACCGGTGC 
TGTGAGCTCC CTGCCTCGAG GGATGGAGAG ACCTGCCCGG CCTTCGTGTC TGCCTGTTCC 
TGTTCCTCAG AGACCCCTGG GCCCCCAGGA CCTCAAGGAC CCCCAGGCCT CCCTGGGAGG 
AATGGCACCC CAGGAGAGCA GGGCTTCCCA GGGCCCAGGG GAGAGCCCGG GCCACCCGGA 
CAGATGGGAC CAGAAGGTCC TGGAGGCCAG CAGGGCTCGC CGGGGACCCA GGGCCGCGCA 
GTCCAGGGGC CTGTGGGTCC ACCAGGGGTC AAAGGAGAGA AAGGAGACCA TGGGCTCCCA 
GGCTTGCAGG GCCACCCCGG CCACCAGGGC ATCCCCGGGA GAGTTGGCCT CCAGGGACCA 
AAGGGAATGA GAGGCCTGGA GGGAACTGCT GGCCTGCCTG GACCCCCTGG CCCCAGGGGG 
TTCCAGGGCA TGGCAGGGGC CAGGGGCACT AGTGGAGAGC GAGGACCTCC AGGGACCGTG 
GGGCCCACAG GACTGCCAGG GCCCAAAGGG GAACGAGGAG AGAAGCTCAC GTGTCAAAGT 
TCGACTCCTT CCACAAGAAC ACCAGGCCCC CCATGCCCAT CTTGGAGCAG AAGCTGGAGC 
CGGGCACTGA GCCCCTGGGG TCCCCTGGCA CCCGCAGCAA GGCCCTGGTT CCTGGAGAAT 
GGGGGCGTGG TGGCCGCCAC CTTGAGGGCA GAGGGGAGCC TGGAGCTGTT GGTCAGACGG 
GCAGCCCTGG GCAGCAGGGG GCTAGCACCC AGGGCCTCTG GGAGTGACAG GACATTTTCT 
GCACTGCCCC GAGGAACGCT GAGCCTTCCT CCCTGGGTTT CTCTGGACAC CGAGAGCGAC 
CACATCCTGG AGAAGCCAGG AGAAAAGCTC AGGAAGAGCC TGCAGGTGGA AGGAGAGAGA 
AGCAGCGGCC TCGGCCAAGG CCCACCCCAT GCTCTTGGCT CTGTAGCATT TCCAAGTTCA 
GATAAACCCC TGAGTGCTCA CCCAGCCAAG CGAGGCGGGA GTCATTTCCT GGGAGAGTGT 
CCCCCACCCT GGCTCCGTAC ACCCCACCTT GAGGCCCTGC AGCTTCAGCC GCAGTGGGGA 
AGGGGAGGGG CTGGGTTGAT GGCGAATTGG GTTCAGGAGG GCAGCCGTGC CCTGTGGCCA 
TCTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL20A1
Entrez Gene ID	739138
Full Name	collagen type XX alpha 1 chain
General protein information	Preferred Names collagen type XX alpha 1 chain Names collagen alpha-1(XX) chain collagen, type XX, alpha 1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Rattus norvegicus (Norway rat)	Col20a1	XP_006235874.1
Homo sapiens (human)	COL20A1	NP_065933.2
Pan troglodytes (chimpanzee)	COL20A1	XP_001148451.1
Bos taurus (cattle)	COL20A1	XP_002692297.2
Mus musculus (house mouse)	Col20a1	NP_082794.1
Gallus gallus (chicken)	COL20A1	NP_001004392.1
Canis lupus familiaris (dog)	COL20A1	XP_543096.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL20A1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

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